AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038278

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038278 MtrunA17_Chr7g0244741 89.333 75 8 0 4 78 44 118 1.02e-36 121
MsaG038278 MtrunA17_Chr7g0244681 70.513 78 21 1 1 78 41 116 5.06e-35 116
MsaG038278 MtrunA17_Chr7g0244821 74.667 75 16 2 4 78 44 115 1.41e-33 113
MsaG038278 MtrunA17_Chr1g0165761 79.032 62 12 1 17 78 56 116 2.44e-30 105
MsaG038278 MtrunA17_Chr7g0244721 72.581 62 17 0 17 78 56 117 4.01e-29 102
MsaG038278 MtrunA17_Chr7g0244791 60.274 73 27 1 6 78 51 121 1.94e-24 90.1
MsaG038278 MtrunA17_Chr1g0165751 54.667 75 33 1 22 96 61 134 5.25e-21 81.6
MsaG038278 MtrunA17_Chr7g0244811 53.731 67 31 0 11 77 51 117 7.45e-21 80.9
MsaG038278 MtrunA17_Chr7g0244761 59.677 62 25 0 17 78 80 141 8.83e-21 81.3
MsaG038278 MtrunA17_Chr7g0244691 50.685 73 34 1 6 78 46 116 1.64e-20 80.1
MsaG038278 MtrunA17_Chr7g0244831 47.945 73 36 1 7 79 47 117 6.08e-20 78.6
MsaG038278 MtrunA17_Chr7g0244841 48.571 70 35 1 9 78 47 115 1.63e-18 74.7
MsaG038278 MtrunA17_Chr7g0244751 50.746 67 33 0 13 79 51 117 3.97e-18 73.9
MsaG038278 MtrunA17_Chr7g0244671 43.421 76 43 0 13 88 52 127 9.54e-17 71.2
MsaG038278 MtrunA17_Chr4g0012901 41.176 68 40 0 14 81 54 121 2.15e-14 64.3
MsaG038278 MtrunA17_Chr4g0012891 42.857 70 36 1 13 78 54 123 9.14e-14 62.8
MsaG038278 MtrunA17_Chr4g0013001 44.776 67 37 0 13 79 47 113 2.89e-13 61.2
MsaG038278 MtrunA17_Chr4g0012951 46.970 66 35 0 13 78 47 112 3.05e-13 61.2
MsaG038278 MtrunA17_Chr4g0012961 45.455 66 36 0 13 78 47 112 3.18e-13 61.2
MsaG038278 MtrunA17_Chr4g0012991 45.455 66 36 0 13 78 102 167 4.43e-13 62.0
MsaG038278 MtrunA17_Chr7g0244641 42.105 76 43 1 4 79 44 118 2.00e-11 56.6
MsaG038278 MtrunA17_Chr3g0099411 62.791 43 16 0 74 116 14 56 5.25e-11 57.4
MsaG038278 MtrunA17_Chr1g0206721 62.791 43 16 0 74 116 14 56 8.00e-11 57.0
MsaG038278 MtrunA17_Chr7g0244731 52.830 53 23 1 7 59 76 126 8.80e-11 55.8
MsaG038278 MtrunA17_Chr1g0149391 62.791 43 16 0 74 116 14 56 9.80e-11 56.6
MsaG038278 MtrunA17_Chr8g0355091 62.791 43 16 0 74 116 14 56 9.80e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038278 AT5G59320.1 45.455 66 36 0 14 79 48 113 5.30e-12 58.5
MsaG038278 AT3G08770.2 40.260 77 42 2 8 82 40 114 1.84e-11 57.0
MsaG038278 AT3G51590.1 45.205 73 37 1 5 77 43 112 3.68e-11 56.2
MsaG038278 AT3G08770.1 41.667 72 38 2 8 77 40 109 9.15e-11 55.1

Find 32 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGACGATCTTGGGGAGTTT+TGG 0.263906 7:-74512427 None:intergenic
ATGTCAACACCACAGTCTTT+AGG 0.283593 7:-74512522 None:intergenic
ACCGCATTGCAACATGGTTC+AGG 0.289771 7:-74512384 None:intergenic
GCAAACATCTCGACGATCTT+GGG 0.310329 7:-74512436 None:intergenic
TGTCAACACCACAGTCTTTA+GGG 0.317231 7:-74512521 None:intergenic
CGCAAACATCTCGACGATCT+TGG 0.319102 7:-74512437 None:intergenic
TACTTGCTGCAGTCCATGTC+AGG 0.352769 7:-74512561 None:intergenic
TCGATGATGGGCGGCATACC+TGG 0.388848 7:+74512471 MsaT038278.1:CDS
CGGTTGCCTCAAATCGATGA+TGG 0.452362 7:+74512458 MsaT038278.1:CDS
CCGCATTGCAACATGGTTCA+GGG 0.458475 7:-74512383 None:intergenic
CAAGATCGTCGAGATGTTTG+CGG 0.467447 7:+74512438 MsaT038278.1:CDS
GGGGAGAGATGGGTGGACCT+GGG 0.494021 7:-74512362 None:intergenic
TTGCTCTTGTTATTCTCACT+TGG 0.500307 7:+74514135 MsaT038278.1:CDS
TTAGGGAGGGTAGCAACAGC+AGG 0.512840 7:-74512504 None:intergenic
AAAGACTGTGGTGTTGACAT+AGG 0.556773 7:+74512525 MsaT038278.1:CDS
TGGGTGTGTTGCGTCACGCA+TGG 0.562334 7:-74512343 None:intergenic
CTATATTATTAGCCCTGACA+TGG 0.578198 7:+74512548 MsaT038278.1:CDS
ACAGCAGGAAGATTGAGTCC+AGG 0.592974 7:-74512489 None:intergenic
GGTTGCCTCAAATCGATGAT+GGG 0.597219 7:+74512459 MsaT038278.1:CDS
TGCCGCCCATCATCGATTTG+AGG 0.604592 7:-74512464 None:intergenic
CGCATTGCAACATGGTTCAG+GGG 0.606881 7:-74512382 None:intergenic
AGCAACGTCTACACCACGAA+AGG 0.631108 7:-74514117 None:intergenic
GTCTTAACCGCATTGCAACA+TGG 0.632276 7:-74512390 None:intergenic
AACACCACAGTCTTTAGGGA+GGG 0.649527 7:-74512517 None:intergenic
ATGCGTGACGCAACACACCC+AGG 0.651816 7:+74512345 MsaT038278.1:CDS
ACTTGCTGCAGTCCATGTCA+GGG 0.654891 7:-74512560 None:intergenic
TGCCTCAAATCGATGATGGG+CGG 0.660181 7:+74512462 MsaT038278.1:CDS
CAAACATCTCGACGATCTTG+GGG 0.662281 7:-74512435 None:intergenic
GCTACCCTCCCTAAAGACTG+TGG 0.674647 7:+74512513 MsaT038278.1:CDS
CCCTGAACCATGTTGCAATG+CGG 0.675174 7:+74512383 MsaT038278.1:CDS
GCATTGCAACATGGTTCAGG+GGG 0.680076 7:-74512381 None:intergenic
CAACACCACAGTCTTTAGGG+AGG 0.690471 7:-74512518 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 74512345 74514160 74512345 ID=MsaG038278
Chr7 mRNA 74512345 74514160 74512345 ID=MsaT038278.1;Parent=MsaG038278
Chr7 exon 74512345 74512580 74512345 ID=MsaT038278.1.exon1;Parent=MsaT038278.1
Chr7 CDS 74512345 74512580 74512345 ID=cds.MsaT038278.1;Parent=MsaT038278.1
Chr7 exon 74514043 74514160 74514043 ID=MsaT038278.1.exon2;Parent=MsaT038278.1
Chr7 CDS 74514043 74514160 74514043 ID=cds.MsaT038278.1;Parent=MsaT038278.1
Gene Sequence

>MsaT038278.1

ATGCGTGACGCAACACACCCAGGTCCACCCATCTCTCCCCCTGAACCATGTTGCAATGCGGTTAAGACTTTAAATGAGCAATCCAAAACTCCCCAAGATCGTCGAGATGTTTGCGGTTGCCTCAAATCGATGATGGGCGGCATACCTGGACTCAATCTTCCTGCTGTTGCTACCCTCCCTAAAGACTGTGGTGTTGACATAGGCTATATTATTAGCCCTGACATGGACTGCAGCAACAAGGAGAGTGATCTGCAAATGACATTCTCAAAGCGTCGTAGTAGTCTCTTCAAGAAAGCTAGTGAGTTTTGCACCTTTCGTGGTGTAGACGTTGCTCTTGTTATTCTCACTTGGTGA
Protein sequence

>MsaT038278.1

MRDATHPGPPISPPEPCCNAVKTLNEQSKTPQDRRDVCGCLKSMMGGIPGLNLPAVATLPKDCGVDIGYIISPDMDCSNKESDLQMTFSKRRSSLFKKASEFCTFRGVDVALVILTW*