Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG038301 | XP_039682845.1 | 94.697 | 132 | 7 | 0 | 26 | 157 | 1 | 132 | 2.97e-83 | 258 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG038301 | tr|G7I4G2|G7I4G2_MEDTR | 91.045 | 134 | 12 | 0 | 24 | 157 | 26 | 159 | 5.98e-82 | 251 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG038301 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG038301 | MtrunA17_Chr2g0289081 | 76.471 | 102 | 23 | 1 | 24 | 124 | 19 | 120 | 5.80e-49 | 154 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTCCAAAACCTTCTTTCT+TGG | 0.206214 | 7:-74718341 | None:intergenic |
| TATTTGTCCTATTGTTGATT+TGG | 0.256460 | 7:+74718273 | MsaT038301.1:CDS |
| AAGCAGGGAAGAGAAGTAAA+TGG | 0.304863 | 7:+74718506 | MsaT038301.1:CDS |
| CCTAACCTAATTGCGGCATT+TGG | 0.319650 | 7:-74716913 | None:intergenic |
| ACAATCCAAATGCCGCAATT+AGG | 0.351873 | 7:+74716908 | MsaT038301.1:CDS |
| TTTGCCAAGAAAGAAGGTTT+TGG | 0.354079 | 7:+74718337 | MsaT038301.1:CDS |
| TCAAATTCCAAATCAACAAT+AGG | 0.420244 | 7:-74718280 | None:intergenic |
| GAAGGTACACATATAGTAAA+GGG | 0.439216 | 7:+74718412 | MsaT038301.1:CDS |
| TCACTTACTGTTTCAAAAGC+AGG | 0.452498 | 7:+74718490 | MsaT038301.1:CDS |
| ACATCGTTTGCCAAGAAAGA+AGG | 0.462978 | 7:+74718331 | MsaT038301.1:CDS |
| ATAGGACAAATATTAGCTTC+AGG | 0.470032 | 7:-74718262 | None:intergenic |
| AGCAGGGAAGAGAAGTAAAT+GGG | 0.489503 | 7:+74718507 | MsaT038301.1:CDS |
| TGAAGGTACACATATAGTAA+AGG | 0.494494 | 7:+74718411 | MsaT038301.1:CDS |
| AATATTAGCTTCAGGCGACT+TGG | 0.496239 | 7:-74718254 | None:intergenic |
| GTAAAGGGTGAAAATGAAGA+AGG | 0.507431 | 7:+74718427 | MsaT038301.1:CDS |
| TGCTTTACAATCAGTTCTTG+AGG | 0.537334 | 7:-74718467 | None:intergenic |
| ATGCCGCAATTAGGTTAGGC+AGG | 0.539897 | 7:+74716917 | MsaT038301.1:CDS |
| CACTTACTGTTTCAAAAGCA+GGG | 0.542698 | 7:+74718491 | MsaT038301.1:CDS |
| TAAAGGGTGAAAATGAAGAA+GGG | 0.554329 | 7:+74718428 | MsaT038301.1:CDS |
| ATGTTTGTACGCGCTAATGA+AGG | 0.560428 | 7:+74718394 | MsaT038301.1:CDS |
| CCAAATGCCGCAATTAGGTT+AGG | 0.568264 | 7:+74716913 | MsaT038301.1:CDS |
| ACACATCATCAACTATCTCA+TGG | 0.582009 | 7:-74718201 | None:intergenic |
| TTACCTGCCTAACCTAATTG+CGG | 0.648113 | 7:-74716920 | None:intergenic |
| AAGATGAGTCAAAGAATGGG+AGG | 0.654186 | 7:+74716180 | None:intergenic |
| GTACATGCATAATGAGTGTG+TGG | 0.676324 | 7:+74718150 | MsaT038301.1:CDS |
| GTCTTCTAAACACTCAACAG+TGG | 0.678815 | 7:-74718179 | None:intergenic |
| TTTGGAATTTGACTCCATTG+CGG | 0.690541 | 7:+74718291 | MsaT038301.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 74716195 | 74718573 | 74716195 | ID=MsaG038301 |
| Chr7 | mRNA | 74716195 | 74718573 | 74716195 | ID=MsaT038301.1;Parent=MsaG038301 |
| Chr7 | exon | 74716195 | 74716201 | 74716195 | ID=MsaT038301.1.exon1;Parent=MsaT038301.1 |
| Chr7 | CDS | 74716195 | 74716201 | 74716195 | ID=cds.MsaT038301.1;Parent=MsaT038301.1 |
| Chr7 | exon | 74716875 | 74716938 | 74716875 | ID=MsaT038301.1.exon2;Parent=MsaT038301.1 |
| Chr7 | CDS | 74716875 | 74716938 | 74716875 | ID=cds.MsaT038301.1;Parent=MsaT038301.1 |
| Chr7 | exon | 74718150 | 74718573 | 74718150 | ID=MsaT038301.1.exon3;Parent=MsaT038301.1 |
| Chr7 | CDS | 74718150 | 74718573 | 74718150 | ID=cds.MsaT038301.1;Parent=MsaT038301.1 |
| Gene Sequence |
| Protein sequence |
