AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG039394

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039394 MtrunA17_Chr7g0257831 97.956 734 15 0 1 734 1 734 0.0 1480
MsaG039394 MtrunA17_Chr3g0088521 64.485 718 219 16 33 731 31 731 0.0 845
MsaG039394 MtrunA17_Chr1g0171151 54.355 574 239 11 33 601 17 572 0.0 565
MsaG039394 MtrunA17_Chr5g0437251 42.647 748 297 24 34 731 18 683 1.55e-163 488
MsaG039394 MtrunA17_Chr5g0430211 41.715 688 299 16 39 678 27 660 9.20e-154 463
MsaG039394 MtrunA17_Chr1g0187591 41.420 676 299 19 44 680 30 647 4.25e-142 431
MsaG039394 MtrunA17_Chr3g0092171 53.528 411 149 8 16 422 56 428 3.43e-133 403
MsaG039394 MtrunA17_Chr3g0092171 60.377 53 21 0 623 675 453 505 2.35e-12 70.1
MsaG039394 MtrunA17_Chr3g0092221 50.124 403 143 10 95 495 12 358 1.06e-110 341
MsaG039394 MtrunA17_Chr3g0113651 40.132 456 160 12 45 490 8 360 7.45e-96 306
MsaG039394 MtrunA17_Chr3g0105131 35.940 601 245 18 62 661 32 493 1.78e-95 305
MsaG039394 MtrunA17_Chr3g0105101 40.812 468 173 11 50 517 28 391 7.98e-95 300
MsaG039394 MtrunA17_Chr3g0105141 66.929 127 41 1 243 368 15 141 4.93e-61 202
MsaG039394 MtrunA17_Chr6g0487481 75.701 107 26 0 260 366 99 205 2.24e-57 199
MsaG039394 MtrunA17_Chr8g0389561 57.229 166 57 2 260 417 77 236 2.81e-56 197
MsaG039394 MtrunA17_Chr3g0105121 34.123 422 196 13 263 675 8 356 6.35e-54 189
MsaG039394 MtrunA17_Chr2g0324781 69.369 111 34 0 264 374 77 187 1.25e-53 191
MsaG039394 MtrunA17_Chr3g0135351 61.029 136 49 2 254 388 43 175 1.70e-52 183
MsaG039394 MtrunA17_Chr4g0040391 66.355 107 36 0 260 366 56 162 1.54e-50 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039394 AT2G41900.1 65.263 760 194 21 1 734 1 716 0.0 873
MsaG039394 AT5G12850.1 53.607 707 271 19 41 727 36 705 0.0 681
MsaG039394 AT5G58620.1 44.678 667 254 18 44 683 11 589 1.28e-161 480
MsaG039394 AT2G40140.2 40.311 707 257 20 43 728 29 591 7.70e-143 432
MsaG039394 AT2G40140.1 40.311 707 257 20 43 728 29 591 7.70e-143 432
MsaG039394 AT3G55980.1 47.816 435 155 9 41 461 24 400 5.28e-124 382
MsaG039394 AT3G55980.2 47.816 435 155 9 41 461 91 467 6.32e-123 382
MsaG039394 AT4G29190.1 56.284 183 69 3 253 428 68 246 1.21e-58 203
MsaG039394 AT2G25900.2 72.477 109 30 0 260 368 86 194 9.80e-57 196
MsaG039394 AT2G25900.1 72.477 109 30 0 260 368 86 194 9.80e-57 196
MsaG039394 AT2G19810.1 68.033 122 37 1 249 368 61 182 2.27e-56 197
MsaG039394 AT5G07500.1 64.463 121 43 0 246 366 34 154 1.23e-54 188
MsaG039394 AT1G03790.1 59.859 142 49 3 242 379 64 201 7.99e-52 185
MsaG039394 AT5G44260.1 60.156 128 46 2 242 368 42 165 1.16e-48 176

Find 197 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATAATTCTTGGTCAAAA+TGG 0.154209 7:+88591973 MsaT039394.1:CDS
CTAGTGCTGGCTGTAATCTT+AGG 0.193223 7:+88590668 MsaT039394.1:CDS
GAAATTGATTATTTCTTTAT+CGG 0.251530 7:+88590417 MsaT039394.1:CDS
GTGAAGCTGCTTCTTGCAGC+AGG 0.273866 7:+88590529 MsaT039394.1:CDS
CCGTTAGCATCCACAGAATT+AGG 0.277880 7:-88590559 None:intergenic
AACTGAGATCAAAGAGAAAT+TGG 0.283003 7:+88592136 MsaT039394.1:CDS
TTGATGGTTGCTGCTACTTA+TGG 0.292112 7:+88590379 MsaT039394.1:CDS
AACTCTATGCTTGCAGCTGC+TGG 0.296806 7:+88591945 MsaT039394.1:CDS
AAAACAGTTGAAGTCAGATT+TGG 0.296997 7:-88591663 None:intergenic
TTCTTACTCTCCTCCTTTGT+CGG 0.299145 7:+88590711 MsaT039394.1:CDS
TACAATGGCCGAAGCTCTTC+AGG 0.319804 7:-88591183 None:intergenic
ATCCCAGCGTTGCATCTTCC+TGG 0.323243 7:+88591399 MsaT039394.1:CDS
TTATTGCAGGCAGCTTCTTA+TGG 0.334502 7:+88591789 MsaT039394.1:CDS
ATCTCAGGTGCTGCGCCAGC+TGG 0.340015 7:+88592167 MsaT039394.1:CDS
GCAAGGGTGCTTGCCGGCGC+GGG 0.345602 7:+88591040 MsaT039394.1:CDS
CGGTGGATGAGGTTGGATTA+TGG 0.347533 7:+88590305 MsaT039394.1:CDS
GCTCTTCACTGTGCTGCTTC+TGG 0.348886 7:+88590481 MsaT039394.1:CDS
GCAGCCATGAGCATGTTGCC+TGG 0.353518 7:+88591270 MsaT039394.1:CDS
TGCTGCGCCAGCTGGTTCCT+CGG 0.353578 7:+88592175 MsaT039394.1:CDS
CTGGCTCAGTCAATAATTCT+TGG 0.360024 7:+88591964 MsaT039394.1:CDS
ACAGGCATGCCAACTCATTA+AGG 0.364336 7:-88591524 None:intergenic
CGGCCATTGTATGTTTCAAC+TGG 0.366588 7:+88591195 MsaT039394.1:CDS
AAGAGATCATCAAGATTTGA+TGG 0.375352 7:-88591606 None:intergenic
TCAAGATTTGATGGATTCAA+TGG 0.378043 7:-88591597 None:intergenic
CACGTGCTTATTCCCATGAT+TGG 0.379732 7:+88590926 MsaT039394.1:CDS
ACCATGAATCACTTAACTAT+TGG 0.382333 7:+88590193 MsaT039394.1:CDS
GATGTCCCTCTTTCTCCTGC+TGG 0.388703 7:+88590796 MsaT039394.1:CDS
ATTCCGAATGTATTCTTTCA+AGG 0.389840 7:+88590891 MsaT039394.1:CDS
CTAATTCTGTGGATGCTAAC+GGG 0.391198 7:+88590560 MsaT039394.1:CDS
CTAGACTGAAGATTACTTCC+AGG 0.391284 7:-88591417 None:intergenic
TATCGGATGTTGATATCAAC+AGG 0.399105 7:+88590434 MsaT039394.1:CDS
ACCAATAGTTAAGTGATTCA+TGG 0.401667 7:-88590194 None:intergenic
GAGGAGCTACTTCAAATTGA+TGG 0.405740 7:+88590643 MsaT039394.1:CDS
TTCACTGTGCTGCTTCTGGT+GGG 0.407537 7:+88590485 MsaT039394.1:CDS
CGAGCAAAGGACGCCTTTGA+TGG 0.407819 7:+88590363 MsaT039394.1:CDS
GATCATCTTCATTTGAGCTT+GGG 0.407859 7:+88592057 MsaT039394.1:CDS
CAAGCACCCTTGCGAAAATC+AGG 0.409349 7:-88591030 None:intergenic
GCCAGCTGGTTCCTCGGGTG+AGG 0.415311 7:+88592181 MsaT039394.1:CDS
TGTCGGCTTCACCTGAGAAC+GGG 0.415939 7:+88590728 MsaT039394.1:CDS
TGAAGCTGCTTCTTGCAGCA+GGG 0.417923 7:+88590530 MsaT039394.1:CDS
TGATTCATGGTGTGGTTTCT+CGG 0.418912 7:-88590181 None:intergenic
CAATTGACTTCAACTTCAAC+AGG 0.419193 7:-88590774 None:intergenic
AGATGCAACGCTGGGATGTT+TGG 0.421817 7:-88591393 None:intergenic
TTTCACACAACTCTGTTGCT+TGG 0.421951 7:+88591364 MsaT039394.1:CDS
CAATAATTCTTGGTCAAAAT+GGG 0.422094 7:+88591974 MsaT039394.1:CDS
GTCTGTTGATCATGCTGTAT+TGG 0.422918 7:+88592229 MsaT039394.1:CDS
CTTGAGCCCATCGGAGAGAT+AGG 0.425600 7:-88591852 None:intergenic
TCTGTTGATCATGCTGTATT+GGG 0.426592 7:+88592230 MsaT039394.1:CDS
CAGCTGTAATGATACTTTCT+CGG 0.429242 7:-88590997 None:intergenic
GCCCAATCCAACTTACCATT+GGG 0.431247 7:-88592002 None:intergenic
TCTTATGGTGTTCAGCCATC+AGG 0.437466 7:+88591804 MsaT039394.1:CDS
AAGGACATTCAGTCCAATCA+TGG 0.439456 7:-88590939 None:intergenic
CGCAAGGGTGCTTGCCGGCG+CGG 0.442685 7:+88591039 MsaT039394.1:CDS
GCTGCGCCAGCTGGTTCCTC+GGG 0.442943 7:+88592176 MsaT039394.1:CDS
GACTGCACCCATGATAAATC+AGG 0.445095 7:-88592095 None:intergenic
AATTTCTCTTTGATCTCAGT+TGG 0.445539 7:-88592134 None:intergenic
CGTGGAGACATTCTTCCTGA+TGG 0.449642 7:-88591819 None:intergenic
ACTCCACCCATGTCACCTTC+TGG 0.450662 7:+88591333 MsaT039394.1:CDS
GGCAAAGAAGCAAACTCTCC+TGG 0.454696 7:-88591154 None:intergenic
CCCCAATGGTAAGTTGGATT+GGG 0.458669 7:+88592001 MsaT039394.1:CDS
TGTTGATCATCATTTATTGC+AGG 0.464841 7:+88591776 MsaT039394.1:CDS
TTCTCCCCTGGATGAACAAA+AGG 0.464994 7:-88590958 None:intergenic
TCAGGAAGGGATGGATCAAC+AGG 0.466570 7:-88590838 None:intergenic
GACATCGAAGAAGGTGAGCC+AGG 0.468116 7:-88591288 None:intergenic
AGATCATCTTCATTTGAGCT+TGG 0.468995 7:+88592056 MsaT039394.1:CDS
TAGTGCTGGCTGTAATCTTA+GGG 0.470828 7:+88590669 MsaT039394.1:CDS
TTCAACTTCAACAGGAGATC+CGG 0.470845 7:-88590766 None:intergenic
AATTAATATGACGTGGAGAC+AGG 0.471650 7:-88591542 None:intergenic
TTGTCGGCTTCACCTGAGAA+CGG 0.475746 7:+88590727 MsaT039394.1:CDS
TGATCCTACGTCGGTGGATG+AGG 0.478013 7:+88590294 MsaT039394.1:CDS
AATGAATATGAGCACACAAA+TGG 0.478801 7:+88592205 MsaT039394.1:CDS
GCGAGGAGCCTGATTTATCA+TGG 0.480265 7:+88592087 MsaT039394.1:CDS
CTTCACTGTGCTGCTTCTGG+TGG 0.482790 7:+88590484 MsaT039394.1:CDS
TTCATCCAGGGGAGAATGCT+CGG 0.483091 7:+88590965 MsaT039394.1:CDS
AGGAGATCCGGTGGTGGAGA+AGG 0.485489 7:-88590754 None:intergenic
GGAGAAGGAGACCCGTTCTC+AGG 0.488091 7:-88590739 None:intergenic
GGTGACATGGGTGGAGTGAA+TGG 0.492460 7:-88591327 None:intergenic
CTTCTCCGAGCATTCTCCCC+TGG 0.492674 7:-88590970 None:intergenic
GTCAAGCACGTCTTCTGCCA+TGG 0.494988 7:+88591239 MsaT039394.1:CDS
AAATTGGCAACTTCCATCTC+AGG 0.496278 7:+88592152 MsaT039394.1:CDS
GAGATAGGTTCCACGTTTCG+TGG 0.497212 7:-88591837 None:intergenic
CCAGCTGGTTCCTCGGGTGA+GGG 0.498286 7:+88592182 MsaT039394.1:CDS
AGCTGGCGCAGCACCTGAGA+TGG 0.500158 7:-88592165 None:intergenic
AGCCCAATCCAACTTACCAT+TGG 0.500923 7:-88592003 None:intergenic
CAATGCTAGAGATATTCATA+TGG 0.504807 7:+88591458 MsaT039394.1:CDS
CCCACACTCCTGAAGAGCTT+CGG 0.505759 7:+88591175 MsaT039394.1:CDS
TTGTCCCGTCCTTGCAGAGA+CGG 0.505915 7:-88591125 None:intergenic
GCTGCTAATAACGACGTTGA+AGG 0.506533 7:+88590250 MsaT039394.1:CDS
TGTCCCGTCCTTGCAGAGAC+GGG 0.508427 7:-88591124 None:intergenic
CCTAATTCTGTGGATGCTAA+CGG 0.509817 7:+88590559 MsaT039394.1:CDS
AGCTGTAATGATACTTTCTC+GGG 0.515430 7:-88590996 None:intergenic
CTATCTCTCCGATGGGCTCA+AGG 0.517086 7:+88591853 MsaT039394.1:CDS
TCAGATCCAGCAGGAGAAAG+AGG 0.517866 7:-88590802 None:intergenic
CAAATTGATGGTCCTAGTGC+TGG 0.518193 7:+88590655 MsaT039394.1:CDS
GGACCTTGAAAGAATACATT+CGG 0.520381 7:-88590894 None:intergenic
TGAGAATTGTGTGTCAAAGT+TGG 0.523284 7:-88590157 None:intergenic
CCCTCACCCGAGGAACCAGC+TGG 0.525483 7:-88592182 None:intergenic
TCTTTCTTCTCAGATCCAGC+AGG 0.526498 7:-88590811 None:intergenic
GACATGTGTGAATATGCTCA+TGG 0.527602 7:+88591063 MsaT039394.1:CDS
CCTACGTCGGTGGATGAGGT+TGG 0.530222 7:+88590298 MsaT039394.1:CDS
TCTAACAGTCTTAGCCGGTC+TGG 0.533057 7:+88591564 MsaT039394.1:CDS
CGGGTCTCCTTCTCCACCAC+CGG 0.535005 7:+88590747 MsaT039394.1:CDS
GAAGCTGCTTCTTGCAGCAG+GGG 0.537195 7:+88590531 MsaT039394.1:CDS
AAGACAGCTGATTTGTGAGT+CGG 0.538872 7:-88591690 None:intergenic
CTGCTAATAACGACGTTGAA+GGG 0.540153 7:+88590251 MsaT039394.1:CDS
AAGGATGTGCTGTGAGTTGG+AGG 0.541019 7:+88590096 None:intergenic
CTAAGATTACAGCCAGCACT+AGG 0.544045 7:-88590667 None:intergenic
CAGGGGAGAATGCTCGGAGA+AGG 0.544336 7:+88590971 MsaT039394.1:CDS
TCATTTGAGCTTGGGAACAA+TGG 0.545835 7:+88592065 MsaT039394.1:CDS
GGGCTCAAGGATGTCAATGC+TGG 0.547672 7:+88591866 MsaT039394.1:CDS
GCCGAAGCTCTTCAGGAGTG+TGG 0.548910 7:-88591176 None:intergenic
CAAGGGTGCTTGCCGGCGCG+GGG 0.549505 7:+88591041 MsaT039394.1:CDS
ATGGCTTCATTCGACCAGAC+CGG 0.552260 7:-88591578 None:intergenic
TTAATTCTAACAGTCTTAGC+CGG 0.552517 7:+88591559 MsaT039394.1:CDS
TCACTGTGCTGCTTCTGGTG+GGG 0.553518 7:+88590486 MsaT039394.1:CDS
TAGTTAAGTGATTCATGGTG+TGG 0.553541 7:-88590189 None:intergenic
AGACGGGTTCTATATTGTGC+AGG 0.553860 7:-88591108 None:intergenic
CTTCCAGGAAGATGCAACGC+TGG 0.554734 7:-88591402 None:intergenic
AGCCTAAGCTTCTGCGAGCA+CGG 0.555903 7:+88591918 MsaT039394.1:CDS
GATGAGGTTGGATTATGGTA+TGG 0.556471 7:+88590310 MsaT039394.1:CDS
CGTGGAACCTATCTCTCCGA+TGG 0.558211 7:+88591845 MsaT039394.1:CDS
TGAAAGGATGTGCTGTGAGT+TGG 0.558220 7:+88590093 None:intergenic
CCCATGTCACCTTCTGGCAA+TGG 0.558647 7:+88591339 MsaT039394.1:CDS
AGGACGGGACAAATTGCTCC+AGG 0.559356 7:+88591136 MsaT039394.1:CDS
ACATGTGTGAATATGCTCAT+GGG 0.562526 7:+88591064 MsaT039394.1:CDS
AGCAGATGGTCAACGAGCAA+AGG 0.563491 7:+88590350 MsaT039394.1:CDS
AGGGGAGAATGCTCGGAGAA+GGG 0.564388 7:+88590972 MsaT039394.1:CDS
TAAGTAGCAGCAACCATCAA+AGG 0.566012 7:-88590376 None:intergenic
GACATGACAGACATCGAAGA+AGG 0.569249 7:-88591297 None:intergenic
TTCCAGGAAGATGCAACGCT+GGG 0.572420 7:-88591401 None:intergenic
GAATAAACAATAACATCTAA+CGG 0.574687 7:-88590589 None:intergenic
AGGACATTCAGTCCAATCAT+GGG 0.574757 7:-88590938 None:intergenic
CATGGCTGCAGCAAAATCCA+TGG 0.577281 7:-88591256 None:intergenic
GTATGTTTCAACTGGTTCTG+CGG 0.583036 7:+88591203 MsaT039394.1:CDS
TGAGCCAGGCAACATGCTCA+TGG 0.584222 7:-88591274 None:intergenic
GACGTGCTTGACCGAGGTGA+GGG 0.585321 7:-88591228 None:intergenic
TTCAACAGGAGATCCGGTGG+TGG 0.585729 7:-88590760 None:intergenic
CCCTTGCGAAAATCAGGACA+AGG 0.585752 7:-88591024 None:intergenic
CCGAAGCTCTTCAGGAGTGT+GGG 0.587103 7:-88591175 None:intergenic
GTGCTCTTGTCAAGGCCACA+AGG 0.587423 7:-88590457 None:intergenic
GAACCCGTCTCTGCAAGGAC+GGG 0.587749 7:+88591121 MsaT039394.1:CDS
AGAACCCGTCTCTGCAAGGA+CGG 0.587930 7:+88591120 MsaT039394.1:CDS
GCAACAACTCCTTAATGAGT+TGG 0.589263 7:+88591515 MsaT039394.1:CDS
GAAGAGCGGTGCTCTTGTCA+AGG 0.590240 7:-88590465 None:intergenic
TTATGGTATGGTCGTCGCAA+GGG 0.590907 7:+88590322 MsaT039394.1:CDS
GTTCTGCGGTTCCCTCACCT+CGG 0.594015 7:+88591217 MsaT039394.1:CDS
CGAGGAGCCTGATTTATCAT+GGG 0.594503 7:+88592088 MsaT039394.1:CDS
GACGGAGACGGAGACTGCGC+AGG 0.595411 7:-88590127 None:intergenic
GTGGAACCTATCTCTCCGAT+GGG 0.596120 7:+88591846 MsaT039394.1:CDS
TGGGAATAAGCACGTGAACA+AGG 0.597198 7:-88590919 None:intergenic
ATTATGGTATGGTCGTCGCA+AGG 0.597308 7:+88590321 MsaT039394.1:CDS
TATTAGCAGCAAGCTCAAGA+AGG 0.599518 7:-88590237 None:intergenic
ATCATGCTGTATTGGGAACA+TGG 0.599645 7:+88592237 MsaT039394.1:CDS
AACGCTGGGATGTTTGGCTG+AGG 0.600424 7:-88591387 None:intergenic
AGACGTGCTTGACCGAGGTG+AGG 0.601319 7:-88591229 None:intergenic
GCTCAAATGAAGATGATCTA+CGG 0.601438 7:-88592052 None:intergenic
CAGATCCAGCAGGAGAAAGA+GGG 0.601863 7:-88590801 None:intergenic
TTCTCAGGTGAAGCCGACAA+AGG 0.603590 7:-88590724 None:intergenic
AAGAATGTCTCCACGAAACG+TGG 0.605224 7:+88591827 MsaT039394.1:CDS
AGCCGTGCTCGCAGAAGCTT+AGG 0.607999 7:-88591920 None:intergenic
ATTGACATCCTTGAGCCCAT+CGG 0.608470 7:-88591861 None:intergenic
GCCATTGCCAGAAGGTGACA+TGG 0.611555 7:-88591340 None:intergenic
CTGTTAGAATTAATATGACG+TGG 0.612704 7:-88591549 None:intergenic
ATATAGAACCCGTCTCTGCA+AGG 0.614108 7:+88591116 MsaT039394.1:CDS
ATTCACACATGTCCCCGCGC+CGG 0.614297 7:-88591053 None:intergenic
GTTGATATCAACAGGCCTTG+TGG 0.614945 7:+88590442 MsaT039394.1:CDS
TAATTCTGTGGATGCTAACG+GGG 0.615976 7:+88590561 MsaT039394.1:CDS
GTTGGAGATAGAGACGGAGA+CGG 0.621849 7:-88590139 None:intergenic
GCTCACAATGCGGATGATGT+TGG 0.621854 7:+88592023 MsaT039394.1:CDS
ATGGGTGGAGTGAATGGTGA+AGG 0.622440 7:-88591321 None:intergenic
GATGCAGCTTGATCATCTTG+TGG 0.622855 7:+88592268 MsaT039394.1:CDS
CCAATCCAACTTACCATTGG+GGG 0.626687 7:-88592000 None:intergenic
TTGCCAGAAGGTGACATGGG+TGG 0.627061 7:-88591336 None:intergenic
ATATCGCGGAGAAGAACTCT+CGG 0.631863 7:-88591634 None:intergenic
TCAGGTGAAGCCGACAAAGG+AGG 0.632119 7:-88590721 None:intergenic
CCCAATCCAACTTACCATTG+GGG 0.638578 7:-88592001 None:intergenic
TCGCAAGGGATCGAAGCAGA+TGG 0.641118 7:+88590336 MsaT039394.1:CDS
AACTTCAACAGGAGATCCGG+TGG 0.644927 7:-88590763 None:intergenic
AGAAGCAGCACAGTGAAGAG+CGG 0.645877 7:-88590479 None:intergenic
AATAAGCACGTGAACAAGGT+CGG 0.646689 7:-88590915 None:intergenic
GTCAAAGTTGGAGATAGAGA+CGG 0.648125 7:-88590145 None:intergenic
CATGTGTGAATATGCTCATG+GGG 0.652271 7:+88591065 MsaT039394.1:CDS
AGGGGCTGATCCTAATTCTG+TGG 0.652502 7:+88590549 MsaT039394.1:CDS
GCAGAAGACGTGCTTGACCG+AGG 0.653400 7:-88591234 None:intergenic
TTTCACAAGTTCAAGCTTCG+GGG 0.656993 7:-88590611 None:intergenic
TGAGCTTGGGAACAATGGCG+AGG 0.658801 7:+88592070 MsaT039394.1:CDS
TGTGAAATGCCATTGCCAGA+AGG 0.659354 7:-88591348 None:intergenic
AGGATGTGCTGTGAGTTGGA+GGG 0.663288 7:+88590097 None:intergenic
CATATTCATTCCCTCACCCG+AGG 0.676171 7:-88592192 None:intergenic
GAACCAGTTGAAACATACAA+TGG 0.685763 7:-88591198 None:intergenic
TGAGTATGATCCTACGTCGG+TGG 0.690064 7:+88590288 MsaT039394.1:CDS
GTTGGATTGGGCTCACAATG+CGG 0.696691 7:+88592013 MsaT039394.1:CDS
AGATTTGGATCAGCATATCG+CGG 0.703328 7:-88591648 None:intergenic
CCAACCTCATCCACCGACGT+AGG 0.707714 7:-88590298 None:intergenic
CCATTGCCAGAAGGTGACAT+GGG 0.719317 7:-88591339 None:intergenic
CCTTGCGAAAATCAGGACAA+GGG 0.732087 7:-88591023 None:intergenic
GATTGAGTATGATCCTACGT+CGG 0.755912 7:+88590285 MsaT039394.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 88590100 88592304 88590100 ID=MsaG039394
Chr7 mRNA 88590100 88592304 88590100 ID=MsaT039394.1;Parent=MsaG039394
Chr7 exon 88590100 88592304 88590100 ID=MsaT039394.1.exon1;Parent=MsaT039394.1
Chr7 CDS 88590100 88592304 88590100 ID=cds.MsaT039394.1;Parent=MsaT039394.1
Gene Sequence

>MsaT039394.1

ATGTGCTGTGAGTTGGAGGGTTTAAAGCCTGCGCAGTCTCCGTCTCCGTCTCTATCTCCAACTTTGACACACAATTCTCAACCGAGAAACCACACCATGAATCACTTAACTATTGGTACTGAAGATTCTTTTGCGAGCCTTCTTGAGCTTGCTGCTAATAACGACGTTGAAGGGTTCAAACGATTGATTGAGTATGATCCTACGTCGGTGGATGAGGTTGGATTATGGTATGGTCGTCGCAAGGGATCGAAGCAGATGGTCAACGAGCAAAGGACGCCTTTGATGGTTGCTGCTACTTATGGTAGCATTGATGTTATGAAATTGATTATTTCTTTATCGGATGTTGATATCAACAGGCCTTGTGGCCTTGACAAGAGCACCGCTCTTCACTGTGCTGCTTCTGGTGGGGCTGAAAATGCTGTCGATGCTGTGAAGCTGCTTCTTGCAGCAGGGGCTGATCCTAATTCTGTGGATGCTAACGGGGATCGTCCGTTAGATGTTATTGTTTATTCCCCGAAGCTTGAACTTGTGAAAAACAGTCTTGAGGAGCTACTTCAAATTGATGGTCCTAGTGCTGGCTGTAATCTTAGGGTGATTACAAACTCGCTTAATTCTTACTCTCCTCCTTTGTCGGCTTCACCTGAGAACGGGTCTCCTTCTCCACCACCGGATCTCCTGTTGAAGTTGAAGTCAATTGATGTCCCTCTTTCTCCTGCTGGATCTGAGAAGAAAGAATATCCTGTTGATCCATCCCTTCCTGACATCAAAAACAGCATATACTCAACTGATGAATTCCGAATGTATTCTTTCAAGGTCCGACCTTGTTCACGTGCTTATTCCCATGATTGGACTGAATGTCCTTTTGTTCATCCAGGGGAGAATGCTCGGAGAAGGGACCCGAGAAAGTATCATTACAGCTGTGTCCCTTGTCCTGATTTTCGCAAGGGTGCTTGCCGGCGCGGGGACATGTGTGAATATGCTCATGGGGTTTTCGAGTGCTGGCTGCATCCTGCACAATATAGAACCCGTCTCTGCAAGGACGGGACAAATTGCTCCAGGAGAGTTTGCTTCTTTGCCCACACTCCTGAAGAGCTTCGGCCATTGTATGTTTCAACTGGTTCTGCGGTTCCCTCACCTCGGTCAAGCACGTCTTCTGCCATGGATTTTGCTGCAGCCATGAGCATGTTGCCTGGCTCACCTTCTTCGATGTCTGTCATGTCTCCTTCACCATTCACTCCACCCATGTCACCTTCTGGCAATGGCATTTCACACAACTCTGTTGCTTGGCCTCAGCCAAACATCCCAGCGTTGCATCTTCCTGGAAGTAATCTTCAGTCTAGTCGTTTGAGATCTTCTCTCAATGCTAGAGATATTCATATGGATGATTTTGAGTTGTTATCTGATTATGATCAACAGCAACAACTCCTTAATGAGTTGGCATGCCTGTCTCCACGTCATATTAATTCTAACAGTCTTAGCCGGTCTGGTCGAATGAAGCCATTGAATCCATCAAATCTTGATGATCTCTTTTCTGCCGAGAGTTCTTCTCCGCGATATGCTGATCCAAATCTGACTTCAACTGTTTTTTCTCCGACTCACAAATCAGCTGTCTTTAATCAGTTTCAGCAGCAGCAGAACATGTTGTCACCAGTGAATACAAATTTTTCTTCTAAAAATGTTGATCATCATTTATTGCAGGCAGCTTCTTATGGTGTTCAGCCATCAGGAAGAATGTCTCCACGAAACGTGGAACCTATCTCTCCGATGGGCTCAAGGATGTCAATGCTGGCACAACGCGACAAGCAGCAACAATTTCGTAGCCTAAGCTTCTGCGAGCACGGCTCTAACTCTATGCTTGCAGCTGCTGGCTCAGTCAATAATTCTTGGTCAAAATGGGAATCCCCCAATGGTAAGTTGGATTGGGCTCACAATGCGGATGATGTTGGTAAACTCCGTAGATCATCTTCATTTGAGCTTGGGAACAATGGCGAGGAGCCTGATTTATCATGGGTGCAGTCACTTGTCAAAGAATCTCCAACTGAGATCAAAGAGAAATTGGCAACTTCCATCTCAGGTGCTGCGCCAGCTGGTTCCTCGGGTGAGGGAATGAATATGAGCACACAAATGGAGTCTGTTGATCATGCTGTATTGGGAACATGGCTTGAACAGATGCAGCTTGATCATCTTGTGGCTCAGCAAAACTGA
Protein sequence

>MsaT039394.1

MCCELEGLKPAQSPSPSLSPTLTHNSQPRNHTMNHLTIGTEDSFASLLELAANNDVEGFKRLIEYDPTSVDEVGLWYGRRKGSKQMVNEQRTPLMVAATYGSIDVMKLIISLSDVDINRPCGLDKSTALHCAASGGAENAVDAVKLLLAAGADPNSVDANGDRPLDVIVYSPKLELVKNSLEELLQIDGPSAGCNLRVITNSLNSYSPPLSASPENGSPSPPPDLLLKLKSIDVPLSPAGSEKKEYPVDPSLPDIKNSIYSTDEFRMYSFKVRPCSRAYSHDWTECPFVHPGENARRRDPRKYHYSCVPCPDFRKGACRRGDMCEYAHGVFECWLHPAQYRTRLCKDGTNCSRRVCFFAHTPEELRPLYVSTGSAVPSPRSSTSSAMDFAAAMSMLPGSPSSMSVMSPSPFTPPMSPSGNGISHNSVAWPQPNIPALHLPGSNLQSSRLRSSLNARDIHMDDFELLSDYDQQQQLLNELACLSPRHINSNSLSRSGRMKPLNPSNLDDLFSAESSSPRYADPNLTSTVFSPTHKSAVFNQFQQQQNMLSPVNTNFSSKNVDHHLLQAASYGVQPSGRMSPRNVEPISPMGSRMSMLAQRDKQQQFRSLSFCEHGSNSMLAAAGSVNNSWSKWESPNGKLDWAHNADDVGKLRRSSSFELGNNGEEPDLSWVQSLVKESPTEIKEKLATSISGAAPAGSSGEGMNMSTQMESVDHAVLGTWLEQMQLDHLVAQQN*