AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG039158

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039158 MtrunA17_Chr7g0255181 98.298 705 12 0 1 705 1 705 0.0 1442
MsaG039158 MtrunA17_Chr3g0080761 46.986 730 294 15 1 704 1 663 0.0 642
MsaG039158 MtrunA17_Chr8g0354821 46.027 730 308 17 13 704 6 687 0.0 617
MsaG039158 MtrunA17_Chr5g0399801 40.307 521 281 15 202 704 580 1088 2.55e-117 377
MsaG039158 MtrunA17_Chr4g0003501 40.619 517 278 15 202 702 449 952 1.01e-114 368
MsaG039158 MtrunA17_Chr1g0163051 41.317 501 259 13 203 680 158 646 1.12e-113 357
MsaG039158 MtrunA17_Chr8g0369301 43.220 472 226 17 239 681 280 738 9.09e-109 347
MsaG039158 MtrunA17_Chr8g0368901 41.113 467 243 12 238 680 299 757 1.39e-107 344
MsaG039158 MtrunA17_Chr4g0003511 38.921 519 286 14 202 702 544 1049 1.77e-107 350
MsaG039158 MtrunA17_Chr8g0368911 40.881 477 244 14 231 680 285 750 5.04e-106 340
MsaG039158 MtrunA17_Chr1g0163041 40.343 466 251 13 236 680 106 565 8.44e-102 323
MsaG039158 MtrunA17_Chr6g0467531 38.462 481 253 10 227 680 100 564 4.85e-100 318
MsaG039158 MtrunA17_Chr7g0252211 38.976 508 272 11 228 701 250 753 7.97e-100 323
MsaG039158 MtrunA17_Chr6g0467381 38.462 481 253 10 227 680 100 564 1.08e-99 317
MsaG039158 MtrunA17_Chr6g0467421 38.254 481 254 10 227 680 100 564 2.81e-99 316
MsaG039158 MtrunA17_Chr6g0476111 38.723 501 270 10 234 701 194 690 6.87e-98 317
MsaG039158 MtrunA17_Chr6g0467401 38.046 481 255 10 227 680 100 564 1.41e-97 312
MsaG039158 MtrunA17_Chr6g0476121 38.523 501 271 10 234 701 143 639 9.16e-97 312
MsaG039158 MtrunA17_Chr3g0094881 36.473 499 286 11 218 696 152 639 1.02e-85 283
MsaG039158 MtrunA17_Chr2g0306961 36.000 475 239 12 219 680 41 463 1.14e-80 264
MsaG039158 MtrunA17_Chr6g0467411 33.128 486 267 11 219 680 31 482 4.14e-75 250
MsaG039158 MtrunA17_Chr1g0170651 33.015 524 300 14 203 702 47 543 1.89e-64 224
MsaG039158 MtrunA17_Chr7g0232811 30.228 483 228 17 255 700 124 534 2.80e-55 198
MsaG039158 MtrunA17_Chr7g0232981 30.116 518 263 18 200 700 76 511 1.24e-50 184
MsaG039158 MtrunA17_Chr7g0232841 29.259 540 248 21 200 700 79 523 1.91e-50 184
MsaG039158 MtrunA17_Chr1g0190311 28.916 332 156 12 434 701 325 640 8.68e-33 134
MsaG039158 MtrunA17_Chr5g0403241 32.588 313 166 11 426 702 1201 1504 1.20e-31 132
MsaG039158 MtrunA17_Chr8g0373821 31.699 306 164 14 426 700 242 533 3.04e-30 125
MsaG039158 MtrunA17_Chr1g0183321 30.435 322 161 8 436 702 430 743 8.06e-30 125
MsaG039158 MtrunA17_Chr4g0075321 36.453 203 114 4 505 702 869 1061 2.29e-29 125
MsaG039158 MtrunA17_Chr7g0262001 30.864 324 153 10 436 701 388 698 4.32e-29 123
MsaG039158 MtrunA17_Chr1g0201621 32.065 184 103 5 522 704 61 223 2.33e-19 92.0
MsaG039158 MtrunA17_Chr1g0201311 32.065 184 103 5 522 704 61 223 3.65e-19 91.3
MsaG039158 MtrunA17_Chr3g0125251 32.402 179 101 4 526 704 1306 1464 2.92e-18 90.1
MsaG039158 MtrunA17_Chr6g0460921 29.536 237 144 7 467 702 203 417 6.05e-18 87.0
MsaG039158 MtrunA17_Chr6g0477001 29.348 184 106 5 519 701 87 247 8.44e-17 82.4
MsaG039158 MtrunA17_Chr2g0305371 32.967 182 98 7 523 704 314 471 1.21e-16 83.6
MsaG039158 MtrunA17_Chr5g0448331 31.677 161 94 3 263 414 267 420 9.23e-15 78.2
MsaG039158 MtrunA17_Chr8g0389931 31.481 162 93 4 263 414 261 414 2.21e-14 77.0
MsaG039158 MtrunA17_Chr3g0143561 30.435 230 126 9 263 480 187 394 2.68e-14 75.5
MsaG039158 MtrunA17_Chr7g0232871 50.000 56 26 1 542 597 21 74 1.75e-12 64.3
MsaG039158 MtrunA17_Chr1g0149611 33.121 157 87 5 546 701 365 504 1.98e-12 70.1
MsaG039158 MtrunA17_Chr2g0297191 30.909 165 82 5 543 702 1031 1168 7.79e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039158 AT1G73100.1 47.893 712 291 13 13 704 17 668 0.0 625
MsaG039158 AT5G04940.2 45.588 748 284 14 1 704 1 669 0.0 597
MsaG039158 AT5G04940.1 45.588 748 284 14 1 704 1 669 0.0 597
MsaG039158 AT1G17770.1 44.112 535 249 14 201 704 177 692 3.93e-139 424
MsaG039158 AT2G24740.1 43.667 529 239 16 201 704 260 754 3.08e-124 387
MsaG039158 AT4G13460.1 42.147 503 257 14 203 680 145 638 1.89e-118 369
MsaG039158 AT4G13460.2 42.147 503 257 14 203 680 145 638 1.89e-118 369
MsaG039158 AT2G35160.5 40.504 516 266 15 200 702 268 755 2.57e-115 364
MsaG039158 AT2G35160.2 40.504 516 266 15 200 702 304 791 4.41e-115 365
MsaG039158 AT2G35160.3 40.504 516 266 15 200 702 304 791 4.41e-115 365
MsaG039158 AT2G35160.1 40.504 516 266 15 200 702 304 791 4.41e-115 365
MsaG039158 AT2G35160.4 40.504 516 266 15 200 702 304 791 4.41e-115 365
MsaG039158 AT2G33290.1 41.600 500 261 15 203 680 149 639 4.90e-109 345
MsaG039158 AT2G33290.3 41.600 500 261 15 203 680 149 639 4.90e-109 345
MsaG039158 AT2G33290.4 41.600 500 261 15 203 680 149 639 4.90e-109 345
MsaG039158 AT2G33290.2 41.600 500 261 15 203 680 149 639 4.90e-109 345
MsaG039158 AT2G22740.2 38.374 529 284 16 203 701 270 786 5.62e-103 333
MsaG039158 AT2G22740.1 38.374 529 284 16 203 701 270 786 5.62e-103 333
MsaG039158 AT5G13960.1 37.275 499 276 11 236 701 126 620 1.55e-93 303
MsaG039158 AT5G13960.2 37.500 480 263 11 255 701 11 486 6.11e-92 295
MsaG039158 AT2G05900.1 45.128 195 103 3 276 467 1 194 4.56e-46 166
MsaG039158 AT2G05900.1 57.500 80 30 1 625 704 236 311 8.09e-21 94.4
MsaG039158 AT5G47150.1 40.094 212 117 5 203 409 118 324 8.58e-41 152
MsaG039158 AT3G04380.2 29.819 332 152 11 434 701 117 431 4.63e-33 133
MsaG039158 AT3G04380.3 29.819 332 152 11 434 701 128 442 5.13e-33 133
MsaG039158 AT3G04380.1 29.819 332 152 11 434 701 144 458 5.34e-33 134
MsaG039158 AT3G04380.6 29.819 332 152 11 434 701 117 431 6.77e-33 133
MsaG039158 AT3G04380.5 29.819 332 152 11 434 701 101 415 7.05e-33 132
MsaG039158 AT3G04380.4 29.819 332 152 11 434 701 144 458 7.12e-33 133
MsaG039158 AT2G23740.3 31.475 305 169 12 430 704 1087 1381 7.43e-32 133
MsaG039158 AT2G23740.6 31.475 305 169 12 430 704 1087 1381 7.43e-32 133
MsaG039158 AT2G23740.2 31.475 305 169 12 430 704 1087 1381 7.43e-32 133
MsaG039158 AT2G23740.1 31.475 305 169 12 430 704 1080 1374 8.76e-32 133
MsaG039158 AT5G47160.1 40.976 205 104 7 208 406 215 408 4.67e-31 126
MsaG039158 AT5G47160.3 40.976 205 104 7 208 406 215 408 4.67e-31 126
MsaG039158 AT5G47160.2 40.976 205 104 7 208 406 215 408 4.67e-31 126
MsaG039158 AT1G04050.2 28.834 326 163 10 434 702 407 720 3.02e-27 118
MsaG039158 AT1G04050.1 28.834 326 163 10 434 702 407 720 3.02e-27 118
MsaG039158 AT1G04050.3 28.834 326 163 10 434 702 303 616 3.47e-27 117
MsaG039158 AT5G43990.9 27.900 319 166 10 436 701 418 725 1.77e-23 106
MsaG039158 AT5G43990.2 27.900 319 166 10 436 701 418 725 1.77e-23 106
MsaG039158 AT5G43990.15 27.900 319 166 10 436 701 398 705 1.88e-23 106
MsaG039158 AT5G43990.10 27.900 319 166 10 436 701 398 705 1.88e-23 106
MsaG039158 AT5G43990.16 27.900 319 166 10 436 701 375 682 1.98e-23 106
MsaG039158 AT5G43990.5 27.900 319 166 10 436 701 375 682 1.98e-23 106
MsaG039158 AT5G43990.17 27.900 319 166 10 436 701 375 682 1.98e-23 106
MsaG039158 AT5G43990.14 27.900 319 166 10 436 701 375 682 1.98e-23 106
MsaG039158 AT5G43990.7 27.900 319 166 10 436 701 306 613 2.09e-23 105
MsaG039158 AT5G43990.3 27.900 319 166 10 436 701 395 702 2.28e-23 105
MsaG039158 AT5G43990.11 27.900 319 166 10 436 701 395 702 2.28e-23 105
MsaG039158 AT5G43990.1 27.900 319 166 10 436 701 395 702 2.28e-23 105
MsaG039158 AT5G43990.4 27.900 319 166 10 436 701 395 702 2.28e-23 105
MsaG039158 AT5G43990.8 27.900 319 166 10 436 701 326 633 2.41e-23 105
MsaG039158 AT5G43990.12 27.900 319 166 10 436 701 326 633 2.41e-23 105
MsaG039158 AT5G43990.6 27.900 319 166 10 436 701 326 633 2.41e-23 105
MsaG039158 AT5G43990.13 27.900 319 166 10 436 701 326 633 2.41e-23 105
MsaG039158 AT3G03750.2 33.023 215 125 6 495 702 145 347 5.49e-23 101
MsaG039158 AT2G23740.4 30.802 237 126 10 430 637 1087 1314 3.39e-20 96.7
MsaG039158 AT2G23740.5 30.802 237 126 10 430 637 1087 1314 3.39e-20 96.7
MsaG039158 AT1G76710.2 31.016 187 101 5 522 704 66 228 3.32e-18 89.0
MsaG039158 AT1G76710.1 31.016 187 101 5 522 704 66 228 3.32e-18 89.0
MsaG039158 AT1G76710.4 31.016 187 101 5 522 704 66 228 4.48e-18 88.6
MsaG039158 AT1G76710.3 31.016 187 101 5 522 704 66 228 4.76e-18 88.6
MsaG039158 AT1G77300.2 29.609 179 106 4 526 704 1008 1166 2.27e-17 87.4
MsaG039158 AT1G77300.1 29.609 179 106 4 526 704 1008 1166 4.03e-17 86.7
MsaG039158 AT4G30860.1 30.769 182 102 5 523 704 307 464 1.00e-16 84.3
MsaG039158 AT3G03750.1 29.302 215 121 6 495 704 145 333 2.68e-16 81.3
MsaG039158 AT1G57820.1 32.927 164 89 6 263 414 277 431 1.34e-14 77.8
MsaG039158 AT1G57820.3 32.927 164 89 6 263 414 277 431 1.34e-14 77.8
MsaG039158 AT3G59960.3 30.573 157 87 4 545 701 112 246 4.35e-14 73.9
MsaG039158 AT3G59960.2 30.573 157 87 4 545 701 112 246 4.35e-14 73.9
MsaG039158 AT3G59960.4 30.573 157 87 4 545 701 112 246 4.35e-14 73.9
MsaG039158 AT3G59960.1 30.573 157 87 4 545 701 112 246 1.54e-13 72.8
MsaG039158 AT2G44150.1 30.573 157 87 4 545 701 117 251 1.57e-13 73.2
MsaG039158 AT5G42400.7 31.293 147 79 5 556 702 1261 1385 1.41e-11 68.9
MsaG039158 AT5G42400.2 31.293 147 79 5 556 702 1261 1385 1.41e-11 68.9
MsaG039158 AT5G42400.8 31.293 147 79 5 556 702 1261 1385 1.41e-11 68.9
MsaG039158 AT5G42400.5 31.293 147 79 5 556 702 1261 1385 1.41e-11 68.9
MsaG039158 AT5G42400.4 31.293 147 79 5 556 702 1261 1385 1.41e-11 68.9
MsaG039158 AT5G42400.1 31.293 147 79 5 556 702 1261 1385 1.41e-11 68.9
MsaG039158 AT5G42400.3 31.293 147 79 5 556 702 1261 1385 1.41e-11 68.9
MsaG039158 AT5G42400.6 31.293 147 79 5 556 702 1261 1385 1.41e-11 68.9
MsaG039158 AT2G31650.1 30.769 169 96 5 534 702 888 1035 8.76e-11 66.2

Find 229 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTCAATGGATCATAATT+TGG 0.115607 7:-85361417 None:intergenic
ATGGAGTTAACTTATGATTA+TGG 0.170067 7:-85359397 MsaT039158.1:CDS
GGGATTACATTCACCTTCTA+TGG 0.257350 7:-85360574 MsaT039158.1:CDS
CTCTTCCTCCTGACTTGCTT+TGG 0.269905 7:+85360530 None:intergenic
GGAGTCTTGAATGAGTTAAT+TGG 0.284019 7:+85361122 None:intergenic
TTCCATATTGTATATGCTTC+AGG 0.299380 7:+85360183 None:intergenic
GAATAGCATCCCACGATCTT+AGG 0.312805 7:+85359740 None:intergenic
TGAGACAAAGAAAGGGTAAA+AGG 0.313393 7:+85361223 None:intergenic
AATGGAGATGTTGGCTCGTC+AGG 0.322654 7:-85361086 MsaT039158.1:CDS
TCATTTGGTTGTTCTTGTAT+TGG 0.334935 7:-85359976 MsaT039158.1:CDS
GGCAAAATAACCCCTGTTCT+TGG 0.336157 7:+85360129 None:intergenic
TAAAGCGGGGATGGGATCTT+TGG 0.343049 7:+85359568 None:intergenic
CAATGATGTGAGAGTCATTA+GGG 0.348715 7:-85360343 MsaT039158.1:CDS
CAATTTGTCCAAGCTTTCTT+CGG 0.349022 7:+85360763 None:intergenic
AAACCATGGGGTTAATTGAT+TGG 0.354540 7:+85360856 None:intergenic
GCTCCTGTGGAATCTTCATT+TGG 0.355899 7:-85359991 MsaT039158.1:CDS
TTTGTTGCTGTTTCTCCTGC+TGG 0.355971 7:-85361269 MsaT039158.1:CDS
CCTGACTTGCTTTGGAAGTC+AGG 0.358642 7:+85360538 None:intergenic
GCCATTGAGGACTCTTGTTC+CGG 0.364278 7:-85361348 MsaT039158.1:CDS
TGGAGTTAACTTATGATTAT+GGG 0.364704 7:-85359396 MsaT039158.1:CDS
AGCTGGCCTAAAGTTTCGTT+TGG 0.364801 7:-85359794 MsaT039158.1:CDS
TGTTCTTGGAGCAGACTTAT+CGG 0.365671 7:+85360143 None:intergenic
TTTACAAGATTCAAGATTCA+TGG 0.365754 7:-85360269 MsaT039158.1:CDS
TCAACAAAGGACCTTCATAC+TGG 0.371795 7:-85360735 MsaT039158.1:CDS
CCTGCTGGTCCTTTCCCTGC+TGG 0.373673 7:-85361254 MsaT039158.1:CDS
CAGCTTGGGAAATCCGATTT+CGG 0.374618 7:+85359812 None:intergenic
TGCTCAATGGATCATAATTT+GGG 0.378337 7:-85361416 None:intergenic
GCAATGATGTGAGAGTCATT+AGG 0.383731 7:-85360344 MsaT039158.1:CDS
TGAAAGGGGTAATCTTGCTT+TGG 0.386578 7:-85360385 MsaT039158.1:CDS
ATCGGACGCCACAACACATT+TGG 0.391864 7:+85359490 None:intergenic
ACATCGTTAACAAGGCAAAC+AGG 0.397211 7:+85360078 None:intergenic
ATTGGAACTGCAGCAGATAT+TGG 0.404786 7:+85361140 None:intergenic
AAGGATGTAATGCATCCATC+TGG 0.405471 7:-85360315 MsaT039158.1:CDS
TAGAACTAGTAACAAAGGTT+GGG 0.411501 7:-85359764 MsaT039158.1:CDS
GATAAGTCTGCTCCAAGAAC+AGG 0.411674 7:-85360141 MsaT039158.1:CDS
GCTGTTAGTATTGTCTCATC+TGG 0.412567 7:-85360501 MsaT039158.1:CDS
GCCCTCCTACCTGCCGAAAT+CGG 0.414777 7:-85359825 MsaT039158.1:CDS
ATTAACCCGGCTGCAGAATA+CGG 0.416172 7:+85359895 None:intergenic
GCAATGTTATGATGACTAAA+GGG 0.424137 7:-85360686 MsaT039158.1:CDS
ACATTGCCTGCCTTCAAATC+AGG 0.424451 7:+85360702 None:intergenic
CCTGACTTCCAAAGCAAGTC+AGG 0.426734 7:-85360538 MsaT039158.1:CDS
CTTATCGGTCCATTGTTGAA+TGG 0.429349 7:+85360158 None:intergenic
GAGAGTCATTAGGGGTTTGA+AGG 0.431227 7:-85360334 MsaT039158.1:CDS
TCATAAGTTAACTCCATCAT+AGG 0.442222 7:+85359403 None:intergenic
TGGCCTAAAGTTTCGTTTGG+AGG 0.442228 7:-85359791 MsaT039158.1:CDS
TAATCAATTCCAGCCATAGA+AGG 0.443168 7:+85360561 None:intergenic
CCGATTTGAGATGTGCTTAG+TGG 0.445932 7:-85360595 MsaT039158.1:CDS
ACGAAACTTTAGGCCAGCTT+GGG 0.447136 7:+85359798 None:intergenic
ACTCCAACTGCGGCGACAAA+TGG 0.448731 7:-85361104 MsaT039158.1:CDS
ATCGTGCCTGGTGTTGAAAT+TGG 0.450040 7:-85360633 MsaT039158.1:CDS
TAAAAGGGTGCAACACCAGC+AGG 0.450352 7:+85361239 None:intergenic
AACGAAACTTTAGGCCAGCT+TGG 0.452928 7:+85359797 None:intergenic
GAGACAATACTAACAGCTAA+TGG 0.456036 7:+85360507 None:intergenic
GCAGGAGAAACAGCAACAAA+AGG 0.456434 7:+85361272 None:intergenic
AGCCTGAAGCATATACAATA+TGG 0.461673 7:-85360185 MsaT039158.1:CDS
TGGGTGGAGAAAGCAAAATC+TGG 0.461943 7:-85360249 MsaT039158.1:CDS
GCTAGTGGTGCTGCTACCGA+TGG 0.462293 7:-85360933 MsaT039158.1:CDS
TAAATCATCCTGATGGTAGT+AGG 0.464869 7:-85360824 MsaT039158.1:CDS
TGTCCTTGCATTCAAAAGAA+TGG 0.465660 7:-85359928 MsaT039158.1:CDS
TTATGATGACTAAAGGGGTT+AGG 0.468940 7:-85360680 MsaT039158.1:CDS
TCCCCACTCTCAAGAATTTG+AGG 0.470672 7:-85360020 MsaT039158.1:CDS
TTTGGAGCTTCAATGTTAGC+AGG 0.470974 7:+85359586 None:intergenic
TTACATTCACCTTCTATGGC+TGG 0.473841 7:-85360570 MsaT039158.1:CDS
GGCAATGTTATGATGACTAA+AGG 0.475883 7:-85360687 MsaT039158.1:CDS
GGCAAAGAGACCTGATTTGA+AGG 0.476296 7:-85360712 MsaT039158.1:CDS
GTGTGGCACGTTGAGGAGTT+GGG 0.476415 7:+85361162 None:intergenic
TGTATTGGTGGGTGTCAACC+TGG 0.477276 7:-85359961 MsaT039158.1:CDS
CAAGTGATCAAAAGCTTGAA+AGG 0.477698 7:-85360401 MsaT039158.1:CDS
TATATCACAGCAAAGAATGA+AGG 0.479624 7:-85359547 MsaT039158.1:CDS
TATCTTCCGTATTCTGCAGC+CGG 0.481263 7:-85359901 MsaT039158.1:CDS
GTCTCTTTGCCCCAGTATGA+AGG 0.482308 7:+85360724 None:intergenic
TTACCCTTTCTTTGTCTCAC+CGG 0.483307 7:-85361219 MsaT039158.1:CDS
CAAGGACAGTTATGATTGCC+AGG 0.485014 7:+85359943 None:intergenic
TAGACACATCCCTCCTATGA+TGG 0.488114 7:-85359416 MsaT039158.1:CDS
GGTGTGGCACGTTGAGGAGT+TGG 0.489443 7:+85361161 None:intergenic
CAACAAAGGACCTTCATACT+GGG 0.490499 7:-85360734 MsaT039158.1:CDS
GCACGTTGAGGAGTTGGGTT+CGG 0.491402 7:+85361167 None:intergenic
CCCCTCAAATTCTTGAGAGT+GGG 0.492249 7:+85360018 None:intergenic
CTGTACCTGCTGATAAGTCC+AGG 0.493660 7:-85361385 MsaT039158.1:CDS
TTCAGCCCCAGAAGTAAGAT+CGG 0.498712 7:+85360107 None:intergenic
TCTCCATTTGTCGCCGCAGT+TGG 0.498811 7:+85361101 None:intergenic
AGGTGGAGTTAACCGTGAAA+AGG 0.500138 7:-85360427 MsaT039158.1:CDS
GAAGAGGGCAAAGGGACGAC+GGG 0.500240 7:-85360955 MsaT039158.1:CDS
GGAGATGTGTTGATTTATAG+TGG 0.500452 7:-85360453 MsaT039158.1:CDS
CCACTAAGCACATCTCAAAT+CGG 0.500926 7:+85360595 None:intergenic
TTAGAACTAGTAACAAAGGT+TGG 0.502849 7:-85359765 MsaT039158.1:CDS
TCAGAAAGCCTCTGAGACTC+CGG 0.504695 7:+85361200 None:intergenic
ACGTGAAAATAAGAATGAAC+CGG 0.506046 7:-85359464 MsaT039158.1:CDS
TTACAAGATTCAAGATTCAT+GGG 0.506441 7:-85360268 MsaT039158.1:CDS
AAGAAAGCAATGTGGAGATC+CGG 0.506521 7:+85359445 None:intergenic
GGGATGTAAGTGAAATATGC+AGG 0.508325 7:+85360039 None:intergenic
TTGGTTGTTCTTGTATTGGT+GGG 0.508807 7:-85359972 MsaT039158.1:CDS
GCTCGTCAGGGAGGAAAAGC+CGG 0.508854 7:-85361073 MsaT039158.1:CDS
ATTGATGTAGATGCTGAAAC+TGG 0.512685 7:-85360999 MsaT039158.1:CDS
AAATCATCCTGATGGTAGTA+GGG 0.513621 7:-85360823 MsaT039158.1:CDS
AAGTGATCAAAAGCTTGAAA+GGG 0.514412 7:-85360400 MsaT039158.1:CDS
ACACCTCCAAACGAAACTTT+AGG 0.515803 7:+85359788 None:intergenic
ATCCGATTTCGGCAGGTAGG+AGG 0.516883 7:+85359823 None:intergenic
GAAGTGCTCCGAAGAAAGCT+TGG 0.518002 7:-85360771 MsaT039158.1:CDS
AAGAGACCTGATTTGAAGGC+AGG 0.518276 7:-85360708 MsaT039158.1:CDS
CGTTATCAACATCGTTAACA+AGG 0.518388 7:+85360070 None:intergenic
ATGAAGGGTTAGACGGCGAC+GGG 0.519190 7:+85361321 None:intergenic
CGATTTGAGATGTGCTTAGT+GGG 0.519994 7:-85360594 MsaT039158.1:CDS
TCAGCCCCAGAAGTAAGATC+GGG 0.521108 7:+85360108 None:intergenic
GGAGGATATGAAGATGATAC+AGG 0.521134 7:-85360480 MsaT039158.1:CDS
TTGGGAAATCCGATTTCGGC+AGG 0.521804 7:+85359816 None:intergenic
GGGAAGGTATATGTCTATGA+TGG 0.523697 7:-85360294 MsaT039158.1:CDS
CCCCACTCTCAAGAATTTGA+GGG 0.524461 7:-85360019 MsaT039158.1:CDS
TATAAGTTAGCGAGGGTGCG+TGG 0.525394 7:-85360210 MsaT039158.1:CDS
GGTGGAGTTAACCGTGAAAA+GGG 0.526500 7:-85360426 MsaT039158.1:CDS
TCTTTGCTGTGATATAAAGC+GGG 0.528039 7:+85359554 None:intergenic
ATTATCGAAGCCTTCTTCCT+CGG 0.530806 7:+85361031 None:intergenic
CGAAATCGGATTTCCCAAGC+TGG 0.531644 7:-85359811 MsaT039158.1:CDS
GGACAGCTTGCCGAGGAAGA+AGG 0.532376 7:-85361041 MsaT039158.1:CDS
AGTAACAGCAAGAAAAGGAT+AGG 0.534489 7:-85360657 MsaT039158.1:CDS
TCGTGGGATGCTATTCGTGC+TGG 0.534850 7:-85359733 MsaT039158.1:CDS
AATGCTAGAGCCGAGATGCT+TGG 0.536191 7:-85359673 MsaT039158.1:CDS
TGGCTTAACATTTAAAACCC+TGG 0.537287 7:+85361367 None:intergenic
ATCTTCCGTATTCTGCAGCC+GGG 0.537741 7:-85359900 MsaT039158.1:CDS
TCCCCTCAAATTCTTGAGAG+TGG 0.538709 7:+85360017 None:intergenic
TTCTGCAGCCGGGTTAATTG+CGG 0.542999 7:-85359890 MsaT039158.1:CDS
CTGTGATATAAAGCGGGGAT+GGG 0.543576 7:+85359560 None:intergenic
AAAACCCTGGACTTATCAGC+AGG 0.546637 7:+85361380 None:intergenic
GCGGCGACAAATGGAGATGT+TGG 0.546685 7:-85361095 MsaT039158.1:CDS
AGAACTAGTAACAAAGGTTG+GGG 0.547727 7:-85359763 MsaT039158.1:CDS
AAGCGCAAGAAGAGGGCAAA+GGG 0.547955 7:-85360963 MsaT039158.1:CDS
TTTGGTTGTTCTTGTATTGG+TGG 0.548158 7:-85359973 MsaT039158.1:CDS
TTCTTTGCTGTGATATAAAG+CGG 0.548348 7:+85359553 None:intergenic
TAAGTTAACTCCATCATAGG+AGG 0.550333 7:+85359406 None:intergenic
TCCGATTTCGGCAGGTAGGA+GGG 0.551928 7:+85359824 None:intergenic
ATAAACCTTCCTCTAAAAGC+AGG 0.552575 7:-85359373 MsaT039158.1:CDS
GCAGGTAGGAGGGCACTGAC+AGG 0.553761 7:+85359834 None:intergenic
TTCCTCTAAAAGCAGGTCAA+AGG 0.554167 7:-85359366 MsaT039158.1:CDS
TTTGAGGGGAGCTGCTCCTG+TGG 0.557376 7:-85360004 MsaT039158.1:CDS
AGAAGAGGGCAAAGGGACGA+CGG 0.558358 7:-85360956 MsaT039158.1:CDS
TGTGGATTTGAAGATGATGA+AGG 0.558678 7:+85361305 None:intergenic
TCTCCAATCAATTAACCCCA+TGG 0.559218 7:-85360859 MsaT039158.1:CDS
GAAGATGATGAAGGGTTAGA+CGG 0.565606 7:+85361314 None:intergenic
CATCGTTAACAAGGCAAACA+GGG 0.565923 7:+85360079 None:intergenic
CCAGCAGGGAAAGGACCAGC+AGG 0.566022 7:+85361254 None:intergenic
GAGACAAAGAAAGGGTAAAA+GGG 0.567219 7:+85361224 None:intergenic
GACTCCGGTGAGACAAAGAA+AGG 0.567937 7:+85361215 None:intergenic
GCAAAGGGACGACGGGCTAG+TGG 0.568187 7:-85360948 MsaT039158.1:CDS
CTAGTGGTGCTGCTACCGAT+GGG 0.568231 7:-85360932 MsaT039158.1:CDS
ACGGAGTCCCTACTACCATC+AGG 0.568604 7:+85360816 None:intergenic
CCCTCAAATTCTTGAGAGTG+GGG 0.569490 7:+85360019 None:intergenic
TATTTAAGTATAAGTTAGCG+AGG 0.570308 7:-85360218 MsaT039158.1:CDS
ATGGAGATGTTGGCTCGTCA+GGG 0.572634 7:-85361085 MsaT039158.1:CDS
TTAGGAGTAACAGCAAGAAA+AGG 0.573552 7:-85360662 MsaT039158.1:CDS
GATGTAATAAATCATCCTGA+TGG 0.577400 7:-85360831 MsaT039158.1:CDS
GCTGTGATATAAAGCGGGGA+TGG 0.577821 7:+85359559 None:intergenic
GATGAAGGGTTAGACGGCGA+CGG 0.579808 7:+85361320 None:intergenic
ATAAGTCTGCTCCAAGAACA+GGG 0.580680 7:-85360140 MsaT039158.1:CDS
AAGGTTGGGGCCTAAGATCG+TGG 0.581600 7:-85359750 MsaT039158.1:CDS
ATATCACAGCAAAGAATGAA+GGG 0.583808 7:-85359546 MsaT039158.1:CDS
AAAAGGATAGGAATCGTGCC+TGG 0.584823 7:-85360645 MsaT039158.1:CDS
TTTGCCCGATCTTACTTCTG+GGG 0.586246 7:-85360112 MsaT039158.1:CDS
GGCAGCCACACCACTCCCAT+CGG 0.586262 7:+85360917 None:intergenic
AGAATGAAGGGAATGTTGCT+CGG 0.587254 7:-85359534 MsaT039158.1:CDS
TAGACATATACCTTCCCAGA+TGG 0.587685 7:+85360300 None:intergenic
GGTGCAACACCAGCAGGGAA+AGG 0.589413 7:+85361245 None:intergenic
TATGAAGATGATACAGGCGA+TGG 0.593001 7:-85360474 MsaT039158.1:CDS
GATATTGGTGTGGCACGTTG+AGG 0.595796 7:+85361155 None:intergenic
TATCATAAGTGTATATGTCA+CGG 0.598394 7:+85360797 None:intergenic
CAAGCTGTCCACGACGACTC+CGG 0.601601 7:+85361054 None:intergenic
AACGATGTTGATAACGAGAA+GGG 0.603649 7:-85360063 MsaT039158.1:CDS
TAACGATGTTGATAACGAGA+AGG 0.606192 7:-85360064 MsaT039158.1:CDS
GGAGTTCAAAGCGCAAGAAG+AGG 0.608143 7:-85360971 MsaT039158.1:CDS
AGGATGTAATGCATCCATCT+GGG 0.610008 7:-85360314 MsaT039158.1:CDS
CTCATTCAAGACTCCAACTG+CGG 0.610227 7:-85361114 MsaT039158.1:CDS
GGTGCTGCTACCGATGGGAG+TGG 0.611341 7:-85360927 MsaT039158.1:CDS
TATGGAAATCCATTCAACAA+TGG 0.612304 7:-85360167 MsaT039158.1:CDS
GAGTTCAAAGCGCAAGAAGA+GGG 0.616539 7:-85360970 MsaT039158.1:CDS
AGGTTGGGGCCTAAGATCGT+GGG 0.616709 7:-85359749 MsaT039158.1:CDS
TTGTCTCACCGGAGTCTCAG+AGG 0.617372 7:-85361208 MsaT039158.1:CDS
GTGTTGATTTATAGTGGCCA+AGG 0.617841 7:-85360447 MsaT039158.1:CDS
ACCGGAACAAGAGTCCTCAA+TGG 0.618399 7:+85361347 None:intergenic
CCTTGCATTCAAAAGAATGG+AGG 0.618509 7:-85359925 MsaT039158.1:CDS
ATAAGTTAGCGAGGGTGCGT+GGG 0.618572 7:-85360209 MsaT039158.1:CDS
AGGAAAAGCCGGAGTCGTCG+TGG 0.619315 7:-85361062 MsaT039158.1:CDS
CAAGATTCAAGATTCATGGG+TGG 0.619850 7:-85360265 MsaT039158.1:CDS
GTTAGTATTGTCTCATCTGG+AGG 0.619982 7:-85360498 MsaT039158.1:CDS
TGCGGATCTGTGAAATGCAG+AGG 0.620494 7:-85359328 MsaT039158.1:CDS
CGCATCTAGATCAACATCAA+CGG 0.622116 7:+85360896 None:intergenic
GCTGCTCTCCAAATGTGTTG+TGG 0.624395 7:-85359498 MsaT039158.1:CDS
GTGTTTAGAACTAGTAACAA+AGG 0.626486 7:-85359769 MsaT039158.1:CDS
TTAGACGGCGACGGGAACAC+CGG 0.627096 7:+85361329 None:intergenic
ACTCCGGTGAGACAAAGAAA+GGG 0.627426 7:+85361216 None:intergenic
CTTTGCTGTGATATAAAGCG+GGG 0.627888 7:+85359555 None:intergenic
GTGGATTTGAAGATGATGAA+GGG 0.627913 7:+85361306 None:intergenic
TGCTACCGATGGGAGTGGTG+TGG 0.628585 7:-85360922 MsaT039158.1:CDS
AAAGCGCAAGAAGAGGGCAA+AGG 0.629220 7:-85360964 MsaT039158.1:CDS
TTTCACGGTTAACTCCACCT+TGG 0.630077 7:+85360430 None:intergenic
TGTAATGCATCCATCTGGGA+AGG 0.632252 7:-85360310 MsaT039158.1:CDS
GTAGATGCTGAAACTGGTGG+TGG 0.632727 7:-85360993 MsaT039158.1:CDS
TCGTCGTGGACAGCTTGCCG+AGG 0.633483 7:-85361048 MsaT039158.1:CDS
CAACCAAATGAAGATTCCAC+AGG 0.635037 7:+85359988 None:intergenic
GTAGGAGGGCACTGACAGGA+AGG 0.639143 7:+85359838 None:intergenic
ATATCTCCAATTTCAACACC+AGG 0.640900 7:+85360627 None:intergenic
AAGTTAACTCCATCATAGGA+GGG 0.642935 7:+85359407 None:intergenic
GCTAGAGCCGAGATGCTTGG+TGG 0.644480 7:-85359670 MsaT039158.1:CDS
GAAATCCGATTTCGGCAGGT+AGG 0.644525 7:+85359820 None:intergenic
CAATGTTATGATGACTAAAG+GGG 0.647984 7:-85360685 MsaT039158.1:CDS
TGTACCTGCTGATAAGTCCA+GGG 0.650337 7:-85361384 MsaT039158.1:CDS
AGGACCACACTCATGTATCA+CGG 0.651954 7:+85359858 None:intergenic
TTGATTTATAGTGGCCAAGG+TGG 0.653607 7:-85360444 MsaT039158.1:CDS
CCCACTCTCAAGAATTTGAG+GGG 0.657095 7:-85360018 MsaT039158.1:CDS
ACAAAAGGAGCACCACCTTG+TGG 0.660948 7:+85361287 None:intergenic
AGTGATCAAAAGCTTGAAAG+GGG 0.661761 7:-85360399 MsaT039158.1:CDS
TGATGTTGATCTAGATGCGG+TGG 0.666046 7:-85360892 MsaT039158.1:CDS
ATTTAAGTATAAGTTAGCGA+GGG 0.667098 7:-85360217 MsaT039158.1:CDS
GACTTCCAAAGCAAGTCAGG+AGG 0.671552 7:-85360535 MsaT039158.1:CDS
GGATGTAAGTGAAATATGCA+GGG 0.671677 7:+85360040 None:intergenic
AAAAGGGTGCAACACCAGCA+GGG 0.675547 7:+85361240 None:intergenic
ACAAATTGAAGAGTCAACAA+AGG 0.676116 7:-85360748 MsaT039158.1:CDS
TTGGAAAAGAGTATGCATAG+AGG 0.686529 7:-85360366 MsaT039158.1:CDS
TAGATGCTGAAACTGGTGGT+GGG 0.691190 7:-85360992 MsaT039158.1:CDS
CGTTGATGTTGATCTAGATG+CGG 0.698170 7:-85360895 MsaT039158.1:CDS
AGAAGGCTTCGATAATACTG+AGG 0.700744 7:-85361024 MsaT039158.1:CDS
GAGATGTTGGCTCGTCAGGG+AGG 0.706223 7:-85361082 MsaT039158.1:CDS
AACAAAGGACCTTCATACTG+GGG 0.708757 7:-85360733 MsaT039158.1:CDS
AAATCCGTGATACATGAGTG+TGG 0.714442 7:-85359862 MsaT039158.1:CDS
TCATCATCTTCAAATCCACA+AGG 0.718031 7:-85361302 MsaT039158.1:CDS
TCATCTTCAAATCCACAAGG+TGG 0.722787 7:-85361299 MsaT039158.1:CDS
TAAGTCTGCTCCAAGAACAG+GGG 0.730768 7:-85360139 MsaT039158.1:CDS
GATGTAGATGCTGAAACTGG+TGG 0.738350 7:-85360996 MsaT039158.1:CDS
AACTGCAGCAGATATTGGTG+TGG 0.741595 7:+85361145 None:intergenic
AATGATGTGAGAGTCATTAG+GGG 0.755750 7:-85360342 MsaT039158.1:CDS
TAATTGCAAAGAAAGCAATG+TGG 0.756556 7:+85359437 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 85359315 85361432 85359315 ID=MsaG039158
Chr7 mRNA 85359315 85361432 85359315 ID=MsaT039158.1;Parent=MsaG039158
Chr7 exon 85359315 85361432 85359315 ID=MsaT039158.1.exon1;Parent=MsaT039158.1
Chr7 CDS 85359315 85361432 85359315 ID=cds.MsaT039158.1;Parent=MsaT039158.1
Gene Sequence

>MsaT039158.1

ATGGATCATAATTTGGGTCAAGAATCTGTACCTGCTGATAAGTCCAGGGTTTTAAATGTTAAGCCATTGAGGACTCTTGTTCCGGTGTTCCCGTCGCCGTCTAACCCTTCATCATCTTCAAATCCACAAGGTGGTGCTCCTTTTGTTGCTGTTTCTCCTGCTGGTCCTTTCCCTGCTGGTGTTGCACCCTTTTACCCTTTCTTTGTCTCACCGGAGTCTCAGAGGCTTTCTGAACAACATGCACCGAACCCAACTCCTCAACGTGCCACACCAATATCTGCTGCAGTTCCAATTAACTCATTCAAGACTCCAACTGCGGCGACAAATGGAGATGTTGGCTCGTCAGGGAGGAAAAGCCGGAGTCGTCGTGGACAGCTTGCCGAGGAAGAAGGCTTCGATAATACTGAGGTAATTGATGTAGATGCTGAAACTGGTGGTGGGAGTTCAAAGCGCAAGAAGAGGGCAAAGGGACGACGGGCTAGTGGTGCTGCTACCGATGGGAGTGGTGTGGCTGCCGTTGATGTTGATCTAGATGCGGTGGCTCATGATATTCTCCAATCAATTAACCCCATGGTTTTTGATGTAATAAATCATCCTGATGGTAGTAGGGACTCCGTGACATATACACTTATGATATATGAAGTGCTCCGAAGAAAGCTTGGACAAATTGAAGAGTCAACAAAGGACCTTCATACTGGGGCAAAGAGACCTGATTTGAAGGCAGGCAATGTTATGATGACTAAAGGGGTTAGGAGTAACAGCAAGAAAAGGATAGGAATCGTGCCTGGTGTTGAAATTGGAGATATTTTCTTTTTCCGATTTGAGATGTGCTTAGTGGGATTACATTCACCTTCTATGGCTGGAATTGATTACCTGACTTCCAAAGCAAGTCAGGAGGAAGAGCCATTAGCTGTTAGTATTGTCTCATCTGGAGGATATGAAGATGATACAGGCGATGGAGATGTGTTGATTTATAGTGGCCAAGGTGGAGTTAACCGTGAAAAGGGAGCAAGTGATCAAAAGCTTGAAAGGGGTAATCTTGCTTTGGAAAAGAGTATGCATAGAGGCAATGATGTGAGAGTCATTAGGGGTTTGAAGGATGTAATGCATCCATCTGGGAAGGTATATGTCTATGATGGAATTTACAAGATTCAAGATTCATGGGTGGAGAAAGCAAAATCTGGATTCAATGTATTTAAGTATAAGTTAGCGAGGGTGCGTGGGCAGCCTGAAGCATATACAATATGGAAATCCATTCAACAATGGACCGATAAGTCTGCTCCAAGAACAGGGGTTATTTTGCCCGATCTTACTTCTGGGGCTGAAAAAGTCCCTGTTTGCCTTGTTAACGATGTTGATAACGAGAAGGGCCCTGCATATTTCACTTACATCCCCACTCTCAAGAATTTGAGGGGAGCTGCTCCTGTGGAATCTTCATTTGGTTGTTCTTGTATTGGTGGGTGTCAACCTGGCAATCATAACTGTCCTTGCATTCAAAAGAATGGAGGTTATCTTCCGTATTCTGCAGCCGGGTTAATTGCGGACCTAAAATCCGTGATACATGAGTGTGGTCCTTCCTGTCAGTGCCCTCCTACCTGCCGAAATCGGATTTCCCAAGCTGGCCTAAAGTTTCGTTTGGAGGTGTTTAGAACTAGTAACAAAGGTTGGGGCCTAAGATCGTGGGATGCTATTCGTGCTGGTACTTTTATATGTGAGTATGCAGGGGAAGTCATAGATAATGCTAGAGCCGAGATGCTTGGTGGTGAAAATGAAGACGAATACATTTTTGATTCTACTCGCATCTATCAGCAGCTGCAAGTTTTTCCTGCTAACATTGAAGCTCCAAAGATCCCATCCCCGCTTTATATCACAGCAAAGAATGAAGGGAATGTTGCTCGGTTTATGAATCATAGCTGCTCTCCAAATGTGTTGTGGCGTCCGATTGTACGTGAAAATAAGAATGAACCGGATCTCCACATTGCTTTCTTTGCAATTAGACACATCCCTCCTATGATGGAGTTAACTTATGATTATGGGATAAACCTTCCTCTAAAAGCAGGTCAAAGGAAAAAGAATTGCTTATGCGGATCTGTGAAATGCAGAGGTTACTTTTGTTAA
Protein sequence

>MsaT039158.1

MDHNLGQESVPADKSRVLNVKPLRTLVPVFPSPSNPSSSSNPQGGAPFVAVSPAGPFPAGVAPFYPFFVSPESQRLSEQHAPNPTPQRATPISAAVPINSFKTPTAATNGDVGSSGRKSRSRRGQLAEEEGFDNTEVIDVDAETGGGSSKRKKRAKGRRASGAATDGSGVAAVDVDLDAVAHDILQSINPMVFDVINHPDGSRDSVTYTLMIYEVLRRKLGQIEESTKDLHTGAKRPDLKAGNVMMTKGVRSNSKKRIGIVPGVEIGDIFFFRFEMCLVGLHSPSMAGIDYLTSKASQEEEPLAVSIVSSGGYEDDTGDGDVLIYSGQGGVNREKGASDQKLERGNLALEKSMHRGNDVRVIRGLKDVMHPSGKVYVYDGIYKIQDSWVEKAKSGFNVFKYKLARVRGQPEAYTIWKSIQQWTDKSAPRTGVILPDLTSGAEKVPVCLVNDVDNEKGPAYFTYIPTLKNLRGAAPVESSFGCSCIGGCQPGNHNCPCIQKNGGYLPYSAAGLIADLKSVIHECGPSCQCPPTCRNRISQAGLKFRLEVFRTSNKGWGLRSWDAIRAGTFICEYAGEVIDNARAEMLGGENEDEYIFDSTRIYQQLQVFPANIEAPKIPSPLYITAKNEGNVARFMNHSCSPNVLWRPIVRENKNEPDLHIAFFAIRHIPPMMELTYDYGINLPLKAGQRKKNCLCGSVKCRGYFC*