AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038490 MtrunA17_Chr5g0411541 63.784 185 12 3 1 130 1 185 1.96e-59 186
MsaG038490 MtrunA17_Chr3g0141091 53.191 94 35 3 46 130 109 202 2.70e-15 71.2
MsaG038490 MtrunA17_Chr3g0131691 64.000 50 14 1 81 130 282 327 4.30e-12 62.4
MsaG038490 MtrunA17_Chr7g0252041 84.375 32 5 0 99 130 284 315 4.64e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038490 AT5G65640.2 75.472 53 11 1 80 130 155 207 6.27e-14 67.4
MsaG038490 AT5G65640.1 75.472 53 11 1 80 130 155 207 1.17e-13 67.0
MsaG038490 AT1G12860.3 78.947 38 8 0 99 136 265 302 4.64e-12 62.4
MsaG038490 AT3G26744.5 84.848 33 5 0 99 131 305 337 1.33e-11 61.2
MsaG038490 AT3G26744.1 84.375 32 5 0 99 130 305 336 5.38e-11 59.7
MsaG038490 AT3G26744.4 84.375 32 5 0 99 130 305 336 5.38e-11 59.7
MsaG038490 AT3G26744.2 84.375 32 5 0 99 130 305 336 5.38e-11 59.7
MsaG038490 AT3G26744.6 84.375 32 5 0 99 130 305 336 5.94e-11 59.3
MsaG038490 AT1G12860.1 84.375 32 5 0 99 130 265 296 5.98e-11 59.3
MsaG038490 AT1G12860.2 84.375 32 5 0 99 130 265 296 6.94e-11 59.3
MsaG038490 AT5G10570.1 79.070 43 9 0 88 130 138 180 8.41e-11 58.9

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCCGTCCTTCAACCCTTT+TGG 0.271608 7:-77083255 None:intergenic
AGTATAGAGTTTGGATTTGT+TGG 0.301074 7:+77083122 MsaT038490.1:CDS
AGTGACACCTTGCTGATCTT+AGG 0.318974 7:-77083353 None:intergenic
TGGAAGTGAGAACAAGAGTT+TGG 0.348911 7:+77083142 MsaT038490.1:CDS
CTTTCTTGCTGTGGTAAAAC+TGG 0.352353 7:-77083101 None:intergenic
CAGCAAGAAAGTATAGAGTT+TGG 0.356994 7:+77083113 MsaT038490.1:CDS
AATGAGTTAATACTCCCTAA+TGG 0.401034 7:+77082907 MsaT038490.1:CDS
ACGGCCTTCAAAGAATCTTA+TGG 0.423564 7:+77083271 MsaT038490.1:CDS
GAAGAAAGCGTTTGAATGAC+AGG 0.430541 7:+77083306 MsaT038490.1:CDS
AGTCAAAGGTCCAACCATTA+GGG 0.456761 7:-77082921 None:intergenic
TCAGCCATAAGATTCTTTGA+AGG 0.463412 7:-77083275 None:intergenic
GTGACACCTTGCTGATCTTA+GGG 0.466467 7:-77083352 None:intergenic
AAAATGGAACTTTCTCAACT+TGG 0.469304 7:+77082829 None:intergenic
AGTTTGGAACAAAGTAAAAT+CGG 0.479256 7:+77083158 MsaT038490.1:CDS
ATGACAGGCTTTCCATGCTT+AGG 0.479470 7:+77083321 MsaT038490.1:CDS
GCCAAATCCAAAAGGGTTGA+AGG 0.489270 7:+77083248 MsaT038490.1:CDS
CACAAACCCATGTTAAAGAC+TGG 0.505791 7:-77083212 None:intergenic
TATTAACTCATTCAAAACAT+TGG 0.513404 7:-77082896 None:intergenic
CAAAGAATCTTATGGCTGAA+AGG 0.515993 7:+77083279 MsaT038490.1:CDS
GAGACTCCAGTCTTTAACAT+GGG 0.538936 7:+77083206 MsaT038490.1:CDS
AACTCTATACTTTCTTGCTG+TGG 0.547442 7:-77083110 None:intergenic
TTGATGATTCTCAGTTCCTG+AGG 0.556181 7:+77083064 MsaT038490.1:intron
AAGATCAGCAAGGTGTCACT+AGG 0.558295 7:+77083356 MsaT038490.1:CDS
GGTGCTGAATCATCAACCTC+AGG 0.571254 7:-77083080 None:intergenic
TAAAATCGGCTGCAAAGTTG+AGG 0.590576 7:+77083172 MsaT038490.1:CDS
TGAGACTCCAGTCTTTAACA+TGG 0.608396 7:+77083205 MsaT038490.1:CDS
AATCCAAAAGGGTTGAAGGA+CGG 0.613450 7:+77083252 MsaT038490.1:CDS
AGGGACAATTGACCTAAGCA+TGG 0.627542 7:-77083333 None:intergenic
AATTGTCCCTAAGATCAGCA+AGG 0.709185 7:+77083346 MsaT038490.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 77082832 77083409 77082832 ID=MsaG038490
Chr7 mRNA 77082832 77083409 77082832 ID=MsaT038490.1;Parent=MsaG038490
Chr7 exon 77082832 77082928 77082832 ID=MsaT038490.1.exon1;Parent=MsaT038490.1
Chr7 CDS 77082832 77082928 77082832 ID=cds.MsaT038490.1;Parent=MsaT038490.1
Chr7 exon 77083078 77083409 77083078 ID=MsaT038490.1.exon2;Parent=MsaT038490.1
Chr7 CDS 77083078 77083409 77083078 ID=cds.MsaT038490.1;Parent=MsaT038490.1
Gene Sequence

>MsaT038490.1

ATGGAACTTTCTCAACTTGGTTTTCTTGAGGAGCTACTAGCTCCAAGAAAAGACACATGTTTATCCAATGTTTTGAATGAGTTAATACTCCCTAATGTTCCTGAGGTTGATGATTCAGCACCAGTTTTACCACAGCAAGAAAGTATAGAGTTTGGATTTGTTGGAAGTGAGAACAAGAGTTTGGAACAAAGTAAAATCGGCTGCAAAGTTGAGGAACAAGTTAGTGAGACTCCAGTCTTTAACATGGGTTTGTGTGATGAGAAAAAAGCCAAATCCAAAAGGGTTGAAGGACGGCCTTCAAAGAATCTTATGGCTGAAAGGAGAAGAAGAAAGCGTTTGAATGACAGGCTTTCCATGCTTAGGTCAATTGTCCCTAAGATCAGCAAGGTGTCACTAGGCCAATTGCCAAATAAAATATATATAGTTTGA

Protein sequence

>MsaT038490.1

MELSQLGFLEELLAPRKDTCLSNVLNELILPNVPEVDDSAPVLPQQESIEFGFVGSENKSLEQSKIGCKVEEQVSETPVFNMGLCDEKKAKSKRVEGRPSKNLMAERRRRKRLNDRLSMLRSIVPKISKVSLGQLPNKIYIV*