AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG039822

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039822 MtrunA17_Chr7g0262691 87.835 485 30 7 2 479 3 465 0.0 798
MsaG039822 MtrunA17_Chr1g0182791 54.802 531 169 24 1 469 1 522 1.57e-146 432
MsaG039822 MtrunA17_Chr3g0095391 32.720 489 226 18 22 452 21 464 1.16e-53 188
MsaG039822 MtrunA17_Chr1g0193321 57.432 148 52 4 210 352 403 544 9.18e-38 148
MsaG039822 MtrunA17_Chr7g0271251 43.689 206 95 5 194 390 357 550 3.01e-32 131
MsaG039822 MtrunA17_Chr1g0153241 70.513 78 21 1 272 347 85 162 2.80e-27 110
MsaG039822 MtrunA17_Chr5g0402031 76.119 67 14 1 281 345 114 180 1.34e-24 105
MsaG039822 MtrunA17_Chr7g0270871 50.400 125 46 5 264 375 186 307 2.25e-24 105
MsaG039822 MtrunA17_Chr7g0231461 57.500 80 34 0 256 335 281 360 4.06e-22 99.8
MsaG039822 MtrunA17_Chr4g0074161 49.462 93 38 2 275 358 209 301 1.79e-18 87.8
MsaG039822 MtrunA17_Chr1g0190731 50.000 78 35 1 275 348 196 273 3.01e-17 83.6
MsaG039822 MtrunA17_Chr1g0152131 35.172 145 85 4 238 376 229 370 1.64e-14 75.9
MsaG039822 MtrunA17_Chr5g0393721 39.000 100 51 2 269 367 220 310 2.44e-14 74.3
MsaG039822 MtrunA17_Chr7g0234761 31.319 182 109 6 171 341 211 387 3.29e-14 75.1
MsaG039822 MtrunA17_Chr3g0144641 37.719 114 55 3 275 374 194 305 5.63e-14 73.9
MsaG039822 MtrunA17_Chr8g0339091 38.136 118 62 2 235 341 244 361 3.47e-13 72.0
MsaG039822 MtrunA17_Chr4g0035101 49.275 69 34 1 274 341 145 213 7.99e-13 70.1
MsaG039822 MtrunA17_Chr3g0131771 48.611 72 36 1 275 345 150 221 8.35e-13 69.7
MsaG039822 MtrunA17_Chr1g0207311 33.333 102 54 2 275 371 194 286 8.41e-13 69.3
MsaG039822 MtrunA17_Chr5g0415291 31.013 158 105 3 198 353 40 195 9.94e-13 68.2
MsaG039822 MtrunA17_Chr8g0363921 50.000 64 31 1 274 336 153 216 1.14e-12 68.6
MsaG039822 MtrunA17_Chr1g0174161 35.537 121 66 4 274 392 162 272 1.22e-12 69.3
MsaG039822 MtrunA17_Chr8g0385911 34.146 123 77 2 275 393 148 270 1.32e-12 68.9
MsaG039822 MtrunA17_Chr3g0134741 30.882 136 78 3 245 375 197 321 1.60e-12 68.6
MsaG039822 MtrunA17_Chr8g0365941 37.209 129 67 4 274 389 307 434 1.63e-12 69.7
MsaG039822 MtrunA17_Chr5g0421111 36.508 126 67 4 275 388 368 492 1.66e-12 70.1
MsaG039822 MtrunA17_Chr7g0258161 44.928 69 37 1 274 341 175 243 4.46e-11 64.7
MsaG039822 MtrunA17_Chr6g0484271 43.243 74 41 1 264 336 147 220 6.16e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039822 AT2G20180.4 42.159 491 171 27 21 460 26 454 3.62e-69 230
MsaG039822 AT2G20180.6 42.159 491 171 27 21 460 26 454 3.62e-69 230
MsaG039822 AT2G20180.2 42.159 491 171 27 21 460 26 454 3.62e-69 230
MsaG039822 AT2G20180.3 42.159 491 171 27 21 460 26 454 3.62e-69 230
MsaG039822 AT2G20180.7 43.333 420 151 22 82 460 10 383 1.15e-61 208
MsaG039822 AT2G20180.1 43.333 420 151 22 82 460 10 383 1.15e-61 208
MsaG039822 AT2G20180.5 43.333 420 151 22 82 460 10 383 1.15e-61 208
MsaG039822 AT4G28800.1 34.899 447 216 17 25 447 16 411 3.65e-54 190
MsaG039822 AT2G20180.8 42.330 352 126 19 21 333 26 339 4.40e-51 178
MsaG039822 AT4G28800.2 34.086 443 213 17 28 447 31 417 6.83e-50 178
MsaG039822 AT4G28790.1 35.401 387 192 14 18 375 14 371 9.34e-45 163
MsaG039822 AT4G28811.1 35.106 470 238 18 27 467 107 538 1.24e-35 140
MsaG039822 AT4G28790.2 33.628 339 180 10 18 332 14 331 2.09e-34 133
MsaG039822 AT1G09530.3 61.017 118 38 3 242 351 303 420 8.65e-33 132
MsaG039822 AT1G09530.2 61.017 118 38 3 242 351 303 420 8.65e-33 132
MsaG039822 AT1G09530.5 61.017 118 38 3 242 351 303 420 8.65e-33 132
MsaG039822 AT1G09530.1 61.017 118 38 3 242 351 303 420 8.65e-33 132
MsaG039822 AT1G09530.4 61.017 118 38 3 242 351 303 420 8.65e-33 132
MsaG039822 AT1G09530.6 61.017 118 38 3 242 351 303 420 8.65e-33 132
MsaG039822 AT2G46970.2 43.293 164 77 5 241 394 157 314 7.53e-30 121
MsaG039822 AT2G46970.3 43.293 164 77 5 241 394 166 323 9.19e-30 121
MsaG039822 AT2G46970.1 43.293 164 77 5 241 394 188 345 1.13e-29 121
MsaG039822 AT3G59060.3 54.054 148 51 6 240 372 212 357 1.70e-28 118
MsaG039822 AT3G59060.2 54.054 148 51 6 240 372 212 357 1.70e-28 118
MsaG039822 AT3G59060.4 54.054 148 51 6 240 372 212 357 1.70e-28 118
MsaG039822 AT3G59060.1 54.054 148 51 6 240 372 212 357 1.83e-28 118
MsaG039822 AT4G36930.1 60.185 108 36 2 247 347 161 268 5.45e-27 112
MsaG039822 AT4G28815.2 39.572 187 68 6 271 455 67 210 1.27e-26 108
MsaG039822 AT4G28815.1 39.785 186 67 6 272 455 144 286 5.62e-26 108
MsaG039822 AT2G43010.4 56.911 123 41 3 234 349 217 334 1.08e-25 109
MsaG039822 AT2G43010.2 56.911 123 41 3 234 349 217 334 1.30e-25 109
MsaG039822 AT2G43010.1 56.911 123 41 3 234 349 217 334 1.36e-25 109
MsaG039822 AT2G43010.3 56.911 123 41 3 234 349 224 341 1.44e-25 109
MsaG039822 AT2G43010.5 56.911 123 41 3 234 349 259 376 1.69e-25 110
MsaG039822 AT5G67110.1 64.103 78 26 1 273 348 88 165 4.73e-25 103
MsaG039822 AT4G00050.2 59.756 82 30 1 254 335 90 168 1.23e-22 99.0
MsaG039822 AT4G00050.3 59.756 82 30 1 254 335 192 270 2.63e-22 99.4
MsaG039822 AT4G00050.1 59.756 82 30 1 254 335 192 270 2.83e-22 99.8
MsaG039822 AT3G62090.1 44.697 132 65 4 274 401 167 294 4.37e-21 95.1
MsaG039822 AT3G62090.2 44.697 132 65 4 274 401 184 311 7.08e-21 94.7
MsaG039822 AT2G24260.2 62.903 62 23 0 275 336 38 99 1.05e-19 89.0
MsaG039822 AT2G24260.1 62.903 62 23 0 275 336 141 202 5.01e-19 89.4
MsaG039822 AT4G30980.1 61.290 62 24 0 275 336 133 194 1.07e-18 87.4
MsaG039822 AT4G30980.2 61.290 62 24 0 275 336 181 242 2.63e-18 87.0
MsaG039822 AT5G58010.1 51.282 78 34 1 275 348 102 179 1.22e-17 84.3
MsaG039822 AT5G50915.3 28.019 207 128 5 202 389 57 261 2.81e-15 76.6
MsaG039822 AT5G50915.4 28.019 207 128 5 202 389 57 261 2.81e-15 76.6
MsaG039822 AT5G50915.1 28.019 207 128 5 202 389 57 261 5.24e-15 76.3
MsaG039822 AT5G50915.2 28.019 207 128 5 202 389 57 261 5.24e-15 76.3
MsaG039822 AT5G61270.2 40.171 117 62 2 233 348 40 149 1.36e-14 74.7
MsaG039822 AT5G61270.3 40.171 117 62 2 233 348 40 149 1.36e-14 74.7
MsaG039822 AT5G61270.1 40.171 117 62 2 233 348 128 237 1.72e-14 75.5
MsaG039822 AT3G23690.1 39.286 112 63 3 234 341 151 261 8.77e-14 73.6
MsaG039822 AT1G59640.2 32.370 173 102 5 187 353 56 219 1.04e-13 73.2
MsaG039822 AT1G59640.1 46.341 82 42 2 274 353 138 219 1.34e-13 71.6
MsaG039822 AT1G02340.2 38.333 120 51 6 286 398 134 237 1.61e-13 71.6
MsaG039822 AT1G02340.1 38.333 120 51 6 286 398 141 244 1.97e-13 71.6
MsaG039822 AT4G28790.6 28.814 295 165 10 18 288 14 287 2.87e-13 71.2
MsaG039822 AT4G28790.3 28.814 295 165 10 18 288 14 287 2.87e-13 71.2
MsaG039822 AT4G28790.4 28.814 295 165 10 18 288 14 287 2.87e-13 71.2
MsaG039822 AT3G07340.2 36.290 124 69 3 219 341 214 328 6.81e-13 71.2
MsaG039822 AT1G10120.2 42.857 91 47 2 256 341 186 276 7.34e-13 70.5
MsaG039822 AT1G10120.1 42.857 91 47 2 256 341 186 276 7.34e-13 70.5
MsaG039822 AT4G36540.2 33.333 126 60 3 231 341 95 211 9.68e-13 69.7
MsaG039822 AT4G36540.1 33.333 126 60 3 231 341 95 211 9.79e-13 69.7
MsaG039822 AT2G14760.1 35.922 103 54 3 268 368 232 324 1.09e-12 69.7
MsaG039822 AT3G07340.1 36.290 124 69 3 219 341 214 328 1.19e-12 70.5
MsaG039822 AT5G48560.2 50.000 68 33 1 275 341 304 371 1.51e-12 70.1
MsaG039822 AT1G68920.3 50.000 68 33 1 275 341 305 372 1.56e-12 70.5
MsaG039822 AT1G68920.1 50.000 68 33 1 275 341 306 373 1.57e-12 70.5
MsaG039822 AT1G68920.4 50.000 68 33 1 275 341 306 373 1.57e-12 70.5
MsaG039822 AT1G68920.2 50.000 68 33 1 275 341 306 373 1.57e-12 70.5
MsaG039822 AT5G48560.1 50.000 68 33 1 275 341 304 371 1.76e-12 70.1
MsaG039822 AT4G34530.1 45.588 68 36 1 275 341 175 242 2.51e-12 68.6
MsaG039822 AT5G62610.2 50.000 68 33 1 275 341 156 223 2.97e-12 67.8
MsaG039822 AT5G62610.1 50.000 68 33 1 275 341 156 223 2.97e-12 67.8
MsaG039822 AT1G26260.5 35.537 121 61 3 237 341 152 271 3.14e-12 68.9
MsaG039822 AT1G25330.1 45.070 71 38 1 267 336 99 169 3.38e-12 66.6
MsaG039822 AT1G26260.3 35.537 121 61 3 237 341 123 242 3.55e-12 68.2
MsaG039822 AT1G26260.4 35.537 121 61 3 237 341 174 293 3.73e-12 68.6
MsaG039822 AT1G26260.1 35.537 121 61 3 237 341 174 293 3.73e-12 68.6
MsaG039822 AT1G26260.6 35.537 121 61 3 237 341 174 293 3.73e-12 68.6
MsaG039822 AT1G26260.2 35.537 121 61 3 237 341 174 293 3.73e-12 68.6
MsaG039822 AT2G18300.2 45.588 68 36 1 275 341 188 255 7.48e-12 67.4
MsaG039822 AT2G14760.3 35.227 88 48 1 281 368 259 337 7.70e-12 67.4
MsaG039822 AT2G18300.1 45.588 68 36 1 275 341 188 255 8.15e-12 67.0
MsaG039822 AT2G18300.3 45.588 68 36 1 275 341 188 255 1.03e-11 67.0
MsaG039822 AT5G43175.1 33.696 92 52 1 276 367 136 218 1.79e-11 64.7
MsaG039822 AT3G57800.2 47.059 68 35 1 275 341 207 274 2.66e-11 65.9
MsaG039822 AT2G42300.4 45.588 68 36 1 275 341 188 255 3.33e-11 64.7
MsaG039822 AT4G33880.1 36.735 98 50 3 268 363 261 348 3.53e-11 65.5
MsaG039822 AT2G42300.1 45.588 68 36 1 275 341 188 255 5.11e-11 64.7
MsaG039822 AT1G73830.2 46.032 63 33 1 275 336 150 212 6.65e-11 63.5
MsaG039822 AT1G18400.2 46.032 63 33 1 275 336 148 210 6.66e-11 63.2
MsaG039822 AT1G73830.1 46.032 63 33 1 275 336 150 212 7.45e-11 63.2
MsaG039822 AT1G18400.1 46.032 63 33 1 275 336 148 210 9.28e-11 63.2

Find 160 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACCTGACTGGCGGGTTTC+TGG 0.085323 7:-93522115 None:intergenic
TGTCCTGTGTTATGGGTTTC+TGG 0.091136 7:+93522212 MsaT039822.1:CDS
TTTGCCTGCAGCCGCGAATT+TGG 0.127478 7:+93521525 MsaT039822.1:CDS
TGATAAGGATCACTGAAATT+TGG 0.152435 7:-93521685 None:intergenic
CTGATTTGCTGCTTGCATTC+TGG 0.157146 7:-93521605 None:intergenic
CAACCCTTTCTGAGAGATTA+TGG 0.251723 7:-93520423 None:intergenic
TTGCCTGCAGCCGCGAATTT+GGG 0.259006 7:+93521526 MsaT039822.1:CDS
ATAGCTCTTGTCCTGTGTTA+TGG 0.282189 7:+93522204 MsaT039822.1:intron
AAAACTTGGCATACTGTATT+AGG 0.288340 7:-93522602 None:intergenic
CTTCTACTCCCTTCCTCTTT+CGG 0.289745 7:-93518628 None:intergenic
CACCGAAGTGGCGGTGATTT+CGG 0.290963 7:+93518532 MsaT039822.1:CDS
AGTTACATGATTGGTTTCTT+AGG 0.305370 7:-93517888 None:intergenic
TAGCTCTTGTCCTGTGTTAT+GGG 0.311035 7:+93522205 MsaT039822.1:intron
AATGGGGATGGGTATGGAAA+TGG 0.320817 7:+93521453 MsaT039822.1:CDS
ATATTGGGAAATGGCATTAC+TGG 0.322002 7:-93521487 None:intergenic
GGGAGTAGAAGCGGAGGAAT+GGG 0.328574 7:+93518640 MsaT039822.1:CDS
GATAAGGATCACTGAAATTT+GGG 0.328825 7:-93521684 None:intergenic
GGCATATACTGCTGGATTCC+AGG 0.330279 7:-93521415 None:intergenic
ACTTATACCTCGATCTAATA+AGG 0.337045 7:+93520578 MsaT039822.1:CDS
TAACCGTCGGCGGCGGGTTA+AGG 0.340851 7:-93518241 None:intergenic
TCAAGAGGCCTGCTGACATT+TGG 0.353245 7:-93522088 None:intergenic
AAGAAAATGAAATGGCTTCA+TGG 0.355100 7:+93518171 MsaT039822.1:CDS
AGCAGAACCGGATAACATAT+TGG 0.356663 7:-93521503 None:intergenic
AGGGAGTAGAAGCGGAGGAA+TGG 0.360376 7:+93518639 MsaT039822.1:CDS
TTCTTCAGATGACGGTGGTT+TGG 0.379401 7:-93518044 None:intergenic
TTCAAAATTGACGACGGTCC+TGG 0.387225 7:-93518401 None:intergenic
ATGGGGATGGGTATGGAAAT+GGG 0.393065 7:+93521454 MsaT039822.1:CDS
ATGAGATCATGGAGCTGTTA+TGG 0.394587 7:+93517994 MsaT039822.1:CDS
CCATTTCCCAATATGTTATC+CGG 0.395626 7:+93521496 MsaT039822.1:CDS
CAGAATGCAAGCAGCAAATC+AGG 0.395981 7:+93521606 MsaT039822.1:CDS
TACGCGAAGTTCGGTGTCTC+AGG 0.398297 7:-93518350 None:intergenic
GGCTGCAGGCAAAGCAGAAC+CGG 0.401251 7:-93521515 None:intergenic
CGTGCCCTATCCTCCGTGGC+GGG 0.417127 7:-93518322 None:intergenic
AAGCGGAAAAGTTACATGAT+TGG 0.418255 7:-93517897 None:intergenic
TTCCGTTACACTACAAAACT+TGG 0.421479 7:-93522616 None:intergenic
GCAGAACCGGATAACATATT+GGG 0.422468 7:-93521502 None:intergenic
TTCATATTTCGAATCTCAAA+TGG 0.426691 7:+93517842 MsaT039822.1:CDS
GATCCTTATCAACAATATCT+TGG 0.428452 7:+93521697 MsaT039822.1:CDS
GGTCCCAAATTCGCGGCTGC+AGG 0.429443 7:-93521529 None:intergenic
AATGGGAATGGGGATGGGTA+TGG 0.431065 7:+93521447 MsaT039822.1:CDS
TATACTACTAGCTCTCAATA+TGG 0.434563 7:+93522465 MsaT039822.1:CDS
CAACTGTTGGCATATACTGC+TGG 0.435351 7:-93521423 None:intergenic
TTGTTATTAACCGTCGGCGG+CGG 0.436019 7:-93518248 None:intergenic
CTTCATGCAAGAAAATGAAA+TGG 0.444338 7:+93518163 MsaT039822.1:CDS
CCAACAGTTGGAATGGGAAT+GGG 0.446678 7:+93521436 MsaT039822.1:CDS
TGCCCTATCCTCCGTGGCGG+GGG 0.448845 7:-93518320 None:intergenic
ACATGGTAAACAACCAGATC+TGG 0.451429 7:-93522248 None:intergenic
CGGCGGCGGGTTAAGGAAAT+CGG 0.453500 7:-93518234 None:intergenic
GGTCCAAGATATTGTTGATA+AGG 0.461439 7:-93521700 None:intergenic
TGCTCCGCTACCTTCCCAAC+CGG 0.462532 7:-93518602 None:intergenic
CCGGATAACATATTGGGAAA+TGG 0.466866 7:-93521496 None:intergenic
CCCATTCCCATTCCAACTGT+TGG 0.469499 7:-93521436 None:intergenic
CGGGTTTCTGGTTTCTCAAG+AGG 0.469774 7:-93522103 None:intergenic
ATGGTACCTATGATGTTTCC+TGG 0.471988 7:+93521397 MsaT039822.1:CDS
TAGCAGTGAAGAAGCTGAAC+CGG 0.479213 7:+93518583 MsaT039822.1:CDS
GGTTGGGAAGGTAGCGGAGC+AGG 0.479306 7:+93518604 MsaT039822.1:CDS
GTTAAGGAAATCGGTGGAAA+AGG 0.482894 7:-93518225 None:intergenic
TTCGCAAAGTACGCGAAGTT+CGG 0.484693 7:-93518359 None:intergenic
TAGCGGAGCAGGACCGAAAG+AGG 0.484786 7:+93518615 MsaT039822.1:CDS
GTTGGAATGGGAATGGGGAT+GGG 0.486170 7:+93521442 MsaT039822.1:CDS
GACGATTCAACTCGCGGCGG+AGG 0.486968 7:+93518089 MsaT039822.1:CDS
AGTTGGAATGGGAATGGGGA+TGG 0.489416 7:+93521441 MsaT039822.1:CDS
AGCACAGTGACTTCCAGATC+TGG 0.489837 7:+93522235 MsaT039822.1:CDS
AAACCAGAAACCCGCCAGTC+AGG 0.490474 7:+93522112 MsaT039822.1:CDS
GCCAACAGTTGGAATGGGAA+TGG 0.490991 7:+93521435 MsaT039822.1:CDS
AACGCCGCCGGCATCACAGC+TGG 0.493297 7:-93518467 None:intergenic
GTGAAGAAGCTGAACCGGTT+GGG 0.494334 7:+93518588 MsaT039822.1:CDS
GTCGCTGCAGTTACAAGTGC+AGG 0.496663 7:+93520754 MsaT039822.1:CDS
AATAATGTGATCACCTCAGC+TGG 0.496940 7:+93521634 MsaT039822.1:CDS
GCATCAATTGAAACTGCATC+TGG 0.498748 7:-93521726 None:intergenic
TCGCGGCGGAGGAGCTTCAT+CGG 0.501663 7:+93518100 MsaT039822.1:CDS
CTATGCAGAGGCGTCGTGAT+CGG 0.503935 7:+93520526 MsaT039822.1:intron
TGGATTCCAGGAAACATCAT+AGG 0.504622 7:-93521403 None:intergenic
TGTTATTAACCGTCGGCGGC+GGG 0.505645 7:-93518247 None:intergenic
CAGTGCAGATGATGAGATCA+TGG 0.509755 7:+93517983 MsaT039822.1:intron
GAGGCCTGCTGACATTTGGT+TGG 0.510685 7:-93522084 None:intergenic
GTCTGACAAAGCATCTATGC+TGG 0.514190 7:+93520709 MsaT039822.1:intron
CGGAGGAGCTTCATCGGCGA+GGG 0.525295 7:+93518106 MsaT039822.1:CDS
GAGGAAGGGAGTAGAAGCGG+AGG 0.530303 7:+93518634 MsaT039822.1:CDS
TTTCCTTAACCCGCCGCCGA+CGG 0.530560 7:+93518238 MsaT039822.1:CDS
GTATATGCCAACAGTTGGAA+TGG 0.532323 7:+93521429 MsaT039822.1:CDS
GGAGCAGGACCGAAAGAGGA+AGG 0.532955 7:+93518619 MsaT039822.1:CDS
CACGGTGTAAGAACCGAGCC+AGG 0.537878 7:+93518383 MsaT039822.1:CDS
GATAAGGCAATTTACCTGAC+TGG 0.538094 7:-93522126 None:intergenic
AAAGAGGAAGGGAGTAGAAG+CGG 0.541766 7:+93518631 MsaT039822.1:CDS
GTGGAAAAGGTTTGTTCGAA+AGG 0.542709 7:-93518212 None:intergenic
TTGGCCTCTGTGTAACGACT+TGG 0.544571 7:-93518025 None:intergenic
TATATGCCAACAGTTGGAAT+GGG 0.544698 7:+93521430 MsaT039822.1:CDS
GGAGACGGTAAGAAGTTTAG+CGG 0.545838 7:+93518493 MsaT039822.1:CDS
GATGCAGTTTCAATTGATGC+AGG 0.546912 7:+93521729 MsaT039822.1:CDS
AAGAAGTTTAGCGGATCAAA+CGG 0.547113 7:+93518502 MsaT039822.1:CDS
AGGCAATTTACCTGACTGGC+GGG 0.549682 7:-93522122 None:intergenic
TCTCAAATGGATCAACAAGC+AGG 0.550538 7:+93517855 MsaT039822.1:CDS
CAGCAGTATATGCCAACAGT+TGG 0.551794 7:+93521424 MsaT039822.1:CDS
GTGCCCTATCCTCCGTGGCG+GGG 0.557747 7:-93518321 None:intergenic
ACTTATGCAGATGATGTCCA+TGG 0.558617 7:+93521366 MsaT039822.1:intron
GGTCCATAATCTCTCAGAAA+GGG 0.559468 7:+93520420 MsaT039822.1:CDS
ATGATTGGTTTCTTAGGACG+AGG 0.561500 7:-93517882 None:intergenic
AATTGACGACGGTCCTGGCT+CGG 0.563062 7:-93518396 None:intergenic
GAGCAGGACCGAAAGAGGAA+GGG 0.563277 7:+93518620 MsaT039822.1:CDS
TTCGCGTACTTTGCGAAGCA+CGG 0.563359 7:+93518365 MsaT039822.1:CDS
TGAACCAACCAAATGTCAGC+AGG 0.563946 7:+93522080 MsaT039822.1:CDS
GCTTGCATTCTGGATGAACC+AGG 0.565601 7:-93521595 None:intergenic
ACCGTCTCCGAAAACGCCGC+CGG 0.567574 7:-93518479 None:intergenic
GGAACATGCGGCATGTGGAA+AGG 0.567580 7:-93521568 None:intergenic
AAAGAGATCTCGTGCTGCTG+AGG 0.571066 7:+93520399 MsaT039822.1:CDS
AGTGAAGAAGCTGAACCGGT+TGG 0.572237 7:+93518587 MsaT039822.1:CDS
AAAGGAGGCATCACGAACCT+TGG 0.572272 7:-93521550 None:intergenic
CAACAGTTGGAATGGGAATG+GGG 0.575101 7:+93521437 MsaT039822.1:CDS
ATGCCGCATGTTCCTACACC+TGG 0.577972 7:+93521577 MsaT039822.1:CDS
CAATTGAAACTGCATCTGGT+GGG 0.578724 7:-93521722 None:intergenic
TACAATACAAACTCCGACGT+CGG 0.578904 7:+93518274 MsaT039822.1:CDS
GAACCTTGGTCCCAAATTCG+CGG 0.579140 7:-93521536 None:intergenic
AGGTACCATGCCACATCCCA+TGG 0.582211 7:-93521383 None:intergenic
GTGTAGGAACATGCGGCATG+TGG 0.583623 7:-93521573 None:intergenic
GCGGAGGAGCTTCATCGGCG+AGG 0.587970 7:+93518105 MsaT039822.1:CDS
TGCCGAAATCACCGCCACTT+CGG 0.593010 7:-93518534 None:intergenic
AATTGAAACTGCATCTGGTG+GGG 0.594089 7:-93521721 None:intergenic
TCAAGGGCTGGCTATGCAGA+TGG 0.595639 7:+93522417 MsaT039822.1:intron
GCAGTGGTTTCTTCAGATGA+CGG 0.597213 7:-93518052 None:intergenic
CAGTTTACCTTATTAGATCG+AGG 0.600812 7:-93520585 None:intergenic
CAGATGATGTCCATGGGATG+TGG 0.601497 7:+93521373 MsaT039822.1:intron
AGAAGCTGAACCGGTTGGGA+AGG 0.602490 7:+93518592 MsaT039822.1:CDS
AGGTCCATAATCTCTCAGAA+AGG 0.604467 7:+93520419 MsaT039822.1:CDS
CAGCCGCGAATTTGGGACCA+AGG 0.608681 7:+93521533 MsaT039822.1:CDS
GTACTGTTGTTATTAACCGT+CGG 0.610377 7:-93518254 None:intergenic
TGATACACCAGCTGTGATGC+CGG 0.610769 7:+93518460 MsaT039822.1:CDS
TCCGCCGCGAGTTGAATCGT+CGG 0.611337 7:-93518087 None:intergenic
ACATGCGGCATGTGGAAAGG+AGG 0.621566 7:-93521565 None:intergenic
ATTCTGGATGAACCAGGTGT+AGG 0.621664 7:-93521589 None:intergenic
AATACAAACTCCGACGTCGG+TGG 0.622484 7:+93518277 MsaT039822.1:CDS
TGAATCATGTGTTCCAGCTG+AGG 0.623133 7:-93521647 None:intergenic
GAACCAGGTGTAGGAACATG+CGG 0.626770 7:-93521580 None:intergenic
AACTCCGACGATTCAACTCG+CGG 0.628283 7:+93518083 MsaT039822.1:CDS
TCAATTGAAACTGCATCTGG+TGG 0.633505 7:-93521723 None:intergenic
AAGGCAATTTACCTGACTGG+CGG 0.635377 7:-93522123 None:intergenic
TGAACCGGTTGGGAAGGTAG+CGG 0.635941 7:+93518598 MsaT039822.1:CDS
TCATCTGAAGAAACCACTGC+CGG 0.639590 7:+93518055 MsaT039822.1:CDS
AGTTTAGCGGATCAAACGGA+AGG 0.644672 7:+93518506 MsaT039822.1:CDS
GATGTCCATGGGATGTGGCA+TGG 0.651695 7:+93521378 MsaT039822.1:CDS
TGCGAGGGAATCAACAGTGG+TGG 0.655914 7:+93518430 MsaT039822.1:CDS
GGAAGGAGACACCGAAGTGG+CGG 0.656231 7:+93518523 MsaT039822.1:CDS
GTGGTTTCTTCAGATGACGG+TGG 0.658476 7:-93518049 None:intergenic
ATAGAATTACTTCATTGACA+TGG 0.663562 7:-93522265 None:intergenic
CGGCGGGTTAAGGAAATCGG+TGG 0.664688 7:-93518231 None:intergenic
TTGGCATACTGTATTAGGAG+TGG 0.665219 7:-93522597 None:intergenic
TCACTGCTACTTGAACCGCC+CGG 0.678494 7:-93518569 None:intergenic
ACGTGCCCTATCCTCCGTGG+CGG 0.679829 7:-93518323 None:intergenic
CGAGAAGGAACCACCGACGT+CGG 0.693171 7:-93518287 None:intergenic
TGCACGTGCCCTATCCTCCG+TGG 0.693280 7:-93518326 None:intergenic
AACGGAAGGAGACACCGAAG+TGG 0.694309 7:+93518520 MsaT039822.1:CDS
GGAATGGGAATATCAGAGCG+AGG 0.695426 7:+93518655 MsaT039822.1:CDS
TACACCAGCTGTGATGCCGG+CGG 0.704142 7:+93518463 MsaT039822.1:CDS
CTTATGCAGATGATGTCCAT+GGG 0.705255 7:+93521367 MsaT039822.1:intron
GCTCCAGAAACCCATAACAC+AGG 0.711170 7:-93522215 None:intergenic
GCAGTGTTCAAAATTGACGA+CGG 0.720480 7:-93518407 None:intergenic
TGCTGCGAGGGAATCAACAG+TGG 0.723697 7:+93518427 MsaT039822.1:CDS
CTGTTGTTATTAACCGTCGG+CGG 0.728995 7:-93518251 None:intergenic
ACGGCCAAGTCGTTACACAG+AGG 0.765940 7:+93518021 MsaT039822.1:CDS
TCCGACGATTCAACTCGCGG+CGG 0.774522 7:+93518086 MsaT039822.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 93517828 93522628 93517828 ID=MsaG039822
Chr7 mRNA 93517828 93522628 93517828 ID=MsaT039822.1;Parent=MsaG039822
Chr7 exon 93517828 93517904 93517828 ID=MsaT039822.1.exon1;Parent=MsaT039822.1
Chr7 CDS 93517828 93517904 93517828 ID=cds.MsaT039822.1;Parent=MsaT039822.1
Chr7 exon 93517986 93518676 93517986 ID=MsaT039822.1.exon2;Parent=MsaT039822.1
Chr7 CDS 93517986 93518676 93517986 ID=cds.MsaT039822.1;Parent=MsaT039822.1
Chr7 exon 93520346 93520441 93520346 ID=MsaT039822.1.exon3;Parent=MsaT039822.1
Chr7 CDS 93520346 93520441 93520346 ID=cds.MsaT039822.1;Parent=MsaT039822.1
Chr7 exon 93520534 93520599 93520534 ID=MsaT039822.1.exon4;Parent=MsaT039822.1
Chr7 CDS 93520534 93520599 93520534 ID=cds.MsaT039822.1;Parent=MsaT039822.1
Chr7 exon 93520710 93520775 93520710 ID=MsaT039822.1.exon5;Parent=MsaT039822.1
Chr7 CDS 93520710 93520775 93520710 ID=cds.MsaT039822.1;Parent=MsaT039822.1
Chr7 exon 93521376 93521750 93521376 ID=MsaT039822.1.exon6;Parent=MsaT039822.1
Chr7 CDS 93521376 93521750 93521376 ID=cds.MsaT039822.1;Parent=MsaT039822.1
Chr7 exon 93522070 93522133 93522070 ID=MsaT039822.1.exon7;Parent=MsaT039822.1
Chr7 CDS 93522070 93522133 93522070 ID=cds.MsaT039822.1;Parent=MsaT039822.1
Chr7 exon 93522208 93522277 93522208 ID=MsaT039822.1.exon8;Parent=MsaT039822.1
Chr7 CDS 93522208 93522277 93522208 ID=cds.MsaT039822.1;Parent=MsaT039822.1
Chr7 exon 93522422 93522503 93522422 ID=MsaT039822.1.exon9;Parent=MsaT039822.1
Chr7 CDS 93522422 93522503 93522422 ID=cds.MsaT039822.1;Parent=MsaT039822.1
Chr7 exon 93522584 93522628 93522584 ID=MsaT039822.1.exon10;Parent=MsaT039822.1
Chr7 CDS 93522584 93522628 93522584 ID=cds.MsaT039822.1;Parent=MsaT039822.1
Gene Sequence

>MsaT039822.1

ATGAACTACTGCGATTCATATTTCGAATCTCAAATGGATCAACAAGCAGGATTACCTCGTCCTAAGAAACCAATCATTGCAGATGATGAGATCATGGAGCTGTTATGGAAAAACGGCCAAGTCGTTACACAGAGGCCAAACCACCGTCATCTGAAGAAACCACTGCCGGTGAAAAACTCCGACGATTCAACTCGCGGCGGAGGAGCTTCATCGGCGAGGGAGATTCGACCAGTAGAAAACTACAATCAATATCTCTTCATGCAAGAAAATGAAATGGCTTCATGGCTTCATTACGATGATTCTCCTTTCGAACAAACCTTTTCCACCGATTTCCTTAACCCGCCGCCGACGGTTAATAACAACAGTACAATACAAACTCCGACGTCGGTGGTTCCTTCTCGTCCTCCAATTCCCCCGCCACGGAGGATAGGGCACGTGCAGCCTGAGACACCGAACTTCGCGTACTTTGCGAAGCACGGTGTAAGAACCGAGCCAGGACCGTCGTCAATTTTGAACACTGCTGCGAGGGAATCAACAGTGGTGGATTCGTGTGATACACCAGCTGTGATGCCGGCGGCGTTTTCGGAGACGGTAAGAAGTTTAGCGGATCAAACGGAAGGAGACACCGAAGTGGCGGTGATTTCGGCAACTTTTGATGAACCGGGCGGTTCAAGTAGCAGTGAAGAAGCTGAACCGGTTGGGAAGGTAGCGGAGCAGGACCGAAAGAGGAAGGGAGTAGAAGCGGAGGAATGGGAATATCAGAGCGAGGATGTTGATTTTGAATCTGCGGAAGCCCAAAAAAATATCAGTGGATCATCTACAAAGAGATCTCGTGCTGCTGAGGTCCATAATCTCTCAGAAAGGAGGCGTCGTGATCGGATTAATGAAAAGATGAAAGCTCTGCAAGAACTTATACCTCGATCTAATAAGTCTGACAAAGCATCTATGCTGGATGAAGCAATTGATTACTTGAAGTCGCTGCAGTTACAAGTGCAGATGATGTCCATGGGATGTGGCATGGTACCTATGATGTTTCCTGGAATCCAGCAGTATATGCCAACAGTTGGAATGGGAATGGGGATGGGTATGGAAATGGGAATAAACAGACCAGTAATGCCATTTCCCAATATGTTATCCGGTTCTGCTTTGCCTGCAGCCGCGAATTTGGGACCAAGGTTCGTGATGCCTCCTTTCCACATGCCGCATGTTCCTACACCTGGTTCATCCAGAATGCAAGCAGCAAATCAGGTAGATAATAATGTGATCACCTCAGCTGGAACACATGATTCAAATCAGTCACGTATCCCAAATTTCAGTGATCCTTATCAACAATATCTTGGACCCCACCAGATGCAGTTTCAATTGATGCAGAATCAAGCAATGAACCAACCAAATGTCAGCAGGCCTCTTGAGAAACCAGAAACCCGCCAGTCAGCTCTTGTCCTGTGTTATGGGTTTCTGGAGCACAGTGACTTCCAGATCTGGTTGTTTACCATGTCAATGAAGGCTGGCTATGCAGATGGTGCTTCTTATTATTGTATATTCTCTTATACTACTAGCTCTCAATATGGTACTACTAGTATTATTGTTATATTTTCTGCCACTCCTAATACAGTATGCCAAGTTTTGTAG
Protein sequence

>MsaT039822.1

MNYCDSYFESQMDQQAGLPRPKKPIIADDEIMELLWKNGQVVTQRPNHRHLKKPLPVKNSDDSTRGGGASSAREIRPVENYNQYLFMQENEMASWLHYDDSPFEQTFSTDFLNPPPTVNNNSTIQTPTSVVPSRPPIPPPRRIGHVQPETPNFAYFAKHGVRTEPGPSSILNTAARESTVVDSCDTPAVMPAAFSETVRSLADQTEGDTEVAVISATFDEPGGSSSSEEAEPVGKVAEQDRKRKGVEAEEWEYQSEDVDFESAEAQKNISGSSTKRSRAAEVHNLSERRRRDRINEKMKALQELIPRSNKSDKASMLDEAIDYLKSLQLQVQMMSMGCGMVPMMFPGIQQYMPTVGMGMGMGMEMGINRPVMPFPNMLSGSALPAAANLGPRFVMPPFHMPHVPTPGSSRMQAANQVDNNVITSAGTHDSNQSRIPNFSDPYQQYLGPHQMQFQLMQNQAMNQPNVSRPLEKPETRQSALVLCYGFLEHSDFQIWLFTMSMKAGYADGASYYCIFSYTTSSQYGTTSIIVIFSATPNTVCQVL*