Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG040344 | KEH19403.1 | 71.429 | 133 | 34 | 2 | 11 | 143 | 18 | 146 | 3.77e-59 | 191 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG040344 | sp|Q9FNG6|C3H51_ARATH | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.83e-45 | 153 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG040344 | tr|A0A072U5J1|A0A072U5J1_MEDTR | 71.429 | 133 | 34 | 2 | 11 | 143 | 18 | 146 | 1.80e-59 | 191 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG040344 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG040344 | MtrunA17_Chr7g0269781 | 51.309 | 191 | 25 | 3 | 1 | 124 | 51 | 240 | 3.84e-55 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG040344 | AT5G06420.1 | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.99e-46 | 153 |
| MsaG040344 | AT5G06420.2 | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.99e-46 | 153 |
| MsaG040344 | AT1G01350.1 | 49.375 | 160 | 31 | 1 | 18 | 127 | 94 | 253 | 1.15e-44 | 149 |
Find 29 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAGGGAAAAGGACTTTC+TGG | 0.207237 | 7:-99317333 | MsaT040344.1:CDS |
| GCGCACACTCTTTGCTTTCT+CGG | 0.276894 | 7:+99317382 | None:intergenic |
| GACCGAGGCGACTACAATTC+CGG | 0.339363 | 7:-99317434 | MsaT040344.1:CDS |
| TGAAGACTCAAAATGAAAGT+TGG | 0.349810 | 7:+99317673 | None:intergenic |
| AAAATGAAAGTTGGGCTTCT+CGG | 0.382812 | 7:+99317682 | None:intergenic |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7:+99317503 | None:intergenic |
| GAAGACTCAAAATGAAAGTT+GGG | 0.391037 | 7:+99317674 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7:-99317501 | MsaT040344.1:CDS |
| ACCGAGGCGACTACAATTCC+GGG | 0.427387 | 7:-99317433 | MsaT040344.1:CDS |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 7:-99317627 | MsaT040344.1:CDS |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.477457 | 7:-99317494 | MsaT040344.1:CDS |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 7:-99317412 | MsaT040344.1:CDS |
| AAAGAGTGTGCGCGCGCTGA+TGG | 0.507062 | 7:-99317372 | MsaT040344.1:CDS |
| TTCAAGGAGACCGGGTATTG+TGG | 0.545327 | 7:-99317485 | MsaT040344.1:CDS |
| CTACAATTCCGGGTGGCAGT+TGG | 0.548426 | 7:-99317423 | MsaT040344.1:CDS |
| ACCCGGAATTGTAGTCGCCT+CGG | 0.550971 | 7:+99317432 | None:intergenic |
| CGCGCTGATGGTTTGTCTGA+AGG | 0.556877 | 7:-99317360 | MsaT040344.1:CDS |
| TTCTTTGTCCAACTGCCACC+CGG | 0.562902 | 7:+99317415 | None:intergenic |
| TGCAAGTTTCTTCACGACCG+AGG | 0.564969 | 7:-99317449 | MsaT040344.1:CDS |
| GAGACCGGGTATTGTGGCTA+CGG | 0.578795 | 7:-99317479 | MsaT040344.1:CDS |
| ATCGCCGTAGCCACAATACC+CGG | 0.586901 | 7:+99317475 | None:intergenic |
| GAAACTCTAATATGAGTCGA+AGG | 0.595574 | 7:+99317545 | None:intergenic |
| ACATGTAAATTACAAACACC+CGG | 0.625741 | 7:+99317292 | None:intergenic |
| GCAAAGACTTCAAGGAGACC+GGG | 0.633254 | 7:-99317493 | MsaT040344.1:CDS |
| ATCCGTGGGTCATACTCACA+CGG | 0.649413 | 7:-99317578 | MsaT040344.1:CDS |
| GTGTGAGTATGACCCACGGA+TGG | 0.652577 | 7:+99317580 | None:intergenic |
| GACCGTGTGAGTATGACCCA+CGG | 0.674066 | 7:+99317576 | None:intergenic |
| ATATGAGTCGAAGGTCTGAG+AGG | 0.697705 | 7:+99317554 | None:intergenic |
| GAGGCGACTACAATTCCGGG+TGG | 0.758896 | 7:-99317430 | MsaT040344.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 99317301 | 99317753 | 99317301 | ID=MsaG040344 |
| Chr7 | mRNA | 99317301 | 99317753 | 99317301 | ID=MsaT040344.1;Parent=MsaG040344 |
| Chr7 | exon | 99317301 | 99317753 | 99317301 | ID=MsaT040344.1.exon1;Parent=MsaT040344.1 |
| Chr7 | CDS | 99317301 | 99317753 | 99317301 | ID=cds.MsaT040344.1;Parent=MsaT040344.1 |
| Gene Sequence |
| Protein sequence |
