AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040456

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040456 MtrunA17_Chr7g0270541 93.638 1006 24 4 1 971 1 1001 0.0 1927
MsaG040456 MtrunA17_Chr1g0194121 74.056 1006 215 8 2 971 8 1003 0.0 1463
MsaG040456 MtrunA17_Chr2g0302791 49.211 1014 442 18 18 970 23 1024 0.0 904
MsaG040456 MtrunA17_Chr1g0162871 32.845 1023 543 26 26 970 46 1002 7.99e-139 441
MsaG040456 MtrunA17_Chr4g0046991 61.290 93 36 0 96 188 58 150 3.49e-33 125
MsaG040456 MtrunA17_Chr8g0360361 66.667 90 30 0 111 200 65 154 1.84e-32 124
MsaG040456 MtrunA17_Chr8g0334671 54.237 118 47 1 75 185 144 261 2.06e-32 129
MsaG040456 MtrunA17_Chr7g0231911 62.500 96 36 0 100 195 138 233 3.47e-32 127
MsaG040456 MtrunA17_Chr2g0308321 72.368 76 21 0 109 184 169 244 2.30e-31 130
MsaG040456 MtrunA17_Chr8g0386271 57.000 100 42 1 96 195 85 183 2.34e-31 127
MsaG040456 MtrunA17_Chr4g0061651 52.000 125 50 2 66 184 76 196 5.42e-31 127
MsaG040456 MtrunA17_Chr5g0420051 66.250 80 27 0 111 190 181 260 5.64e-30 124
MsaG040456 MtrunA17_Chr2g0282521 64.444 90 30 1 96 185 51 138 1.74e-29 114
MsaG040456 MtrunA17_Chr3g0132091 44.961 129 69 1 89 215 51 179 9.26e-29 119
MsaG040456 MtrunA17_Chr7g0258471 67.568 74 24 0 111 184 142 215 1.08e-27 117
MsaG040456 MtrunA17_Chr7g0224821 59.551 89 34 1 96 184 64 150 1.65e-27 115
MsaG040456 MtrunA17_Chr3g0122411 60.000 80 32 0 111 190 165 244 1.11e-25 111
MsaG040456 MtrunA17_Chr2g0286661 25.765 392 228 14 388 731 233 609 4.70e-25 112
MsaG040456 MtrunA17_Chr2g0286661 49.367 79 40 0 111 189 103 181 7.45e-20 95.5
MsaG040456 MtrunA17_Chr8g0377421 47.000 100 53 0 96 195 117 216 3.80e-22 98.2
MsaG040456 MtrunA17_Chr8g0377401 48.315 89 46 0 95 183 146 234 1.03e-19 90.1
MsaG040456 MtrunA17_Chr2g0317041 63.636 55 20 0 103 157 91 145 8.19e-18 81.6
MsaG040456 MtrunA17_Chr1g0175031 51.852 54 26 0 104 157 67 120 1.15e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040456 AT3G60030.1 54.079 993 350 25 10 971 10 927 0.0 940
MsaG040456 AT2G47070.1 53.078 991 325 25 2 971 10 881 0.0 939
MsaG040456 AT2G47070.2 55.621 845 274 19 135 971 1 752 0.0 857
MsaG040456 AT1G20980.1 30.697 1062 569 29 16 970 33 1034 6.48e-128 413
MsaG040456 AT1G76580.1 31.325 581 354 13 394 970 480 1019 5.30e-77 273
MsaG040456 AT1G76580.1 45.408 196 89 5 25 216 40 221 6.21e-38 154
MsaG040456 AT1G76580.2 45.918 196 88 6 25 216 40 221 1.05e-38 155
MsaG040456 AT1G76580.2 35.780 218 136 2 394 611 480 693 1.51e-31 132
MsaG040456 AT1G76580.3 45.918 196 88 6 25 216 40 221 1.11e-38 155
MsaG040456 AT1G76580.3 36.111 216 134 2 394 609 480 691 5.86e-31 130
MsaG040456 AT1G02065.1 50.000 138 57 3 60 189 133 266 5.23e-32 128
MsaG040456 AT2G33810.1 62.069 87 33 0 102 188 45 131 1.13e-31 120
MsaG040456 AT1G53160.3 66.667 81 27 0 110 190 53 133 5.50e-31 120
MsaG040456 AT1G53160.2 60.417 96 36 1 95 190 40 133 5.65e-31 120
MsaG040456 AT1G53160.1 60.417 96 36 1 95 190 40 133 5.65e-31 120
MsaG040456 AT3G15270.1 64.706 85 30 0 110 194 62 146 6.40e-31 120
MsaG040456 AT1G69170.1 64.444 90 31 1 95 184 109 197 3.15e-30 124
MsaG040456 AT3G57920.1 68.750 80 25 0 105 184 53 132 1.99e-28 118
MsaG040456 AT5G50570.1 53.933 89 41 0 102 190 92 180 8.79e-28 116
MsaG040456 AT5G50570.2 53.933 89 41 0 102 190 92 180 8.79e-28 116
MsaG040456 AT5G50670.1 53.933 89 41 0 102 190 92 180 8.79e-28 116
MsaG040456 AT5G50670.2 53.933 89 41 0 102 190 92 180 8.79e-28 116
MsaG040456 AT5G43270.3 55.670 97 43 0 111 207 169 265 1.60e-27 117
MsaG040456 AT5G43270.1 55.670 97 43 0 111 207 169 265 1.60e-27 117
MsaG040456 AT5G43270.2 55.670 97 43 0 111 207 169 265 1.60e-27 117
MsaG040456 AT2G42200.1 67.568 74 24 0 111 184 74 147 4.78e-26 111
MsaG040456 AT1G27370.6 62.162 74 28 0 111 184 176 249 8.31e-26 110
MsaG040456 AT1G27370.1 62.162 74 28 0 111 184 176 249 1.10e-25 111
MsaG040456 AT1G27370.5 62.162 74 28 0 111 184 176 249 1.10e-25 111
MsaG040456 AT1G27370.3 62.162 74 28 0 111 184 176 249 1.10e-25 111
MsaG040456 AT1G27370.2 62.162 74 28 0 111 184 176 249 1.10e-25 111
MsaG040456 AT1G27370.4 62.162 74 28 0 111 184 176 249 1.10e-25 111
MsaG040456 AT1G27370.7 62.162 74 28 0 111 184 176 249 1.10e-25 111
MsaG040456 AT5G18830.2 29.020 255 157 6 392 637 303 542 1.38e-25 114
MsaG040456 AT5G18830.2 51.613 93 43 1 96 188 125 215 1.13e-22 105
MsaG040456 AT5G18830.1 29.020 255 157 6 392 637 303 542 1.69e-25 114
MsaG040456 AT5G18830.1 51.613 93 43 1 96 188 125 215 1.24e-22 104
MsaG040456 AT5G18830.3 29.020 255 157 6 392 637 303 542 1.95e-25 114
MsaG040456 AT5G18830.3 51.613 93 43 1 96 188 125 215 1.40e-22 104
MsaG040456 AT1G27360.2 63.514 74 27 0 111 184 175 248 1.17e-24 107
MsaG040456 AT1G27360.1 63.514 74 27 0 111 184 175 248 1.17e-24 107
MsaG040456 AT1G27360.3 63.514 74 27 0 111 184 175 248 1.17e-24 107
MsaG040456 AT1G27360.4 63.514 74 27 0 111 184 175 248 1.17e-24 107
MsaG040456 AT1G02065.2 42.991 107 49 3 60 158 133 235 1.21e-16 81.3

Find 248 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATACACCCCTTCCTTTC+AGG 0.153174 7:-100399141 MsaT040456.1:CDS
TGTATATATTCCGACCTTTC+AGG 0.184193 7:-100395987 MsaT040456.1:CDS
AGGTGGGAATCTTTGGATTA+TGG 0.220320 7:-100395967 MsaT040456.1:CDS
TTAAAAGAAGGATCTTTATC+TGG 0.221777 7:-100401818 MsaT040456.1:CDS
ACCTTTCAGGTGGGAATCTT+TGG 0.233849 7:-100395974 MsaT040456.1:CDS
ATGGTAATTCCTCTGATTTA+AGG 0.234114 7:-100404240 MsaT040456.1:CDS
TGCACAATTCATCTTTCTTC+TGG 0.235230 7:+100396199 None:intergenic
GATTCTCAGGTTCCCGTAAA+AGG 0.235694 7:+100401843 None:intergenic
TGCTTTAGAAGGGAATTATT+TGG 0.245183 7:-100398861 MsaT040456.1:CDS
TGGAAATGCTGAAGTTAAAA+AGG 0.255582 7:-100396163 MsaT040456.1:CDS
TTCCAGTTTGAATAGGCTTC+TGG 0.255666 7:-100399596 MsaT040456.1:CDS
CATCTTTCCCGGCTGCTATA+TGG 0.271343 7:+100396793 None:intergenic
TTAACCAGTGCCTTGTCTTC+AGG 0.277941 7:+100396879 None:intergenic
CATGGATTTCATTCATGAAA+TGG 0.280274 7:-100397207 MsaT040456.1:CDS
GAAGACAAGGCACTGGTTAA+TGG 0.284075 7:-100396876 MsaT040456.1:CDS
CCTTTCAGGAGTAATAATTA+TGG 0.304697 7:-100399127 MsaT040456.1:CDS
ACTAGGTTTAGGTTTGAATA+AGG 0.311923 7:-100403977 MsaT040456.1:CDS
GGTTCGTTTGAATTCTAGTA+TGG 0.313368 7:-100397147 MsaT040456.1:CDS
AGGTTATTGTGTGCTTTAGA+AGG 0.329096 7:-100398872 MsaT040456.1:CDS
GTTGAGGATGAACTAGGTTT+AGG 0.329278 7:-100403988 MsaT040456.1:CDS
ACAGAAAGCAAATAAAATCT+AGG 0.329856 7:-100397172 MsaT040456.1:CDS
CAGGGACAGAACATGAAAAT+TGG 0.330494 7:+100398774 None:intergenic
GTTTGGCAGGCCATAATAAA+AGG 0.331564 7:-100403542 MsaT040456.1:CDS
TGCTCAGAACAGTTAGATCT+TGG 0.332790 7:-100404057 MsaT040456.1:intron
GGGATTTGAATAGTTGGAAA+TGG 0.337890 7:-100404192 MsaT040456.1:CDS
GCTACATGGGAAGGAAATAA+TGG 0.338198 7:-100403913 MsaT040456.1:CDS
TGAAGGAAGGTGAGAGGAAA+AGG 0.352097 7:-100404023 MsaT040456.1:CDS
TATCTTGCACTTTCAGAGTT+GGG 0.367463 7:-100396993 MsaT040456.1:CDS
TGGTAATTCCTCTGATTTAA+GGG 0.374391 7:-100404239 MsaT040456.1:CDS
CTATTGAACCTCATGCTTGA+TGG 0.376008 7:-100397047 MsaT040456.1:CDS
GCCACCATTGACAGCATTGC+AGG 0.376137 7:+100396066 None:intergenic
TTTCCAGCCCCTTCAACATC+TGG 0.378764 7:+100397251 None:intergenic
GCTTCGCCAGTGGAACTAGA+AGG 0.378856 7:+100401383 None:intergenic
TCATTATACTTACATTCATT+TGG 0.379587 7:-100396304 MsaT040456.1:CDS
TTCCTCTGATTTAAGGGCTA+TGG 0.385188 7:-100404233 MsaT040456.1:CDS
AATCAAGATGCTGTTCCTAA+TGG 0.389375 7:-100403507 MsaT040456.1:CDS
ATCTGACTTCCAGTTTGAAT+AGG 0.389914 7:-100399603 MsaT040456.1:CDS
GTGGTGGTGTTGGTGCTACA+TGG 0.398169 7:-100403927 MsaT040456.1:CDS
TTGGTCTCCAGATTCTTGAT+TGG 0.398756 7:-100399834 MsaT040456.1:intron
CTGATATTGAAGGATATATA+CGG 0.402032 7:-100399795 MsaT040456.1:CDS
GAACTAACCTACTACATTGT+TGG 0.402517 7:+100403719 None:intergenic
AGCCACTTGAATCGATCTAA+TGG 0.406206 7:+100397116 None:intergenic
GAACTGGATGGGTTCACATC+AGG 0.406435 7:-100399549 MsaT040456.1:CDS
TGGCTTGTGTCGAACTTTGT+CGG 0.407195 7:+100396219 None:intergenic
TTTGACTATTTATCTGCGTC+AGG 0.409326 7:-100399758 MsaT040456.1:CDS
AAGAATGGGATTTGAATAGT+TGG 0.409761 7:-100404198 MsaT040456.1:CDS
GGATTTGAATAGTTGGAAAT+GGG 0.410489 7:-100404191 MsaT040456.1:CDS
ATGGATTTCATTCATGAAAT+GGG 0.410942 7:-100397206 MsaT040456.1:CDS
CTGTGTGCTTGAGCCTCTAC+TGG 0.413919 7:-100397291 MsaT040456.1:CDS
TCGTTCGGTGAACTTCCATT+AGG 0.417682 7:+100403492 None:intergenic
ACTCTGAAAGTGCAAGATAC+AGG 0.418841 7:+100396997 None:intergenic
TCCTCTGATTTAAGGGCTAT+GGG 0.419815 7:-100404232 MsaT040456.1:CDS
ATCCAGAAGCCTATTCAAAC+TGG 0.420131 7:+100399594 None:intergenic
TGATTACCCATGGATGCAAC+AGG 0.424656 7:-100401468 MsaT040456.1:CDS
GCTCAGAACAGTTAGATCTT+GGG 0.426023 7:-100404056 MsaT040456.1:intron
CCTGATGCTGTCGGTCCTGC+TGG 0.426837 7:-100396828 MsaT040456.1:CDS
TTGTGCACTACCTTTGAGAT+TGG 0.431132 7:-100396183 MsaT040456.1:CDS
GTATCTTGCACTTTCAGAGT+TGG 0.431765 7:-100396994 MsaT040456.1:CDS
TTTGATGATGGTACAGCAAT+TGG 0.432107 7:+100399085 None:intergenic
TTTCAATTGTACGGTAATGA+TGG 0.435790 7:-100404259 MsaT040456.1:CDS
AAGAAGCTGTCGAAGACGTT+TGG 0.436466 7:-100403559 MsaT040456.1:CDS
ACCTTGTGGCAGGTCGAGTC+AGG 0.438034 7:+100399032 None:intergenic
CTGTCCAGCAGTACTATCTC+TGG 0.440583 7:+100401598 None:intergenic
CAGATCAAAATCATTCATCT+TGG 0.443030 7:+100401574 None:intergenic
ATCCTCAGAACCATCTTTCC+CGG 0.448875 7:+100396782 None:intergenic
TAACTACCGCACACCAATCA+TGG 0.449202 7:+100397075 None:intergenic
CGGAATATATACAGAACTTC+CGG 0.452521 7:+100395997 None:intergenic
TCTGAATTTCCACTTGTTTG+AGG 0.453259 7:+100401425 None:intergenic
CTGAAGCTCATCAAGATCCT+TGG 0.455535 7:+100398802 None:intergenic
CCAGCAGGACCGACAGCATC+AGG 0.458652 7:+100396828 None:intergenic
TAACTTAGGCATTGTCAATC+CGG 0.460858 7:-100396400 MsaT040456.1:intron
TTGCCTGTCACTACAAATCT+TGG 0.461583 7:-100401503 MsaT040456.1:CDS
GATACATTCAAAGGCTAGTA+AGG 0.462061 7:-100403773 MsaT040456.1:CDS
CAGTGGAACTAGAAGGGGAC+TGG 0.466134 7:+100401390 None:intergenic
AGTTCTCTGTGGACCATGAT+TGG 0.466958 7:-100397088 MsaT040456.1:CDS
TAGTTCCACTGGCGAAGCTC+AGG 0.467544 7:-100401378 MsaT040456.1:intron
TATTTATCTGCGTCAGGCTG+AGG 0.471576 7:-100399752 MsaT040456.1:CDS
GAGAAATAGCAGGCAGTTGG+TGG 0.474116 7:-100396949 MsaT040456.1:CDS
TATCCAGATGTTGAAGGGGC+TGG 0.474205 7:-100397254 MsaT040456.1:CDS
GGGAAGAAAAGTAGAGTAGC+TGG 0.477037 7:-100403892 MsaT040456.1:CDS
CAAAATATTTCAGATAAACT+AGG 0.478759 7:-100396903 MsaT040456.1:CDS
TCCTTTCATAGTCGTGGAGG+AGG 0.480311 7:-100397330 MsaT040456.1:CDS
CAGTCCCCTTCTAGTTCCAC+TGG 0.483978 7:-100401389 MsaT040456.1:CDS
ATTATTGTTGAGGATGAACT+AGG 0.485992 7:-100403994 MsaT040456.1:CDS
AAGGAGGGTTATTATTGTTG+AGG 0.486674 7:-100404004 MsaT040456.1:CDS
AGGAAGGTGAGAGGAAAAGG+AGG 0.486683 7:-100404020 MsaT040456.1:CDS
TGAAGATACTCATGAGCCTA+TGG 0.486997 7:-100398834 MsaT040456.1:CDS
AACATGCCAGATTGAGGACC+AGG 0.487015 7:-100397372 MsaT040456.1:intron
GGGGTGTATCAATGACAATC+TGG 0.488591 7:+100399152 None:intergenic
TCGAACTTTGTCGGAATGCT+AGG 0.490308 7:+100396228 None:intergenic
AATCTTAGTTTAAACCTTGG+TGG 0.493064 7:-100403946 MsaT040456.1:CDS
TTGTTTAGACCTGATGCTGT+CGG 0.493104 7:-100396837 MsaT040456.1:CDS
GTCAAATCAGACCGCCGACC+AGG 0.493204 7:-100401936 MsaT040456.1:CDS
CATATAGCAGCCGGGAAAGA+TGG 0.493229 7:-100396792 MsaT040456.1:CDS
GGTTTAGGTTTGAATAAGGA+AGG 0.497519 7:-100403973 MsaT040456.1:CDS
GAAGCCAGAGATAGTACTGC+TGG 0.499262 7:-100401602 MsaT040456.1:CDS
TTCAATATCAGTTGGACTGT+GGG 0.499678 7:+100399807 None:intergenic
CAAGATACCATTGAAAATGA+AGG 0.500592 7:+100399510 None:intergenic
ATTTCATTCATGAAATGGGT+TGG 0.501491 7:-100397202 MsaT040456.1:CDS
TTAAACCTTGGTGGTGGTGT+TGG 0.502647 7:-100403937 MsaT040456.1:CDS
CTACTAGTCTGATCATCGTT+CGG 0.504746 7:+100403477 None:intergenic
CTAGCTCTCTTGATTACCCA+TGG 0.506626 7:-100401478 MsaT040456.1:CDS
CACAGTCCAACTGATATTGA+AGG 0.508704 7:-100399805 MsaT040456.1:CDS
CCCCATAGCCCTTAAATCAG+AGG 0.508712 7:+100404231 None:intergenic
GGTAATCTTAGTTTAAACCT+TGG 0.509670 7:-100403949 MsaT040456.1:CDS
TGTTCTGTCCCTGCGATGAA+TGG 0.511696 7:-100398764 MsaT040456.1:CDS
TAAAAGAAGGATCTTTATCT+GGG 0.513564 7:-100401817 MsaT040456.1:CDS
AAGGCTAGTAAGGCCCTTGT+CGG 0.515263 7:-100403763 MsaT040456.1:CDS
CTACATGGGAAGGAAATAAT+GGG 0.516126 7:-100403912 MsaT040456.1:CDS
AACCCTCGTCCATTCATCGC+AGG 0.517005 7:+100398755 None:intergenic
TGATGCATTCACATGATGCT+AGG 0.517823 7:-100401691 MsaT040456.1:CDS
AGGAGTCTTGCTAATCAGAA+TGG 0.518289 7:-100401893 MsaT040456.1:CDS
TCCTCTTGTACTTAGAGCAC+AGG 0.518622 7:-100399932 MsaT040456.1:intron
GGTTCTGCCAACAATGTAGT+AGG 0.519358 7:-100403726 MsaT040456.1:intron
CCTGAGAATCTGTTAAAAGA+AGG 0.519369 7:-100401830 MsaT040456.1:CDS
GCACCAAGATTTGTAGTGAC+AGG 0.520401 7:+100401500 None:intergenic
TGGAGAACCTTGAGAACCAT+TGG 0.521696 7:+100401766 None:intergenic
AATTATTACTCCTGAAAGGA+AGG 0.522122 7:+100399131 None:intergenic
GTTTCTACTTTAGTTACCAA+TGG 0.523621 7:-100401782 MsaT040456.1:CDS
GCTAATCAGAATGGCGAACA+AGG 0.524392 7:-100401884 MsaT040456.1:CDS
CCATTTAGAATCGCACGGAT+CGG 0.524737 7:-100399993 MsaT040456.1:intron
GATACTTATCCAGATGTTGA+AGG 0.525295 7:-100397260 MsaT040456.1:CDS
GGTTATTGTGTGCTTTAGAA+GGG 0.527744 7:-100398871 MsaT040456.1:CDS
TGTGAATCCTGTTGCATCCA+TGG 0.528171 7:+100401461 None:intergenic
TCCTCCTCCACGACTATGAA+AGG 0.528181 7:+100397329 None:intergenic
AAAAGTAGAGTAGCTGGTGG+AGG 0.528723 7:-100403886 MsaT040456.1:CDS
GGCTGAGGTTGTGTGGGAGG+AGG 0.530578 7:-100399737 MsaT040456.1:intron
TTAGTTACCAATGGTTCTCA+AGG 0.531458 7:-100401773 MsaT040456.1:CDS
CTTAGTTTAAACCTTGGTGG+TGG 0.531572 7:-100403943 MsaT040456.1:CDS
CTTCGCCAGTGGAACTAGAA+GGG 0.533313 7:+100401384 None:intergenic
CAGAAAGCAAATAAAATCTA+GGG 0.536295 7:-100397171 MsaT040456.1:CDS
AGCTGTCGAAGACGTTTGGC+AGG 0.538625 7:-100403555 MsaT040456.1:CDS
GTCCCTGCGATGAATGGACG+AGG 0.539835 7:-100398758 MsaT040456.1:CDS
TTCCATGCTTCAATTCCCAC+CGG 0.539867 7:+100396381 None:intergenic
TTTATCTGGGAAGTCTGAGA+TGG 0.541620 7:-100401804 MsaT040456.1:CDS
GAATTTCCACTTGTTTGAGG+TGG 0.542056 7:+100401428 None:intergenic
ATATATCCTTCAATATCAGT+TGG 0.543073 7:+100399799 None:intergenic
AAAGATGAGTAAGCAGATCC+TGG 0.543962 7:+100401918 None:intergenic
ACACCACTCCATATAGCAGC+CGG 0.544335 7:-100396801 MsaT040456.1:CDS
AACTGGATGGGTTCACATCA+GGG 0.545764 7:-100399548 MsaT040456.1:CDS
TGGTGGTGTTGGTGCTACAT+GGG 0.547732 7:-100403926 MsaT040456.1:CDS
AGTAAAGAATACCTTGTGGC+AGG 0.549712 7:+100399022 None:intergenic
CTCTGAAAGTGCAAGATACA+GGG 0.551934 7:+100396998 None:intergenic
TGGAGGGCTATTTGAAACAC+CGG 0.552422 7:+100401634 None:intergenic
TGCGTCAGGCTGAGGTTGTG+TGG 0.553151 7:-100399744 MsaT040456.1:CDS
AAGAAAATCAACAAGAGTCA+AGG 0.555072 7:-100396276 MsaT040456.1:CDS
GGAAGGTGAGAGGAAAAGGA+GGG 0.555488 7:-100404019 MsaT040456.1:CDS
ACCGGGCTTTGCAGAAAATG+TGG 0.556990 7:+100401652 None:intergenic
AGTACAAGAGGAAAATCGCT+TGG 0.559448 7:+100399943 None:intergenic
CGTAACCGTGCATAATCCTC+AGG 0.560195 7:+100396330 None:intergenic
ATTGAAGGATATATACGGCC+TGG 0.560219 7:-100399790 MsaT040456.1:CDS
TCAGGCTGAGGTTGTGTGGG+AGG 0.560360 7:-100399740 MsaT040456.1:CDS
CATTTCATGAATGAAATCCA+TGG 0.560753 7:+100397208 None:intergenic
ATACTTATCCAGATGTTGAA+GGG 0.561009 7:-100397259 MsaT040456.1:CDS
TCTTGGGATTTGTCAAGTGA+AGG 0.561746 7:-100404040 MsaT040456.1:CDS
GTCAAGTGAAGGAAGGTGAG+AGG 0.564571 7:-100404029 MsaT040456.1:CDS
CTGGCTTCTGAGTAAGCTGG+AGG 0.566231 7:+100401617 None:intergenic
CTTGATGGAACAGTGAGTAC+AGG 0.567632 7:-100397032 MsaT040456.1:CDS
ACCTGTGCTCTAAGTACAAG+AGG 0.568862 7:+100399931 None:intergenic
TTCAGCATTTCCAATCTCAA+AGG 0.571428 7:+100396173 None:intergenic
TCTCTGGCTTCTGAGTAAGC+TGG 0.571429 7:+100401614 None:intergenic
TGGAGTGGTGTCAAACCAGC+AGG 0.572947 7:+100396813 None:intergenic
TCCTTGGAGCATTGATCCAT+AGG 0.573493 7:+100398818 None:intergenic
CCATAATTATTACTCCTGAA+AGG 0.573935 7:+100399127 None:intergenic
TTCTCCTCACAGCTCTGTGA+AGG 0.574334 7:+100396967 None:intergenic
CAGACCTGCAATGCTGTCAA+TGG 0.575070 7:-100396070 MsaT040456.1:CDS
CATCAGGGTGCAGCATCAGA+TGG 0.579332 7:-100399533 MsaT040456.1:CDS
AAATGAGCTGTCTTTGATGA+TGG 0.580500 7:+100399073 None:intergenic
ATCTATATTGACTCAGACGA+TGG 0.581568 7:-100401545 MsaT040456.1:CDS
GTATCACTTGATTCCAGTAG+AGG 0.583315 7:+100397278 None:intergenic
TCCAAAGATTCCCACCTGAA+AGG 0.584115 7:+100395973 None:intergenic
TCGAGTCCACCTCAAACAAG+TGG 0.586688 7:-100401434 MsaT040456.1:CDS
ATTATTACTCCTGAAAGGAA+GGG 0.587708 7:+100399132 None:intergenic
TTAGGTTTGAATAAGGAAGG+TGG 0.588842 7:-100403970 MsaT040456.1:CDS
AAGATTCCCACCTGAAAGGT+CGG 0.589390 7:+100395977 None:intergenic
TTATTACTCCTGAAAGGAAG+GGG 0.590283 7:+100399133 None:intergenic
TTCGCCAGTGGAACTAGAAG+GGG 0.593774 7:+100401385 None:intergenic
TTCCATTAGATCGATTCAAG+TGG 0.595205 7:-100397118 MsaT040456.1:CDS
TTCCCGGCTGCTATATGGAG+TGG 0.595339 7:+100396798 None:intergenic
GGGATTTGTCAAGTGAAGGA+AGG 0.595664 7:-100404036 MsaT040456.1:CDS
CTTCAATATCAGTTGGACTG+TGG 0.596443 7:+100399806 None:intergenic
GCTGCATCGCATTTCCGACA+AGG 0.596748 7:+100403749 None:intergenic
AAGAAAAGTAGAGTAGCTGG+TGG 0.598248 7:-100403889 MsaT040456.1:CDS
AGTGGAACTAGAAGGGGACT+GGG 0.598471 7:+100401391 None:intergenic
GGAGGGCTATTTGAAACACC+GGG 0.598567 7:+100401635 None:intergenic
GAGTCAAGGAGGGGCTCACG+TGG 0.601404 7:-100396262 MsaT040456.1:CDS
TGATTCTGTACTGTAACAGT+TGG 0.603417 7:+100401746 None:intergenic
CTAATCAGAATGGCGAACAA+GGG 0.604164 7:-100401883 MsaT040456.1:CDS
ATGGCATAGAGGACATAGAA+AGG 0.604321 7:-100401526 MsaT040456.1:CDS
ATCCGGTGGGAATTGAAGCA+TGG 0.605101 7:-100396383 MsaT040456.1:CDS
GGTGTTGGTGCTACATGGGA+AGG 0.605894 7:-100403922 MsaT040456.1:CDS
GCGTCAGGCTGAGGTTGTGT+GGG 0.606741 7:-100399743 MsaT040456.1:CDS
GTGAATCCTGTTGCATCCAT+GGG 0.607464 7:+100401462 None:intergenic
AGGACCTGAAGACAAGGCAC+TGG 0.607641 7:-100396883 MsaT040456.1:CDS
CTGCATCGCATTTCCGACAA+GGG 0.607873 7:+100403750 None:intergenic
AGATCGATTCAAGTGGCTCG+TGG 0.608145 7:-100397111 MsaT040456.1:CDS
GAGGAGAAATAGCAGGCAGT+TGG 0.608350 7:-100396952 MsaT040456.1:CDS
GAATGGACGAGGGTTTATTG+AGG 0.611377 7:-100398747 MsaT040456.1:intron
AGTTTGTGAGATACATTCAA+AGG 0.612307 7:-100403782 MsaT040456.1:CDS
CACCACTCCATATAGCAGCC+GGG 0.612629 7:-100396800 MsaT040456.1:CDS
TCTTCTATTCAAAAGCTCGC+CGG 0.612868 7:-100396016 MsaT040456.1:CDS
GAGCTGTGAGGAGAAATAGC+AGG 0.613146 7:-100396959 MsaT040456.1:CDS
CTTAGGCATTGTCAATCCGG+TGG 0.616209 7:-100396397 MsaT040456.1:intron
TTCCACCATTTAGAATCGCA+CGG 0.619213 7:-100399998 MsaT040456.1:intron
GTCAATGGTGGCTATAGCAG+CGG 0.620908 7:-100396055 MsaT040456.1:CDS
ACCTGACTCGACCTGCCACA+AGG 0.620977 7:-100399033 MsaT040456.1:intron
ATATATTCCGACCTTTCAGG+TGG 0.622453 7:-100395984 MsaT040456.1:CDS
ACCCTCGTCCATTCATCGCA+GGG 0.622638 7:+100398756 None:intergenic
TTGTCGGAAATGCGATGCAG+CGG 0.625371 7:-100403747 MsaT040456.1:CDS
TACTTATCCAGATGTTGAAG+GGG 0.625894 7:-100397258 MsaT040456.1:CDS
GCCTATGGATCAATGCTCCA+AGG 0.626151 7:-100398819 MsaT040456.1:CDS
TTAGGCATTGTCAATCCGGT+GGG 0.628619 7:-100396396 MsaT040456.1:intron
TAATCAGAATGGCGAACAAG+GGG 0.629708 7:-100401882 MsaT040456.1:CDS
GAGGATTATGCACGGTTACG+TGG 0.631447 7:-100396327 MsaT040456.1:CDS
CCTCTGATTTAAGGGCTATG+GGG 0.632165 7:-100404231 MsaT040456.1:CDS
TGGCTTCTGAGTAAGCTGGA+GGG 0.633247 7:+100401618 None:intergenic
AAATCAACAAGAGTCAAGGA+GGG 0.635293 7:-100396272 MsaT040456.1:CDS
AAAATCAACAAGAGTCAAGG+AGG 0.635540 7:-100396273 MsaT040456.1:CDS
TGACTCAGACGATGGCATAG+AGG 0.639188 7:-100401537 MsaT040456.1:CDS
TTGCTTCAAGAGTTTGACGA+AGG 0.640841 7:-100403585 MsaT040456.1:CDS
GATGCATTCACATGATGCTA+GGG 0.641130 7:-100401690 MsaT040456.1:CDS
GTGCAAGATACAGGGATGTG+TGG 0.641857 7:+100397006 None:intergenic
TCAAGGAGGGGCTCACGTGG+TGG 0.642540 7:-100396259 MsaT040456.1:CDS
GTGGCTCGTGGAGTTCTCTG+TGG 0.643500 7:-100397099 MsaT040456.1:CDS
AGCCGGGAAAGATGGTTCTG+AGG 0.645502 7:-100396784 MsaT040456.1:CDS
ATGAGTAAGCAGATCCTGGT+CGG 0.645833 7:+100401922 None:intergenic
AAAGCAAATAAAATCTAGGG+TGG 0.646286 7:-100397168 MsaT040456.1:CDS
TAAACTAGGACCTGAAGACA+AGG 0.648850 7:-100396889 MsaT040456.1:CDS
AATCAACAAGAGTCAAGGAG+GGG 0.649223 7:-100396271 MsaT040456.1:CDS
GAATAGTGCACGCGACAGCA+CGG 0.650135 7:-100396361 MsaT040456.1:CDS
CAGCACGGCGTCAACACCTG+AGG 0.654915 7:-100396346 MsaT040456.1:CDS
TCAGGTGAAGGATTGTCGCG+CGG 0.656838 7:-100403842 MsaT040456.1:CDS
AAATACCTGAGCTTCGCCAG+TGG 0.661952 7:+100401373 None:intergenic
ACCTGCAATGCTGTCAATGG+TGG 0.664454 7:-100396067 MsaT040456.1:CDS
ATAGTCAAAACAACACAACC+AGG 0.668652 7:+100399772 None:intergenic
TATATTCCGACCTTTCAGGT+GGG 0.670018 7:-100395983 MsaT040456.1:CDS
AGCAGCGGTCTGTGTCTGTG+TGG 0.670428 7:-100396040 MsaT040456.1:CDS
AGTAAGCAGATCCTGGTCGG+CGG 0.677788 7:+100401925 None:intergenic
TGGCAGGCCATAATAAAAGG+AGG 0.679908 7:-100403539 MsaT040456.1:CDS
TCTCACAAACTTTATGACGT+CGG 0.680002 7:+100403794 None:intergenic
TCACTGTTCCATCAAGCATG+AGG 0.684334 7:+100397039 None:intergenic
ACATGCCAGATTGAGGACCA+GGG 0.686651 7:-100397371 MsaT040456.1:intron
TCCCTGCGATGAATGGACGA+GGG 0.688704 7:-100398757 MsaT040456.1:CDS
GTCTCCTTCACAGAGCTGTG+AGG 0.689109 7:-100396971 MsaT040456.1:CDS
CAACACCTGAGGATTATGCA+CGG 0.704327 7:-100396335 MsaT040456.1:CDS
TAGCACCAACACCACCACCA+AGG 0.720242 7:+100403932 None:intergenic
TGTGGACCATGATTGGTGTG+CGG 0.729884 7:-100397081 MsaT040456.1:CDS
AATCAGAATGGCGAACAAGG+GGG 0.742654 7:-100401881 MsaT040456.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 100395957 100404290 100395957 ID=MsaG040456
Chr7 mRNA 100395957 100404290 100395957 ID=MsaT040456.1;Parent=MsaG040456
Chr7 exon 100395957 100396414 100395957 ID=MsaT040456.1.exon11;Parent=MsaT040456.1
Chr7 CDS 100395957 100396414 100395957 ID=cds.MsaT040456.1;Parent=MsaT040456.1
Chr7 exon 100396754 100397384 100396754 ID=MsaT040456.1.exon10;Parent=MsaT040456.1
Chr7 CDS 100396754 100397384 100396754 ID=cds.MsaT040456.1;Parent=MsaT040456.1
Chr7 exon 100398748 100398892 100398748 ID=MsaT040456.1.exon9;Parent=MsaT040456.1
Chr7 CDS 100398748 100398892 100398748 ID=cds.MsaT040456.1;Parent=MsaT040456.1
Chr7 exon 100399034 100399175 100399034 ID=MsaT040456.1.exon8;Parent=MsaT040456.1
Chr7 CDS 100399034 100399175 100399034 ID=cds.MsaT040456.1;Parent=MsaT040456.1
Chr7 exon 100399518 100399635 100399518 ID=MsaT040456.1.exon7;Parent=MsaT040456.1
Chr7 CDS 100399518 100399635 100399518 ID=cds.MsaT040456.1;Parent=MsaT040456.1
Chr7 exon 100399738 100399845 100399738 ID=MsaT040456.1.exon6;Parent=MsaT040456.1
Chr7 CDS 100399738 100399845 100399738 ID=cds.MsaT040456.1;Parent=MsaT040456.1
Chr7 exon 100399933 100400007 100399933 ID=MsaT040456.1.exon5;Parent=MsaT040456.1
Chr7 CDS 100399933 100400007 100399933 ID=cds.MsaT040456.1;Parent=MsaT040456.1
Chr7 exon 100401379 100401965 100401379 ID=MsaT040456.1.exon4;Parent=MsaT040456.1
Chr7 CDS 100401379 100401965 100401379 ID=cds.MsaT040456.1;Parent=MsaT040456.1
Chr7 exon 100403433 100403614 100403433 ID=MsaT040456.1.exon3;Parent=MsaT040456.1
Chr7 CDS 100403433 100403614 100403433 ID=cds.MsaT040456.1;Parent=MsaT040456.1
Chr7 exon 100403727 100404072 100403727 ID=MsaT040456.1.exon2;Parent=MsaT040456.1
Chr7 CDS 100403727 100404072 100403727 ID=cds.MsaT040456.1;Parent=MsaT040456.1
Chr7 exon 100404167 100404290 100404167 ID=MsaT040456.1.exon1;Parent=MsaT040456.1
Chr7 CDS 100404167 100404290 100404167 ID=cds.MsaT040456.1;Parent=MsaT040456.1
Gene Sequence

>MsaT040456.1

ATGGAAGCTTTTCAATTGTACGGTAATGATGGTAATTCCTCTGATTTAAGGGCTATGGGGAAAAAATCAAAAGAATGGGATTTGAATAGTTGGAAATGGGATAGTGATCTTTTCATAGCTACTAAACAGTTAGATCTTGGGATTTGTCAAGTGAAGGAAGGTGAGAGGAAAAGGAGGGTTATTATTGTTGAGGATGAACTAGGTTTAGGTTTGAATAAGGAAGGTGGTAATCTTAGTTTAAACCTTGGTGGTGGTGTTGGTGCTACATGGGAAGGAAATAATGGGAAGAAAAGTAGAGTAGCTGGTGGAGGTAGTTCTAGTAGAGCATTTTGTCAGGTGAAGGATTGTCGCGCGGATCTTAACAACGCTAAAGATTATCACCGACGTCATAAAGTTTGTGAGATACATTCAAAGGCTAGTAAGGCCCTTGTCGGAAATGCGATGCAGCGGTTCTGCCAACAATGTAGTAGGTTTCACTTGCTTCAAGAGTTTGACGAAGGAAAAAGAAGCTGTCGAAGACGTTTGGCAGGCCATAATAAAAGGAGGAGAAAAACAAATCAAGATGCTGTTCCTAATGGAAGTTCACCGAACGATGATCAGACTAGTAGTTATCTGTTGATAAGTTTATTGAAGATACTTTCAAATATGCAGCCTGACAGGTCAAATCAGACCGCCGACCAGGATCTGCTTACTCATCTTTTAAGGAGTCTTGCTAATCAGAATGGCGAACAAGGGGGAAGGAACTTGTCTAACCTTTTACGGGAACCTGAGAATCTGTTAAAAGAAGGATCTTTATCTGGGAAGTCTGAGATGGTTTCTACTTTAGTTACCAATGGTTCTCAAGGTTCTCCAACTGTTACAGTACAGAATCAAACAGTTTCTATAAGTGAAATTCAGCATCAAGTGATGCATTCACATGATGCTAGGGTTGCTGATCAACAAACCACATTTTCTGCAAAGCCCGGTGTTTCAAATAGCCCTCCAGCTTACTCAGAAGCCAGAGATAGTACTGCTGGACAGACCAAGATGAATGATTTTGATCTGAATGACATCTATATTGACTCAGACGATGGCATAGAGGACATAGAAAGGTTGCCTGTCACTACAAATCTTGGTGCTAGCTCTCTTGATTACCCATGGATGCAACAGGATTCACATCAGTCGAGTCCACCTCAAACAAGTGGAAATTCAGATTCTGCATCTGCCCAGTCCCCTTCTAGTTCCACTGGCGAAGCTCAGAATCGCACGGATCGGATTGTTTTTAAACTCTTCGGTAAAGGACCAAGCGATTTTCCTCTTGTACTTAGAGCACAGATTCTTGATTGGTTATCCCACAGTCCAACTGATATTGAAGGATATATACGGCCTGGTTGTGTTGTTTTGACTATTTATCTGCGTCAGGCTGAGGTTGTGTGGGAGGAGCTATGCTTTGATCTGACTTCCAGTTTGAATAGGCTTCTGGATGTCTCAGATGATGATTTTTGGAGAACTGGATGGGTTCACATCAGGGTGCAGCATCAGATGGCCTTCATTTTCAATGGCCAGATTGTCATTGATACACCCCTTCCTTTCAGGAGTAATAATTATGGTAAGATTTTGAGTGTTAGTCCAATTGCTGTACCATCATCAAAGACAGCTCATTTTTCTGTGAAGGGTATCAACCTGACTCGACCTGCCACAAGGTTATTGTGTGCTTTAGAAGGGAATTATTTGGACTGTGAAGATACTCATGAGCCTATGGATCAATGCTCCAAGGATCTTGATGAGCTTCAGTGCATCCAATTTTCATGTTCTGTCCCTGCGATGAATGGACGAGGGTTTATTGAGATTGAGGACCAGGGTTTGAGCAGCAGTTTTTTTCCTTTCATAGTCGTGGAGGAGGATGTTTGCTCTGAAATCTGTGTGCTTGAGCCTCTACTGGAATCAAGTGATACTTATCCAGATGTTGAAGGGGCTGGAAAAATTCAAGCAAAAAATCAAGCCATGGATTTCATTCATGAAATGGGTTGGCTTCTCCACAGAAAGCAAATAAAATCTAGGGTGGTTCGTTTGAATTCTAGTATGGATCTGTTTCCATTAGATCGATTCAAGTGGCTCGTGGAGTTCTCTGTGGACCATGATTGGTGTGCGGTAGTTAAAAAGCTATTGAACCTCATGCTTGATGGAACAGTGAGTACAGGAGACCACACATCCCTGTATCTTGCACTTTCAGAGTTGGGTCTCCTTCACAGAGCTGTGAGGAGAAATAGCAGGCAGTTGGTGGAGCTTCTTTTGAGATTTGTTCCTCAAAATATTTCAGATAAACTAGGACCTGAAGACAAGGCACTGGTTAATGGAGAGAATCAAAACTTCTTGTTTAGACCTGATGCTGTCGGTCCTGCTGGTTTGACACCACTCCATATAGCAGCCGGGAAAGATGGTTCTGAGGATGTGCTAGATGCTTTGACTAATGATCCTTGCATTGTCAATCCGGTGGGAATTGAAGCATGGAATAGTGCACGCGACAGCACGGCGTCAACACCTGAGGATTATGCACGGTTACGTGGTCATTATACTTACATTCATTTGGTGCAAAAGAAAATCAACAAGAGTCAAGGAGGGGCTCACGTGGTGGTTGATATTCCTAGCATTCCGACAAAGTTCGACACAAGCCAGAAGAAAGATGAATTGTGCACTACCTTTGAGATTGGAAATGCTGAAGTTAAAAAGGTTGGAAAGGACTGCAAACTTTGTGATCATAAGCTATCGTGTAGAACTGCTGTACGAAAGTCTTTCGTATACAGACCTGCAATGCTGTCAATGGTGGCTATAGCAGCGGTCTGTGTCTGTGTGGCTCTTCTATTCAAAAGCTCGCCGGAAGTTCTGTATATATTCCGACCTTTCAGGTGGGAATCTTTGGATTATGGAACAAGCTGA
Protein sequence

>MsaT040456.1

MEAFQLYGNDGNSSDLRAMGKKSKEWDLNSWKWDSDLFIATKQLDLGICQVKEGERKRRVIIVEDELGLGLNKEGGNLSLNLGGGVGATWEGNNGKKSRVAGGGSSSRAFCQVKDCRADLNNAKDYHRRHKVCEIHSKASKALVGNAMQRFCQQCSRFHLLQEFDEGKRSCRRRLAGHNKRRRKTNQDAVPNGSSPNDDQTSSYLLISLLKILSNMQPDRSNQTADQDLLTHLLRSLANQNGEQGGRNLSNLLREPENLLKEGSLSGKSEMVSTLVTNGSQGSPTVTVQNQTVSISEIQHQVMHSHDARVADQQTTFSAKPGVSNSPPAYSEARDSTAGQTKMNDFDLNDIYIDSDDGIEDIERLPVTTNLGASSLDYPWMQQDSHQSSPPQTSGNSDSASAQSPSSSTGEAQNRTDRIVFKLFGKGPSDFPLVLRAQILDWLSHSPTDIEGYIRPGCVVLTIYLRQAEVVWEELCFDLTSSLNRLLDVSDDDFWRTGWVHIRVQHQMAFIFNGQIVIDTPLPFRSNNYGKILSVSPIAVPSSKTAHFSVKGINLTRPATRLLCALEGNYLDCEDTHEPMDQCSKDLDELQCIQFSCSVPAMNGRGFIEIEDQGLSSSFFPFIVVEEDVCSEICVLEPLLESSDTYPDVEGAGKIQAKNQAMDFIHEMGWLLHRKQIKSRVVRLNSSMDLFPLDRFKWLVEFSVDHDWCAVVKKLLNLMLDGTVSTGDHTSLYLALSELGLLHRAVRRNSRQLVELLLRFVPQNISDKLGPEDKALVNGENQNFLFRPDAVGPAGLTPLHIAAGKDGSEDVLDALTNDPCIVNPVGIEAWNSARDSTASTPEDYARLRGHYTYIHLVQKKINKSQGGAHVVVDIPSIPTKFDTSQKKDELCTTFEIGNAEVKKVGKDCKLCDHKLSCRTAVRKSFVYRPAMLSMVAIAAVCVCVALLFKSSPEVLYIFRPFRWESLDYGTS*