AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038997

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038997 MtrunA17_Chr7g0253141 99.245 265 0 1 1 265 1 263 0.0 550
MsaG038997 MtrunA17_Chr1g0169261 30.888 259 146 6 9 258 3 237 1.30e-31 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038997 AT1G54390.4 79.623 265 47 4 1 263 1 260 6.21e-143 402
MsaG038997 AT1G54390.3 79.623 265 47 4 1 263 1 260 6.21e-143 402
MsaG038997 AT1G54390.7 76.211 227 47 4 39 263 19 240 1.15e-113 327
MsaG038997 AT1G54390.8 76.211 227 47 4 39 263 19 240 1.15e-113 327
MsaG038997 AT1G54390.5 76.211 227 47 4 39 263 19 240 1.15e-113 327
MsaG038997 AT1G54390.2 77.922 231 44 4 1 229 1 226 5.65e-113 328
MsaG038997 AT1G54390.1 77.922 231 44 4 1 229 1 226 5.93e-113 327
MsaG038997 AT1G54390.6 81.768 181 29 3 85 263 1 179 1.86e-99 288
MsaG038997 AT3G24010.1 36.111 180 99 4 79 258 61 224 8.11e-30 112

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAATTTCAGGTATCTTT+TGG 0.176968 7:+83568292 MsaT038997.1:intron
TAGTCCTTCTTATGGCTTCC+TGG 0.301453 7:-83568098 None:intergenic
AGTCTACGTCGATGACTATT+TGG 0.315508 7:+83566346 MsaT038997.1:CDS
AATCGAAGAGACTTGATTAC+AGG 0.362393 7:+83568036 MsaT038997.1:CDS
ACACAGTATGTAGGTTCATT+TGG 0.368463 7:-83568155 None:intergenic
ACTTTGTCTGCTGCCTTGTC+TGG 0.368542 7:-83566719 None:intergenic
TGTAGGTTCATTTGGATCAA+TGG 0.378225 7:-83568147 None:intergenic
AAAAGAAATCGAGGCGAATC+AGG 0.384479 7:+83566848 MsaT038997.1:CDS
GGATCATATAGTCCTTCTTA+TGG 0.391014 7:-83568106 None:intergenic
GTAGGTTCATTTGGATCAAT+GGG 0.417965 7:-83568146 None:intergenic
TACATACTGTGTTTGTCATC+AGG 0.423437 7:+83568166 MsaT038997.1:CDS
CTTTAGATCTTCAGCGAAGT+AGG 0.427352 7:-83567523 None:intergenic
TTGATTACAGGGAGAGAGAC+TGG 0.434207 7:+83568048 MsaT038997.1:CDS
TTCTTATGGCTTCCTGGTGG+AGG 0.438248 7:-83568092 None:intergenic
AAGCTCAGCGGGCAATGTAT+TGG 0.453107 7:-83566481 None:intergenic
GCAGCAGACAAAGTACTGTA+TGG 0.463935 7:+83566728 MsaT038997.1:CDS
CTCATCTCATGGCTCTAAGA+AGG 0.470707 7:+83566755 MsaT038997.1:CDS
AGAAGTCTCTGAAGCTCAGC+GGG 0.472014 7:-83566492 None:intergenic
GTGGTAGTAATCTGCAAGTT+GGG 0.493544 7:-83568525 None:intergenic
GAAAAGCGCAGGCACGCATA+TGG 0.503955 7:+83568005 MsaT038997.1:CDS
AACACAAGAGACAAGGTTCA+AGG 0.504646 7:+83568489 MsaT038997.1:CDS
TGTATGGGGTTCTCATCTCA+TGG 0.513570 7:+83566744 MsaT038997.1:CDS
TTAGACCAACACATGAATAG+TGG 0.520946 7:-83568468 None:intergenic
GGCACGACAAGCATATGAAC+TGG 0.521248 7:+83566908 MsaT038997.1:CDS
GAAGGACTATATGATCCCTA+TGG 0.521889 7:+83568112 MsaT038997.1:CDS
TGTGGTAGTAATCTGCAAGT+TGG 0.523029 7:-83568526 None:intergenic
GGGATCGTTCATCGAGTTCA+CGG 0.530503 7:-83566524 None:intergenic
CAGCAGACAAAGTACTGTAT+GGG 0.532458 7:+83566729 MsaT038997.1:CDS
ATGGGTTGATCAATATCCAT+AGG 0.533374 7:-83568128 None:intergenic
GATAGTGCCGAGGAGGTGAA+TGG 0.537405 7:+83568443 MsaT038997.1:intron
TAATCAAGTCTCTTCGATTG+AGG 0.541620 7:-83568032 None:intergenic
AAAATGGCAATTGCACGCAC+TGG 0.548966 7:+83566323 None:intergenic
TCCACCAGGAAGCCATAAGA+AGG 0.551349 7:+83568094 MsaT038997.1:CDS
AAGATTTATCATTGACATTG+TGG 0.551966 7:-83568544 None:intergenic
TGGTTCCACTATTCATGTGT+TGG 0.555308 7:+83568463 MsaT038997.1:CDS
ATTGAGTTTATGCACTGAGA+AGG 0.558927 7:+83566878 MsaT038997.1:CDS
ACACAAGAGACAAGGTTCAA+GGG 0.562098 7:+83568490 MsaT038997.1:CDS
AGACAAGGTTCAAGGGAAAG+TGG 0.569480 7:+83568497 MsaT038997.1:CDS
TCCCTAAACCTGAAAAGCGC+AGG 0.571245 7:+83567994 MsaT038997.1:CDS
CAATTACAATTACATGAATG+AGG 0.576780 7:+83566797 MsaT038997.1:CDS
TCCTTCTTATGGCTTCCTGG+TGG 0.577394 7:-83568095 None:intergenic
TTAGGGACAATAGGAAGTGG+GGG 0.580164 7:-83567978 None:intergenic
GGTTTAGGGACAATAGGAAG+TGG 0.584655 7:-83567981 None:intergenic
TGGGTTGATCAATATCCATA+GGG 0.596146 7:-83568127 None:intergenic
TTCGCTGAAGATCTAAAGCA+AGG 0.596239 7:+83567528 MsaT038997.1:CDS
GAGAAGTCTCTGAAGCTCAG+CGG 0.598711 7:-83566493 None:intergenic
TCATCTCATGGCTCTAAGAA+GGG 0.599282 7:+83566756 MsaT038997.1:CDS
TGATGACAAACACAGTATGT+AGG 0.600981 7:-83568164 None:intergenic
GTTTAGGGACAATAGGAAGT+GGG 0.601691 7:-83567980 None:intergenic
TTTGAGCTAATGCCTCCACC+AGG 0.610776 7:+83568080 MsaT038997.1:CDS
CCATGTAAAACGACTTGATG+AGG 0.613217 7:+83567494 MsaT038997.1:CDS
AGTGGAACCATTCACCTCCT+CGG 0.620021 7:-83568450 None:intergenic
TGATTACAGGGAGAGAGACT+GGG 0.622459 7:+83568049 MsaT038997.1:CDS
ATCGAAGAGACTTGATTACA+GGG 0.633832 7:+83568037 MsaT038997.1:CDS
TGTTATTCTTGATAGTGCCG+AGG 0.646877 7:+83568433 MsaT038997.1:intron
TTTAGGGACAATAGGAAGTG+GGG 0.647357 7:-83567979 None:intergenic
AAGCTATGATAAACCAGACA+AGG 0.648028 7:+83566706 MsaT038997.1:intron
TCGTTCATCGAGTTCACGGA+CGG 0.655630 7:-83566520 None:intergenic
AGCAGACAAAGTACTGTATG+GGG 0.659495 7:+83566730 MsaT038997.1:CDS
GAAAATGCGAAAAGAAATCG+AGG 0.660931 7:+83566839 MsaT038997.1:CDS
TATTCTTGATAGTGCCGAGG+AGG 0.680877 7:+83568436 MsaT038997.1:intron
TTGGTCTAACACAAGAGACA+AGG 0.703698 7:+83568482 MsaT038997.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 83566326 83568558 83566326 ID=MsaG038997
Chr7 mRNA 83566326 83568558 83566326 ID=MsaT038997.1;Parent=MsaG038997
Chr7 exon 83566326 83566371 83566326 ID=MsaT038997.1.exon1;Parent=MsaT038997.1
Chr7 CDS 83566326 83566371 83566326 ID=cds.MsaT038997.1;Parent=MsaT038997.1
Chr7 exon 83566478 83566549 83566478 ID=MsaT038997.1.exon2;Parent=MsaT038997.1
Chr7 CDS 83566478 83566549 83566478 ID=cds.MsaT038997.1;Parent=MsaT038997.1
Chr7 exon 83566709 83566929 83566709 ID=MsaT038997.1.exon3;Parent=MsaT038997.1
Chr7 CDS 83566709 83566929 83566709 ID=cds.MsaT038997.1;Parent=MsaT038997.1
Chr7 exon 83567486 83567549 83567486 ID=MsaT038997.1.exon4;Parent=MsaT038997.1
Chr7 CDS 83567486 83567549 83567486 ID=cds.MsaT038997.1;Parent=MsaT038997.1
Chr7 exon 83567943 83568187 83567943 ID=MsaT038997.1.exon5;Parent=MsaT038997.1
Chr7 CDS 83567943 83568187 83567943 ID=cds.MsaT038997.1;Parent=MsaT038997.1
Chr7 exon 83568304 83568342 83568304 ID=MsaT038997.1.exon6;Parent=MsaT038997.1
Chr7 CDS 83568304 83568342 83568304 ID=cds.MsaT038997.1;Parent=MsaT038997.1
Chr7 exon 83568448 83568558 83568448 ID=MsaT038997.1.exon7;Parent=MsaT038997.1
Chr7 CDS 83568448 83568558 83568448 ID=cds.MsaT038997.1;Parent=MsaT038997.1
Gene Sequence

>MsaT038997.1

ATGGCAATTGCACGCACTGGAGTCTACGTCGATGACTATTTGGAGTATGCCAATACATTGCCCGCTGAGCTTCAGAGACTTCTCAACACCGTCCGTGAACTCGATGAACGATCCCAATCTATGATAAACCAGACAAGGCAGCAGACAAAGTACTGTATGGGGTTCTCATCTCATGGCTCTAAGAAGGGTAATCATAATTATAATAACAATTACAATTACATGAATGAGGATGATGATGCTTCCATTGAGAAAATGCGAAAAGAAATCGAGGCGAATCAGGATAGTGCATTGAGTTTATGCACTGAGAAGGTTTTGTTGGCACGACAAGCATATGAACTGATAGACAGCCATGTAAAACGACTTGATGAGGATTTAACCTACTTCGCTGAAGATCTAAAGCAAGAGGGGAAGATATCACAAGACGAGCCAGCAATTCTTCCCCCACTTCCTATTGTCCCTAAACCTGAAAAGCGCAGGCACGCATATGGTACACCTCAATCGAAGAGACTTGATTACAGGGAGAGAGACTGGGATAGAGACTTTGAGCTAATGCCTCCACCAGGAAGCCATAAGAAGGACTATATGATCCCTATGGATATTGATCAACCCATTGATCCAAATGAACCTACATACTGTGTTTGTCATCAGGTATCTTTTGGAGATATGATTGCTTGTGACAACGAAAATTGCCGAGGAGGTGAATGGTTCCACTATTCATGTGTTGGTCTAACACAAGAGACAAGGTTCAAGGGAAAGTGGTATTGCCCAACTTGCAGATTACTACCACAATGTCAATGA
Protein sequence

>MsaT038997.1

MAIARTGVYVDDYLEYANTLPAELQRLLNTVRELDERSQSMINQTRQQTKYCMGFSSHGSKKGNHNYNNNYNYMNEDDDASIEKMRKEIEANQDSALSLCTEKVLLARQAYELIDSHVKRLDEDLTYFAEDLKQEGKISQDEPAILPPLPIVPKPEKRRHAYGTPQSKRLDYRERDWDRDFELMPPPGSHKKDYMIPMDIDQPIDPNEPTYCVCHQVSFGDMIACDNENCRGGEWFHYSCVGLTQETRFKGKWYCPTCRLLPQCQ*