Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG040318 | RHN48923.1 | 97.859 | 467 | 9 | 1 | 1 | 466 | 24 | 490 | 0.0 | 957 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG040318 | sp|Q9SCQ6|IDD2_ARATH | 54.180 | 323 | 118 | 6 | 9 | 310 | 44 | 357 | 4.42e-105 | 323 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG040318 | tr|A0A396H847|A0A396H847_MEDTR | 97.859 | 467 | 9 | 1 | 1 | 466 | 24 | 490 | 0.0 | 957 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG001021 | MsaG040318 | 0.824656 | 1.271237e-53 | 3.167789e-51 |
| MsaG001022 | MsaG040318 | 0.826859 | 3.836071e-54 | 1.016038e-51 |
| MsaG001286 | MsaG040318 | 0.827183 | 3.212781e-54 | 8.586517e-52 |
| MsaG001434 | MsaG040318 | 0.816467 | 9.459445e-52 | 1.897935e-49 |
| MsaG001558 | MsaG040318 | 0.837540 | 8.977199e-57 | 3.246461e-54 |
| MsaG001726 | MsaG040318 | 0.800315 | 2.552866e-48 | 3.476204e-46 |
| MsaG003257 | MsaG040318 | 0.805357 | 2.346898e-49 | 3.587697e-47 |
| MsaG003865 | MsaG040318 | 0.841554 | 8.220618e-58 | 3.370379e-55 |
| MsaG003937 | MsaG040318 | 0.831001 | 3.851451e-55 | 1.147065e-52 |
| MsaG004163 | MsaG040318 | 0.838806 | 4.252692e-57 | 1.599379e-54 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG040318 | MtrunA17_Chr7g0268961 | 97.859 | 467 | 9 | 1 | 1 | 466 | 24 | 490 | 0.0 | 957 |
| MsaG040318 | MtrunA17_Chr1g0191991 | 62.893 | 318 | 88 | 7 | 9 | 314 | 73 | 372 | 6.38e-123 | 368 |
| MsaG040318 | MtrunA17_Chr2g0330171 | 55.667 | 300 | 83 | 5 | 9 | 293 | 56 | 320 | 1.55e-106 | 324 |
| MsaG040318 | MtrunA17_Chr8g0342421 | 53.988 | 326 | 120 | 7 | 9 | 321 | 72 | 380 | 4.94e-104 | 319 |
| MsaG040318 | MtrunA17_Chr7g0263941 | 51.541 | 357 | 124 | 10 | 9 | 351 | 44 | 365 | 1.07e-102 | 312 |
| MsaG040318 | MtrunA17_Chr4g0040571 | 49.722 | 360 | 109 | 8 | 9 | 297 | 49 | 407 | 1.17e-102 | 315 |
| MsaG040318 | MtrunA17_Chr8g0373541 | 85.621 | 153 | 22 | 0 | 9 | 161 | 43 | 195 | 6.62e-102 | 314 |
| MsaG040318 | MtrunA17_Chr1g0185121 | 55.518 | 299 | 117 | 4 | 9 | 302 | 50 | 337 | 3.13e-101 | 310 |
| MsaG040318 | MtrunA17_Chr1g0181381 | 82.822 | 163 | 27 | 1 | 9 | 170 | 48 | 210 | 8.23e-101 | 310 |
| MsaG040318 | MtrunA17_Chr1g0210821 | 82.353 | 153 | 27 | 0 | 9 | 161 | 54 | 206 | 2.14e-98 | 306 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG040318 | AT3G50700.1 | 54.180 | 323 | 118 | 6 | 9 | 310 | 44 | 357 | 4.50e-106 | 323 |
| MsaG040318 | AT1G03840.1 | 85.806 | 155 | 22 | 0 | 9 | 163 | 51 | 205 | 4.04e-100 | 309 |
| MsaG040318 | AT1G03840.2 | 85.161 | 155 | 23 | 0 | 9 | 163 | 49 | 203 | 2.09e-99 | 307 |
| MsaG040318 | AT5G44160.2 | 52.077 | 313 | 112 | 7 | 8 | 291 | 7 | 310 | 7.13e-99 | 303 |
| MsaG040318 | AT5G66730.1 | 84.314 | 153 | 24 | 0 | 9 | 161 | 42 | 194 | 1.40e-98 | 305 |
| MsaG040318 | AT5G44160.1 | 52.244 | 312 | 111 | 7 | 9 | 291 | 47 | 349 | 3.16e-98 | 303 |
| MsaG040318 | AT1G55110.3 | 83.871 | 155 | 24 | 1 | 9 | 162 | 73 | 227 | 2.24e-97 | 300 |
| MsaG040318 | AT1G55110.1 | 83.871 | 155 | 24 | 1 | 9 | 162 | 73 | 227 | 2.24e-97 | 300 |
| MsaG040318 | AT1G55110.2 | 83.871 | 155 | 24 | 1 | 9 | 162 | 73 | 227 | 2.24e-97 | 300 |
| MsaG040318 | AT1G14580.1 | 81.699 | 153 | 28 | 0 | 9 | 161 | 63 | 215 | 2.83e-97 | 301 |
Find 96 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATGGCTGGTGTTAGTGG+CGG | 0.638041 | 7:-99104159 | MsaT040318.1:CDS |
| AAGGCTCACCAGAAAACATG+TGG | 0.716558 | 7:-99105197 | MsaT040318.1:CDS |
| AAGTGCAGATGTGAAGAAAA+AGG | 0.388942 | 7:-99105375 | MsaT040318.1:CDS |
| AATAGATCCTACTGCTGAAG+TGG | 0.580873 | 7:-99105534 | MsaT040318.1:CDS |
| AATCTTCAGTTACACCGAAG+AGG | 0.641075 | 7:-99105431 | MsaT040318.1:CDS |
| AATGCAACCACTTCAGCAGT+AGG | 0.598028 | 7:+99105527 | None:intergenic |
| AATTGTTCAAATTTGATACA+TGG | 0.491018 | 7:-99103991 | MsaT040318.1:CDS |
| ACAATCTTCCATGGAAACTT+AGG | 0.513491 | 7:-99105406 | MsaT040318.1:CDS |
| ACCAGAAAACATGTGGTACT+AGG | 0.442303 | 7:-99105190 | MsaT040318.1:CDS |
| AGATCAACCAACAATGCATG+TGG | 0.667821 | 7:-99104531 | MsaT040318.1:CDS |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 99103810 | 99105576 | 99103810 | ID=MsaG040318 |
| Chr7 | mRNA | 99103810 | 99105576 | 99103810 | ID=MsaT040318.1;Parent=MsaG040318 |
| Chr7 | exon | 99103810 | 99104788 | 99103810 | ID=MsaT040318.1.exon2;Parent=MsaT040318.1 |
| Chr7 | CDS | 99103810 | 99104788 | 99103810 | ID=cds.MsaT040318.1;Parent=MsaT040318.1 |
| Chr7 | exon | 99105155 | 99105576 | 99105155 | ID=MsaT040318.1.exon1;Parent=MsaT040318.1 |
| Chr7 | CDS | 99105155 | 99105576 | 99105155 | ID=cds.MsaT040318.1;Parent=MsaT040318.1 |
| Gene Sequence |
>MsaT040318.1 ATGGATGATGTATTTTATGAAATAGATCCTACTGCTGAAGTGGTTGCATTATCTCCAACAACATTAATGGCAACAAATAGATTTGTGTGTGAGATATGCAACAAGGGATTTCAGAGGGACCAGAATCTTCAGTTACACCGAAGAGGTCACAATCTTCCATGGAAACTTAGGCAAAGAACAAGTGCAGATGTGAAGAAAAAGGTGTATGTGTGCCCTGAGCCATCATGTGTTCACCACAATCCAGCTCGTGCATTAGGTGACCTTACTGGCATCAAAAAACATTTTAGCCGCAAACATGGCGAGAAGAAATGGAAATGTGACAAGTGCTCTAAAAGATACGCTGTTCAATCAGATTGGAAGGCTCACCAGAAAACATGTGGTACTAGGGAATACAAATGTGATTGTGGAACCATATTTTCTAGGAGAGACAGTTTTATAACCCACAGAGCATTCTGTGATGCATTAACTGAAGAAAACAACAGAGTAAACCAAGGATTAACCAGTGGCATGCCACCAAACTTGCAAAGCCAAATGCATGATCCTATCTCTACCATGCCTCTAAAACCTGTTTCCAACACTTCCTCAGAATTAAACAATGACTATGACCCTAAAAATTCACTAAGATCCCCAACTCAAGAACATGCTCCAACATCATTCAGATCAACCAACAATGCATGTGGTGGCATGTTCTCAACTAGTGCAGGTGCACTTTTTGGTGGCCCAAAAACCTTGCCTCCATCTTCTTCAACCCTTCAACTTAGTTCCAATTCCAACAGCTTCAATTACTTCAATGACAATAAAAATGGAGGACTAATTGCTGGCATGGTTCAAATGTCGGCAACCGCTTTACTACAAAAAGCAGCACAAATGGGTGCTACTGCAAGCAATAGTGGGAACTCTTCCATGATGCAGAAAAGCTTTGTTAGTAGCATGGTTAGTCCTAATCATGTTTCTGGTTCTATCATGATGCATCATAACCAAAACCAACCCTCTTATGAACACTTTAATCCACTGCAGCATGGATTATCTAACATGGCTGGTGTTAGTGGCGGAGGAGCATTTACTAACCAATTGTTTCAAAAAGAACAACAAGAAATTTCATTACTGTTTGATAACAATACTAATGTGTCTACAATGAATAATGATATTGGAATGTTTAGTCATGGATTGATGAAGAATGTTGCACAAGAGGTTAGTAATTGTTCAAATTTGATACATGGAAGTGATGTGGCAACAGTCCATGATTTCTTGGGAATTGGAGGTTCAAGCTCAAGTTTGCATGAACCACAGCAACAAAGATTGGAAGCATTAAGTCAACAGAGGTTGGAAATTATGAATAATTTTCATCATCATCTTCCACATGAGGATTCAGCAATGGAGAAATCAATTTGGGATGTTTGA |
| Protein sequence |
>MsaT040318.1 MDDVFYEIDPTAEVVALSPTTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLRQRTSADVKKKVYVCPEPSCVHHNPARALGDLTGIKKHFSRKHGEKKWKCDKCSKRYAVQSDWKAHQKTCGTREYKCDCGTIFSRRDSFITHRAFCDALTEENNRVNQGLTSGMPPNLQSQMHDPISTMPLKPVSNTSSELNNDYDPKNSLRSPTQEHAPTSFRSTNNACGGMFSTSAGALFGGPKTLPPSSSTLQLSSNSNSFNYFNDNKNGGLIAGMVQMSATALLQKAAQMGATASNSGNSSMMQKSFVSSMVSPNHVSGSIMMHHNQNQPSYEHFNPLQHGLSNMAGVSGGGAFTNQLFQKEQQEISLLFDNNTNVSTMNNDIGMFSHGLMKNVAQEVSNCSNLIHGSDVATVHDFLGIGGSSSSLHEPQQQRLEALSQQRLEIMNNFHHHLPHEDSAMEKSIWDV* |
