Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039502 | AES81523.1 | 68.254 | 189 | 29 | 4 | 1 | 186 | 1 | 161 | 1.60e-68 | 216 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039502 | tr|G7KWB3|G7KWB3_MEDTR | 68.254 | 189 | 29 | 4 | 1 | 186 | 1 | 161 | 7.65e-69 | 216 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG039502 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG010644 | MsaG039502 | 0.806424 | 1.403645e-49 | 2.200356e-47 |
| MsaG044796 | MsaG039502 | 0.803503 | 5.690218e-49 | 8.332425e-47 |
| MsaG033850 | MsaG039502 | 0.808115 | 6.176157e-50 | 1.007875e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039502 | MtrunA17_Chr7g0258981 | 68.254 | 189 | 29 | 4 | 1 | 186 | 1 | 161 | 1.47e-72 | 216 |
| MsaG039502 | MtrunA17_Chr3g0080591 | 51.087 | 92 | 40 | 3 | 71 | 161 | 8 | 95 | 4.79e-17 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATAATATTGATGGATCTT+TGG | 0.189029 | 7:+89745954 | MsaT039502.1:CDS |
| AATAATATTGATGGATCTTT+GGG | 0.232329 | 7:+89745955 | MsaT039502.1:CDS |
| AAACTGCACAAGATCTTTCT+TGG | 0.245892 | 7:+89745674 | MsaT039502.1:CDS |
| CGACCTTGATTTGAAGCTTT+AGG | 0.290526 | 7:+89746077 | MsaT039502.1:CDS |
| ACACAATTTGCAACATAAAT+CGG | 0.316739 | 7:+89745710 | MsaT039502.1:CDS |
| CATGATTACAATAATATTGA+TGG | 0.344856 | 7:+89745946 | MsaT039502.1:CDS |
| TGTAAATCTTGTGATGTTGT+TGG | 0.389716 | 7:-89745775 | None:intergenic |
| CACAATTTGCAACATAAATC+GGG | 0.419218 | 7:+89745711 | MsaT039502.1:CDS |
| ACCGCTGGTACTTCTTCAAT+TGG | 0.421292 | 7:-89746027 | None:intergenic |
| TGTGATGAAGGTGGTGGAGA+TGG | 0.423532 | 7:-89745826 | None:intergenic |
| ACAATTGGAACCTCTGGTAT+AGG | 0.433089 | 7:-89745910 | None:intergenic |
| TGCAAATTGTGTATGAGCTT+TGG | 0.452975 | 7:-89745699 | None:intergenic |
| CAACCTAAAGCTTCAAATCA+AGG | 0.460309 | 7:-89746080 | None:intergenic |
| TGACGAGTACGAGAGACAAT+TGG | 0.479925 | 7:-89745925 | None:intergenic |
| TTGAAGAAGTACCAGCGGTC+TGG | 0.485006 | 7:+89746031 | MsaT039502.1:CDS |
| CGAGAGACAATTGGAACCTC+TGG | 0.486624 | 7:-89745916 | None:intergenic |
| TCAATTGGAGCAGAATCAAT+CGG | 0.499372 | 7:-89746012 | None:intergenic |
| ACCATGTTGTTTCCTATGCA+TGG | 0.506943 | 7:+89745850 | MsaT039502.1:CDS |
| TTTCCATCCCGCCAGACCGC+TGG | 0.512876 | 7:-89746042 | None:intergenic |
| AACCTCTGGTATAGGCCTCA+TGG | 0.524083 | 7:-89745902 | None:intergenic |
| GTACCAGCGGTCTGGCGGGA+TGG | 0.526642 | 7:+89746039 | MsaT039502.1:CDS |
| GTTGGTATGAATGTTTGTGA+TGG | 0.529752 | 7:-89745757 | None:intergenic |
| AGATCTTCTTTGTACCACAT+TGG | 0.545928 | 7:+89745799 | MsaT039502.1:CDS |
| AGAAGTACCAGCGGTCTGGC+GGG | 0.549805 | 7:+89746035 | MsaT039502.1:CDS |
| AACAACATGGTGTGTGATGA+AGG | 0.556950 | 7:-89745838 | None:intergenic |
| AAGAAGTACCAGCGGTCTGG+CGG | 0.565413 | 7:+89746034 | MsaT039502.1:CDS |
| TGGTTGCATCGAAATTCGAT+TGG | 0.568245 | 7:-89745634 | None:intergenic |
| TGGATCTTTGGGGAGAACAA+TGG | 0.571321 | 7:+89745966 | MsaT039502.1:CDS |
| TTGTTTCCTATGCATGGAAG+TGG | 0.582090 | 7:+89745856 | MsaT039502.1:CDS |
| TAGGCCTCATGGATGGATGA+AGG | 0.586352 | 7:-89745891 | None:intergenic |
| TAATTACCACTTCCATGCAT+AGG | 0.590506 | 7:-89745862 | None:intergenic |
| GTTAGAGTAAGAACAAGAGT+AGG | 0.594186 | 7:-89745610 | None:intergenic |
| GATGAAGGTGGTGGAGATGG+TGG | 0.599841 | 7:-89745823 | None:intergenic |
| TCTGGTATAGGCCTCATGGA+TGG | 0.600026 | 7:-89745898 | None:intergenic |
| GTGGAGATGGTGGTCCAATG+TGG | 0.620516 | 7:-89745813 | None:intergenic |
| ATCCATGAGGCCTATACCAG+AGG | 0.624967 | 7:+89745900 | MsaT039502.1:CDS |
| TCCAATTGAAGAAGTACCAG+CGG | 0.672889 | 7:+89746026 | MsaT039502.1:CDS |
| ATAATATTGATGGATCTTTG+GGG | 0.673989 | 7:+89745956 | MsaT039502.1:CDS |
| ATGGTGTGTGATGAAGGTGG+TGG | 0.684137 | 7:-89745832 | None:intergenic |
| GCCTCATGGATGGATGAAGG+TGG | 0.701559 | 7:-89745888 | None:intergenic |
| ACCACCTTCATCCATCCATG+AGG | 0.719012 | 7:+89745887 | MsaT039502.1:CDS |
| TCCATGCATAGGAAACAACA+TGG | 0.729158 | 7:-89745851 | None:intergenic |
| AACATGGTGTGTGATGAAGG+TGG | 0.787924 | 7:-89745835 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 89745538 | 89746098 | 89745538 | ID=MsaG039502 |
| Chr7 | mRNA | 89745538 | 89746098 | 89745538 | ID=MsaT039502.1;Parent=MsaG039502 |
| Chr7 | exon | 89745538 | 89746098 | 89745538 | ID=MsaT039502.1.exon1;Parent=MsaT039502.1 |
| Chr7 | CDS | 89745538 | 89746098 | 89745538 | ID=cds.MsaT039502.1;Parent=MsaT039502.1 |
| Gene Sequence |
| Protein sequence |
