AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038780 MtrunA17_Chr7g0250261 96.127 284 11 0 1 284 1 284 0.0 532
MsaG038780 MtrunA17_Chr0c29g0494001 96.127 284 11 0 1 284 1 284 0.0 532
MsaG038780 MtrunA17_Chr2g0304101 42.991 214 118 4 67 279 94 304 2.08e-46 158
MsaG038780 MtrunA17_Chr2g0311181 32.852 277 169 8 6 279 37 299 1.68e-35 130
MsaG038780 MtrunA17_Chr5g0417641 33.829 269 151 8 19 279 43 292 3.76e-32 120
MsaG038780 MtrunA17_Chr6g0448821 26.636 214 110 4 75 283 189 360 3.37e-19 86.7
MsaG038780 MtrunA17_Chr3g0094341 29.293 198 128 7 25 219 13 201 3.88e-17 78.6
MsaG038780 MtrunA17_Chr2g0294671 31.553 206 127 7 78 279 287 482 2.20e-16 79.0
MsaG038780 MtrunA17_Chr4g0043941 29.032 217 134 5 77 283 116 322 1.59e-15 75.5
MsaG038780 MtrunA17_Chr5g0445491 26.484 219 151 6 72 283 260 475 4.01e-15 75.1
MsaG038780 MtrunA17_Chr4g0034981 27.184 206 143 5 77 279 384 585 1.13e-14 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038780 AT3G18870.1 55.422 249 107 3 36 284 29 273 6.86e-96 283
MsaG038780 AT2G03050.2 36.667 240 146 5 43 279 33 269 8.67e-44 150
MsaG038780 AT2G03050.1 36.667 240 146 5 43 279 32 268 8.72e-44 150
MsaG038780 AT2G34620.1 39.524 210 123 4 71 279 84 290 2.88e-43 150
MsaG038780 AT2G36000.1 34.440 241 146 8 44 279 74 307 3.50e-31 119
MsaG038780 AT2G36000.2 34.440 241 146 8 44 279 74 307 3.92e-31 118
MsaG038780 AT1G78930.1 27.358 212 117 3 72 283 404 578 2.45e-17 82.4
MsaG038780 AT5G55580.1 27.854 219 148 7 72 283 253 468 6.91e-16 77.8
MsaG038780 AT5G55580.2 27.854 219 148 7 72 283 147 362 9.52e-16 77.0
MsaG038780 AT4G14605.2 27.404 208 110 5 72 279 301 467 5.72e-15 75.1
MsaG038780 AT4G14605.1 27.404 208 110 5 72 279 301 467 5.72e-15 75.1
MsaG038780 AT4G38160.4 28.037 214 141 6 77 283 117 324 9.33e-15 73.6
MsaG038780 AT4G38160.1 28.037 214 141 6 77 283 117 324 9.33e-15 73.6
MsaG038780 AT4G38160.2 28.037 214 141 6 77 283 117 324 1.08e-14 73.9
MsaG038780 AT4G38160.3 28.037 214 141 6 77 283 117 324 1.48e-14 73.6
MsaG038780 AT2G21710.1 26.860 242 132 5 78 279 356 592 1.73e-13 70.9

Find 107 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAGGTTGCCGGCGATAT+TGG 0.211818 7:-80440023 MsaT038780.1:CDS
GCAAGGTTGCCGGCGATATT+GGG 0.271051 7:-80440022 MsaT038780.1:CDS
TCCTGTTAGTTGGCCTGTTC+AGG 0.273978 7:+80440146 None:intergenic
GTGGCCGAAGTTTGTGTATT+TGG 0.292279 7:-80439978 MsaT038780.1:CDS
GGAGGGTTGGTTTGAGGATG+TGG 0.308413 7:+80440200 None:intergenic
GGAGAGGGAGGTAGGGGAAA+TGG 0.315974 7:+80440321 None:intergenic
TCAAGGTGAGGTAGGTTGTT+TGG 0.324999 7:+80440451 None:intergenic
TTTGGCGTTGGTGGAAAGAT+TGG 0.342499 7:+80440623 None:intergenic
TTGATGTAACCGTTTCTGTT+AGG 0.373078 7:+80440544 None:intergenic
ACCTGAACAGGCCAACTAAC+AGG 0.386362 7:-80440147 MsaT038780.1:CDS
GAGGATAAGAAGATGGAGTT+TGG 0.390964 7:+80440605 None:intergenic
GAATGTTTGTGCAAGGTTGC+CGG 0.395975 7:-80440032 MsaT038780.1:CDS
GGGACAATGGTGGACTAGCC+GGG 0.397061 7:+80440360 None:intergenic
ACGTCGGTGAAGAGGAGGTT+AGG 0.409537 7:+80440226 None:intergenic
ATTTGAAAGAAAGAGGTGTT+AGG 0.409873 7:-80439862 MsaT038780.1:CDS
GGAGGTAGGGGAAATGGAGG+TGG 0.413210 7:+80440327 None:intergenic
GAAAGAGATGGAGAGGGAGG+TGG 0.414641 7:-80439954 MsaT038780.1:CDS
GAGGGAGGTAGGGGAAATGG+AGG 0.418156 7:+80440324 None:intergenic
GGAAGTGGAGGGTTGGTTTG+AGG 0.419169 7:+80440194 None:intergenic
ATGGAGGTGGTGAAGAGGTT+GGG 0.419906 7:+80440340 None:intergenic
AAGATGGAGTTTGGCGTTGG+TGG 0.422753 7:+80440614 None:intergenic
AGGTTTCTTGAGGAAGTGGT+TGG 0.427900 7:-80440076 MsaT038780.1:CDS
AATGGAGGTGGTGAAGAGGT+TGG 0.433095 7:+80440339 None:intergenic
TGGGACAATGGTGGACTAGC+CGG 0.434927 7:+80440359 None:intergenic
TCTTTCAAATGCAAATGCCT+TGG 0.434972 7:+80439875 None:intergenic
TGAGAGGGAGGATAAGAAGA+TGG 0.437100 7:+80440598 None:intergenic
GGTTAGGGCAACGGAGGATA+AGG 0.439054 7:+80440242 None:intergenic
CAACTTCCTCGCGGAGGAAG+TGG 0.444919 7:+80440179 None:intergenic
CACTTCCTCCGCGAGGAAGT+TGG 0.448220 7:-80440178 MsaT038780.1:CDS
TTGGAGTTAGTAACCTGAAC+AGG 0.454578 7:-80440159 MsaT038780.1:CDS
AACCGTTTCTGTTAGGATAA+CGG 0.454941 7:+80440551 None:intergenic
CATTTGCATTTGAAAGAAAG+AGG 0.462822 7:-80439869 MsaT038780.1:CDS
GAGGCGAGGAAGGTGAAGAC+GGG 0.462849 7:+80440298 None:intergenic
GGTTTCTTGAGGAAGTGGTT+GGG 0.466379 7:-80440075 MsaT038780.1:CDS
GATGGTGAGGATGTGGTTGA+TGG 0.467160 7:+80440426 None:intergenic
GTTGAGGTTTCTTGAGGAAG+TGG 0.478350 7:-80440080 MsaT038780.1:CDS
CGGTGAAAGAGTGGGATTTG+AGG 0.484295 7:+80440395 None:intergenic
TGGTGAAGAGGTTGGGACAA+TGG 0.486305 7:+80440347 None:intergenic
GGCGTTGGTGGAAAGATTGG+AGG 0.493018 7:+80440626 None:intergenic
TATAGAGTTTGATGATTGAG+AGG 0.494375 7:+80440582 None:intergenic
AGAAGATGCGCATGAGGTTG+AGG 0.495323 7:-80440096 MsaT038780.1:CDS
GAGTTTGATGATTGAGAGGG+AGG 0.502670 7:+80440586 None:intergenic
GAGGAGGTCGGAGGCGAGGA+AGG 0.505171 7:+80440288 None:intergenic
TTGGGTTTAGTTTGGAGAAG+AGG 0.509649 7:-80439904 MsaT038780.1:CDS
GGAGGCGAGGAAGGTGAAGA+CGG 0.509867 7:+80440297 None:intergenic
AACCGTTATCCTAACAGAAA+CGG 0.513485 7:-80440553 MsaT038780.1:CDS
AAGAAGATGGAGTTTGGCGT+TGG 0.515595 7:+80440611 None:intergenic
TGATGGTGTCAAGGTGAGGT+AGG 0.516204 7:+80440443 None:intergenic
TCACCGAGGCCGACATCCCC+CGG 0.518020 7:-80440378 MsaT038780.1:CDS
TGGCGAGGAGGTCGGAGGCG+AGG 0.519629 7:+80440284 None:intergenic
TTCACCAAATACACAAACTT+CGG 0.521850 7:+80439974 None:intergenic
AGATGCGCGTTCCTGTTAGT+TGG 0.527130 7:+80440136 None:intergenic
CGTCGGTGAAGAGGAGGTTA+GGG 0.540321 7:+80440227 None:intergenic
ATTTGGTGAAAGAGATGGAG+AGG 0.546039 7:-80439961 MsaT038780.1:CDS
CACATCAACATTCTATTCAA+AGG 0.546735 7:+80439836 None:intergenic
TTTCTGTTAGGATAACGGTT+AGG 0.548102 7:+80440556 None:intergenic
TCGCGGAGGAAGTGGAGGGT+TGG 0.548697 7:+80440187 None:intergenic
GGTGAAAGAGATGGAGAGGG+AGG 0.550774 7:-80439957 MsaT038780.1:CDS
ATAGAGTTTGATGATTGAGA+GGG 0.551408 7:+80440583 None:intergenic
CTTCCTCGCGGAGGAAGTGG+AGG 0.556212 7:+80440182 None:intergenic
TGAAGAGGAGGTTAGGGCAA+CGG 0.557020 7:+80440233 None:intergenic
CATGAGGTTGAGGTTTCTTG+AGG 0.557913 7:-80440086 MsaT038780.1:CDS
CAAGGTTGCCGGCGATATTG+GGG 0.558881 7:-80440021 MsaT038780.1:CDS
GGGAAATGGAGGTGGTGAAG+AGG 0.559827 7:+80440335 None:intergenic
TGTCGGCCTCGGTGAAAGAG+TGG 0.565492 7:+80440386 None:intergenic
ATGTGATGATGGTGAGGATG+TGG 0.567993 7:+80440419 None:intergenic
TGTGTATTTGGTGAAAGAGA+TGG 0.569565 7:-80439966 MsaT038780.1:CDS
TGGAGAAGAGGATTGTTCCA+AGG 0.570343 7:-80439892 MsaT038780.1:CDS
CTTTGAATAGAATGTTGATG+TGG 0.576656 7:-80439835 MsaT038780.1:CDS
TTTGAATAGAATGTTGATGT+GGG 0.578021 7:-80439834 MsaT038780.1:CDS
TTTGGTGAAAGAGATGGAGA+GGG 0.581785 7:-80439960 MsaT038780.1:CDS
GGACAATGGTGGACTAGCCG+GGG 0.587321 7:+80440361 None:intergenic
TGAGGAATGTGATGATGGTG+AGG 0.588656 7:+80440413 None:intergenic
TGTGGTTTACGTCGGTGAAG+AGG 0.589418 7:+80440218 None:intergenic
TTCCACAGTGGCGAGGAGGT+CGG 0.594168 7:+80440276 None:intergenic
CACAGTGGCGAGGAGGTCGG+AGG 0.596584 7:+80440279 None:intergenic
AAGCGAGGAATGTTTGTGCA+AGG 0.596914 7:-80440039 MsaT038780.1:CDS
GGATTTGAGGAATGTGATGA+TGG 0.599559 7:+80440408 None:intergenic
GGATGTGGTTGATGGTGTCA+AGG 0.602083 7:+80440434 None:intergenic
AGTGGTTGGGTTTACGTACG+AGG 0.606381 7:-80440062 MsaT038780.1:CDS
ATGATAAGTTCTATGCAAAG+TGG 0.608848 7:-80439808 MsaT038780.1:CDS
ACATCGTACCCCAATATCGC+CGG 0.610968 7:+80440013 None:intergenic
GGTTTACGTACGAGGAAGCG+AGG 0.621107 7:-80440054 MsaT038780.1:CDS
GTCGGCCTCGGTGAAAGAGT+GGG 0.623113 7:+80440387 None:intergenic
GGTTTACGTCGGTGAAGAGG+AGG 0.624023 7:+80440221 None:intergenic
AGGCGAGGAAGGTGAAGACG+GGG 0.626763 7:+80440299 None:intergenic
GGCGATATTGGGGTACGATG+TGG 0.628281 7:-80440011 MsaT038780.1:CDS
TGGTTGATGGTGTCAAGGTG+AGG 0.634445 7:+80440439 None:intergenic
GGCGAGGAAGGTGAAGACGG+GGG 0.649632 7:+80440300 None:intergenic
TTTGAGGATGTGGTTTACGT+CGG 0.649863 7:+80440210 None:intergenic
TTCCTCGCGGAGGAAGTGGA+GGG 0.650738 7:+80440183 None:intergenic
GAGAGTCTTCCACAGTGGCG+AGG 0.656874 7:+80440269 None:intergenic
CTCCGACCTCCTCGCCACTG+TGG 0.667154 7:-80440278 MsaT038780.1:CDS
GCATCTTCTCAACACGCGTG+TGG 0.668292 7:-80440119 MsaT038780.1:CDS
GACAATGGTGGACTAGCCGG+GGG 0.669422 7:+80440362 None:intergenic
AGTCTTCCACAGTGGCGAGG+AGG 0.672229 7:+80440272 None:intergenic
AGAGGAGGTTAGGGCAACGG+AGG 0.682019 7:+80440236 None:intergenic
GTGTGGAGAAGATGCGCATG+AGG 0.685391 7:-80440102 MsaT038780.1:CDS
AACCCTCCACTTCCTCCGCG+AGG 0.690297 7:-80440185 MsaT038780.1:CDS
TGAAGAGGTTGGGACAATGG+TGG 0.704696 7:+80440350 None:intergenic
ACGATGTGGAGAATAACTTG+TGG 0.706381 7:-80439997 MsaT038780.1:CDS
GCCACTGTGGAAGACTCTCG+CGG 0.706536 7:-80440265 MsaT038780.1:CDS
TACTAACTCCAACTTCCTCG+CGG 0.708475 7:+80440170 None:intergenic
TTGAATAGAATGTTGATGTG+GGG 0.710612 7:-80439833 MsaT038780.1:CDS
TAACTCCAACTTCCTCGCGG+AGG 0.712692 7:+80440173 None:intergenic
GCCGCGAGAGTCTTCCACAG+TGG 0.750300 7:+80440264 None:intergenic
CAAATCCCACTCTTTCACCG+AGG 0.751367 7:-80440392 MsaT038780.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 80439802 80440656 80439802 ID=MsaG038780
Chr7 mRNA 80439802 80440656 80439802 ID=MsaT038780.1;Parent=MsaG038780
Chr7 exon 80439802 80440656 80439802 ID=MsaT038780.1.exon1;Parent=MsaT038780.1
Chr7 CDS 80439802 80440656 80439802 ID=cds.MsaT038780.1;Parent=MsaT038780.1
Gene Sequence

>MsaT038780.1

ATGGTCTTCCTCCAATCTTTCCACCAACGCCAAACTCCATCTTCTTATCCTCCCTCTCAATCATCAAACTCTATAGTACCTAACCGTTATCCTAACAGAAACGGTTACATCAAGTTCAGAACAACCTACAATGAAAATCTCCATTACCTAAAATCACTAACCATAATAAACCCCAACACAAAACCAAACAACCTACCTCACCTTGACACCATCAACCACATCCTCACCATCATCACATTCCTCAAATCCCACTCTTTCACCGAGGCCGACATCCCCCGGCTAGTCCACCATTGTCCCAACCTCTTCACCACCTCCATTTCCCCTACCTCCCTCTCCCCCGTCTTCACCTTCCTCGCCTCCGACCTCCTCGCCACTGTGGAAGACTCTCGCGGCCTTATCCTCCGTTGCCCTAACCTCCTCTTCACCGACGTAAACCACATCCTCAAACCAACCCTCCACTTCCTCCGCGAGGAAGTTGGAGTTAGTAACCTGAACAGGCCAACTAACAGGAACGCGCATCTTCTCAACACGCGTGTGGAGAAGATGCGCATGAGGTTGAGGTTTCTTGAGGAAGTGGTTGGGTTTACGTACGAGGAAGCGAGGAATGTTTGTGCAAGGTTGCCGGCGATATTGGGGTACGATGTGGAGAATAACTTGTGGCCGAAGTTTGTGTATTTGGTGAAAGAGATGGAGAGGGAGGTGGAAGAGTTGAAGAAGTTTCCGCAGTTTTTTGGGTTTAGTTTGGAGAAGAGGATTGTTCCAAGGCATTTGCATTTGAAAGAAAGAGGTGTTAGGATTCCTTTGAATAGAATGTTGATGTGGGGTGATGATAAGTTCTATGCAAAGTGGAAGTGA
Protein sequence

>MsaT038780.1

MVFLQSFHQRQTPSSYPPSQSSNSIVPNRYPNRNGYIKFRTTYNENLHYLKSLTIINPNTKPNNLPHLDTINHILTIITFLKSHSFTEADIPRLVHHCPNLFTTSISPTSLSPVFTFLASDLLATVEDSRGLILRCPNLLFTDVNHILKPTLHFLREEVGVSNLNRPTNRNAHLLNTRVEKMRMRLRFLEEVVGFTYEEARNVCARLPAILGYDVENNLWPKFVYLVKEMEREVEELKKFPQFFGFSLEKRIVPRHLHLKERGVRIPLNRMLMWGDDKFYAKWK*