AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG039686

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039686 MtrunA17_Chr7g0260971 98.165 218 4 0 1 218 1 218 5.75e-164 451
MsaG039686 MtrunA17_Chr1g0184281 59.649 228 59 10 1 218 1 205 3.82e-79 236
MsaG039686 MtrunA17_Chr7g0261031 70.796 113 33 0 9 121 3 115 4.74e-55 175
MsaG039686 MtrunA17_Chr1g0184271 71.429 105 30 0 14 118 12 116 1.94e-52 169
MsaG039686 MtrunA17_Chr8g0351721 67.857 112 36 0 5 116 9 120 8.65e-51 164
MsaG039686 MtrunA17_Chr8g0351701 69.903 103 31 0 14 116 23 125 7.49e-50 162
MsaG039686 MtrunA17_Chr6g0460951 67.961 103 33 0 13 115 39 141 8.86e-47 154
MsaG039686 MtrunA17_Chr5g0435641 64.000 100 36 0 9 108 2 101 1.45e-45 150
MsaG039686 MtrunA17_Chr7g0245871 66.667 102 34 0 14 115 27 128 4.62e-45 150
MsaG039686 MtrunA17_Chr2g0311601 57.143 112 47 1 6 117 5 115 2.45e-43 143
MsaG039686 MtrunA17_Chr4g0043421 47.500 160 74 5 1 159 1 151 3.96e-41 138
MsaG039686 MtrunA17_Chr7g0227511 60.000 115 42 2 1 115 1 111 1.53e-40 136
MsaG039686 MtrunA17_Chr7g0245861 68.367 98 29 2 6 101 4 101 4.24e-40 134
MsaG039686 MtrunA17_Chr5g0437571 56.604 106 45 1 10 115 2 106 1.04e-38 131
MsaG039686 MtrunA17_Chr3g0117121 57.273 110 46 1 6 115 2 110 2.09e-38 131
MsaG039686 MtrunA17_Chr3g0114061 51.613 124 58 2 11 133 5 127 4.98e-38 129
MsaG039686 MtrunA17_Chr4g0058651 63.542 96 34 1 13 108 10 104 1.11e-37 129
MsaG039686 MtrunA17_Chr3g0106101 56.075 107 45 2 10 115 2 107 1.50e-37 129
MsaG039686 MtrunA17_Chr6g0449641 56.881 109 46 1 7 115 2 109 1.99e-37 127
MsaG039686 MtrunA17_Chr5g0438091 53.333 105 48 1 11 115 36 139 1.92e-36 126
MsaG039686 MtrunA17_Chr5g0402611 55.357 112 49 1 8 119 2 112 4.54e-36 129
MsaG039686 MtrunA17_Chr5g0437401 51.327 113 54 1 5 117 2 113 7.79e-36 124
MsaG039686 MtrunA17_Chr6g0449651 57.426 101 42 1 8 108 61 160 1.44e-34 122
MsaG039686 MtrunA17_Chr3g0097221 54.630 108 48 1 9 116 2 108 2.12e-34 124
MsaG039686 MtrunA17_Chr3g0129071 53.333 105 48 1 11 115 44 147 4.52e-34 121
MsaG039686 MtrunA17_Chr3g0113891 50.000 112 55 1 1 112 1 111 6.15e-34 120
MsaG039686 MtrunA17_Chr5g0432451 56.250 112 48 1 4 115 33 143 7.37e-34 120
MsaG039686 MtrunA17_Chr3g0115431 55.046 109 48 1 8 116 3 110 1.52e-33 120
MsaG039686 MtrunA17_Chr4g0030001 50.000 114 55 2 3 115 13 125 1.91e-33 119
MsaG039686 MtrunA17_Chr8g0353981 56.897 116 42 2 1 116 1 108 4.86e-33 119
MsaG039686 MtrunA17_Chr6g0460941 63.636 88 32 0 14 101 7 94 1.12e-32 114
MsaG039686 MtrunA17_Chr7g0225111 43.363 113 63 1 13 125 4 115 3.78e-30 108
MsaG039686 MtrunA17_Chr5g0444351 42.742 124 69 2 15 137 6 128 1.21e-29 107
MsaG039686 MtrunA17_Chr1g0200201 50.495 101 49 1 15 115 73 172 2.29e-29 109
MsaG039686 MtrunA17_Chr4g0059901 41.509 106 61 1 10 115 6 110 1.55e-23 95.1
MsaG039686 MtrunA17_Chr8g0374601 43.689 103 57 1 14 116 26 127 2.73e-23 94.7
MsaG039686 MtrunA17_Chr8g0374591 41.748 103 59 1 14 116 8 109 1.32e-21 86.3
MsaG039686 MtrunA17_Chr8g0342351 40.566 106 62 1 11 116 9 113 9.06e-21 87.4
MsaG039686 MtrunA17_Chr2g0330201 37.383 107 66 1 10 116 15 120 8.40e-20 85.5
MsaG039686 MtrunA17_Chr6g0452671 32.824 131 87 1 11 141 8 137 2.15e-19 82.4
MsaG039686 MtrunA17_Chr6g0452691 30.645 186 117 6 15 199 10 184 1.31e-18 80.1
MsaG039686 MtrunA17_Chr6g0452661 35.780 109 68 2 15 122 10 117 1.61e-17 78.6
MsaG039686 MtrunA17_Chr8g0374581 39.806 103 60 2 14 115 8 109 3.36e-17 77.4
MsaG039686 MtrunA17_Chr4g0040831 34.188 117 66 2 8 114 9 124 2.30e-16 74.3
MsaG039686 MtrunA17_Chr4g0055441 35.780 109 68 2 15 122 3 110 5.78e-16 72.4
MsaG039686 MtrunA17_Chr2g0326041 56.250 48 20 1 13 60 32 78 1.77e-13 63.9
MsaG039686 MtrunA17_Chr5g0439111 33.654 104 67 2 15 117 10 112 4.88e-13 65.9
MsaG039686 MtrunA17_Chr2g0326051 50.000 54 27 0 62 115 7 60 3.46e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039686 AT2G42430.1 55.422 249 76 9 1 218 1 245 1.67e-79 238
MsaG039686 AT3G58190.1 62.205 127 40 1 14 132 11 137 1.69e-53 171
MsaG039686 AT3G03760.1 76.699 103 24 0 13 115 50 152 1.13e-51 168
MsaG039686 AT4G00210.1 66.964 112 37 0 5 116 2 113 2.08e-51 166
MsaG039686 AT2G45420.1 76.000 100 24 0 17 116 40 139 1.91e-50 165
MsaG039686 AT2G42440.1 61.600 125 40 1 9 125 2 126 2.01e-50 164
MsaG039686 AT2G31310.1 61.404 114 44 0 10 123 3 116 3.30e-50 162
MsaG039686 AT5G06080.1 60.331 121 47 1 12 132 5 124 1.61e-47 154
MsaG039686 AT4G00220.1 69.000 100 31 0 17 116 20 119 1.34e-45 151
MsaG039686 AT2G45410.1 68.000 100 32 0 17 116 19 118 8.40e-45 148
MsaG039686 AT5G63090.4 58.120 117 45 2 1 117 1 113 3.72e-39 133
MsaG039686 AT5G63090.3 58.120 117 45 2 1 117 1 113 3.72e-39 133
MsaG039686 AT5G63090.2 58.120 117 45 2 1 117 1 113 3.72e-39 133
MsaG039686 AT5G63090.1 58.120 117 45 2 1 117 1 113 3.72e-39 133
MsaG039686 AT3G27650.1 56.481 108 46 1 8 115 33 139 3.75e-38 130
MsaG039686 AT2G30130.1 57.009 107 44 2 10 115 3 108 8.40e-37 127
MsaG039686 AT1G31320.1 58.696 92 37 1 10 101 9 99 4.63e-36 125
MsaG039686 AT2G30340.1 46.970 132 68 2 1 131 39 169 5.94e-36 127
MsaG039686 AT2G30340.2 46.970 132 68 2 1 131 40 170 7.45e-36 127
MsaG039686 AT1G07900.1 53.398 103 47 1 13 115 32 133 1.58e-34 122
MsaG039686 AT5G66870.1 39.884 173 88 4 9 180 2 159 8.15e-34 123
MsaG039686 AT2G28500.1 53.398 103 47 1 13 115 54 155 1.05e-33 121
MsaG039686 AT3G11090.1 46.457 127 66 2 6 131 3 128 1.77e-33 118
MsaG039686 AT1G65620.1 59.091 110 44 1 7 116 2 110 9.52e-33 117
MsaG039686 AT1G65620.4 59.091 110 44 1 7 116 2 110 9.52e-33 117
MsaG039686 AT1G65620.3 59.091 110 44 1 7 116 2 110 9.52e-33 117
MsaG039686 AT1G65620.5 59.091 110 44 1 7 116 2 110 9.52e-33 117
MsaG039686 AT1G65620.2 59.091 110 44 1 7 116 2 110 9.52e-33 117
MsaG039686 AT1G16530.1 51.852 108 50 2 11 117 11 117 1.02e-32 116
MsaG039686 AT2G23660.2 50.485 103 50 1 13 115 4 105 2.03e-31 117
MsaG039686 AT2G23660.3 50.485 103 50 1 13 115 4 105 2.03e-31 117
MsaG039686 AT2G23660.1 50.485 103 50 1 13 115 4 105 2.03e-31 117
MsaG039686 AT2G40470.2 56.190 105 45 1 11 115 17 120 3.56e-30 110
MsaG039686 AT2G40470.1 56.190 105 45 1 11 115 42 145 5.45e-30 111
MsaG039686 AT3G26660.1 44.118 102 56 1 15 116 6 106 5.44e-29 105
MsaG039686 AT3G26620.1 44.118 102 56 1 15 116 6 106 8.74e-29 105
MsaG039686 AT3G50510.1 46.667 105 55 1 12 116 10 113 1.44e-24 96.3
MsaG039686 AT3G50510.2 46.667 105 55 1 12 116 10 113 1.44e-24 96.3
MsaG039686 AT3G47870.1 38.679 106 64 1 10 115 32 136 1.71e-20 88.2
MsaG039686 AT3G13850.1 42.157 102 58 1 15 116 37 137 2.67e-20 86.7
MsaG039686 AT5G35900.1 38.532 109 66 1 15 123 6 113 4.21e-18 79.7
MsaG039686 AT1G72980.1 37.037 108 67 1 8 115 7 113 2.09e-17 77.8
MsaG039686 AT1G06280.1 41.772 79 37 2 15 89 25 98 3.15e-14 69.3
MsaG039686 AT4G22700.1 42.529 87 45 3 15 100 6 88 1.97e-12 63.9

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATGTAAGTTGGACAAAA+TGG 0.286192 7:-91909554 None:intergenic
AGTGAACTTTGAGGAGATAT+AGG 0.327596 7:-91909578 None:intergenic
TGAATAGCTGCAAATCTTGC+AGG 0.329259 7:-91908720 None:intergenic
ATCAATATGGCTTCTTCAAA+TGG 0.350219 7:+91908594 None:intergenic
GCAGCTATTCATAAAGTGTT+TGG 0.364830 7:+91908732 MsaT039686.1:CDS
TGTTGTCACAATTGCTTATG+AGG 0.389067 7:+91908812 MsaT039686.1:CDS
CGTATTAGAGACCCTGTATA+TGG 0.390387 7:+91908843 MsaT039686.1:CDS
GTGAACTTTGAGGAGATATA+GGG 0.398352 7:-91909577 None:intergenic
TATGCAGGTAGCATGCTTGC+AGG 0.403290 7:+91909427 MsaT039686.1:intron
GGAAACAATAATAATGTTGC+TGG 0.425208 7:+91909512 MsaT039686.1:CDS
GATATAGGGTTCATGTAAGT+TGG 0.426071 7:-91909563 None:intergenic
GATGACTCAAGTGAACTTTG+AGG 0.443078 7:-91909587 None:intergenic
TGAGAGACACAGCCATATAC+AGG 0.444019 7:-91908855 None:intergenic
TCTTCAAATGGAACTAGCTC+TGG 0.444575 7:+91908606 MsaT039686.1:CDS
TCTTATAATTATTACAACCT+TGG 0.449925 7:-91909688 None:intergenic
CAATAATAATGATCATGACT+TGG 0.461238 7:+91909727 MsaT039686.1:CDS
GAGAATAATAATCAATGGAC+AGG 0.471919 7:+91909491 MsaT039686.1:CDS
ATTGTGTACCTGTTGTTGTA+AGG 0.501180 7:-91908886 None:intergenic
CATAGCAGCAGCATGAATGA+TGG 0.514545 7:+91909617 MsaT039686.1:CDS
ACATAGAGAATAATAATCAA+TGG 0.518649 7:+91909486 MsaT039686.1:CDS
CCTTGTTCTGAGCAAAAGTA+AGG 0.518854 7:-91908696 None:intergenic
AATGGAACTAGCTCTGGAAC+TGG 0.534733 7:+91908612 MsaT039686.1:CDS
AGAGAAGATTTCTCATTCCA+AGG 0.536983 7:+91909671 MsaT039686.1:CDS
AATAATAATGATCATGACTT+GGG 0.555968 7:+91909728 MsaT039686.1:CDS
GCTTCACAACGTTCATGAGC+TGG 0.566405 7:-91908789 None:intergenic
TCTGGAACTGGTTCTCCTTG+TGG 0.567515 7:+91908624 MsaT039686.1:CDS
AAATAGCACTTAGGATGATG+AGG 0.594424 7:+91909762 MsaT039686.1:CDS
GAATAGCTGCAAATCTTGCA+GGG 0.610026 7:-91908719 None:intergenic
AGGAACTTGCATGCTCCACA+AGG 0.611585 7:-91908639 None:intergenic
AATTGCAAGAAATAGCACTT+AGG 0.616097 7:+91909753 MsaT039686.1:CDS
GAGAGACACAGCCATATACA+GGG 0.671769 7:-91908854 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 91908600 91909790 91908600 ID=MsaG039686
Chr7 mRNA 91908600 91909790 91908600 ID=MsaT039686.1;Parent=MsaG039686
Chr7 exon 91908600 91908899 91908600 ID=MsaT039686.1.exon1;Parent=MsaT039686.1
Chr7 CDS 91908600 91908899 91908600 ID=cds.MsaT039686.1;Parent=MsaT039686.1
Chr7 exon 91909434 91909790 91909434 ID=MsaT039686.1.exon2;Parent=MsaT039686.1
Chr7 CDS 91909434 91909790 91909434 ID=cds.MsaT039686.1;Parent=MsaT039686.1
Gene Sequence

>MsaT039686.1

ATGGCTTCTTCAAATGGAACTAGCTCTGGAACTGGTTCTCCTTGTGGAGCATGCAAGTTCCTTAGAAGAAAATGTGCACCTGATTGCATTTTTGCACCTTACTTTTGCTCAGAACAAGGCCCTGCAAGATTTGCAGCTATTCATAAAGTGTTTGGTGCTAGCAATGTTTCTAAGTTGCTTTTGCATATACCAGCTCATGAACGTTGTGAAGCTGTTGTCACAATTGCTTATGAGGCTCAAGCTCGTATTAGAGACCCTGTATATGGCTGTGTCTCTCACATTTTTGCCTTACAACAACAGGTAGCATGCTTGCAGGCACAATTGATGCAAGTGAAATCTCAACTGTCACATAACATAGAGAATAATAATCAATGGACAGGAAACAATAATAATGTTGCTGGCACACAAGCAATGAATTATCCATTTTGTCCAACTTACATGAACCCTATATCTCCTCAAAGTTCACTTGAGTCATCAATTGATCATAGCAGCAGCATGAATGATGGAATGATGAGCATGCAAGATGTACAAAGTAGCAGAGAAGATTTCTCATTCCAAGGTTGTAATAATTATAAGAAAAGAATGTCACATAACAATAATAATGATCATGACTTGGGTGAATTGCAAGAAATAGCACTTAGGATGATGAGGAACTAA
Protein sequence

>MsaT039686.1

MASSNGTSSGTGSPCGACKFLRRKCAPDCIFAPYFCSEQGPARFAAIHKVFGASNVSKLLLHIPAHERCEAVVTIAYEAQARIRDPVYGCVSHIFALQQQVACLQAQLMQVKSQLSHNIENNNQWTGNNNNVAGTQAMNYPFCPTYMNPISPQSSLESSIDHSSSMNDGMMSMQDVQSSREDFSFQGCNNYKKRMSHNNNNDHDLGELQEIALRMMRN*