AlfalfaGEDB | MsaG040076

M. sativa ssp. caerulea / MsaG040076

KEGG: K12836
GO: GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009987 GO:0010228 GO:0010467 GO:0016070 GO:0016071 GO:0022414 GO:0030628 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0036002 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0089701 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904
PFAM: PF00642.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG040076	XP_013450093.1	88.073	327	33	2	1	321	1	327	0.0	521

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG040076	sp\|Q9FMY5\|U2AFB_ARATH	57.655	307	90	5	1	292	1	282	1.68e-107	317

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG040076	tr\|A0A072U424\|A0A072U424_MEDTR	88.073	327	33	2	1	321	1	327	0.0	521

TFs/TRs:

Gene ID	Type	Classification
MsaG040076	TF	C3H

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001531	MsaG040076	0.824821	1.162320e-53	2.909591e-51
MsaG005189	MsaG040076	0.831137	3.568814e-55	1.067031e-52
MsaG005797	MsaG040076	0.806967	1.079176e-49	1.713429e-47
MsaG006529	MsaG040076	0.805243	2.479471e-49	3.780304e-47
MsaG007354	MsaG040076	0.804953	2.849710e-49	4.315594e-47
MsaG009389	MsaG040076	0.803780	4.988158e-49	7.351295e-47
MsaG018418	MsaG040076	0.808196	5.935094e-50	9.704378e-48
MsaG018786	MsaG040076	0.801639	1.372911e-48	1.926339e-46
MsaG026192	MsaG040076	0.806482	1.364730e-49	2.142312e-47
MsaG026830	MsaG040076	0.831834	2.407510e-55	7.346664e-53
MsaG026831	MsaG040076	0.812416	7.368027e-51	1.335013e-48
MsaG026985	MsaG040076	0.836592	1.564636e-56	5.497262e-54
MsaG027780	MsaG040076	0.804407	3.700150e-49	5.532995e-47
MsaG029461	MsaG040076	0.817334	6.055710e-52	1.242466e-49
MsaG032221	MsaG040076	0.829154	1.081587e-54	3.055147e-52
MsaG040076	MsaG044278	0.836399	1.751091e-56	6.117284e-54
MsaG040076	MsaG045371	0.861480	1.966499e-63	1.617573e-60
MsaG040076	MsaG045372	0.822880	3.297290e-53	7.830169e-51
MsaG040076	MsaG045373	0.818984	2.573956e-52	5.511680e-50
MsaG040076	MsaG046730	0.809652	2.907515e-50	4.923682e-48
MsaG040076	MsaG009144	0.833646	8.588925e-56	2.763293e-53
MsaG040076	MsaG019407	0.802699	8.331018e-49	1.197526e-46
MsaG040076	MsaG018678	0.810659	1.768131e-50	3.068311e-48
MsaG040076	MsaG019605	0.814494	2.587347e-51	4.938108e-49
MsaG040076	MsaG017935	0.803143	6.752064e-49	9.805317e-47
MsaG040076	MsaG033027	0.806659	1.252810e-49	1.974764e-47
MsaG040076	MsaG036984	0.815407	1.626404e-51	3.176564e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG040076	MtrunA17_Chr7g0265751	88.073	327	33	2	1	321	1	327	0.0	521
MsaG040076	MtrunA17_Chr2g0320801	73.000	200	40	2	1	200	1	186	7.15e-107	315
MsaG040076	MtrunA17_Chr1g0162821	72.105	190	39	2	1	190	1	176	8.59e-100	295
MsaG040076	MtrunA17_Chr2g0276901	36.735	98	59	1	92	186	28	125	1.55e-15	77.0
MsaG040076	MtrunA17_Chr2g0276891	55.102	49	22	0	9	57	184	232	2.27e-12	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG040076	AT5G42820.1	57.655	307	90	5	1	292	1	282	1.71e-108	317
MsaG040076	AT5G42820.2	57.655	307	90	5	1	292	1	282	1.71e-108	317
MsaG040076	AT1G27650.1	59.736	303	87	6	1	286	1	285	3.47e-105	310
MsaG040076	AT1G27650.2	52.964	253	84	6	51	286	1	235	2.22e-65	206
MsaG040076	AT1G10320.1	33.880	183	98	3	9	186	237	401	3.47e-32	127
MsaG040076	AT1G10320.2	33.880	183	98	3	9	186	228	392	3.71e-32	127
MsaG040076	AT3G44785.1	65.753	73	24	1	1	73	1	72	2.30e-29	107

Find 90 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTGTTCTTGGAGTTTATC+AGG	0.235787	7:-96363512	None:intergenic
ACTATGAGGCGTTCGTATTC+TGG	0.257507	7:+96365406	MsaT040076.1:CDS
CTTGAATATTTGTGACAATT+TGG	0.267256	7:+96363676	MsaT040076.1:CDS
GATTTGAGGAAGCGATTATT+TGG	0.276417	7:+96365318	MsaT040076.1:intron
TTATCAGGGTCAAGAGATTC+AGG	0.286148	7:-96363497	None:intergenic
TACATACATCGAAGTGTTCT+TGG	0.310686	7:-96363523	None:intergenic
TCGTATTCTGGCCGTGGTTC+TGG	0.312884	7:+96365418	MsaT040076.1:CDS
GAGAAGGAAGTTGGAGATTC+TGG	0.338699	7:+96365616	MsaT040076.1:CDS
TGAGGTTGTTGAGGTTGATT+AGG	0.344172	7:-96363455	None:intergenic
GAGCATTTAGCATCGATATT+CGG	0.354185	7:+96363266	MsaT040076.1:CDS
CTTTCTCTTTCTCCTCTGAT+AGG	0.355688	7:-96365553	None:intergenic
TGAGGTGGTTGAGGAGGTTG+AGG	0.362652	7:-96363473	None:intergenic
CTTTCTCCTCTGATAGGATT+CGG	0.367164	7:-96365547	None:intergenic
TGGTTCTGGTAGAAGGGATT+TGG	0.369893	7:+96365432	MsaT040076.1:CDS
CTGGCCGTGGTTCTGGTAGA+AGG	0.372740	7:+96365425	MsaT040076.1:CDS
GATTCAGGTTGAGGTGGTTG+AGG	0.376296	7:-96363482	None:intergenic
ATAAAGTTAAGGTTCATATC+AGG	0.384960	7:-96363422	None:intergenic
GAGTAGGATTGATAAAGTTA+AGG	0.396234	7:-96363433	None:intergenic
CTAGAATAGAACAATGGAAC+AGG	0.401571	7:+96365593	MsaT040076.1:CDS
TTTGAGGAGCGGAGCAAGTA+TGG	0.405295	7:+96363641	MsaT040076.1:CDS
TGTTGACTTCTCTCCTGTTA+CGG	0.406173	7:+96365110	MsaT040076.1:CDS
GCTTCTCGAAAATCCGTAAC+AGG	0.408045	7:-96365123	None:intergenic
TGACAATTTGGCTGATCATA+TGG	0.424836	7:+96363688	MsaT040076.1:CDS
TTCGGCTTGGCCTTCTACCA+TGG	0.427189	7:-96365471	None:intergenic
AGTAGGACTAATGGTGGGTT+TGG	0.429071	7:-96363372	None:intergenic
GCCTCATAGTTACGATTTCT+CGG	0.435284	7:-96365393	None:intergenic
CTCATGGCTCCCATGGTAGA+AGG	0.441236	7:+96365461	MsaT040076.1:CDS
TGTTGAGGTTGATTAGGAGT+AGG	0.451883	7:-96363449	None:intergenic
GATTATTTGGAAGAAGTAGT+AGG	0.454782	7:+96365331	MsaT040076.1:CDS
TGCATCTAAAGAAGATTAGC+AGG	0.461797	7:+96365202	MsaT040076.1:CDS
CCTCATAGTTACGATTTCTC+GGG	0.473936	7:-96365392	None:intergenic
TCAGGTTGAGGTGGTTGAGG+AGG	0.483616	7:-96363479	None:intergenic
AAAGAGAAAGTAGTGTAGAA+AGG	0.483749	7:+96365566	MsaT040076.1:CDS
TGGAACAGGGAGAAGGAAGT+TGG	0.485919	7:+96365607	MsaT040076.1:CDS
CTATGCCGAGGACTTCGGCT+TGG	0.492802	7:-96365484	None:intergenic
GGAGAGAAGTCAACAATGAT+TGG	0.494146	7:-96365102	None:intergenic
GGACTCCTGTCTCTTCCCAT+TGG	0.497688	7:-96365526	None:intergenic
GCTCCGATCTTGAAGTAGAA+TGG	0.500982	7:-96363314	None:intergenic
CTGGTAGAAGGGATTTGGAC+AGG	0.507028	7:+96365437	MsaT040076.1:CDS
TTTATATGAAGATCAATTTG+AGG	0.508320	7:+96363625	MsaT040076.1:CDS
TCGGCTTGGCCTTCTACCAT+GGG	0.509181	7:-96365470	None:intergenic
TGAGGAGGTTGAGGTTGTTG+AGG	0.510776	7:-96363464	None:intergenic
CTCATAGTTACGATTTCTCG+GGG	0.513609	7:-96365391	None:intergenic
GGGTCAAGAGATTCAGGTTG+AGG	0.514083	7:-96363491	None:intergenic
TTGGAAGAAGTAGTAGGCGT+TGG	0.517691	7:+96365337	MsaT040076.1:CDS
GACAGGTCTCATGGCTCCCA+TGG	0.524520	7:+96365454	MsaT040076.1:CDS
AGGCGTTCGTATTCTGGCCG+TGG	0.526621	7:+96365412	MsaT040076.1:CDS
GAGAACAAGAGTAGGACTAA+TGG	0.530039	7:-96363381	None:intergenic
AGGCGGAGTAGAAGTCCAAT+GGG	0.531064	7:+96365511	MsaT040076.1:CDS
ATCAGGACGTTGATACATGT+TGG	0.531267	7:-96363405	None:intergenic
GAAGTCCAATGGGAAGAGAC+AGG	0.531891	7:+96365521	MsaT040076.1:CDS
TGTATATGTTCAGTATAAAG+AGG	0.534599	7:+96363812	MsaT040076.1:CDS
GAAGGCCAAGCCGAAGTCCT+CGG	0.540971	7:+96365479	MsaT040076.1:CDS
ACCGTAGTGGAAGCAGAAGC+CGG	0.541010	7:+96365364	MsaT040076.1:CDS
TCCGGCTTCTGCTTCCACTA+CGG	0.545385	7:-96365365	None:intergenic
TGGCCGTGGTTCTGGTAGAA+GGG	0.546507	7:+96365426	MsaT040076.1:CDS
TTCCTCTATGCCGAGGACTT+CGG	0.552971	7:-96365489	None:intergenic
AGGGATTTGGACAGGTCTCA+TGG	0.560557	7:+96365445	MsaT040076.1:CDS
AGTGTTCTTGGAGTTTATCA+GGG	0.562952	7:-96363511	None:intergenic
AACAATGATTATGAAGAGGC+GGG	0.563713	7:+96365658	MsaT040076.1:CDS
AATGCTCTTATGAATCTCAC+TGG	0.570244	7:+96363849	MsaT040076.1:CDS
TGTCCATTCTACTTCAAGAT+CGG	0.570630	7:+96363311	MsaT040076.1:CDS
CCCGAGAAATCGTAACTATG+AGG	0.573093	7:+96365392	MsaT040076.1:CDS
AAGGCGGAGTAGAAGTCCAA+TGG	0.574467	7:+96365510	MsaT040076.1:CDS
GAAGTCCTCGGCATAGAGGA+AGG	0.592810	7:+96365491	MsaT040076.1:CDS
AGAACAATGGAACAGGGAGA+AGG	0.596602	7:+96365600	MsaT040076.1:CDS
TCATAAGAGCATTAGCAGCA+TGG	0.602037	7:-96363839	None:intergenic
AGCCGAAGTCCTCGGCATAG+AGG	0.604215	7:+96365487	MsaT040076.1:CDS
TAACAATGATTATGAAGAGG+CGG	0.605105	7:+96365657	MsaT040076.1:CDS
AGAAGTCAACAATGATTGGT+CGG	0.606403	7:-96365098	None:intergenic
CATAGTTACGATTTCTCGGG+GGG	0.607441	7:-96365389	None:intergenic
ATGAAGATCAATTTGAGGAG+CGG	0.608897	7:+96363630	MsaT040076.1:CDS
AACAAGAGTAGGACTAATGG+TGG	0.614682	7:-96363378	None:intergenic
TCAAGAGATTCAGGTTGAGG+TGG	0.615585	7:-96363488	None:intergenic
AAGATCGGAGCTTGTAGACA+TGG	0.618937	7:+96363326	MsaT040076.1:CDS
CTCCGCCTTCCTCTATGCCG+AGG	0.622022	7:-96365496	None:intergenic
GAGTAACAATGATTATGAAG+AGG	0.626539	7:+96365654	MsaT040076.1:CDS
AGTTGGAGATTCTGGTAACA+AGG	0.630652	7:+96365624	MsaT040076.1:CDS
AGGAGTCCGAATCCTATCAG+AGG	0.630679	7:+96365541	MsaT040076.1:CDS
GAAGAGAATGTATGCAATCG+AGG	0.636366	7:+96365162	MsaT040076.1:CDS
ATGTTGGAGAGAACAAGAGT+AGG	0.650220	7:-96363389	None:intergenic
GGAGAGCTAGAATAGAACAA+TGG	0.656515	7:+96365587	MsaT040076.1:CDS
TCATAGTTACGATTTCTCGG+GGG	0.667828	7:-96365390	None:intergenic
AGGCGTTGGAATGACCGTAG+TGG	0.684717	7:+96365351	MsaT040076.1:CDS
TAGAATAGAACAATGGAACA+GGG	0.687714	7:+96365594	MsaT040076.1:CDS
AATCCCTTCTACCAGAACCA+CGG	0.694334	7:-96365429	None:intergenic
ACAAGAGTAGGACTAATGGT+GGG	0.704223	7:-96363377	None:intergenic
GAGAATGTATGCAATCGAGG+TGG	0.723621	7:+96365165	MsaT040076.1:CDS
GTCCTCGGCATAGAGGAAGG+CGG	0.728288	7:+96365494	MsaT040076.1:CDS
TGGTATGAAGACGAGAACAG+CGG	0.740225	7:-96363352	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	96363260	96365720	96363260	ID=MsaG040076
Chr7	mRNA	96363260	96365720	96363260	ID=MsaT040076.1;Parent=MsaG040076
Chr7	exon	96363260	96363538	96363260	ID=MsaT040076.1.exon1;Parent=MsaT040076.1
Chr7	CDS	96363260	96363538	96363260	ID=cds.MsaT040076.1;Parent=MsaT040076.1
Chr7	exon	96363623	96363709	96363623	ID=MsaT040076.1.exon2;Parent=MsaT040076.1
Chr7	CDS	96363623	96363709	96363623	ID=cds.MsaT040076.1;Parent=MsaT040076.1
Chr7	exon	96363804	96363885	96363804	ID=MsaT040076.1.exon3;Parent=MsaT040076.1
Chr7	CDS	96363804	96363885	96363804	ID=cds.MsaT040076.1;Parent=MsaT040076.1
Chr7	exon	96365097	96365223	96365097	ID=MsaT040076.1.exon4;Parent=MsaT040076.1
Chr7	CDS	96365097	96365223	96365097	ID=cds.MsaT040076.1;Parent=MsaT040076.1
Chr7	exon	96365330	96365720	96365330	ID=MsaT040076.1.exon5;Parent=MsaT040076.1
Chr7	CDS	96365330	96365720	96365330	ID=cds.MsaT040076.1;Parent=MsaT040076.1

Gene Sequence

>MsaT040076.1

ATGGCGGAGCATTTAGCATCGATATTCGGAACAGAGAAAGACAGAGTTAACTGTCCATTCTACTTCAAGATCGGAGCTTGTAGACATGGTGACCGCTGTTCTCGTCTTCATACCAAACCCACCATTAGTCCTACTCTTGTTCTCTCCAACATGTATCAACGTCCTGATATGAACCTTAACTTTATCAATCCTACTCCTAATCAACCTCAACAACCTCAACCTCCTCAACCACCTCAACCTGAATCTCTTGACCCTGATAAACTCCAAGAACACTTCGATGATTTATATGAAGATCAATTTGAGGAGCGGAGCAAGTATGGTCAAATTCAGAGCTTGAATATTTGTGACAATTTGGCTGATCATATGGTTGGCAATGTATATGTTCAGTATAAAGAGGAAGACCATGCTGCTAATGCTCTTATGAATCTCACTGGAAGATTTTATTCAGGCCGACCAATCATTGTTGACTTCTCTCCTGTTACGGATTTTCGAGAAGCTACTTGTAGGCAGTACGAAGAGAATGTATGCAATCGAGGTGGCTACTGCAACTTTATGCATCTAAAGAAGATTAGCAGCGATTATTTGGAAGAAGTAGTAGGCGTTGGAATGACCGTAGTGGAAGCAGAAGCCGGAGCCCCCCGAGAAATCGTAACTATGAGGCGTTCGTATTCTGGCCGTGGTTCTGGTAGAAGGGATTTGGACAGGTCTCATGGCTCCCATGGTAGAAGGCCAAGCCGAAGTCCTCGGCATAGAGGAAGGCGGAGTAGAAGTCCAATGGGAAGAGACAGGAGTCCGAATCCTATCAGAGGAGAAAGAGAAAGTAGTGTAGAAAGGAGAGCTAGAATAGAACAATGGAACAGGGAGAAGGAAGTTGGAGATTCTGGTAACAAGGACAATAAGAGTAACAATGATTATGAAGAGGCGGGCCTAAAAAATGGCAGCGAGTCTGGCAATCATCAGACATAG

Protein sequence

>MsaT040076.1

MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPTISPTLVLSNMYQRPDMNLNFINPTPNQPQQPQPPQPPQPESLDPDKLQEHFDDLYEDQFEERSKYGQIQSLNICDNLADHMVGNVYVQYKEEDHAANALMNLTGRFYSGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISSDYLEEVVGVGMTVVEAEAGAPREIVTMRRSYSGRGSGRRDLDRSHGSHGRRPSRSPRHRGRRSRSPMGRDRSPNPIRGERESSVERRARIEQWNREKEVGDSGNKDNKSNNDYEEAGLKNGSESGNHQT*