Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039856 | GAU37750.1 | 39.344 | 183 | 109 | 2 | 29 | 209 | 27 | 209 | 3.74e-36 | 136 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039856 | tr|A0A2Z6NMK1|A0A2Z6NMK1_TRISU | 39.344 | 183 | 109 | 2 | 29 | 209 | 27 | 209 | 1.78e-36 | 136 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG039856 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG039856 | MtrunA17_Chr5g0393501 | 24.157 | 178 | 130 | 3 | 36 | 209 | 66 | 242 | 1.14e-13 | 67.8 |
| MsaG039856 | MtrunA17_Chr1g0175581 | 28.431 | 102 | 73 | 0 | 94 | 195 | 5 | 106 | 9.37e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATGTGATGTACTAATA+AGG | 0.256110 | 7:-93888266 | MsaT039856.1:CDS |
| AATGATCTTTATGAAGTTCT+TGG | 0.265359 | 7:-93888600 | MsaT039856.1:CDS |
| CAAATGTAATGAAGGTTTCT+AGG | 0.290265 | 7:-93888131 | MsaT039856.1:CDS |
| TCATAACCTGCAGCCATATA+AGG | 0.313221 | 7:+93888458 | None:intergenic |
| ATGATCTTTATGAAGTTCTT+GGG | 0.324200 | 7:-93888599 | MsaT039856.1:CDS |
| AATGATCTTAACTCCTTATA+TGG | 0.327626 | 7:-93888471 | MsaT039856.1:CDS |
| CTGAGGGATGGATCAACTTA+AGG | 0.360735 | 7:-93888649 | MsaT039856.1:CDS |
| GTCCACGAAATGCAGTGAAT+TGG | 0.385092 | 7:+93888564 | None:intergenic |
| TAAGCTTAAGTTTAAGTTTG+TGG | 0.399998 | 7:-93888166 | MsaT039856.1:CDS |
| TATGGCTGCAGGTTATGAAT+TGG | 0.411365 | 7:-93888453 | MsaT039856.1:intron |
| GAGTTAAGATCATTTCATAA+TGG | 0.420070 | 7:+93888480 | None:intergenic |
| GGTGATTACATTAGAAGCTC+AGG | 0.426576 | 7:-93888330 | MsaT039856.1:CDS |
| TTCCAATTCACTGCATTTCG+TGG | 0.427022 | 7:-93888566 | MsaT039856.1:CDS |
| CAGACACTGAACATTGACTT+TGG | 0.433491 | 7:-93888351 | MsaT039856.1:CDS |
| TTCTCTGCTATTAATATGTA+TGG | 0.435001 | 7:-93888306 | MsaT039856.1:CDS |
| CAAATGTGATGTACTAATAA+GGG | 0.442101 | 7:-93888265 | MsaT039856.1:CDS |
| TGAGGGATGGATCAACTTAA+GGG | 0.444993 | 7:-93888648 | MsaT039856.1:CDS |
| TTAACTCCTTATATGGCTGC+AGG | 0.483063 | 7:-93888464 | MsaT039856.1:CDS |
| ATTAATATGTATGGACCTAC+AGG | 0.484413 | 7:-93888297 | MsaT039856.1:CDS |
| TGTAAAGACAATGGATTCCA+TGG | 0.503947 | 7:-93888195 | MsaT039856.1:CDS |
| CAACAAAGATTGGCAGTGGA+TGG | 0.512212 | 7:-93888227 | MsaT039856.1:CDS |
| CACTTACAAGCAAGGAATTG+AGG | 0.515012 | 7:-93888510 | MsaT039856.1:CDS |
| AAGTACATCAAATGTAATGA+AGG | 0.524181 | 7:-93888139 | MsaT039856.1:CDS |
| ATGTCAACTCATTGCCATCA+TGG | 0.539340 | 7:-93888535 | MsaT039856.1:CDS |
| ATATCTCATTTACAGCCGTA+TGG | 0.553596 | 7:-93888795 | MsaT039856.1:intron |
| AAGACAATGGATTCCATGGT+GGG | 0.554852 | 7:-93888191 | MsaT039856.1:CDS |
| ATCCATCCCTCAGTTAGAAG+TGG | 0.556411 | 7:+93888659 | None:intergenic |
| ATAAAATGCTCAACAAAGGA+AGG | 0.558305 | 7:+93888762 | None:intergenic |
| TGATCTTTATGAAGTTCTTG+GGG | 0.561506 | 7:-93888598 | MsaT039856.1:CDS |
| AAGGCAACAAAGATTGGCAG+TGG | 0.562007 | 7:-93888231 | MsaT039856.1:CDS |
| TGCTTGTAAGTGTGCCATGA+TGG | 0.567390 | 7:+93888521 | None:intergenic |
| ATCCACTTCTAACTGAGGGA+TGG | 0.574184 | 7:-93888661 | MsaT039856.1:CDS |
| GTTGATAAAATGCTCAACAA+AGG | 0.587741 | 7:+93888758 | None:intergenic |
| CATTTGTACTCGGTGCCTGT+AGG | 0.588159 | 7:+93888282 | None:intergenic |
| TAGTACATCACATTTGTACT+CGG | 0.590498 | 7:+93888272 | None:intergenic |
| TGTGATGTACTAATAAGGGA+TGG | 0.597036 | 7:-93888261 | MsaT039856.1:CDS |
| TACTGCTTATATCATCAATG+AGG | 0.608119 | 7:-93888881 | MsaT039856.1:intron |
| GAAGGTCTAATCTCTCCATA+CGG | 0.613102 | 7:+93888780 | None:intergenic |
| TGAACATCCACTTCTAACTG+AGG | 0.624857 | 7:-93888666 | MsaT039856.1:CDS |
| ACTTAAGCTTATCCCCACCA+TGG | 0.632346 | 7:+93888178 | None:intergenic |
| AAAGACAATGGATTCCATGG+TGG | 0.635851 | 7:-93888192 | MsaT039856.1:CDS |
| AGACAATGGATTCCATGGTG+GGG | 0.640570 | 7:-93888190 | MsaT039856.1:CDS |
| TCATGGCACACTTACAAGCA+AGG | 0.641070 | 7:-93888518 | MsaT039856.1:CDS |
| GAACATCCACTTCTAACTGA+GGG | 0.652020 | 7:-93888665 | MsaT039856.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 93888125 | 93888916 | 93888125 | ID=MsaG039856 |
| Chr7 | mRNA | 93888125 | 93888916 | 93888125 | ID=MsaT039856.1;Parent=MsaG039856 |
| Chr7 | exon | 93888125 | 93888370 | 93888125 | ID=MsaT039856.1.exon3;Parent=MsaT039856.1 |
| Chr7 | CDS | 93888125 | 93888370 | 93888125 | ID=cds.MsaT039856.1;Parent=MsaT039856.1 |
| Chr7 | exon | 93888454 | 93888802 | 93888454 | ID=MsaT039856.1.exon2;Parent=MsaT039856.1 |
| Chr7 | CDS | 93888454 | 93888802 | 93888454 | ID=cds.MsaT039856.1;Parent=MsaT039856.1 |
| Chr7 | exon | 93888882 | 93888916 | 93888882 | ID=MsaT039856.1.exon1;Parent=MsaT039856.1 |
| Chr7 | CDS | 93888882 | 93888916 | 93888882 | ID=cds.MsaT039856.1;Parent=MsaT039856.1 |
| Gene Sequence |
| Protein sequence |
