AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038429

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038429 MtrunA17_Chr7g0246631 96.915 778 24 0 1 778 1 778 0.0 1568
MsaG038429 MtrunA17_Chr8g0350321 79.823 793 133 11 1 778 30 810 0.0 1268
MsaG038429 MtrunA17_Chr1g0203671 64.075 746 229 9 56 775 58 790 0.0 951
MsaG038429 MtrunA17_Chr2g0322731 54.787 752 305 14 37 777 41 768 0.0 776
MsaG038429 MtrunA17_Chr4g0024351 54.058 764 304 15 30 778 2 733 0.0 773
MsaG038429 MtrunA17_Chr6g0472471 51.733 779 326 17 27 774 6 765 0.0 767
MsaG038429 MtrunA17_Chr6g0481041 49.086 711 327 11 91 778 29 727 0.0 660
MsaG038429 MtrunA17_Chr5g0410711 44.177 747 370 19 55 774 52 778 0.0 602
MsaG038429 MtrunA17_Chr2g0331581 42.961 689 348 9 95 777 143 792 0.0 596
MsaG038429 MtrunA17_Chr5g0394111 40.423 710 334 14 74 774 17 646 1.00e-167 499
MsaG038429 MtrunA17_Chr7g0224451 42.339 496 214 10 85 572 10 441 2.99e-122 374
MsaG038429 MtrunA17_Chr2g0292731 37.037 486 293 8 295 774 70 548 1.92e-97 313
MsaG038429 MtrunA17_Chr2g0292741 36.864 491 282 12 295 774 62 535 3.68e-95 306
MsaG038429 MtrunA17_Chr7g0224481 41.344 387 164 7 85 465 10 339 2.98e-86 276
MsaG038429 MtrunA17_Chr7g0224491 45.171 321 138 10 468 776 1 295 1.64e-76 249
MsaG038429 MtrunA17_Chr2g0292761 31.331 616 336 23 170 774 1 540 7.76e-73 247
MsaG038429 MtrunA17_Chr5g0419821 54.098 122 55 1 91 211 33 154 6.75e-39 142
MsaG038429 MtrunA17_Chr2g0319671 30.961 281 147 5 290 538 19 284 1.10e-36 140
MsaG038429 MtrunA17_Chr2g0319701 36.752 234 137 6 549 778 1 227 1.19e-34 132
MsaG038429 MtrunA17_Chr2g0319681 34.335 233 146 4 549 778 1 229 3.47e-32 125
MsaG038429 MtrunA17_Chr2g0319691 40.945 127 68 2 403 528 1 121 1.88e-26 105
MsaG038429 MtrunA17_Chr1g0205981 34.259 216 88 8 146 339 1 184 6.97e-24 100
MsaG038429 MtrunA17_Chr7g0224461 47.967 123 47 4 664 776 1 116 1.52e-23 96.3
MsaG038429 MtrunA17_Chr2g0304191 36.957 138 73 4 297 427 1 131 6.14e-23 95.5
MsaG038429 MtrunA17_Chr7g0224431 35.220 159 59 2 317 475 6 120 9.29e-19 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038429 AT4G00730.1 69.845 776 203 12 11 778 50 802 0.0 1055
MsaG038429 AT3G61150.1 64.480 808 223 16 1 778 34 807 0.0 1008
MsaG038429 AT4G04890.2 54.886 747 279 13 55 777 26 738 0.0 793
MsaG038429 AT4G04890.1 54.886 747 279 13 55 777 26 738 0.0 793
MsaG038429 AT4G21750.2 55.556 729 266 11 84 777 55 760 0.0 776
MsaG038429 AT4G21750.1 55.556 729 266 11 84 777 55 760 0.0 776
MsaG038429 AT4G21750.3 55.556 729 266 11 84 777 55 760 0.0 776
MsaG038429 AT4G21750.4 55.556 729 266 11 84 777 55 760 0.0 776
MsaG038429 AT1G05230.8 53.215 731 307 11 50 778 23 720 0.0 762
MsaG038429 AT1G05230.4 53.215 731 307 11 50 778 23 720 0.0 762
MsaG038429 AT1G05230.9 53.215 731 307 11 50 778 23 720 0.0 762
MsaG038429 AT1G05230.10 53.215 731 307 11 50 778 23 720 0.0 762
MsaG038429 AT1G05230.7 53.215 731 307 11 50 778 23 720 0.0 762
MsaG038429 AT1G05230.11 53.215 731 307 11 50 778 23 720 0.0 762
MsaG038429 AT1G05230.2 53.215 731 307 11 50 778 23 720 0.0 762
MsaG038429 AT1G05230.6 53.215 731 307 11 50 778 23 720 0.0 762
MsaG038429 AT1G05230.5 53.215 731 307 11 50 778 23 720 0.0 762
MsaG038429 AT1G05230.1 53.215 731 307 11 50 778 23 720 0.0 762
MsaG038429 AT1G05230.3 53.215 731 305 12 50 778 23 718 0.0 758
MsaG038429 AT5G52170.3 53.398 721 265 13 61 775 24 679 0.0 745
MsaG038429 AT5G52170.1 53.398 721 265 13 61 775 24 679 0.0 745
MsaG038429 AT5G52170.2 53.030 726 265 14 61 775 24 684 0.0 739
MsaG038429 AT4G00730.2 66.226 530 150 11 11 532 50 558 0.0 677
MsaG038429 AT1G73360.1 48.429 700 327 12 91 774 32 713 0.0 625
MsaG038429 AT1G17920.2 46.286 700 323 14 94 778 24 685 0.0 612
MsaG038429 AT1G17920.1 46.286 700 323 14 94 778 24 685 0.0 612
MsaG038429 AT1G05230.12 51.480 608 266 10 50 655 23 603 0.0 608
MsaG038429 AT2G32370.1 43.966 696 343 11 91 778 68 724 0.0 577
MsaG038429 AT1G79840.1 41.917 699 346 12 88 777 98 745 0.0 557
MsaG038429 AT1G79840.3 41.917 699 346 12 88 777 40 687 0.0 556
MsaG038429 AT1G79840.6 41.917 699 346 12 88 777 40 687 0.0 556
MsaG038429 AT1G79840.2 41.917 699 346 12 88 777 127 774 0.0 555
MsaG038429 AT5G46880.3 41.922 718 375 19 84 774 104 806 2.10e-176 527
MsaG038429 AT5G46880.1 41.922 718 375 19 84 774 104 806 2.10e-176 527
MsaG038429 AT5G46880.2 41.922 718 375 19 84 774 104 806 2.10e-176 527
MsaG038429 AT4G25530.1 40.112 713 366 15 80 778 21 686 1.31e-164 492
MsaG038429 AT1G79840.4 41.549 568 283 9 88 651 98 620 3.83e-153 461
MsaG038429 AT4G17710.1 40.404 693 333 18 89 774 84 703 1.92e-149 454
MsaG038429 AT3G03260.1 36.746 713 383 18 92 776 24 696 6.70e-142 434
MsaG038429 AT5G17320.1 35.150 734 409 23 81 777 15 718 1.92e-125 392
MsaG038429 AT1G34650.1 34.318 711 411 21 98 777 23 708 1.49e-115 366
MsaG038429 AT1G79840.5 40.583 446 218 7 88 530 98 499 2.78e-107 338
MsaG038429 AT3G03260.2 35.714 546 306 13 92 623 24 538 3.41e-104 331
MsaG038429 AT5G07260.2 27.778 558 320 16 157 712 10 486 5.83e-54 196
MsaG038429 AT5G07260.1 27.679 560 323 16 157 712 10 491 5.87e-54 196
MsaG038429 AT1G30490.1 40.000 100 53 3 79 174 6 102 9.23e-13 72.4

Find 183 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTTGTGTTCTTCTATTT+TGG 0.092193 7:+76464935 None:intergenic
GGCTCTTGTTGAAACTTTAA+TGG 0.179447 7:-76464030 MsaT038429.1:CDS
TGGATTAGCAATGTTGAAAA+TGG 0.200597 7:-76464176 MsaT038429.1:CDS
CAGTTGTAGAGCACCATTTC+TGG 0.218972 7:+76463462 None:intergenic
ATGCCTTTAGGTCAAGATTT+TGG 0.233318 7:-76464362 MsaT038429.1:CDS
CTAAGCTTAGGCATGGGTTT+TGG 0.241414 7:-76461036 MsaT038429.1:CDS
TCAAACCATTATTTGTTTGA+TGG 0.255996 7:+76465300 None:intergenic
GATAACATGGATGTTATTTC+TGG 0.263046 7:-76465230 MsaT038429.1:CDS
GAGTTTGGTAACGACGTAAT+TGG 0.264874 7:+76464434 None:intergenic
GTGATATCCAGTGGAATAAA+TGG 0.276732 7:-76463487 MsaT038429.1:CDS
AATTTCCATCAAACAAATAA+TGG 0.293878 7:-76465305 MsaT038429.1:CDS
CACAAACTAATGAACCTCTT+TGG 0.303010 7:-76464196 MsaT038429.1:CDS
ACTTCAAGTTCTTTCTCCTT+TGG 0.311561 7:-76463280 MsaT038429.1:CDS
AAACAAGACAAGTCAAGTTT+TGG 0.322654 7:-76464960 MsaT038429.1:CDS
AGTTTCTCTGATGGTATCTA+TGG 0.338811 7:+76463182 None:intergenic
ACTTAGCAAAAGGCTTTGTT+TGG 0.341498 7:-76464983 MsaT038429.1:CDS
CCAGTTTCTAAACTCGAGTT+TGG 0.349356 7:+76464419 None:intergenic
TATGTTTCTTGAACTTGCTT+TGG 0.349553 7:-76464246 MsaT038429.1:CDS
GGAATGTGGGCAAGGACGTT+AGG 0.354651 7:-76460675 MsaT038429.1:CDS
AACTGTAGGAGGCTTCCTTC+TGG 0.355417 7:-76463135 MsaT038429.1:CDS
TTGCCTTCAACAATGCCTTT+AGG 0.357400 7:-76464374 MsaT038429.1:CDS
TTCATTACTACATGCATTGC+GGG 0.358765 7:+76459079 None:intergenic
GGTGCACAAATGGAACAAAT+TGG 0.362490 7:-76460704 MsaT038429.1:CDS
CACAAAAGTTGTTTGTCATT+CGG 0.369193 7:+76460746 None:intergenic
TGAACTTGCTTTGGCTGCAA+TGG 0.370269 7:-76464237 MsaT038429.1:CDS
AAATCCATGTTCAACTCATC+TGG 0.372663 7:-76465495 MsaT038429.1:CDS
TCGTTTATCAACTCCTACTT+TGG 0.381588 7:-76465520 MsaT038429.1:CDS
GAAATTTCACTAATAATTGC+TGG 0.383011 7:+76464548 None:intergenic
ATTGTTAGTACTCCTTGTAT+TGG 0.383234 7:-76464119 MsaT038429.1:CDS
GCCTCAGAAACAAAACCATT+TGG 0.385246 7:+76464089 None:intergenic
TGGTGATATGAAGAGATTAA+TGG 0.385985 7:-76465333 MsaT038429.1:CDS
GTCTATAGAGCTGGTGAACT+TGG 0.390142 7:+76461064 None:intergenic
GTGCATCGACGGTGCACAAA+TGG 0.392526 7:-76460714 MsaT038429.1:CDS
TTTGTTCCTCTTGTAATTGA+AGG 0.396389 7:+76464320 None:intergenic
TATGCCAAATGGTTACTCTA+AGG 0.401101 7:-76463097 MsaT038429.1:intron
AGCAGCATGCAGAAGGGGTA+TGG 0.402136 7:-76463221 MsaT038429.1:CDS
GAAGGCTATTCACAAGTATC+TGG 0.404248 7:+76458921 None:intergenic
TTGATTGTATCAGACTCAAT+TGG 0.408893 7:-76464678 MsaT038429.1:intron
GTTGCCATGGTCATGTCCTT+TGG 0.409163 7:+76460468 None:intergenic
AATTACAAGAGGAACAAATA+TGG 0.409950 7:-76464315 MsaT038429.1:CDS
GGAATAAATGGAACCAGAAA+TGG 0.415829 7:-76463475 MsaT038429.1:CDS
CCAAACTCGAGTTTAGAAAC+TGG 0.419938 7:-76464419 MsaT038429.1:CDS
ATTACCTTAGAGTAACCATT+TGG 0.422663 7:+76463093 None:intergenic
CTCCCGTCGCTATGTCCATC+TGG 0.423743 7:+76459004 None:intergenic
GAACATGAAAGCTGCAGATC+TGG 0.433822 7:-76465257 MsaT038429.1:CDS
TCTGGTTCCATTTATTCCAC+TGG 0.434725 7:+76463480 None:intergenic
TGTATTGGTTTGAAACCAAA+TGG 0.437986 7:-76464104 MsaT038429.1:CDS
AAGGAAGAGTGTGGATGATC+CGG 0.443006 7:-76460644 MsaT038429.1:CDS
CTCTTGCATTGGACCACCAT+TGG 0.446395 7:+76460504 None:intergenic
TGACCTAAAGGCATTGTTGA+AGG 0.455945 7:+76464371 None:intergenic
GGATGATCCGGGTGAGCCAC+CGG 0.456267 7:-76460632 MsaT038429.1:CDS
CAGAAATGGTGCTCTACAAC+TGG 0.456843 7:-76463461 MsaT038429.1:intron
GGTTGTGAGATTGCACTAGA+AGG 0.460615 7:+76465543 None:intergenic
GAAACAAGCACAGACGCATC+GGG 0.460821 7:-76459133 MsaT038429.1:CDS
GACATTCACATTGGCGTTGG+AGG 0.461547 7:+76460992 None:intergenic
AGAAAACTCACCTCACGAAC+AGG 0.469601 7:+76463255 None:intergenic
ACAATCCCCGGTGGCTCACC+CGG 0.478000 7:+76460625 None:intergenic
TTATGTTGCGCTTCTTCCGT+CGG 0.480355 7:-76459041 MsaT038429.1:CDS
AAAGACTTGAACTTAGCAAA+AGG 0.481146 7:-76464993 MsaT038429.1:CDS
TTAGTGCAGCAACCTCAGTT+TGG 0.483693 7:-76460600 MsaT038429.1:CDS
GAATGTGGGCAAGGACGTTA+GGG 0.488119 7:-76460674 MsaT038429.1:CDS
ACGAGAAGCTTAGAAGTGAA+TGG 0.489778 7:-76460537 MsaT038429.1:CDS
TATGTTGCGCTTCTTCCGTC+GGG 0.492269 7:-76459040 MsaT038429.1:CDS
GATGATCCGGGTGAGCCACC+GGG 0.500082 7:-76460631 MsaT038429.1:CDS
CAAATTGGATGCAGGGAATG+TGG 0.502022 7:-76460689 MsaT038429.1:CDS
AAATGGAACAAATTGGATGC+AGG 0.503062 7:-76460697 MsaT038429.1:CDS
ATACCTGAGTGTTCTCTAGC+GGG 0.506667 7:+76460943 None:intergenic
CTAGACATTCACATTGGCGT+TGG 0.506942 7:+76460989 None:intergenic
CACGGTGGAATCAGTGGAAA+CGG 0.508426 7:-76458885 MsaT038429.1:CDS
ATGCCAAAATCTTGACCTAA+AGG 0.510136 7:+76464359 None:intergenic
CCTCCTACAGTTAAGAAAAG+TGG 0.511269 7:+76463146 None:intergenic
TGCAGAAGGGGTATGGGCAG+TGG 0.512805 7:-76463214 MsaT038429.1:CDS
CGAGAAGCTTAGAAGTGAAT+GGG 0.514863 7:-76460536 MsaT038429.1:CDS
GGTGGAGCATGCAGAATATG+AGG 0.519622 7:-76461091 MsaT038429.1:CDS
TCCTCCACAATTTGAACACA+TGG 0.523795 7:+76464571 None:intergenic
TCCTTTGGTTCCTGTTCGTG+AGG 0.529142 7:-76463265 MsaT038429.1:CDS
GAGGCTTCCTTCTGGTTGTG+TGG 0.534040 7:-76463127 MsaT038429.1:CDS
TTCTAAGCTTCTCGTTGCGA+AGG 0.534552 7:+76460545 None:intergenic
ACCCTTGCTAAGCTTAGGCA+TGG 0.536023 7:-76461043 MsaT038429.1:CDS
CACCGTCGATGCACAAACTC+CGG 0.536497 7:+76460723 None:intergenic
CCGTGAGCTTTGCCGTCGGA+AGG 0.537799 7:+76458903 None:intergenic
CTCCTACAGTTAAGAAAAGT+GGG 0.539174 7:+76463147 None:intergenic
AGGAAGAGTGTGGATGATCC+GGG 0.542002 7:-76460643 MsaT038429.1:CDS
TCTGAGGCTTCTAGAGAAAG+TGG 0.543217 7:-76464074 MsaT038429.1:CDS
ACTGGATATCACTTCAGTTG+TGG 0.543692 7:+76463498 None:intergenic
TCAAGTTCTTGAATTTGTTG+AGG 0.544285 7:+76465149 None:intergenic
AACATGGATTTAACCAAAGT+AGG 0.544723 7:+76465507 None:intergenic
AAATAGAAGAACACAAATGA+AGG 0.546022 7:-76464932 MsaT038429.1:intron
TTTGGTTTCAAACCAATACA+AGG 0.548636 7:+76464107 None:intergenic
GCAGCATGCAGAAGGGGTAT+GGG 0.548640 7:-76463220 MsaT038429.1:CDS
CAGATCTGGTAGTGATAACA+TGG 0.549386 7:-76465243 MsaT038429.1:CDS
TGCCGGAGTTTGTGCATCGA+CGG 0.549484 7:-76460725 MsaT038429.1:CDS
CCCTTGCTAAGCTTAGGCAT+GGG 0.551648 7:-76461042 MsaT038429.1:CDS
GGTAATGACAAGGAAGAGTG+TGG 0.554273 7:-76460653 MsaT038429.1:CDS
ATGTGAGCCATCTCTTGCAT+TGG 0.555705 7:+76460493 None:intergenic
TCAACATTGCTAATCCAAAG+AGG 0.557396 7:+76464182 None:intergenic
TGCATACACTAGCTCGTAGG+AGG 0.558654 7:+76460437 None:intergenic
AGAAACAAGCACAGACGCAT+CGG 0.558699 7:-76459134 MsaT038429.1:CDS
ACATTCACATTGGCGTTGGA+GGG 0.559350 7:+76460993 None:intergenic
GGGTCGCAGCGGAGAGCGAG+TGG 0.562161 7:-76458965 MsaT038429.1:CDS
AACATATGATGCAGGTGACA+TGG 0.564953 7:-76461113 MsaT038429.1:intron
TTAGCAAGGGTCTATAGAGC+TGG 0.566123 7:+76461055 None:intergenic
TGTTGTTATCATAAACAGCT+TGG 0.566826 7:-76464051 MsaT038429.1:CDS
AAATTGGATGCAGGGAATGT+GGG 0.568174 7:-76460688 MsaT038429.1:CDS
ACCCATGCCTAAGCTTAGCA+AGG 0.568460 7:+76461041 None:intergenic
AATCCCATGTGTTCAAATTG+TGG 0.573172 7:-76464575 MsaT038429.1:CDS
CTGTTGTATAACAGAATCGA+TGG 0.574417 7:-76463555 MsaT038429.1:intron
GAGCTTCTTGCAATCACACA+AGG 0.574571 7:+76463520 None:intergenic
GTCGCTTGTTGTGTACGCGC+CGG 0.575675 7:-76459110 MsaT038429.1:CDS
GGTTTCGCCGTGTTACCAGA+TGG 0.577544 7:-76459019 MsaT038429.1:CDS
AATGGAACAAATTGGATGCA+GGG 0.583192 7:-76460696 MsaT038429.1:CDS
AATGGATGAGTTAGTGAAGA+TGG 0.583650 7:-76464219 MsaT038429.1:CDS
TGCATTGCGGGAATATCTAC+CGG 0.586205 7:+76459091 None:intergenic
GGAGTGGTAATCACGATGAC+GGG 0.588681 7:-76458985 MsaT038429.1:CDS
TAGCAATAAGTGCAGGAGGA+AGG 0.588813 7:-76460792 MsaT038429.1:CDS
GAGTGATGATGACATTAAGA+TGG 0.589154 7:+76460966 None:intergenic
CCTTCCGACGGCAAAGCTCA+CGG 0.589815 7:-76458903 MsaT038429.1:CDS
ACCATCAGAGAAACTTCAGC+TGG 0.590474 7:-76463174 MsaT038429.1:CDS
TACCAGATGGACATAGCGAC+GGG 0.591264 7:-76459006 MsaT038429.1:CDS
ACCAGCTGAAGTTTCTCTGA+TGG 0.591829 7:+76463173 None:intergenic
GCTGCACTAAGCACAATCCC+CGG 0.592750 7:+76460613 None:intergenic
AAACAAATAATGGTTTGAGA+AGG 0.592932 7:-76465295 MsaT038429.1:CDS
GTAACAAATAGTGATAGTAA+TGG 0.597005 7:-76464293 MsaT038429.1:CDS
CTATGTGTAGCAATAAGTGC+AGG 0.597052 7:-76460799 MsaT038429.1:intron
ACCTCACGAACAGGAACCAA+AGG 0.598440 7:+76463264 None:intergenic
TGGTGGTCCAATGCAAGAGA+TGG 0.598545 7:-76460500 MsaT038429.1:CDS
TTGCAAGCAGCATGCAGAAG+GGG 0.600217 7:-76463226 MsaT038429.1:CDS
GTGGTTCAAGATATGCCAAA+TGG 0.603358 7:-76463108 MsaT038429.1:CDS
GATGGACATAGCGACGGGAG+TGG 0.607388 7:-76459001 MsaT038429.1:CDS
GGATGCAGGGAATGTGGGCA+AGG 0.607975 7:-76460683 MsaT038429.1:CDS
GTTCCTCTTGTAATTGAAGG+TGG 0.608032 7:+76464323 None:intergenic
GACCGAGTTTGTACACTCGC+CGG 0.609622 7:-76464473 MsaT038429.1:CDS
AAAAGAAATTCTTAATCATG+AGG 0.610086 7:-76464153 MsaT038429.1:CDS
GGAGGGAGACACAGTTGCCA+TGG 0.612613 7:+76460455 None:intergenic
CCCATGCCTAAGCTTAGCAA+GGG 0.614032 7:+76461042 None:intergenic
GAATTTGTTGAGGAGTGTGT+CGG 0.614112 7:+76465159 None:intergenic
AAAGCTCACGGTGGAATCAG+TGG 0.620306 7:-76458891 MsaT038429.1:CDS
TCCACCGTGAGCTTTGCCGT+CGG 0.623528 7:+76458899 None:intergenic
GTCACGAGAATTCACTACTT+AGG 0.625972 7:-76464652 MsaT038429.1:CDS
TCACCACCTTCAATTACAAG+AGG 0.628235 7:-76464326 MsaT038429.1:CDS
GAATGGGACATACTCTCCAA+TGG 0.631124 7:-76460520 MsaT038429.1:CDS
GGGAGTGGTAATCACGATGA+CGG 0.631663 7:-76458986 MsaT038429.1:CDS
TTAATCATGAGGAATATACA+AGG 0.633009 7:-76464142 MsaT038429.1:CDS
TTTGCAAGCAGCATGCAGAA+GGG 0.633056 7:-76463227 MsaT038429.1:CDS
TAAACCAGATGAGTTGAACA+TGG 0.634149 7:+76465491 None:intergenic
TCTTGAACCACACAACCAGA+AGG 0.644378 7:+76463120 None:intergenic
CCCATGTGTTCAAATTGTGG+AGG 0.646740 7:-76464572 MsaT038429.1:CDS
TGCCGGCGAGTGTACAAACT+CGG 0.649600 7:+76464471 None:intergenic
GTTCATTACTACATGCATTG+CGG 0.652040 7:+76459078 None:intergenic
TTGGTAACGACGTAATTGGA+CGG 0.652910 7:+76464438 None:intergenic
ATATGCATACACTAGCTCGT+AGG 0.653193 7:+76460434 None:intergenic
TATAGACCCTTGCTAAGCTT+AGG 0.654709 7:-76461048 MsaT038429.1:CDS
ACATATGATGCAGGTGACAT+GGG 0.655326 7:-76461112 MsaT038429.1:intron
GCATACACTAGCTCGTAGGA+GGG 0.655354 7:+76460438 None:intergenic
GAGATGGCTCACATTGCCAA+AGG 0.656074 7:-76460484 MsaT038429.1:CDS
ATCACGATGACGGGTCGCAG+CGG 0.657275 7:-76458976 MsaT038429.1:CDS
GGTAACACGGCGAAACCCGA+CGG 0.657528 7:+76459025 None:intergenic
ACAACTGAAGTGATATCCAG+TGG 0.658205 7:-76463496 MsaT038429.1:CDS
AGATGGCTAGACATTCACAT+TGG 0.658957 7:+76460983 None:intergenic
GCAATGCATGTAGTAATGAA+CGG 0.659870 7:-76459076 MsaT038429.1:CDS
CCTCCACAATTTGAACACAT+GGG 0.660381 7:+76464572 None:intergenic
TTACCAGATGGACATAGCGA+CGG 0.660884 7:-76459007 MsaT038429.1:CDS
TGTGTAGCAATAAGTGCAGG+AGG 0.662801 7:-76460796 MsaT038429.1:intron
TATACCTGAGTGTTCTCTAG+CGG 0.665145 7:+76460942 None:intergenic
TGTCAATAAGAGAAGCAATG+AGG 0.665809 7:-76464598 MsaT038429.1:CDS
GCTATGTCCATCTGGTAACA+CGG 0.669438 7:+76459012 None:intergenic
ACTTGTGAATAGCCTTCCGA+CGG 0.670143 7:-76458915 MsaT038429.1:CDS
ATGATCCGGGTGAGCCACCG+GGG 0.670578 7:-76460630 MsaT038429.1:CDS
TACCTGAGTGTTCTCTAGCG+GGG 0.673914 7:+76460944 None:intergenic
TGGGACATACTCTCCAATGG+TGG 0.679385 7:-76460517 MsaT038429.1:CDS
TATGATGCAGGTGACATGGG+TGG 0.686232 7:-76461109 MsaT038429.1:intron
CAAACAAATATAGAAGCACA+AGG 0.687269 7:-76465356 MsaT038429.1:CDS
ATTGCCAAAGGACATGACCA+TGG 0.701545 7:-76460472 MsaT038429.1:CDS
GCACTAAGCACAATCCCCGG+TGG 0.713315 7:+76460616 None:intergenic
AGCTTCTTGCAATCACACAA+GGG 0.721070 7:+76463521 None:intergenic
AAACAAGCACAGACGCATCG+GGG 0.726013 7:-76459132 MsaT038429.1:CDS
AGGACGTTAGGGTAATGACA+AGG 0.727528 7:-76460663 MsaT038429.1:CDS
TGAAGCGGGTAGCCAAACTG+AGG 0.731803 7:+76460588 None:intergenic
CTCCCCGCTAGAGAACACTC+AGG 0.744703 7:-76460946 MsaT038429.1:intron
TCCGACGGCAAAGCTCACGG+TGG 0.748267 7:-76458900 MsaT038429.1:CDS
ACAAATATAGAAGCACAAGG+TGG 0.748500 7:-76465353 MsaT038429.1:CDS
AAAGTAGGAGTTGATAAACG+AGG 0.750797 7:+76465522 None:intergenic
ATGCATGTAGTAATGAACGG+CGG 0.775511 7:-76459073 MsaT038429.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 76458823 76465568 76458823 ID=MsaG038429
Chr7 mRNA 76458823 76465568 76458823 ID=MsaT038429.1;Parent=MsaG038429
Chr7 exon 76458823 76459194 76458823 ID=MsaT038429.1.exon9;Parent=MsaT038429.1
Chr7 CDS 76458823 76459194 76458823 ID=cds.MsaT038429.1;Parent=MsaT038429.1
Chr7 exon 76460444 76460811 76460444 ID=MsaT038429.1.exon8;Parent=MsaT038429.1
Chr7 CDS 76460444 76460811 76460444 ID=cds.MsaT038429.1;Parent=MsaT038429.1
Chr7 exon 76460947 76461121 76460947 ID=MsaT038429.1.exon7;Parent=MsaT038429.1
Chr7 CDS 76460947 76461121 76460947 ID=cds.MsaT038429.1;Parent=MsaT038429.1
Chr7 exon 76463098 76463313 76463098 ID=MsaT038429.1.exon6;Parent=MsaT038429.1
Chr7 CDS 76463098 76463313 76463098 ID=cds.MsaT038429.1;Parent=MsaT038429.1
Chr7 exon 76463462 76463563 76463462 ID=MsaT038429.1.exon5;Parent=MsaT038429.1
Chr7 CDS 76463462 76463563 76463462 ID=cds.MsaT038429.1;Parent=MsaT038429.1
Chr7 exon 76464025 76464687 76464025 ID=MsaT038429.1.exon4;Parent=MsaT038429.1
Chr7 CDS 76464025 76464687 76464025 ID=cds.MsaT038429.1;Parent=MsaT038429.1
Chr7 exon 76464933 76465050 76464933 ID=MsaT038429.1.exon3;Parent=MsaT038429.1
Chr7 CDS 76464933 76465050 76464933 ID=cds.MsaT038429.1;Parent=MsaT038429.1
Chr7 exon 76465146 76465378 76465146 ID=MsaT038429.1.exon2;Parent=MsaT038429.1
Chr7 CDS 76465146 76465378 76465146 ID=cds.MsaT038429.1;Parent=MsaT038429.1
Chr7 exon 76465479 76465568 76465479 ID=MsaT038429.1.exon1;Parent=MsaT038429.1
Chr7 CDS 76465479 76465568 76465479 ID=cds.MsaT038429.1;Parent=MsaT038429.1
Gene Sequence

>MsaT038429.1

ATGCCTTCTAGTGCAATCTCACAACCTCGTTTATCAACTCCTACTTTGGTTAAATCCATGTTCAACTCATCTGGTTTATCTTTAGCACTTCAAACAAATATAGAAGCACAAGGTGGTGATATGAAGAGATTAATGGCAGAAAATTTCCATCAAACAAATAATGGTTTGAGAAGGAATAGAGAAGATGAAGAACATGAAAGCTGCAGATCTGGTAGTGATAACATGGATGTTATTTCTGGTGATGATATTGATGCTGCCGACAATCCGCCAAGAAAAAAACGTTATCACCGACACACTCCTCAACAAATTCAAGAACTTGAATCGATGTTTAAAGAGTGTCCTCATCCTGATGAAAAACAAAGACTTGAACTTAGCAAAAGGCTTTGTTTGGAAACAAGACAAGTCAAGTTTTGGTTCCAAAATAGAAGAACACAAATGAAGACTCAATTGGAACGTCACGAGAATTCACTACTTAGGCAAGCAAATGATAAACTAAGAGCTGAGAACATGTCAATAAGAGAAGCAATGAGGAATCCCATGTGTTCAAATTGTGGAGGACCAGCAATTATTAGTGAAATTTCACTTGAAGAACAACATCTAAGAAATGAGAATGCAAGATTAAAAGATGAATTAGACCGAGTTTGTACACTCGCCGGCAAGTTTTTAGGCCGTCCAATTACGTCGTTACCAAACTCGAGTTTAGAAACTGGTTTTGTTGGTTTGAACAATACTTTGCCTTCAACAATGCCTTTAGGTCAAGATTTTGGCATGGTGTCAGTGTCACCACCTTCAATTACAAGAGGAACAAATATGGTAACAAATAGTGATAGTAATGGTTTTGATAGATCAATGGAAAGGTCTATGTTTCTTGAACTTGCTTTGGCTGCAATGGATGAGTTAGTGAAGATGGCACAAACTAATGAACCTCTTTGGATTAGCAATGTTGAAAATGGAAAAGAAATTCTTAATCATGAGGAATATACAAGGATTGTTAGTACTCCTTGTATTGGTTTGAAACCAAATGGTTTTGTTTCTGAGGCTTCTAGAGAAAGTGGTGTTGTTATCATAAACAGCTTGGCTCTTGTTGAAACTTTAATGGATTCAAATCGATGGTCAGAGATGTTCCCTTGTGTGATTGCAAGAAGCTCCACAACTGAAGTGATATCCAGTGGAATAAATGGAACCAGAAATGGTGCTCTACAACTGATGCAAGCTGAACTTCAAGTTCTTTCTCCTTTGGTTCCTGTTCGTGAGGTGAGTTTTCTAAGGTTTTGCAAGCAGCATGCAGAAGGGGTATGGGCAGTGGTTGATGTGTCCATAGATACCATCAGAGAAACTTCAGCTGGTGCACCCACTTTTCTTAACTGTAGGAGGCTTCCTTCTGGTTGTGTGGTTCAAGATATGCCAAATGGTTACTCTAAGGTGACATGGGTGGAGCATGCAGAATATGAGGAAAGCCAAGTTCACCAGCTCTATAGACCCTTGCTAAGCTTAGGCATGGGTTTTGGCGCACAACGTTGGGTTGCAACCCTCCAACGCCAATGTGAATGTCTAGCCATCTTAATGTCATCATCACTCCCCGCTAGAGAACACTCAGCAATAAGTGCAGGAGGAAGGCGTAGCATGCTAAAGCTAGCGCACCGAATGACAAACAACTTTTGTGCCGGAGTTTGTGCATCGACGGTGCACAAATGGAACAAATTGGATGCAGGGAATGTGGGCAAGGACGTTAGGGTAATGACAAGGAAGAGTGTGGATGATCCGGGTGAGCCACCGGGGATTGTGCTTAGTGCAGCAACCTCAGTTTGGCTACCCGCTTCACCACAAAAAGTCTTTGATTTCCTTCGCAACGAGAAGCTTAGAAGTGAATGGGACATACTCTCCAATGGTGGTCCAATGCAAGAGATGGCTCACATTGCCAAAGGACATGACCATGGCAACTGTGTCTCCCTCCTACGAGCTAGTGCTATTAATTCAAGTCAAAGTAGCATGTTGATTCTACAAGAAACAAGCACAGACGCATCGGGGTCGCTTGTTGTGTACGCGCCGGTAGATATTCCCGCAATGCATGTAGTAATGAACGGCGGCGATTCAGCTTATGTTGCGCTTCTTCCGTCGGGTTTCGCCGTGTTACCAGATGGACATAGCGACGGGAGTGGTAATCACGATGACGGGTCGCAGCGGAGAGCGAGTGGATCACTTTTGACGGTAGCATTCCAGATACTTGTGAATAGCCTTCCGACGGCAAAGCTCACGGTGGAATCAGTGGAAACGGTGAACAATCTCATTTCATGTACTATTCAAAAGATAAAAGTAGCACTTCAATGTGAGAGTTAA
Protein sequence

>MsaT038429.1

MPSSAISQPRLSTPTLVKSMFNSSGLSLALQTNIEAQGGDMKRLMAENFHQTNNGLRRNREDEEHESCRSGSDNMDVISGDDIDAADNPPRKKRYHRHTPQQIQELESMFKECPHPDEKQRLELSKRLCLETRQVKFWFQNRRTQMKTQLERHENSLLRQANDKLRAENMSIREAMRNPMCSNCGGPAIISEISLEEQHLRNENARLKDELDRVCTLAGKFLGRPITSLPNSSLETGFVGLNNTLPSTMPLGQDFGMVSVSPPSITRGTNMVTNSDSNGFDRSMERSMFLELALAAMDELVKMAQTNEPLWISNVENGKEILNHEEYTRIVSTPCIGLKPNGFVSEASRESGVVIINSLALVETLMDSNRWSEMFPCVIARSSTTEVISSGINGTRNGALQLMQAELQVLSPLVPVREVSFLRFCKQHAEGVWAVVDVSIDTIRETSAGAPTFLNCRRLPSGCVVQDMPNGYSKVTWVEHAEYEESQVHQLYRPLLSLGMGFGAQRWVATLQRQCECLAILMSSSLPAREHSAISAGGRRSMLKLAHRMTNNFCAGVCASTVHKWNKLDAGNVGKDVRVMTRKSVDDPGEPPGIVLSAATSVWLPASPQKVFDFLRNEKLRSEWDILSNGGPMQEMAHIAKGHDHGNCVSLLRASAINSSQSSMLILQETSTDASGSLVVYAPVDIPAMHVVMNGGDSAYVALLPSGFAVLPDGHSDGSGNHDDGSQRRASGSLLTVAFQILVNSLPTAKLTVESVETVNNLISCTIQKIKVALQCES*