AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040058


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040058 MtrunA17_Chr7g0265561 97.585 414 8 2 1 412 42 455 0.0 821
MsaG040058 MtrunA17_Chr1g0179561 61.949 431 134 8 8 412 70 496 3.92e-150 434
MsaG040058 MtrunA17_Chr2g0328901 40.318 377 175 8 32 399 80 415 3.21e-78 248
MsaG040058 MtrunA17_Chr1g0201951 39.524 420 198 11 8 398 55 447 4.64e-77 246
MsaG040058 MtrunA17_Chr6g0463911 38.200 411 211 9 8 396 53 442 1.10e-73 237
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040058 AT3G10040.1 45.763 413 169 10 6 402 45 418 8.55e-100 304
MsaG040058 AT1G21200.1 57.407 162 61 3 32 188 84 242 1.33e-54 187
MsaG040058 AT1G21200.2 57.407 162 61 3 32 188 84 242 1.33e-54 187
MsaG040058 AT1G76870.2 50.273 183 81 4 7 188 34 207 1.33e-54 186
MsaG040058 AT1G76870.1 50.273 183 81 4 7 188 34 207 1.33e-54 186
MsaG040058 AT1G76870.3 50.273 183 81 4 7 188 34 207 1.33e-54 186

Find 75 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAACTTTGTTGGATACTA+TGG 0.197558 7:+96198616 MsaT040058.1:CDS
TCCAAAGGGATGATGGAAAA+AGG 0.229973 7:+96198482 MsaT040058.1:CDS
TGGTTTGTTGTTGTTGGTTT+TGG 0.247414 7:-96198216 None:intergenic
TGAAAATGAAAGGAAGAGTT+TGG 0.281655 7:+96199285 MsaT040058.1:CDS
AAAAGAGTTAATGATATTCT+TGG 0.286176 7:+96198563 MsaT040058.1:CDS
TCAAATGGGGTAGGGAGTTT+AGG 0.306322 7:+96198848 MsaT040058.1:CDS
AAGAAGAGGGTGGTGCAATT+AGG 0.323802 7:+96199154 MsaT040058.1:CDS
CAATGTTTGCATTCATCAAA+TGG 0.340659 7:+96198833 MsaT040058.1:CDS
GATGAAGATGAACCATGTTT+TGG 0.349130 7:+96198263 MsaT040058.1:CDS
TCAGGAGAAGGTGGTGGTTA+TGG 0.365147 7:+96198971 MsaT040058.1:CDS
AGCTTCTTCTTTCAACTTTG+CGG 0.379123 7:-96198645 None:intergenic
AATGTTTGCATTCATCAAAT+GGG 0.387098 7:+96198834 MsaT040058.1:CDS
AATGGTAAGACTCTTAATAA+TGG 0.395318 7:+96198358 MsaT040058.1:CDS
TGTTGAAAATCAAACTTTGT+TGG 0.398526 7:+96198607 MsaT040058.1:CDS
GAGTTTGGAGATTGATAGAA+TGG 0.398897 7:+96199300 MsaT040058.1:CDS
ATGAAAATATTGATCACTTA+AGG 0.402159 7:+96199014 MsaT040058.1:CDS
TTGTTGTGGTTTGTTGTTGT+TGG 0.407241 7:-96198222 None:intergenic
TTGAAACTGAAAGGTAGAAA+TGG 0.413834 7:+96198875 MsaT040058.1:CDS
AATGGTTTGTTACAAAAGAA+AGG 0.427323 7:+96198443 MsaT040058.1:CDS
TAGTAACAAAGAAAGGGAAA+TGG 0.428604 7:+96199249 MsaT040058.1:CDS
ATGAAGACGAAGACGATGAA+TGG 0.431143 7:+96198918 MsaT040058.1:CDS
GTTAATGATATTCTTGGTAA+AGG 0.448596 7:+96198569 MsaT040058.1:CDS
AGAACAGGTAAACTATCATA+TGG 0.455868 7:+96199180 MsaT040058.1:CDS
ATCAGTTTCCAAAGGGATGA+TGG 0.456043 7:+96198475 MsaT040058.1:CDS
GAAGAGGAAGAAGAAGAATC+AGG 0.456394 7:+96198953 MsaT040058.1:CDS
TGTTACAAAAGAAAGGGAAA+TGG 0.458567 7:+96198450 MsaT040058.1:CDS
CACCATGGCATAGAATGAAA+TGG 0.467516 7:+96198327 MsaT040058.1:CDS
GTTGCAAATTGTTGTTTCCA+TGG 0.468486 7:-96198733 None:intergenic
TGCTGTTGTTGCTGTTGTTG+TGG 0.468978 7:-96198797 None:intergenic
ATAATGGCTGTTTATTACAT+TGG 0.475736 7:+96198374 MsaT040058.1:CDS
AAGATGGAGTGAAGAGTTGT+TGG 0.482165 7:+96199113 MsaT040058.1:CDS
TATCATAACAGTTGTGGCCA+TGG 0.484474 7:+96198716 MsaT040058.1:CDS
TGGAGGAGGAGAAGATGAAG+AGG 0.485154 7:+96198895 MsaT040058.1:CDS
AATTTAGTAGTAACAAAGAA+AGG 0.489528 7:+96199242 MsaT040058.1:CDS
ATGGTTTGTTACAAAAGAAA+GGG 0.489534 7:+96198444 MsaT040058.1:CDS
TGATTGATGAGGTTGATCAT+AGG 0.498163 7:-96198168 None:intergenic
GTCCATTTCATTCTATGCCA+TGG 0.498446 7:-96198329 None:intergenic
GGTGCAATTAGGAGAAGAAC+AGG 0.499399 7:+96199165 MsaT040058.1:CDS
AGTTCAATGATTTGAACAAA+AGG 0.501403 7:+96198537 MsaT040058.1:CDS
TGAAGACGAAGACGATGAAT+GGG 0.509736 7:+96198919 MsaT040058.1:CDS
AAAAGCAATGGATGAAGAAG+AGG 0.518548 7:+96199140 MsaT040058.1:CDS
GAAGAATCAGGAGAAGGTGG+TGG 0.520051 7:+96198965 MsaT040058.1:CDS
AGGTTGATGTTGATTTGTTG+TGG 0.522948 7:-96198236 None:intergenic
TGCATTCATCAAATGGGGTA+GGG 0.525561 7:+96198840 MsaT040058.1:CDS
GAAGAAGAATCAGGAGAAGG+TGG 0.525982 7:+96198962 MsaT040058.1:CDS
AGGTGGTGGTTATGGTTCAA+AGG 0.527876 7:+96198979 MsaT040058.1:CDS
TTAGGAATGTTGAAACTGAA+AGG 0.528480 7:+96198866 MsaT040058.1:CDS
ATTTAGTAGTAACAAAGAAA+GGG 0.534556 7:+96199243 MsaT040058.1:CDS
GGTGGTGGTTATGGTTCAAA+GGG 0.537283 7:+96198980 MsaT040058.1:CDS
TTGCATTCATCAAATGGGGT+AGG 0.539537 7:+96198839 MsaT040058.1:CDS
GTTTCATCTGCTCCAAAACA+TGG 0.550664 7:-96198275 None:intergenic
ATGGGAGGATGATTCTGAAG+AGG 0.551198 7:+96198937 MsaT040058.1:CDS
CTGAAAGGTAGAAATGGAGG+AGG 0.556127 7:+96198881 MsaT040058.1:CDS
TATTACATTGGTGATGAAGC+TGG 0.558817 7:+96198386 MsaT040058.1:CDS
AATGGAAATCAGTTTCCAAA+GGG 0.568552 7:+96198468 MsaT040058.1:CDS
TGTGGATGATGATGTTGCAG+TGG 0.570238 7:-96198779 None:intergenic
AACTGTTGCATCACTTGTGA+TGG 0.574352 7:-96199064 None:intergenic
AGATGGAGTGAAGAGTTGTT+GGG 0.577842 7:+96199114 MsaT040058.1:CDS
GAAGAAGAAGAATCAGGAGA+AGG 0.578759 7:+96198959 MsaT040058.1:CDS
CCTTGTTTGATTGATTGATG+AGG 0.589884 7:-96198179 None:intergenic
GTGATGAGTGTGTTGCAAGA+TGG 0.590136 7:+96199097 MsaT040058.1:CDS
AAAGCAATGGATGAAGAAGA+GGG 0.598249 7:+96199141 MsaT040058.1:CDS
CAGTGGTTGAGTCTCAGTTG+AGG 0.599148 7:-96198762 None:intergenic
AAACTGAAAGGTAGAAATGG+AGG 0.600866 7:+96198878 MsaT040058.1:CDS
CCTCATCAATCAATCAAACA+AGG 0.605701 7:+96198179 MsaT040058.1:CDS
AATGAAATGGACAGATACAA+TGG 0.606746 7:+96198340 MsaT040058.1:CDS
TTTGGAGCAGATGAAACCTC+TGG 0.607202 7:+96198281 MsaT040058.1:CDS
AAATGGAAATCAGTTTCCAA+AGG 0.612879 7:+96198467 MsaT040058.1:CDS
AACTTGTCTTCACATTGTTG+AGG 0.613435 7:-96198518 None:intergenic
ATGTTTGCATTCATCAAATG+GGG 0.639039 7:+96198835 MsaT040058.1:CDS
TGTGCTTATCATAACAGTTG+TGG 0.669194 7:+96198710 MsaT040058.1:CDS
AGACGAAGACGATGAATGGG+AGG 0.672078 7:+96198922 MsaT040058.1:CDS
GGAGCAGATGAAACCTCTGG+TGG 0.717638 7:+96198284 MsaT040058.1:CDS
ATGGTTCATCTTCATCACTG+AGG 0.725820 7:-96198256 None:intergenic
GCAATGGATGAAGAAGAGGG+TGG 0.776790 7:+96199144 MsaT040058.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 96198161 96199399 96198161 ID=MsaG040058
Chr7 mRNA 96198161 96199399 96198161 ID=MsaT040058.1;Parent=MsaG040058
Chr7 exon 96198161 96199399 96198161 ID=MsaT040058.1.exon1;Parent=MsaT040058.1
Chr7 CDS 96198161 96199399 96198161 ID=cds.MsaT040058.1;Parent=MsaT040058.1
Gene Sequence

>MsaT040058.1

ATGGTTTCCTATGATCAACCTCATCAATCAATCAAACAAGGCTACCCTTATTCATCCAAAACCAACAACAACAAACCACAACAAATCAACATCAACCTCAGTGATGAAGATGAACCATGTTTTGGAGCAGATGAAACCTCTGGTGGTGACCCAAAAAGAAAAGGATCACCATGGCATAGAATGAAATGGACAGATACAATGGTAAGACTCTTAATAATGGCTGTTTATTACATTGGTGATGAAGCTGGTACTTCAGAATTAAGTGCTGATAAAAAGAAAGCTAATGGTTTGTTACAAAAGAAAGGGAAATGGAAATCAGTTTCCAAAGGGATGATGGAAAAAGGGTACTATGTTTCACCTCAACAATGTGAAGACAAGTTCAATGATTTGAACAAAAGGTATAAAAGAGTTAATGATATTCTTGGTAAAGGAACAGCTTGTAGAGTTGTTGAAAATCAAACTTTGTTGGATACTATGGATTTGTCCGCAAAGTTGAAAGAAGAAGCTAGAAAACTTTTGAATTCTAAGCATCTTTTCTTGCGTGAAATGTGTGCTTATCATAACAGTTGTGGCCATGGAAACAACAATTTGCAACAACAGTCCTCAACTGAGACTCAACCACTGCAACATCATCATCCACAACAACAGCAACAACAGCAGCAGCAGCAGCAACAATGTTTGCATTCATCAAATGGGGTAGGGAGTTTAGGAATGTTGAAACTGAAAGGTAGAAATGGAGGAGGAGAAGATGAAGAGGATGAAGACGAAGACGATGAATGGGAGGATGATTCTGAAGAGGAAGAAGAAGAATCAGGAGAAGGTGGTGGTTATGGTTCAAAGGGTCATGAAGAAGATGAAAATATTGATCACTTAAGGAAAAAATCAAGAAAAGTTTCATCATTGCCATCACAAGTGATGCAACAGTTGAGTAGTGAAGTGATGAGTGTGTTGCAAGATGGAGTGAAGAGTTGTTGGGAGAAAAAGCAATGGATGAAGAAGAGGGTGGTGCAATTAGGAGAAGAACAGGTAAACTATCATATGGAAGCATTTGAGATTGAAAAACAAAGGTTGAAATGGGTGAAATTTAGTAGTAACAAAGAAAGGGAAATGGAGAGACAAAAACTTGAAAATGAAAGGAAGAGTTTGGAGATTGATAGAATGGTTTTGTTGCTACGTCAAAAAGAGCTTGAATTGCAAAACACTCAGCAGCTGCAACAGCAACAGCATTCTTCTACTTGA

Protein sequence

>MsaT040058.1

MVSYDQPHQSIKQGYPYSSKTNNNKPQQININLSDEDEPCFGADETSGGDPKRKGSPWHRMKWTDTMVRLLIMAVYYIGDEAGTSELSADKKKANGLLQKKGKWKSVSKGMMEKGYYVSPQQCEDKFNDLNKRYKRVNDILGKGTACRVVENQTLLDTMDLSAKLKEEARKLLNSKHLFLREMCAYHNSCGHGNNNLQQQSSTETQPLQHHHPQQQQQQQQQQQQCLHSSNGVGSLGMLKLKGRNGGGEDEEDEDEDDEWEDDSEEEEEESGEGGGYGSKGHEEDENIDHLRKKSRKVSSLPSQVMQQLSSEVMSVLQDGVKSCWEKKQWMKKRVVQLGEEQVNYHMEAFEIEKQRLKWVKFSSNKEREMERQKLENERKSLEIDRMVLLLRQKELELQNTQQLQQQQHSST*