Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039478 | PNY05381.1 | 64.417 | 326 | 66 | 5 | 1 | 320 | 1 | 282 | 3.48e-136 | 398 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039478 | sp|Q680K8|Y1576_ARATH | 30.581 | 327 | 209 | 6 | 2 | 317 | 5 | 324 | 2.04e-46 | 162 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039478 | tr|A0A2K3NQR6|A0A2K3NQR6_TRIPR | 64.417 | 326 | 66 | 5 | 1 | 320 | 1 | 282 | 1.66e-136 | 398 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG039478 | TR | TRAF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039478 | MtrunA17_Chr4g0029841 | 82.979 | 235 | 28 | 1 | 87 | 321 | 61 | 283 | 3.31e-140 | 397 |
| MsaG039478 | MtrunA17_Chr5g0447111 | 35.474 | 327 | 187 | 11 | 2 | 314 | 7 | 323 | 8.12e-53 | 176 |
| MsaG039478 | MtrunA17_Chr3g0103031 | 29.630 | 324 | 209 | 7 | 3 | 314 | 6 | 322 | 7.81e-43 | 150 |
| MsaG039478 | MtrunA17_Chr3g0124921 | 28.443 | 334 | 214 | 8 | 1 | 319 | 1 | 324 | 9.43e-33 | 123 |
| MsaG039478 | MtrunA17_Chr5g0447191 | 41.250 | 160 | 90 | 1 | 160 | 315 | 27 | 186 | 6.30e-32 | 117 |
| MsaG039478 | MtrunA17_Chr1g0159831 | 28.529 | 333 | 207 | 10 | 5 | 317 | 6 | 327 | 4.57e-31 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039478 | AT1G55760.1 | 30.581 | 327 | 209 | 6 | 2 | 317 | 5 | 324 | 2.07e-47 | 162 |
| MsaG039478 | AT1G21780.2 | 27.829 | 327 | 205 | 9 | 5 | 314 | 6 | 318 | 5.73e-32 | 122 |
| MsaG039478 | AT1G21780.1 | 27.829 | 327 | 205 | 9 | 5 | 314 | 6 | 318 | 5.73e-32 | 122 |
Find 55 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCACCCACAATTTCATAAT+TGG | 0.099457 | 7:-89506874 | None:intergenic |
| GGAGAATCTTTCCGTGTTAA+TGG | 0.202538 | 7:+89509492 | MsaT039478.1:CDS |
| TTGAACCAAGAGCAAACTTA+TGG | 0.314969 | 7:-89509611 | None:intergenic |
| TACCTGACATGGCTAGAATT+TGG | 0.318939 | 7:-89506956 | None:intergenic |
| GAATGTCTCGGATATGTGTT+CGG | 0.366280 | 7:-89506682 | None:intergenic |
| TGATGTAATTGATCTGATAA+AGG | 0.372083 | 7:+89509803 | MsaT039478.1:CDS |
| TCTTTATCTCCTTGAATCAA+TGG | 0.385840 | 7:-89509546 | None:intergenic |
| AATGTCTCGGATATGTGTTC+GGG | 0.391475 | 7:-89506681 | None:intergenic |
| ATGGCCCATAAGTTTGCTCT+TGG | 0.405636 | 7:+89509606 | MsaT039478.1:CDS |
| CCACCCACAATTTCATAATT+GGG | 0.412916 | 7:-89506873 | None:intergenic |
| TGAACCAAGAGCAAACTTAT+GGG | 0.415322 | 7:-89509610 | None:intergenic |
| TTTCCGTAGAACTGTTGATA+TGG | 0.424881 | 7:+89509983 | MsaT039478.1:CDS |
| CTAATTTGAAAAGATGCAAT+GGG | 0.440632 | 7:-89506906 | None:intergenic |
| GCTATGACTTGGAGACTTTG+AGG | 0.456933 | 7:+89509895 | MsaT039478.1:CDS |
| CTATGACTTGGAGACTTTGA+GGG | 0.474937 | 7:+89509896 | MsaT039478.1:CDS |
| TCTGAAGATCCATTGATTCA+AGG | 0.476923 | 7:+89509537 | MsaT039478.1:CDS |
| ACCTCGATGTAGTATGCCTC+AGG | 0.503511 | 7:-89509303 | None:intergenic |
| CCTAATTTGAAAAGATGCAA+TGG | 0.504139 | 7:-89506907 | None:intergenic |
| GGATCTTCTGAATTACTGTC+AGG | 0.505900 | 7:+89509755 | MsaT039478.1:CDS |
| GGCCAAATTCTAGCCATGTC+AGG | 0.506707 | 7:+89506954 | MsaT039478.1:CDS |
| TTAAAGTCACTTGGCAGCAA+AGG | 0.527953 | 7:-89510039 | None:intergenic |
| GATAGCATACCGCTATGACT+TGG | 0.530742 | 7:+89509884 | MsaT039478.1:CDS |
| TCAAAATTGCTATGTGGCAG+TGG | 0.531985 | 7:+89506703 | MsaT039478.1:CDS |
| GCCTATCTTGTCAAATCAGT+GGG | 0.535790 | 7:+89506609 | MsaT039478.1:CDS |
| TCAGTGTGCTTGTTTGCTTG+TGG | 0.538729 | 7:+89509920 | MsaT039478.1:CDS |
| TGATAAAGGATATTGAGAAA+AGG | 0.542250 | 7:+89509817 | MsaT039478.1:CDS |
| CCCAATTATGAAATTGTGGG+TGG | 0.550348 | 7:+89506873 | MsaT039478.1:CDS |
| TATCCATATCAACAGTTCTA+CGG | 0.550571 | 7:-89509986 | None:intergenic |
| ATGTAGAAGAAGTTAGAGAC+TGG | 0.556201 | 7:+89509223 | MsaT039478.1:intron |
| ACCCACTGATTTGACAAGAT+AGG | 0.557206 | 7:-89506610 | None:intergenic |
| GTATCCCAATTATGAAATTG+TGG | 0.567040 | 7:+89506869 | MsaT039478.1:CDS |
| TATCCCAATTATGAAATTGT+GGG | 0.567156 | 7:+89506870 | MsaT039478.1:CDS |
| AATAATGATATCTCGCTCGT+GGG | 0.572526 | 7:+89506827 | MsaT039478.1:CDS |
| AAAATGTATTTAAAGTCACT+TGG | 0.574401 | 7:-89510048 | None:intergenic |
| ATCTCTTAAATCCATTAACA+CGG | 0.586157 | 7:-89509503 | None:intergenic |
| GAATAATGATATCTCGCTCG+TGG | 0.590489 | 7:+89506826 | MsaT039478.1:CDS |
| GGCCTATCTTGTCAAATCAG+TGG | 0.591536 | 7:+89506608 | MsaT039478.1:CDS |
| TATAGTTGTCATATCTGAGA+TGG | 0.593241 | 7:-89509685 | None:intergenic |
| CTACACAGAATGTAGTTGAA+AGG | 0.608683 | 7:+89509856 | MsaT039478.1:CDS |
| TATGACTTGGAGACTTTGAG+GGG | 0.609199 | 7:+89509897 | MsaT039478.1:CDS |
| TCATGATTAAATACCTGACA+TGG | 0.621425 | 7:-89506967 | None:intergenic |
| CAGTGGGTTACTTTGCTCAG+TGG | 0.630778 | 7:+89506625 | MsaT039478.1:CDS |
| GAAAGTGAATGTGAACAACT+TGG | 0.634346 | 7:+89506647 | MsaT039478.1:CDS |
| ATTTGAAAAGATGCAATGGG+GGG | 0.635780 | 7:-89506903 | None:intergenic |
| AGACATTCAAAATTGCTATG+TGG | 0.641221 | 7:+89506697 | MsaT039478.1:CDS |
| AATTTGAAAAGATGCAATGG+GGG | 0.644460 | 7:-89506904 | None:intergenic |
| TTGGACACTATCGGAAAACA+AGG | 0.646001 | 7:+89506933 | MsaT039478.1:CDS |
| CTACAATATCGTGAGAGACG+AGG | 0.649744 | 7:+89509734 | MsaT039478.1:CDS |
| TAATCTATAAATATTTGACA+TGG | 0.652304 | 7:-89509708 | None:intergenic |
| TAATTTGAAAAGATGCAATG+GGG | 0.664684 | 7:-89506905 | None:intergenic |
| ATAATGATATCTCGCTCGTG+GGG | 0.700344 | 7:+89506828 | MsaT039478.1:CDS |
| ATGTGATATTGAGCTGCCTG+AGG | 0.711463 | 7:+89509287 | MsaT039478.1:CDS |
| TTCAACTTAAGCTTAGAACG+AGG | 0.728389 | 7:+89509249 | MsaT039478.1:CDS |
| TCAAAGTCTCCAAGTCATAG+CGG | 0.744082 | 7:-89509893 | None:intergenic |
| GCCTGAGGCATACTACATCG+AGG | 0.761336 | 7:+89509302 | MsaT039478.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 89506606 | 89510055 | 89506606 | ID=MsaG039478 |
| Chr7 | mRNA | 89506606 | 89510055 | 89506606 | ID=MsaT039478.1;Parent=MsaG039478 |
| Chr7 | exon | 89506606 | 89506724 | 89506606 | ID=MsaT039478.1.exon1;Parent=MsaT039478.1 |
| Chr7 | CDS | 89506606 | 89506724 | 89506606 | ID=cds.MsaT039478.1;Parent=MsaT039478.1 |
| Chr7 | exon | 89506824 | 89506975 | 89506824 | ID=MsaT039478.1.exon2;Parent=MsaT039478.1 |
| Chr7 | CDS | 89506824 | 89506975 | 89506824 | ID=cds.MsaT039478.1;Parent=MsaT039478.1 |
| Chr7 | exon | 89509232 | 89509359 | 89509232 | ID=MsaT039478.1.exon3;Parent=MsaT039478.1 |
| Chr7 | CDS | 89509232 | 89509359 | 89509232 | ID=cds.MsaT039478.1;Parent=MsaT039478.1 |
| Chr7 | exon | 89509489 | 89510055 | 89509489 | ID=MsaT039478.1.exon4;Parent=MsaT039478.1 |
| Chr7 | CDS | 89509489 | 89510055 | 89509489 | ID=cds.MsaT039478.1;Parent=MsaT039478.1 |
| Gene Sequence |
| Protein sequence |
