AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG039926

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039926 MtrunA17_Chr7g0263941 95.332 407 17 2 1 406 1 406 0.0 789
MsaG039926 MtrunA17_Chr1g0181381 58.045 491 98 13 3 387 4 492 3.58e-165 473
MsaG039926 MtrunA17_Chr2g0330171 63.251 283 84 4 33 302 45 320 1.47e-118 353
MsaG039926 MtrunA17_Chr4g0029521 73.756 221 45 6 7 218 6 222 4.16e-115 345
MsaG039926 MtrunA17_Chr8g0342421 57.402 331 102 8 27 329 55 374 3.08e-114 343
MsaG039926 MtrunA17_Chr1g0199101 87.500 168 20 1 34 200 47 214 4.79e-112 338
MsaG039926 MtrunA17_Chr1g0191991 56.369 314 111 4 34 328 63 369 8.64e-112 337
MsaG039926 MtrunA17_Chr4g0040571 83.051 177 29 1 28 204 34 209 2.78e-111 335
MsaG039926 MtrunA17_Chr1g0163001 82.967 182 27 2 23 204 34 211 3.61e-111 336
MsaG039926 MtrunA17_Chr1g0185121 54.942 344 107 9 30 340 36 364 1.81e-110 331
MsaG039926 MtrunA17_Chr1g0162971 82.941 170 29 0 34 203 41 210 8.55e-110 330
MsaG039926 MtrunA17_Chr8g0373541 73.333 210 50 2 11 220 15 218 1.32e-109 332
MsaG039926 MtrunA17_Chr1g0151351 62.191 283 84 9 30 308 38 301 8.48e-109 327
MsaG039926 MtrunA17_Chr1g0210821 86.875 160 21 0 37 196 47 206 5.03e-108 329
MsaG039926 MtrunA17_Chr7g0268961 52.113 355 125 9 44 365 32 374 3.49e-105 319
MsaG039926 MtrunA17_Chr2g0326381 62.403 258 74 8 34 283 32 274 7.79e-105 316
MsaG039926 MtrunA17_Chr3g0141431 46.212 396 156 12 21 373 6 387 3.51e-99 301
MsaG039926 MtrunA17_Chr2g0324991 87.023 131 17 0 45 175 3 133 3.70e-85 256
MsaG039926 MtrunA17_Chr6g0479921 64.557 158 55 1 32 188 27 184 2.77e-74 239
MsaG039926 MtrunA17_Chr8g0375521 64.103 156 54 2 34 187 27 182 4.11e-72 232
MsaG039926 MtrunA17_Chr3g0123941 37.821 156 89 3 39 186 113 268 4.36e-26 107
MsaG039926 MtrunA17_Chr1g0200421 35.762 151 85 4 48 186 236 386 1.09e-24 105
MsaG039926 MtrunA17_Chr2g0320341 34.839 155 87 4 43 183 224 378 1.45e-23 101
MsaG039926 MtrunA17_Chr8g0382121 35.948 153 87 5 42 183 104 256 3.81e-22 96.3
MsaG039926 MtrunA17_Chr5g0422571 34.254 181 102 6 21 191 78 251 9.68e-22 94.7
MsaG039926 MtrunA17_Chr1g0198651 34.503 171 95 5 28 186 84 249 5.69e-21 92.4
MsaG039926 MtrunA17_Chr4g0053341 35.762 151 87 5 45 186 69 218 2.24e-20 89.4
MsaG039926 MtrunA17_Chr4g0026521 36.301 146 83 5 50 186 118 262 2.41e-19 87.8
MsaG039926 MtrunA17_Chr8g0352011 36.301 146 83 5 50 186 166 310 3.34e-19 88.2
MsaG039926 MtrunA17_Chr2g0321291 34.054 185 103 8 50 223 133 309 3.52e-19 87.4
MsaG039926 MtrunA17_Chr7g0258011 34.932 146 85 5 50 186 176 320 6.75e-19 87.4
MsaG039926 MtrunA17_Chr1g0173841 34.932 146 85 5 50 186 215 359 2.06e-18 86.3
MsaG039926 MtrunA17_Chr2g0328921 34.932 146 85 5 50 186 138 282 2.86e-18 85.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG039926 AT5G03150.1 61.489 309 71 9 3 283 4 292 1.78e-117 352
MsaG039926 AT3G50700.1 85.549 173 25 0 24 196 24 196 2.26e-113 339
MsaG039926 AT1G55110.3 62.799 293 84 8 30 306 58 341 1.64e-111 335
MsaG039926 AT1G55110.1 62.799 293 84 8 30 306 58 341 1.64e-111 335
MsaG039926 AT1G55110.2 62.799 293 84 8 30 306 58 341 1.64e-111 335
MsaG039926 AT5G66730.1 75.728 206 43 2 11 209 8 213 1.68e-111 336
MsaG039926 AT5G44160.1 52.676 355 109 8 28 329 32 380 5.48e-111 333
MsaG039926 AT3G45260.2 84.181 177 23 2 33 204 38 214 1.55e-108 327
MsaG039926 AT3G45260.1 84.181 177 23 2 33 204 38 214 1.55e-108 327
MsaG039926 AT1G03840.1 78.610 187 38 2 28 213 36 221 2.12e-108 328
MsaG039926 AT3G13810.6 86.826 167 21 1 31 196 48 214 4.24e-106 322
MsaG039926 AT3G13810.4 86.826 167 21 1 31 196 67 233 4.40e-106 323
MsaG039926 AT3G13810.1 86.826 167 21 1 31 196 67 233 4.40e-106 323
MsaG039926 AT3G13810.5 86.826 167 21 1 31 196 62 228 5.55e-106 322
MsaG039926 AT1G14580.1 82.738 168 26 1 29 196 51 215 6.51e-106 321
MsaG039926 AT1G14580.2 82.738 168 26 1 29 196 51 215 6.51e-106 321
MsaG039926 AT2G02070.2 83.529 170 26 1 34 203 52 219 8.33e-106 325
MsaG039926 AT2G02070.1 83.529 170 26 1 34 203 52 219 8.33e-106 325
MsaG039926 AT1G14580.3 82.738 168 26 1 29 196 65 229 8.41e-106 321
MsaG039926 AT1G03840.2 77.540 187 38 3 28 213 36 219 9.52e-105 319
MsaG039926 AT2G02080.1 70.647 201 46 3 7 197 20 217 1.10e-104 319
MsaG039926 AT2G02080.4 70.647 201 46 3 7 197 20 217 1.10e-104 319
MsaG039926 AT2G02080.5 70.647 201 46 3 7 197 20 217 1.10e-104 319
MsaG039926 AT2G02080.3 70.647 201 46 3 7 197 20 217 1.10e-104 319
MsaG039926 AT4G02670.1 81.714 175 31 1 34 207 53 227 1.16e-104 315
MsaG039926 AT4G02670.2 81.714 175 31 1 34 207 55 229 1.38e-104 315
MsaG039926 AT5G60470.3 75.521 192 38 3 21 203 20 211 7.47e-104 315
MsaG039926 AT5G60470.2 75.521 192 38 3 21 203 24 215 8.42e-104 315
MsaG039926 AT5G44160.2 51.622 339 106 7 44 329 8 341 6.73e-103 311
MsaG039926 AT3G13810.3 88.235 153 17 1 45 196 68 220 8.97e-98 301
MsaG039926 AT3G13810.2 88.235 153 17 1 45 196 82 234 1.03e-97 301
MsaG039926 AT5G60470.1 80.745 161 29 2 45 203 46 206 1.27e-94 291
MsaG039926 AT2G02080.6 81.295 139 26 0 58 196 1 139 3.05e-88 274
MsaG039926 AT2G02080.2 81.295 139 26 0 58 196 1 139 3.05e-88 274
MsaG039926 AT1G68130.1 56.842 190 74 3 32 219 39 222 1.42e-74 238
MsaG039926 AT1G25250.3 64.780 159 53 2 32 187 31 189 6.20e-74 236
MsaG039926 AT2G01940.1 63.190 163 58 2 32 192 42 204 1.43e-73 237
MsaG039926 AT2G01940.3 62.195 164 59 3 32 192 42 205 6.04e-70 227
MsaG039926 AT1G25250.1 65.306 147 48 2 44 187 20 166 4.91e-69 222
MsaG039926 AT1G68130.2 52.817 142 59 3 80 219 1 136 1.35e-45 160
MsaG039926 AT2G01940.2 60.870 115 43 2 80 192 1 115 3.57e-44 157
MsaG039926 AT1G25250.2 62.162 111 39 2 80 187 1 111 3.91e-44 155
MsaG039926 AT1G34370.3 29.918 244 124 8 43 244 14 252 1.48e-24 102
MsaG039926 AT1G34370.2 29.918 244 124 8 43 244 224 462 4.47e-24 104
MsaG039926 AT1G34370.1 29.918 244 124 8 43 244 224 462 4.47e-24 104
MsaG039926 AT5G22890.1 32.530 166 96 4 35 186 191 354 1.47e-22 98.6
MsaG039926 AT1G13290.1 34.211 152 88 5 55 195 93 243 2.10e-19 88.6
MsaG039926 AT1G08290.1 35.849 159 85 7 44 186 155 312 1.51e-18 86.7
MsaG039926 AT1G34790.1 33.333 180 104 7 47 218 129 300 1.55e-18 85.9
MsaG039926 AT3G20880.1 34.247 146 86 5 50 186 243 387 3.38e-18 86.3
MsaG039926 AT1G51220.1 33.562 146 87 5 50 186 165 309 5.42e-18 84.7
MsaG039926 AT3G57670.1 34.247 146 86 5 50 186 204 348 6.88e-18 85.1

Find 79 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTGGTTTGTGGTGGATT+AGG 0.210609 7:-94539177 None:intergenic
CCATGATGCGGCCAGGGCCT+TGG 0.232187 7:+94539541 MsaT039926.1:CDS
AGATTGAACTGCATATTTCT+TGG 0.249648 7:-94539636 None:intergenic
TGCTCACAAATGAATATTCT+TGG 0.256662 7:+94539090 None:intergenic
GCTAAAATGTTTCTTTATTC+CGG 0.260327 7:-94539573 None:intergenic
TAGATTTCTCTTCTTCTTGC+TGG 0.261742 7:-94539193 None:intergenic
CTTCAGAAAGCAGCTCAAAT+TGG 0.266190 7:+94540800 MsaT039926.1:CDS
ACTCATCATCGATGAACTTA+TGG 0.281450 7:-94541083 None:intergenic
TGCTGGTTTGTGGTGGATTA+GGG 0.287287 7:-94539176 None:intergenic
TATTAACCTCATCATGGTTT+TGG 0.287752 7:-94540910 None:intergenic
TTCGTCGCCATTAGAGTCTT+TGG 0.293737 7:-94539362 None:intergenic
GCTGAAGATTCTGATCTCTT+TGG 0.295858 7:-94539418 None:intergenic
GTTTGTGGTGGATTAGGGTT+AGG 0.305769 7:-94539171 None:intergenic
CAACAAAGAACCAAGGAAAA+AGG 0.327722 7:+94539487 MsaT039926.1:CDS
CTCTTCTTCTTGCTGGTTTG+TGG 0.328936 7:-94539186 None:intergenic
TATTTGACGACTCTTCATTC+TGG 0.329154 7:-94540538 None:intergenic
TTAATATTACCTGGTGTTCC+TGG 0.358554 7:-94539216 None:intergenic
GAAAACATGGTGAGAAGAAA+TGG 0.363622 7:+94539597 MsaT039926.1:CDS
AAACCATGATGAGGTTAATA+TGG 0.368833 7:+94540913 MsaT039926.1:CDS
CATGATGCGGCCAGGGCCTT+GGG 0.370145 7:+94539542 MsaT039926.1:CDS
TTCTGGAAATTTAGAGTTGT+AGG 0.385136 7:-94540521 None:intergenic
GCTCTTGAGGTAGAAATTGA+TGG 0.404206 7:-94541045 None:intergenic
GTGTCCACCATGATGCGGCC+AGG 0.413040 7:+94539534 MsaT039926.1:CDS
TCTTTCTCCAAAGACTCTAA+TGG 0.414643 7:+94539355 MsaT039926.1:CDS
TGCATATTTCGCAGACGAAT+CGG 0.417602 7:-94539385 None:intergenic
TCACTTGATCATGTTTCATC+AGG 0.423280 7:+94540719 MsaT039926.1:CDS
TTTGTGGTGGATTAGGGTTA+GGG 0.429876 7:-94539170 None:intergenic
AATATGCAGTTCAATCTGAT+TGG 0.433651 7:+94539642 MsaT039926.1:CDS
TGGTGGACACAGGTCTTCTC+CGG 0.437364 7:-94539521 None:intergenic
TTCAACCACAGTGACAAGTT+TGG 0.441620 7:-94540662 None:intergenic
GACCTTTGCTTTAGCTTCCA+TGG 0.442243 7:-94539464 None:intergenic
AGTTTAAATCAATTCAATGC+TGG 0.449709 7:+94541103 MsaT039926.1:CDS
TTGAAGTTTGTCAAAACCTC+CGG 0.458884 7:-94539139 None:intergenic
GGTGAGGTCACCCAAGGCCC+TGG 0.470064 7:-94539552 None:intergenic
GTGGAAGAGGATAGTGAAAA+GGG 0.477404 7:-94539117 None:intergenic
TTCTTCTTGCTGGTTTGTGG+TGG 0.478291 7:-94539183 None:intergenic
CTATGGTTGGTAATTCTAGT+GGG 0.487415 7:+94540996 MsaT039926.1:CDS
GTTTAAATCAATTCAATGCT+GGG 0.488084 7:+94541104 MsaT039926.1:CDS
TTTGCTTTAGCTTCCATGGT+AGG 0.488944 7:-94539460 None:intergenic
GTCAAAACCTCCGGTGGAAG+AGG 0.490780 7:-94539130 None:intergenic
AGGGCCTTGGGTGACCTCAC+CGG 0.495108 7:+94539554 MsaT039926.1:CDS
TGGTAGTACTAGTGCAGCTA+TGG 0.495591 7:+94540979 MsaT039926.1:CDS
CCAAGGCCCTGGCCGCATCA+TGG 0.496782 7:-94539541 None:intergenic
TGTCCACCATGATGCGGCCA+GGG 0.498285 7:+94539535 MsaT039926.1:CDS
GCAAACTTTGCTAGCTCTTG+AGG 0.498977 7:-94541058 None:intergenic
GCTATGGTTGGTAATTCTAG+TGG 0.507355 7:+94540995 MsaT039926.1:CDS
GGTGGAAGAGGATAGTGAAA+AGG 0.509534 7:-94539118 None:intergenic
AGAGCAATGACTTCAGCATC+CGG 0.519151 7:-94539335 None:intergenic
TGACCATATTAACCTCATCA+TGG 0.523171 7:-94540916 None:intergenic
CAACAATTTAGTCCACATGC+AGG 0.543450 7:+94540623 MsaT039926.1:CDS
TAAACTCGTTGTTTGTGTAG+AGG 0.545158 7:-94540885 None:intergenic
GGCCGCATCATGGTGGACAC+AGG 0.576015 7:-94539531 None:intergenic
AATCTTCAGCTTCATAGAAG+AGG 0.576550 7:+94539431 MsaT039926.1:CDS
AGTACTAGTGCAGCTATGGT+TGG 0.589645 7:+94540983 MsaT039926.1:CDS
TATTCCGGTGAGGTCACCCA+AGG 0.589649 7:-94539558 None:intergenic
GGTGGACACAGGTCTTCTCC+GGG 0.591060 7:-94539520 None:intergenic
AGAAATCTACCAGGAACACC+AGG 0.591098 7:+94539207 MsaT039926.1:CDS
GAAACATGATCAAGTGAATG+TGG 0.592443 7:-94540713 None:intergenic
TGCTCATGAAATCCTGCATG+TGG 0.593771 7:-94540635 None:intergenic
ACTGTGGTACACTCTTCTCT+AGG 0.600160 7:+94539708 MsaT039926.1:CDS
TCACTATCCTCTTCCACCGG+AGG 0.603318 7:+94539123 MsaT039926.1:CDS
GAAGAGGTCACAACCTACCA+TGG 0.609397 7:+94539447 MsaT039926.1:CDS
TTTAAACCAAAACCATGATG+AGG 0.609478 7:+94540904 MsaT039926.1:CDS
GTCTGCGAAATATGCAACAA+AGG 0.612364 7:+94539392 MsaT039926.1:CDS
AGAAGTGCAGTAGCTGACAT+TGG 0.615729 7:-94540779 None:intergenic
TACCATGGAAGCTAAAGCAA+AGG 0.618651 7:+94539462 MsaT039926.1:CDS
AATGTTTCTTTATTCCGGTG+AGG 0.626985 7:-94539568 None:intergenic
CATGGATTGATATCATCTCA+AGG 0.627308 7:+94540587 MsaT039926.1:CDS
AAGTTTGTCAAAACCTCCGG+TGG 0.633133 7:-94539136 None:intergenic
GACCTGTGTCCACCATGATG+CGG 0.639865 7:+94539529 MsaT039926.1:CDS
GCTGCACTAGTACTACCACT+TGG 0.640622 7:-94540974 None:intergenic
ACACAAACTAGTTTGTCACA+TGG 0.646370 7:+94540569 MsaT039926.1:CDS
AAAGCTCACACTAAAACTTG+TGG 0.646859 7:+94539665 MsaT039926.1:CDS
AAAGGTCCAACAAAGAACCA+AGG 0.658252 7:+94539480 MsaT039926.1:CDS
AAGAAGAAGAGAAATCTACC+AGG 0.661464 7:+94539198 MsaT039926.1:CDS
AGGCCCTGGCCGCATCATGG+TGG 0.664444 7:-94539538 None:intergenic
AGAGAATATAAGTGTGACTG+TGG 0.678389 7:+94539692 MsaT039926.1:CDS
TGGAAGAGGATAGTGAAAAG+GGG 0.698551 7:-94539116 None:intergenic
AAAGACCAAACTTGTCACTG+TGG 0.746204 7:+94540657 MsaT039926.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 94539099 94541144 94539099 ID=MsaG039926
Chr7 mRNA 94539099 94541144 94539099 ID=MsaT039926.1;Parent=MsaG039926
Chr7 exon 94539099 94539228 94539099 ID=MsaT039926.1.exon1;Parent=MsaT039926.1
Chr7 CDS 94539099 94539228 94539099 ID=cds.MsaT039926.1;Parent=MsaT039926.1
Chr7 exon 94539333 94539729 94539333 ID=MsaT039926.1.exon2;Parent=MsaT039926.1
Chr7 CDS 94539333 94539729 94539333 ID=cds.MsaT039926.1;Parent=MsaT039926.1
Chr7 exon 94540451 94541144 94540451 ID=MsaT039926.1.exon3;Parent=MsaT039926.1
Chr7 CDS 94540451 94541144 94540451 ID=cds.MsaT039926.1;Parent=MsaT039926.1
Gene Sequence

>MsaT039926.1

ATGAATATTCTTGGTGACCCCTTTTCACTATCCTCTTCCACCGGAGGTTTTGACAAACTTCAAAACACAAACCCTAACCCTAATCCACCACAAACCAGCAAGAAGAAGAGAAATCTACCAGGAACACCAGATCCGGATGCTGAAGTCATTGCTCTTTCTCCAAAGACTCTAATGGCGACGAACCGATTCGTCTGCGAAATATGCAACAAAGGATTCCAAAGAGATCAGAATCTTCAGCTTCATAGAAGAGGTCACAACCTACCATGGAAGCTAAAGCAAAGGTCCAACAAAGAACCAAGGAAAAAGGTATACATCTGCCCGGAGAAGACCTGTGTCCACCATGATGCGGCCAGGGCCTTGGGTGACCTCACCGGAATAAAGAAACATTTTAGCAGAAAACATGGTGAGAAGAAATGGAAGTGTGATAAGTGTTCCAAGAAATATGCAGTTCAATCTGATTGGAAAGCTCACACTAAAACTTGTGGTACTAGAGAATATAAGTGTGACTGTGGTACACTCTTCTCTAGGAAGGATAGCTTTATTACACATAGAGCTTTCTGTGACGCATTAGCTGTAGAAAGTGCAAGACACTCATCTCCTACAACTCTAAATTTCCAGAATGAAGAGTCGTCAAATATGATGAACACACAAACTAGTTTGTCACATGGATTGATATCATCTCAAGGACTTCAAAGTCTTCAACAATTTAGTCCACATGCAGGATTTCATGAGCAAAGACCAAACTTGTCACTGTGGTTGAACCAAGAAAATCAACAGATTAATCATCATCCACATTCACTTGATCATGTTTCATCAGGTTTTTCTGATGTTGTGCAAATGGCTCAAACTAATACTCCAATGTCAGCTACTGCACTTCTTCAGAAAGCAGCTCAAATTGGTTCAACAAGAAGCACTACTAATCCTTCCATTTTTAGTGGAAGTTTTGGTGTAATGAGTTCATCCTCTACACAAACAACGAGTTTAAACCAAAACCATGATGAGGTTAATATGGTCATTAATCAGAAAATGAAGCAGCATGAGAATTTTGATCCAAGTGGTAGTACTAGTGCAGCTATGGTTGGTAATTCTAGTGGGTTTAGAGATTTTCTTGGAGTGAGTAACCATCAATTTCTACCTCAAGAGCTAGCAAAGTTTGCTTCCATAAGTTCATCGATGATGAGTTTAAATCAATTCAATGCTGGGTGCATCAATGAAAATTAA
Protein sequence

>MsaT039926.1

MNILGDPFSLSSSTGGFDKLQNTNPNPNPPQTSKKKRNLPGTPDPDAEVIALSPKTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRSNKEPRKKVYICPEKTCVHHDAARALGDLTGIKKHFSRKHGEKKWKCDKCSKKYAVQSDWKAHTKTCGTREYKCDCGTLFSRKDSFITHRAFCDALAVESARHSSPTTLNFQNEESSNMMNTQTSLSHGLISSQGLQSLQQFSPHAGFHEQRPNLSLWLNQENQQINHHPHSLDHVSSGFSDVVQMAQTNTPMSATALLQKAAQIGSTRSTTNPSIFSGSFGVMSSSSTQTTSLNQNHDEVNMVINQKMKQHENFDPSGSTSAAMVGNSSGFRDFLGVSNHQFLPQELAKFASISSSMMSLNQFNAGCINEN*