AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG039204

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039204 MtrunA17_Chr7g0255711 97.030 303 4 3 1 302 1 299 0.0 584
MsaG039204 MtrunA17_Chr4g0031961 44.545 220 63 5 5 182 8 210 5.72e-44 152
MsaG039204 MtrunA17_Chr4g0067981 66.176 68 23 0 86 153 210 277 3.05e-24 102
MsaG039204 MtrunA17_Chr2g0304841 58.442 77 32 0 90 166 212 288 1.02e-22 98.2
MsaG039204 MtrunA17_Chr2g0294911 60.294 68 27 0 86 153 206 273 4.06e-22 96.3
MsaG039204 MtrunA17_Chr3g0137101 54.545 77 34 1 78 154 186 261 5.11e-22 96.3
MsaG039204 MtrunA17_Chr1g0161431 60.938 64 25 0 90 153 199 262 7.44e-21 92.8
MsaG039204 MtrunA17_Chr1g0149841 65.000 60 21 0 90 149 190 249 3.04e-20 90.5
MsaG039204 MtrunA17_Chr4g0074401 65.574 61 21 0 88 148 200 260 4.62e-20 90.5
MsaG039204 MtrunA17_Chr4g0074411 65.574 61 21 0 88 148 198 258 3.84e-19 87.8
MsaG039204 MtrunA17_Chr6g0473731 52.857 70 33 0 88 157 92 161 6.01e-19 86.7
MsaG039204 MtrunA17_Chr1g0149831 63.333 60 19 1 90 149 127 183 7.26e-18 80.9
MsaG039204 MtrunA17_Chr4g0074421 54.688 64 29 0 82 145 124 187 1.10e-17 79.7
MsaG039204 MtrunA17_Chr7g0275891 51.899 79 22 2 90 155 190 265 1.37e-17 82.0
MsaG039204 MtrunA17_Chr8g0376881 56.164 73 28 1 89 157 207 279 1.94e-17 82.4
MsaG039204 MtrunA17_Chr3g0123131 51.389 72 35 0 90 161 198 269 7.23e-17 80.9
MsaG039204 MtrunA17_Chr5g0400241 56.667 60 26 0 90 149 310 369 1.26e-16 80.1
MsaG039204 MtrunA17_Chr4g0054981 55.000 60 27 0 90 149 309 368 5.33e-16 78.2
MsaG039204 MtrunA17_Chr1g0149851 45.349 86 32 1 90 160 190 275 6.21e-16 78.2
MsaG039204 MtrunA17_Chr4g0009851 56.897 58 25 0 90 147 168 225 1.15e-15 75.9
MsaG039204 MtrunA17_Chr4g0040481 51.667 60 29 0 90 149 157 216 1.77e-15 76.3
MsaG039204 MtrunA17_Chr2g0324891 34.351 131 51 3 90 208 160 267 2.45e-15 75.9
MsaG039204 MtrunA17_Chr4g0045361 50.746 67 32 1 84 149 267 333 1.47e-14 73.6
MsaG039204 MtrunA17_Chr4g0009981 55.172 58 26 0 90 147 115 172 2.06e-14 71.6
MsaG039204 MtrunA17_Chr4g0009901 54.237 59 27 0 90 148 167 225 5.04e-14 70.9
MsaG039204 MtrunA17_Chr4g0062921 52.381 63 29 1 87 148 231 293 6.68e-14 71.6
MsaG039204 MtrunA17_Chr7g0275401 45.122 82 41 1 90 171 191 268 8.07e-14 70.5
MsaG039204 MtrunA17_Chr5g0417521 56.364 55 23 1 91 144 15 69 1.39e-13 70.5
MsaG039204 MtrunA17_Chr8g0356501 61.818 55 20 1 91 144 37 91 2.79e-13 69.3
MsaG039204 MtrunA17_Chr2g0321461 55.385 65 27 2 91 154 45 108 2.86e-13 69.7
MsaG039204 MtrunA17_Chr6g0461641 40.000 95 45 3 91 181 42 128 8.70e-13 68.2
MsaG039204 MtrunA17_Chr2g0310011 60.000 55 21 1 91 144 35 89 1.10e-12 67.8
MsaG039204 MtrunA17_Chr1g0197171 46.429 84 41 2 81 160 31 114 1.16e-12 67.0
MsaG039204 MtrunA17_Chr2g0290991 50.685 73 35 1 91 162 39 111 1.19e-12 67.4
MsaG039204 MtrunA17_Chr1g0198601 50.000 60 29 1 90 148 210 269 1.58e-12 67.4
MsaG039204 MtrunA17_Chr7g0261911 50.725 69 31 2 91 158 262 328 1.71e-12 67.4
MsaG039204 MtrunA17_Chr5g0402631 51.667 60 28 1 90 148 213 272 1.72e-12 67.0
MsaG039204 MtrunA17_Chr1g0175081 44.928 69 37 1 77 144 8 76 1.88e-12 62.8
MsaG039204 MtrunA17_Chr5g0422621 51.667 60 28 1 90 148 197 256 2.29e-12 66.6
MsaG039204 MtrunA17_Chr5g0409731 56.364 55 23 1 91 144 25 79 3.46e-12 65.9
MsaG039204 MtrunA17_Chr3g0134481 47.170 53 28 0 90 142 131 183 5.57e-12 63.5
MsaG039204 MtrunA17_Chr1g0190301 55.556 54 23 1 91 143 175 228 6.90e-12 65.5
MsaG039204 MtrunA17_Chr6g0471041 47.458 59 31 0 90 148 171 229 7.37e-12 65.5
MsaG039204 MtrunA17_Chr2g0291021 41.667 96 45 2 60 144 8 103 1.23e-11 64.3
MsaG039204 MtrunA17_Chr4g0042451 52.727 55 25 1 91 144 17 71 1.25e-11 64.3
MsaG039204 MtrunA17_Chr8g0366281 41.905 105 54 4 43 143 125 226 2.37e-11 63.5
MsaG039204 MtrunA17_Chr7g0274471 53.704 54 24 1 91 143 218 271 2.67e-11 63.9
MsaG039204 MtrunA17_Chr1g0162931 56.364 55 23 1 90 143 17 71 6.58e-11 62.0
MsaG039204 MtrunA17_Chr7g0254981 48.529 68 34 1 78 144 37 104 8.05e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039204 AT3G10760.1 56.160 349 92 10 1 302 1 335 6.74e-92 276
MsaG039204 AT5G05090.1 54.870 308 91 11 1 302 1 266 5.21e-81 246
MsaG039204 AT2G40970.1 62.150 214 56 6 1 199 1 204 4.20e-66 207
MsaG039204 AT3G46640.2 37.421 318 135 7 5 272 15 318 3.08e-51 172
MsaG039204 AT3G46640.1 37.421 318 135 7 5 272 15 318 3.08e-51 172
MsaG039204 AT3G46640.3 37.846 325 119 9 5 284 15 301 1.73e-50 170
MsaG039204 AT5G59570.2 46.154 208 65 4 12 184 29 224 6.86e-45 154
MsaG039204 AT5G59570.1 46.154 208 65 4 12 184 29 224 6.86e-45 154
MsaG039204 AT3G16857.3 60.000 75 30 0 79 153 155 229 4.13e-24 102
MsaG039204 AT3G16857.2 60.000 75 30 0 79 153 225 299 5.08e-24 102
MsaG039204 AT3G16857.1 60.000 75 30 0 79 153 225 299 5.43e-24 102
MsaG039204 AT5G58080.1 66.129 62 21 0 88 149 191 252 6.13e-22 96.3
MsaG039204 AT2G01760.3 63.077 65 24 0 90 154 134 198 2.86e-21 92.4
MsaG039204 AT2G01760.1 63.077 65 24 0 90 154 199 263 7.34e-21 92.0
MsaG039204 AT2G25180.1 61.538 65 25 0 90 154 194 258 1.00e-20 92.8
MsaG039204 AT2G01760.2 63.077 65 24 0 90 154 227 291 1.18e-20 91.7
MsaG039204 AT4G31920.1 61.667 60 23 0 90 149 182 241 4.00e-19 87.8
MsaG039204 AT5G07210.1 33.333 144 76 3 82 225 221 344 6.42e-18 84.3
MsaG039204 AT5G07210.2 33.333 144 76 3 82 225 213 336 6.74e-18 84.3
MsaG039204 AT5G44190.1 55.000 60 27 0 90 149 146 205 5.32e-16 78.2
MsaG039204 AT3G62670.1 38.710 124 66 4 90 205 210 331 5.39e-16 78.2
MsaG039204 AT5G44190.2 55.000 60 27 0 90 149 146 205 6.40e-16 77.4
MsaG039204 AT2G20570.1 55.000 60 27 0 90 149 152 211 3.85e-15 75.9
MsaG039204 AT2G20570.2 57.895 57 24 0 93 149 171 227 9.79e-15 74.7
MsaG039204 AT1G67710.3 59.322 59 23 1 93 150 121 179 2.04e-14 73.6
MsaG039204 AT1G67710.2 59.322 59 23 1 93 150 130 188 2.30e-14 73.6
MsaG039204 AT4G18020.8 54.237 59 27 0 91 149 296 354 2.58e-14 73.6
MsaG039204 AT4G18020.11 54.237 59 27 0 91 149 296 354 2.58e-14 73.6
MsaG039204 AT4G18020.4 54.237 59 27 0 91 149 296 354 2.58e-14 73.6
MsaG039204 AT4G18020.5 54.237 59 27 0 91 149 296 354 2.58e-14 73.6
MsaG039204 AT4G18020.10 54.237 59 27 0 91 149 296 354 2.58e-14 73.6
MsaG039204 AT4G18020.7 54.237 59 27 0 91 149 296 354 2.58e-14 73.6
MsaG039204 AT4G18020.3 54.237 59 27 0 91 149 296 354 2.65e-14 73.6
MsaG039204 AT4G18020.2 54.237 59 27 0 91 149 296 354 2.65e-14 73.6
MsaG039204 AT4G18020.1 54.237 59 27 0 91 149 296 354 2.65e-14 73.6
MsaG039204 AT4G18020.6 54.237 59 27 0 91 149 296 354 2.65e-14 73.6
MsaG039204 AT4G18020.9 54.237 59 27 0 91 149 296 354 2.65e-14 73.6
MsaG039204 AT3G12730.1 61.818 55 20 1 91 144 23 77 2.81e-14 71.2
MsaG039204 AT1G67710.1 59.322 59 23 1 93 150 195 253 2.94e-14 73.6
MsaG039204 AT2G27070.1 48.529 68 35 0 82 149 216 283 3.51e-14 73.2
MsaG039204 AT3G04030.2 57.407 54 23 0 91 144 45 98 9.21e-14 71.6
MsaG039204 AT5G45580.1 58.182 55 22 1 91 144 7 61 2.57e-13 68.9
MsaG039204 AT1G79430.2 61.818 55 20 1 91 144 34 88 2.58e-13 70.1
MsaG039204 AT5G45580.2 58.182 55 22 1 91 144 23 77 2.90e-13 69.3
MsaG039204 AT2G03500.1 53.333 60 27 1 90 148 232 291 3.68e-13 69.7
MsaG039204 AT3G04030.1 58.182 55 22 1 91 144 45 99 4.53e-13 69.3
MsaG039204 AT3G04030.3 58.182 55 22 1 91 144 45 99 5.17e-13 69.3
MsaG039204 AT3G24120.2 60.000 55 21 1 91 144 41 95 5.57e-13 68.6
MsaG039204 AT3G24120.1 60.000 55 21 1 91 144 41 95 5.81e-13 68.6
MsaG039204 AT4G13640.2 60.000 55 21 1 91 144 37 91 6.33e-13 68.2
MsaG039204 AT4G13640.1 60.000 55 21 1 91 144 37 91 6.72e-13 68.2
MsaG039204 AT5G18240.2 60.000 55 21 1 91 144 45 99 8.58e-13 68.6
MsaG039204 AT5G18240.3 60.000 55 21 1 91 144 45 99 8.58e-13 68.6
MsaG039204 AT5G18240.5 60.000 55 21 1 91 144 45 99 9.62e-13 68.6
MsaG039204 AT5G18240.1 60.000 55 21 1 91 144 45 99 1.01e-12 68.6
MsaG039204 AT5G18240.4 60.000 55 21 1 91 144 45 99 1.01e-12 68.6
MsaG039204 AT5G49240.1 52.727 55 26 0 86 140 218 272 1.09e-12 67.8
MsaG039204 AT1G13300.1 51.667 60 28 1 90 148 180 239 1.19e-12 67.8
MsaG039204 AT1G25550.1 51.667 60 28 1 90 148 208 267 2.11e-12 67.4
MsaG039204 AT1G49190.1 32.692 104 62 4 90 187 214 315 9.85e-12 65.5
MsaG039204 AT2G01060.1 58.182 55 22 1 91 144 15 69 1.26e-11 64.3
MsaG039204 AT1G68670.1 50.000 62 30 1 88 148 212 273 1.93e-11 64.3
MsaG039204 AT1G49560.1 48.387 62 31 1 88 148 189 250 3.58e-11 63.5
MsaG039204 AT4G37180.1 50.000 60 29 1 90 148 209 268 3.61e-11 63.5
MsaG039204 AT4G37180.2 50.000 60 29 1 90 148 216 275 4.41e-11 63.5
MsaG039204 AT3G13040.3 53.571 56 25 1 90 144 240 295 4.52e-11 63.5
MsaG039204 AT3G13040.1 53.571 56 25 1 90 144 240 295 4.52e-11 63.5
MsaG039204 AT3G13040.2 53.571 56 25 1 90 144 240 295 4.52e-11 63.5

Find 103 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATAGTGGTGGGTGGATT+AGG 0.144441 7:+85926158 None:intergenic
CATCGGCCGGTTGGACATTT+CGG 0.221084 7:-85925636 MsaT039204.1:CDS
CAGCAGCAGCTTCATAGAAT+TGG 0.261093 7:-85925528 MsaT039204.1:CDS
GTGATTAGGGTTTGTGATAA+AGG 0.272510 7:+85926218 None:intergenic
GGATAGTGGTGGGTGGATTA+GGG 0.278062 7:+85926159 None:intergenic
TGAGAGAAGATGATTCAAAT+TGG 0.306698 7:-85926286 MsaT039204.1:CDS
TGGCGAGATCAGGTGTGATT+AGG 0.327778 7:+85926204 None:intergenic
TTCAGTTGAATCGGCGAAAT+CGG 0.335433 7:+85926085 None:intergenic
TCCTGTTGCGCGGCCGAATT+CGG 0.336857 7:-85925727 MsaT039204.1:CDS
TGGTGGTTATGTGGAGGATA+TGG 0.336921 7:-85925463 MsaT039204.1:CDS
ATCGGCCGGTTGGACATTTC+GGG 0.337296 7:-85925635 MsaT039204.1:CDS
AGCAGCAGCTTCATAGAATT+GGG 0.346797 7:-85925527 MsaT039204.1:CDS
CGCATGCAGGGTATTTCTTC+CGG 0.354356 7:-85925846 MsaT039204.1:CDS
GGCGAGATCAGGTGTGATTA+GGG 0.364114 7:+85926205 None:intergenic
GTGGAGGAAGGAAGAGAAGA+AGG 0.372281 7:+85926107 None:intergenic
CGGCGTCGACGAAGCGTTTG+TGG 0.387001 7:+85925988 None:intergenic
TTGTTGTGGTGGTGGGATAG+TGG 0.396749 7:+85926145 None:intergenic
CAATTCTATGAAGCTGCTGC+TGG 0.402280 7:+85925529 None:intergenic
GGTGGTGGTGGTTCCGGTGG+TGG 0.402979 7:-85925822 MsaT039204.1:CDS
TCCGAATTCGGCCGCGCAAC+AGG 0.403622 7:+85925726 None:intergenic
ACTTTAGGTGGTGGTGGTGG+TGG 0.404628 7:-85925486 MsaT039204.1:CDS
TGGTTCTTCGCCGGCGGTGC+CGG 0.408437 7:+85926055 None:intergenic
TGAAGAAAATGCGGCGGTAC+AGG 0.411146 7:+85925750 None:intergenic
CAGGTGCATCATAATACTTT+AGG 0.416308 7:-85925501 MsaT039204.1:CDS
GGCATAAAATGATCCGAATT+CGG 0.417026 7:+85925714 None:intergenic
ACCGGAACCACCACCACCAC+CGG 0.421482 7:+85925827 None:intergenic
TATGAAGCTGCTGCTGGTGC+TGG 0.424890 7:+85925535 None:intergenic
TCGAAAGCGATGGCGAGATC+AGG 0.427672 7:+85926194 None:intergenic
GTTGTTGTTGTTGTGGTGGT+GGG 0.428602 7:+85926138 None:intergenic
GATAAAGGCATGAGTTCTTC+CGG 0.432459 7:+85926233 None:intergenic
TGGAGGAAGGAAGAGAAGAA+GGG 0.433177 7:+85926108 None:intergenic
CATAAGCTGCATTATCGTCT+TGG 0.434900 7:+85925935 None:intergenic
ATTTCTTCCGGTGGTGGTGG+TGG 0.438743 7:-85925834 MsaT039204.1:CDS
TTGTGGTGGTGGGATAGTGG+TGG 0.448450 7:+85926148 None:intergenic
TCAAACGCCCTCGTCTTGTA+TGG 0.450475 7:-85926022 MsaT039204.1:CDS
TCTTTATCTTAAACGCATGC+AGG 0.456959 7:-85925859 MsaT039204.1:CDS
CAGGGTATTTCTTCCGGTGG+TGG 0.460767 7:-85925840 MsaT039204.1:CDS
GGTATTTCTTCCGGTGGTGG+TGG 0.460989 7:-85925837 MsaT039204.1:CDS
CGAAGCGTTTGTGGAGCTGT+GGG 0.467151 7:+85925997 None:intergenic
GTTCTGACTTTGTTTCCAAC+CGG 0.469745 7:-85925411 MsaT039204.1:CDS
TGCTGCTCAGTATCATCGGC+CGG 0.482432 7:-85925649 MsaT039204.1:CDS
GTCGACGCCGTGGCGCATCT+CGG 0.482986 7:-85925975 MsaT039204.1:CDS
GGCATGAGTTCTTCCGGTGG+TGG 0.484319 7:+85926239 None:intergenic
TGATGATGAATAGCAGGAAC+AGG 0.487494 7:+85925684 None:intergenic
ATGGAATCAGCTAATGCAAC+CGG 0.487862 7:-85925444 MsaT039204.1:CDS
ATAAGCTGCATTATCGTCTT+GGG 0.488899 7:+85925936 None:intergenic
AACCCAAATTCAATCATCTC+CGG 0.488992 7:+85925392 None:intergenic
GGATTATCAAAATGTCCATT+CGG 0.494489 7:+85925606 None:intergenic
GAGGAAGGAAGAGAAGAAGG+GGG 0.497445 7:+85926110 None:intergenic
TAATGCAACCGGAGGGAGAA+AGG 0.508295 7:-85925433 MsaT039204.1:CDS
AATACTTTAGGTGGTGGTGG+TGG 0.508543 7:-85925489 MsaT039204.1:CDS
GGAGGTGAGTTCAGTTGAAT+CGG 0.513901 7:+85926076 None:intergenic
TGCTGTTGATGATGAATAGC+AGG 0.520424 7:+85925678 None:intergenic
GGCGACCCGAAATGTCCAAC+CGG 0.526706 7:+85925630 None:intergenic
GGAGGAAGGAAGAGAAGAAG+GGG 0.527059 7:+85926109 None:intergenic
AGTCAGAACCTTTCTCCCTC+CGG 0.527350 7:+85925425 None:intergenic
CTGCATTATCGTCTTGGGAA+CGG 0.529968 7:+85925941 None:intergenic
ACGAAGCGTTTGTGGAGCTG+TGG 0.532297 7:+85925996 None:intergenic
CATAATACTTTAGGTGGTGG+TGG 0.535349 7:-85925492 MsaT039204.1:CDS
TGAATCGGCGAAATCGGTGG+AGG 0.543830 7:+85926091 None:intergenic
TTCTTCGCCGGCGGTGCCGG+AGG 0.545793 7:+85926058 None:intergenic
CATCATAATACTTTAGGTGG+TGG 0.547552 7:-85925495 MsaT039204.1:CDS
GAACTCACCTCCGGCACCGC+CGG 0.547744 7:-85926065 MsaT039204.1:CDS
TGTGGTGGTGGGATAGTGGT+GGG 0.548736 7:+85926149 None:intergenic
GCTTCATAGAATTGGGGCAC+AGG 0.550758 7:-85925520 MsaT039204.1:CDS
GCTCAGTATCATCGGCCGGT+TGG 0.552483 7:-85925645 MsaT039204.1:CDS
AGTTGAATCGGCGAAATCGG+TGG 0.557119 7:+85926088 None:intergenic
GAAGAAAATGCGGCGGTACA+GGG 0.558776 7:+85925751 None:intergenic
GGGCGTTTGAGTGTGCGAGC+TGG 0.560084 7:+85926035 None:intergenic
CTTTATCTTAAACGCATGCA+GGG 0.564649 7:-85925858 MsaT039204.1:CDS
CATTTCGGGTCGCCGCCGAA+TGG 0.568846 7:-85925621 MsaT039204.1:CDS
TGTGCGAGCTGGTTCTTCGC+CGG 0.569228 7:+85926046 None:intergenic
TCGGCGAAATCGGTGGAGGA+AGG 0.569986 7:+85926095 None:intergenic
ATAGCAGGAACAGGAACAAA+CGG 0.570737 7:+85925693 None:intergenic
AACAGAAGCAGAACCACCAC+CGG 0.574953 7:+85925809 None:intergenic
GTTTCGTATGTCGAAAGCGA+TGG 0.584109 7:+85926184 None:intergenic
AAGAAAATGCGGCGGTACAG+GGG 0.587168 7:+85925752 None:intergenic
ATGCAGGGTATTTCTTCCGG+TGG 0.591915 7:-85925843 MsaT039204.1:CDS
CAAATTCAATCATCTCCGGT+TGG 0.598529 7:+85925396 None:intergenic
GGAAGAACAACTCCCACCAC+CGG 0.598745 7:-85926252 MsaT039204.1:CDS
ATGCAGCTTATGAGTGTGGA+TGG 0.599001 7:-85925924 MsaT039204.1:CDS
GGTGGTGGGATAGTGGTGGG+TGG 0.599530 7:+85926152 None:intergenic
GCATGAGTTCTTCCGGTGGT+GGG 0.599774 7:+85926240 None:intergenic
GCGAGCTGGTTCTTCGCCGG+CGG 0.605171 7:+85926049 None:intergenic
TATTCATCATCAACAGCAGA+TGG 0.608957 7:-85925673 MsaT039204.1:CDS
TGGCTGCTGCTCAGTATCAT+CGG 0.613909 7:-85925653 MsaT039204.1:CDS
TGGTGGTGGTGGTTATGTGG+AGG 0.625699 7:-85925469 MsaT039204.1:CDS
CTTGATACCGAGATGCGCCA+CGG 0.632900 7:+85925968 None:intergenic
GTGCATCATAATACTTTAGG+TGG 0.635035 7:-85925498 MsaT039204.1:CDS
GAATCAGCTAATGCAACCGG+AGG 0.639788 7:-85925441 MsaT039204.1:CDS
GATTCAACTGAACTCACCTC+CGG 0.640353 7:-85926074 MsaT039204.1:CDS
TGGTTTAACGAGAGAAAACG+TGG 0.642801 7:-85925904 MsaT039204.1:CDS
GCAGCAGCTTCATAGAATTG+GGG 0.666624 7:-85925526 MsaT039204.1:CDS
TGTGGGGTCCATACAAGACG+AGG 0.673250 7:+85926014 None:intergenic
CAAACGCTTCGTCGACGCCG+TGG 0.674680 7:-85925985 MsaT039204.1:CDS
GATAATGCAGCTTATGAGTG+TGG 0.675001 7:-85925928 MsaT039204.1:CDS
GCAACAGGATGAAGAAAATG+CGG 0.684941 7:+85925741 None:intergenic
GAAGCGTTTGTGGAGCTGTG+GGG 0.695777 7:+85925998 None:intergenic
TTATCAAAATGTCCATTCGG+CGG 0.700387 7:+85925609 None:intergenic
AATCAGCTAATGCAACCGGA+GGG 0.707357 7:-85925440 MsaT039204.1:CDS
GTGGGGTCCATACAAGACGA+GGG 0.708697 7:+85926015 None:intergenic
ACAGGATGAAGAAAATGCGG+CGG 0.721657 7:+85925744 None:intergenic
AAAGGCATGAGTTCTTCCGG+TGG 0.729666 7:+85926236 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 85925401 85926309 85925401 ID=MsaG039204
Chr7 mRNA 85925401 85926309 85925401 ID=MsaT039204.1;Parent=MsaG039204
Chr7 exon 85925401 85926309 85925401 ID=MsaT039204.1.exon1;Parent=MsaT039204.1
Chr7 CDS 85925401 85926309 85925401 ID=cds.MsaT039204.1;Parent=MsaT039204.1
Gene Sequence

>MsaT039204.1

ATGAGAGAAGATGATTCAAATTGGTTTTCAAGATGGGAAGAACAACTCCCACCACCGGAAGAACTCATGCCTTTATCACAAACCCTAATCACACCTGATCTCGCCATCGCTTTCGACATACGAAACAACCCTAATCCACCCACCACTATCCCACCACCACAACAACAACAACAACCACCCCCTTCTTCTCTTCCTTCCTCCACCGATTTCGCCGATTCAACTGAACTCACCTCCGGCACCGCCGGCGAAGAACCAGCTCGCACACTCAAACGCCCTCGTCTTGTATGGACCCCACAGCTCCACAAACGCTTCGTCGACGCCGTGGCGCATCTCGGTATCAAGAACGCCGTTCCCAAGACGATAATGCAGCTTATGAGTGTGGATGGTTTAACGAGAGAAAACGTGGCGAGTCATCTTCAGAAATATCGTCTTTATCTTAAACGCATGCAGGGTATTTCTTCCGGTGGTGGTGGTGGTTCCGGTGGTGGTTCTGCTTCTGTTAATGATGCTGCTACTGATCATTTATTTGCTAGCTCCCCTGTACCGCCGCATTTTCTTCATCCTGTTGCGCGGCCGAATTCGGATCATTTTATGCCGTTTGTTCCTGTTCCTGCTATTCATCATCAACAGCAGATGGCTGCTGCTCAGTATCATCGGCCGGTTGGACATTTCGGGTCGCCGCCGAATGGACATTTTGATAATCCTTTTTTGTCTAGACAGCAACATCATCAACAGCAAAATCAGCATCAACACCAGCACCAGCAGCAGCTTCATAGAATTGGGGCACAGGTGCATCATAATACTTTAGGTGGTGGTGGTGGTGGTGGTGGTTATGTGGAGGATATGGAATCAGCTAATGCAACCGGAGGGAGAAAGGTTCTGACTTTGTTTCCAACCGGAGATGATTGA
Protein sequence

>MsaT039204.1

MREDDSNWFSRWEEQLPPPEELMPLSQTLITPDLAIAFDIRNNPNPPTTIPPPQQQQQPPPSSLPSSTDFADSTELTSGTAGEEPARTLKRPRLVWTPQLHKRFVDAVAHLGIKNAVPKTIMQLMSVDGLTRENVASHLQKYRLYLKRMQGISSGGGGGSGGGSASVNDAATDHLFASSPVPPHFLHPVARPNSDHFMPFVPVPAIHHQQQMAAAQYHRPVGHFGSPPNGHFDNPFLSRQQHHQQQNQHQHQHQQQLHRIGAQVHHNTLGGGGGGGGYVEDMESANATGGRKVLTLFPTGDD*