Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039220 | XP_003607052.1 | 78.523 | 149 | 32 | 0 | 1 | 149 | 1 | 149 | 8.52e-81 | 245 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039220 | sp|F4JZT3|ARR24_ARATH | 32.381 | 105 | 71 | 0 | 33 | 137 | 18 | 122 | 1.35e-15 | 72.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039220 | tr|G7JJU6|G7JJU6_MEDTR | 78.523 | 149 | 32 | 0 | 1 | 149 | 1 | 149 | 4.07e-81 | 245 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG039220 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039220 | MtrunA17_Chr6g0451941 | 78.523 | 149 | 32 | 0 | 1 | 149 | 1 | 149 | 7.84e-85 | 245 |
| MsaG039220 | MtrunA17_Chr8g0364781 | 31.304 | 115 | 77 | 1 | 33 | 145 | 12 | 126 | 8.60e-15 | 67.0 |
| MsaG039220 | MtrunA17_Chr7g0262351 | 30.973 | 113 | 77 | 1 | 25 | 137 | 14 | 125 | 1.22e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039220 | AT5G26594.1 | 32.381 | 105 | 71 | 0 | 33 | 137 | 18 | 122 | 1.37e-16 | 72.0 |
Find 22 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAAACTTTCTTTGGTTAA+AGG | 0.152490 | 7:+86170815 | None:intergenic |
| CTTTCTTTGGTTAAAGGTTT+TGG | 0.180470 | 7:+86170821 | None:intergenic |
| CTCTTTCATTTGTTTATATC+TGG | 0.180960 | 7:+86172287 | None:intergenic |
| TACCTGGGGTCCATTGATTA+TGG | 0.224477 | 7:+86172041 | None:intergenic |
| GACCAGTTCAAAACTTTCTT+TGG | 0.249411 | 7:+86170808 | None:intergenic |
| TAGCACCAGTCAATAAAATA+TGG | 0.283273 | 7:+86172097 | None:intergenic |
| GCTTCTTGAGAACTATTTGC+AGG | 0.359227 | 7:+86172123 | None:intergenic |
| ATCAATGCATTGCTAATAGA+TGG | 0.376367 | 7:-86172207 | MsaT039220.1:CDS |
| CATCAAATGCTTGAATCAAT+AGG | 0.381106 | 7:-86172159 | MsaT039220.1:CDS |
| TTTGATTTGTCCATAATCAA+TGG | 0.400791 | 7:-86172051 | MsaT039220.1:CDS |
| TTTCATTTGTTTATATCTGG+TGG | 0.469134 | 7:+86172290 | None:intergenic |
| GTGAGACAGATGCGTGCAAT+GGG | 0.473335 | 7:-86170932 | MsaT039220.1:CDS |
| AACATGAAATTCATGTGTAT+CGG | 0.526904 | 7:+86170774 | None:intergenic |
| AGCAATGCATTGATGAGATA+AGG | 0.528514 | 7:+86172216 | None:intergenic |
| TGAGACTGCAACGAGACGCT+TGG | 0.539108 | 7:-86172184 | MsaT039220.1:CDS |
| GTCCATAATCAATGGACCCC+AGG | 0.541606 | 7:-86172043 | MsaT039220.1:intron |
| ATTGCACATACAATGGATGC+AGG | 0.592854 | 7:-86172315 | None:intergenic |
| ATGAAATTCATGTGTATCGG+AGG | 0.606494 | 7:+86170777 | None:intergenic |
| GGTGAGACAGATGCGTGCAA+TGG | 0.623881 | 7:-86170933 | MsaT039220.1:CDS |
| GGGATACAGAGCAAGATGGT+CGG | 0.640760 | 7:-86170911 | MsaT039220.1:CDS |
| AATGGGGATACAGAGCAAGA+TGG | 0.668118 | 7:-86170915 | MsaT039220.1:CDS |
| TGAGACAGATGCGTGCAATG+GGG | 0.812421 | 7:-86170931 | MsaT039220.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 86170784 | 86172325 | 86170784 | ID=MsaG039220 |
| Chr7 | mRNA | 86170784 | 86172325 | 86170784 | ID=MsaT039220.1;Parent=MsaG039220 |
| Chr7 | exon | 86170784 | 86170957 | 86170784 | ID=MsaT039220.1.exon2;Parent=MsaT039220.1 |
| Chr7 | CDS | 86170784 | 86170957 | 86170784 | ID=cds.MsaT039220.1;Parent=MsaT039220.1 |
| Chr7 | exon | 86172044 | 86172325 | 86172044 | ID=MsaT039220.1.exon1;Parent=MsaT039220.1 |
| Chr7 | CDS | 86172044 | 86172325 | 86172044 | ID=cds.MsaT039220.1;Parent=MsaT039220.1 |
| Gene Sequence |
| Protein sequence |
