AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040225


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040225 MtrunA17_Chr7g0267581 96.707 334 10 1 1 334 1 333 0.0 672
MsaG040225 MtrunA17_Chr1g0177091 70.393 331 89 6 1 327 1 326 4.08e-156 440
MsaG040225 MtrunA17_Chr8g0352251 47.682 151 58 7 99 231 72 219 8.82e-31 118
MsaG040225 MtrunA17_Chr2g0300261 49.296 142 51 7 115 236 10 150 3.77e-29 112
MsaG040225 MtrunA17_Chr2g0292411 51.923 104 48 1 135 236 80 183 3.27e-28 108
MsaG040225 MtrunA17_Chr3g0103391 48.031 127 51 5 106 221 104 226 3.46e-28 111
MsaG040225 MtrunA17_Chr8g0343451 47.458 118 47 4 122 226 110 225 1.73e-25 104
MsaG040225 MtrunA17_Chr2g0329811 51.000 100 40 3 136 226 147 246 7.72e-24 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040225 AT5G06510.1 45.724 304 112 12 33 332 13 267 1.13e-67 213
MsaG040225 AT5G06510.5 45.724 304 112 12 33 332 13 267 1.13e-67 213
MsaG040225 AT5G06510.2 45.724 304 112 12 33 332 13 267 1.13e-67 213
MsaG040225 AT5G06510.6 45.724 304 112 12 33 332 13 267 1.13e-67 213
MsaG040225 AT3G05690.1 44.410 322 112 18 34 333 18 294 8.74e-61 196
MsaG040225 AT5G06510.3 48.963 241 78 10 96 332 19 218 1.74e-60 193
MsaG040225 AT3G05690.2 44.340 318 118 18 34 333 55 331 5.10e-60 196
MsaG040225 AT5G06510.4 49.268 205 65 9 132 332 3 172 1.04e-49 164
MsaG040225 AT5G06510.7 49.268 205 65 9 132 332 3 172 1.04e-49 164
MsaG040225 AT2G34720.1 50.820 122 46 4 125 233 44 164 1.39e-32 120
MsaG040225 AT1G30500.1 53.846 104 42 1 129 226 53 156 5.05e-31 115
MsaG040225 AT2G34720.2 53.097 113 39 4 134 233 18 129 8.10e-31 114
MsaG040225 AT2G34720.3 53.097 113 39 4 134 233 18 129 8.10e-31 114
MsaG040225 AT1G30500.2 51.852 108 42 2 129 226 53 160 2.54e-30 114
MsaG040225 AT1G72830.1 54.717 106 34 3 134 226 135 239 4.33e-29 114
MsaG040225 AT5G12840.5 44.516 155 67 6 125 267 122 269 5.26e-29 113
MsaG040225 AT5G12840.3 44.516 155 67 6 125 267 122 269 5.26e-29 113
MsaG040225 AT5G12840.1 44.516 155 67 6 125 267 122 269 5.26e-29 113
MsaG040225 AT5G12840.4 44.516 155 67 6 125 267 121 268 5.31e-29 113
MsaG040225 AT5G12840.2 44.516 155 67 6 125 267 121 268 5.31e-29 113
MsaG040225 AT1G72830.2 54.206 107 34 4 134 226 135 240 1.56e-27 110
MsaG040225 AT3G20910.1 43.571 140 66 3 135 263 126 263 9.51e-26 105
MsaG040225 AT1G17590.3 50.495 101 41 3 134 226 133 232 1.05e-25 105
MsaG040225 AT1G17590.2 50.495 101 41 3 134 226 133 232 1.05e-25 105
MsaG040225 AT1G17590.4 50.495 101 41 3 134 226 133 232 1.05e-25 105
MsaG040225 AT1G17590.1 50.495 101 41 3 134 226 133 232 1.05e-25 105
MsaG040225 AT3G14020.2 55.670 97 34 3 134 226 140 231 1.20e-25 104
MsaG040225 AT3G14020.1 55.670 97 34 3 134 226 140 231 1.20e-25 104
MsaG040225 AT1G54160.1 59.211 76 30 1 151 226 164 238 1.66e-23 99.0
MsaG040225 AT1G72830.3 49.474 95 31 2 134 226 135 214 8.70e-20 88.6

Find 92 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTACAGAATCAAGCTTTA+AGG 0.131683 7:+98042933 None:intergenic
TTTGGATCTCAATCCCTTTA+TGG 0.173015 7:-98045674 MsaT040225.1:CDS
ACCTCTGATCCAGAAATATT+TGG 0.179271 7:+98043103 None:intergenic
GGAAGCAGCCCAAATATTTC+TGG 0.235006 7:-98043112 MsaT040225.1:CDS
TCAAAGCGATTCGGGGTATC+AGG 0.251958 7:+98044429 None:intergenic
GAACTCCTTGAATCTGATAT+TGG 0.275281 7:-98043163 MsaT040225.1:CDS
AATCACTTTACCATCTTTCC+AGG 0.307723 7:-98045530 MsaT040225.1:intron
GCACCATGGTGGAGTAGTTT+TGG 0.325147 7:-98045692 MsaT040225.1:CDS
ATCCCAGCTCAAAGCGATTC+GGG 0.326860 7:+98044421 None:intergenic
AATCGCTTTGAGCTGGGATT+TGG 0.348352 7:-98044417 MsaT040225.1:CDS
CCAATGAAAAGGATTTGATT+TGG 0.354007 7:+98045639 None:intergenic
GCCAATGGAGATGGTAAAAC+TGG 0.357725 7:-98043244 MsaT040225.1:CDS
GCCAGCTGCTGTTACCCATT+TGG 0.363280 7:+98042967 None:intergenic
AACTCTTCAAAGGAAACTAA+TGG 0.364196 7:-98043133 MsaT040225.1:CDS
GGCCACCAGACTCTCCATAA+AGG 0.392293 7:+98045660 None:intergenic
TGGATCTTCTGTCCACTCTA+TGG 0.396364 7:-98043029 MsaT040225.1:CDS
GCCACCAGACTCTCCATAAA+GGG 0.396734 7:+98045661 None:intergenic
GACTGCCGACTGCAATGATA+TGG 0.407221 7:+98044452 None:intergenic
AATCCCAGCTCAAAGCGATT+CGG 0.417399 7:+98044420 None:intergenic
CATCATCAGATGTCATGTTA+AGG 0.417508 7:+98044024 None:intergenic
AAAAGACACACTTGAATTTG+AGG 0.417906 7:+98044188 None:intergenic
CCTCTGATCCAGAAATATTT+GGG 0.418372 7:+98043104 None:intergenic
AGAGGAACTGAACATGTGTT+TGG 0.420524 7:-98045566 MsaT040225.1:CDS
CAATCCCTTTATGGAGAGTC+TGG 0.421990 7:-98045665 MsaT040225.1:CDS
GTGTTATCATGCCGACCAAA+TGG 0.422418 7:-98042982 MsaT040225.1:CDS
AAGTTCCAATATCAGATTCA+AGG 0.429838 7:+98043158 None:intergenic
TATGGACTCATCTCAACCTA+TGG 0.435805 7:-98044212 MsaT040225.1:CDS
GGAAGTAAAGTGCATAAATT+CGG 0.437987 7:-98043223 MsaT040225.1:CDS
TTTGTGACCAAGTACAGCTT+TGG 0.439346 7:+98043941 None:intergenic
CTCTCGGTCGCCGCATTGCA+TGG 0.444185 7:+98043302 None:intergenic
TTTGTGGGGCAATTATCATC+TGG 0.452637 7:-98045734 MsaT040225.1:CDS
ATCATCAGATGTCATGTTAA+GGG 0.456801 7:+98044025 None:intergenic
TGGAAAGATGGTAAAGTGAT+TGG 0.466352 7:+98045532 None:intergenic
TGCTTAGCATTCACATAAAT+AGG 0.466962 7:+98043999 None:intergenic
GAGGAACTGAACATGTGTTT+GGG 0.473124 7:-98045565 MsaT040225.1:CDS
CTGAACATGTGTTTGGGAAA+GGG 0.489888 7:-98045559 MsaT040225.1:CDS
TCCCGCGCCAAAGCTGTACT+TGG 0.491975 7:-98043948 MsaT040225.1:CDS
ACCCCGAATCGCTTTGAGCT+GGG 0.493523 7:-98044423 MsaT040225.1:CDS
TACCCCGAATCGCTTTGAGC+TGG 0.494554 7:-98044424 MsaT040225.1:CDS
CCATAAAATTGATCTGCATA+AGG 0.496364 7:+98044230 None:intergenic
AAATTACAAACCTGGAAAGA+TGG 0.499330 7:+98045520 None:intergenic
ACCAAATGGGTAACAGCAGC+TGG 0.501310 7:-98042968 MsaT040225.1:CDS
CATAGGTGGCTCCAATGAAA+AGG 0.504139 7:+98045628 None:intergenic
CACTTGAATTTGAGGTCCAT+AGG 0.510920 7:+98044196 None:intergenic
TGGAACTTTAAACTCTTCAA+AGG 0.511753 7:-98043143 MsaT040225.1:CDS
AGGTGACTAGTTTGTATTCG+AGG 0.519014 7:-98043084 MsaT040225.1:CDS
TGGCGTGACTCGTGCATGTA+GGG 0.520062 7:+98043328 None:intergenic
GCTGGGATTTGGTCAACCTA+TGG 0.529602 7:-98044406 MsaT040225.1:intron
ATGGCGTGACTCGTGCATGT+AGG 0.531463 7:+98043327 None:intergenic
TGTTATCATGCCGACCAAAT+GGG 0.536297 7:-98042981 MsaT040225.1:CDS
TGCAATGATATGGCTGTATG+AGG 0.536424 7:+98044462 None:intergenic
AGCTTTACCGTCAATCACCT+TGG 0.539572 7:-98043049 MsaT040225.1:CDS
CTTAACATGACATCTGATGA+TGG 0.543590 7:-98044023 MsaT040225.1:CDS
GGTGACTAGTTTGTATTCGA+GGG 0.543743 7:-98043083 MsaT040225.1:CDS
ACTGAACATGTGTTTGGGAA+AGG 0.545969 7:-98045560 MsaT040225.1:CDS
AAGAAATCTGCCAATGGAGA+TGG 0.548636 7:-98043253 MsaT040225.1:CDS
ATGCGGCGACCGAGAGGATG+TGG 0.549647 7:-98043295 MsaT040225.1:CDS
CCCAAATATTTCTGGATCAG+AGG 0.555972 7:-98043104 MsaT040225.1:CDS
ATTGCTTAGTTGCAGCAAGT+TGG 0.559108 7:+98045594 None:intergenic
GACATGATCGATGGTGGGCG+TGG 0.559837 7:-98043004 MsaT040225.1:CDS
ACCAAGTACAGCTTTGGCGC+GGG 0.561918 7:+98043947 None:intergenic
AGAAGATCCAAGGTGATTGA+CGG 0.562013 7:+98043042 None:intergenic
GCTGCTGTTACCCATTTGGT+CGG 0.574530 7:+98042971 None:intergenic
ATGGCAGACATGATCGATGG+TGG 0.576851 7:-98043010 MsaT040225.1:CDS
GTCGCCGCATTGCATGGAGA+TGG 0.577807 7:+98043308 None:intergenic
CAAAGCGATTCGGGGTATCA+GGG 0.579590 7:+98044430 None:intergenic
GTTTATCTGAAAGAACATGA+TGG 0.580817 7:-98045770 MsaT040225.1:CDS
CAAATTGATTAAACGTAACA+AGG 0.583050 7:-98043922 MsaT040225.1:intron
CGATCATGTCTGCCATAGAG+TGG 0.586528 7:+98043017 None:intergenic
TCATCAGATGTCATGTTAAG+GGG 0.601001 7:+98044026 None:intergenic
AGTTGGTCAACAGCAGACAT+AGG 0.601111 7:+98045611 None:intergenic
GACCAAGTACAGCTTTGGCG+CGG 0.601436 7:+98043946 None:intergenic
TACAGCCATATCATTGCAGT+CGG 0.605390 7:-98044457 MsaT040225.1:CDS
GTGAATGCTAAGCAGTACAA+TGG 0.608645 7:-98043990 MsaT040225.1:CDS
AGTCAACAATTGCAGTTCAG+TGG 0.610037 7:-98043199 MsaT040225.1:CDS
GCAACTAAGCAATCTGCTAG+AGG 0.619360 7:-98045584 MsaT040225.1:CDS
TGGCAGACATGATCGATGGT+GGG 0.625708 7:-98043009 MsaT040225.1:CDS
TAGAGTGGACAGAAGATCCA+AGG 0.626448 7:+98043032 None:intergenic
CATGCAATGCGGCGACCGAG+AGG 0.626832 7:-98043301 MsaT040225.1:CDS
AACACAAAGAAATCTGCCAA+TGG 0.628709 7:-98043259 MsaT040225.1:CDS
AAACGACCACCACATCCTCT+CGG 0.633569 7:+98043286 None:intergenic
GTGACTAGTTTGTATTCGAG+GGG 0.634644 7:-98043082 MsaT040225.1:CDS
TCCCTTTATGGAGAGTCTGG+TGG 0.654622 7:-98045662 MsaT040225.1:CDS
TCCCAGCTCAAAGCGATTCG+GGG 0.658985 7:+98044422 None:intergenic
TCTATGGCAGACATGATCGA+TGG 0.660420 7:-98043013 MsaT040225.1:CDS
CGGCGACCGAGAGGATGTGG+TGG 0.666958 7:-98043292 MsaT040225.1:CDS
ATTCAGCTGCTGCACCATGG+TGG 0.677437 7:-98045703 MsaT040225.1:CDS
TGGTCAACAGCAGACATAGG+TGG 0.678879 7:+98045614 None:intergenic
CACGCCATCTCCATGCAATG+CGG 0.685964 7:-98043312 MsaT040225.1:CDS
ATAATTCAGCTGCTGCACCA+TGG 0.686059 7:-98045706 MsaT040225.1:CDS
ACAATGGAATCATTAGACGT+CGG 0.715449 7:-98043974 MsaT040225.1:CDS
GATCCAAAACTACTCCACCA+TGG 0.743317 7:+98045689 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 98042943 98045807 98042943 ID=MsaG040225
Chr7 mRNA 98042943 98045807 98042943 ID=MsaT040225.1;Parent=MsaG040225
Chr7 exon 98042943 98043350 98042943 ID=MsaT040225.1.exon5;Parent=MsaT040225.1
Chr7 CDS 98042943 98043350 98042943 ID=cds.MsaT040225.1;Parent=MsaT040225.1
Chr7 exon 98043923 98044063 98043923 ID=MsaT040225.1.exon4;Parent=MsaT040225.1
Chr7 CDS 98043923 98044063 98043923 ID=cds.MsaT040225.1;Parent=MsaT040225.1
Chr7 exon 98044199 98044267 98044199 ID=MsaT040225.1.exon3;Parent=MsaT040225.1
Chr7 CDS 98044199 98044267 98044199 ID=cds.MsaT040225.1;Parent=MsaT040225.1
Chr7 exon 98044407 98044516 98044407 ID=MsaT040225.1.exon2;Parent=MsaT040225.1
Chr7 CDS 98044407 98044516 98044407 ID=cds.MsaT040225.1;Parent=MsaT040225.1
Chr7 exon 98045531 98045807 98045531 ID=MsaT040225.1.exon1;Parent=MsaT040225.1
Chr7 CDS 98045531 98045807 98045531 ID=cds.MsaT040225.1;Parent=MsaT040225.1
Gene Sequence

>MsaT040225.1

ATGGCTATGCAAACTGTTTATCTGAAAGAACATGATGGAATTGCACACAATTTTGTGGGGCAATTATCATCTGGTACTAATAATTCAGCTGCTGCACCATGGTGGAGTAGTTTTGGATCTCAATCCCTTTATGGAGAGTCTGGTGGCCAAATCAAATCCTTTTCATTGGAGCCACCTATGTCTGCTGTTGACCAACTTGCTGCAACTAAGCAATCTGCTAGAGGAACTGAACATGTGTTTGGGAAAGGGCATACCAATCACTTTACCATCTTTCCAGATGATTTTAAAATGTCAGGTGATGCACAAAAGCCTCATACAGCCATATCATTGCAGTCGGCAGTCCCTGATACCCCGAATCGCTTTGAGCTGGGATTTGGTCAACCTATGATCTGTACAAAGTATCCTTATGCAGATCAATTTTATGGACTCATCTCAACCTATGGACCTCAAATTCAAGGACGTATAATGCTGCCCCTTAACATGACATCTGATGATGGACCTATTTATGTGAATGCTAAGCAGTACAATGGAATCATTAGACGTCGGCAGTCCCGCGCCAAAGCTGTACTTGGTCACAAATTGATTAAACGTAACAAGCCCTACATGCACGAGTCACGCCATCTCCATGCAATGCGGCGACCGAGAGGATGTGGTGGTCGTTTCTTAAACACAAAGAAATCTGCCAATGGAGATGGTAAAACTGGAAGTAAAGTGCATAAATTCGGCAGTCAACAATTGCAGTTCAGTGGTTCTCAGAGTTCTGAACTCCTTGAATCTGATATTGGAACTTTAAACTCTTCAAAGGAAACTAATGGAAGCAGCCCAAATATTTCTGGATCAGAGGTGACTAGTTTGTATTCGAGGGGAAATCTTGACAGCTTTACCGTCAATCACCTTGGATCTTCTGTCCACTCTATGGCAGACATGATCGATGGTGGGCGTGGTGTTATCATGCCGACCAAATGGGTAACAGCAGCTGGCAACTGCTGTAACCTTAAAGCTTGA

Protein sequence

>MsaT040225.1

MAMQTVYLKEHDGIAHNFVGQLSSGTNNSAAAPWWSSFGSQSLYGESGGQIKSFSLEPPMSAVDQLAATKQSARGTEHVFGKGHTNHFTIFPDDFKMSGDAQKPHTAISLQSAVPDTPNRFELGFGQPMICTKYPYADQFYGLISTYGPQIQGRIMLPLNMTSDDGPIYVNAKQYNGIIRRRQSRAKAVLGHKLIKRNKPYMHESRHLHAMRRPRGCGGRFLNTKKSANGDGKTGSKVHKFGSQQLQFSGSQSSELLESDIGTLNSSKETNGSSPNISGSEVTSLYSRGNLDSFTVNHLGSSVHSMADMIDGGRGVIMPTKWVTAAGNCCNLKA*