Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG038882 | XP_013449453.1 | 76.020 | 196 | 9 | 3 | 1 | 160 | 1 | 194 | 1.58e-96 | 287 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG038882 | tr|I3SX53|I3SX53_MEDTR | 76.020 | 196 | 9 | 3 | 1 | 160 | 1 | 194 | 7.56e-97 | 287 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG038882 | TF | NAC |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG038882 | MtrunA17_Chr7g0251651 | 76.020 | 196 | 9 | 3 | 1 | 160 | 1 | 194 | 1.46e-100 | 287 |
| MsaG038882 | MtrunA17_Chr7g0251671 | 72.897 | 107 | 28 | 1 | 15 | 121 | 43 | 148 | 2.83e-54 | 169 |
| MsaG038882 | MtrunA17_Chr7g0251661 | 70.093 | 107 | 31 | 1 | 15 | 121 | 43 | 148 | 6.39e-50 | 157 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGAACACATTTGTTTAC+TGG | 0.255170 | 7:-81936680 | MsaT038882.1:CDS |
| GGTACTATTTCTTTGATATC+AGG | 0.295817 | 7:-81936809 | MsaT038882.1:CDS |
| GAACCGTAGGTTCGAAAATA+TGG | 0.299363 | 7:-81936787 | MsaT038882.1:CDS |
| AAAGCATAGTGAGCTAGTTT+TGG | 0.338409 | 7:-81937207 | MsaT038882.1:CDS |
| TGAGTTCATGGAGAGGGAGG+AGG | 0.476558 | 7:+81936231 | None:intergenic |
| AAATCAATCACTGTTGGTGT+GGG | 0.492009 | 7:+81936282 | None:intergenic |
| GAGTTCATGGAGAGGGAGGA+GGG | 0.516664 | 7:+81936232 | None:intergenic |
| AATATGGATGCAAGAAGAGC+TGG | 0.518416 | 7:-81936771 | MsaT038882.1:CDS |
| GAGCCATGGAAGCTGCCAAA+AGG | 0.533677 | 7:-81937050 | MsaT038882.1:intron |
| GAAATCAATCACTGTTGGTG+TGG | 0.538024 | 7:+81936281 | None:intergenic |
| GAAGAGCTGGAAATGGTGAA+TGG | 0.539470 | 7:-81936758 | MsaT038882.1:CDS |
| AGTTCATGGAGAGGGAGGAG+GGG | 0.541559 | 7:+81936233 | None:intergenic |
| TTTGTTTACTGGAGAATGCA+AGG | 0.541628 | 7:-81936669 | MsaT038882.1:CDS |
| CTATCGGATATTTAAGATGA+AGG | 0.543622 | 7:-81936328 | MsaT038882.1:CDS |
| GGACCTCTTCAAAGCATATG+AGG | 0.554252 | 7:-81936830 | MsaT038882.1:CDS |
| ATGTATTTCAAACTGAGCCA+TGG | 0.561975 | 7:-81937064 | MsaT038882.1:CDS |
| TGAAACATGAGTTCATGGAG+AGG | 0.563520 | 7:+81936224 | None:intergenic |
| GATGCAAGAAGAGCTGGAAA+TGG | 0.566075 | 7:-81936765 | MsaT038882.1:CDS |
| ATCAATCACTGTTGGTGTGG+GGG | 0.572846 | 7:+81936284 | None:intergenic |
| ATGTAGGTGTACTCAGCTGT+AGG | 0.589112 | 7:-81936354 | MsaT038882.1:intron |
| ACTCAGCTGTAGGTGCCTAT+CGG | 0.590616 | 7:-81936344 | MsaT038882.1:CDS |
| GTACCTCATATGCTTTGAAG+AGG | 0.594155 | 7:+81936827 | None:intergenic |
| TCTTTGATATCAGGAACCGT+AGG | 0.597083 | 7:-81936800 | MsaT038882.1:CDS |
| AGAGACACTTGCATCTTCCA+TGG | 0.599268 | 7:+81936257 | None:intergenic |
| CTTCATCTTAAATATCCGAT+AGG | 0.611455 | 7:+81936329 | None:intergenic |
| ACATGAGTTCATGGAGAGGG+AGG | 0.614894 | 7:+81936228 | None:intergenic |
| GTGAATGGAGAATATATGAG+AGG | 0.620604 | 7:-81936743 | MsaT038882.1:CDS |
| GAAACATGAGTTCATGGAGA+GGG | 0.632009 | 7:+81936225 | None:intergenic |
| AATCAATCACTGTTGGTGTG+GGG | 0.641402 | 7:+81936283 | None:intergenic |
| AGGATGCATGAATTTGTCAT+CGG | 0.657194 | 7:-81936618 | MsaT038882.1:CDS |
| GTCAAGCTCTGATGACACAA+TGG | 0.657620 | 7:-81936641 | MsaT038882.1:CDS |
| ATGGTGAAATCAATCACTGT+TGG | 0.663372 | 7:+81936276 | None:intergenic |
| TCGGATATTTAAGATGAAGG+TGG | 0.674233 | 7:-81936325 | MsaT038882.1:CDS |
| AACAGTGATTGATTTCACCA+TGG | 0.680653 | 7:-81936274 | MsaT038882.1:CDS |
| GAGAATATATGAGAGGAACG+AGG | 0.682363 | 7:-81936736 | MsaT038882.1:CDS |
| AAGCTCTGATGACACAATGG+AGG | 0.685488 | 7:-81936638 | MsaT038882.1:CDS |
| GTTTACTGGAGAATGCAAGG+AGG | 0.781160 | 7:-81936666 | MsaT038882.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 81936236 | 81937243 | 81936236 | ID=MsaG038882 |
| Chr7 | mRNA | 81936236 | 81937243 | 81936236 | ID=MsaT038882.1;Parent=MsaG038882 |
| Chr7 | exon | 81936236 | 81936370 | 81936236 | ID=MsaT038882.1.exon4;Parent=MsaT038882.1 |
| Chr7 | CDS | 81936236 | 81936370 | 81936236 | ID=cds.MsaT038882.1;Parent=MsaT038882.1 |
| Chr7 | exon | 81936596 | 81936858 | 81936596 | ID=MsaT038882.1.exon3;Parent=MsaT038882.1 |
| Chr7 | CDS | 81936596 | 81936858 | 81936596 | ID=cds.MsaT038882.1;Parent=MsaT038882.1 |
| Chr7 | exon | 81937051 | 81937092 | 81937051 | ID=MsaT038882.1.exon2;Parent=MsaT038882.1 |
| Chr7 | CDS | 81937051 | 81937092 | 81937051 | ID=cds.MsaT038882.1;Parent=MsaT038882.1 |
| Chr7 | exon | 81937201 | 81937243 | 81937201 | ID=MsaT038882.1.exon1;Parent=MsaT038882.1 |
| Chr7 | CDS | 81937201 | 81937243 | 81937201 | ID=cds.MsaT038882.1;Parent=MsaT038882.1 |
| Gene Sequence |
| Protein sequence |
