AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040120 MtrunA17_Chr7g0266211 97.917 432 7 2 1 431 1 431 0.0 860
MsaG040120 MtrunA17_Chr8g0355121 35.112 356 134 11 37 388 23 285 4.72e-46 161
MsaG040120 MtrunA17_Chr4g0044771 34.709 412 151 17 43 406 22 363 6.04e-46 162
MsaG040120 MtrunA17_Chr3g0080341 35.854 357 152 11 38 394 20 299 1.38e-44 157
MsaG040120 MtrunA17_Chr2g0321381 50.350 143 57 6 43 185 26 154 2.07e-36 135
MsaG040120 MtrunA17_Chr2g0321381 46.154 143 48 4 266 393 161 289 7.33e-19 86.7
MsaG040120 MtrunA17_Chr2g0321411 48.551 138 59 5 44 181 13 138 4.77e-32 123
MsaG040120 MtrunA17_Chr2g0321411 52.381 126 47 3 298 419 165 281 3.34e-30 118
MsaG040120 MtrunA17_Chr3g0133971 48.148 135 48 4 44 178 32 144 3.47e-28 115
MsaG040120 MtrunA17_Chr3g0133971 46.043 139 67 3 280 412 283 419 3.55e-22 98.2
MsaG040120 MtrunA17_Chr6g0460301 38.255 149 69 3 41 182 18 150 2.60e-21 94.0
MsaG040120 MtrunA17_Chr7g0254991 35.766 137 66 4 44 178 21 137 1.26e-17 83.6
MsaG040120 MtrunA17_Chr7g0254991 67.164 67 20 2 330 395 233 298 1.49e-17 83.2
MsaG040120 MtrunA17_Chr4g0051101 37.097 124 65 3 286 402 71 188 1.80e-15 74.7
MsaG040120 MtrunA17_Chr1g0201751 53.030 66 31 0 340 405 157 222 8.21e-13 68.2
MsaG040120 MtrunA17_Chr5g0440841 36.090 133 67 2 44 176 27 141 1.80e-12 67.4
MsaG040120 MtrunA17_Chr5g0440841 58.621 58 24 0 331 388 184 241 3.83e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040120 AT2G36270.1 47.083 480 167 12 1 431 1 442 1.28e-114 343
MsaG040120 AT2G36270.2 47.083 480 167 12 1 431 1 442 1.28e-114 343
MsaG040120 AT2G36270.3 47.556 450 149 12 1 401 1 412 7.99e-107 323
MsaG040120 AT1G45249.7 36.855 407 181 14 44 407 41 414 4.37e-50 176
MsaG040120 AT1G45249.6 36.855 407 181 14 44 407 41 414 4.37e-50 176
MsaG040120 AT1G45249.1 36.364 407 183 14 44 407 26 399 4.30e-48 170
MsaG040120 AT1G45249.3 35.613 424 185 16 38 407 21 410 7.00e-45 161
MsaG040120 AT1G49720.1 31.633 392 224 9 29 403 6 370 5.74e-35 134
MsaG040120 AT1G49720.3 31.633 392 224 9 29 403 6 370 5.74e-35 134
MsaG040120 AT3G44460.1 51.408 142 53 4 38 178 35 161 1.52e-31 123
MsaG040120 AT3G44460.1 53.211 109 48 1 321 429 224 329 6.67e-21 93.6
MsaG040120 AT3G56850.1 47.368 152 60 5 27 178 5 136 5.86e-28 112
MsaG040120 AT3G56850.1 67.797 59 18 1 331 388 211 269 1.28e-15 77.4
MsaG040120 AT1G49720.2 41.212 165 71 5 29 181 6 156 4.16e-27 112
MsaG040120 AT1G49720.2 55.556 72 32 0 331 402 298 369 2.17e-16 81.3
MsaG040120 AT5G42910.1 27.208 419 227 13 26 421 7 370 4.96e-27 111
MsaG040120 AT3G19290.4 44.138 145 72 4 38 178 34 173 3.99e-25 107
MsaG040120 AT3G19290.4 45.614 114 49 3 289 402 309 409 9.44e-18 85.1
MsaG040120 AT3G19290.1 44.138 145 72 4 38 178 34 173 6.50e-25 106
MsaG040120 AT3G19290.1 46.667 120 50 4 289 408 309 414 4.85e-18 86.3
MsaG040120 AT3G19290.5 44.138 145 72 4 38 178 34 173 6.50e-25 106
MsaG040120 AT3G19290.5 46.667 120 50 4 289 408 309 414 4.85e-18 86.3
MsaG040120 AT3G19290.3 44.138 145 72 4 38 178 34 173 6.62e-25 106
MsaG040120 AT3G19290.3 46.667 120 51 3 289 408 309 415 3.40e-19 89.7
MsaG040120 AT3G19290.2 44.138 145 72 4 38 178 34 173 8.67e-25 105
MsaG040120 AT4G34000.3 40.606 165 72 5 38 179 27 188 1.42e-24 105
MsaG040120 AT4G34000.3 53.659 82 31 1 329 403 357 438 3.39e-17 84.0
MsaG040120 AT4G34000.1 40.476 168 74 5 35 179 24 188 1.44e-24 105
MsaG040120 AT4G34000.1 57.333 75 32 0 329 403 357 431 4.26e-19 89.7
MsaG040120 AT4G34000.4 40.476 168 74 5 35 179 24 188 1.44e-24 105
MsaG040120 AT4G34000.4 57.333 75 32 0 329 403 357 431 4.26e-19 89.7
MsaG040120 AT4G34000.2 40.476 168 74 5 35 179 24 188 1.44e-24 105
MsaG040120 AT4G34000.2 57.333 75 32 0 329 403 357 431 4.26e-19 89.7
MsaG040120 AT2G41070.5 34.872 195 77 7 25 210 3 156 7.18e-21 91.7
MsaG040120 AT2G41070.5 67.241 58 19 0 331 388 177 234 6.80e-16 77.4
MsaG040120 AT2G41070.3 34.872 195 77 7 25 210 3 156 1.57e-20 91.3
MsaG040120 AT2G41070.3 64.615 65 22 1 331 395 177 240 2.81e-16 79.0
MsaG040120 AT2G41070.1 34.872 195 77 7 25 210 3 156 1.57e-20 91.3
MsaG040120 AT2G41070.1 64.615 65 22 1 331 395 177 240 2.81e-16 79.0
MsaG040120 AT2G41070.2 34.872 195 77 7 25 210 3 156 1.57e-20 91.3
MsaG040120 AT2G41070.2 64.615 65 22 1 331 395 177 240 2.81e-16 79.0
MsaG040120 AT2G41070.4 34.872 195 77 7 25 210 12 165 1.96e-20 90.9
MsaG040120 AT2G41070.4 64.615 65 22 1 331 395 186 249 2.67e-16 79.0
MsaG040120 AT5G44080.1 44.762 105 55 2 323 424 207 311 5.12e-15 75.9
MsaG040120 AT1G45249.10 52.222 90 31 4 290 371 278 363 1.42e-14 75.5
MsaG040120 AT1G45249.5 52.222 90 31 4 290 371 278 363 1.42e-14 75.5
MsaG040120 AT2G17770.2 41.026 117 51 3 271 387 69 167 4.09e-14 71.2
MsaG040120 AT1G45249.4 47.573 103 36 5 290 378 278 376 1.33e-13 72.8
MsaG040120 AT1G45249.8 47.573 103 36 5 290 378 278 376 1.33e-13 72.8
MsaG040120 AT1G45249.2 47.573 103 36 5 290 378 278 376 1.33e-13 72.8
MsaG040120 AT1G45249.9 47.573 103 36 5 290 378 278 376 1.33e-13 72.8
MsaG040120 AT1G03970.1 50.000 76 36 1 328 403 173 246 2.45e-12 67.4
MsaG040120 AT4G35900.1 33.951 162 89 6 240 394 114 264 5.92e-12 66.6

Find 152 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAACTCGGGTGGTCAATT+TGG 0.166727 7:+96946010 MsaT040120.1:CDS
ATGAGTTTCTAAGTAGTATT+TGG 0.209852 7:+96945273 MsaT040120.1:CDS
CAGGAATCGATAACGAATTT+TGG 0.216092 7:-96945403 None:intergenic
GTTGGAGAGCCTTCGGGTTC+GGG 0.231478 7:+96945806 MsaT040120.1:CDS
CAGGCTGCTTCATTTGCTAT+TGG 0.248337 7:+96945716 MsaT040120.1:CDS
GTGACAGCGGCAAGAATTTC+GGG 0.248709 7:+96945237 MsaT040120.1:CDS
AGTTGGAGAGCCTTCGGGTT+CGG 0.262820 7:+96945805 MsaT040120.1:CDS
GATGACATTGGAGGATTTCT+TGG 0.268533 7:+96945553 MsaT040120.1:CDS
TGTGACAGCGGCAAGAATTT+CGG 0.276728 7:+96945236 MsaT040120.1:CDS
TGGTTAAAGCAGGGGTAGTT+AGG 0.281516 7:+96945573 MsaT040120.1:CDS
AATGGAGGGGCGGGCGGTTA+TGG 0.286276 7:+96945917 MsaT040120.1:CDS
GCCATTGTAGAGTTGTTATT+AGG 0.296465 7:-96945692 None:intergenic
CCGCCGCCAGCGGTTTGTTA+TGG 0.308148 7:+96945881 MsaT040120.1:CDS
GTTGTTGCTGTTATTATTGT+TGG 0.310662 7:-96945327 None:intergenic
TGTGGCAGTGGCACAAACTA+TGG 0.315000 7:+96945940 MsaT040120.1:CDS
ACCCCTCGACAACCGACTTT+TGG 0.322363 7:+96945527 MsaT040120.1:CDS
ACCTGTTAGTCCGGTTTCTT+CGG 0.327709 7:+96945973 MsaT040120.1:CDS
AAAACTCGGGTGGTCAATTT+GGG 0.341515 7:+96946011 MsaT040120.1:CDS
ACACTGCGGCGTAATGCTGC+TGG 0.344613 7:-96945667 None:intergenic
TTACCTCATGCAGTGTTTAG+AGG 0.347444 7:+96946706 MsaT040120.1:intron
GGAAAACTGTTGAACAAGTT+TGG 0.348644 7:+96945438 MsaT040120.1:CDS
TTGCTATTGGCGGCGGCGGC+GGG 0.353891 7:+96945729 MsaT040120.1:CDS
CTCTCCACCACCCTTTCAAC+AGG 0.360473 7:-96946079 None:intergenic
CACTGCGGCGTAATGCTGCT+GGG 0.364765 7:-96945666 None:intergenic
TGGAGGAGTTGGAGAGCCTT+CGG 0.365531 7:+96945799 MsaT040120.1:CDS
TCCGAAGAAACCGGACTAAC+AGG 0.372237 7:-96945974 None:intergenic
GTCGAGGGTGAGTGAATATA+TGG 0.374227 7:-96945195 None:intergenic
TTTGCTATTGGCGGCGGCGG+CGG 0.374673 7:+96945728 MsaT040120.1:CDS
CTTCGGGTTCGGGGTATGTT+GGG 0.383128 7:+96945816 MsaT040120.1:CDS
CAAACCGCTGGCGGCGGCGG+AGG 0.394088 7:-96945875 None:intergenic
ACTGCTGCTGCGGCAGGTGG+TGG 0.397437 7:-96945629 None:intergenic
TAGAAACTCATCCATGTTCA+TGG 0.401819 7:-96945261 None:intergenic
AGAGAATTCTCAACTGAAAC+AGG 0.410221 7:+96946454 MsaT040120.1:CDS
TAAGAGAAGGTGAGATGAAT+TGG 0.412357 7:+96945072 MsaT040120.1:CDS
CCGGTTTCTTCGGATGGGAT+TGG 0.415620 7:+96945983 MsaT040120.1:CDS
GGAGGAGTTGGAGAGCCTTC+GGG 0.416356 7:+96945800 MsaT040120.1:CDS
CCTTCGGGTTCGGGGTATGT+TGG 0.416821 7:+96945815 MsaT040120.1:CDS
CACCTCCCTGTGCCACCGTT+TGG 0.419295 7:-96945778 None:intergenic
AAGATCTAGAGCCAGAAAAC+AGG 0.420885 7:+96946141 MsaT040120.1:CDS
TGTTGGATGCAGCTTGTTGT+TGG 0.421539 7:-96945310 None:intergenic
CGGCGTAATGCTGCTGGGGC+CGG 0.426351 7:-96945661 None:intergenic
GCACAGGGAGGTGGAGGAGT+TGG 0.427569 7:+96945788 MsaT040120.1:CDS
GTTAGTCCGGTTTCTTCGGA+TGG 0.429323 7:+96945977 MsaT040120.1:CDS
TCATTTGCTATTGGCGGCGG+CGG 0.434400 7:+96945725 MsaT040120.1:CDS
CCCTGTGCCACCGTTTGGTA+TGG 0.438711 7:-96945773 None:intergenic
GGTGGTGGTGCTATGGCAAC+CGG 0.443970 7:-96945614 None:intergenic
GAGGGAGAGTGGTGAATGGA+GGG 0.444211 7:+96945903 MsaT040120.1:CDS
GATGGGCGGACCTGTTAGTC+CGG 0.445248 7:+96945964 MsaT040120.1:CDS
TCAGAAAGGGATTAGCAAAC+AGG 0.447868 7:+96945367 MsaT040120.1:CDS
ATTGATATGAATGGTTTAAG+AGG 0.448244 7:+96946034 MsaT040120.1:CDS
GGAAGAAAGAGGATGGTTGA+TGG 0.453215 7:+96946055 MsaT040120.1:CDS
GAGGATTTCTTGGTTAAAGC+AGG 0.456229 7:+96945563 MsaT040120.1:CDS
GATGACACTGCTGCTGCGGC+AGG 0.456822 7:-96945635 None:intergenic
TGGTGGAGAGAAGGCAGAGG+AGG 0.457363 7:+96946092 MsaT040120.1:CDS
GACTAGGGATAGTATAGGTA+CGG 0.462211 7:+96945847 MsaT040120.1:CDS
AATGTCATTTCTTCATCGCT+TGG 0.465109 7:+96945158 MsaT040120.1:CDS
GCCATACCAAACGGTGGCAC+AGG 0.467096 7:+96945772 MsaT040120.1:CDS
ACGGTGGCACAGGGAGGTGG+AGG 0.470179 7:+96945782 MsaT040120.1:CDS
GGGCGGTTATGGTGTGGCAG+TGG 0.471720 7:+96945928 MsaT040120.1:CDS
GGGAAGGAGAAGAAGCGATC+AGG 0.473369 7:+96945123 MsaT040120.1:CDS
AGGGGCGGGCGGTTATGGTG+TGG 0.475144 7:+96945922 MsaT040120.1:CDS
TAACAAACCGCTGGCGGCGG+CGG 0.477710 7:-96945878 None:intergenic
CCGCCAGCGGTTTGTTATGG+AGG 0.477954 7:+96945884 MsaT040120.1:CDS
CAAACGGTGGCACAGGGAGG+TGG 0.478151 7:+96945779 MsaT040120.1:CDS
CGATTCCTGCACCTCTCTGC+AGG 0.479043 7:+96945417 MsaT040120.1:CDS
GGTTGATGGTCCTGTTGAAA+GGG 0.481262 7:+96946069 MsaT040120.1:CDS
CCAATCCCATCCGAAGAAAC+CGG 0.481309 7:-96945983 None:intergenic
GGTTAAAGCAGGGGTAGTTA+GGG 0.481908 7:+96945574 MsaT040120.1:CDS
AGAGAGGAGAAGAAGACAAC+AGG 0.484243 7:+96946587 MsaT040120.1:CDS
TTAGTCCGGTTTCTTCGGAT+GGG 0.486374 7:+96945978 MsaT040120.1:CDS
GACACTGCTGCTGCGGCAGG+TGG 0.491945 7:-96945632 None:intergenic
AGGGAGAGTGGTGAATGGAG+GGG 0.491999 7:+96945904 MsaT040120.1:CDS
GCTGCTTCATTTGCTATTGG+CGG 0.495000 7:+96945719 MsaT040120.1:CDS
GAGAGTGGTGAATGGAGGGG+CGG 0.499932 7:+96945907 MsaT040120.1:CDS
AGTGGCACAAACTATGGGGA+TGG 0.502987 7:+96945946 MsaT040120.1:CDS
CAATTTGGGATTGATATGAA+TGG 0.505237 7:+96946025 MsaT040120.1:CDS
AGAGTGGTGAATGGAGGGGC+GGG 0.505464 7:+96945908 MsaT040120.1:CDS
TGCGGCAGGTGGTGGTGCTA+TGG 0.506524 7:-96945621 None:intergenic
GGGTGGTGGAGAGAAGGCAG+AGG 0.506953 7:+96946089 MsaT040120.1:CDS
GGAGGGAGAGTGGTGAATGG+AGG 0.509570 7:+96945902 MsaT040120.1:CDS
CCATAACAAACCGCTGGCGG+CGG 0.510979 7:-96945881 None:intergenic
ACTTGTCTGCAGCTCAGAAA+GGG 0.519532 7:+96945354 MsaT040120.1:CDS
CAACATTGTTGATGTTGTGA+TGG 0.520957 7:-96945487 None:intergenic
GTGGCAGTGGCACAAACTAT+GGG 0.520968 7:+96945941 MsaT040120.1:CDS
AACTTGTCTGCAGCTCAGAA+AGG 0.521964 7:+96945353 MsaT040120.1:CDS
CGCCAAAAGTCGGTTGTCGA+GGG 0.522444 7:-96945529 None:intergenic
AAGAATATGGTGGTAAGAGA+AGG 0.522714 7:+96945059 None:intergenic
GCTTCATTTGCTATTGGCGG+CGG 0.528163 7:+96945722 MsaT040120.1:CDS
TATGGAGGGAGAGTGGTGAA+TGG 0.528806 7:+96945899 MsaT040120.1:CDS
CGCCAGCGGTTTGTTATGGA+GGG 0.528969 7:+96945885 MsaT040120.1:CDS
ACTAGGGATAGTATAGGTAC+GGG 0.531284 7:+96945848 MsaT040120.1:CDS
AGAAACTGAGAGGCTTAAGA+AGG 0.532363 7:+96946771 MsaT040120.1:CDS
TAACAACTCTACAATGGCTC+AGG 0.534100 7:+96945697 MsaT040120.1:CDS
TGTTGGGAATGGTAAGACTA+GGG 0.535160 7:+96945832 MsaT040120.1:CDS
GAATTTCGGGTCCATGAACA+TGG 0.544850 7:+96945250 MsaT040120.1:CDS
TTTAAGAGGAAGAAAGAGGA+TGG 0.548041 7:+96946048 MsaT040120.1:CDS
CGCTGTCACAGAGGCTGTGT+TGG 0.549331 7:-96945223 None:intergenic
TCGCCAAAAGTCGGTTGTCG+AGG 0.549884 7:-96945530 None:intergenic
TTGAAAGGGTGGTGGAGAGA+AGG 0.555209 7:+96946083 MsaT040120.1:CDS
GTGGCACAAACTATGGGGAT+GGG 0.557884 7:+96945947 MsaT040120.1:CDS
GGAACAACAGTCTGGTATGC+CGG 0.558038 7:+96945595 MsaT040120.1:CDS
CTCCCTCCATAACAAACCGC+TGG 0.558325 7:-96945887 None:intergenic
GTAGTTAGGGAACAACAGTC+TGG 0.560019 7:+96945587 MsaT040120.1:CDS
TTCTCAACTGAAACAGGCAC+TGG 0.564684 7:+96946460 MsaT040120.1:CDS
TGGTTGATGGTCCTGTTGAA+AGG 0.565970 7:+96946068 MsaT040120.1:CDS
GGCTGTGTTGGAACTCGTCG+AGG 0.566650 7:-96945211 None:intergenic
GGTTCGGGGTATGTTGGGAA+TGG 0.567692 7:+96945821 MsaT040120.1:CDS
CAGCAGCAGTGTCATCTCAC+CGG 0.569711 7:+96945642 MsaT040120.1:CDS
ATGGTTTAAGAGGAAGAAAG+AGG 0.570839 7:+96946044 MsaT040120.1:CDS
TTGGAGAGCCTTCGGGTTCG+GGG 0.572733 7:+96945807 MsaT040120.1:CDS
TGTGCCACCGTTTGGTATGG+CGG 0.573089 7:-96945770 None:intergenic
AGGATTTCTTGGTTAAAGCA+GGG 0.574066 7:+96945564 MsaT040120.1:CDS
TGATGGTCCTGTTGAAAGGG+TGG 0.574869 7:+96946072 MsaT040120.1:CDS
TTCTTCAGGCTGAACTAGAG+AGG 0.575021 7:+96946571 MsaT040120.1:intron
GGTAAGACTAGGGATAGTAT+AGG 0.577774 7:+96945842 MsaT040120.1:CDS
ACTGCGGCGTAATGCTGCTG+GGG 0.580500 7:-96945665 None:intergenic
GTGAGATGAATTGGCAACAA+GGG 0.583790 7:+96945081 MsaT040120.1:CDS
CCATACCAAACGGTGGCACA+GGG 0.583912 7:+96945773 MsaT040120.1:CDS
TGGGATTGGAAACGAAAACT+CGG 0.584083 7:+96945997 MsaT040120.1:CDS
GTAGAATCATTGCAGAGGGA+AGG 0.584321 7:+96945107 MsaT040120.1:CDS
ATGTTGGGAATGGTAAGACT+AGG 0.593588 7:+96945831 MsaT040120.1:CDS
GGTTGCGCCGCCATACCAAA+CGG 0.595175 7:+96945763 MsaT040120.1:CDS
GTATCCTCCGCCGCCGCCAG+CGG 0.598153 7:+96945871 MsaT040120.1:CDS
GTGGTGAATGGAGGGGCGGG+CGG 0.598279 7:+96945911 MsaT040120.1:CDS
CAACACAGCCTCTGTGACAG+CGG 0.601697 7:+96945224 MsaT040120.1:CDS
CGGCGGCGGGAATCTGAATG+TGG 0.602274 7:+96945742 MsaT040120.1:CDS
AATGTCATCTCGCCAAAAGT+CGG 0.605885 7:-96945539 None:intergenic
GTGAGATGACACTGCTGCTG+CGG 0.608010 7:-96945639 None:intergenic
TGGTCCTGTTGAAAGGGTGG+TGG 0.612440 7:+96946075 MsaT040120.1:CDS
GGAGGTAGAATCATTGCAGA+GGG 0.612581 7:+96945103 MsaT040120.1:CDS
GGTGAGATGAATTGGCAACA+AGG 0.616844 7:+96945080 MsaT040120.1:CDS
CGGTTGTCGAGGGGTAGACT+CGG 0.617759 7:-96945519 None:intergenic
TCCTAATAACAACTCTACAA+TGG 0.627967 7:+96945691 MsaT040120.1:CDS
GCACAAACTATGGGGATGGG+CGG 0.628434 7:+96945950 MsaT040120.1:CDS
GGTTTGTTATGGAGGGAGAG+TGG 0.634679 7:+96945892 MsaT040120.1:CDS
GATGAATTGGCAACAAGGGG+AGG 0.635893 7:+96945085 MsaT040120.1:CDS
TGAGATGAATTGGCAACAAG+GGG 0.639163 7:+96945082 MsaT040120.1:CDS
CCTCCATAACAAACCGCTGG+CGG 0.640926 7:-96945884 None:intergenic
GGATTTCTTGGTTAAAGCAG+GGG 0.646725 7:+96945565 MsaT040120.1:CDS
CCAACATACCCCGAACCCGA+AGG 0.649040 7:-96945815 None:intergenic
GCCAAAAGTCGGTTGTCGAG+GGG 0.657167 7:-96945528 None:intergenic
GCTGTGTTGGAACTCGTCGA+GGG 0.657810 7:-96945210 None:intergenic
GGGATTGGAAACGAAAACTC+GGG 0.662976 7:+96945998 MsaT040120.1:CDS
GGGAGGTAGAATCATTGCAG+AGG 0.672753 7:+96945102 MsaT040120.1:CDS
TACCAAACGGTGGCACAGGG+AGG 0.683725 7:+96945776 MsaT040120.1:CDS
TGCGCCGCCATACCAAACGG+TGG 0.683832 7:+96945766 MsaT040120.1:CDS
TGGCAGTGGCACAAACTATG+GGG 0.694881 7:+96945942 MsaT040120.1:CDS
ATTGGAAACGAAAACTCGGG+TGG 0.712637 7:+96946001 MsaT040120.1:CDS
AAAGCAAAAGAGAAACTGAG+AGG 0.716117 7:+96946761 MsaT040120.1:CDS
AATTCTTGCCGCTGTCACAG+AGG 0.718674 7:-96945232 None:intergenic
CTAGGGATAGTATAGGTACG+GGG 0.720083 7:+96945849 MsaT040120.1:CDS
GTTGTTATTAGGATACACTG+CGG 0.806201 7:-96945681 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 96945065 96946814 96945065 ID=MsaG040120
Chr7 mRNA 96945065 96946814 96945065 ID=MsaT040120.1;Parent=MsaG040120
Chr7 exon 96945065 96946162 96945065 ID=MsaT040120.1.exon1;Parent=MsaT040120.1
Chr7 CDS 96945065 96946162 96945065 ID=cds.MsaT040120.1;Parent=MsaT040120.1
Chr7 exon 96946410 96946481 96946410 ID=MsaT040120.1.exon2;Parent=MsaT040120.1
Chr7 CDS 96946410 96946481 96946410 ID=cds.MsaT040120.1;Parent=MsaT040120.1
Chr7 exon 96946579 96946608 96946579 ID=MsaT040120.1.exon3;Parent=MsaT040120.1
Chr7 CDS 96946579 96946608 96946579 ID=cds.MsaT040120.1;Parent=MsaT040120.1
Chr7 exon 96946719 96946814 96946719 ID=MsaT040120.1.exon4;Parent=MsaT040120.1
Chr7 CDS 96946719 96946814 96946719 ID=cds.MsaT040120.1;Parent=MsaT040120.1
Gene Sequence

>MsaT040120.1

ATGGTGGTAAGAGAAGGTGAGATGAATTGGCAACAAGGGGAGGTAGAATCATTGCAGAGGGAAGGAGAAGAAGCGATCAGGAATGACGTCAACAATGTCATTTCTTCATCGCTTGGAAGACAATCGTCATCCATATATTCACTCACCCTCGACGAGTTCCAACACAGCCTCTGTGACAGCGGCAAGAATTTCGGGTCCATGAACATGGATGAGTTTCTAAGTAGTATTTGGAATGCAGAAGAAAACCAACAACAAGCTGCATCCAACAATAATAACAGCAACAACAATAACTTGTCTGCAGCTCAGAAAGGGATTAGCAAACAGGCAAGTCTTCCTCGCCAAAATTCGTTATCGATTCCTGCACCTCTCTGCAGGAAAACTGTTGAACAAGTTTGGTCTGAGATACACAAAGAACAACAGAACCATCACAACATCAACAATGTTGCTCAGAATACCGAGTCTACCCCTCGACAACCGACTTTTGGCGAGATGACATTGGAGGATTTCTTGGTTAAAGCAGGGGTAGTTAGGGAACAACAGTCTGGTATGCCGGTTGCCATAGCACCACCACCTGCCGCAGCAGCAGTGTCATCTCACCGGCCCCAGCAGCATTACGCCGCAGTGTATCCTAATAACAACTCTACAATGGCTCAGGCTGCTTCATTTGCTATTGGCGGCGGCGGCGGGAATCTGAATGTGGTTGCGCCGCCATACCAAACGGTGGCACAGGGAGGTGGAGGAGTTGGAGAGCCTTCGGGTTCGGGGTATGTTGGGAATGGTAAGACTAGGGATAGTATAGGTACGGGGTATCCTCCGCCGCCGCCAGCGGTTTGTTATGGAGGGAGAGTGGTGAATGGAGGGGCGGGCGGTTATGGTGTGGCAGTGGCACAAACTATGGGGATGGGCGGACCTGTTAGTCCGGTTTCTTCGGATGGGATTGGAAACGAAAACTCGGGTGGTCAATTTGGGATTGATATGAATGGTTTAAGAGGAAGAAAGAGGATGGTTGATGGTCCTGTTGAAAGGGTGGTGGAGAGAAGGCAGAGGAGGATGATCAAGAATAGAGAGTCAGCAGCAAGATCTAGAGCCAGAAAACAGGCATACACAGTTGAATTAGAAGCAGAACTGAATCAATTAAGAGAAGAGAATTCTCAACTGAAACAGGCACTGGCTGAACTAGAGAGGAGAAGAAGACAACAGTGTTTAGAGGAAACAAACGTGAGAGTTCAAACCAAAGCTCAGAAAGCAAAAGAGAAACTGAGAGGCTTAAGAAGGAATACAAGTTGTCATATGTGA
Protein sequence

>MsaT040120.1

MVVREGEMNWQQGEVESLQREGEEAIRNDVNNVISSSLGRQSSSIYSLTLDEFQHSLCDSGKNFGSMNMDEFLSSIWNAEENQQQAASNNNNSNNNNLSAAQKGISKQASLPRQNSLSIPAPLCRKTVEQVWSEIHKEQQNHHNINNVAQNTESTPRQPTFGEMTLEDFLVKAGVVREQQSGMPVAIAPPPAAAAVSSHRPQQHYAAVYPNNNSTMAQAASFAIGGGGGNLNVVAPPYQTVAQGGGGVGEPSGSGYVGNGKTRDSIGTGYPPPPPAVCYGGRVVNGGAGGYGVAVAQTMGMGGPVSPVSSDGIGNENSGGQFGIDMNGLRGRKRMVDGPVERVVERRQRRMIKNRESAARSRARKQAYTVELEAELNQLREENSQLKQALAELERRRRQQCLEETNVRVQTKAQKAKEKLRGLRRNTSCHM*