Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039504 | AES81523.1 | 95.031 | 161 | 8 | 0 | 1 | 161 | 1 | 161 | 1.60e-100 | 296 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039504 | tr|G7KWB3|G7KWB3_MEDTR | 95.031 | 161 | 8 | 0 | 1 | 161 | 1 | 161 | 7.63e-101 | 296 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG039504 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039504 | MtrunA17_Chr7g0258981 | 95.031 | 161 | 8 | 0 | 1 | 161 | 1 | 161 | 1.47e-104 | 296 |
| MsaG039504 | MtrunA17_Chr3g0080591 | 49.451 | 91 | 22 | 2 | 72 | 138 | 32 | 122 | 2.55e-18 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATAATATCGATGGATCTT+TGG | 0.212883 | 7:+89755993 | MsaT039504.1:CDS |
| AATAATATCGATGGATCTTT+GGG | 0.246908 | 7:+89755994 | MsaT039504.1:CDS |
| GAACTGCACGAGATCTTTCT+TGG | 0.257265 | 7:+89755764 | MsaT039504.1:CDS |
| ACACAATTTGCAACATAAAT+CGG | 0.291650 | 7:+89755800 | MsaT039504.1:CDS |
| CAAATCAAGGTTGATGTCAT+TGG | 0.397831 | 7:-89756106 | None:intergenic |
| GTGCAGTTCTGAAGGTTTCA+TGG | 0.407889 | 7:-89755750 | None:intergenic |
| TGCAAATTGTGTATGTGCTT+TGG | 0.408651 | 7:-89755789 | None:intergenic |
| CACAATTTGCAACATAAATC+GGG | 0.419218 | 7:+89755801 | MsaT039504.1:CDS |
| ACAATTGGAACCTCTGGTAT+AGG | 0.433089 | 7:-89755949 | None:intergenic |
| TGGCAATGGTTGCATGGAAT+AGG | 0.448209 | 7:-89755730 | None:intergenic |
| TTCTGAAGGTTTCATGGCAA+TGG | 0.457643 | 7:-89755744 | None:intergenic |
| TCAGATGATGAGAGCAGTAC+TGG | 0.465223 | 7:+89755682 | MsaT039504.1:CDS |
| CGATCAGTACGAGAGACAAT+TGG | 0.466761 | 7:-89755964 | None:intergenic |
| TCAGTTGGAGCTGAATCAAT+CGG | 0.471657 | 7:-89756051 | None:intergenic |
| CAACCTAGAGCTTCAAATCA+AGG | 0.475468 | 7:-89756119 | None:intergenic |
| CGAGAGACAATTGGAACCTC+TGG | 0.486624 | 7:-89755955 | None:intergenic |
| TCTCATCATCTGATTCAGAT+AGG | 0.499444 | 7:-89755672 | None:intergenic |
| CTTCCATCCCGCCACACCAC+TGG | 0.504811 | 7:-89756081 | None:intergenic |
| TCTGGTATAGGCCTCATGTA+TGG | 0.510085 | 7:-89755937 | None:intergenic |
| ACCACTGGTAATTCTTCAGT+TGG | 0.510267 | 7:-89756066 | None:intergenic |
| CAACCTTGATTTGAAGCTCT+AGG | 0.512638 | 7:+89756116 | MsaT039504.1:CDS |
| TGTGATGAAGGTGGTGGAGA+TGG | 0.521845 | 7:-89755862 | None:intergenic |
| TAATTACCGCTTCCATGCAT+AGG | 0.528205 | 7:-89755898 | None:intergenic |
| ACTATGTTGTTTCCTATGCA+TGG | 0.528781 | 7:+89755886 | MsaT039504.1:CDS |
| TTACCAGTGGTGTGGCGGGA+TGG | 0.529438 | 7:+89756078 | MsaT039504.1:CDS |
| CGTGATTACAATAATATCGA+TGG | 0.538614 | 7:+89755985 | MsaT039504.1:CDS |
| GTTTCATGGCAATGGTTGCA+TGG | 0.544580 | 7:-89755736 | None:intergenic |
| AAGAATTACCAGTGGTGTGG+CGG | 0.548905 | 7:+89756073 | MsaT039504.1:CDS |
| GCCTCATGTATGGATGAAAA+CGG | 0.550640 | 7:-89755927 | None:intergenic |
| AAGATCTCGTGCAGTTCTGA+AGG | 0.551614 | 7:-89755758 | None:intergenic |
| TTGTTTCCTATGCATGGAAG+CGG | 0.563807 | 7:+89755892 | MsaT039504.1:CDS |
| TGGATCTTTGGGGAGAACAA+TGG | 0.585871 | 7:+89756005 | MsaT039504.1:CDS |
| AACAACATAGTGTGTGATGA+AGG | 0.595436 | 7:-89755874 | None:intergenic |
| AGAATTACCAGTGGTGTGGC+GGG | 0.597554 | 7:+89756074 | MsaT039504.1:CDS |
| GATGAAGGTGGTGGAGATGG+TGG | 0.599841 | 7:-89755859 | None:intergenic |
| GTGGAGATGGTGGTCCAATG+TGG | 0.619599 | 7:-89755849 | None:intergenic |
| CTAAACAACACACACCACAT+TGG | 0.630539 | 7:+89755835 | MsaT039504.1:CDS |
| CTGAAGAATTACCAGTGGTG+TGG | 0.651724 | 7:+89756070 | MsaT039504.1:CDS |
| ATACATGAGGCCTATACCAG+AGG | 0.686494 | 7:+89755939 | MsaT039504.1:CDS |
| TCCAACTGAAGAATTACCAG+TGG | 0.699300 | 7:+89756065 | MsaT039504.1:CDS |
| ATAGTGTGTGATGAAGGTGG+TGG | 0.699659 | 7:-89755868 | None:intergenic |
| ATAATATCGATGGATCTTTG+GGG | 0.708326 | 7:+89755995 | MsaT039504.1:CDS |
| AACATAGTGTGTGATGAAGG+TGG | 0.727614 | 7:-89755871 | None:intergenic |
| TCATGTATGGATGAAAACGG+CGG | 0.744847 | 7:-89755924 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 89755652 | 89756137 | 89755652 | ID=MsaG039504 |
| Chr7 | mRNA | 89755652 | 89756137 | 89755652 | ID=MsaT039504.1;Parent=MsaG039504 |
| Chr7 | exon | 89755652 | 89756137 | 89755652 | ID=MsaT039504.1.exon1;Parent=MsaT039504.1 |
| Chr7 | CDS | 89755652 | 89756137 | 89755652 | ID=cds.MsaT039504.1;Parent=MsaT039504.1 |
| Gene Sequence |
| Protein sequence |
