AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG039005

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039005 MtrunA17_Chr7g0253211 97.826 690 12 3 1 688 178 866 0.0 1389
MsaG039005 MtrunA17_Chr7g0214531 77.178 574 130 1 116 688 7 580 0.0 944
MsaG039005 MtrunA17_Chr1g0213321 69.606 635 174 7 64 688 32 657 0.0 913
MsaG039005 MtrunA17_Chr3g0140431 72.444 577 150 2 116 688 8 579 0.0 895
MsaG039005 MtrunA17_Chr1g0146301 69.637 606 169 4 89 688 69 665 0.0 887
MsaG039005 MtrunA17_Chr8g0340491 69.497 577 170 5 116 688 98 672 0.0 822
MsaG039005 MtrunA17_Chr4g0071031 37.716 289 162 6 139 424 4 277 4.62e-55 195
MsaG039005 MtrunA17_Chr2g0329981 37.024 289 164 6 139 424 4 277 6.83e-54 192
MsaG039005 MtrunA17_Chr8g0342821 37.370 289 162 7 139 424 4 276 5.30e-53 190
MsaG039005 MtrunA17_Chr2g0299661 35.986 289 167 6 139 424 4 277 1.22e-52 189
MsaG039005 MtrunA17_Chr1g0166821 36.525 282 163 5 146 424 10 278 4.06e-52 187
MsaG039005 MtrunA17_Chr7g0261501 35.816 282 165 5 146 424 9 277 1.62e-51 185
MsaG039005 MtrunA17_Chr1g0167261 36.972 284 161 6 146 424 10 280 7.60e-51 180
MsaG039005 MtrunA17_Chr1g0187571 36.170 282 164 5 146 424 10 278 3.15e-50 182
MsaG039005 MtrunA17_Chr8g0390211 36.860 293 164 9 136 424 2 277 3.54e-49 178
MsaG039005 MtrunA17_Chr1g0183631 35.461 282 166 5 146 424 9 277 4.48e-49 178
MsaG039005 MtrunA17_Chr6g0486611 35.689 283 164 6 146 424 9 277 4.87e-49 177
MsaG039005 MtrunA17_Chr3g0140071 34.982 283 166 6 146 424 9 277 7.30e-47 172
MsaG039005 MtrunA17_Chr1g0146151 34.276 283 168 6 146 424 9 277 1.59e-45 167
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039005 AT3G03940.1 81.304 690 119 6 1 688 1 682 0.0 1137
MsaG039005 AT5G18190.2 79.420 690 122 7 1 688 1 672 0.0 1092
MsaG039005 AT5G18190.1 79.420 690 122 7 1 688 1 672 0.0 1092
MsaG039005 AT3G13670.1 73.024 582 152 2 112 688 103 684 0.0 917
MsaG039005 AT2G25760.2 70.984 579 158 5 119 688 80 657 0.0 885
MsaG039005 AT2G25760.1 70.660 576 162 3 119 688 80 654 0.0 884
MsaG039005 AT3G03930.1 74.009 227 58 1 463 688 43 269 1.81e-117 353
MsaG039005 AT1G03930.1 37.234 282 161 5 146 424 9 277 3.82e-54 193
MsaG039005 AT4G26100.1 37.024 289 164 6 139 424 4 277 5.27e-54 192
MsaG039005 AT3G23340.1 37.943 282 159 5 146 424 9 277 1.23e-53 191
MsaG039005 AT5G44100.1 37.234 282 161 5 146 424 9 277 2.69e-53 191
MsaG039005 AT5G44100.2 37.234 282 161 5 146 424 9 277 2.69e-53 191
MsaG039005 AT1G04440.1 37.589 282 160 5 146 424 9 277 4.22e-53 190
MsaG039005 AT5G57015.1 37.024 289 164 6 139 424 4 277 4.40e-53 189
MsaG039005 AT2G19470.1 37.024 289 164 6 139 424 4 277 1.99e-52 187
MsaG039005 AT5G43320.1 36.525 282 163 5 146 424 9 277 4.90e-52 187
MsaG039005 AT4G14340.1 36.879 282 162 5 146 424 15 283 8.97e-52 186
MsaG039005 AT1G72710.1 37.024 289 163 7 139 424 4 276 1.03e-51 186
MsaG039005 AT4G14340.2 36.879 282 162 5 146 424 15 283 1.65e-51 183
MsaG039005 AT4G28540.1 36.525 282 163 5 146 424 13 281 2.36e-51 186
MsaG039005 AT5G43320.2 36.525 282 163 5 146 424 9 277 9.11e-51 181
MsaG039005 AT5G43320.3 36.525 282 163 5 146 424 9 277 9.11e-51 181
MsaG039005 AT4G28880.3 37.809 283 158 6 146 424 9 277 1.28e-50 182
MsaG039005 AT4G28880.2 37.809 283 158 6 146 424 9 277 1.28e-50 182
MsaG039005 AT4G28880.1 37.809 283 158 6 146 424 9 277 1.28e-50 182
MsaG039005 AT4G28880.4 37.809 283 158 6 146 424 9 277 1.28e-50 182
MsaG039005 AT4G28860.2 37.102 283 160 6 146 424 9 277 1.30e-50 181
MsaG039005 AT4G28860.1 37.102 283 160 6 146 424 9 277 1.62e-50 182
MsaG039005 AT3G23340.2 37.736 265 154 5 166 424 4 263 4.12e-48 175
MsaG039005 AT4G08800.1 31.724 290 148 7 138 424 3 245 2.66e-36 138

Find 224 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCATCGCAATGTTATTAT+TGG 0.162623 7:-83623760 None:intergenic
TAGAATTGATGGAGCTTTAA+AGG 0.179699 7:+83625921 MsaT039005.1:CDS
AACAGTAAAGGTTGTAATTA+TGG 0.186107 7:+83624455 MsaT039005.1:CDS
ACATTGATATTTCTCATAAA+AGG 0.197104 7:+83625581 MsaT039005.1:CDS
GATATAAGCTTGGAATAATT+TGG 0.209647 7:-83625853 None:intergenic
ACAAGGAGATTTCTACATTT+TGG 0.215030 7:+83624674 MsaT039005.1:CDS
GCTTCTAGGAACAGCTTAAA+TGG 0.224554 7:-83625802 None:intergenic
TTCCAGAACCTGTTGCATTA+AGG 0.230638 7:+83623703 MsaT039005.1:CDS
CGGAGCAAGATTGATTGATT+TGG 0.246716 7:+83623659 MsaT039005.1:CDS
TATCTTATTGATCTTGGTTT+AGG 0.259935 7:+83625189 MsaT039005.1:CDS
TCAATAGCTGGTGCAGTTAA+TGG 0.273974 7:+83626810 MsaT039005.1:CDS
ACGACCTCAGGATTGGATTA+TGG 0.275540 7:+83626758 MsaT039005.1:intron
TCATGTTAGTAACTGCTTCT+AGG 0.275600 7:-83625816 None:intergenic
CCTATGAGTGGCAAGTTTAC+AGG 0.276750 7:+83624483 MsaT039005.1:CDS
GAGGGTGGAAGTGGTGATAA+AGG 0.280467 7:+83623780 MsaT039005.1:CDS
TGTTGTCTTCAGCTTCTAAA+TGG 0.280494 7:+83625341 MsaT039005.1:intron
ATCAATTACCTCTGCTTCAT+TGG 0.285869 7:-83626159 None:intergenic
TAATTACAGGATTACTTCTA+TGG 0.288626 7:+83627966 MsaT039005.1:CDS
GACTACTGCTGGCAGTCGTT+GGG 0.288669 7:+83627751 MsaT039005.1:CDS
TTGAGACCTATTAGAATTGA+TGG 0.289935 7:+83625910 MsaT039005.1:CDS
GGCGGTAATGCCACAGGTTT+AGG 0.312451 7:+83623582 MsaT039005.1:CDS
ATCAGAATACCCAGCATTCC+TGG 0.312595 7:-83627786 None:intergenic
GCCCTTAATGCAACAGGTTC+TGG 0.315068 7:-83623705 None:intergenic
GCTAGTGTACATGCACATTT+AGG 0.315855 7:+83625515 MsaT039005.1:CDS
TTCCTTTCTGTCTTATATAC+AGG 0.318561 7:-83624196 None:intergenic
TCCAGAACCTGTTGCATTAA+GGG 0.320427 7:+83623704 MsaT039005.1:CDS
CATACTTGGGCCCAGCCTTT+GGG 0.328803 7:+83624782 MsaT039005.1:CDS
TTGTAGGACTGCTGTGTATA+AGG 0.332041 7:-83627659 None:intergenic
ACATACTTGGGCCCAGCCTT+TGG 0.344051 7:+83624781 MsaT039005.1:CDS
AAAGCTCCATCAATTCTAAT+AGG 0.345290 7:-83625916 None:intergenic
TTCTTATCATCAGCCGTTCC+AGG 0.346048 7:-83625162 None:intergenic
CAATTCTCTCCTTGATCAAC+AGG 0.347510 7:-83623504 None:intergenic
TCATCAAGTAATTTGCGTTT+TGG 0.347553 7:-83628027 None:intergenic
ATCAGAGACCTGATATATTC+AGG 0.347579 7:+83625398 MsaT039005.1:CDS
GGCCCAGCCTTTGGGATGTA+TGG 0.350118 7:+83624790 MsaT039005.1:CDS
ATTGCCGTGGCAGGGTTATC+AGG 0.351940 7:+83625607 MsaT039005.1:CDS
AATGGCTGTTATGGCATTCC+CGG 0.358781 7:+83624633 MsaT039005.1:CDS
AAATGATTCGCTCACTTTGT+AGG 0.366607 7:-83627675 None:intergenic
AAGTTGGGTAAGGGCGGTTT+CGG 0.367540 7:+83624217 MsaT039005.1:CDS
CTGTTCCTGCATCCATGATC+AGG 0.368840 7:-83626597 None:intergenic
AGGAAGGATGCTTATTAATC+TGG 0.370481 7:+83626056 MsaT039005.1:CDS
CGACAGGATGTCACCAAGTA+TGG 0.371024 7:+83624931 MsaT039005.1:intron
GAAGATGGGAAAATAATTAC+AGG 0.381298 7:+83627953 MsaT039005.1:CDS
AAGTTATATCTTATTGATCT+TGG 0.385212 7:+83625183 MsaT039005.1:CDS
AGGAGAGTTGGAGGTGGAAG+AGG 0.385580 7:+83623555 MsaT039005.1:CDS
TCCTGCGCCCTTAATGCAAC+AGG 0.395038 7:-83623711 None:intergenic
AAAGGATTTACCCTTTCAAA+TGG 0.397776 7:-83624997 None:intergenic
GCGAATCATTTCCTTTCAAA+TGG 0.401857 7:+83627687 MsaT039005.1:CDS
TGACTACTGCTGGCAGTCGT+TGG 0.405397 7:+83627750 MsaT039005.1:CDS
ACAGTAAAGGTTGTAATTAT+GGG 0.406747 7:+83624456 MsaT039005.1:CDS
TATGTTGGCAGAAGGGTTAG+TGG 0.411408 7:+83624247 MsaT039005.1:CDS
TTTCAGGTTATGGACATACT+TGG 0.411506 7:+83624768 MsaT039005.1:intron
AGCAATCTGAATGGCTGTTA+TGG 0.414069 7:+83624624 MsaT039005.1:intron
CTTCTAGGAACAGCTTAAAT+GGG 0.416955 7:-83625801 None:intergenic
CATTCCCGGGGTTCATTACA+AGG 0.417787 7:+83624647 MsaT039005.1:CDS
GTTGGAGGTGGAAGAGGAAG+AGG 0.417969 7:+83623561 MsaT039005.1:CDS
AGGGTGGAAGTGGTGATAAA+GGG 0.418036 7:+83623781 MsaT039005.1:CDS
ACTACCTCGTTATTGTAAAC+AGG 0.420447 7:-83623735 None:intergenic
GTGATTCAAGGTTGTGGAGC+TGG 0.423726 7:+83627900 MsaT039005.1:intron
TGGGATGTATGGAATTCTCT+TGG 0.430414 7:+83624801 MsaT039005.1:CDS
ACTATAACGTAGCAGACAAT+AGG 0.433982 7:+83626497 MsaT039005.1:CDS
CATTGATATTTCTCATAAAA+GGG 0.435498 7:+83625582 MsaT039005.1:CDS
GGGTGGAAGTGGTGATAAAG+GGG 0.437883 7:+83623782 MsaT039005.1:CDS
CTGTTGATCAAGGAGAGAAT+TGG 0.443896 7:+83623505 MsaT039005.1:CDS
TTTACAAACCTGAATATATC+AGG 0.445016 7:-83625406 None:intergenic
AAATGGAAGGATGCATCATC+TGG 0.447277 7:+83625358 MsaT039005.1:CDS
AACAGAACTAGGAGGAGAGT+TGG 0.448810 7:+83623543 MsaT039005.1:CDS
ATGAGACTCAAGAGACTTTG+CGG 0.449087 7:+83628046 MsaT039005.1:CDS
CTGATCATGGATGCAGGAAC+AGG 0.449323 7:+83626598 MsaT039005.1:CDS
TATAAGACAGAAAGGAAGTT+GGG 0.453128 7:+83624202 MsaT039005.1:CDS
CCGGTGACAACTCATAAACT+TGG 0.455516 7:-83626630 None:intergenic
TCTCCTTGATCAACAGGTTG+TGG 0.455647 7:-83623498 None:intergenic
GTAGTTATGTCCAGGAATGC+TGG 0.457000 7:+83627776 MsaT039005.1:CDS
GAAGATACCCTCTCAGGGAC+AGG 0.457209 7:-83623834 None:intergenic
TGGCCTCTTCAGCGAATTTG+TGG 0.461128 7:+83626572 MsaT039005.1:CDS
ATGCCACAGGTTTAGGTAAA+GGG 0.462923 7:+83623589 MsaT039005.1:CDS
CCTGTAAACTTGCCACTCAT+AGG 0.466576 7:-83624483 None:intergenic
TGTTGATCAAGGAGAGAATT+GGG 0.467095 7:+83623506 MsaT039005.1:CDS
AAAAGGGAGATTGCCGTGGC+AGG 0.468289 7:+83625598 MsaT039005.1:CDS
TGATCAACAGGTTGTGGACC+AGG 0.469318 7:-83623492 None:intergenic
TGCAGTTAATGGCAGTTCCT+TGG 0.471533 7:+83626821 MsaT039005.1:CDS
GTGAGGGAATCCATCGAAGA+TGG 0.472999 7:+83627938 MsaT039005.1:CDS
AATAAAGAAGATACCCTCTC+AGG 0.473552 7:-83623840 None:intergenic
ACTGAGGACTCTTATCCTCT+TGG 0.473776 7:-83628188 None:intergenic
GAATGCTGGGTATTCTGATC+AGG 0.474555 7:+83627790 MsaT039005.1:CDS
GGCCTCTTCAGCGAATTTGT+GGG 0.476058 7:+83626573 MsaT039005.1:CDS
TATGGCTGCTACTAATGATC+AGG 0.477831 7:+83627984 MsaT039005.1:CDS
GGATTGGATTATGGAGCAAT+GGG 0.478370 7:+83626767 MsaT039005.1:intron
GTTCGTCTCGTGGGTACAGC+TGG 0.482477 7:-83623618 None:intergenic
TCCTTGGTTGTCATGTCCAA+AGG 0.482708 7:+83626837 MsaT039005.1:CDS
ATGGCTGTTATGGCATTCCC+GGG 0.484904 7:+83624634 MsaT039005.1:CDS
ACTGATATCCACTGAGTAGC+AGG 0.485351 7:-83626120 None:intergenic
CTTGAGAAGCTCCATTTGAA+AGG 0.486534 7:+83624986 MsaT039005.1:CDS
CTTGCTCCACTTCTCAACTC+TGG 0.487643 7:-83623441 None:intergenic
ATTCCATACATCCCAAAGGC+TGG 0.487823 7:-83624793 None:intergenic
TTGAGAAGCTCCATTTGAAA+GGG 0.491630 7:+83624987 MsaT039005.1:CDS
TTTGCTTGGTCAACCTGGAA+CGG 0.496523 7:+83625149 MsaT039005.1:CDS
ATAAACAAGAAGTGGAAAGA+AGG 0.496800 7:+83627710 MsaT039005.1:CDS
TATACCTTTACATGGGTGCT+AGG 0.497641 7:-83628081 None:intergenic
TGCCCTTGTCTACATGCTGC+CGG 0.497684 7:-83626522 None:intergenic
GCAATGCAAGCAGCCATACT+TGG 0.499840 7:-83624944 None:intergenic
CTCCTGTATATAAGACAGAA+AGG 0.500560 7:+83624194 MsaT039005.1:CDS
CTCCTAGTTCTGTTCTGCGC+AGG 0.501750 7:-83623534 None:intergenic
TACTATATCAGTTCAATAGC+TGG 0.503115 7:+83626798 MsaT039005.1:CDS
AATGCCACAGGTTTAGGTAA+AGG 0.503786 7:+83623588 MsaT039005.1:CDS
CTTCGATGGATTCCCTCACT+TGG 0.504489 7:-83627934 None:intergenic
CAATAATAACATTGCGATGG+AGG 0.504988 7:+83623761 MsaT039005.1:CDS
AGACGAACTGCAGCTGGTAG+AGG 0.505471 7:+83623633 MsaT039005.1:CDS
AGAACTAGGAGGAGAGTTGG+AGG 0.506418 7:+83623546 MsaT039005.1:CDS
TCAATTCTAATAGGTCTCAA+TGG 0.509245 7:-83625907 None:intergenic
CCAACCTTGTGCAGGAAGGC+CGG 0.510823 7:-83626649 None:intergenic
AATGCAAATATACCTTTACA+TGG 0.513801 7:-83628089 None:intergenic
AAGGGATGATCTTGAGTCAC+TGG 0.514728 7:+83625553 MsaT039005.1:CDS
GACAGAAAGGAAGTTGGGTA+AGG 0.515309 7:+83624207 MsaT039005.1:CDS
AGTAATACCTCAATAGCATC+AGG 0.516051 7:-83624289 None:intergenic
GTCAAGTTTATGTTGGCAGA+AGG 0.517543 7:+83624239 MsaT039005.1:CDS
TTCTTGTTTATCCATTTGAA+AGG 0.517956 7:-83627698 None:intergenic
TTCAGGTTATGGACATACTT+GGG 0.519606 7:+83624769 MsaT039005.1:intron
GTCTTCAGCTTCTAAATGGA+AGG 0.520168 7:+83625345 MsaT039005.1:intron
GAATTTGTGGGCCCTGATCA+TGG 0.521370 7:+83626585 MsaT039005.1:CDS
AAAGAAAGCCAACCTTGTGC+AGG 0.526157 7:-83626657 None:intergenic
GGAAGGAGGTCACAAGGTTG+AGG 0.530053 7:-83623684 None:intergenic
ACTGCAGCTGGTAGAGGCCG+CGG 0.531027 7:+83623639 MsaT039005.1:CDS
ACCTGTTGCATTAAGGGCGC+AGG 0.531495 7:+83623710 MsaT039005.1:CDS
GCAAGAAGGTCAAAGCGGCT+TGG 0.531798 7:+83623459 MsaT039005.1:CDS
CTGTAAACTTGCCACTCATA+GGG 0.533466 7:-83624482 None:intergenic
ACCAGCTGCAGTTCGTCTCG+TGG 0.534661 7:-83623628 None:intergenic
CAGTAGTCATGGAGGTAACA+TGG 0.536716 7:-83627736 None:intergenic
GGTCCACAACCTGTTGATCA+AGG 0.537351 7:+83623495 MsaT039005.1:CDS
ATTACCTCTGCTTCATTGGA+CGG 0.538904 7:-83626155 None:intergenic
AGGAACAGCTTAAATGGGGC+GGG 0.539923 7:-83625796 None:intergenic
AGGATTGGATTATGGAGCAA+TGG 0.542568 7:+83626766 MsaT039005.1:intron
GGCTGCTTGCATTGCAGTGG+AGG 0.545351 7:+83624952 MsaT039005.1:CDS
CCCACGAGACGAACTGCAGC+TGG 0.546076 7:+83623627 MsaT039005.1:CDS
ATACCTTTACATGGGTGCTA+GGG 0.546088 7:-83628080 None:intergenic
AGTAGGTCCTGATGCTATTG+AGG 0.546466 7:+83624282 MsaT039005.1:CDS
ATATAAGACAGAAAGGAAGT+TGG 0.546790 7:+83624201 MsaT039005.1:CDS
TCAAATGGATAAACAAGAAG+TGG 0.550338 7:+83627702 MsaT039005.1:CDS
AATAACATTGCGATGGAGGG+TGG 0.551319 7:+83623765 MsaT039005.1:CDS
GTAGACAAGGGCATTGAGGA+TGG 0.553137 7:+83626532 MsaT039005.1:CDS
CGACCCTTGTAATGAACCCC+GGG 0.553304 7:-83624651 None:intergenic
GGTTTCGGTCAAGTTTATGT+TGG 0.554643 7:+83624232 MsaT039005.1:CDS
GGCCCACAAATTCGCTGAAG+AGG 0.554852 7:-83626575 None:intergenic
TTTCCCTAGCACCCATGTAA+AGG 0.556145 7:+83628077 MsaT039005.1:CDS
TTTGAGCATAGAAACAGTAA+AGG 0.556925 7:+83624443 MsaT039005.1:CDS
GTTGGGGCGTAGTTATGTCC+AGG 0.558365 7:+83627768 MsaT039005.1:CDS
TTCCATACATCCCAAAGGCT+GGG 0.558942 7:-83624792 None:intergenic
CCAAGTTTATGAGTTGTCAC+CGG 0.561482 7:+83626630 MsaT039005.1:CDS
GTCACCGGCCTTCCTGCACA+AGG 0.561560 7:+83626645 MsaT039005.1:CDS
TCAAGTTTATGTTGGCAGAA+GGG 0.561965 7:+83624240 MsaT039005.1:CDS
GTTACCTCCATGACTACTGC+TGG 0.562071 7:+83627740 MsaT039005.1:CDS
AAGATACCCTCTCAGGGACA+GGG 0.562274 7:-83623833 None:intergenic
CGGCTTGGAGATCTTCAGCC+TGG 0.563701 7:+83623474 MsaT039005.1:CDS
TGAGGGAATCCATCGAAGAT+GGG 0.567161 7:+83627939 MsaT039005.1:CDS
TAGGTAGGACTGGCAGTCGA+AGG 0.568222 7:+83625534 MsaT039005.1:CDS
CATGCACATTTAGGTAGGAC+TGG 0.571892 7:+83625524 MsaT039005.1:CDS
GGAAGAGGCGGTAATGCCAC+AGG 0.572031 7:+83623576 MsaT039005.1:CDS
ACGACTGCCAGCAGTAGTCA+TGG 0.572203 7:-83627747 None:intergenic
GGAGGTGGAAGAGGAAGAGG+CGG 0.573185 7:+83623564 MsaT039005.1:CDS
TGGGCCCTGATCATGGATGC+AGG 0.575619 7:+83626592 MsaT039005.1:CDS
TAAACAAGAAGTGGAAAGAA+GGG 0.576099 7:+83627711 MsaT039005.1:CDS
ACTAGGAGGAGAGTTGGAGG+TGG 0.576453 7:+83623549 MsaT039005.1:CDS
AGCCTGCGCAGAACAGAACT+AGG 0.577364 7:+83623532 MsaT039005.1:CDS
AATAATAACATTGCGATGGA+GGG 0.577523 7:+83623762 MsaT039005.1:CDS
ATAAAGAAGATACCCTCTCA+GGG 0.577785 7:-83623839 None:intergenic
ATAGGTCTCAATGGAGTGCA+TGG 0.578825 7:-83625898 None:intergenic
ATTGCGATGGAGGGTGGAAG+TGG 0.579631 7:+83623771 MsaT039005.1:CDS
AGGATGCCAGAGTTGAGAAG+TGG 0.581316 7:+83623435 None:intergenic
CCAGCTGCAGTTCGTCTCGT+GGG 0.582642 7:-83623627 None:intergenic
GCATGTAGACAAGGGCATTG+AGG 0.583537 7:+83626528 MsaT039005.1:CDS
TAGTTATGTCCAGGAATGCT+GGG 0.584964 7:+83627777 MsaT039005.1:CDS
AAAGGGAGATTGCCGTGGCA+GGG 0.586233 7:+83625599 MsaT039005.1:CDS
ACGTAGCAGACAATAGGCTC+CGG 0.587905 7:+83626503 MsaT039005.1:CDS
GGCCAATAATAACATTGCGA+TGG 0.587946 7:+83623758 MsaT039005.1:CDS
ACTACTGCTGGCAGTCGTTG+GGG 0.590324 7:+83627752 MsaT039005.1:CDS
TGTTTACAATAACGAGGTAG+TGG 0.593077 7:+83623737 MsaT039005.1:CDS
CATTGAATCAGGTTTGTGCA+CGG 0.597171 7:+83625105 MsaT039005.1:intron
TAGACAAGGGCATTGAGGAT+GGG 0.597926 7:+83626533 MsaT039005.1:CDS
ACAGAAAGGAAGTTGGGTAA+GGG 0.598318 7:+83624208 MsaT039005.1:CDS
AACACATCAACTCCATGAAG+TGG 0.599242 7:-83625765 None:intergenic
TGTTCCTGCATCCATGATCA+GGG 0.601851 7:-83626596 None:intergenic
CACAACCCCTGTCCCTGAGA+GGG 0.602420 7:+83623827 MsaT039005.1:CDS
TACTAACATGAGATTTGATG+AGG 0.603551 7:+83625828 MsaT039005.1:CDS
TCGACCCTTGTAATGAACCC+CGG 0.603691 7:-83624652 None:intergenic
ATGCAACAGGTTCTGGAAGG+AGG 0.603984 7:-83623698 None:intergenic
AGTTGAGAAGTGGAGCAAGA+AGG 0.609115 7:+83623445 MsaT039005.1:CDS
CGCACCTGATAACCCTGCCA+CGG 0.610039 7:-83625611 None:intergenic
AGGTAGGACTGGCAGTCGAA+GGG 0.615002 7:+83625535 MsaT039005.1:CDS
GTATTTATGCAGGTGCAAGT+TGG 0.615502 7:+83624166 MsaT039005.1:intron
GTTGGCAGAAGGGTTAGTGG+TGG 0.615848 7:+83624250 MsaT039005.1:CDS
AGTGGTGGCTCTGATCGAGT+AGG 0.615874 7:+83624265 MsaT039005.1:CDS
CGGTGACAACTCATAAACTT+GGG 0.618245 7:-83626629 None:intergenic
TAGGAACAGCTTAAATGGGG+CGG 0.618479 7:-83625797 None:intergenic
GTTCATTACAAGGGTCGACA+AGG 0.618757 7:+83624657 MsaT039005.1:CDS
GCTCCGGCAGCATGTAGACA+AGG 0.619103 7:+83626519 MsaT039005.1:CDS
GTGTACATGCACATTTAGGT+AGG 0.623393 7:+83625519 MsaT039005.1:CDS
GCATTGATAAGCAATCTGAA+TGG 0.623419 7:+83624615 MsaT039005.1:intron
AAAGCCAACCTTGTGCAGGA+AGG 0.628987 7:-83626653 None:intergenic
TTAATGCAACAGGTTCTGGA+AGG 0.631616 7:-83623701 None:intergenic
GGTTCTGGAAGGAGGTCACA+AGG 0.637818 7:-83623690 None:intergenic
TGGAAGTGGTGATAAAGGGG+TGG 0.639830 7:+83623785 MsaT039005.1:CDS
CACGCCGTCCAATGAAGCAG+AGG 0.644205 7:+83626151 MsaT039005.1:CDS
TCATAAAAGGGAGATTGCCG+TGG 0.646563 7:+83625594 MsaT039005.1:CDS
AGATACCCTCTCAGGGACAG+GGG 0.647323 7:-83623832 None:intergenic
GAAAGGAAGTTGGGTAAGGG+CGG 0.647435 7:+83624211 MsaT039005.1:CDS
TATGGCTGCTTGCATTGCAG+TGG 0.656459 7:+83624949 MsaT039005.1:CDS
ATTCCCGGGGTTCATTACAA+GGG 0.656914 7:+83624648 MsaT039005.1:CDS
ATGCAAATATACCTTTACAT+GGG 0.657780 7:-83628088 None:intergenic
TGGGTAGTCCTGCTACTCAG+TGG 0.660145 7:+83626112 MsaT039005.1:CDS
ATTATGGGCCTCCCTATGAG+TGG 0.662879 7:+83624471 MsaT039005.1:CDS
GCACAACCCCTGTCCCTGAG+AGG 0.664978 7:+83623826 MsaT039005.1:CDS
ACCTTTGGACATGACAACCA+AGG 0.669025 7:-83626838 None:intergenic
TAAACTTGCCACTCATAGGG+AGG 0.673865 7:-83624479 None:intergenic
CTGCGCAGAACAGAACTAGG+AGG 0.676245 7:+83623535 MsaT039005.1:CDS
TTCTAGGAACAGCTTAAATG+GGG 0.677821 7:-83625800 None:intergenic
CTCCGGCAGCATGTAGACAA+GGG 0.681418 7:+83626520 MsaT039005.1:CDS
GAGAATTCCATACATCCCAA+AGG 0.684665 7:-83624797 None:intergenic
TGGCTGTTATGGCATTCCCG+GGG 0.691580 7:+83624635 MsaT039005.1:CDS
GTGGAGCAAGAAGGTCAAAG+CGG 0.702956 7:+83623454 MsaT039005.1:CDS
AGACCCTTTACCTAAACCTG+TGG 0.726840 7:-83623592 None:intergenic
AATCAATCAATCTTGCTCCG+CGG 0.728884 7:-83623656 None:intergenic
GGAACCTGTTTACAATAACG+AGG 0.748265 7:+83623731 MsaT039005.1:CDS
ACTGCCAGCAGTAGTCATGG+AGG 0.768146 7:-83627744 None:intergenic
TGGGTTGTATATAAGCTGTG+TGG 0.777779 7:+83626552 MsaT039005.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 83623438 83628199 83623438 ID=MsaG039005
Chr7 mRNA 83623438 83628199 83623438 ID=MsaT039005.1;Parent=MsaG039005
Chr7 exon 83623438 83623848 83623438 ID=MsaT039005.1.exon1;Parent=MsaT039005.1
Chr7 CDS 83623438 83623848 83623438 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83624178 83624303 83624178 ID=MsaT039005.1.exon2;Parent=MsaT039005.1
Chr7 CDS 83624178 83624303 83624178 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83624431 83624504 83624431 ID=MsaT039005.1.exon3;Parent=MsaT039005.1
Chr7 CDS 83624431 83624504 83624431 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83624626 83624695 83624626 ID=MsaT039005.1.exon4;Parent=MsaT039005.1
Chr7 CDS 83624626 83624695 83624626 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83624774 83624829 83624774 ID=MsaT039005.1.exon5;Parent=MsaT039005.1
Chr7 CDS 83624774 83624829 83624774 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83624937 83625008 83624937 ID=MsaT039005.1.exon6;Parent=MsaT039005.1
Chr7 CDS 83624937 83625008 83624937 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83625116 83625210 83625116 ID=MsaT039005.1.exon7;Parent=MsaT039005.1
Chr7 CDS 83625116 83625210 83625116 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83625353 83625419 83625353 ID=MsaT039005.1.exon8;Parent=MsaT039005.1
Chr7 CDS 83625353 83625419 83625353 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83625499 83625630 83625499 ID=MsaT039005.1.exon9;Parent=MsaT039005.1
Chr7 CDS 83625499 83625630 83625499 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83625726 83625942 83625726 ID=MsaT039005.1.exon10;Parent=MsaT039005.1
Chr7 CDS 83625726 83625942 83625726 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83626042 83626172 83626042 ID=MsaT039005.1.exon11;Parent=MsaT039005.1
Chr7 CDS 83626042 83626172 83626042 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83626492 83626666 83626492 ID=MsaT039005.1.exon12;Parent=MsaT039005.1
Chr7 CDS 83626492 83626666 83626492 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83626768 83626858 83626768 ID=MsaT039005.1.exon13;Parent=MsaT039005.1
Chr7 CDS 83626768 83626858 83626768 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83627654 83627811 83627654 ID=MsaT039005.1.exon14;Parent=MsaT039005.1
Chr7 CDS 83627654 83627811 83627654 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83627910 83628098 83627910 ID=MsaT039005.1.exon15;Parent=MsaT039005.1
Chr7 CDS 83627910 83628098 83627910 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Chr7 exon 83628179 83628199 83628179 ID=MsaT039005.1.exon16;Parent=MsaT039005.1
Chr7 CDS 83628179 83628199 83628179 ID=cds.MsaT039005.1;Parent=MsaT039005.1
Gene Sequence

>MsaT039005.1

ATGCCAGAGTTGAGAAGTGGAGCAAGAAGGTCAAAGCGGCTTGGAGATCTTCAGCCTGGTCCACAACCTGTTGATCAAGGAGAGAATTGGGCAGAGCCTGCGCAGAACAGAACTAGGAGGAGAGTTGGAGGTGGAAGAGGAAGAGGCGGTAATGCCACAGGTTTAGGTAAAGGGTCTTCACCAGCTGTACCCACGAGACGAACTGCAGCTGGTAGAGGCCGCGGAGCAAGATTGATTGATTTGGATCCTCAACCTTGTGACCTCCTTCCAGAACCTGTTGCATTAAGGGCGCAGGAACCTGTTTACAATAACGAGGTAGTGGCCAATAATAACATTGCGATGGAGGGTGGAAGTGGTGATAAAGGGGTGGCAGCTGAAGAAGACGCAAGCACAACCCCTGTCCCTGAGAGGGTGCAAGTTGGCAACTCTCCTGTATATAAGACAGAAAGGAAGTTGGGTAAGGGCGGTTTCGGTCAAGTTTATGTTGGCAGAAGGGTTAGTGGTGGCTCTGATCGAGTAGGTCCTGATGCTATTGAGGTTGCACTCAAGTTTGAGCATAGAAACAGTAAAGGTTGTAATTATGGGCCTCCCTATGAGTGGCAAGTTTACAGCAATCTGAATGGCTGTTATGGCATTCCCGGGGTTCATTACAAGGGTCGACAAGGAGATTTCTACATTTTGGTTATGGACATACTTGGGCCCAGCCTTTGGGATGTATGGAATTCTCTTGGTCAATCGATGTCACCAAGTATGGCTGCTTGCATTGCAGTGGAGGCAATATCAATTCTTGAGAAGCTCCATTTGAAAGGGTTTGTGCACGGAGATGTAAAACCAGAAAACTTTTTGCTTGGTCAACCTGGAACGGCTGATGATAAGAAGTTATATCTTATTGATCTTGGTTTAGCTTCTAAATGGAAGGATGCATCATCTGGTCAACACGTTGAATATGATCAGAGACCTGATATATTCAGGGGAACGATAAGATATGCTAGTGTACATGCACATTTAGGTAGGACTGGCAGTCGAAGGGATGATCTTGAGTCACTGGCATACACATTGATATTTCTCATAAAAGGGAGATTGCCGTGGCAGGGTTATCAGGTGGTGACAATAAAAGTTTTCTTGTTTGTAAGAAAAAATGTCCACTTCATGGAGTTGATGTGTTGCTTTTGTCCCGCCCCATTTAAGCTGTTCCTAGAAGCAGTTACTAACATGAGATTTGATGAGGAGCCAAATTATTCCAAGCTTATATCACTTTTTGACAGCTTGATCGAACCATGCACTCCATTGAGACCTATTAGAATTGATGGAGCTTTAAAGGTTGGTCAAAAACGAGGAAGGATGCTTATTAATCTGGAAGAAGATGAGCAGCCCAAGAAAAAAGTGCGATTGGGTAGTCCTGCTACTCAGTGGATATCAGTGTATAATGCACGCCGTCCAATGAAGCAGAGATATCACTATAACGTAGCAGACAATAGGCTCCGGCAGCATGTAGACAAGGGCATTGAGGATGGGTTGTATATAAGCTGTGTGGCCTCTTCAGCGAATTTGTGGGCCCTGATCATGGATGCAGGAACAGGTTTTTCTTCCCAAGTTTATGAGTTGTCACCGGCCTTCCTGCACAAGGATTGGATTATGGAGCAATGGGAGAAAAATTACTATATCAGTTCAATAGCTGGTGCAGTTAATGGCAGTTCCTTGGTTGTCATGTCCAAAGGAACTCCTTATACACAGCAGTCCTACAAAGTGAGCGAATCATTTCCTTTCAAATGGATAAACAAGAAGTGGAAAGAAGGGTTCCATGTTACCTCCATGACTACTGCTGGCAGTCGTTGGGGCGTAGTTATGTCCAGGAATGCTGGGTATTCTGATCAGGTTGTGGAGCTGGACTTTTTGTATCCAAGTGAGGGAATCCATCGAAGATGGGAAAATAATTACAGGATTACTTCTATGGCTGCTACTAATGATCAGGCAGCCTTCATACTTAGCATACCAAAACGCAAATTACTTGATGAGACTCAAGAGACTTTGCGGACATCTGCTTTCCCTAGCACCCATGTAAAGTTTTTACAGCCAAGAGGATAA
Protein sequence

>MsaT039005.1

MPELRSGARRSKRLGDLQPGPQPVDQGENWAEPAQNRTRRRVGGGRGRGGNATGLGKGSSPAVPTRRTAAGRGRGARLIDLDPQPCDLLPEPVALRAQEPVYNNEVVANNNIAMEGGSGDKGVAAEEDASTTPVPERVQVGNSPVYKTERKLGKGGFGQVYVGRRVSGGSDRVGPDAIEVALKFEHRNSKGCNYGPPYEWQVYSNLNGCYGIPGVHYKGRQGDFYILVMDILGPSLWDVWNSLGQSMSPSMAACIAVEAISILEKLHLKGFVHGDVKPENFLLGQPGTADDKKLYLIDLGLASKWKDASSGQHVEYDQRPDIFRGTIRYASVHAHLGRTGSRRDDLESLAYTLIFLIKGRLPWQGYQVVTIKVFLFVRKNVHFMELMCCFCPAPFKLFLEAVTNMRFDEEPNYSKLISLFDSLIEPCTPLRPIRIDGALKVGQKRGRMLINLEEDEQPKKKVRLGSPATQWISVYNARRPMKQRYHYNVADNRLRQHVDKGIEDGLYISCVASSANLWALIMDAGTGFSSQVYELSPAFLHKDWIMEQWEKNYYISSIAGAVNGSSLVVMSKGTPYTQQSYKVSESFPFKWINKKWKEGFHVTSMTTAGSRWGVVMSRNAGYSDQVVELDFLYPSEGIHRRWENNYRITSMAATNDQAAFILSIPKRKLLDETQETLRTSAFPSTHVKFLQPRG*