AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040393

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040393 MtrunA17_Chr7g0269781 96.942 327 10 0 1 327 1 327 0.0 661
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040393 AT1G01350.1 69.375 320 95 2 8 327 20 336 3.44e-163 459
MsaG040393 AT5G06420.1 68.125 320 96 2 8 327 58 371 3.29e-161 455
MsaG040393 AT5G06420.2 68.125 320 96 2 8 327 58 371 3.29e-161 455
MsaG040393 AT4G01023.1 64.407 59 21 0 258 316 76 134 2.58e-26 104
MsaG040393 AT4G01023.3 65.079 63 21 1 254 316 92 153 5.98e-26 104
MsaG040393 AT4G01023.2 64.407 59 21 0 258 316 95 153 8.42e-26 103

Find 65 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATATTCTTCCTATTAAC+AGG 0.250339 7:-99763723 None:intergenic
TCTTCAGAAAACCTGTTAAT+AGG 0.277151 7:+99763712 MsaT040393.1:CDS
TTGTAATCCTTGCATATATC+TGG 0.290645 7:-99764230 None:intergenic
GTGGGTCACACGGTCCTCTC+AGG 0.313412 7:+99764165 MsaT040393.1:CDS
GATCGAGGTGATTACAAATC+TGG 0.331028 7:+99764299 MsaT040393.1:CDS
TGCAAGGATTACAAGGAAAC+TGG 0.409524 7:+99764239 MsaT040393.1:CDS
AAATGAAAGCTGGGTTTCTC+AGG 0.415855 7:-99763900 None:intergenic
CATTTGTCGAAACCCATTTG+TGG 0.418726 7:+99764460 MsaT040393.1:CDS
CTTGCTTAAGAGCTCTTTCA+CGG 0.431115 7:-99764016 None:intergenic
TGCTTACACTTTGTTGAGAC+AGG 0.433928 7:-99764485 None:intergenic
CTGTGAGCATTGTGCATTGA+AGG 0.436410 7:+99764514 MsaT040393.1:CDS
TAGCAAAGCAACAGCAACTT+TGG 0.436558 7:+99763956 MsaT040393.1:CDS
GTTGAGACAGGATCCACAAA+TGG 0.440701 7:-99764473 None:intergenic
AGAAGACTCGAAATGAAAGC+TGG 0.441893 7:-99763910 None:intergenic
ACACGGTCCTCTCAGGGCTT+CGG 0.450938 7:+99764172 MsaT040393.1:CDS
GTCTGCAATCAGCCTACGCT+TGG 0.462383 7:+99764682 MsaT040393.1:CDS
CAATAATTATACAGATCATA+AGG 0.469416 7:+99764100 MsaT040393.1:CDS
GGTGGCAGATGGAGAAAGAA+TGG 0.469953 7:+99764321 MsaT040393.1:CDS
TGCAGGTGAGGATGCGGAAG+AGG 0.471533 7:+99764379 MsaT040393.1:CDS
CGAGGATGTAAAATTATACA+AGG 0.486690 7:+99764073 MsaT040393.1:CDS
ACATACCTTGTTCAGTCGGC+TGG 0.489348 7:-99763574 None:intergenic
GCAAACAATTGCCAGTGAAA+AGG 0.499122 7:+99764139 MsaT040393.1:CDS
CGACAAATGAAACACGCAAA+TGG 0.499537 7:-99764446 None:intergenic
GAAAGCAAGGAAGATGAGAT+TGG 0.507679 7:+99764355 MsaT040393.1:CDS
TGCAGAGCTCTTCGAAGAGC+CGG 0.511210 7:-99763856 None:intergenic
TTGAGACAGGATCCACAAAT+GGG 0.514543 7:-99764472 None:intergenic
AATTATACAGATCATAAGGC+TGG 0.520545 7:+99764104 MsaT040393.1:CDS
ATCGAGGTGATTACAAATCT+GGG 0.520829 7:+99764300 MsaT040393.1:CDS
GAGCTCTTTCACGGATGGCA+CGG 0.524231 7:-99764007 None:intergenic
AGATATATGCAAGGATTACA+AGG 0.530308 7:+99764232 MsaT040393.1:CDS
TGGGTCACACGGTCCTCTCA+GGG 0.532282 7:+99764166 MsaT040393.1:CDS
CTTAAGAGCTCTTTCACGGA+TGG 0.538525 7:-99764012 None:intergenic
AGTCTCAAGGGAAAGAGTAC+TGG 0.538575 7:+99764047 MsaT040393.1:CDS
GAAAACCAGCCGACTGAACA+AGG 0.548242 7:+99763569 MsaT040393.1:CDS
GTGGCAGATGGAGAAAGAAT+GGG 0.553122 7:+99764322 MsaT040393.1:CDS
TTACAAATCTGGGTGGCAGA+TGG 0.553483 7:+99764310 MsaT040393.1:CDS
GAAGACTCGAAATGAAAGCT+GGG 0.554193 7:-99763909 None:intergenic
AGCTCTTTCACGGATGGCAC+GGG 0.560125 7:-99764006 None:intergenic
GAAACTGGTTACTGTGGGTA+TGG 0.560524 7:+99764254 MsaT040393.1:CDS
TATTGAGAATGAAGATAATG+AGG 0.560776 7:+99763755 MsaT040393.1:CDS
TCATGAGATACGAAAGAAGA+TGG 0.563924 7:+99764720 MsaT040393.1:CDS
ATAGGAAGAATATGAGGAAG+CGG 0.564756 7:+99763730 MsaT040393.1:CDS
GGTGAGGATGCGGAAGAGGA+AGG 0.573050 7:+99764383 MsaT040393.1:CDS
CTGTTAATAGGAAGAATATG+AGG 0.583376 7:+99763724 MsaT040393.1:CDS
GGGATGAAGCTGAGAAAGCA+AGG 0.584805 7:+99764342 MsaT040393.1:CDS
TACAAGGAAACTGGTTACTG+TGG 0.587264 7:+99764248 MsaT040393.1:CDS
ACAATTGCCAGTGAAAAGGC+TGG 0.588175 7:+99764143 MsaT040393.1:CDS
TTATACATACCTTGTTCAGT+CGG 0.588673 7:-99763578 None:intergenic
GATGAGATTGGCTGCAGGTG+AGG 0.590274 7:+99764367 MsaT040393.1:CDS
GAAAAGGCTGGTGGGTCACA+CGG 0.600903 7:+99764155 MsaT040393.1:CDS
AGGAAGATGAGATTGGCTGC+AGG 0.603143 7:+99764362 MsaT040393.1:CDS
ATTGGCTGCAGGTGAGGATG+CGG 0.603342 7:+99764373 MsaT040393.1:CDS
ATTGCCAGTGAAAAGGCTGG+TGG 0.607353 7:+99764146 MsaT040393.1:CDS
GATACGAAAGAAGATGGCTG+AGG 0.623889 7:+99764726 MsaT040393.1:CDS
TTGCCAGTGAAAAGGCTGGT+GGG 0.630138 7:+99764147 MsaT040393.1:CDS
CAAGCGACTGAGAGTCTCAA+GGG 0.630489 7:+99764035 MsaT040393.1:CDS
GCAAGCGACTGAGAGTCTCA+AGG 0.636269 7:+99764034 MsaT040393.1:CDS
GGGAAAGAGTACTGGTTCCG+AGG 0.640397 7:+99764055 MsaT040393.1:CDS
ACAAGGAAACTGGTTACTGT+GGG 0.647981 7:+99764249 MsaT040393.1:CDS
TGTAAGTTTATGCATGATCG+AGG 0.650874 7:+99764284 MsaT040393.1:CDS
TTATCAGCCAGATATATGCA+AGG 0.656472 7:+99764223 MsaT040393.1:CDS
AGAGCTCTTCGAAGAGCCGG+TGG 0.686462 7:-99763853 None:intergenic
GAGGATGTAAAATTATACAA+GGG 0.688297 7:+99764074 MsaT040393.1:CDS
ATGTGAGCCGAAGCCCTGAG+AGG 0.689295 7:-99764179 None:intergenic
GAGGTGATTACAAATCTGGG+TGG 0.758440 7:+99764303 MsaT040393.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 99763542 99764756 99763542 ID=MsaG040393
Chr7 mRNA 99763542 99764756 99763542 ID=MsaT040393.1;Parent=MsaG040393
Chr7 exon 99763542 99763590 99763542 ID=MsaT040393.1.exon1;Parent=MsaT040393.1
Chr7 CDS 99763542 99763590 99763542 ID=cds.MsaT040393.1;Parent=MsaT040393.1
Chr7 exon 99763703 99764535 99763703 ID=MsaT040393.1.exon2;Parent=MsaT040393.1
Chr7 CDS 99763703 99764535 99763703 ID=cds.MsaT040393.1;Parent=MsaT040393.1
Chr7 exon 99764655 99764756 99764655 ID=MsaT040393.1.exon3;Parent=MsaT040393.1
Chr7 CDS 99764655 99764756 99764655 ID=cds.MsaT040393.1;Parent=MsaT040393.1
Gene Sequence

>MsaT040393.1

ATGGAAAATTCTGAGGCCAAATCTGCTGAAAACCAGCCGACTGAACAAGTGTGTAGTTTCTTCAGAAAACCTGTTAATAGGAAGAATATGAGGAAGCGGACTATTGAGAATGAAGATAATGAGGATGACTCGAACAATGAAGAGTCTTTGATGCATGTTCAGAAAAAGAACATGAAGGCTGATAATAAGTTGTTCTTTTCCACCGGCTCTTCGAAGAGCTCTGCATCTGCTAAACTGAATGAAGAACCTGAGAAACCCAGCTTTCATTTCGAGTCTTCTAAAGAGATTCAAGTTCAACATGATAGCAAAGCAACAGCAACTTTGGAGACTGAGACTGATTTTTCAAGAGATGCCCGTGCCATCCGTGAAAGAGCTCTTAAGCAAGCGACTGAGAGTCTCAAGGGAAAGAGTACTGGTTCCGAGGATGTAAAATTATACAAGGGAATCAATAATTATACAGATCATAAGGCTGGATTTCGTAGAGAGCAAACAATTGCCAGTGAAAAGGCTGGTGGGTCACACGGTCCTCTCAGGGCTTCGGCTCACATAAGAGTTTCAGCAAGATTTGATTATCAGCCAGATATATGCAAGGATTACAAGGAAACTGGTTACTGTGGGTATGGTGATTCGTGTAAGTTTATGCATGATCGAGGTGATTACAAATCTGGGTGGCAGATGGAGAAAGAATGGGATGAAGCTGAGAAAGCAAGGAAGATGAGATTGGCTGCAGGTGAGGATGCGGAAGAGGAAGGTGCTAGTTTGAATGATGAAGATGATGATGAAGACGTATTACCATTTGCGTGTTTCATTTGTCGAAACCCATTTGTGGATCCTGTCTCAACAAAGTGTAAGCACTACTTCTGTGAGCATTGTGCATTGAAGCATCATGCGAAGAATAAGAAGTGTTTTGTCTGCAATCAGCCTACGCTTGGCATTTTTAATGTGGCTCATGAGATACGAAAGAAGATGGCTGAGGATAAATAG
Protein sequence

>MsaT040393.1

MENSEAKSAENQPTEQVCSFFRKPVNRKNMRKRTIENEDNEDDSNNEESLMHVQKKNMKADNKLFFSTGSSKSSASAKLNEEPEKPSFHFESSKEIQVQHDSKATATLETETDFSRDARAIRERALKQATESLKGKSTGSEDVKLYKGINNYTDHKAGFRREQTIASEKAGGSHGPLRASAHIRVSARFDYQPDICKDYKETGYCGYGDSCKFMHDRGDYKSGWQMEKEWDEAEKARKMRLAAGEDAEEEGASLNDEDDDEDVLPFACFICRNPFVDPVSTKCKHYFCEHCALKHHAKNKKCFVCNQPTLGIFNVAHEIRKKMAEDK*