AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038630 MtrunA17_Chr7g0248691 96.178 314 12 0 1 314 1 314 0.0 621
MsaG038630 MtrunA17_Chr8g0348311 53.392 339 125 6 1 312 1 333 1.90e-89 271
MsaG038630 MtrunA17_Chr2g0293481 49.000 200 72 6 135 306 238 435 2.61e-48 169
MsaG038630 MtrunA17_Chr1g0202461 66.667 111 35 2 132 240 238 348 1.85e-44 156
MsaG038630 MtrunA17_Chr8g0387501 41.048 229 93 7 4 226 9 201 3.78e-40 142
MsaG038630 MtrunA17_Chr2g0284321 60.440 91 30 1 134 218 9 99 1.63e-33 123
MsaG038630 MtrunA17_Chr7g0270051 49.635 137 53 3 134 261 24 153 2.77e-33 124
MsaG038630 MtrunA17_Chr8g0379461 49.587 121 40 2 135 243 45 156 4.79e-32 120
MsaG038630 MtrunA17_Chr2g0319961 58.947 95 28 1 135 218 50 144 2.32e-31 118
MsaG038630 MtrunA17_Chr1g0147881 62.195 82 26 2 135 211 238 319 7.09e-29 114
MsaG038630 MtrunA17_Chr4g0071521 48.780 123 42 3 102 211 188 302 4.27e-28 111
MsaG038630 MtrunA17_Chr5g0401821 60.241 83 28 1 134 211 174 256 2.29e-27 108
MsaG038630 MtrunA17_Chr4g0053281 60.976 82 27 2 135 211 242 323 1.76e-26 107
MsaG038630 MtrunA17_Chr2g0289181 51.136 88 42 1 141 227 1 88 7.79e-25 100
MsaG038630 MtrunA17_Chr1g0184691 57.500 80 29 1 137 211 271 350 3.58e-24 101
MsaG038630 MtrunA17_Chr3g0139021 43.478 115 53 4 117 219 266 380 2.63e-23 99.4
MsaG038630 MtrunA17_Chr7g0228941 49.367 79 35 1 138 211 31 109 2.90e-22 90.5
MsaG038630 MtrunA17_Chr4g0051881 59.016 61 24 1 155 215 179 238 8.48e-19 85.9
MsaG038630 MtrunA17_Chr7g0270831 52.055 73 35 0 152 224 214 286 2.66e-18 85.1
MsaG038630 MtrunA17_Chr7g0270191 48.148 81 39 2 155 235 146 223 3.38e-18 83.6
MsaG038630 MtrunA17_Chr7g0218081 43.564 101 55 2 132 231 387 486 3.49e-18 85.1
MsaG038630 MtrunA17_Chr7g0218081 30.496 141 80 5 85 214 153 286 3.41e-11 63.9
MsaG038630 MtrunA17_Chr1g0194481 41.176 102 49 4 130 222 127 226 5.15e-18 83.6
MsaG038630 MtrunA17_Chr5g0418191 46.237 93 46 3 128 217 291 382 8.48e-18 83.6
MsaG038630 MtrunA17_Chr5g0418191 32.653 147 71 5 87 214 72 209 5.92e-11 63.2
MsaG038630 MtrunA17_Chr7g0263531 45.918 98 36 3 133 214 324 420 2.15e-17 82.4
MsaG038630 MtrunA17_Chr7g0263531 45.833 72 37 2 153 224 188 257 8.60e-13 68.9
MsaG038630 MtrunA17_Chr2g0288871 54.688 64 29 0 151 214 302 365 2.31e-17 82.8
MsaG038630 MtrunA17_Chr5g0443131 54.098 61 27 1 155 215 158 217 2.43e-17 81.3
MsaG038630 MtrunA17_Chr3g0126391 34.177 158 95 4 155 310 134 284 4.78e-17 80.9
MsaG038630 MtrunA17_Chr4g0042831 46.154 91 46 3 129 217 251 340 9.36e-17 80.5
MsaG038630 MtrunA17_Chr4g0042831 41.333 75 43 1 140 214 131 204 6.66e-12 65.9
MsaG038630 MtrunA17_Chr3g0101131 42.157 102 45 3 131 219 379 479 1.53e-16 80.5
MsaG038630 MtrunA17_Chr4g0025051 48.000 75 39 0 150 224 331 405 1.71e-16 80.1
MsaG038630 MtrunA17_Chr4g0025061 45.333 75 41 0 150 224 356 430 5.97e-16 78.6
MsaG038630 MtrunA17_Chr3g0099641 44.595 74 41 0 151 224 248 321 6.41e-16 78.2
MsaG038630 MtrunA17_Chr7g0216891 40.659 91 51 1 134 224 202 289 6.98e-16 78.6
MsaG038630 MtrunA17_Chr4g0051181 53.226 62 29 0 154 215 243 304 7.01e-16 78.2
MsaG038630 MtrunA17_Chr5g0445311 38.739 111 48 4 131 222 325 434 8.94e-16 78.2
MsaG038630 MtrunA17_Chr4g0001361 43.243 74 42 0 152 225 160 233 2.02e-15 75.9
MsaG038630 MtrunA17_Chr5g0418781 56.140 57 24 1 155 211 125 180 2.06e-15 75.1
MsaG038630 MtrunA17_Chr7g0238921 36.000 150 82 5 152 300 122 258 2.14e-15 75.9
MsaG038630 MtrunA17_Chr7g0275531 43.299 97 42 3 131 214 88 184 2.64e-15 75.5
MsaG038630 MtrunA17_Chr4g0075201 50.794 63 31 0 152 214 265 327 2.67e-15 76.6
MsaG038630 MtrunA17_Chr2g0302391 50.000 70 35 0 155 224 212 281 3.94e-15 75.9
MsaG038630 MtrunA17_Chr8g0387981 42.353 85 41 2 133 209 105 189 4.00e-15 75.5
MsaG038630 MtrunA17_Chr2g0322391 42.683 82 35 1 133 214 340 409 5.07e-15 75.9
MsaG038630 MtrunA17_Chr2g0333851 43.077 65 37 0 152 216 167 231 8.20e-15 73.9
MsaG038630 MtrunA17_Chr7g0249311 36.207 116 66 4 105 219 348 456 8.97e-15 75.1
MsaG038630 MtrunA17_Chr7g0249311 42.466 73 40 2 142 214 167 237 3.76e-11 64.3
MsaG038630 MtrunA17_Chr2g0294231 47.541 61 31 1 155 215 204 263 1.08e-14 74.3
MsaG038630 MtrunA17_Chr3g0079691 49.231 65 32 1 155 219 496 559 3.18e-14 73.6
MsaG038630 MtrunA17_Chr3g0079691 50.000 62 29 2 153 214 279 338 7.55e-12 66.2
MsaG038630 MtrunA17_Chr4g0060271 41.975 81 44 1 134 214 238 315 5.13e-14 72.8
MsaG038630 MtrunA17_Chr8g0347251 28.358 201 122 5 114 303 96 285 5.93e-14 71.6
MsaG038630 MtrunA17_Chr6g0485431 51.389 72 31 3 150 218 150 220 6.04e-14 72.0
MsaG038630 MtrunA17_Chr3g0129321 44.615 65 35 1 152 216 116 179 6.24e-14 69.7
MsaG038630 MtrunA17_Chr4g0074001 48.485 66 33 1 152 217 357 421 6.82e-14 72.4
MsaG038630 MtrunA17_Chr5g0447701 43.373 83 44 2 152 234 250 329 7.70e-14 71.6
MsaG038630 MtrunA17_Chr4g0004911 51.667 60 28 1 155 214 337 395 9.65e-14 71.6
MsaG038630 MtrunA17_Chr4g0004911 45.000 60 32 1 155 214 185 243 7.92e-11 62.8
MsaG038630 MtrunA17_Chr8g0383251 47.619 63 32 1 152 214 106 167 9.74e-14 68.6
MsaG038630 MtrunA17_Chr1g0150281 47.368 57 30 0 155 211 141 197 1.00e-13 70.5
MsaG038630 MtrunA17_Chr1g0150801 51.724 58 27 1 155 212 103 159 1.25e-13 67.8
MsaG038630 MtrunA17_Chr7g0243891 34.437 151 70 6 109 231 310 459 1.52e-13 71.2
MsaG038630 MtrunA17_Chr7g0249301 49.180 61 30 1 155 215 293 352 1.63e-13 70.9
MsaG038630 MtrunA17_Chr7g0249301 49.180 61 29 2 155 215 63 121 4.79e-12 66.2
MsaG038630 MtrunA17_Chr4g0068541 44.262 61 33 1 155 215 209 268 2.08e-13 70.5
MsaG038630 MtrunA17_Chr3g0136961 40.789 76 38 1 143 211 120 195 6.37e-13 68.2
MsaG038630 MtrunA17_Chr4g0024391 42.667 75 43 0 150 224 225 299 7.26e-13 68.9
MsaG038630 MtrunA17_Chr3g0106491 45.333 75 39 2 155 229 366 438 9.93e-13 68.9
MsaG038630 MtrunA17_Chr3g0106491 30.588 170 87 6 64 215 101 257 5.83e-11 63.2
MsaG038630 MtrunA17_Chr7g0224801 44.262 61 33 1 152 212 91 150 1.86e-12 64.7
MsaG038630 MtrunA17_Chr5g0432161 47.692 65 33 1 155 219 422 485 2.08e-12 67.8
MsaG038630 MtrunA17_Chr1g0189391 50.794 63 29 2 150 211 93 154 2.25e-12 67.0
MsaG038630 MtrunA17_Chr8g0383351 44.118 68 31 2 150 211 150 216 5.78e-12 64.7
MsaG038630 MtrunA17_Chr3g0129361 46.053 76 37 3 155 230 145 216 5.96e-12 64.3
MsaG038630 MtrunA17_Chr8g0335811 41.573 89 47 3 126 211 124 210 8.03e-12 63.9
MsaG038630 MtrunA17_Chr1g0150271 40.625 64 38 0 152 215 99 162 9.13e-12 64.3
MsaG038630 MtrunA17_Chr1g0188941 45.902 61 32 1 155 215 52 111 4.47e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038630 AT4G01250.1 47.840 324 101 12 1 306 1 274 6.96e-77 238
MsaG038630 AT5G52830.1 39.274 303 128 6 3 267 4 288 1.75e-43 153
MsaG038630 AT4G23550.1 42.126 254 115 6 3 249 8 236 4.83e-41 145
MsaG038630 AT2G34830.1 68.367 98 21 1 135 222 186 283 5.37e-41 148
MsaG038630 AT2G34830.2 68.367 98 21 1 135 222 195 292 7.13e-41 148
MsaG038630 AT1G30650.1 62.617 107 27 2 129 222 179 285 1.69e-39 144
MsaG038630 AT5G45050.2 58.879 107 43 1 117 222 1114 1220 5.13e-36 138
MsaG038630 AT5G45050.1 58.879 107 43 1 117 222 1142 1248 5.51e-36 138
MsaG038630 AT5G45050.4 58.333 108 44 1 116 222 1002 1109 6.94e-36 138
MsaG038630 AT3G58710.2 51.695 118 38 2 129 227 34 151 6.54e-35 128
MsaG038630 AT1G29280.1 57.944 107 28 3 131 226 41 141 1.41e-33 124
MsaG038630 AT3G58710.1 53.571 112 33 2 135 227 41 152 1.70e-33 125
MsaG038630 AT2G30590.1 58.333 84 30 1 134 212 288 371 3.25e-27 110
MsaG038630 AT4G24240.1 59.036 83 29 1 134 211 256 338 1.99e-26 107
MsaG038630 AT4G31550.2 44.628 121 56 2 102 211 182 302 2.68e-26 106
MsaG038630 AT4G31550.1 56.098 82 31 1 135 211 222 303 1.18e-25 105
MsaG038630 AT2G23320.1 55.422 83 32 2 134 211 215 297 5.97e-25 103
MsaG038630 AT2G24570.1 56.098 82 31 1 135 211 219 300 7.94e-25 102
MsaG038630 AT5G45260.1 50.526 95 44 2 133 225 1188 1281 1.92e-24 104
MsaG038630 AT3G04670.1 55.422 83 32 1 134 211 237 319 2.09e-23 99.0
MsaG038630 AT5G28650.1 55.422 83 32 1 134 211 237 319 2.24e-23 99.0
MsaG038630 AT5G46350.1 52.778 72 31 2 155 224 184 254 4.27e-19 87.0
MsaG038630 AT4G18170.1 48.780 82 40 2 135 216 154 233 1.23e-18 85.5
MsaG038630 AT2G37260.3 53.846 91 35 4 127 211 236 325 1.49e-18 85.5
MsaG038630 AT2G37260.2 53.846 91 35 4 127 211 234 323 1.49e-18 85.5
MsaG038630 AT2G37260.1 53.846 91 35 4 127 211 316 405 2.38e-18 85.9
MsaG038630 AT4G04450.2 50.685 73 36 0 152 224 196 268 8.70e-18 84.0
MsaG038630 AT1G29860.1 55.738 61 26 1 155 215 137 196 1.10e-17 82.0
MsaG038630 AT4G04450.1 50.685 73 36 0 152 224 290 362 1.18e-17 84.0
MsaG038630 AT2G04880.1 37.795 127 67 4 120 240 272 392 2.07e-17 83.2
MsaG038630 AT2G04880.2 44.681 94 41 3 152 240 281 368 2.54e-17 82.8
MsaG038630 AT1G68150.1 46.053 76 41 0 154 229 235 310 6.90e-17 80.9
MsaG038630 AT4G22070.4 53.125 64 30 0 151 214 294 357 9.26e-17 81.3
MsaG038630 AT4G22070.3 53.125 64 30 0 151 214 294 357 9.26e-17 81.3
MsaG038630 AT4G22070.2 53.125 64 30 0 151 214 294 357 9.26e-17 81.3
MsaG038630 AT4G22070.1 53.125 64 30 0 151 214 294 357 9.26e-17 81.3
MsaG038630 AT1G62300.1 53.968 63 29 0 152 214 310 372 1.06e-16 81.3
MsaG038630 AT1G13960.2 53.968 63 28 1 152 214 254 315 1.32e-16 80.1
MsaG038630 AT1G13960.2 43.939 66 35 2 149 214 71 134 5.76e-11 63.5
MsaG038630 AT1G13960.1 53.968 63 28 1 152 214 407 468 1.93e-16 80.5
MsaG038630 AT1G13960.1 43.939 66 35 2 149 214 224 287 6.47e-11 63.5
MsaG038630 AT5G49520.1 53.333 60 27 1 155 214 222 280 3.29e-16 79.3
MsaG038630 AT2G03340.1 50.000 68 33 1 152 219 413 479 4.27e-16 79.3
MsaG038630 AT3G62340.1 46.988 83 38 2 137 214 96 177 5.73e-16 77.4
MsaG038630 AT5G26170.1 53.968 63 27 2 149 211 109 169 9.18e-16 74.3
MsaG038630 AT4G01720.1 54.098 61 28 0 154 214 239 299 1.66e-15 77.4
MsaG038630 AT5G15130.1 41.111 90 50 1 135 224 211 297 1.86e-15 77.4
MsaG038630 AT5G15130.2 41.111 90 50 1 135 224 169 255 2.28e-15 77.0
MsaG038630 AT2G21900.1 49.180 61 30 1 155 215 110 169 2.34e-15 73.9
MsaG038630 AT2G25000.2 48.333 60 31 0 155 214 104 163 2.46e-15 74.7
MsaG038630 AT2G25000.4 48.333 60 31 0 155 214 104 163 2.46e-15 74.7
MsaG038630 AT1G18860.1 43.750 80 42 1 135 214 223 299 3.50e-15 76.6
MsaG038630 AT2G25000.3 48.333 60 31 0 155 214 126 185 4.36e-15 74.3
MsaG038630 AT4G26640.5 46.835 79 36 2 146 219 192 269 5.08e-15 75.5
MsaG038630 AT4G26640.6 46.835 79 36 2 146 219 192 269 5.08e-15 75.5
MsaG038630 AT4G26640.4 46.835 79 36 2 146 219 192 269 5.08e-15 75.5
MsaG038630 AT4G26640.3 46.835 79 36 2 146 219 192 269 5.08e-15 75.5
MsaG038630 AT2G25000.1 48.333 60 31 0 155 214 147 206 5.54e-15 74.3
MsaG038630 AT4G26640.1 46.835 79 36 2 146 219 296 373 6.37e-15 75.9
MsaG038630 AT4G26640.2 46.835 79 36 2 146 219 368 445 6.42e-15 75.9
MsaG038630 AT4G26640.7 46.835 79 36 2 146 219 328 405 6.73e-15 75.9
MsaG038630 AT1G80840.1 40.278 72 43 0 155 226 147 218 1.10e-14 73.9
MsaG038630 AT4G11070.2 44.792 96 41 3 131 214 80 175 1.27e-14 73.6
MsaG038630 AT1G69310.4 52.632 57 26 1 155 211 148 203 1.31e-14 73.6
MsaG038630 AT1G69310.2 52.632 57 26 1 155 211 148 203 1.31e-14 73.6
MsaG038630 AT1G69310.1 52.632 57 26 1 155 211 148 203 1.31e-14 73.6
MsaG038630 AT4G11070.1 44.792 96 41 3 131 214 112 207 1.48e-14 73.6
MsaG038630 AT5G07100.2 50.000 64 31 1 152 215 139 201 1.55e-14 72.0
MsaG038630 AT5G07100.2 45.161 62 33 1 153 214 23 83 4.81e-11 62.0
MsaG038630 AT5G07100.4 50.000 64 31 1 152 215 139 201 1.55e-14 72.0
MsaG038630 AT5G07100.4 45.161 62 33 1 153 214 23 83 4.81e-11 62.0
MsaG038630 AT5G07100.5 50.000 64 31 1 152 215 139 201 1.55e-14 72.0
MsaG038630 AT5G07100.5 45.161 62 33 1 153 214 23 83 4.81e-11 62.0
MsaG038630 AT5G07100.3 50.000 64 31 1 152 215 139 201 1.55e-14 72.0
MsaG038630 AT5G07100.3 45.161 62 33 1 153 214 23 83 4.81e-11 62.0
MsaG038630 AT2G47260.1 39.286 84 49 2 131 214 152 233 1.57e-14 73.9
MsaG038630 AT3G01080.2 50.794 63 30 1 152 214 342 403 3.51e-14 73.6
MsaG038630 AT3G01080.2 41.429 70 39 2 153 222 204 271 9.51e-11 63.2
MsaG038630 AT2G46400.1 40.171 117 61 4 101 209 46 161 4.03e-14 72.0
MsaG038630 AT3G01080.1 50.794 63 30 1 152 214 304 365 4.07e-14 73.2
MsaG038630 AT5G07100.1 50.000 64 31 1 152 215 232 294 4.17e-14 72.4
MsaG038630 AT5G07100.1 45.161 62 33 1 153 214 116 176 8.72e-11 62.4
MsaG038630 AT1G69810.1 45.833 72 38 1 154 224 203 274 4.58e-14 72.8
MsaG038630 AT4G30935.1 46.970 66 34 1 152 217 329 393 5.21e-14 72.8
MsaG038630 AT1G69810.2 45.833 72 38 1 154 224 145 216 6.45e-14 72.0
MsaG038630 AT2G38470.1 46.479 71 37 1 155 225 363 432 7.86e-14 72.4
MsaG038630 AT4G31800.2 45.000 60 33 0 155 214 112 171 8.97e-14 70.5
MsaG038630 AT4G31800.1 45.000 60 33 0 155 214 177 236 1.09e-13 71.2
MsaG038630 AT5G56270.1 49.206 63 31 1 155 217 488 549 2.16e-13 71.2
MsaG038630 AT2G40740.1 41.837 98 45 4 130 215 142 239 2.57e-13 69.7
MsaG038630 AT1G64000.1 40.260 77 45 1 150 226 110 185 3.36e-13 67.8
MsaG038630 AT2G40740.3 42.708 96 43 4 132 215 153 248 3.57e-13 69.3
MsaG038630 AT4G26440.1 46.154 65 34 1 155 219 373 436 6.62e-13 69.7
MsaG038630 AT4G26440.1 41.463 82 40 4 144 223 172 247 3.30e-11 64.7
MsaG038630 AT4G23810.1 49.206 63 30 1 149 209 153 215 8.29e-13 68.6
MsaG038630 AT4G39410.1 39.394 99 55 3 136 230 203 300 9.29e-13 68.2
MsaG038630 AT5G41570.1 40.260 77 45 1 150 226 94 169 1.12e-12 65.9
MsaG038630 AT2G44745.1 42.045 88 46 3 127 211 116 201 1.40e-12 66.6
MsaG038630 AT2G30250.1 44.000 75 36 2 146 215 315 388 1.58e-12 68.2
MsaG038630 AT1G55600.1 44.262 61 33 1 154 214 307 366 1.74e-12 68.6
MsaG038630 AT5G13080.1 44.262 61 33 1 152 212 65 124 1.80e-12 64.7
MsaG038630 AT3G01970.1 35.802 81 51 1 152 232 63 142 1.86e-12 64.7
MsaG038630 AT3G56400.1 34.021 97 57 3 127 217 94 189 4.08e-12 66.2
MsaG038630 AT2G40750.1 32.609 138 74 4 149 267 146 283 5.03e-12 66.6
MsaG038630 AT2G46130.1 45.161 62 33 1 152 213 28 88 1.39e-11 61.2
MsaG038630 AT5G43290.1 45.455 77 39 2 152 228 112 185 1.65e-11 64.3
MsaG038630 AT5G64810.1 45.763 59 31 1 155 213 111 168 2.57e-11 62.4
MsaG038630 AT5G24110.1 44.444 63 33 1 149 209 108 170 6.41e-11 62.8
MsaG038630 AT3G01080.3 41.429 70 39 2 153 222 204 271 7.53e-11 62.8

Find 64 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTGTGTTTGAGCTAATT+TGG 0.118717 7:-78884780 None:intergenic
CTGCTATTGCAATTCCTAAT+TGG 0.183200 7:+78885610 MsaT038630.1:CDS
TTTGAAAGTTTAGATGAATT+TGG 0.224134 7:+78885567 MsaT038630.1:CDS
TCAGCTTGAGGTGGTGTTAA+TGG 0.224988 7:-78885372 None:intergenic
GATGAAGAAGGAGATGAATT+TGG 0.239015 7:+78885513 MsaT038630.1:CDS
TGTGGCTACTCTTCTATCTT+TGG 0.243238 7:+78884581 MsaT038630.1:CDS
TGCTATTGCAATTCCTAATT+GGG 0.291536 7:+78885611 MsaT038630.1:CDS
GCACTATTAGCAACCCAATT+AGG 0.295679 7:-78885624 None:intergenic
CACAAGAAGGTTCAGGTTGC+AGG 0.297705 7:-78884562 None:intergenic
AGAGTCTCTCTTCAGATATA+TGG 0.305175 7:+78885050 MsaT038630.1:CDS
GTAGAGAAAACGGAACATTT+TGG 0.319898 7:+78885760 MsaT038630.1:intron
TCTTGTGTTTGAGCTAATTT+GGG 0.330263 7:-78884779 None:intergenic
TCTTCACCTCCTGAGGTTGT+AGG 0.351765 7:-78885426 None:intergenic
GTCAGAAAGTAGAGAAGAAA+AGG 0.360877 7:+78885473 MsaT038630.1:CDS
CAAGAAAGTGACAAAATTTG+AGG 0.365124 7:-78884617 None:intergenic
ATTGCAATAGCAGAAGAAAA+TGG 0.365837 7:-78885600 None:intergenic
AGAAAAGGAAGATTCTTTGA+TGG 0.372979 7:+78885488 MsaT038630.1:CDS
GATCTCCATGCGGTGGTTAG+AGG 0.374060 7:+78884479 MsaT038630.1:CDS
ATATGGGCATGGAGAAAATA+TGG 0.375958 7:+78885067 MsaT038630.1:CDS
CTTTACCTATGGAAGAAGAT+TGG 0.382515 7:+78884456 None:intergenic
ATGGAGTTTCTAGCTTCAAA+AGG 0.418901 7:-78884650 None:intergenic
TATAGATGTAGCAGTTCAAA+AGG 0.420572 7:+78885219 MsaT038630.1:CDS
GAGTAGCCACAAGAAGGTTC+AGG 0.422256 7:-78884569 None:intergenic
TTTACCTATGGAAGAAGATT+GGG 0.432908 7:+78884457 None:intergenic
TCTTTGTTGGTTTCAGCTTG+AGG 0.464539 7:-78885384 None:intergenic
TGTGATGATCTTGAAAAGAA+AGG 0.468032 7:-78884698 None:intergenic
TATGTTACTATGAACATTGT+TGG 0.470318 7:-78885282 None:intergenic
GAGTCTCTCTTCAGATATAT+GGG 0.478312 7:+78885051 MsaT038630.1:CDS
AAGTTCATGCAACTCTTCAA+TGG 0.483711 7:-78884669 None:intergenic
AACCTCTTCTTCACCTCCTG+AGG 0.488504 7:-78885433 None:intergenic
CCCTCTTCTCCTACAACCTC+AGG 0.495834 7:+78885417 MsaT038630.1:CDS
AGCTAATTTGGGTGAAGCAG+AGG 0.511551 7:-78884768 None:intergenic
GAAGAAGAAACAGAGGATGT+AGG 0.525651 7:-78884527 None:intergenic
TGGAGTTTCTAGCTTCAAAA+GGG 0.530845 7:-78884649 None:intergenic
ACAAGAAGGTTCAGGTTGCA+GGG 0.532171 7:-78884561 None:intergenic
GGAGGAATCTTTGCTGACAA+TGG 0.535261 7:+78885781 MsaT038630.1:CDS
TCTGAAGAGAGACTCTCAAC+TGG 0.540075 7:-78885043 None:intergenic
CCTGAGGTTGTAGGAGAAGA+GGG 0.546404 7:-78885417 None:intergenic
AGGTGTGGTGGTTGAAGTAG+TGG 0.549041 7:-78884507 None:intergenic
GGAGAAGTTTGCAAAGAAAG+AGG 0.551853 7:-78884722 None:intergenic
TCTTTGATGGATGATGAAGA+AGG 0.562592 7:+78885501 MsaT038630.1:CDS
TCCTGAGGTTGTAGGAGAAG+AGG 0.567164 7:-78885418 None:intergenic
CTGCAACCTGAACCTTCTTG+TGG 0.572424 7:+78884563 MsaT038630.1:CDS
AGAAACAGAGGATGTAGGTG+TGG 0.573812 7:-78884522 None:intergenic
AGATCCCAATCTTCTTCCAT+AGG 0.576312 7:-78884461 None:intergenic
AACAGAGGAAGAAGAAACAG+AGG 0.577059 7:-78884534 None:intergenic
TAAGAGAATGAAGAAAACAA+AGG 0.578001 7:-78884743 None:intergenic
GAAGAAGAGGTTCAAACTCA+AGG 0.585892 7:+78885444 MsaT038630.1:CDS
ATAGAAGAGTAGCCACAAGA+AGG 0.596283 7:-78884575 None:intergenic
TAAAAGGGTCACCATATCCA+AGG 0.597269 7:+78885101 MsaT038630.1:CDS
TCTCTTCAGATATATGGGCA+TGG 0.605027 7:+78885056 MsaT038630.1:CDS
TTGTTGGTTTCAGCTTGAGG+TGG 0.614005 7:-78885381 None:intergenic
ACTCTCTTGCTGGTTCAACA+AGG 0.623552 7:+78885343 MsaT038630.1:CDS
ATAGATGTAGCAGTTCAAAA+GGG 0.629154 7:+78885220 MsaT038630.1:CDS
AACAGAGGATGTAGGTGTGG+TGG 0.629725 7:-78884519 None:intergenic
CAAGAAGGTTCAGGTTGCAG+GGG 0.629954 7:-78884560 None:intergenic
AACCTCAGGAGGTGAAGAAG+AGG 0.630119 7:+78885431 MsaT038630.1:CDS
AGCAACCTCTAACCACCGCA+TGG 0.645537 7:-78884484 None:intergenic
GGTTGGTGAGAGAACCACCT+TGG 0.648538 7:-78884835 None:intergenic
AGAAGTAAACATTCACACCA+AGG 0.691967 7:+78884818 MsaT038630.1:CDS
AGATTGGGATCTCCATGCGG+TGG 0.697339 7:+78884472 MsaT038630.1:CDS
TCTTCTCCTACAACCTCAGG+AGG 0.702219 7:+78885420 MsaT038630.1:CDS
AGTAAACATTCACACCAAGG+TGG 0.736776 7:+78884821 MsaT038630.1:CDS
AGAAGATTGGGATCTCCATG+CGG 0.744459 7:+78884469 MsaT038630.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 78884464 78885811 78884464 ID=MsaG038630
Chr7 mRNA 78884464 78885811 78884464 ID=MsaT038630.1;Parent=MsaG038630
Chr7 exon 78884464 78884873 78884464 ID=MsaT038630.1.exon1;Parent=MsaT038630.1
Chr7 CDS 78884464 78884873 78884464 ID=cds.MsaT038630.1;Parent=MsaT038630.1
Chr7 exon 78885012 78885122 78885012 ID=MsaT038630.1.exon2;Parent=MsaT038630.1
Chr7 CDS 78885012 78885122 78885012 ID=cds.MsaT038630.1;Parent=MsaT038630.1
Chr7 exon 78885212 78885632 78885212 ID=MsaT038630.1.exon3;Parent=MsaT038630.1
Chr7 CDS 78885212 78885632 78885212 ID=cds.MsaT038630.1;Parent=MsaT038630.1
Chr7 exon 78885764 78885811 78885764 ID=MsaT038630.1.exon4;Parent=MsaT038630.1
Chr7 CDS 78885764 78885811 78885764 ID=cds.MsaT038630.1;Parent=MsaT038630.1
Gene Sequence

>MsaT038630.1

ATGGAAGAAGATTGGGATCTCCATGCGGTGGTTAGAGGTTGCTCCACTACTTCAACCACCACACCTACATCCTCTGTTTCTTCTTCCTCTGTTTTTCCCCTGCAACCTGAACCTTCTTGTGGCTACTCTTCTATCTTTGGTAGTGAACAAAAACCTCAAATTTTGTCACTTTCTTGTTCTACACACCCTTTTGAAGCTAGAAACTCCATTGAAGAGTTGCATGAACTTTGCAAACCTTTCTTTTCAAGATCATCACAGCCTCTTTCTTTGCAAACTTCTCCTTTGTTTTCTTCATTCTCTTATTCCTCTGCTTCACCCAAATTAGCTCAAACACAAGAGAAACAACAACTTCAAAGAAGTAAACATTCACACCAAGGTGGTTCTCTCACCAACCCAAGATCAAAAAGAAGGAAAAATCAACTTAAGAAAGTTTGTCAAGTACCAGTTGAGAGTCTCTCTTCAGATATATGGGCATGGAGAAAATATGGTCAAAAACCTATAAAAGGGTCACCATATCCAAGGGGCTATTATAGATGTAGCAGTTCAAAAGGGTGTTTAGCAAGAAAACAAGTTGAAAGAAACAGAACAGATCCAACAATGTTCATAGTAACATATACATGTGAACACAATCATCCTGCTCCTACACACAAAAACTCTCTTGCTGGTTCAACAAGGCAGAAGCCATTAACACCACCTCAAGCTGAAACCAACAAAGATCCTACAAAACCCTCTTCTCCTACAACCTCAGGAGGTGAAGAAGAGGTTCAAACTCAAGGAGAAAAGTCAGAAAGTAGAGAAGAAAAGGAAGATTCTTTGATGGATGATGAAGAAGGAGATGAATTTGGTTTATCTGATGTTGTTTTAAGTGATGATTTCTTTGAAAGTTTAGATGAATTTGGTTTCACAGATCCATTTTCTTCTGCTATTGCAATTCCTAATTGGAGAAAACGGAACATTTTGGAGGAATCTTTGCTGACAATGGAAGTGTAG
Protein sequence

>MsaT038630.1

MEEDWDLHAVVRGCSTTSTTTPTSSVSSSSVFPLQPEPSCGYSSIFGSEQKPQILSLSCSTHPFEARNSIEELHELCKPFFSRSSQPLSLQTSPLFSSFSYSSASPKLAQTQEKQQLQRSKHSHQGGSLTNPRSKRRKNQLKKVCQVPVESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCLARKQVERNRTDPTMFIVTYTCEHNHPAPTHKNSLAGSTRQKPLTPPQAETNKDPTKPSSPTTSGGEEEVQTQGEKSESREEKEDSLMDDEEGDEFGLSDVVLSDDFFESLDEFGFTDPFSSAIAIPNWRKRNILEESLLTMEV*