AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG039448


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039448 MtrunA17_Chr7g0258521 85.472 530 18 3 1 530 1 471 0.0 874
MsaG039448 MtrunA17_Chr5g0438661 34.120 551 299 11 25 522 72 611 1.67e-89 287
MsaG039448 MtrunA17_Chr8g0335121 35.079 191 116 3 33 221 22 206 6.19e-23 102
MsaG039448 MtrunA17_Chr8g0335121 26.543 162 116 3 361 520 394 554 9.82e-15 77.0
MsaG039448 MtrunA17_Chr7g0249711 30.208 192 131 2 27 217 31 220 8.04e-19 89.7
MsaG039448 MtrunA17_Chr7g0249711 30.303 165 109 4 361 520 411 574 1.10e-15 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039448 AT4G19650.2 36.641 524 271 10 48 523 79 589 1.86e-110 341
MsaG039448 AT5G06810.1 35.614 570 289 13 20 523 23 580 4.27e-110 340
MsaG039448 AT5G06811.1 38.287 572 267 16 20 523 23 576 9.72e-106 328
MsaG039448 AT4G19650.1 35.887 496 257 10 76 523 90 572 1.92e-99 312
MsaG039448 AT5G45113.1 31.294 425 228 9 149 523 1 411 6.17e-61 206
MsaG039448 AT3G60400.1 25.451 554 337 18 26 520 10 546 1.51e-41 157

Find 103 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATGAGGTTTCTTGTAAT+TGG 0.139207 7:-89158326 MsaT039448.1:CDS
AGAAATGGATTGGGCGTTTA+AGG 0.166970 7:-89157775 MsaT039448.1:CDS
CTTAACTCTTGGAGATTTAT+AGG 0.169215 7:+89157548 None:intergenic
AAGGGTTCAAATTCGTTAAT+AGG 0.178407 7:+89158732 None:intergenic
TGCAGCTTGGGCTTCTTTAA+TGG 0.216279 7:+89158917 None:intergenic
TGCTGTAAGTCCAGGGATTC+TGG 0.230492 7:-89158457 MsaT039448.1:CDS
ATCCGTCTGGCCTTCAAGTT+TGG 0.231486 7:-89157407 MsaT039448.1:CDS
CAAACCCAAACTCTCGAAAA+AGG 0.236170 7:+89158713 None:intergenic
GGATGAGGTTTCTTGTAATT+GGG 0.245427 7:-89158325 MsaT039448.1:CDS
TGGTGTTCCTCGATCCAAAA+TGG 0.255150 7:-89158595 MsaT039448.1:CDS
TAAATTTGAAAATGGGGTTT+TGG 0.276379 7:-89158541 MsaT039448.1:CDS
GATTAAGGCTTATGAAGATT+TGG 0.277999 7:-89158511 MsaT039448.1:CDS
GAAATGAAGAACTGGGTTCT+TGG 0.309862 7:-89157903 MsaT039448.1:CDS
ATTAAGGCTTATGAAGATTT+GGG 0.321396 7:-89158510 MsaT039448.1:CDS
AGGTAAGGGAGCTGAGCTTC+AGG 0.325160 7:-89157745 MsaT039448.1:CDS
TTAAGCCTATGGTTGGCTTA+AGG 0.351833 7:-89157872 MsaT039448.1:CDS
ATCCTCTTGAAATGAAGAAC+TGG 0.352452 7:-89157911 MsaT039448.1:CDS
TTAGGTTGTCAATGTATAAC+TGG 0.352925 7:-89157524 MsaT039448.1:CDS
TAAGCCTATGGTTGGCTTAA+GGG 0.353016 7:-89157871 MsaT039448.1:CDS
GTTTAGAGAGGGTTTAAGAT+TGG 0.359706 7:+89159010 None:intergenic
TCCGTCTGGCCTTCAAGTTT+GGG 0.365084 7:-89157406 MsaT039448.1:CDS
ATTATCTGTGCATGCGATAA+TGG 0.370221 7:+89157458 None:intergenic
AGGGTTCAAATTCGTTAATA+GGG 0.372587 7:+89158733 None:intergenic
GCAACTCTTCCCAAACTTGA+AGG 0.394707 7:+89157397 None:intergenic
TACCACAGTCTGTGTAACTA+TGG 0.396798 7:-89158615 MsaT039448.1:CDS
ATATTCTAACAATGCAGCTT+GGG 0.419910 7:+89158905 None:intergenic
ATTGAGTATCTTGTAAAGAA+AGG 0.421238 7:-89157612 MsaT039448.1:CDS
GAGGAAAGACACTCGATGAT+TGG 0.427453 7:-89158197 MsaT039448.1:CDS
GACCACGGGGCTGTGAATCC+CGG 0.428840 7:-89157495 MsaT039448.1:CDS
GGTGTTCCTCGATCCAAAAT+GGG 0.437198 7:-89158594 MsaT039448.1:CDS
TTCTTAATCAGAACACAGAT+AGG 0.440115 7:-89157647 MsaT039448.1:CDS
TTCGCTGTGTTGTAAAATCT+AGG 0.443690 7:+89158987 None:intergenic
TATGGTTGGCTTAAGGGATC+TGG 0.446200 7:-89157865 MsaT039448.1:CDS
GATGAGGTTTCTTGTAATTG+GGG 0.448648 7:-89158324 MsaT039448.1:CDS
TGTGGAAAACACGAAAGAAA+TGG 0.448745 7:-89157790 MsaT039448.1:CDS
CTTGTTGCGCTTAGGTTATG+TGG 0.449939 7:-89157808 MsaT039448.1:CDS
TTGTGCTTGCTTTCGAATGT+TGG 0.450865 7:-89158285 MsaT039448.1:CDS
GTTTAAGGCGTTCAGAGGTA+AGG 0.457273 7:-89157760 MsaT039448.1:CDS
TGTTAATGTTGAATTTGTTA+AGG 0.462584 7:-89158427 MsaT039448.1:CDS
GTTGCTTGAATGTAGGTAAG+AGG 0.469865 7:-89157959 MsaT039448.1:intron
TCGCTGTGTTGTAAAATCTA+GGG 0.474371 7:+89158988 None:intergenic
CGAGAAACAAGAGGGCAATC+TGG 0.474907 7:+89158144 None:intergenic
TATGTAAAACGCCATCCGTC+TGG 0.479613 7:-89157420 MsaT039448.1:CDS
GACAACCTAAGCTTAACTCT+TGG 0.480332 7:+89157537 None:intergenic
AATCTCCAAGAGTTAAGCTT+AGG 0.484339 7:-89157542 MsaT039448.1:CDS
GTAAAATCTAGGGTTTAGAG+AGG 0.484384 7:+89158998 None:intergenic
AAAACACGAAAGAAATGGAT+TGG 0.486098 7:-89157785 MsaT039448.1:CDS
GATATTCTAACAATGCAGCT+TGG 0.491273 7:+89158904 None:intergenic
ACAGAGTTCTTGTTGCGCTT+AGG 0.497883 7:-89157816 MsaT039448.1:CDS
TTGATAGTGGTTGGATTCAG+TGG 0.501005 7:-89158356 MsaT039448.1:CDS
ACCACGGGGCTGTGAATCCC+GGG 0.505110 7:-89157494 MsaT039448.1:CDS
TGGAATAAGAGTTAAGCCTA+TGG 0.513478 7:-89157883 MsaT039448.1:CDS
ACCCAGTTCTTCATTTCAAG+AGG 0.514113 7:+89157909 None:intergenic
TTGAAGAATATTGTTGCAAA+GGG 0.516097 7:-89158393 MsaT039448.1:CDS
GATTTATAGGAAAGATATGA+TGG 0.516794 7:+89157561 None:intergenic
TGGTAATTCGCCATCAAAAG+AGG 0.520777 7:+89158633 None:intergenic
AATGGTGCTCAAAGCAAGCC+CGG 0.524340 7:+89157476 None:intergenic
ATTGTTGCAAAGGGTGGTGA+TGG 0.524388 7:-89158384 MsaT039448.1:CDS
AGAAATCGATTAACACGAAA+CGG 0.527963 7:-89158782 MsaT039448.1:CDS
TGGCTTAAGGGATCTGGAAG+AGG 0.530600 7:-89157859 MsaT039448.1:CDS
AAACACGAAAGAAATGGATT+GGG 0.533185 7:-89157784 MsaT039448.1:CDS
GAAGAGGAGAAATCGAGGGT+GGG 0.535364 7:-89157843 MsaT039448.1:CDS
ACATCACCAACCAGAATCCC+TGG 0.536336 7:+89158447 None:intergenic
ACTGCAAGCTTCTTGTGGAA+TGG 0.539414 7:+89158880 None:intergenic
CGAGTTTCCACAGATTTCGA+TGG 0.543091 7:-89158123 MsaT039448.1:CDS
CAGTTGGCTGAGATTATTCG+TGG 0.547737 7:-89158249 MsaT039448.1:CDS
ATAAGAGTTAAGCCTATGGT+TGG 0.547914 7:-89157879 MsaT039448.1:CDS
AGCAAAGATTCATATTCAGA+AGG 0.550210 7:+89158687 None:intergenic
AGATCCCTTAAGCCAACCAT+AGG 0.552682 7:+89157867 None:intergenic
AAGAATATTGTTGCAAAGGG+TGG 0.552691 7:-89158390 MsaT039448.1:CDS
GCGTCTATGTAGCATACTGC+AGG 0.554692 7:-89157937 MsaT039448.1:CDS
AAGAGGAGAAATCGAGGGTG+GGG 0.556838 7:-89157842 MsaT039448.1:CDS
CGTTAATAGGGTTGTAACGA+AGG 0.566099 7:+89158745 None:intergenic
GTTGAAGAATATTGTTGCAA+AGG 0.570077 7:-89158394 MsaT039448.1:CDS
AATGCTGTTGCTGTAAGTCC+AGG 0.570515 7:-89158465 MsaT039448.1:CDS
TTTAAGGCGTTCAGAGGTAA+GGG 0.573608 7:-89157759 MsaT039448.1:CDS
GGAAGAGGAGAAATCGAGGG+TGG 0.579631 7:-89157844 MsaT039448.1:CDS
ATAACTGGCTCGTAGACCAC+GGG 0.581712 7:-89157509 MsaT039448.1:CDS
TCCCAAACTTGAAGGCCAGA+CGG 0.582508 7:+89157405 None:intergenic
CTGAAGACACACCCTCAAGT+TGG 0.586419 7:+89158088 None:intergenic
ATCTGGAAGAGGAGAAATCG+AGG 0.593033 7:-89157848 MsaT039448.1:CDS
TCCTCTTGAAATGAAGAACT+GGG 0.593818 7:-89157910 MsaT039448.1:CDS
TGGGCGTTTAAGGCGTTCAG+AGG 0.599598 7:-89157765 MsaT039448.1:CDS
AAACTTGAAGGCCAGACGGA+TGG 0.607382 7:+89157409 None:intergenic
TCTTAATCAGAACACAGATA+GGG 0.611252 7:-89157646 MsaT039448.1:CDS
TAAAATCTAGGGTTTAGAGA+GGG 0.617900 7:+89158999 None:intergenic
TATAACTGGCTCGTAGACCA+CGG 0.623871 7:-89157510 MsaT039448.1:CDS
GTAAGTCCAGGGATTCTGGT+TGG 0.632171 7:-89158453 MsaT039448.1:CDS
ATGGTGCTCAAAGCAAGCCC+GGG 0.635036 7:+89157477 None:intergenic
GTGTTCTGATTAAGAATCTG+AGG 0.641643 7:+89157654 None:intergenic
GTGGAAACTCGAGAAACAAG+AGG 0.644988 7:+89158135 None:intergenic
TGGTTTCAGTGAGAAACAGT+TGG 0.649810 7:-89158265 MsaT039448.1:CDS
TGGAAACTCGAGAAACAAGA+GGG 0.652528 7:+89158136 None:intergenic
TAATAGGGTTGTAACGAAGG+TGG 0.654329 7:+89158748 None:intergenic
GTGTTCCTCGATCCAAAATG+GGG 0.658605 7:-89158593 MsaT039448.1:CDS
CACCATAGTTACACAGACTG+TGG 0.658934 7:+89158613 None:intergenic
TCAAAAGAGGATCATCGTTG+AGG 0.659008 7:+89158646 None:intergenic
TTGAGGAAAATCAAATCACG+TGG 0.663073 7:+89158663 None:intergenic
GCCCGGGATTCACAGCCCCG+TGG 0.676521 7:+89157493 None:intergenic
ATGCTGTTGCTGTAAGTCCA+GGG 0.682252 7:-89158464 MsaT039448.1:CDS
AACTTGACCATCGAAATCTG+TGG 0.684694 7:+89158116 None:intergenic
TCTGGAAGAGGAGAAATCGA+GGG 0.689277 7:-89157847 MsaT039448.1:CDS
TAACTGGCTCGTAGACCACG+GGG 0.784650 7:-89157508 MsaT039448.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 89157368 89159093 89157368 ID=MsaG039448
Chr7 mRNA 89157368 89159093 89157368 ID=MsaT039448.1;Parent=MsaG039448
Chr7 exon 89157368 89157966 89157368 ID=MsaT039448.1.exon2;Parent=MsaT039448.1
Chr7 CDS 89157368 89157966 89157368 ID=cds.MsaT039448.1;Parent=MsaT039448.1
Chr7 exon 89158100 89159093 89158100 ID=MsaT039448.1.exon1;Parent=MsaT039448.1
Chr7 CDS 89158100 89159093 89158100 ID=cds.MsaT039448.1;Parent=MsaT039448.1
Gene Sequence

>MsaT039448.1

ATGATCGACCTTAGAAAACTGAGATTAACATTCACATTTTTCAATCTCACCAAAAATCACACCAATCTTAAACCCTCTCTAAACCCTAGATTTTACAACACAGCGAAAAACCCAATTCAATTAGTTTCAAAACCTAAAATTCTCTCAAAATCAACCATTAAAGAAGCCCAAGCTGCATTGTTAGAATATCTCCATTCCACAAGAAGCTTGCAGTTTTTAGATGCAGATAACATGTGCAAAAACTCGCCTTTTTTCCTTCAAAATCTCGTCCACAAAACCCTAAAAAACGAGAAATCGATTAACACGAAACGGTTAATATCTCGCCACCTTCGTTACAACCCTATTAACGAATTTGAACCCTTTTTCGAGAGTTTGGGTTTGAAGCCTTCTGAATATGAATCTTTGCTTCCACGTGATTTGATTTTCCTCAACGATGATCCTCTTTTGATGGCGAATTACCACAGTCTGTGTAACTATGGTGTTCCTCGATCCAAAATGGGGAAAATTTTCAAACAAACGCCGCAAGTTTTTAAATTTGAAAATGGGGTTTTGGTTTCGAAGATTAAGGCTTATGAAGATTTGGGTATTTCGACTTCGATTTTAGTGAATGCTGTTGCTGTAAGTCCAGGGATTCTGGTTGGTGATGTTAATGTTGAATTTGTTAAGGTTGTTGAGATGTTGAAGAATATTGTTGCAAAGGGTGGTGATGGTGATTTTGATAGTGGTTGGATTCAGTGGCATTTTTTGGATGAGGTTTCTTGTAATTGGGGTTTAATGCTTGAGCTATTGTGCTTGCTTTCGAATGTTGGTTTCAGTGAGAAACAGTTGGCTGAGATTATTCGTGGTAGTCCTTGTATTATTTTTGAAGAATCAGGAGGAAAGACACTCGATGATTGGTTTTTTGGTCAAATTTGGATTGTCTGTGAACCAGATTGCCCTCTTGTTTCTCGAGTTTCCACAGATTTCGATGGTCAAGTTTTTTGCCAACTTGAGGGTAAGAGGCGTCTATGTAGCATACTGCAGGATAATCCTCTTGAAATGAAGAACTGGGTTCTTGGAATAAGAGTTAAGCCTATGGTTGGCTTAAGGGATCTGGAAGAGGAGAAATCGAGGGTGGGGAAGACAGAGTTCTTGTTGCGCTTAGGTTATGTGGAAAACACGAAAGAAATGGATTGGGCGTTTAAGGCGTTCAGAGGTAAGGGAGCTGAGCTTCAGGAGAGATTTGATTTTATTGTTAATACTGGATTGACACGTGATGAAGTTCGTAGAATGATTAGAGTTTCACCTCAGATTCTTAATCAGAACACAGATAGGGTCAAGATGAAAATTGAGTATCTTGTAAAGAAAGGATTTTCTGTATCAGATTTAGTGAGTTTTCCATCATATCTTTCCTATAAATCTCCAAGAGTTAAGCTTAGGTTGTCAATGTATAACTGGCTCGTAGACCACGGGGCTGTGAATCCCGGGCTTGCTTTGAGCACCATTATCGCATGCACAGATAATTTATTCTTACAAAGTTATGTAAAACGCCATCCGTCTGGCCTTCAAGTTTGGGAAGAGTTGCAAAAAGAAATTCATTCTGAAGATTCATGA

Protein sequence

>MsaT039448.1

MIDLRKLRLTFTFFNLTKNHTNLKPSLNPRFYNTAKNPIQLVSKPKILSKSTIKEAQAALLEYLHSTRSLQFLDADNMCKNSPFFLQNLVHKTLKNEKSINTKRLISRHLRYNPINEFEPFFESLGLKPSEYESLLPRDLIFLNDDPLLMANYHSLCNYGVPRSKMGKIFKQTPQVFKFENGVLVSKIKAYEDLGISTSILVNAVAVSPGILVGDVNVEFVKVVEMLKNIVAKGGDGDFDSGWIQWHFLDEVSCNWGLMLELLCLLSNVGFSEKQLAEIIRGSPCIIFEESGGKTLDDWFFGQIWIVCEPDCPLVSRVSTDFDGQVFCQLEGKRRLCSILQDNPLEMKNWVLGIRVKPMVGLRDLEEEKSRVGKTEFLLRLGYVENTKEMDWAFKAFRGKGAELQERFDFIVNTGLTRDEVRRMIRVSPQILNQNTDRVKMKIEYLVKKGFSVSDLVSFPSYLSYKSPRVKLRLSMYNWLVDHGAVNPGLALSTIIACTDNLFLQSYVKRHPSGLQVWEELQKEIHSEDS*