Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039789 | AES81629.1 | 94.186 | 86 | 5 | 0 | 38 | 123 | 11 | 96 | 1.31e-50 | 166 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039789 | sp|F4JZT3|ARR24_ARATH | 39.167 | 120 | 69 | 2 | 16 | 135 | 18 | 133 | 4.09e-18 | 78.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039789 | tr|G7KYG8|G7KYG8_MEDTR | 94.186 | 86 | 5 | 0 | 38 | 123 | 11 | 96 | 6.27e-51 | 166 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG039789 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039789 | MtrunA17_Chr7g0262351 | 47.500 | 120 | 58 | 3 | 16 | 135 | 22 | 136 | 1.94e-25 | 94.0 |
| MsaG039789 | MtrunA17_Chr8g0364781 | 45.545 | 101 | 52 | 2 | 16 | 114 | 12 | 111 | 7.49e-18 | 74.7 |
| MsaG039789 | MtrunA17_Chr6g0472261 | 44.118 | 68 | 37 | 1 | 16 | 83 | 10 | 76 | 4.05e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039789 | AT5G26594.1 | 39.167 | 120 | 69 | 2 | 16 | 135 | 18 | 133 | 4.15e-19 | 78.2 |
| MsaG039789 | AT3G04280.1 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.63e-15 | 67.4 |
| MsaG039789 | AT3G04280.2 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.63e-15 | 67.4 |
| MsaG039789 | AT3G04280.3 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.63e-15 | 67.4 |
Find 32 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCGGGACTCTGTGATGATA+TGG | 0.319136 | 7:+93107766 | None:intergenic |
| GCCCGTCATGGATGGAATTA+CGG | 0.406314 | 7:-93107572 | MsaT039789.1:intron |
| CTGCATATTCATCATTAGTA+AGG | 0.410681 | 7:+93107268 | None:intergenic |
| AGAGATATCTTGACATTACT+TGG | 0.437895 | 7:-93107691 | MsaT039789.1:CDS |
| GAATACCCTGGCCAGATTGT+TGG | 0.444431 | 7:-93107303 | MsaT039789.1:CDS |
| AACCGTGCCAATGAGGTCTA+AGG | 0.458884 | 7:+93107633 | None:intergenic |
| TATGCAGAATTTCGCAATGC+AGG | 0.461823 | 7:-93107252 | MsaT039789.1:CDS |
| GACGTGAGACACCAACAATC+TGG | 0.490889 | 7:+93107292 | None:intergenic |
| GATGATCTACATCATGAAGA+TGG | 0.493143 | 7:-93107225 | MsaT039789.1:CDS |
| CCCGCGTGGATTAAAAGTAC+TGG | 0.511605 | 7:-93107749 | MsaT039789.1:CDS |
| GAGACACCAACAATCTGGCC+AGG | 0.513873 | 7:+93107297 | None:intergenic |
| AAAGAAGCCTTAGACCTCAT+TGG | 0.516268 | 7:-93107640 | MsaT039789.1:CDS |
| AGGATCATGCCCGTCATGGA+TGG | 0.527719 | 7:-93107580 | MsaT039789.1:CDS |
| TATCTTGACATTACTTGGTG+TGG | 0.530817 | 7:-93107686 | MsaT039789.1:CDS |
| TTTCAACATATGATAGTGCA+AGG | 0.532595 | 7:+93107197 | None:intergenic |
| AAAAGGTCATGCTAGTTGAC+AGG | 0.534345 | 7:+93107153 | None:intergenic |
| GGCTAGGATCATGCCCGTCA+TGG | 0.548604 | 7:-93107584 | MsaT039789.1:CDS |
| ACCGTAATTCCATCCATGAC+GGG | 0.549235 | 7:+93107571 | None:intergenic |
| TGGATTAAAAGTACTGGTTG+TGG | 0.556379 | 7:-93107743 | MsaT039789.1:CDS |
| ATGCAGAATTTCGCAATGCA+GGG | 0.581025 | 7:-93107251 | MsaT039789.1:CDS |
| TGATCCTAGCCATGAGAATG+AGG | 0.586084 | 7:+93107596 | None:intergenic |
| TAAATTCGACCTCATTCTCA+TGG | 0.588335 | 7:-93107605 | MsaT039789.1:CDS |
| TCGACCTCATTCTCATGGCT+AGG | 0.592585 | 7:-93107600 | MsaT039789.1:CDS |
| AACCGTAATTCCATCCATGA+CGG | 0.593130 | 7:+93107570 | None:intergenic |
| TACGTTCAACCGTGCCAATG+AGG | 0.601575 | 7:+93107626 | None:intergenic |
| AGCCTTAGACCTCATTGGCA+CGG | 0.608494 | 7:-93107635 | MsaT039789.1:CDS |
| AGATGATGATCTTGTTCGAA+TGG | 0.623757 | 7:-93107719 | MsaT039789.1:CDS |
| TTCAACATATGATAGTGCAA+GGG | 0.630612 | 7:+93107198 | None:intergenic |
| AGACACCAACAATCTGGCCA+GGG | 0.657108 | 7:+93107298 | None:intergenic |
| GGGACTCTGTGATGATATGG+AGG | 0.663830 | 7:+93107769 | None:intergenic |
| CTGCGCGATTTAGAATACCC+TGG | 0.695519 | 7:-93107315 | MsaT039789.1:CDS |
| TATCATCACAGAGTCCCGCG+TGG | 0.741838 | 7:-93107763 | MsaT039789.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 93107164 | 93107806 | 93107164 | ID=MsaG039789 |
| Chr7 | mRNA | 93107164 | 93107806 | 93107164 | ID=MsaT039789.1;Parent=MsaG039789 |
| Chr7 | exon | 93107164 | 93107349 | 93107164 | ID=MsaT039789.1.exon2;Parent=MsaT039789.1 |
| Chr7 | CDS | 93107164 | 93107349 | 93107164 | ID=cds.MsaT039789.1;Parent=MsaT039789.1 |
| Chr7 | exon | 93107573 | 93107806 | 93107573 | ID=MsaT039789.1.exon1;Parent=MsaT039789.1 |
| Chr7 | CDS | 93107573 | 93107806 | 93107573 | ID=cds.MsaT039789.1;Parent=MsaT039789.1 |
| Gene Sequence |
| Protein sequence |
