Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039821 | XP_003625472.1 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 2.30e-89 | 265 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039821 | sp|F4JZT3|ARR24_ARATH | 63.025 | 119 | 44 | 0 | 9 | 127 | 16 | 134 | 1.28e-47 | 152 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039821 | tr|G7KZK5|G7KZK5_MEDTR | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.10e-89 | 265 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG039821 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039821 | MtrunA17_Chr8g0364781 | 67.442 | 129 | 39 | 2 | 1 | 126 | 1 | 129 | 9.38e-55 | 168 |
| MsaG039821 | MtrunA17_Chr7g0262671 | 94.805 | 77 | 4 | 0 | 1 | 77 | 1 | 77 | 1.93e-49 | 153 |
| MsaG039821 | MtrunA17_Chr7g0262351 | 59.200 | 125 | 50 | 1 | 5 | 129 | 16 | 139 | 7.51e-46 | 145 |
| MsaG039821 | MtrunA17_Chr7g0262681 | 93.548 | 62 | 4 | 0 | 69 | 130 | 5 | 66 | 7.57e-37 | 120 |
| MsaG039821 | MtrunA17_Chr6g0472261 | 54.054 | 74 | 34 | 0 | 4 | 77 | 3 | 76 | 1.11e-21 | 82.0 |
| MsaG039821 | MtrunA17_Chr6g0451941 | 34.211 | 114 | 75 | 0 | 11 | 124 | 33 | 146 | 2.47e-18 | 75.9 |
| MsaG039821 | MtrunA17_Chr8g0392301 | 27.820 | 133 | 79 | 1 | 14 | 129 | 864 | 996 | 1.38e-12 | 63.5 |
| MsaG039821 | MtrunA17_Chr5g0447641 | 41.791 | 67 | 39 | 0 | 14 | 80 | 1077 | 1143 | 1.22e-11 | 60.8 |
| MsaG039821 | MtrunA17_Chr8g0371601 | 30.597 | 134 | 72 | 3 | 11 | 123 | 1029 | 1162 | 1.74e-11 | 60.1 |
| MsaG039821 | MtrunA17_Chr5g0405701 | 30.000 | 150 | 74 | 4 | 11 | 129 | 1050 | 1199 | 7.46e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039821 | AT5G26594.1 | 63.025 | 119 | 44 | 0 | 9 | 127 | 16 | 134 | 1.30e-48 | 152 |
| MsaG039821 | AT3G04280.1 | 46.789 | 109 | 56 | 2 | 11 | 117 | 22 | 130 | 6.63e-26 | 95.1 |
| MsaG039821 | AT3G04280.2 | 46.789 | 109 | 56 | 2 | 11 | 117 | 22 | 130 | 6.63e-26 | 95.1 |
| MsaG039821 | AT3G04280.3 | 46.789 | 109 | 56 | 2 | 11 | 117 | 22 | 130 | 6.63e-26 | 95.1 |
| MsaG039821 | AT2G01830.5 | 30.657 | 137 | 82 | 2 | 2 | 125 | 922 | 1058 | 4.61e-13 | 65.1 |
| MsaG039821 | AT2G01830.2 | 30.657 | 137 | 82 | 2 | 2 | 125 | 933 | 1069 | 5.04e-13 | 65.1 |
| MsaG039821 | AT2G01830.1 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.28e-13 | 65.1 |
| MsaG039821 | AT2G01830.4 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.28e-13 | 65.1 |
| MsaG039821 | AT2G01830.6 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.28e-13 | 65.1 |
| MsaG039821 | AT2G01830.3 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.28e-13 | 65.1 |
| MsaG039821 | AT5G35750.1 | 28.889 | 135 | 74 | 2 | 14 | 126 | 1038 | 1172 | 1.63e-12 | 63.5 |
Find 31 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAAGATGTTGAATAAATT+TGG | 0.144966 | 7:+93506733 | MsaT039821.1:CDS |
| GCAATACTTAGTTCCATTCT+TGG | 0.228362 | 7:+93508084 | MsaT039821.1:CDS |
| TATCCATGTCCATGAGAATT+AGG | 0.311699 | 7:-93506825 | None:intergenic |
| TGGATTGATCTTGCCAAGAA+TGG | 0.418939 | 7:-93508097 | None:intergenic |
| CACAACCAGTGCTGTTAGCT+TGG | 0.421611 | 7:-93506680 | None:intergenic |
| AAAATTCATGGAAGCTGGAT+TGG | 0.421768 | 7:+93508032 | MsaT039821.1:CDS |
| TGGTGTGTCATCACGCTCTA+TGG | 0.423024 | 7:+93507996 | MsaT039821.1:CDS |
| AGATGAAATACTAAAATTCA+TGG | 0.428022 | 7:+93508020 | MsaT039821.1:CDS |
| ACTAAGTATTGCATTGTTCA+AGG | 0.440010 | 7:-93508073 | None:intergenic |
| AAGAAGCAGTGGACATTCAT+TGG | 0.445266 | 7:+93506785 | MsaT039821.1:CDS |
| ATACTAAAATTCATGGAAGC+TGG | 0.448559 | 7:+93508027 | MsaT039821.1:CDS |
| GGTATTTGTAGCACAATTGT+TGG | 0.475026 | 7:+93507976 | MsaT039821.1:CDS |
| AAAATTTGACCTAATTCTCA+TGG | 0.477878 | 7:+93506816 | MsaT039821.1:CDS |
| AAAAGTCAAGGTGTGGAGAA+TGG | 0.478580 | 7:+93506760 | MsaT039821.1:CDS |
| ACAAAGGAACTTCGTGCAAT+GGG | 0.482431 | 7:+93507955 | MsaT039821.1:CDS |
| CAAGCTAACAGCACTGGTTG+TGG | 0.482562 | 7:+93506681 | MsaT039821.1:CDS |
| ACCTCAATGCCATTCATGAT+AGG | 0.492567 | 7:-93506850 | None:intergenic |
| CCTTGCATCCATCACACAGT+TGG | 0.511788 | 7:-93508117 | None:intergenic |
| AACAAAGGAACTTCGTGCAA+TGG | 0.518430 | 7:+93507954 | MsaT039821.1:CDS |
| ATGGATATGCCTATCATGAA+TGG | 0.542735 | 7:+93506841 | MsaT039821.1:CDS |
| CTAAGTATTGCATTGTTCAA+GGG | 0.546876 | 7:-93508072 | None:intergenic |
| CAGTGGACATTCATTGGCGT+GGG | 0.559565 | 7:+93506791 | MsaT039821.1:CDS |
| GCCTATCATGAATGGCATTG+AGG | 0.559799 | 7:+93506849 | MsaT039821.1:CDS |
| TGTATCCAAGCTAACAGCAC+TGG | 0.575373 | 7:+93506675 | MsaT039821.1:CDS |
| TGTGTTTATATAGGCAACAA+AGG | 0.581181 | 7:+93507939 | MsaT039821.1:intron |
| AGATCAATCCAACTGTGTGA+TGG | 0.583988 | 7:+93508109 | MsaT039821.1:CDS |
| TGACCTAATTCTCATGGACA+TGG | 0.592027 | 7:+93506822 | MsaT039821.1:CDS |
| GCAGTGGACATTCATTGGCG+TGG | 0.597898 | 7:+93506790 | MsaT039821.1:CDS |
| AAATTTGGAGTCAAAAGTCA+AGG | 0.637368 | 7:+93506748 | MsaT039821.1:CDS |
| TGGAGTCAAAAGTCAAGGTG+TGG | 0.637883 | 7:+93506753 | MsaT039821.1:CDS |
| GGAGAATGGAAAAGAAGCAG+TGG | 0.653869 | 7:+93506774 | MsaT039821.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 93506655 | 93508128 | 93506655 | ID=MsaG039821 |
| Chr7 | mRNA | 93506655 | 93508128 | 93506655 | ID=MsaT039821.1;Parent=MsaG039821 |
| Chr7 | exon | 93506655 | 93506870 | 93506655 | ID=MsaT039821.1.exon1;Parent=MsaT039821.1 |
| Chr7 | CDS | 93506655 | 93506870 | 93506655 | ID=cds.MsaT039821.1;Parent=MsaT039821.1 |
| Chr7 | exon | 93507952 | 93508128 | 93507952 | ID=MsaT039821.1.exon2;Parent=MsaT039821.1 |
| Chr7 | CDS | 93507952 | 93508128 | 93507952 | ID=cds.MsaT039821.1;Parent=MsaT039821.1 |
| Gene Sequence |
| Protein sequence |
