AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038901


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038901 MtrunA17_Chr7g0251891 98.990 198 2 0 94 291 7 204 5.35e-148 413
MsaG038901 MtrunA17_Chr7g0251881 83.333 108 8 3 1 103 22 124 6.56e-51 164
MsaG038901 MtrunA17_Chr7g0237841 54.054 111 51 0 98 208 618 728 2.10e-33 129
MsaG038901 MtrunA17_Chr2g0281191 39.394 132 71 3 103 229 352 479 1.01e-20 92.4
MsaG038901 MtrunA17_Chr4g0012861 39.806 103 61 1 103 205 314 415 7.37e-20 89.7
MsaG038901 MtrunA17_Chr5g0432721 49.412 85 42 1 103 187 321 404 7.60e-20 89.7
MsaG038901 MtrunA17_Chr3g0107701 40.777 103 60 1 103 205 338 439 9.70e-20 89.4
MsaG038901 MtrunA17_Chr4g0047031 33.333 171 95 5 45 207 120 279 1.00e-19 87.8
MsaG038901 MtrunA17_Chr7g0244871 43.182 88 49 1 103 190 12 98 6.23e-19 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038901 AT3G26790.1 51.634 306 95 12 23 291 24 313 1.73e-87 264
MsaG038901 AT3G24650.1 39.352 216 73 6 45 204 459 672 6.54e-36 136
MsaG038901 AT1G28300.1 49.606 127 64 0 95 221 162 288 9.60e-35 129
MsaG038901 AT2G30470.1 39.806 103 61 1 103 205 294 395 5.02e-21 93.6
MsaG038901 AT4G32010.2 34.104 173 98 5 103 272 281 440 1.07e-20 92.4
MsaG038901 AT4G32010.1 34.104 173 98 5 103 272 285 444 1.08e-20 92.4
MsaG038901 AT4G21550.2 38.835 103 60 2 103 205 327 426 4.54e-17 81.6
MsaG038901 AT4G21550.1 38.835 103 60 2 103 205 327 426 4.85e-17 81.6
MsaG038901 AT4G21550.3 38.835 103 60 2 103 205 327 426 5.09e-17 81.3
MsaG038901 AT1G13260.1 35.897 117 67 5 98 209 182 295 5.04e-11 62.8

Find 51 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTGTTCTTCAACTCCTTT+TGG 0.172423 7:-82159067 None:intergenic
TGATCCAATTCTTGAGTAAT+TGG 0.227933 7:-82160133 None:intergenic
ATTATCTTGGTAAACCATAA+TGG 0.319330 7:-82159775 None:intergenic
GGTTTAGTTGCTGCTGCATT+TGG 0.326686 7:+82157044 MsaT038901.1:CDS
ATTGGATCAGTTGAAACATT+TGG 0.328924 7:+82160147 MsaT038901.1:CDS
TACATTCGACTGTTATTGTT+AGG 0.354796 7:-82159607 None:intergenic
TGCATTACTACAGGTAATTC+AGG 0.355023 7:+82159912 MsaT038901.1:intron
ATGACCAATTACTCAAGAAT+TGG 0.363460 7:+82160129 MsaT038901.1:CDS
ACGCTTTGGAGACTCCATTA+TGG 0.373590 7:+82159761 MsaT038901.1:CDS
GAATTCCTTCCTTCGATTCA+AGG 0.374741 7:-82159270 None:intergenic
CTGCATTTGGAACTGTTCAT+AGG 0.379357 7:+82157057 MsaT038901.1:CDS
AACAACAACAACAAGATTAA+CGG 0.429296 7:+82156993 MsaT038901.1:CDS
AGCACACATGGTCTACGCTT+TGG 0.436188 7:+82159747 MsaT038901.1:CDS
TTGTGATCAAGATGAGTATA+TGG 0.437591 7:+82159945 MsaT038901.1:CDS
AGAGGTGGAATTGGAGAGAT+TGG 0.443660 7:-82157176 None:intergenic
CCTTCCTTCGATTCAAGGAC+AGG 0.443951 7:-82159265 None:intergenic
GAACTGTTCATAGGAAGAAA+AGG 0.451838 7:+82157066 MsaT038901.1:CDS
GTTTATAGGAAATCGATCAT+AGG 0.469630 7:+82159027 MsaT038901.1:intron
ATATAGATGGTCTTCATGTA+TGG 0.471743 7:+82159308 MsaT038901.1:CDS
ACAACAACAACAAGATTAAC+GGG 0.472192 7:+82156994 MsaT038901.1:CDS
TATCTTCGTCGATGATTATG+AGG 0.472524 7:+82159993 MsaT038901.1:CDS
TCTCTTGATGACTTCTATTG+AGG 0.486619 7:+82160186 MsaT038901.1:CDS
ATGTATGGAGTTTCAAGTAC+AGG 0.489515 7:+82159323 MsaT038901.1:CDS
GAGGCGTGTGGTTTCATGGC+AGG 0.489847 7:+82156930 None:intergenic
CTCAGCATGAATGATATAGA+TGG 0.492894 7:+82159295 MsaT038901.1:CDS
ATACTTGTATCATTCATTGC+AGG 0.501561 7:-82160045 None:intergenic
CCTTCCTGTCCTTGAATCGA+AGG 0.505006 7:+82159261 MsaT038901.1:CDS
TAGGAGAATGGTGTTGCCTA+AGG 0.511895 7:+82159103 MsaT038901.1:CDS
TAGGCAACACCATTCTCCTA+AGG 0.515359 7:-82159100 None:intergenic
AGATTAACGGGTCGGGTCAA+CGG 0.518369 7:+82157006 MsaT038901.1:CDS
GAGGTAGTAGTAGTAGAAGA+AGG 0.523751 7:-82157146 None:intergenic
TGTTAGCTCCCTTAGGAGAA+TGG 0.536299 7:+82159091 MsaT038901.1:CDS
CAACGGATCCATCATGATCA+TGG 0.538714 7:+82157023 MsaT038901.1:CDS
CTAGGGTTTGTCAACGTGAA+AGG 0.540011 7:+82156966 MsaT038901.1:CDS
GGAGACTTTGTTAGCACACA+TGG 0.544519 7:+82159735 MsaT038901.1:intron
GCAGGTGTTGAAGATGAACT+AGG 0.544873 7:+82156948 MsaT038901.1:CDS
CAACTAAACCATGATCATGA+TGG 0.545398 7:-82157031 None:intergenic
AACAACAAGATTAACGGGTC+GGG 0.549708 7:+82156999 MsaT038901.1:CDS
ATAGGAAGAAAAGGATGGCT+AGG 0.560627 7:+82157075 MsaT038901.1:CDS
CAACAACAAGATTAACGGGT+CGG 0.560654 7:+82156998 MsaT038901.1:CDS
ACAGTGATGTTAGCTCCCTT+AGG 0.583324 7:+82159084 MsaT038901.1:CDS
CAGGTGTTGAAGATGAACTA+GGG 0.583873 7:+82156949 MsaT038901.1:CDS
CCTGTCCTTGAATCGAAGGA+AGG 0.585425 7:+82159265 MsaT038901.1:CDS
TATAGGAAATCGATCATAGG+AGG 0.597683 7:+82159030 MsaT038901.1:intron
AGGCAACACCATTCTCCTAA+GGG 0.607377 7:-82159099 None:intergenic
CCTTCGATTCAAGGACAGGA+AGG 0.620428 7:-82159261 None:intergenic
TGGAGAGATTGGCACGTGCG+AGG 0.625104 7:-82157165 None:intergenic
ATGTATGTACTTGAAAACAC+TGG 0.631186 7:+82159625 MsaT038901.1:CDS
TGTTCATAGGAAGAAAAGGA+TGG 0.632115 7:+82157070 MsaT038901.1:CDS
AAGTGGTGAGTCATTTGAGA+TGG 0.633283 7:-82160088 None:intergenic
TAACTTGCAGAAAGCAGCAG+AGG 0.635910 7:+82159234 MsaT038901.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 82156945 82160206 82156945 ID=MsaG038901
Chr7 mRNA 82156945 82160206 82156945 ID=MsaT038901.1;Parent=MsaG038901
Chr7 exon 82156945 82157223 82156945 ID=MsaT038901.1.exon1;Parent=MsaT038901.1
Chr7 CDS 82156945 82157223 82156945 ID=cds.MsaT038901.1;Parent=MsaT038901.1
Chr7 exon 82159035 82159124 82159035 ID=MsaT038901.1.exon2;Parent=MsaT038901.1
Chr7 CDS 82159035 82159124 82159035 ID=cds.MsaT038901.1;Parent=MsaT038901.1
Chr7 exon 82159244 82159344 82159244 ID=MsaT038901.1.exon3;Parent=MsaT038901.1
Chr7 CDS 82159244 82159344 82159244 ID=cds.MsaT038901.1;Parent=MsaT038901.1
Chr7 exon 82159600 82159646 82159600 ID=MsaT038901.1.exon4;Parent=MsaT038901.1
Chr7 CDS 82159600 82159646 82159600 ID=cds.MsaT038901.1;Parent=MsaT038901.1
Chr7 exon 82159736 82159812 82159736 ID=MsaT038901.1.exon5;Parent=MsaT038901.1
Chr7 CDS 82159736 82159812 82159736 ID=cds.MsaT038901.1;Parent=MsaT038901.1
Chr7 exon 82159925 82160206 82159925 ID=MsaT038901.1.exon6;Parent=MsaT038901.1
Chr7 CDS 82159925 82160206 82159925 ID=cds.MsaT038901.1;Parent=MsaT038901.1
Gene Sequence

>MsaT038901.1

ATGGCAGGTGTTGAAGATGAACTAGGGTTTGTCAACGTGAAAGGAGACAACAACAACAACAAGATTAACGGGTCGGGTCAACGGATCCATCATGATCATGGTTTAGTTGCTGCTGCATTTGGAACTGTTCATAGGAAGAAAAGGATGGCTAGGCAAAGAAGATCTTCATCTTCAACCATCACTATTCATCTCAAAAATCTTCCTTCTTCTACTACTACTACCTCGCACGTGCCAATCTCTCCAATTCCACCTCTTTTTCATTCTCTCCCTCCCGCACGTGAAATCGATCATAGGAGGTTGAGATTCCTTTTCCAAAAGGAGTTGAAGAACAGTGATGTTAGCTCCCTTAGGAGAATGGTGTTGCCTAAGAAAGCAGCAGAGGCTTTCCTTCCTGTCCTTGAATCGAAGGAAGGAATTCTTCTCAGCATGAATGATATAGATGGTCTTCATGTATGGAGTTTCAAGTACAGGTTTTGGCCTAACAATAACAGTCGAATGTATGTACTTGAAAACACTGGAGACTTTGTTAGCACACATGGTCTACGCTTTGGAGACTCCATTATGGTTTACCAAGATAATCAAAATCACAATTATGTAATTCAGGCAAAGAAAGCTTGTGATCAAGATGAGTATATGGAAGAAGCTAATGATACAATCAATCATATCTTCGTCGATGATTATGAGGTGAACAAAAGTTGCTTTGATGTAGCTTATCCTGCAATGAATGATACAAGTATGTCATTTATATATGACACAACCATCTCAAATGACTCACCACTTGATTTTTTGGGTGGATCAATGACCAATTACTCAAGAATTGGATCAGTTGAAACATTTGGCTCTGTTGAGAATCTGTCTCTTGATGACTTCTATTGA

Protein sequence

>MsaT038901.1

MAGVEDELGFVNVKGDNNNNKINGSGQRIHHDHGLVAAAFGTVHRKKRMARQRRSSSSTITIHLKNLPSSTTTTSHVPISPIPPLFHSLPPAREIDHRRLRFLFQKELKNSDVSSLRRMVLPKKAAEAFLPVLESKEGILLSMNDIDGLHVWSFKYRFWPNNNSRMYVLENTGDFVSTHGLRFGDSIMVYQDNQNHNYVIQAKKACDQDEYMEEANDTINHIFVDDYEVNKSCFDVAYPAMNDTSMSFIYDTTISNDSPLDFLGGSMTNYSRIGSVETFGSVENLSLDDFY*