AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG039214


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039214 MtrunA17_Chr7g0255841 100.000 92 0 0 1 92 1 92 7.13e-65 190
MsaG039214 MtrunA17_Chr3g0081631 72.222 90 24 1 4 92 2 91 1.26e-44 139
MsaG039214 MtrunA17_Chr6g0449111 72.059 68 19 0 7 74 5 72 6.26e-35 114
MsaG039214 MtrunA17_Chr3g0144631 68.354 79 21 1 1 75 1 79 5.32e-34 112
MsaG039214 MtrunA17_Chr1g0155181 59.302 86 29 1 6 85 1 86 6.14e-33 109
MsaG039214 MtrunA17_Chr3g0141601 57.143 91 35 2 6 92 1 91 3.14e-32 107
MsaG039214 MtrunA17_Chr5g0404101 63.014 73 27 0 4 76 11 83 1.24e-30 103
MsaG039214 MtrunA17_Chr8g0373131 56.716 67 28 1 9 75 2 67 7.81e-25 88.6
MsaG039214 MtrunA17_Chr8g0373111 56.716 67 28 1 9 75 2 67 8.21e-25 88.6
MsaG039214 MtrunA17_Chr8g0373121 56.716 67 28 1 9 75 2 67 8.21e-25 88.6
MsaG039214 MtrunA17_Chr3g0141641 45.055 91 41 2 11 92 2 92 3.32e-24 87.4
MsaG039214 MtrunA17_Chr5g0404301 60.317 63 24 1 10 72 2 63 9.33e-24 85.5
MsaG039214 MtrunA17_Chr8g0373101 56.452 62 26 1 11 72 4 64 6.78e-23 83.6
MsaG039214 MtrunA17_Chr5g0436501 54.930 71 32 0 12 82 27 97 1.30e-20 82.8
MsaG039214 MtrunA17_Chr8g0364741 50.667 75 37 0 6 80 4 78 2.62e-20 78.6
MsaG039214 MtrunA17_Chr1g0162081 50.000 78 39 0 1 78 1 78 1.38e-19 80.5
MsaG039214 MtrunA17_Chr8g0365011 54.167 72 33 0 9 80 5 76 2.84e-19 79.3
MsaG039214 MtrunA17_Chr3g0104071 50.685 73 36 0 2 74 33 105 9.65e-19 78.2
MsaG039214 MtrunA17_Chr5g0429521 47.826 69 36 0 11 79 28 96 5.02e-18 76.3
MsaG039214 MtrunA17_Chr7g0241761 52.174 69 33 0 12 80 35 103 5.19e-18 76.3
MsaG039214 MtrunA17_Chr1g0194071 57.627 59 24 1 11 68 10 68 6.75e-16 66.6
MsaG039214 MtrunA17_Chr1g0210471 47.619 63 33 0 12 74 24 86 7.82e-15 67.0
MsaG039214 MtrunA17_Chr7g0270711 55.000 60 26 1 11 69 10 69 3.19e-14 62.0
MsaG039214 MtrunA17_Chr4g0062741 45.205 73 36 2 6 74 1 73 4.56e-14 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039214 AT2G21650.1 64.773 88 24 1 12 92 14 101 4.16e-38 123
MsaG039214 AT4G39250.1 64.368 87 25 1 12 92 14 100 1.32e-37 122
MsaG039214 AT1G75250.2 55.670 97 37 1 1 91 1 97 5.60e-36 117
MsaG039214 AT1G75250.1 69.333 75 23 0 1 75 1 75 1.47e-35 117
MsaG039214 AT1G19510.1 59.783 92 37 0 1 92 1 92 2.64e-29 100
MsaG039214 AT2G18328.1 62.295 61 23 0 15 75 14 74 1.84e-24 88.2
MsaG039214 AT5G05790.2 52.857 70 33 0 9 78 28 97 3.54e-20 82.0
MsaG039214 AT5G05790.1 52.857 70 33 0 9 78 28 97 3.54e-20 82.0
MsaG039214 AT4G36570.1 75.000 44 11 0 12 55 12 55 4.92e-20 76.3
MsaG039214 AT3G11280.2 52.239 67 32 0 12 78 33 99 1.03e-18 77.8
MsaG039214 AT3G11280.1 52.239 67 32 0 12 78 33 99 1.03e-18 77.8
MsaG039214 AT2G38090.1 52.174 69 33 0 12 80 29 97 2.27e-18 77.4
MsaG039214 AT5G58900.1 50.000 74 34 1 4 74 23 96 3.03e-17 74.3
MsaG039214 AT5G08520.1 52.381 63 30 0 12 74 12 74 5.44e-16 70.9
MsaG039214 AT1G49010.1 46.479 71 35 1 7 74 3 73 1.43e-14 67.4
MsaG039214 AT5G23650.1 46.377 69 37 0 6 74 7 75 2.01e-14 67.0
MsaG039214 AT5G04760.1 41.791 67 38 1 8 74 2 67 4.55e-13 62.4
MsaG039214 AT5G01200.1 42.424 66 37 1 10 74 28 93 2.05e-11 58.2

Find 16 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAGTTCAATGTCAGCTTC+TGG 0.381239 7:-86115306 MsaT039214.1:CDS
GATATTAAACATATTGAGTC+AGG 0.394581 7:-86115135 MsaT039214.1:CDS
TAATGTTTCTTAACTTCCTC+AGG 0.405406 7:+86115174 None:intergenic
CAGTGTCTCATGAGGAGAAA+AGG 0.467404 7:-86115080 MsaT039214.1:intron
CAATGTCAGCTTCTGGCTCA+TGG 0.480309 7:-86115299 MsaT039214.1:CDS
TTCTGGCTCATGGAGTGTTA+AGG 0.488975 7:-86115289 MsaT039214.1:CDS
TTGCACTTGTTGGTCAATGA+AGG 0.513934 7:+86114859 None:intergenic
GCTACATTGTACCAACGATC+TGG 0.520197 7:+86115219 None:intergenic
AACAGCTAAAGCTCTTTCAA+AGG 0.549456 7:+86115257 None:intergenic
ACATTATGAACTTCTTGTTG+AGG 0.570098 7:-86115157 MsaT039214.1:CDS
GAAAATTTCAGTGTCTCATG+AGG 0.590467 7:-86115088 MsaT039214.1:CDS
TACAATGTAGCTCATGCTGT+TGG 0.593737 7:-86115207 MsaT039214.1:CDS
AATGTAGCTCATGCTGTTGG+TGG 0.602491 7:-86115204 MsaT039214.1:CDS
ACAAAGACACTCCAGATCGT+TGG 0.612332 7:-86115230 MsaT039214.1:CDS
ATATTAAACATATTGAGTCA+GGG 0.667795 7:-86115134 MsaT039214.1:CDS
TGTTGGTGGCAAAACTCCTG+AGG 0.702099 7:-86115190 MsaT039214.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 86114872 86115334 86114872 ID=MsaG039214
Chr7 mRNA 86114872 86115334 86114872 ID=MsaT039214.1;Parent=MsaG039214
Chr7 exon 86114872 86114896 86114872 ID=MsaT039214.1.exon2;Parent=MsaT039214.1
Chr7 CDS 86114872 86114896 86114872 ID=cds.MsaT039214.1;Parent=MsaT039214.1
Chr7 exon 86115081 86115334 86115081 ID=MsaT039214.1.exon1;Parent=MsaT039214.1
Chr7 CDS 86115081 86115334 86115081 ID=cds.MsaT039214.1;Parent=MsaT039214.1
Gene Sequence

>MsaT039214.1

ATGGCATCTAGTTCAATGTCAGCTTCTGGCTCATGGAGTGTTAAGGAGAACAAAGCCTTTGAAAGAGCTTTAGCTGTTTATGACAAAGACACTCCAGATCGTTGGTACAATGTAGCTCATGCTGTTGGTGGCAAAACTCCTGAGGAAGTTAAGAAACATTATGAACTTCTTGTTGAGGATATTAAACATATTGAGTCAGGGAAAGTTCCATTTCCAAATTACAAGAAAATTTCAGTGTCTCATGAGGAGAAAAGGATGAGGAACATGAGCCTTCATTGA

Protein sequence

>MsaT039214.1

MASSSMSASGSWSVKENKAFERALAVYDKDTPDRWYNVAHAVGGKTPEEVKKHYELLVEDIKHIESGKVPFPNYKKISVSHEEKRMRNMSLH*