AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038527

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038527 MtrunA17_Chr7g0247631 98.632 658 9 0 1 658 45 702 0.0 1358
MsaG038527 MtrunA17_Chr5g0408851 51.307 612 274 7 48 656 60 650 0.0 636
MsaG038527 MtrunA17_Chr4g0023601 48.630 438 215 4 220 651 12 445 2.64e-148 439
MsaG038527 MtrunA17_Chr2g0323051 47.672 451 224 6 206 650 73 517 6.00e-142 426
MsaG038527 MtrunA17_Chr5g0400001 45.815 454 229 6 199 648 65 505 2.06e-126 384
MsaG038527 MtrunA17_Chr5g0399991 46.591 440 218 6 221 656 89 515 7.49e-124 378
MsaG038527 MtrunA17_Chr4g0055281 46.292 445 228 7 208 648 65 502 7.72e-123 375
MsaG038527 MtrunA17_Chr6g0481031 44.954 436 227 8 221 648 106 536 8.16e-122 374
MsaG038527 MtrunA17_Chr8g0388651 37.444 446 263 9 211 648 72 509 9.87e-89 286
MsaG038527 MtrunA17_Chr2g0286431 36.866 434 255 8 221 649 83 502 7.28e-87 281
MsaG038527 MtrunA17_Chr1g0154651 47.253 91 45 1 37 127 13 100 2.54e-15 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038527 AT2G45880.1 66.972 654 206 5 5 655 36 682 0.0 919
MsaG038527 AT4G00490.1 69.762 463 136 1 194 656 72 530 0.0 711
MsaG038527 AT5G45300.1 51.570 605 275 7 48 651 96 683 0.0 608
MsaG038527 AT5G45300.2 51.570 605 273 7 48 651 96 681 0.0 608
MsaG038527 AT5G45300.3 51.570 605 275 7 48 651 110 697 0.0 608
MsaG038527 AT2G32290.1 48.085 470 229 6 192 650 43 508 2.21e-142 427
MsaG038527 AT4G15210.1 46.697 439 223 6 220 651 13 447 2.93e-140 419
MsaG038527 AT4G17090.1 48.148 432 213 6 221 648 86 510 1.13e-133 404
MsaG038527 AT4G15210.2 48.101 395 198 4 220 611 13 403 1.33e-131 394
MsaG038527 AT3G23920.1 45.767 437 224 7 221 648 106 538 4.74e-127 387
MsaG038527 AT4G15210.3 45.477 398 207 5 260 651 1 394 1.41e-123 374
MsaG038527 AT4G15210.4 45.477 398 207 5 260 651 1 394 1.41e-123 374
MsaG038527 AT5G55700.1 38.479 447 251 9 221 651 92 530 7.98e-96 305
MsaG038527 AT5G18670.1 34.803 431 262 8 221 648 87 501 1.15e-68 233
MsaG038527 AT5G55700.2 38.304 342 191 8 322 651 34 367 1.20e-68 228

Find 204 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACGAATGTGGAGGCAATTT+TGG 0.132573 7:-77368375 MsaT038527.1:CDS
AAGAAAGAGGGCACACTATT+TGG 0.160450 7:-77367051 MsaT038527.1:CDS
AAATACCTGCTTCTCATTAA+TGG 0.165257 7:+77369784 None:intergenic
CCTTCCATCTGGATCATTAA+TGG 0.228989 7:+77365286 None:intergenic
CCCCATTGGATTGCGGAAAT+TGG 0.229009 7:-77368330 MsaT038527.1:CDS
TCCCTCAAGGGAGTTGCTTC+TGG 0.230220 7:-77369971 MsaT038527.1:CDS
TACAGATATCAGGTATTTAT+TGG 0.240649 7:-77365804 MsaT038527.1:intron
GCAATTATAATCTTAGAGTT+AGG 0.256212 7:-77370720 MsaT038527.1:CDS
ATGCTCCTCAAGAGTATAAT+TGG 0.266684 7:-77368561 MsaT038527.1:CDS
TATCTCCAACCATGCTTTAC+AGG 0.289028 7:+77367688 None:intergenic
AAAATCACGTTCTGGTAATC+TGG 0.293408 7:+77368987 None:intergenic
TTGATTTCATGAGAAGTTTC+CGG 0.295099 7:-77367801 MsaT038527.1:CDS
AGTTAGCTATAACAAGGTTT+TGG 0.306669 7:-77365321 MsaT038527.1:CDS
AGAAGCTGTTCTTGATCTTC+AGG 0.313620 7:-77364774 MsaT038527.1:CDS
CGTTCAAACTCTATGAAGTT+TGG 0.314971 7:+77365212 None:intergenic
ATTTATTATATACAGATATC+AGG 0.333139 7:-77365814 MsaT038527.1:intron
AGTATATGTTGAAAAGTCTT+AGG 0.334105 7:-77367084 MsaT038527.1:CDS
GGTGATTATGATGGCTTCTA+TGG 0.335535 7:-77366959 MsaT038527.1:CDS
CTTCCATCTGGATCATTAAT+GGG 0.337375 7:+77365287 None:intergenic
GACAATCCTCCCTTGATTGA+TGG 0.338381 7:-77369818 MsaT038527.1:CDS
TTGTAGGTACCCACATTATC+TGG 0.340452 7:+77367019 None:intergenic
TGTTATAGCTAACTCTGTTT+AGG 0.347312 7:+77365330 None:intergenic
TCCTGGTATTGGTGAGTTCC+AGG 0.351906 7:-77367665 MsaT038527.1:intron
GGATCTTGGCAGGGCTGCGC+AGG 0.352776 7:-77370747 MsaT038527.1:CDS
TGAGCTTAAGCTTAAGTTGC+AGG 0.359918 7:-77368506 MsaT038527.1:intron
AAGGGAGTTGCTTCTGGCTA+TGG 0.363095 7:-77369965 MsaT038527.1:CDS
GGTGGGAGTGGGATGGGAAC+TGG 0.366608 7:-77370913 MsaT038527.1:CDS
ATCTGGATCATTAATGGGCT+TGG 0.366707 7:+77365292 None:intergenic
ATTCCCCAGGAAAGACATTC+CGG 0.367856 7:+77368258 None:intergenic
ATCACATAAACAGGAACATA+TGG 0.371428 7:+77368955 None:intergenic
AGAAAGAGGGCACACTATTT+GGG 0.383259 7:-77367050 MsaT038527.1:CDS
TTCCCGATCTCAAACATCTA+TGG 0.384478 7:-77369864 MsaT038527.1:CDS
TGACTGCTGGTGGGGAATAG+TGG 0.395519 7:-77368590 MsaT038527.1:CDS
ACCAATTTCCGCAATCCAAT+GGG 0.401881 7:+77368329 None:intergenic
TCGGGATCAGCAAATGGTTC+TGG 0.406421 7:+77365619 None:intergenic
CTGATCCCGAGCGATTAGTT+TGG 0.407402 7:-77365606 MsaT038527.1:CDS
ACAACCCGGAATGTCTTTCC+TGG 0.411796 7:-77368263 MsaT038527.1:CDS
ACCGGTAGCATCACATAAAC+AGG 0.415437 7:+77368946 None:intergenic
GTTCTCTCTTTCTCTTCTAC+CGG 0.419911 7:+77370808 None:intergenic
GACATGCATTATAGTCTAAA+GGG 0.422702 7:+77369939 None:intergenic
TCGGGGATGGTAGAGATTGA+TGG 0.429329 7:-77370934 None:intergenic
CTTTAGTTGATCATGGAAAC+CGG 0.430369 7:-77366907 MsaT038527.1:CDS
GATGAAAAGTGCCTTCCATC+TGG 0.431328 7:+77365275 None:intergenic
GGTATGAAAACATCTTTCAT+TGG 0.431483 7:+77369917 None:intergenic
AGCAAATGTTGATGGTGTTA+TGG 0.432333 7:-77368614 MsaT038527.1:CDS
TCTCAGGCTTTAGTTGATCA+TGG 0.433442 7:-77366914 MsaT038527.1:CDS
GGCTATCACTGCAAGGATCT+TGG 0.439690 7:-77370761 MsaT038527.1:CDS
TCAGAAGTGGACTGAGCCTC+AGG 0.439950 7:+77365243 None:intergenic
ATTATGATGGCTTCTATGGC+AGG 0.443004 7:-77366955 MsaT038527.1:CDS
AATACTCATTAAACTCTATC+CGG 0.443205 7:+77367782 None:intergenic
AGTGGGATGGGAACTGGAGC+CGG 0.444427 7:-77370907 MsaT038527.1:CDS
TTGGCAGGGCTGCGCAGGCA+TGG 0.447154 7:-77370742 MsaT038527.1:CDS
GAAGTTGAGGACAGATCATA+TGG 0.448226 7:+77369884 None:intergenic
ATTGATGGTGGGAGTGGGAT+GGG 0.452488 7:-77370919 MsaT038527.1:CDS
AATTCTCAGCCACACGAAAC+GGG 0.456914 7:-77366998 MsaT038527.1:CDS
GTGTTGCAGGTGTTGAATGC+CGG 0.458021 7:-77365398 MsaT038527.1:intron
CTCCCAGCAAAATCACGTTC+TGG 0.459187 7:+77368979 None:intergenic
CAATTCTCAGCCACACGAAA+CGG 0.459319 7:-77366999 MsaT038527.1:CDS
TTTGTATTCCATTACCCCAT+TGG 0.460459 7:-77368344 MsaT038527.1:CDS
GGACATGCATTATAGTCTAA+AGG 0.460742 7:+77369938 None:intergenic
CAATTATAATCTTAGAGTTA+GGG 0.460826 7:-77370719 MsaT038527.1:CDS
GAAGGAAAAGTAGTTCCATC+TGG 0.463042 7:+77370640 None:intergenic
TTGAATGCCGGGTGGGATGT+TGG 0.467187 7:-77365386 MsaT038527.1:CDS
CACCATAGATGTTTGAGATC+GGG 0.467798 7:+77369862 None:intergenic
ATCTATTGAACCATCAATCA+AGG 0.468065 7:+77369808 None:intergenic
CAAGAGCAAGCAGGCACCCC+TGG 0.468199 7:-77370853 MsaT038527.1:CDS
ATCACTGCAAGGATCTTGGC+AGG 0.470853 7:-77370757 MsaT038527.1:CDS
TTCGTGTGGCTGAGAATTGT+AGG 0.472590 7:+77367003 None:intergenic
GAACTGGAGCCGGTGACAAC+AGG 0.472912 7:-77370897 MsaT038527.1:CDS
TTCATCTCCATGATTGAAGT+TGG 0.474972 7:-77367748 MsaT038527.1:CDS
GCCAGAAGCAACTCCCTTGA+GGG 0.477029 7:+77369970 None:intergenic
CAAGCAGGCACCCCTGGTGG+AGG 0.477433 7:-77370847 MsaT038527.1:CDS
TCATACCTTTGCAGCAATGC+AGG 0.480344 7:+77366850 None:intergenic
AGGGAGTTGCTTCTGGCTAT+GGG 0.481665 7:-77369964 MsaT038527.1:CDS
AAATCACGTTCTGGTAATCT+GGG 0.482509 7:+77368988 None:intergenic
TCCGCAATCCAATGGGGTAA+TGG 0.483794 7:+77368336 None:intergenic
AGGAAAGCAGCAGAAGAAAG+AGG 0.484099 7:-77367064 MsaT038527.1:CDS
CTCTAGCAAGGGAAGCTGGA+TGG 0.485605 7:-77370672 MsaT038527.1:CDS
CAAAGCTAAGAGAGAGGCGC+CGG 0.488485 7:-77370786 MsaT038527.1:CDS
TTCCCCAGGAAAGACATTCC+GGG 0.492422 7:+77368259 None:intergenic
GATCTTCGATTGCCTCCACC+AGG 0.493863 7:+77370835 None:intergenic
GCTGCTCTAGCAAGGGAAGC+TGG 0.494031 7:-77370676 MsaT038527.1:CDS
GTAGAGATTGATGGTGGGAG+TGG 0.497271 7:-77370925 MsaT038527.1:CDS
GTGTTATGGTTGACTGCTGG+TGG 0.497598 7:-77368600 MsaT038527.1:CDS
AGCTAAGAGAGAGGCGCCGG+AGG 0.498212 7:-77370783 MsaT038527.1:CDS
TAAATGTGAGCTTGTTGATC+CGG 0.500589 7:-77368671 MsaT038527.1:CDS
TGGAAACCGGGTACTTTCAA+TGG 0.501041 7:-77366894 MsaT038527.1:CDS
ACCTAGTCCAACTTCAATCA+TGG 0.503665 7:+77367741 None:intergenic
AAGCATGGTTGGAGATATCC+TGG 0.503866 7:-77367682 MsaT038527.1:CDS
CCTCCATCTGTTTCCCTCAA+GGG 0.504458 7:-77369983 MsaT038527.1:CDS
GATTGATGGTGGGAGTGGGA+TGG 0.506994 7:-77370920 MsaT038527.1:CDS
TACCAATTTCCGCAATCCAA+TGG 0.510010 7:+77368328 None:intergenic
ATGGTGTTATGGTTGACTGC+TGG 0.511687 7:-77368603 MsaT038527.1:CDS
TTGAAATCAGCAAATGTTGA+TGG 0.518512 7:-77368622 MsaT038527.1:CDS
AATCGCTCGGGATCAGCAAA+TGG 0.518578 7:+77365613 None:intergenic
GGCTAGAGGGCCAGATAATG+TGG 0.519736 7:-77367029 MsaT038527.1:CDS
GTTATGGTTGACTGCTGGTG+GGG 0.522259 7:-77368598 MsaT038527.1:CDS
CAACCCGGAATGTCTTTCCT+GGG 0.523953 7:-77368262 MsaT038527.1:CDS
GCCTCCATCTGTTTCCCTCA+AGG 0.524523 7:-77369984 MsaT038527.1:CDS
AGATACCATTAATGAGAAGC+AGG 0.524558 7:-77369789 MsaT038527.1:intron
CTTGTCCTGTAAAGCATGGT+TGG 0.525812 7:-77367693 MsaT038527.1:CDS
GAGGCTCAGTCCACTTCTGA+TGG 0.525869 7:-77365240 MsaT038527.1:CDS
TTACAAGAGACTCTTTCAAA+TGG 0.526474 7:-77368533 MsaT038527.1:CDS
GGCACACTATTTGGGCTAGA+GGG 0.530326 7:-77367042 MsaT038527.1:CDS
GGTTGGAGATATCCTGGTAT+TGG 0.531486 7:-77367676 MsaT038527.1:CDS
TGTTATGGTTGACTGCTGGT+GGG 0.531507 7:-77368599 MsaT038527.1:CDS
TCTAGCAAGGGAAGCTGGAT+GGG 0.532361 7:-77370671 MsaT038527.1:CDS
GGGATCCTGCATTGCTGCAA+AGG 0.534136 7:-77366855 MsaT038527.1:intron
AGTTTGAACGATTTGTCAAG+AGG 0.534218 7:-77365199 MsaT038527.1:CDS
CCACCATAGATGTTTGAGAT+CGG 0.535969 7:+77369861 None:intergenic
AACTTGCCAAACTAATCGCT+CGG 0.538712 7:+77365600 None:intergenic
GTTGCATGGATTATAATATC+CGG 0.541192 7:+77365744 None:intergenic
CCATGCTTTACAGGACAAGA+TGG 0.541344 7:+77367697 None:intergenic
ACAGCAATGTTAAAGAGAAA+CGG 0.542903 7:-77365700 MsaT038527.1:CDS
TCCATTACCCCATTGGATTG+CGG 0.544817 7:-77368337 MsaT038527.1:CDS
ATCTCAAACATCTATGGTGG+GGG 0.545777 7:-77369858 MsaT038527.1:CDS
ACGTTCCTTATCTATTCCCC+AGG 0.549619 7:+77368245 None:intergenic
GGATGGGTGGTTCTTCCAGA+TGG 0.550123 7:-77370655 MsaT038527.1:CDS
TCTTTCCTGGGGAATAGATA+AGG 0.553960 7:-77368250 MsaT038527.1:CDS
TGTTGCAGGTGTTGAATGCC+GGG 0.554149 7:-77365397 MsaT038527.1:intron
GAGCAAGCAGGCACCCCTGG+TGG 0.554316 7:-77370850 MsaT038527.1:CDS
CCTCAAGAGTATAATTGGAA+TGG 0.554372 7:-77368556 MsaT038527.1:CDS
ATGTCATTGCTGCTCTAGCA+AGG 0.555742 7:-77370684 MsaT038527.1:CDS
ACAGATGGAGGCTGTTGAGA+AGG 0.557652 7:+77369995 None:intergenic
TGTTTGAGATCGGGAAGTTG+AGG 0.559441 7:+77369871 None:intergenic
CCATTCCAATTATACTCTTG+AGG 0.560764 7:+77368556 None:intergenic
GCATAGCCATCACGGTTGCA+TGG 0.562409 7:+77365730 None:intergenic
CATGCTGCTGAATTAGCTGC+CGG 0.562553 7:-77365763 MsaT038527.1:CDS
TTTGGTCGTTCCATCAGAAG+TGG 0.562705 7:+77365230 None:intergenic
CGATCTCAAACATCTATGGT+GGG 0.562927 7:-77369860 MsaT038527.1:CDS
CAAAAGAAACCCGTTTCGTG+TGG 0.568671 7:+77366989 None:intergenic
TCACATAAACAGGAACATAT+GGG 0.570006 7:+77368956 None:intergenic
AGCTGCTTTAGTATACCATC+CGG 0.570265 7:+77368652 None:intergenic
TCCTTGCAGTGATAGCCCTC+CGG 0.572338 7:+77370767 None:intergenic
ATTGGATTGCGGAAATTGGT+AGG 0.573552 7:-77368326 MsaT038527.1:CDS
CCGATCTCAAACATCTATGG+TGG 0.574081 7:-77369861 MsaT038527.1:CDS
GGGATGGTAGAGATTGATGG+TGG 0.574890 7:-77370931 MsaT038527.1:CDS
AGATATCAGGTATTTATTGG+TGG 0.576841 7:-77365801 MsaT038527.1:CDS
ACTTGCCAAACTAATCGCTC+GGG 0.578201 7:+77365601 None:intergenic
TTACAGGGACAGATGCCAGC+TGG 0.579767 7:-77370058 MsaT038527.1:intron
TAGCTAACTCTGTTTAGGCA+AGG 0.580773 7:+77365335 None:intergenic
ACCTGGAACTCACCAATACC+AGG 0.580960 7:+77367664 None:intergenic
AAGCCCATTAATGATCCAGA+TGG 0.584215 7:-77365290 MsaT038527.1:CDS
CTGCTTGCTCTTGAAACTCG+TGG 0.584946 7:+77370863 None:intergenic
TAGAGATTGATGGTGGGAGT+GGG 0.585198 7:-77370924 MsaT038527.1:CDS
ACTGAGCCTCAGGTATGTAA+AGG 0.587637 7:+77365253 None:intergenic
GAGGCTGTTGAGAAGGCACA+TGG 0.587814 7:+77370002 None:intergenic
TTGCTGAAACCTGTTGTCAC+CGG 0.588256 7:+77370888 None:intergenic
TCCTGTTTATGTGATGCTAC+CGG 0.588479 7:-77368947 MsaT038527.1:intron
CGATTTGTCAAGAGGATGCA+CGG 0.590836 7:-77365191 MsaT038527.1:intron
AGATCATATGGAGATGGAGA+TGG 0.591075 7:+77369896 None:intergenic
GGAAAGCAGCAGAAGAAAGA+GGG 0.591875 7:-77367063 MsaT038527.1:CDS
GGGCACACTATTTGGGCTAG+AGG 0.593100 7:-77367043 MsaT038527.1:CDS
GAGGACAGATCATATGGAGA+TGG 0.593231 7:+77369890 None:intergenic
TTTCATCCTTTACATACCTG+AGG 0.594089 7:-77365259 MsaT038527.1:CDS
GAGAGGTCGGACTGCTGTGG+AGG 0.594354 7:-77368217 MsaT038527.1:intron
ATCACGTTCTGGTAATCTGG+GGG 0.596266 7:+77368990 None:intergenic
ATGGTATACTAAAGCAGCTG+AGG 0.597687 7:-77368648 MsaT038527.1:CDS
GCTAAGAGAGAGGCGCCGGA+GGG 0.598262 7:-77370782 MsaT038527.1:CDS
CACGAGTTTCAAGAGCAAGC+AGG 0.600604 7:-77370862 MsaT038527.1:CDS
AGCCAGAAGCAACTCCCTTG+AGG 0.601428 7:+77369969 None:intergenic
ACTGCAGAATTACCACCAGC+TGG 0.602253 7:+77370043 None:intergenic
GATCTCAAACATCTATGGTG+GGG 0.602792 7:-77369859 MsaT038527.1:CDS
CCATCTTGTCCTGTAAAGCA+TGG 0.604293 7:-77367697 MsaT038527.1:CDS
TATAATCCATGCAACCGTGA+TGG 0.604547 7:-77365736 MsaT038527.1:CDS
AGCAAGGGAAGCTGGATGGG+TGG 0.608234 7:-77370668 MsaT038527.1:CDS
CATGCTTTACAGGACAAGAT+GGG 0.612017 7:+77367698 None:intergenic
TCTTCGATTGCCTCCACCAG+GGG 0.616021 7:+77370837 None:intergenic
GCCGGAGGGCTATCACTGCA+AGG 0.616217 7:-77370768 MsaT038527.1:CDS
AGTGCATTCTCACTAACGAC+GGG 0.621609 7:+77365359 None:intergenic
AGAGAACAAAGCTAAGAGAG+AGG 0.624197 7:-77370792 MsaT038527.1:CDS
AGGCAATCGAAGATCTAGAC+CGG 0.625299 7:-77370827 MsaT038527.1:CDS
ATCTTCGATTGCCTCCACCA+GGG 0.625792 7:+77370836 None:intergenic
GCAGGTGTTGAATGCCGGGT+GGG 0.628049 7:-77365393 MsaT038527.1:intron
TGATTGCAGCATAGCCATCA+CGG 0.628887 7:+77365722 None:intergenic
TTTAGTTGATCATGGAAACC+GGG 0.629705 7:-77366906 MsaT038527.1:CDS
CCATTAATGATCCAGATGGA+AGG 0.631548 7:-77365286 MsaT038527.1:CDS
TCCATGATTGAAGTTGGACT+AGG 0.633760 7:-77367742 MsaT038527.1:CDS
CTGCTGGTGGGGAATAGTGG+AGG 0.638557 7:-77368587 MsaT038527.1:CDS
TAGAGAGGGAAGACACAACC+CGG 0.640033 7:-77368277 MsaT038527.1:CDS
ACTCCCTTGAGGGAAACAGA+TGG 0.640870 7:+77369980 None:intergenic
TCTATTGAACCATCAATCAA+GGG 0.641869 7:+77369809 None:intergenic
AAACAGAGTTAGCTATAACA+AGG 0.642740 7:-77365327 MsaT038527.1:CDS
TGTCATTGCTGCTCTAGCAA+GGG 0.650040 7:-77370683 MsaT038527.1:CDS
AATCACGTTCTGGTAATCTG+GGG 0.653035 7:+77368989 None:intergenic
TGTGAGCTTGTTGATCCGGA+TGG 0.654767 7:-77368667 MsaT038527.1:CDS
TGGTATACTAAAGCAGCTGA+GGG 0.655208 7:-77368647 MsaT038527.1:CDS
GGGTAGTCCAACATCCCACC+CGG 0.657727 7:+77365379 None:intergenic
GGATGGTAGAGATTGATGGT+GGG 0.658760 7:-77370930 MsaT038527.1:CDS
TCACTGCAAGGATCTTGGCA+GGG 0.658891 7:-77370756 MsaT038527.1:CDS
CCCTTGAGGGAAACAGATGG+AGG 0.658899 7:+77369983 None:intergenic
TGCAGGTGTTGAATGCCGGG+TGG 0.661840 7:-77365394 MsaT038527.1:intron
TTTGAGAGGTCGGACTGCTG+TGG 0.661975 7:-77368220 MsaT038527.1:CDS
GAATGTGTGCAAATCAACAC+AGG 0.666849 7:+77365660 None:intergenic
AAGTGCATTCTCACTAACGA+CGG 0.670203 7:+77365358 None:intergenic
CAGGGACAGATGCCAGCTGG+TGG 0.672850 7:-77370055 MsaT038527.1:CDS
AACCCGGAATGTCTTTCCTG+GGG 0.677027 7:-77368261 MsaT038527.1:CDS
GGGTATCTTAGCTCTCCACA+TGG 0.681100 7:+77367718 None:intergenic
ATTGAACCATCAATCAAGGG+AGG 0.685955 7:+77369812 None:intergenic
GCTAGAGGGCCAGATAATGT+GGG 0.688613 7:-77367028 MsaT038527.1:CDS
TCACGTTCTGGTAATCTGGG+GGG 0.695800 7:+77368991 None:intergenic
GAAGTTGGACTAGGTCCATG+TGG 0.708297 7:-77367733 MsaT038527.1:CDS
CCAATTTCCGCAATCCAATG+GGG 0.711257 7:+77368330 None:intergenic
AGGCTGTTGAGAAGGCACAT+GGG 0.748056 7:+77370003 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 77364769 77370950 77364769 ID=MsaG038527
Chr7 mRNA 77364769 77370950 77364769 ID=MsaT038527.1;Parent=MsaG038527
Chr7 exon 77364769 77364797 77364769 ID=MsaT038527.1.exon10;Parent=MsaT038527.1
Chr7 CDS 77364769 77364797 77364769 ID=cds.MsaT038527.1;Parent=MsaT038527.1
Chr7 exon 77365192 77365411 77365192 ID=MsaT038527.1.exon9;Parent=MsaT038527.1
Chr7 CDS 77365192 77365411 77365192 ID=cds.MsaT038527.1;Parent=MsaT038527.1
Chr7 exon 77365603 77365821 77365603 ID=MsaT038527.1.exon8;Parent=MsaT038527.1
Chr7 CDS 77365603 77365821 77365603 ID=cds.MsaT038527.1;Parent=MsaT038527.1
Chr7 exon 77366856 77367116 77366856 ID=MsaT038527.1.exon7;Parent=MsaT038527.1
Chr7 CDS 77366856 77367116 77366856 ID=cds.MsaT038527.1;Parent=MsaT038527.1
Chr7 exon 77367666 77367830 77367666 ID=MsaT038527.1.exon6;Parent=MsaT038527.1
Chr7 CDS 77367666 77367830 77367666 ID=cds.MsaT038527.1;Parent=MsaT038527.1
Chr7 exon 77368218 77368412 77368218 ID=MsaT038527.1.exon5;Parent=MsaT038527.1
Chr7 CDS 77368218 77368412 77368218 ID=cds.MsaT038527.1;Parent=MsaT038527.1
Chr7 exon 77368507 77368710 77368507 ID=MsaT038527.1.exon4;Parent=MsaT038527.1
Chr7 CDS 77368507 77368710 77368507 ID=cds.MsaT038527.1;Parent=MsaT038527.1
Chr7 exon 77368948 77369025 77368948 ID=MsaT038527.1.exon3;Parent=MsaT038527.1
Chr7 CDS 77368948 77369025 77368948 ID=cds.MsaT038527.1;Parent=MsaT038527.1
Chr7 exon 77369790 77370074 77369790 ID=MsaT038527.1.exon2;Parent=MsaT038527.1
Chr7 CDS 77369790 77370074 77369790 ID=cds.MsaT038527.1;Parent=MsaT038527.1
Chr7 exon 77370630 77370950 77370630 ID=MsaT038527.1.exon1;Parent=MsaT038527.1
Chr7 CDS 77370630 77370950 77370630 ID=cds.MsaT038527.1;Parent=MsaT038527.1
Gene Sequence

>MsaT038527.1

ATGGTAGAGATTGATGGTGGGAGTGGGATGGGAACTGGAGCCGGTGACAACAGGTTTCAGCAACACCACGAGTTTCAAGAGCAAGCAGGCACCCCTGGTGGAGGCAATCGAAGATCTAGACCGGTAGAAGAGAAAGAGAGAACAAAGCTAAGAGAGAGGCGCCGGAGGGCTATCACTGCAAGGATCTTGGCAGGGCTGCGCAGGCATGGCAATTATAATCTTAGAGTTAGGGCTGACATTAATGATGTCATTGCTGCTCTAGCAAGGGAAGCTGGATGGGTGGTTCTTCCAGATGGAACTACTTTTCCTTCAAAATCTCAGGGACAGATGCCAGCTGGTGGTAATTCTGCAGTTGTTACTTCATCATCATCCCATGTGCCTTCTCAACAGCCTCCATCTGTTTCCCTCAAGGGAGTTGCTTCTGGCTATGGGAGCCCTTTAGACTATAATGCATGTCCAATGAAAGATGTTTTCATACCATCTCCATCTCCATATGATCTGTCCTCAACTTCCCGATCTCAAACATCTATGGTGGGGGATGGGGAAGCGCAAAGAGACAATCCTCCCTTGATTGATGGTTCAATAGATACCATTAATGAGAAGCAGATTGCTGACATTCCCCCCAGATTACCAGAACGTGATTTTGCTGGGAGCCCATATGTTCCTGTTTATGTGATGCTACCGTTGGGGGTGATCAATATTAAATGTGAGCTTGTTGATCCGGATGGTATACTAAAGCAGCTGAGGGTATTGAAATCAGCAAATGTTGATGGTGTTATGGTTGACTGCTGGTGGGGAATAGTGGAGGCACATGCTCCTCAAGAGTATAATTGGAATGGTTACAAGAGACTCTTTCAAATGGTGCGTGAGCTTAAGCTTAAGTTGCAGGTTGTCATGTCTTTTCACGAATGTGGAGGCAATTTTGGTGATGATGTTTGTATTCCATTACCCCATTGGATTGCGGAAATTGGTAGGAGCAATCCAGATATTTTTTTCACTGATAGAGAGGGAAGACACAACCCGGAATGTCTTTCCTGGGGAATAGATAAGGAACGTGTTTTGAGAGGTCGGACTGCTGTGGAGGTTTATTTTGATTTCATGAGAAGTTTCCGGATAGAGTTTAATGAGTATTTTGAGGATGGCTTCATCTCCATGATTGAAGTTGGACTAGGTCCATGTGGAGAGCTAAGATACCCATCTTGTCCTGTAAAGCATGGTTGGAGATATCCTGGTATTGGTGAGTTCCAGTGCTATGATCAGTATATGTTGAAAAGTCTTAGGAAAGCAGCAGAAGAAAGAGGGCACACTATTTGGGCTAGAGGGCCAGATAATGTGGGTACCTACAATTCTCAGCCACACGAAACGGGTTTCTTTTGCGATGGGGGTGATTATGATGGCTTCTATGGCAGGTTTTTTCTCAACTGGTACTCTCAGGCTTTAGTTGATCATGGAAACCGGGTACTTTCAATGGCAAAATTAGCTTTTGAGGGATCCTGCATTGCTGCAAAGATATCAGGTATTTATTGGTGGTACAAGACTGCTAGTCATGCTGCTGAATTAGCTGCCGGATATTATAATCCATGCAACCGTGATGGCTATGCTGCAATCACAGCAATGTTAAAGAGAAACGGTGTTAACTTAAACATTGCCTGTGTTGATTTGCACACATTCAATCAACACGAGAGCTTTCCAGAACCATTTGCTGATCCCGAGCGATTAGTTTGGCAAGTGTTGAATGCCGGGTGGGATGTTGGACTACCCGTCGTTAGTGAGAATGCACTTCCTTGCCTAAACAGAGTTAGCTATAACAAGGTTTTGGATAATACCAAGCCCATTAATGATCCAGATGGAAGGCACTTTTCATCCTTTACATACCTGAGGCTCAGTCCACTTCTGATGGAACGACCAAACTTCATAGAGTTTGAACGATTTGTCAAGAGGATGCACGGAGAAGCTGTTCTTGATCTTCAGGTATAA
Protein sequence

>MsaT038527.1

MVEIDGGSGMGTGAGDNRFQQHHEFQEQAGTPGGGNRRSRPVEEKERTKLRERRRRAITARILAGLRRHGNYNLRVRADINDVIAALAREAGWVVLPDGTTFPSKSQGQMPAGGNSAVVTSSSSHVPSQQPPSVSLKGVASGYGSPLDYNACPMKDVFIPSPSPYDLSSTSRSQTSMVGDGEAQRDNPPLIDGSIDTINEKQIADIPPRLPERDFAGSPYVPVYVMLPLGVINIKCELVDPDGILKQLRVLKSANVDGVMVDCWWGIVEAHAPQEYNWNGYKRLFQMVRELKLKLQVVMSFHECGGNFGDDVCIPLPHWIAEIGRSNPDIFFTDREGRHNPECLSWGIDKERVLRGRTAVEVYFDFMRSFRIEFNEYFEDGFISMIEVGLGPCGELRYPSCPVKHGWRYPGIGEFQCYDQYMLKSLRKAAEERGHTIWARGPDNVGTYNSQPHETGFFCDGGDYDGFYGRFFLNWYSQALVDHGNRVLSMAKLAFEGSCIAAKISGIYWWYKTASHAAELAAGYYNPCNRDGYAAITAMLKRNGVNLNIACVDLHTFNQHESFPEPFADPERLVWQVLNAGWDVGLPVVSENALPCLNRVSYNKVLDNTKPINDPDGRHFSSFTYLRLSPLLMERPNFIEFERFVKRMHGEAVLDLQV*