AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG039860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039860 MtrunA17_Chr7g0263121 79.032 186 16 2 3 179 1 172 2.06e-98 281
MsaG039860 MtrunA17_Chr7g0263131 71.839 174 30 4 1 173 1 156 1.29e-81 239
MsaG039860 MtrunA17_Chr1g0182181 58.621 174 52 3 9 176 3 162 4.31e-63 192
MsaG039860 MtrunA17_Chr1g0182161 57.838 185 48 7 3 176 2 167 1.07e-58 181
MsaG039860 MtrunA17_Chr1g0212561 39.623 212 74 9 3 175 1 197 5.88e-33 116
MsaG039860 MtrunA17_Chr2g0327901 45.570 158 49 6 11 164 4 128 3.19e-29 104
MsaG039860 MtrunA17_Chr7g0222831 46.512 129 42 4 3 123 1 110 3.76e-27 101
MsaG039860 MtrunA17_Chr1g0197281 40.397 151 64 4 37 163 156 304 4.39e-24 96.7
MsaG039860 MtrunA17_Chr2g0320311 35.484 217 78 8 10 165 68 283 1.75e-21 89.0
MsaG039860 MtrunA17_Chr1g0180311 40.136 147 52 4 16 126 80 226 6.17e-21 87.4
MsaG039860 MtrunA17_Chr4g0027991 34.545 165 62 4 37 165 113 267 2.38e-20 85.5
MsaG039860 MtrunA17_Chr7g0231181 35.862 145 63 6 22 163 225 342 2.16e-17 78.2
MsaG039860 MtrunA17_Chr4g0012691 38.636 132 65 4 35 157 218 342 4.54e-17 77.4
MsaG039860 MtrunA17_Chr1g0152661 45.882 85 40 1 37 121 81 159 2.89e-16 73.6
MsaG039860 MtrunA17_Chr4g0012791 33.129 163 79 3 30 162 220 382 6.30e-16 74.7
MsaG039860 MtrunA17_Chr1g0207451 40.323 124 54 3 4 121 44 153 7.01e-16 72.8
MsaG039860 MtrunA17_Chr1g0188911 33.333 162 53 6 37 157 44 191 1.61e-14 68.2
MsaG039860 MtrunA17_Chr5g0425411 45.745 94 38 4 36 121 118 206 1.31e-13 66.6
MsaG039860 MtrunA17_Chr1g0188901 37.879 132 63 5 37 157 60 183 2.03e-13 65.1
MsaG039860 MtrunA17_Chr3g0134711 43.529 85 42 2 37 121 86 164 1.12e-12 63.9
MsaG039860 MtrunA17_Chr5g0448611 36.111 108 50 3 33 121 126 233 2.82e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039860 AT2G37430.1 44.444 189 76 5 3 177 1 174 1.58e-46 150
MsaG039860 AT3G53600.1 43.684 190 73 6 3 177 1 171 1.39e-41 138
MsaG039860 AT2G28710.1 44.828 174 71 5 3 171 1 154 2.14e-35 121
MsaG039860 AT5G59820.1 39.655 174 60 7 15 170 13 159 1.50e-26 99.4
MsaG039860 AT2G28200.1 38.554 166 72 7 28 165 103 266 1.61e-26 102
MsaG039860 AT3G46090.1 42.442 172 67 7 12 170 11 163 1.67e-25 96.7
MsaG039860 AT5G03510.1 38.342 193 77 7 13 163 69 261 5.44e-23 93.2
MsaG039860 AT3G10470.1 50.485 103 34 4 37 122 182 284 1.78e-22 93.2
MsaG039860 AT3G46080.1 40.491 163 70 5 18 170 16 161 2.95e-22 88.2
MsaG039860 AT5G04390.1 33.918 171 72 4 37 166 153 323 2.21e-21 89.7
MsaG039860 AT3G46070.1 36.145 166 61 5 18 162 16 157 2.40e-21 85.9
MsaG039860 AT3G60580.1 37.500 136 64 6 35 168 171 287 1.48e-18 81.3
MsaG039860 AT1G02030.1 45.918 98 40 3 30 127 153 237 1.56e-17 78.2
MsaG039860 AT4G35280.1 37.975 158 72 7 13 157 138 282 8.37e-17 76.3
MsaG039860 AT5G56200.1 41.000 100 52 1 39 131 344 443 1.37e-16 77.0
MsaG039860 AT2G45120.1 38.000 150 65 7 29 171 186 314 2.96e-16 75.1
MsaG039860 AT2G17180.1 32.886 149 95 3 13 157 121 268 7.49e-16 73.6
MsaG039860 AT5G43170.1 41.284 109 40 4 18 121 51 140 4.06e-14 67.8
MsaG039860 AT1G49900.1 44.318 88 33 3 37 121 193 267 1.51e-13 68.2
MsaG039860 AT1G49900.1 37.647 85 39 1 37 121 750 820 4.36e-12 63.9
MsaG039860 AT1G27730.1 37.692 130 60 5 4 121 38 158 1.53e-13 66.6
MsaG039860 AT5G04340.1 43.478 92 35 3 37 121 89 170 2.09e-13 66.6
MsaG039860 AT5G67450.1 39.560 91 50 2 36 121 96 186 6.18e-13 65.1
MsaG039860 AT3G19580.1 40.000 95 42 3 33 121 102 187 9.60e-13 65.1
MsaG039860 AT3G19580.2 40.000 95 42 3 33 121 102 187 9.60e-13 65.1
MsaG039860 AT3G49930.1 38.202 89 42 2 36 121 93 171 1.12e-12 63.9
MsaG039860 AT1G02040.1 34.043 141 61 3 13 121 107 247 3.93e-11 60.8

Find 28 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTAGTTTCATTCTCTTA+TGG 0.261088 7:+93935327 None:intergenic
TTCTCTTATGGCTAGCTCTA+TGG 0.296077 7:+93935339 None:intergenic
AATGATTTGAAGTTGTTGTT+TGG 0.347319 7:-93930847 MsaT039860.1:CDS
CTATTCAATCTCTTTAAAAC+TGG 0.356656 7:+93930913 None:intergenic
CAACAGAAAAGCTATGAAAA+TGG 0.359512 7:-93935419 MsaT039860.1:CDS
CATAAAAGATCAAAGCTAGA+AGG 0.385523 7:-93931114 MsaT039860.1:CDS
TTCATAACAACTTGCTCCAT+TGG 0.405809 7:+93930940 None:intergenic
AATTCTACATCTTTGAGCTT+AGG 0.417627 7:-93931075 MsaT039860.1:CDS
GAAGTTGTTGTTTGGAAAGA+TGG 0.423340 7:-93930839 MsaT039860.1:CDS
CAAACTAAAACATCAAGATT+AGG 0.441068 7:+93930814 None:intergenic
GGACTTGAACTTGACACCAT+TGG 0.443698 7:-93930872 MsaT039860.1:CDS
AAGAGACAAAGGAGCAATGA+AGG 0.461364 7:-93935491 MsaT039860.1:CDS
TGCATGAATGTTCCATTTGT+GGG 0.475350 7:-93931038 MsaT039860.1:CDS
ATGCATGAATGTTCCATTTG+TGG 0.513081 7:-93931039 MsaT039860.1:CDS
TAAAGAGATTGAATAGTGCA+AGG 0.521881 7:-93930906 MsaT039860.1:CDS
CAACGAAGAGGTTTCTCCAA+TGG 0.523977 7:-93930956 MsaT039860.1:CDS
GGCTAGCTCTATGGCCACCA+AGG 0.538489 7:+93935348 None:intergenic
ATAAAAGATCAAAGCTAGAA+GGG 0.549441 7:-93931113 MsaT039860.1:CDS
AAAGAGATTGAATAGTGCAA+GGG 0.553931 7:-93930905 MsaT039860.1:CDS
TAGTGCAAGGGTAATGTGCT+TGG 0.582174 7:-93930893 MsaT039860.1:CDS
TAAAGCTATTATCAACGAAG+AGG 0.599520 7:-93930968 MsaT039860.1:CDS
GCTAGCTCTATGGCCACCAA+GGG 0.600232 7:+93935349 None:intergenic
AACTTCAAATCATTCTCCAA+TGG 0.606116 7:+93930856 None:intergenic
CATTGGGTGGACACATGAGA+AGG 0.615753 7:-93930993 MsaT039860.1:CDS
TCATTGGGACAAGCATTGGG+TGG 0.622140 7:-93931006 MsaT039860.1:CDS
TGATGGCAATGAAGAGACAA+AGG 0.625383 7:-93935502 MsaT039860.1:CDS
TAAAAGATCAAAGCTAGAAG+GGG 0.632211 7:-93931112 MsaT039860.1:CDS
AAGAGAATGAAACTAGCTGA+AGG 0.634408 7:-93935323 MsaT039860.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 93930813 93935522 93930813 ID=MsaG039860
Chr7 mRNA 93930813 93935522 93930813 ID=MsaT039860.1;Parent=MsaG039860
Chr7 exon 93930813 93931153 93930813 ID=MsaT039860.1.exon2;Parent=MsaT039860.1
Chr7 CDS 93930813 93931153 93930813 ID=cds.MsaT039860.1;Parent=MsaT039860.1
Chr7 exon 93935324 93935522 93935324 ID=MsaT039860.1.exon1;Parent=MsaT039860.1
Chr7 CDS 93935324 93935522 93935324 ID=cds.MsaT039860.1;Parent=MsaT039860.1
Gene Sequence

>MsaT039860.1

ATGGCAATGAAGAGACAAAGGAGCAATGAAGGAGCAGAGAGCATAGATTATGCAAATTGTTTGATGCTACTATCTTGTCCACAACAGAAAAGCTATGAAAATGGTGAATATGAATGCAAAACATGCAACAAAAAATTCTCATCTTTTCAAGCCCTTGGTGGCCATAGAGCTAGCCATAAGAGAATGAAACTAGCTGAAGGTGGCCACAGAGCTAGCCATAAAAGATCAAAGCTAGAAGGGGATGAATTACTAACAAATTCTACATCTTTGAGCTTAGGAAATACGCCTAAAATGCATGAATGTTCCATTTGTGGGCAAAAATTTTCATTGGGACAAGCATTGGGTGGACACATGAGAAGGCATAAAGCTATTATCAACGAAGAGGTTTCTCCAATGGAGCAAGTTGTTATGAAATTACCAGTTTTAAAGAGATTGAATAGTGCAAGGGTAATGTGCTTGGACTTGAACTTGACACCATTGGAGAATGATTTGAAGTTGTTGTTTGGAAAGATGGCTCCTAATCTTGATGTTTTAGTTTGA
Protein sequence

>MsaT039860.1

MAMKRQRSNEGAESIDYANCLMLLSCPQQKSYENGEYECKTCNKKFSSFQALGGHRASHKRMKLAEGGHRASHKRSKLEGDELLTNSTSLSLGNTPKMHECSICGQKFSLGQALGGHMRRHKAIINEEVSPMEQVVMKLPVLKRLNSARVMCLDLNLTPLENDLKLLFGKMAPNLDVLV*