AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG039622


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039622 MtrunA17_Chr7g0260331 96.894 322 8 1 1 320 1 322 0.0 643
MsaG039622 MtrunA17_Chr1g0185131 57.396 338 100 7 1 325 1 307 4.82e-109 325
MsaG039622 MtrunA17_Chr1g0204741 56.711 298 115 5 26 321 17 302 1.26e-106 318
MsaG039622 MtrunA17_Chr3g0135831 63.761 218 73 2 34 251 31 242 3.76e-95 290
MsaG039622 MtrunA17_Chr5g0410881 58.559 222 86 1 35 250 18 239 1.91e-87 275
MsaG039622 MtrunA17_Chr4g0039891 57.005 207 84 1 48 249 11 217 6.87e-77 247
MsaG039622 MtrunA17_Chr4g0071051 45.172 290 130 5 7 289 67 334 3.48e-73 235
MsaG039622 MtrunA17_Chr7g0260361 96.040 101 4 0 321 421 59 159 1.52e-62 199
MsaG039622 MtrunA17_Chr8g0379701 41.667 228 121 3 48 275 11 226 1.12e-52 181
MsaG039622 MtrunA17_Chr8g0392001 47.087 206 95 3 49 244 22 223 1.52e-52 180
MsaG039622 MtrunA17_Chr6g0485571 43.415 205 107 3 49 253 11 206 6.37e-52 177
MsaG039622 MtrunA17_Chr1g0204631 77.228 101 23 0 321 421 113 213 1.29e-49 167
MsaG039622 MtrunA17_Chr6g0467041 44.878 205 99 5 49 250 22 215 4.97e-49 169
MsaG039622 MtrunA17_Chr8g0378061 47.059 204 98 6 50 252 23 217 8.93e-49 172
MsaG039622 MtrunA17_Chr2g0284481 36.800 250 131 5 48 284 14 249 4.31e-46 163
MsaG039622 MtrunA17_Chr2g0330821 43.386 189 102 2 43 231 73 256 2.49e-45 160
MsaG039622 MtrunA17_Chr5g0402291 66.667 102 34 0 46 147 6 107 4.47e-44 155
MsaG039622 MtrunA17_Chr3g0134031 68.932 103 31 1 43 145 18 119 1.75e-43 156
MsaG039622 MtrunA17_Chr5g0397881 65.957 94 32 0 47 140 22 115 6.81e-42 151
MsaG039622 MtrunA17_Chr1g0203601 61.702 94 36 0 47 140 16 109 5.42e-40 144
MsaG039622 MtrunA17_Chr5g0441531 65.957 94 32 0 47 140 23 116 1.92e-39 142
MsaG039622 MtrunA17_Chr7g0257331 45.062 162 77 3 40 189 9 170 2.90e-39 140
MsaG039622 MtrunA17_Chr6g0472421 60.638 94 37 0 47 140 31 124 1.94e-38 141
MsaG039622 MtrunA17_Chr8g0375041 43.605 172 78 3 44 199 4 172 1.92e-37 136
MsaG039622 MtrunA17_Chr4g0047421 38.462 182 97 5 47 224 31 201 1.53e-32 122
MsaG039622 MtrunA17_Chr1g0179321 66.216 74 25 0 35 108 35 108 1.79e-30 113
MsaG039622 MtrunA17_Chr1g0179311 44.578 166 85 5 106 270 11 170 1.01e-29 118
MsaG039622 MtrunA17_Chr4g0023771 57.143 70 29 1 40 108 6 75 3.57e-21 88.2
MsaG039622 MtrunA17_Chr2g0330841 32.544 169 103 5 109 273 41 202 1.20e-18 87.4
MsaG039622 MtrunA17_Chr5g0448141 39.604 101 61 0 321 421 114 214 3.60e-15 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039622 AT3G22830.1 54.722 360 119 7 22 338 34 392 3.88e-122 360
MsaG039622 AT3G51910.1 54.514 288 92 6 39 325 19 268 2.65e-96 290
MsaG039622 AT2G26150.1 61.111 216 82 2 36 250 31 245 3.02e-90 277
MsaG039622 AT2G26150.4 61.111 216 82 2 36 250 31 245 3.02e-90 277
MsaG039622 AT3G63350.1 47.297 296 119 4 27 320 6 266 1.63e-83 257
MsaG039622 AT1G32330.1 58.333 228 80 4 36 250 24 249 4.47e-82 260
MsaG039622 AT1G32330.2 58.333 228 80 4 36 250 24 249 4.50e-82 260
MsaG039622 AT4G17750.1 48.551 276 115 5 26 280 23 292 5.89e-82 260
MsaG039622 AT5G16820.1 51.538 260 110 5 27 276 10 263 3.42e-79 253
MsaG039622 AT5G16820.2 51.538 260 110 5 27 276 10 263 3.42e-79 253
MsaG039622 AT3G02990.1 53.913 230 99 3 48 270 22 251 1.92e-77 248
MsaG039622 AT5G43840.1 45.652 276 118 6 35 297 5 261 6.97e-74 233
MsaG039622 AT5G03720.2 46.296 270 123 6 31 288 37 296 4.75e-68 222
MsaG039622 AT5G03720.1 46.296 270 123 6 31 288 37 296 4.75e-68 222
MsaG039622 AT1G67970.1 51.053 190 91 2 49 237 19 207 1.25e-59 199
MsaG039622 AT5G54070.1 41.262 206 108 3 42 244 63 258 4.27e-53 180
MsaG039622 AT3G22845.1 79.208 101 21 0 321 421 114 214 1.78e-51 172
MsaG039622 AT3G24520.1 42.857 203 92 2 49 251 17 195 3.22e-50 173
MsaG039622 AT4G13980.1 43.478 207 102 4 50 252 24 219 1.56e-48 172
MsaG039622 AT2G26150.3 55.245 143 62 2 109 250 49 190 1.39e-44 157
MsaG039622 AT2G26150.2 55.245 143 62 2 109 250 49 190 1.39e-44 157
MsaG039622 AT4G18880.1 43.455 191 108 0 41 231 7 197 1.22e-43 157
MsaG039622 AT1G46264.1 66.981 106 34 1 39 144 24 128 1.47e-42 153
MsaG039622 AT5G45710.3 41.146 192 111 2 48 239 12 201 1.47e-41 150
MsaG039622 AT5G45710.1 41.146 192 111 2 48 239 12 201 1.47e-41 150
MsaG039622 AT4G11660.1 63.636 99 35 1 47 145 57 154 1.01e-39 146
MsaG039622 AT5G62020.1 50.769 130 60 1 47 172 21 150 2.15e-39 143
MsaG039622 AT4G36990.1 56.522 115 43 1 47 161 12 119 3.32e-39 142
MsaG039622 AT2G41690.1 62.766 94 35 0 47 140 38 131 1.03e-38 140
MsaG039622 AT4G18870.1 36.290 124 79 0 38 161 135 258 4.01e-24 101
MsaG039622 AT4G18870.1 49.438 89 40 2 50 136 14 99 1.05e-21 95.1
MsaG039622 AT3G07680.1 39.604 101 61 0 321 421 108 208 1.44e-14 72.8

Find 81 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCAATCTTCACATTAAT+TGG 0.154501 7:-91154306 None:intergenic
AAGAACTTGATGAAGGGTTT+TGG 0.252613 7:+91147430 MsaT039622.1:CDS
TTTGCAAACTCCCATTTATC+AGG 0.290173 7:-91146847 None:intergenic
TGGTGCTAGCTTTGTTGTTT+GGG 0.306922 7:+91145959 MsaT039622.1:CDS
GTGGTGCTAGCTTTGTTGTT+TGG 0.312326 7:+91145958 MsaT039622.1:CDS
TTAGCTAAGTTATGCACATT+TGG 0.329783 7:-91152037 None:intergenic
TGTGTTGAAGTTGGTCGTTT+TGG 0.363566 7:+91146973 MsaT039622.1:CDS
CATTTGTTGCTGCTTAATTT+CGG 0.365368 7:-91147124 None:intergenic
AGGAGAAGGCCAATTGAGTA+TGG 0.367381 7:+91147249 MsaT039622.1:CDS
CTTGATTCTCCATACTCAAT+TGG 0.368141 7:-91147258 None:intergenic
TCTAAATACTCTTCTTTCAC+TGG 0.377680 7:-91145759 None:intergenic
TTCCTCCATTGCTTGAAGAA+TGG 0.378113 7:-91147088 None:intergenic
CAACAAGTCACATAGTTTCA+TGG 0.398902 7:+91145928 MsaT039622.1:CDS
CACAAAGACATTTGATGTTG+TGG 0.406218 7:+91145896 MsaT039622.1:CDS
AAATGGGAGTTTGCAAATGA+AGG 0.436132 7:+91146853 MsaT039622.1:CDS
GCTATGGAAATGCAGGGTCT+TGG 0.436909 7:+91147345 MsaT039622.1:CDS
TCAAATGTCTTTGTGAGAAA+TGG 0.443148 7:-91145888 None:intergenic
AGGTCTCTGGAGAAAGCATT+TGG 0.449261 7:-91145984 None:intergenic
TGCTGAGTTAAGAGAAGCAT+TGG 0.450097 7:+91154323 MsaT039622.1:CDS
TTGTTGTGTTTGAAGTATCT+AGG 0.454609 7:-91146011 None:intergenic
GGAAGGAGCAGTGTTAGATA+TGG 0.456890 7:+91147294 MsaT039622.1:CDS
ATGAATAAAGAACTTGATGA+AGG 0.466508 7:+91147423 MsaT039622.1:CDS
AAGAGGTGGTGGAGAGCTTG+AGG 0.469597 7:+91147368 MsaT039622.1:CDS
GTATCTAGGAAGAAGGTCTC+TGG 0.470929 7:-91145997 None:intergenic
AGAGCTTGAGGAAGAGATTG+AGG 0.477721 7:+91147380 MsaT039622.1:CDS
AGTAATTGCTGAATAAAAGC+TGG 0.482397 7:-91147177 None:intergenic
CAAGGTCATTGTGTTGAAGT+TGG 0.497401 7:+91146964 MsaT039622.1:CDS
GTGGCCAACATGAACATAGA+AGG 0.501930 7:-91152011 None:intergenic
GTTATTCCACGGCCAATGGA+GGG 0.502097 7:+91145843 MsaT039622.1:CDS
GTGCATAACTTAGCTAAAGA+TGG 0.502933 7:+91152043 MsaT039622.1:CDS
CAGGGTCTTGGAAGAGGTGG+TGG 0.504100 7:+91147357 MsaT039622.1:CDS
GGTTGTTATTCCACGGCCAA+TGG 0.506460 7:+91145839 MsaT039622.1:CDS
TGGATTTGAAGTGTCAGAGT+TGG 0.506828 7:+91147314 MsaT039622.1:CDS
GATTGTTGTTGTGAAGTACT+AGG 0.510219 7:-91146937 None:intergenic
AATTAAGCAGCAACAAATGA+TGG 0.512562 7:+91147128 MsaT039622.1:CDS
GCAAATGAAGGATTTCTAAG+AGG 0.518927 7:+91146865 MsaT039622.1:CDS
TGTTATTCCACGGCCAATGG+AGG 0.521139 7:+91145842 MsaT039622.1:CDS
ACTTCATGAAGCCCCTCCAT+TGG 0.523280 7:-91145855 None:intergenic
GTTTGAAGTATCTAGGAAGA+AGG 0.525332 7:-91146004 None:intergenic
TGAAGCCCCTCCATTGGCCG+TGG 0.526845 7:-91145849 None:intergenic
TCAGCAATTACTTCAACAGA+AGG 0.528664 7:+91147188 MsaT039622.1:CDS
TGAAACTATGTGACTTGTTG+TGG 0.534841 7:-91145925 None:intergenic
AATGCTGGCTATGGAAATGC+AGG 0.541022 7:+91147338 MsaT039622.1:CDS
GAATCAAGTAATGGTGGTGA+TGG 0.544567 7:+91147273 MsaT039622.1:CDS
CTAAATACTCTTCTTTCACT+GGG 0.546539 7:-91145758 None:intergenic
GAAATGCAGGGTCTTGGAAG+AGG 0.548123 7:+91147351 MsaT039622.1:CDS
TGACAAACAAGTGTTAATGA+TGG 0.548560 7:+91147026 MsaT039622.1:CDS
TCAACAGAAGGAAAAGAGAA+AGG 0.548892 7:+91147200 MsaT039622.1:CDS
TATGAAAGAGGAAGTGATGA+GGG 0.553152 7:+91145810 MsaT039622.1:CDS
TTATTCCACGGCCAATGGAG+GGG 0.553219 7:+91145844 MsaT039622.1:CDS
GAAACTATGTGACTTGTTGT+GGG 0.554070 7:-91145924 None:intergenic
TTCTTCAAGCAATGGAGGAA+AGG 0.554568 7:+91147091 MsaT039622.1:CDS
AGAGTTGGAAATGCTGGCTA+TGG 0.563110 7:+91147329 MsaT039622.1:CDS
GATCAACGGTTGTTATTCCA+CGG 0.563873 7:+91145832 MsaT039622.1:CDS
ATGGAGGGGCTTCATGAAGT+AGG 0.569714 7:+91145858 MsaT039622.1:CDS
CAAGTAATGGTGGTGATGGA+AGG 0.570233 7:+91147277 MsaT039622.1:CDS
AGTGTCAGAGTTGGAAATGC+TGG 0.571819 7:+91147323 MsaT039622.1:CDS
TGAATAAAGAACTTGATGAA+GGG 0.573949 7:+91147424 MsaT039622.1:CDS
ATCCATTCTTCAAGCAATGG+AGG 0.576630 7:+91147086 MsaT039622.1:CDS
TCATCATCGATATATGAAAG+AGG 0.577051 7:+91145798 MsaT039622.1:CDS
AAGATGCTATTACTAAGAAG+AGG 0.582617 7:+91147229 MsaT039622.1:CDS
ATAGTTTCATGGAGCAGAAG+TGG 0.585048 7:+91145939 MsaT039622.1:CDS
AAGATCCATTCTTCAAGCAA+TGG 0.586126 7:+91147083 MsaT039622.1:CDS
GTGTCCTTCTATGTTCATGT+TGG 0.591878 7:+91152007 MsaT039622.1:CDS
AGTTATGCACATTTGGAATG+TGG 0.602220 7:-91152030 None:intergenic
GAGTACAAGCTTATTGCAAG+TGG 0.604992 7:+91154590 MsaT039622.1:CDS
AGGAAGTGATGAGGGATCAA+CGG 0.610055 7:+91145818 MsaT039622.1:CDS
GAGTATGGAGAATCAAGTAA+TGG 0.613238 7:+91147264 MsaT039622.1:CDS
ATGCAGGGTCTTGGAAGAGG+TGG 0.614702 7:+91147354 MsaT039622.1:CDS
ATATGAAAGAGGAAGTGATG+AGG 0.618653 7:+91145809 MsaT039622.1:CDS
CTATTACTAAGAAGAGGAGA+AGG 0.622937 7:+91147235 MsaT039622.1:CDS
AAAGACATTTGATGTTGTGG+AGG 0.626606 7:+91145899 MsaT039622.1:CDS
AATGTCTTTGTGAGAAATGG+TGG 0.633018 7:-91145885 None:intergenic
TTCATATATCGATGATGACG+AGG 0.649679 7:-91145793 None:intergenic
TATGGAGAATCAAGTAATGG+TGG 0.653267 7:+91147267 MsaT039622.1:CDS
GTCTTTGTGAGAAATGGTGG+TGG 0.661352 7:-91145882 None:intergenic
TCACAACAACAATCATCACA+AGG 0.699849 7:+91146946 MsaT039622.1:CDS
GCAATGGAGGAAAGGCTACG+TGG 0.702933 7:+91147099 MsaT039622.1:CDS
AAACTTGTAAGGTCTACACT+CGG 0.707311 7:-91154653 None:intergenic
AAACTATGTGACTTGTTGTG+GGG 0.712916 7:-91145923 None:intergenic
ATGCTGGCTATGGAAATGCA+GGG 0.739262 7:+91147339 MsaT039622.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 91145744 91154662 91145744 ID=MsaG039622
Chr7 mRNA 91145744 91154662 91145744 ID=MsaT039622.1;Parent=MsaG039622
Chr7 exon 91145744 91146067 91145744 ID=MsaT039622.1.exon1;Parent=MsaT039622.1
Chr7 CDS 91145744 91146067 91145744 ID=cds.MsaT039622.1;Parent=MsaT039622.1
Chr7 exon 91146829 91147465 91146829 ID=MsaT039622.1.exon2;Parent=MsaT039622.1
Chr7 CDS 91146829 91147465 91146829 ID=cds.MsaT039622.1;Parent=MsaT039622.1
Chr7 exon 91152002 91152064 91152002 ID=MsaT039622.1.exon3;Parent=MsaT039622.1
Chr7 CDS 91152002 91152064 91152002 ID=cds.MsaT039622.1;Parent=MsaT039622.1
Chr7 exon 91154295 91154402 91154295 ID=MsaT039622.1.exon4;Parent=MsaT039622.1
Chr7 CDS 91154295 91154402 91154295 ID=cds.MsaT039622.1;Parent=MsaT039622.1
Chr7 exon 91154529 91154662 91154529 ID=MsaT039622.1.exon5;Parent=MsaT039622.1
Chr7 CDS 91154529 91154662 91154529 ID=cds.MsaT039622.1;Parent=MsaT039622.1
Gene Sequence

>MsaT039622.1

ATGAACTATCTCTACCCAGTGAAAGAAGAGTATTTAGAAATTGAAGCAACCTCGTCATCATCGATATATGAAAGAGGAAGTGATGAGGGATCAACGGTTGTTATTCCACGGCCAATGGAGGGGCTTCATGAAGTAGGACCACCACCATTTCTCACAAAGACATTTGATGTTGTGGAGGACCCCACAACAAGTCACATAGTTTCATGGAGCAGAAGTGGTGCTAGCTTTGTTGTTTGGGATCCAAATGCTTTCTCCAGAGACCTTCTTCCTAGATACTTCAAACACAACAATTTCTCAAGTTTTGTTAGACAACTCAACACCTATGGTTTTAGAAAAATTGATCCTGATAAATGGGAGTTTGCAAATGAAGGATTTCTAAGAGGACATAAACATCTTTTAGCAAACATTAAGAGAAGGAGACAAACTTCACAACCTAGTACTTCACAACAACAATCATCACAAGGTCATTGTGTTGAAGTTGGTCGTTTTGGATTAGACGAAGAAATCGATCATTTGAGACGTGACAAACAAGTGTTAATGATGGAACTTGTGAAGCTTAGACAGCAACAGCAGAACACAAGATCCATTCTTCAAGCAATGGAGGAAAGGCTACGTGGAACCGAAATTAAGCAGCAACAAATGATGGCTTTTTTGGCTAGAGCTATGAAAAATCCAGCTTTTATTCAGCAATTACTTCAACAGAAGGAAAAGAGAAAGGAGATTGAAGATGCTATTACTAAGAAGAGGAGAAGGCCAATTGAGTATGGAGAATCAAGTAATGGTGGTGATGGAAGGAGCAGTGTTAGATATGGATTTGAAGTGTCAGAGTTGGAAATGCTGGCTATGGAAATGCAGGGTCTTGGAAGAGGTGGTGGAGAGCTTGAGGAAGAGATTGAGGTATTTGAGACTCATGAGAAAATGAATAAAGAACTTGATGAAGGGTTTTGGGAAGAACTTTTTAAAACTGTGTCCTTCTATGTTCATGTTGGCCACATTCCAAATGTGCATAACTTAGCTAAAGATGAGCATTTTGATCCAATTAATGTGAAGATTGCTGAGTTAAGAGAAGCATTGGAATCTATTATTACAGAACAAAAGTACTTGAAAGCACGTGATACTAGACATCGACATACAAACGAGAGCACCAGAAAACGTGTTGTCTTTTACACACTCTTGGAGTATATTTTATTTGTGAGTACAAGCTTATTGCAAGTGGTATACATTCGTCGTCTCTTCAGCAAATCATTTGCTTACAACCGAGTGTAG

Protein sequence

>MsaT039622.1

MNYLYPVKEEYLEIEATSSSSIYERGSDEGSTVVIPRPMEGLHEVGPPPFLTKTFDVVEDPTTSHIVSWSRSGASFVVWDPNAFSRDLLPRYFKHNNFSSFVRQLNTYGFRKIDPDKWEFANEGFLRGHKHLLANIKRRRQTSQPSTSQQQSSQGHCVEVGRFGLDEEIDHLRRDKQVLMMELVKLRQQQQNTRSILQAMEERLRGTEIKQQQMMAFLARAMKNPAFIQQLLQQKEKRKEIEDAITKKRRRPIEYGESSNGGDGRSSVRYGFEVSELEMLAMEMQGLGRGGGELEEEIEVFETHEKMNKELDEGFWEELFKTVSFYVHVGHIPNVHNLAKDEHFDPINVKIAELREALESIITEQKYLKARDTRHRHTNESTRKRVVFYTLLEYILFVSTSLLQVVYIRRLFSKSFAYNRV*