AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038924

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038924 MtrunA17_Chr7g0252211 95.822 766 22 4 1 765 1 757 0.0 1499
MsaG038924 MtrunA17_Chr6g0476111 86.890 717 63 6 57 765 1 694 0.0 1118
MsaG038924 MtrunA17_Chr6g0476121 87.421 636 52 5 136 765 30 643 0.0 1084
MsaG038924 MtrunA17_Chr5g0399801 42.557 524 259 14 247 765 603 1089 1.56e-120 388
MsaG038924 MtrunA17_Chr4g0003501 40.691 521 270 11 247 765 472 955 1.74e-117 377
MsaG038924 MtrunA17_Chr4g0003511 39.847 522 273 12 248 765 568 1052 2.59e-112 365
MsaG038924 MtrunA17_Chr7g0255181 38.252 515 273 12 251 761 228 701 2.15e-98 320
MsaG038924 MtrunA17_Chr8g0368901 37.423 489 265 13 261 737 299 758 3.82e-93 307
MsaG038924 MtrunA17_Chr3g0080761 36.914 512 291 12 258 761 173 660 1.34e-92 303
MsaG038924 MtrunA17_Chr8g0369301 38.008 492 252 14 262 735 280 736 9.70e-92 303
MsaG038924 MtrunA17_Chr8g0368911 36.620 497 270 16 253 735 284 749 2.08e-90 300
MsaG038924 MtrunA17_Chr1g0163051 37.271 491 272 13 259 741 189 651 4.26e-89 294
MsaG038924 MtrunA17_Chr8g0354821 33.846 520 297 13 256 761 198 684 5.14e-86 286
MsaG038924 MtrunA17_Chr6g0467421 34.059 505 275 15 249 735 99 563 1.81e-83 276
MsaG038924 MtrunA17_Chr6g0467381 33.861 505 276 15 249 735 99 563 6.55e-83 275
MsaG038924 MtrunA17_Chr6g0467531 34.132 501 280 15 249 735 99 563 8.97e-82 271
MsaG038924 MtrunA17_Chr6g0467401 33.465 508 274 16 249 735 99 563 3.53e-81 270
MsaG038924 MtrunA17_Chr1g0163041 34.469 499 279 15 259 742 106 571 2.73e-73 249
MsaG038924 MtrunA17_Chr3g0094881 33.532 504 273 17 251 735 162 622 4.23e-73 250
MsaG038924 MtrunA17_Chr7g0232811 31.274 518 252 13 265 765 109 539 1.03e-65 228
MsaG038924 MtrunA17_Chr1g0170651 31.341 552 284 16 243 765 61 546 2.39e-64 224
MsaG038924 MtrunA17_Chr2g0306961 31.911 492 253 15 249 735 48 462 1.92e-62 216
MsaG038924 MtrunA17_Chr7g0232841 30.636 519 240 15 265 765 112 528 1.87e-60 213
MsaG038924 MtrunA17_Chr7g0232981 30.801 487 242 9 279 765 125 516 6.33e-59 208
MsaG038924 MtrunA17_Chr6g0467411 29.505 505 277 17 249 735 38 481 1.71e-56 201
MsaG038924 MtrunA17_Chr5g0403241 28.875 329 179 9 464 765 1207 1507 6.59e-29 124
MsaG038924 MtrunA17_Chr7g0232751 50.000 96 48 0 670 765 24 119 1.12e-27 108
MsaG038924 MtrunA17_Chr1g0183321 28.529 340 170 9 468 761 430 742 1.17e-25 113
MsaG038924 MtrunA17_Chr4g0075321 35.268 224 114 8 545 765 869 1064 2.32e-25 112
MsaG038924 MtrunA17_Chr1g0190311 33.803 213 117 8 553 763 452 642 1.87e-24 108
MsaG038924 MtrunA17_Chr8g0373821 28.667 300 157 10 464 736 248 517 4.79e-23 103
MsaG038924 MtrunA17_Chr7g0262001 29.240 342 165 15 467 761 387 698 7.94e-21 97.8
MsaG038924 MtrunA17_Chr7g0234361 37.600 125 69 2 423 547 224 339 9.75e-21 94.7
MsaG038924 MtrunA17_Chr5g0448331 34.555 191 106 6 286 470 267 444 2.53e-19 92.8
MsaG038924 MtrunA17_Chr8g0389931 33.508 191 108 6 286 470 261 438 1.60e-17 87.4
MsaG038924 MtrunA17_Chr1g0201621 30.198 202 101 7 564 764 61 223 3.37e-12 69.7
MsaG038924 MtrunA17_Chr3g0143561 34.454 119 70 3 331 447 221 333 5.39e-12 68.6
MsaG038924 MtrunA17_Chr1g0201311 29.703 202 102 7 564 764 61 223 1.64e-11 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038924 AT5G13960.1 65.430 512 169 2 254 765 121 624 0.0 731
MsaG038924 AT5G13960.2 65.863 498 162 2 268 765 1 490 0.0 712
MsaG038924 AT2G35160.5 35.599 618 280 16 159 765 248 758 1.28e-107 346
MsaG038924 AT2G35160.2 35.599 618 280 16 159 765 284 794 3.30e-107 346
MsaG038924 AT2G35160.3 35.599 618 280 16 159 765 284 794 3.30e-107 346
MsaG038924 AT2G35160.1 35.599 618 280 16 159 765 284 794 3.30e-107 346
MsaG038924 AT2G35160.4 35.599 618 280 16 159 765 284 794 3.30e-107 346
MsaG038924 AT2G22740.2 38.993 536 273 16 247 765 292 790 4.21e-106 343
MsaG038924 AT2G22740.1 38.993 536 273 16 247 765 292 790 4.21e-106 343
MsaG038924 AT1G73100.1 36.417 508 283 11 263 761 189 665 3.68e-94 308
MsaG038924 AT5G04940.2 35.490 510 293 13 258 761 187 666 6.58e-91 299
MsaG038924 AT5G04940.1 35.490 510 293 13 258 761 187 666 6.58e-91 299
MsaG038924 AT2G33290.1 37.374 495 267 11 254 737 178 640 2.93e-90 297
MsaG038924 AT2G33290.3 37.374 495 267 11 254 737 178 640 2.93e-90 297
MsaG038924 AT2G33290.4 37.374 495 267 11 254 737 178 640 2.93e-90 297
MsaG038924 AT2G33290.2 37.374 495 267 11 254 737 178 640 2.93e-90 297
MsaG038924 AT4G13460.1 38.217 471 253 13 278 739 200 641 4.66e-90 296
MsaG038924 AT4G13460.2 38.217 471 253 13 278 739 200 641 4.66e-90 296
MsaG038924 AT1G17770.1 33.066 499 285 14 279 761 224 689 2.13e-69 242
MsaG038924 AT2G24740.1 32.863 496 269 15 279 761 307 751 8.82e-59 213
MsaG038924 AT5G47150.1 40.741 189 101 4 256 443 150 328 6.92e-32 127
MsaG038924 AT2G23740.1 29.358 327 173 11 463 764 1081 1374 3.31e-27 119
MsaG038924 AT2G23740.3 29.358 327 173 11 463 764 1088 1381 4.27e-27 119
MsaG038924 AT2G23740.6 29.358 327 173 11 463 764 1088 1381 4.27e-27 119
MsaG038924 AT2G23740.2 29.358 327 173 11 463 764 1088 1381 4.27e-27 119
MsaG038924 AT5G47160.1 42.138 159 80 4 279 435 259 407 8.84e-27 114
MsaG038924 AT5G47160.3 42.138 159 80 4 279 435 259 407 8.84e-27 114
MsaG038924 AT5G47160.2 42.138 159 80 4 279 435 259 407 8.84e-27 114
MsaG038924 AT3G04380.2 26.239 343 178 11 468 763 119 433 9.39e-23 102
MsaG038924 AT3G04380.3 26.239 343 178 11 468 763 130 444 9.50e-23 102
MsaG038924 AT3G04380.1 26.239 343 178 11 468 763 146 460 9.84e-23 103
MsaG038924 AT3G04380.4 26.239 343 178 11 468 763 146 460 1.31e-22 102
MsaG038924 AT3G04380.6 26.239 343 178 11 468 763 119 433 1.39e-22 102
MsaG038924 AT3G04380.5 26.239 343 178 11 468 763 103 417 1.87e-22 101
MsaG038924 AT3G03750.2 29.644 253 123 8 521 764 143 349 1.18e-21 97.8
MsaG038924 AT1G04050.2 26.176 340 180 10 468 763 409 721 3.98e-21 99.4
MsaG038924 AT1G04050.1 26.176 340 180 10 468 763 409 721 3.98e-21 99.4
MsaG038924 AT1G04050.3 26.176 340 180 10 468 763 305 617 5.19e-21 98.6
MsaG038924 AT1G57820.1 35.106 188 95 8 274 445 256 432 4.16e-19 92.4
MsaG038924 AT1G57820.3 35.106 188 95 8 274 445 256 432 4.16e-19 92.4
MsaG038924 AT2G23740.4 28.516 256 129 9 463 693 1088 1314 3.50e-18 90.5
MsaG038924 AT2G23740.5 28.516 256 129 9 463 693 1088 1314 3.50e-18 90.5
MsaG038924 AT2G05900.1 28.821 229 143 7 299 525 1 211 1.12e-17 85.1
MsaG038924 AT1G57820.2 34.043 188 94 9 274 445 256 429 3.78e-16 83.2
MsaG038924 AT5G39550.1 31.383 188 102 7 274 445 241 417 6.28e-16 82.4
MsaG038924 AT1G66050.1 30.319 188 104 7 274 445 241 417 9.91e-15 78.6
MsaG038924 AT1G66040.1 30.319 188 104 7 274 445 241 417 1.03e-14 78.6
MsaG038924 AT1G57800.2 32.105 190 99 8 274 445 268 445 3.64e-13 73.6
MsaG038924 AT1G57800.1 31.746 189 97 8 274 445 268 441 6.34e-13 72.8

Find 196 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCATTCCAGCTACAAATT+TGG 0.221314 7:-82415611 MsaT038924.1:CDS
TTTCATAGCCACTGGTTAAA+TGG 0.230376 7:-82418684 MsaT038924.1:CDS
GCTGTGGCCATTGTTATTTC+AGG 0.244382 7:-82418420 MsaT038924.1:CDS
ACACATTGTACAAACAAATT+AGG 0.303501 7:+82413606 None:intergenic
ATAACAATGGCCACAGCAAC+TGG 0.313591 7:+82418426 None:intergenic
TGCAGAGCGCTTTACCTTAT+GGG 0.314201 7:+82419786 None:intergenic
ACTTCTCCTCACACGCTTCC+TGG 0.326358 7:+82420600 None:intergenic
TAAAATGTGTTACCTGTAAA+GGG 0.331417 7:+82413520 None:intergenic
AAAAGAATTGGCAGCATTCC+AGG 0.338846 7:-82418943 MsaT038924.1:intron
AGCATATGCTTCAAGTTTGC+TGG 0.340531 7:-82413727 MsaT038924.1:CDS
AAGAAGTTGAATTACCGTTT+TGG 0.344471 7:+82418635 None:intergenic
CTGGCGCACTTTCAGAATCT+TGG 0.360036 7:+82413691 None:intergenic
TGACCTCGAATCACCCTTAC+TGG 0.360367 7:+82416909 None:intergenic
GAATCCTAAGGTGAAGATTA+AGG 0.360464 7:-82420827 MsaT038924.1:CDS
AAGCTTCCTGCGAGCGCCTC+TGG 0.360842 7:-82414414 MsaT038924.1:CDS
ACCTTATGGGACTTCTTCCC+TGG 0.363058 7:+82419799 None:intergenic
CTAACAGATGATGCAAAATA+AGG 0.365702 7:-82418983 MsaT038924.1:intron
CGAAGGTGAAAGAGACTATT+AGG 0.366644 7:-82420372 MsaT038924.1:CDS
TGCATTGATGCAGGGTCTAC+TGG 0.368143 7:-82413663 MsaT038924.1:CDS
TAACAAACACTATCTTCATT+TGG 0.375589 7:-82420344 MsaT038924.1:CDS
TTGGTTATGGCTTTCAGATC+AGG 0.378299 7:+82419320 None:intergenic
ACACTATCTTCATTTGGTTC+AGG 0.382382 7:-82420338 MsaT038924.1:intron
TGGGCAGAGAAGGGAATATC+TGG 0.389774 7:-82416391 MsaT038924.1:CDS
AAGGAAGAGGTTGTTGTTGA+AGG 0.393458 7:-82420520 MsaT038924.1:CDS
ATAAAATGTGTTACCTGTAA+AGG 0.394581 7:+82413519 None:intergenic
GTTCTATTCTCGTGCTGAAA+TGG 0.396061 7:-82418718 MsaT038924.1:CDS
TTTGCACATTCACACGTGTT+TGG 0.399670 7:+82414363 None:intergenic
ATTGAAACCTTCAAAAGCTT+CGG 0.400201 7:+82420850 None:intergenic
GCCTACATTAACAACAAATC+AGG 0.403074 7:-82416326 MsaT038924.1:intron
CTCAGGGCCACAGCCACAAT+TGG 0.407383 7:+82414186 None:intergenic
GGAGGTTTGGAATGAGAAAA+TGG 0.407704 7:-82420446 MsaT038924.1:CDS
CTATACCCTGACAAAAGAAT+TGG 0.409301 7:-82418955 MsaT038924.1:CDS
TTTGCAGGTTGACAGCTATT+GGG 0.415977 7:-82416410 MsaT038924.1:intron
ACCAAATTTGTAGCTGGAAT+GGG 0.419060 7:+82415610 None:intergenic
TAGTCGGGAGAGTGTGGTTA+AGG 0.422484 7:-82420575 MsaT038924.1:CDS
GAACTCAATTGTAGAACTGC+TGG 0.424793 7:-82416944 MsaT038924.1:intron
AACGTGAGCTGATGGAGGTT+TGG 0.428185 7:-82420459 MsaT038924.1:CDS
AGCAACTGGGAGTTTGAGCT+TGG 0.434619 7:+82418440 None:intergenic
AACCACAAATTCAACTTGCC+AGG 0.440439 7:+82415562 None:intergenic
AAGGATGGGCTGTTAGATCC+TGG 0.443096 7:-82414013 MsaT038924.1:CDS
CCCCTGGCAAGTTGAATTTG+TGG 0.443703 7:-82415564 MsaT038924.1:CDS
CAGGAGCTGACATATGACTA+TGG 0.448312 7:-82413156 MsaT038924.1:CDS
AATCAAGGGGCTTGGTCAAA+GGG 0.449293 7:-82413874 MsaT038924.1:CDS
CAATCAAGGGGCTTGGTCAA+AGG 0.454994 7:-82413875 MsaT038924.1:CDS
AGGCATGTATGAGGATGATC+TGG 0.456593 7:-82418400 MsaT038924.1:CDS
TTCACCTTAATCTTCACCTT+AGG 0.458201 7:+82420823 None:intergenic
GGGATCAAAAGGTGCCAGTA+AGG 0.458622 7:-82416923 MsaT038924.1:CDS
AGGAAGAGGTTGTTGTTGAA+GGG 0.460266 7:-82420519 MsaT038924.1:CDS
AACCAAATTTGTAGCTGGAA+TGG 0.461157 7:+82415609 None:intergenic
TTACAGGTTTATTTCACAAT+GGG 0.463618 7:-82415786 MsaT038924.1:intron
TCAAATTAATGATCAAAAGT+TGG 0.464564 7:-82418316 MsaT038924.1:CDS
CAGTATACAAGTTTCGTCTC+AGG 0.464923 7:-82416363 MsaT038924.1:CDS
TGTGAGTACACAGGAATACT+TGG 0.466484 7:-82413963 MsaT038924.1:CDS
ATTCCTGTGTACTCACAAAC+TGG 0.467217 7:+82413969 None:intergenic
TTGCAAACAATCAAGGGGCT+TGG 0.470337 7:-82413882 MsaT038924.1:CDS
GGATGAGAAACGTGAGCTGA+TGG 0.472568 7:-82420467 MsaT038924.1:CDS
CGTAAAGTCTATACATATGA+TGG 0.473585 7:-82416864 MsaT038924.1:CDS
GAAAGAGACGACTGAGAAGA+AGG 0.474157 7:-82420539 MsaT038924.1:CDS
AAGCAATTGGCGTGCTATTG+TGG 0.474880 7:-82413093 MsaT038924.1:CDS
GTAGAACTGCTGGGATCAAA+AGG 0.476423 7:-82416934 MsaT038924.1:intron
TTTCGTCTCAGGAGGGTTGA+AGG 0.477074 7:-82416352 MsaT038924.1:CDS
CGGCCAATCTTATTCCAAAA+CGG 0.477129 7:-82418649 MsaT038924.1:intron
AAGAGGACTTCAATATCGTC+TGG 0.478541 7:-82414143 MsaT038924.1:CDS
TGTTATTTCAGGCATGTATG+AGG 0.478696 7:-82418409 MsaT038924.1:CDS
TGATGGGAAGATAAAGCAAT+TGG 0.479828 7:-82413106 MsaT038924.1:CDS
CCCATCCTTTCTTTGCAGTA+CGG 0.480931 7:+82414027 None:intergenic
TGAAGACGTGGACAGTGTAT+TGG 0.482919 7:-82413934 MsaT038924.1:CDS
ACCTGATTTGTTGTTAATGT+AGG 0.483738 7:+82416325 None:intergenic
AGGTTTCGCCGGTGAAGTCC+AGG 0.486360 7:-82420618 MsaT038924.1:CDS
GAAGGTTTCAATGAATCCTA+AGG 0.487492 7:-82420839 MsaT038924.1:CDS
AGATTGCTTGCAAACAATCA+AGG 0.487602 7:-82413889 MsaT038924.1:CDS
GCGTGCTATTGTGGAGCTAC+AGG 0.488774 7:-82413084 MsaT038924.1:CDS
TAATGATCAAAAGTTGGAGC+GGG 0.489455 7:-82418310 MsaT038924.1:CDS
TTGATTGAAGGCATTGAGGT+TGG 0.491065 7:-82418747 MsaT038924.1:intron
GCAACTGGGAGTTTGAGCTT+GGG 0.491570 7:+82418441 None:intergenic
GCAGATTTCTTCTTCTTCGT+CGG 0.491844 7:+82420493 None:intergenic
GTGGCCCTGAGTGTGTGAAT+AGG 0.492031 7:-82414174 MsaT038924.1:CDS
AGAGAAATCCGACGAGGTTG+AGG 0.493999 7:-82420638 MsaT038924.1:CDS
GTCTTTGCATTTGCATCCAG+AGG 0.498540 7:+82414398 None:intergenic
TGACAGCTATTGGGCAGAGA+AGG 0.501325 7:-82416401 MsaT038924.1:CDS
CAAATATTTGAAGTCTCTGA+AGG 0.505529 7:-82414451 MsaT038924.1:intron
GTGAAGATTAAGGTGAAATA+CGG 0.506723 7:-82420817 MsaT038924.1:CDS
TTGAAGAGATTCAGCTTCGC+CGG 0.509201 7:-82420684 MsaT038924.1:CDS
TCAGGGCCACAGCCACAATT+GGG 0.510052 7:+82414187 None:intergenic
TTTCCCTATGTATCTAAAGA+TGG 0.511890 7:-82414324 MsaT038924.1:CDS
TCCGTACTGCAAAGAAAGGA+TGG 0.513656 7:-82414028 MsaT038924.1:CDS
TGATGATATCACTAACGGTC+AGG 0.516885 7:-82415641 MsaT038924.1:CDS
TTGCATCCAGAGGCGCTCGC+AGG 0.517133 7:+82414408 None:intergenic
CGTGCTGAAATGGTTGCAGT+TGG 0.517795 7:-82418708 MsaT038924.1:CDS
TGTGGTCCCAATTGTGGCTG+TGG 0.518256 7:-82414193 MsaT038924.1:CDS
CTCGAGCCAATCGCAAATCA+TGG 0.522922 7:+82413571 None:intergenic
TTTGAATGTGGTCCCAATTG+TGG 0.523304 7:-82414199 MsaT038924.1:CDS
GCCACCATCTTTAGATACAT+AGG 0.523676 7:+82414320 None:intergenic
AACTCAATTGTAGAACTGCT+GGG 0.525146 7:-82416943 MsaT038924.1:intron
TGTGAGGAGAAGTCCTAGTC+GGG 0.527597 7:-82420590 MsaT038924.1:CDS
TCATCAACCAAATTTGTAGC+TGG 0.528022 7:+82415604 None:intergenic
CTATGCACTTGATAGTGTCT+TGG 0.529289 7:-82413133 MsaT038924.1:CDS
ATTGTGGAGCTACAGGCTGC+AGG 0.531984 7:-82413077 MsaT038924.1:CDS
GGCTTTCAGATCAGGTCGCT+TGG 0.533756 7:+82419328 None:intergenic
TCCAGGGAAGAAGTCCCATA+AGG 0.534829 7:-82419800 MsaT038924.1:intron
TCAGATGGTTGCGCAATCGT+TGG 0.536360 7:-82420908 None:intergenic
CCTGCATCAATGCAAAACTC+TGG 0.537698 7:+82413672 None:intergenic
GATAGTGTCTTGGACTCTGA+TGG 0.539422 7:-82413123 MsaT038924.1:CDS
GAAATTGAGAAGAGAGGTGG+CGG 0.540940 7:-82419892 MsaT038924.1:CDS
CACACACCATGATTTGCGAT+TGG 0.545362 7:-82413577 MsaT038924.1:CDS
CCACCATCTTTAGATACATA+GGG 0.545827 7:+82414321 None:intergenic
TCTCTTGATTGAAGGCATTG+AGG 0.547725 7:-82418751 MsaT038924.1:intron
TGTACAGGAGGAGGAGGCGA+GGG 0.548368 7:-82419932 MsaT038924.1:intron
TACATATGATGGCTTGTACA+AGG 0.548494 7:-82416853 MsaT038924.1:intron
CAGCTTCGCCGGAAAGCTGA+GGG 0.548569 7:-82420673 MsaT038924.1:CDS
AGGATGGGCTGTTAGATCCT+GGG 0.549307 7:-82414012 MsaT038924.1:CDS
CGCAGAGGAGATCGAGTTCG+AGG 0.550863 7:-82420729 MsaT038924.1:CDS
GATTGCTTGCAAACAATCAA+GGG 0.551561 7:-82413888 MsaT038924.1:CDS
TGATAAGAGCTCAACGGCGA+AGG 0.555781 7:-82420389 MsaT038924.1:CDS
GAAGTCCTATTCACACACTC+AGG 0.559130 7:+82414169 None:intergenic
ATGTATCTAAAGATGGTGGC+AGG 0.561395 7:-82414317 MsaT038924.1:intron
ACCACAAATTCAACTTGCCA+GGG 0.561590 7:+82415563 None:intergenic
CAAGGTGGGCATAATCTGAC+TGG 0.563101 7:-82418348 MsaT038924.1:CDS
GAAGCTGAAAGATGAATCGA+AGG 0.565258 7:-82420788 MsaT038924.1:CDS
TTAACCACACTCTCCCGACT+AGG 0.568352 7:+82420577 None:intergenic
ACAAATTAGGCTCACAACAA+TGG 0.568537 7:+82413619 None:intergenic
GCGGGGTAATTTAGCACTCA+AGG 0.570642 7:-82418292 MsaT038924.1:intron
ATTACAGGTTTATTTCACAA+TGG 0.571455 7:-82415787 MsaT038924.1:intron
AATGCTGAAATTGAGAAGAG+AGG 0.574775 7:-82419898 MsaT038924.1:CDS
CGACGAGGTTGAGGTTTCGC+CGG 0.575414 7:-82420629 MsaT038924.1:CDS
GCTGCTGAAACTGAAAATGC+TGG 0.575951 7:-82420424 MsaT038924.1:CDS
GACGACTGAGAAGAAGGAAG+AGG 0.576234 7:-82420533 MsaT038924.1:CDS
ACCGGAAGGTATAAAATCCC+AGG 0.577194 7:+82413995 None:intergenic
GATCTCCTCTGCGACGACAT+TGG 0.578970 7:+82420739 None:intergenic
TATACAAGTTTCGTCTCAGG+AGG 0.580091 7:-82416360 MsaT038924.1:CDS
ATGTACAGGAGGAGGAGGCG+AGG 0.581181 7:-82419933 MsaT038924.1:intron
TTGGAATGTACAGGAGGAGG+AGG 0.583067 7:-82419938 MsaT038924.1:intron
GACAGCTATTGGGCAGAGAA+GGG 0.583693 7:-82416400 MsaT038924.1:CDS
CCGTACTGCAAAGAAAGGAT+GGG 0.585335 7:-82414027 MsaT038924.1:CDS
ACACGCTTCCTGGACTTCAC+CGG 0.585913 7:+82420610 None:intergenic
GTACTCACAAACTGGTGCAC+CGG 0.586682 7:+82413977 None:intergenic
GCTCAAACTCCCAGTTGCTG+TGG 0.587054 7:-82418436 MsaT038924.1:CDS
CAACTGGGAGTTTGAGCTTG+GGG 0.587889 7:+82418442 None:intergenic
GATTCAAGCTAAGCAGAAAG+AGG 0.589427 7:-82420707 MsaT038924.1:CDS
ACCAGTGTACACAACATCCT+CGG 0.591147 7:+82418371 None:intergenic
AAGTCCTAGTCGGGAGAGTG+TGG 0.593196 7:-82420581 MsaT038924.1:CDS
GGATCAAAAGGTGCCAGTAA+GGG 0.593584 7:-82416922 MsaT038924.1:CDS
GTGTGAGGAGAAGTCCTAGT+CGG 0.593704 7:-82420591 MsaT038924.1:CDS
TCAGCTTCGCCGGAAAGCTG+AGG 0.594733 7:-82420674 MsaT038924.1:CDS
AGTACACAGGAATACTTGGT+AGG 0.609435 7:-82413959 MsaT038924.1:CDS
CGTATTTCTGCTGTAGACTG+AGG 0.611269 7:+82415756 None:intergenic
TGAAGTCCAGGAAGCGTGTG+AGG 0.611851 7:-82420606 MsaT038924.1:CDS
TCGGGAGAGTGTGGTTAAGG+AGG 0.611887 7:-82420572 MsaT038924.1:CDS
AAAATGTGTTACCTGTAAAG+GGG 0.612555 7:+82413521 None:intergenic
CCCTATGTATCTAAAGATGG+TGG 0.617550 7:-82414321 MsaT038924.1:CDS
AAGATTAAGGTGAAATACGG+TGG 0.617774 7:-82420814 MsaT038924.1:CDS
ATATGTGATGATATCACTAA+CGG 0.618325 7:-82415646 MsaT038924.1:CDS
ATAGTGTCTTGGACTCTGAT+GGG 0.618534 7:-82413122 MsaT038924.1:CDS
AGATGAATCGAAGGATGCGA+CGG 0.619609 7:-82420779 MsaT038924.1:CDS
TTAATGATCAAAAGTTGGAG+CGG 0.620986 7:-82418311 MsaT038924.1:CDS
GCTTCGCCGGAAAGCTGAGG+GGG 0.621254 7:-82420671 MsaT038924.1:CDS
GTGCCAGTAAGGGTGATTCG+AGG 0.621922 7:-82416912 MsaT038924.1:CDS
AATCAATGCCATTTAACCAG+TGG 0.631469 7:+82418676 None:intergenic
AAATGTGTTACCTGTAAAGG+GGG 0.632493 7:+82413522 None:intergenic
TAACAATGGCCACAGCAACT+GGG 0.637216 7:+82418427 None:intergenic
ATACATGCCTGAAATAACAA+TGG 0.637494 7:+82418413 None:intergenic
GCCGAGGATGTTGTGTACAC+TGG 0.638983 7:-82418372 MsaT038924.1:CDS
GAAGAAGAAGAAATCTGCGT+CGG 0.644216 7:-82420488 MsaT038924.1:CDS
CGGCGAAACCTCAACCTCGT+CGG 0.649190 7:+82420630 None:intergenic
CACAAATTCAACTTGCCAGG+GGG 0.649975 7:+82415565 None:intergenic
CAAGGGGCTTGGTCAAAGGG+AGG 0.650879 7:-82413871 MsaT038924.1:intron
TCACAAACTGGTGCACCGGA+AGG 0.652054 7:+82413981 None:intergenic
GAAGGATGCGACGGTGACGA+CGG 0.653424 7:-82420770 MsaT038924.1:CDS
TGAGAAACGTGAGCTGATGG+AGG 0.654715 7:-82420464 MsaT038924.1:CDS
ATTGCTTGCAAACAATCAAG+GGG 0.655499 7:-82413887 MsaT038924.1:CDS
AACAACACAATCATTCGCAA+CGG 0.656487 7:+82420873 None:intergenic
CAGTCTACAGCAGAAATACG+AGG 0.656834 7:-82415753 MsaT038924.1:CDS
GATGTTGTGTACACTGGTCA+AGG 0.657319 7:-82418366 MsaT038924.1:CDS
TTGTGTACACTGGTCAAGGT+GGG 0.660354 7:-82418362 MsaT038924.1:CDS
AAGTCCTATTCACACACTCA+GGG 0.661425 7:+82414170 None:intergenic
ACTTGGTAGGACTGAAGACG+TGG 0.661663 7:-82413946 MsaT038924.1:CDS
GTTGTGTACACTGGTCAAGG+TGG 0.663096 7:-82418363 MsaT038924.1:CDS
GGATGCGACGGTGACGACGG+AGG 0.664620 7:-82420767 MsaT038924.1:CDS
GCTGAAACTGAAAATGCTGG+TGG 0.670068 7:-82420421 MsaT038924.1:CDS
AATGATCAAAAGTTGGAGCG+GGG 0.670495 7:-82418309 MsaT038924.1:CDS
AAATTGAGAAGAGAGGTGGC+GGG 0.671120 7:-82419891 MsaT038924.1:CDS
GCTGAAATTGAGAAGAGAGG+TGG 0.673560 7:-82419895 MsaT038924.1:CDS
AAAGACGTTGTGTTTGAATG+TGG 0.675624 7:-82414211 MsaT038924.1:CDS
TGACACCAATGTCGTCGCAG+AGG 0.677701 7:-82420744 MsaT038924.1:CDS
TGTGAATGTGCAAAGCGCAA+TGG 0.684582 7:-82414354 MsaT038924.1:CDS
ATACAAGTTTCGTCTCAGGA+GGG 0.687263 7:-82416359 MsaT038924.1:CDS
AGGACTTCAATATCGTCTGG+AGG 0.687370 7:-82414140 MsaT038924.1:intron
ATTGAGTGATAAGAGCTCAA+CGG 0.696173 7:-82420395 MsaT038924.1:CDS
GCACCAGTTTGTGAGTACAC+AGG 0.696684 7:-82413972 MsaT038924.1:CDS
GTGAATAGGACTTCTCAAAG+AGG 0.697957 7:-82414160 MsaT038924.1:CDS
TGATCTGAAAGCCATAACCA+AGG 0.704164 7:-82419318 MsaT038924.1:intron
CCACAAATTCAACTTGCCAG+GGG 0.704891 7:+82415564 None:intergenic
AGCTTCGCCGGAAAGCTGAG+GGG 0.705302 7:-82420672 MsaT038924.1:CDS
AGTCTACAGCAGAAATACGA+GGG 0.715033 7:-82415752 MsaT038924.1:intron
GGATGATCTGGATAATGCCG+AGG 0.717934 7:-82418388 MsaT038924.1:CDS
AGCTGAAGAGAAATCCGACG+AGG 0.742452 7:-82420644 MsaT038924.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 82413062 82420926 82413062 ID=MsaG038924
Chr7 mRNA 82413062 82420926 82413062 ID=MsaT038924.1;Parent=MsaG038924
Chr7 exon 82413062 82413175 82413062 ID=MsaT038924.1.exon15;Parent=MsaT038924.1
Chr7 CDS 82413062 82413175 82413062 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82413533 82413766 82413533 ID=MsaT038924.1.exon14;Parent=MsaT038924.1
Chr7 CDS 82413533 82413766 82413533 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82413872 82414054 82413872 ID=MsaT038924.1.exon13;Parent=MsaT038924.1
Chr7 CDS 82413872 82414054 82413872 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82414141 82414246 82414141 ID=MsaT038924.1.exon12;Parent=MsaT038924.1
Chr7 CDS 82414141 82414246 82414141 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82414318 82414460 82414318 ID=MsaT038924.1.exon11;Parent=MsaT038924.1
Chr7 CDS 82414318 82414460 82414318 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82415543 82415672 82415543 ID=MsaT038924.1.exon10;Parent=MsaT038924.1
Chr7 CDS 82415543 82415672 82415543 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82415753 82415802 82415753 ID=MsaT038924.1.exon9;Parent=MsaT038924.1
Chr7 CDS 82415753 82415802 82415753 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82416327 82416425 82416327 ID=MsaT038924.1.exon8;Parent=MsaT038924.1
Chr7 CDS 82416327 82416425 82416327 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82416854 82416952 82416854 ID=MsaT038924.1.exon7;Parent=MsaT038924.1
Chr7 CDS 82416854 82416952 82416854 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82418293 82418466 82418293 ID=MsaT038924.1.exon6;Parent=MsaT038924.1
Chr7 CDS 82418293 82418466 82418293 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82418650 82418759 82418650 ID=MsaT038924.1.exon5;Parent=MsaT038924.1
Chr7 CDS 82418650 82418759 82418650 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82418944 82418998 82418944 ID=MsaT038924.1.exon4;Parent=MsaT038924.1
Chr7 CDS 82418944 82418998 82418944 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82419319 82419384 82419319 ID=MsaT038924.1.exon3;Parent=MsaT038924.1
Chr7 CDS 82419319 82419384 82419319 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82419801 82419947 82419801 ID=MsaT038924.1.exon2;Parent=MsaT038924.1
Chr7 CDS 82419801 82419947 82419801 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Chr7 exon 82420339 82420926 82420339 ID=MsaT038924.1.exon1;Parent=MsaT038924.1
Chr7 CDS 82420339 82420926 82420339 ID=cds.MsaT038924.1;Parent=MsaT038924.1
Gene Sequence

>MsaT038924.1

ATGGTTGCGCAATCGTTGGAGATTCGTGATTCCGTTGCGAATGATTGTGTTGTTCCGAAGCTTTTGAAGGTTTCAATGAATCCTAAGGTGAAGATTAAGGTGAAATACGGTGGAAAGAAGCTGAAAGATGAATCGAAGGATGCGACGGTGACGACGGAGGTGACACCAATGTCGTCGCAGAGGAGATCGAGTTCGAGGATTCAAGCTAAGCAGAAAGAGGTTGAAGAGATTCAGCTTCGCCGGAAAGCTGAGGGGGTAGAAGCTGAAGAGAAATCCGACGAGGTTGAGGTTTCGCCGGTGAAGTCCAGGAAGCGTGTGAGGAGAAGTCCTAGTCGGGAGAGTGTGGTTAAGGAGGTTGTTGAAGAGAAAGAGACGACTGAGAAGAAGGAAGAGGTTGTTGTTGAAGGGAAACCGACGAAGAAGAAGAAATCTGCGTCGGATGAGAAACGTGAGCTGATGGAGGTTTGGAATGAGAAAATGGCTGCTGAAACTGAAAATGCTGGTGGTGCATTGAGTGATAAGAGCTCAACGGCGAAGGTGAAAGAGACTATTAGGACTTTTAACAAACACTATCTTCATTTGGTTCAGGAGGAGGAGGCGAGGGTTGCTAAAGATAATGCTGAAATTGAGAAGAGAGGTGGCGGGAAGAAATCTGCTGCCGATAAGAAAGCTGCTAAAAAGAAATCTCCTGAGAAGAAATCTCCTAAAAAATCTCCAGGGAAGAAGTCCCATAAGAAAGATGCAGAACCTAAAGAAAAACAGCACTCTGCCAAGCGACCTGATCTGAAAGCCATAACCAAGATGATGCAAAATAAGGAGATACTATACCCTGACAAAAGAATTGGCAGCATTCCAGGCATTGAGGTTGGATATCAGTTCTATTCTCGTGCTGAAATGGTTGCAGTTGGTTTTCATAGCCACTGGTTAAATGGCATTGATTTTATCGGCCAATCTTATTCCAAAACGTACCCCAAGCTCAAACTCCCAGTTGCTGTGGCCATTGTTATTTCAGGCATGTATGAGGATGATCTGGATAATGCCGAGGATGTTGTGTACACTGGTCAAGGTGGGCATAATCTGACTGGTAATAAGCGTCAAATTAATGATCAAAAGTTGGAGCGGGGTAATTTAGCACTCAAGAACTGCTGGGATCAAAAGGTGCCAGTAAGGGTGATTCGAGGTCATGACTCACGTAGCAGTTACACTCGTAAAGTCTATACATATGATGGCTTGTACAAGGTTGACAGCTATTGGGCAGAGAAGGGAATATCTGGCTTCACAGTATACAAGTTTCGTCTCAGGAGGGTTGAAGGACAGCCTACATTAACAACAAATCAGGTTTATTTCACAATGGGACGTGTACCTCAGTCTACAGCAGAAATACGAGGGTTGATATGTGATGATATCACTAACGGTCAGGAAGCTGTGCCCATTCCAGCTACAAATTTGGTTGATGACCCACCTGTTCCACCGCCCCCTGGCAAGTTGAATTTGTGGTTTTTGTTTGATTTCCCTGCTTCTCTGAAGGTTGCAAAAAGTGTTAAGCTTCCTGCGAGCGCCTCTGGATGCAAATGCAAAGACAAATGTACTGATCCAAACACGTGTGAATGTGCAAAGCGCAATGGCTCTGAATTTCCCTATGTATCTAAAGATGGTGGCAGATTAATTGAAGCTAAAGACGTTGTGTTTGAATGTGGTCCCAATTGTGGCTGTGGCCCTGAGTGTGTGAATAGGACTTCTCAAAGAGGACTTCAATATCGTCTGGAGGTTTTCCGTACTGCAAAGAAAGGATGGGCTGTTAGATCCTGGGATTTTATACCTTCCGGTGCACCAGTTTGTGAGTACACAGGAATACTTGGTAGGACTGAAGACGTGGACAGTGTATTGGAAAACAACTATATTTTTGAGATAGATTGCTTGCAAACAATCAAGGGGCTTGGTCAAAGGGAGAGGAGGTCAAAAAATGTAGCATATGCTTCAAGTTTGCTGGAAAAATACGATGACCAAGATTCTGAAAGTGCGCCAGAGTTTTGCATTGATGCAGGGTCTACTGGAAATGTTGCTAGATTTATAAACCATTGTTGTGAGCCTAATTTGTTTGTACAATGTGTTTTGAGCACACACCATGATTTGCGATTGGCTCGAGTAGTGCTGTTTGCTGCTGACAACATACCCCCTTTACAGGAGCTGACATATGACTATGGCTATGCACTTGATAGTGTCTTGGACTCTGATGGGAAGATAAAGCAATTGGCGTGCTATTGTGGAGCTACAGGCTGCAGGAAGCGATTATTCTAA
Protein sequence

>MsaT038924.1

MVAQSLEIRDSVANDCVVPKLLKVSMNPKVKIKVKYGGKKLKDESKDATVTTEVTPMSSQRRSSSRIQAKQKEVEEIQLRRKAEGVEAEEKSDEVEVSPVKSRKRVRRSPSRESVVKEVVEEKETTEKKEEVVVEGKPTKKKKSASDEKRELMEVWNEKMAAETENAGGALSDKSSTAKVKETIRTFNKHYLHLVQEEEARVAKDNAEIEKRGGGKKSAADKKAAKKKSPEKKSPKKSPGKKSHKKDAEPKEKQHSAKRPDLKAITKMMQNKEILYPDKRIGSIPGIEVGYQFYSRAEMVAVGFHSHWLNGIDFIGQSYSKTYPKLKLPVAVAIVISGMYEDDLDNAEDVVYTGQGGHNLTGNKRQINDQKLERGNLALKNCWDQKVPVRVIRGHDSRSSYTRKVYTYDGLYKVDSYWAEKGISGFTVYKFRLRRVEGQPTLTTNQVYFTMGRVPQSTAEIRGLICDDITNGQEAVPIPATNLVDDPPVPPPPGKLNLWFLFDFPASLKVAKSVKLPASASGCKCKDKCTDPNTCECAKRNGSEFPYVSKDGGRLIEAKDVVFECGPNCGCGPECVNRTSQRGLQYRLEVFRTAKKGWAVRSWDFIPSGAPVCEYTGILGRTEDVDSVLENNYIFEIDCLQTIKGLGQRERRSKNVAYASSLLEKYDDQDSESAPEFCIDAGSTGNVARFINHCCEPNLFVQCVLSTHHDLRLARVVLFAADNIPPLQELTYDYGYALDSVLDSDGKIKQLACYCGATGCRKRLF*