AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040476

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040476 MtrunA17_Chr7g0270831 89.183 416 27 7 1 411 31 433 0.0 705
MsaG040476 MtrunA17_Chr4g0075201 42.825 439 190 13 1 380 42 478 3.87e-81 258
MsaG040476 MtrunA17_Chr2g0288871 42.797 472 170 17 1 380 68 531 2.74e-79 254
MsaG040476 MtrunA17_Chr2g0322391 57.269 227 83 4 167 380 332 557 1.81e-72 238
MsaG040476 MtrunA17_Chr3g0099641 39.850 399 181 12 30 378 55 444 2.38e-72 234
MsaG040476 MtrunA17_Chr2g0302391 35.561 374 170 13 62 388 66 415 2.06e-55 189
MsaG040476 MtrunA17_Chr4g0025051 61.146 157 57 2 169 324 320 473 1.54e-54 190
MsaG040476 MtrunA17_Chr4g0024391 45.064 233 106 4 169 382 214 443 4.96e-53 184
MsaG040476 MtrunA17_Chr4g0025061 55.491 173 71 3 155 324 329 498 9.07e-53 186
MsaG040476 MtrunA17_Chr7g0216891 29.847 459 192 10 69 397 45 503 2.18e-48 173
MsaG040476 MtrunA17_Chr4g0051181 40.711 253 112 5 166 381 225 476 2.48e-42 155
MsaG040476 MtrunA17_Chr4g0060271 55.901 161 57 2 158 304 229 389 2.36e-40 151
MsaG040476 MtrunA17_Chr1g0150271 35.266 207 89 7 62 254 6 181 1.44e-31 120
MsaG040476 MtrunA17_Chr2g0333851 29.688 320 155 12 62 375 54 309 1.47e-30 119
MsaG040476 MtrunA17_Chr4g0001361 29.375 320 160 9 65 375 48 310 3.99e-29 115
MsaG040476 MtrunA17_Chr1g0150281 30.631 222 139 3 39 258 6 214 2.87e-26 107
MsaG040476 MtrunA17_Chr5g0419241 85.417 48 7 0 167 214 60 107 1.09e-25 100
MsaG040476 MtrunA17_Chr5g0445311 47.959 98 46 2 156 249 332 428 3.78e-25 107
MsaG040476 MtrunA17_Chr3g0101131 53.086 81 37 1 166 246 397 476 4.77e-24 104
MsaG040476 MtrunA17_Chr7g0218081 52.326 86 40 1 171 256 397 481 1.24e-23 102
MsaG040476 MtrunA17_Chr7g0218081 51.667 60 27 2 185 244 229 286 1.91e-12 68.9
MsaG040476 MtrunA17_Chr3g0136961 53.425 73 34 0 169 241 123 195 1.34e-23 99.8
MsaG040476 MtrunA17_Chr7g0249311 40.000 145 76 5 128 264 328 469 1.35e-23 103
MsaG040476 MtrunA17_Chr7g0249311 50.000 60 28 2 185 244 180 237 4.21e-12 68.2
MsaG040476 MtrunA17_Chr7g0243891 44.068 118 55 2 129 246 329 435 1.87e-23 102
MsaG040476 MtrunA17_Chr7g0270191 36.667 150 69 4 168 297 129 272 2.50e-23 99.0
MsaG040476 MtrunA17_Chr3g0106491 53.247 77 35 1 168 244 349 424 4.31e-23 101
MsaG040476 MtrunA17_Chr3g0106491 50.000 62 29 2 185 246 199 258 1.10e-12 69.7
MsaG040476 MtrunA17_Chr1g0150801 52.703 74 34 1 169 242 87 159 4.85e-23 94.7
MsaG040476 MtrunA17_Chr7g0224801 46.988 83 43 1 173 255 82 163 5.80e-23 95.1
MsaG040476 MtrunA17_Chr3g0079691 50.602 83 40 1 167 249 478 559 7.71e-23 100
MsaG040476 MtrunA17_Chr3g0079691 52.459 61 27 2 185 245 281 339 6.30e-14 73.6
MsaG040476 MtrunA17_Chr4g0004911 53.247 77 35 1 168 244 320 395 1.04e-22 100
MsaG040476 MtrunA17_Chr4g0004911 35.577 104 62 2 154 253 150 252 5.52e-15 76.6
MsaG040476 MtrunA17_Chr5g0418191 46.939 98 51 1 171 268 307 403 1.30e-22 99.4
MsaG040476 MtrunA17_Chr5g0418191 35.238 105 66 2 185 289 152 254 4.67e-13 70.5
MsaG040476 MtrunA17_Chr5g0447701 49.412 85 42 1 169 253 237 320 1.68e-22 98.6
MsaG040476 MtrunA17_Chr5g0447701 35.714 112 63 4 153 261 62 167 9.23e-13 69.3
MsaG040476 MtrunA17_Chr4g0042831 42.727 110 62 1 155 264 249 357 2.46e-22 97.8
MsaG040476 MtrunA17_Chr4g0042831 40.964 83 47 2 185 267 146 226 6.34e-11 63.5
MsaG040476 MtrunA17_Chr2g0294231 50.649 77 37 1 168 244 187 262 4.21e-22 97.1
MsaG040476 MtrunA17_Chr8g0386241 43.902 82 45 1 169 250 88 168 1.02e-21 91.7
MsaG040476 MtrunA17_Chr5g0432161 45.977 87 46 1 168 254 405 490 1.05e-21 97.4
MsaG040476 MtrunA17_Chr5g0432161 50.000 60 28 2 185 244 245 302 1.24e-12 69.7
MsaG040476 MtrunA17_Chr7g0249301 45.556 90 45 2 160 246 265 353 1.20e-21 95.9
MsaG040476 MtrunA17_Chr7g0249301 47.692 65 32 2 180 244 58 120 1.67e-12 68.6
MsaG040476 MtrunA17_Chr4g0051881 47.525 101 41 3 171 266 165 258 2.07e-21 94.4
MsaG040476 MtrunA17_Chr1g0194481 50.649 77 37 1 168 244 142 217 6.54e-21 92.8
MsaG040476 MtrunA17_Chr4g0068541 47.059 85 43 2 168 252 192 274 3.97e-20 91.7
MsaG040476 MtrunA17_Chr8g0383251 45.946 74 39 1 171 244 95 167 6.63e-20 86.7
MsaG040476 MtrunA17_Chr4g0074001 50.633 79 38 1 166 244 341 418 1.36e-19 90.9
MsaG040476 MtrunA17_Chr7g0263531 47.945 73 37 1 172 244 349 420 1.48e-19 90.1
MsaG040476 MtrunA17_Chr7g0263531 50.000 60 28 2 185 244 190 247 8.80e-12 66.6
MsaG040476 MtrunA17_Chr5g0418781 41.228 114 53 3 128 241 81 180 1.91e-19 87.8
MsaG040476 MtrunA17_Chr7g0270051 50.704 71 35 0 182 252 51 121 4.76e-19 86.3
MsaG040476 MtrunA17_Chr8g0379461 50.588 85 40 2 165 248 57 140 5.33e-19 85.9
MsaG040476 MtrunA17_Chr5g0443131 48.649 74 37 1 171 244 144 216 1.48e-18 85.9
MsaG040476 MtrunA17_Chr1g0147881 38.406 138 69 5 120 243 186 321 1.80e-18 85.9
MsaG040476 MtrunA17_Chr3g0129321 42.857 91 47 2 154 244 92 177 2.66e-18 82.8
MsaG040476 MtrunA17_Chr7g0248721 38.000 150 64 7 120 242 50 197 2.73e-18 83.2
MsaG040476 MtrunA17_Chr3g0129361 47.297 74 38 1 168 241 128 200 2.73e-18 83.2
MsaG040476 MtrunA17_Chr2g0284321 54.839 62 28 0 184 245 35 96 4.08e-18 82.8
MsaG040476 MtrunA17_Chr4g0053281 53.226 62 29 0 185 246 267 328 6.50e-18 84.3
MsaG040476 MtrunA17_Chr8g0335811 46.667 75 39 1 167 241 137 210 7.65e-18 82.0
MsaG040476 MtrunA17_Chr1g0184691 52.239 67 32 0 185 251 294 360 1.05e-17 84.0
MsaG040476 MtrunA17_Chr8g0348311 50.000 72 36 0 182 253 153 224 1.09e-17 83.6
MsaG040476 MtrunA17_Chr2g0293481 50.000 70 35 0 184 253 267 336 1.30e-17 84.7
MsaG040476 MtrunA17_Chr2g0319961 44.545 110 51 3 145 244 31 140 1.46e-17 82.0
MsaG040476 MtrunA17_Chr5g0401821 47.297 74 39 0 170 243 185 258 1.50e-17 82.0
MsaG040476 MtrunA17_Chr8g0383351 51.562 64 30 1 178 241 154 216 2.23e-17 80.9
MsaG040476 MtrunA17_Chr7g0248691 50.000 72 36 0 182 253 152 223 6.17e-17 81.3
MsaG040476 MtrunA17_Chr7g0228941 47.887 71 37 0 171 241 39 109 6.75e-17 76.6
MsaG040476 MtrunA17_Chr4g0071521 53.226 62 29 0 180 241 241 302 1.11e-16 80.5
MsaG040476 MtrunA17_Chr1g0188941 48.485 66 33 1 179 244 46 110 2.55e-16 74.7
MsaG040476 MtrunA17_Chr2g0289181 46.970 66 35 0 179 244 9 74 6.84e-16 76.6
MsaG040476 MtrunA17_Chr3g0139021 44.595 74 41 0 168 241 298 371 9.95e-16 78.6
MsaG040476 MtrunA17_Chr8g0387501 35.165 91 59 0 183 273 128 218 2.97e-15 75.5
MsaG040476 MtrunA17_Chr3g0126391 53.333 75 32 2 168 240 118 191 3.11e-15 76.3
MsaG040476 MtrunA17_Chr6g0485431 46.667 75 38 2 171 244 141 214 1.19e-14 74.7
MsaG040476 MtrunA17_Chr1g0202461 45.714 70 38 0 182 251 259 328 1.34e-14 75.1
MsaG040476 MtrunA17_Chr7g0238921 54.688 64 28 1 182 245 122 184 1.34e-14 74.3
MsaG040476 MtrunA17_Chr1g0189391 45.833 72 37 2 171 241 84 154 1.39e-14 74.3
MsaG040476 MtrunA17_Chr8g0347251 36.842 114 69 2 130 240 81 194 8.74e-14 72.0
MsaG040476 MtrunA17_Chr7g0244971 31.250 144 96 2 126 267 66 208 1.06e-13 71.2
MsaG040476 MtrunA17_Chr7g0244981 34.722 144 90 3 126 267 66 207 1.90e-13 70.5
MsaG040476 MtrunA17_Chr8g0387981 50.000 74 34 2 169 240 118 190 2.53e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040476 AT4G01720.1 45.921 429 164 10 39 411 73 489 1.99e-87 274
MsaG040476 AT4G04450.1 39.161 429 173 10 37 378 76 503 2.40e-76 246
MsaG040476 AT4G04450.2 40.657 396 156 9 61 378 15 409 1.60e-75 241
MsaG040476 AT1G62300.1 52.529 257 86 7 167 388 295 550 5.04e-66 220
MsaG040476 AT4G22070.4 71.631 141 38 1 167 305 280 420 3.33e-52 183
MsaG040476 AT4G22070.3 71.631 141 38 1 167 305 280 420 3.33e-52 183
MsaG040476 AT4G22070.2 71.631 141 38 1 167 305 280 420 3.33e-52 183
MsaG040476 AT4G22070.1 71.631 141 38 1 167 305 280 420 3.33e-52 183
MsaG040476 AT1G68150.1 41.518 224 101 5 68 265 97 316 9.32e-47 164
MsaG040476 AT5G15130.2 41.288 264 95 6 168 380 169 423 4.10e-46 166
MsaG040476 AT5G15130.1 41.288 264 95 6 168 380 211 465 4.30e-46 167
MsaG040476 AT1G18860.1 56.291 151 57 2 162 305 219 367 4.02e-40 150
MsaG040476 AT1G69810.2 48.538 171 79 5 150 318 113 276 6.41e-40 145
MsaG040476 AT1G69810.1 48.538 171 79 5 150 318 171 334 1.00e-39 146
MsaG040476 AT1G80840.1 29.023 348 183 12 39 381 9 297 3.57e-29 115
MsaG040476 AT4G31800.1 36.323 223 124 6 54 262 36 254 9.75e-29 114
MsaG040476 AT2G25000.1 33.019 212 112 5 45 249 23 211 9.95e-25 102
MsaG040476 AT2G25000.3 34.031 191 97 4 66 249 22 190 2.38e-24 101
MsaG040476 AT2G25000.2 34.211 190 96 4 67 249 1 168 3.41e-24 100
MsaG040476 AT2G25000.4 34.211 190 96 4 67 249 1 168 3.41e-24 100
MsaG040476 AT4G26640.5 47.253 91 47 1 164 254 185 274 4.48e-24 103
MsaG040476 AT4G26640.5 37.736 106 63 3 175 280 26 128 3.36e-12 68.2
MsaG040476 AT4G26640.6 47.253 91 47 1 164 254 185 274 4.48e-24 103
MsaG040476 AT4G26640.6 37.736 106 63 3 175 280 26 128 3.36e-12 68.2
MsaG040476 AT4G26640.4 47.253 91 47 1 164 254 185 274 4.48e-24 103
MsaG040476 AT4G26640.4 37.736 106 63 3 175 280 26 128 3.36e-12 68.2
MsaG040476 AT4G26640.3 47.253 91 47 1 164 254 185 274 4.48e-24 103
MsaG040476 AT4G26640.3 37.736 106 63 3 175 280 26 128 3.36e-12 68.2
MsaG040476 AT4G26640.1 42.609 115 60 2 145 254 265 378 1.06e-23 103
MsaG040476 AT4G26640.1 37.736 106 63 3 175 280 130 232 4.25e-12 68.2
MsaG040476 AT4G31800.2 46.535 101 49 2 167 262 89 189 1.16e-23 99.4
MsaG040476 AT4G26640.7 42.609 115 60 2 145 254 297 410 1.16e-23 103
MsaG040476 AT4G26640.7 37.736 106 63 3 175 280 162 264 4.63e-12 68.2
MsaG040476 AT4G26640.2 42.609 115 60 2 145 254 337 450 1.60e-23 103
MsaG040476 AT4G26640.2 37.736 106 63 3 175 280 202 304 5.57e-12 67.8
MsaG040476 AT4G18170.1 44.348 115 48 3 145 244 118 231 2.89e-23 99.8
MsaG040476 AT5G13080.1 44.706 85 46 1 171 255 54 137 3.13e-23 95.1
MsaG040476 AT2G38470.1 50.633 79 38 1 168 246 346 423 3.64e-23 102
MsaG040476 AT2G38470.1 50.000 60 28 2 185 244 185 242 1.38e-12 69.7
MsaG040476 AT5G49520.1 51.948 77 36 1 168 244 205 280 4.07e-23 100
MsaG040476 AT1G29860.1 42.241 116 54 4 168 283 120 222 7.89e-23 97.8
MsaG040476 AT5G26170.1 51.389 72 34 1 170 241 99 169 8.17e-23 95.1
MsaG040476 AT2G04880.2 45.882 85 45 1 170 254 269 352 2.20e-22 99.4
MsaG040476 AT2G04880.2 39.175 97 51 3 182 272 109 203 4.21e-13 71.2
MsaG040476 AT2G04880.1 45.882 85 45 1 170 254 293 376 2.99e-22 99.0
MsaG040476 AT2G04880.1 45.070 71 37 2 182 252 109 177 1.66e-12 69.3
MsaG040476 AT1G13960.2 55.405 74 32 1 171 244 243 315 5.37e-22 97.1
MsaG040476 AT1G13960.2 43.820 89 42 4 174 257 61 146 3.13e-13 70.9
MsaG040476 AT1G13960.1 55.405 74 32 1 171 244 396 468 5.60e-22 98.2
MsaG040476 AT1G13960.1 43.820 89 42 4 174 257 214 299 2.07e-13 72.4
MsaG040476 AT5G56270.1 51.250 80 38 1 167 246 470 548 8.72e-22 98.2
MsaG040476 AT5G07100.2 51.948 77 36 1 168 244 125 200 1.11e-21 93.2
MsaG040476 AT5G07100.4 51.948 77 36 1 168 244 125 200 1.11e-21 93.2
MsaG040476 AT5G07100.5 51.948 77 36 1 168 244 125 200 1.11e-21 93.2
MsaG040476 AT5G07100.3 51.948 77 36 1 168 244 125 200 1.11e-21 93.2
MsaG040476 AT2G03340.1 51.899 79 37 1 171 249 402 479 1.35e-21 97.1
MsaG040476 AT2G03340.1 50.000 60 28 2 185 244 251 308 1.68e-12 69.3
MsaG040476 AT5G07100.1 51.948 77 36 1 168 244 218 293 1.63e-21 94.7
MsaG040476 AT5G41570.1 52.000 75 35 1 168 242 82 155 4.56e-21 90.5
MsaG040476 AT3G01970.1 51.351 74 35 1 170 243 51 123 5.84e-21 89.0
MsaG040476 AT1G64000.1 52.778 72 33 1 171 242 101 171 6.32e-21 90.5
MsaG040476 AT2G30250.1 50.633 79 38 1 168 246 312 389 8.57e-21 94.0
MsaG040476 AT5G46350.1 51.351 74 35 1 171 244 170 242 9.61e-21 92.8
MsaG040476 AT2G47260.1 45.918 98 48 2 168 261 158 254 1.93e-20 92.0
MsaG040476 AT4G30935.1 50.633 79 38 1 166 244 313 390 2.52e-20 93.2
MsaG040476 AT3G01080.1 51.351 74 35 1 171 244 293 365 3.92e-20 92.0
MsaG040476 AT3G01080.1 40.659 91 49 3 185 272 168 256 2.82e-12 68.6
MsaG040476 AT3G01080.2 51.351 74 35 1 171 244 331 403 4.46e-20 92.4
MsaG040476 AT3G01080.2 40.659 91 49 3 185 272 206 294 2.90e-12 68.6
MsaG040476 AT2G46130.1 46.237 93 39 2 150 242 6 87 5.24e-20 85.1
MsaG040476 AT4G26440.1 49.367 79 39 1 168 246 356 433 6.24e-20 92.4
MsaG040476 AT4G26440.1 49.180 61 29 2 185 245 179 237 7.31e-12 67.4
MsaG040476 AT1G55600.1 46.835 79 41 1 168 246 291 368 1.41e-19 90.9
MsaG040476 AT3G62340.1 53.247 77 35 1 168 244 102 177 1.85e-19 88.2
MsaG040476 AT1G69310.4 38.835 103 53 2 139 241 111 203 2.29e-19 88.2
MsaG040476 AT1G69310.2 38.835 103 53 2 139 241 111 203 2.29e-19 88.2
MsaG040476 AT1G69310.1 38.835 103 53 2 139 241 111 203 2.29e-19 88.2
MsaG040476 AT2G23320.1 47.619 84 36 1 168 243 216 299 2.64e-19 88.6
MsaG040476 AT2G30590.1 47.126 87 38 1 168 246 289 375 5.98e-19 88.2
MsaG040476 AT1G29280.1 47.500 80 41 1 165 244 56 134 6.36e-19 86.3
MsaG040476 AT5G64810.1 47.887 71 36 1 171 241 97 166 6.59e-19 84.7
MsaG040476 AT5G28650.1 46.341 82 36 1 168 241 238 319 1.37e-18 86.7
MsaG040476 AT3G04670.1 45.122 82 37 1 168 241 238 319 6.03e-18 84.7
MsaG040476 AT4G24240.1 54.688 64 29 0 180 243 277 340 6.45e-18 85.1
MsaG040476 AT4G39410.1 47.297 74 38 1 168 241 207 279 8.68e-18 84.0
MsaG040476 AT5G52830.1 52.778 72 34 0 182 253 163 234 9.38e-18 84.3
MsaG040476 AT3G58710.1 49.383 81 38 1 175 255 62 139 9.39e-18 83.2
MsaG040476 AT3G58710.2 49.383 81 38 1 175 255 61 138 9.52e-18 83.2
MsaG040476 AT2G44745.1 44.186 86 37 2 156 241 127 201 1.02e-17 82.0
MsaG040476 AT2G34830.1 45.783 83 44 1 170 251 200 282 1.33e-17 84.7
MsaG040476 AT2G34830.2 45.783 83 44 1 170 251 209 291 1.47e-17 84.7
MsaG040476 AT4G31800.3 35.714 182 99 6 54 221 36 213 1.63e-17 81.6
MsaG040476 AT1G30650.1 45.783 83 44 1 170 251 202 284 1.92e-17 84.3
MsaG040476 AT2G24570.1 57.895 57 24 0 185 241 244 300 3.45e-17 82.4
MsaG040476 AT2G37260.3 49.333 75 34 2 179 250 264 337 6.72e-17 82.0
MsaG040476 AT2G37260.3 41.935 93 43 4 162 249 67 153 2.74e-13 71.2
MsaG040476 AT2G37260.2 49.333 75 34 2 179 250 262 335 6.79e-17 82.0
MsaG040476 AT2G37260.2 41.935 93 43 4 162 249 65 151 2.84e-13 70.9
MsaG040476 AT4G01250.1 48.611 72 37 0 182 253 126 197 7.88e-17 80.9
MsaG040476 AT4G31550.1 56.140 57 25 0 185 241 247 303 1.09e-16 80.9
MsaG040476 AT2G37260.1 49.333 75 34 2 179 250 344 417 1.12e-16 82.0
MsaG040476 AT2G37260.1 41.935 93 43 4 162 249 147 233 3.74e-13 71.2
MsaG040476 AT4G31550.2 56.140 57 25 0 185 241 246 302 1.18e-16 80.9
MsaG040476 AT2G46130.2 43.011 93 38 3 150 242 6 83 1.73e-16 75.1
MsaG040476 AT4G23550.1 43.210 81 46 0 179 259 135 215 1.90e-16 80.1
MsaG040476 AT5G45050.2 40.000 95 55 1 184 276 1152 1246 3.57e-15 78.6
MsaG040476 AT5G45050.1 40.000 95 55 1 184 276 1180 1274 3.85e-15 78.2
MsaG040476 AT5G45050.4 40.000 95 55 1 184 276 1041 1135 3.89e-15 78.2
MsaG040476 AT1G69310.3 35.922 103 52 3 139 241 111 199 2.28e-14 73.6
MsaG040476 AT2G21900.1 32.231 121 72 3 164 284 92 202 1.45e-13 69.7
MsaG040476 AT5G43290.1 31.159 138 75 5 119 241 38 170 2.51e-13 70.5
MsaG040476 AT3G01080.3 40.659 91 49 3 185 272 206 294 1.55e-12 68.6
MsaG040476 AT5G45260.1 40.860 93 49 2 169 259 1197 1285 1.71e-12 70.1
MsaG040476 AT2G46400.1 44.000 75 40 1 168 240 88 162 1.89e-12 68.2
MsaG040476 AT2G40740.1 46.667 75 35 2 185 254 174 248 4.68e-12 66.6
MsaG040476 AT2G40740.3 46.667 75 35 2 185 254 183 257 6.09e-12 66.6
MsaG040476 AT3G56400.1 34.821 112 70 2 132 240 67 178 7.99e-11 63.2

Find 97 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTAAAACGTTACGTTAAC+AGG 0.198700 7:+100583081 None:intergenic
GCCAAAGCTGTAGTAAAATT+TGG 0.236789 7:+100578389 None:intergenic
GGATGTCAGTGGAGGAAATA+TGG 0.326047 7:-100580312 MsaT040476.1:CDS
GAATAATAATGCATTGGTTT+CGG 0.333185 7:-100580722 MsaT040476.1:CDS
ATGCAACATCCGCAACTACT+AGG 0.336584 7:-100578518 MsaT040476.1:CDS
GAGGAACTCTGGAGAGGTTA+TGG 0.338532 7:+100578558 None:intergenic
TTGTAGTTGGCTATAGCTTT+TGG 0.341519 7:+100582703 None:intergenic
TTTATTACCTGTTTGCGAAC+TGG 0.349172 7:+100580222 None:intergenic
GCAACCACCACCTTCATCTA+TGG 0.378686 7:-100578444 MsaT040476.1:CDS
GCAGTTGCAGCTGGTGGGAG+TGG 0.382339 7:+100578755 None:intergenic
TTGCGTGGGTTCTTCTAACT+TGG 0.393374 7:+100580642 None:intergenic
GGAAGGGTTGAGAAGCTGAT+AGG 0.399322 7:+100578609 None:intergenic
GAATCTTCTGCAGGATGATC+AGG 0.403070 7:+100580676 None:intergenic
GCAGCGATTTCATCAATCAT+TGG 0.419822 7:-100578365 MsaT040476.1:CDS
TTATTACCTGTTTGCGAACT+GGG 0.426338 7:+100580223 None:intergenic
AAAATTTGGATCCGAAGAAA+TGG 0.435237 7:+100578403 None:intergenic
TGTTAGTGCAGCCATTTCTT+CGG 0.448790 7:-100578414 MsaT040476.1:CDS
GATGGTAATAATAATCTTGC+AGG 0.450084 7:-100578326 MsaT040476.1:CDS
TTTGCTATGGCAGTTGCAGC+TGG 0.458465 7:+100578746 None:intergenic
CGATAGTAGGCACGAGGACA+AGG 0.465488 7:+100580267 None:intergenic
AGCTTCTGATCTTGCACGTA+TGG 0.466004 7:+100580588 None:intergenic
TGCCTACTATCGTTGCACTA+TGG 0.467155 7:-100580256 MsaT040476.1:CDS
CAGAATGTGGAAGAAAATCA+TGG 0.469000 7:-100580769 MsaT040476.1:CDS
CAGTTGCAGCTGGTGGGAGT+GGG 0.476377 7:+100578756 None:intergenic
TCTTGAAAATTCTGAAACTG+AGG 0.483824 7:-100582914 MsaT040476.1:intron
AGGATGCCCAGTTCGCAAAC+AGG 0.487149 7:-100580229 MsaT040476.1:intron
TGCAACATCCGCAACTACTA+GGG 0.489367 7:-100578517 MsaT040476.1:CDS
TGCTGATATTGTGTTTGCTA+TGG 0.490920 7:+100578733 None:intergenic
CTTGCAGGTGTAGTTCCTGC+AGG 0.491341 7:-100578311 MsaT040476.1:CDS
CTGGTGGGAGTGGGTGAGTG+TGG 0.492649 7:+100578765 None:intergenic
AACCTCATCAACACTTAGAA+AGG 0.495655 7:-100578684 MsaT040476.1:CDS
TGGTAGAGGAGGAGGAACTC+TGG 0.501730 7:+100578547 None:intergenic
GTACTTGACATTGTTGCATA+GGG 0.502525 7:+100578632 None:intergenic
GAGAATGTAGTGCATGATTG+AGG 0.502863 7:+100578275 None:intergenic
GGTACTTGACATTGTTGCAT+AGG 0.510259 7:+100578631 None:intergenic
GTCGAGGGTGATTGTTGGGA+AGG 0.512231 7:+100578592 None:intergenic
AATAATGCATTGGTTTCGGA+TGG 0.512362 7:-100580718 MsaT040476.1:CDS
ATTGGTTTCGGATGGAAAGC+AGG 0.513871 7:-100580710 MsaT040476.1:CDS
TGCGGATGTTGCATTTGAAA+TGG 0.519053 7:+100578527 None:intergenic
ATACACACCTGACCATGGTC+TGG 0.520004 7:+100582647 None:intergenic
AAATGAATTAGATAAATATC+CGG 0.520203 7:+100578655 None:intergenic
TGCAAGATCAGAAGCTCCCC+TGG 0.525707 7:-100580581 MsaT040476.1:intron
GCTATGGCAGTTGCAGCTGG+TGG 0.526091 7:+100578749 None:intergenic
ATTTGAAATGGTAGAGGAGG+AGG 0.528697 7:+100578539 None:intergenic
TCAAGTCGAGGGTGATTGTT+GGG 0.528867 7:+100578588 None:intergenic
TACCACCATTACTTCAACTA+GGG 0.533321 7:-100578466 MsaT040476.1:CDS
CTACCACCATTACTTCAACT+AGG 0.538586 7:-100578467 MsaT040476.1:CDS
CATGGCAGCATCATCAAAGA+AGG 0.547257 7:-100583135 MsaT040476.1:CDS
GAAGGGTTGAGAAGCTGATA+GGG 0.548942 7:+100578610 None:intergenic
TTCTAACTTGGATGAGTGTG+AGG 0.554307 7:+100580654 None:intergenic
GTCAAGTCGAGGGTGATTGT+TGG 0.556628 7:+100578587 None:intergenic
CTATGGCAGTTGCAGCTGGT+GGG 0.556898 7:+100578750 None:intergenic
TGTTGCATTTGAAATGGTAG+AGG 0.559296 7:+100578533 None:intergenic
TTCCTTTCTAAGTGTTGATG+AGG 0.560288 7:+100578682 None:intergenic
AGCCATAGTGCAACGATAGT+AGG 0.563933 7:+100580254 None:intergenic
GGAAGCTGAGGTGAACCTGC+AGG 0.565063 7:+100578296 None:intergenic
TGAACAACTGAGCTTGTAGT+TGG 0.569201 7:+100582690 None:intergenic
ACACTTAGAAAGGAATCAGC+CGG 0.572173 7:-100578674 MsaT040476.1:CDS
TGCATTTGAAATGGTAGAGG+AGG 0.572439 7:+100578536 None:intergenic
ACACTCTCAACCATAGATGA+AGG 0.575020 7:+100578434 None:intergenic
ACGAGGACAAGGGTTACCCT+TGG 0.579166 7:+100580278 None:intergenic
TCGAGGGTGATTGTTGGGAA+GGG 0.581698 7:+100578593 None:intergenic
TCTGCAGGATGATCAGGAGG+AGG 0.584649 7:+100580682 None:intergenic
GAAAGGAATTAGGTCTTGCG+TGG 0.585330 7:+100580627 None:intergenic
GAGTGTGAGGAATCTTCTGC+AGG 0.593547 7:+100580667 None:intergenic
GGAGCACAAAGAGGTGGTGA+TGG 0.595155 7:-100578344 MsaT040476.1:CDS
AGAATGTGGAAGAAAATCAT+GGG 0.597126 7:-100580768 MsaT040476.1:CDS
GGATTAACCCCTAGTAGTTG+CGG 0.597913 7:+100578509 None:intergenic
TATCGTTGCACTATGGCTGT+AGG 0.598056 7:-100580249 MsaT040476.1:CDS
GGTTGCCCTAGTTGAAGTAA+TGG 0.602040 7:+100578461 None:intergenic
AGCTTCTGTGAGAAGAACAA+TGG 0.606352 7:+100578488 None:intergenic
TGTGGAAGAAAATCATGGGA+TGG 0.610182 7:-100580764 MsaT040476.1:CDS
GTATGCTTGAAAGTGAATTG+AGG 0.610723 7:-100582775 MsaT040476.1:CDS
ATCATTGGAGCACAAAGAGG+TGG 0.623691 7:-100578350 MsaT040476.1:CDS
AAAGGAATTAGGTCTTGCGT+GGG 0.624304 7:+100580628 None:intergenic
TGCTGAGTGAGGAGTAGTGT+TGG 0.633875 7:+100583108 None:intergenic
TCAATCATTGGAGCACAAAG+AGG 0.634714 7:-100578353 MsaT040476.1:CDS
CTGCAGGATGATCAGGAGGA+GGG 0.634928 7:+100580683 None:intergenic
TCTTGCACGTATGGAAACCC+TGG 0.642973 7:+100580597 None:intergenic
TAACAGGATCATGCTGAGTG+AGG 0.647028 7:+100583097 None:intergenic
TGCTTGAAAGTGAATTGAGG+AGG 0.651482 7:-100582772 MsaT040476.1:CDS
TCTTCTGCAGGATGATCAGG+AGG 0.653590 7:+100580679 None:intergenic
GAGGAGGAGGAACTCTGGAG+AGG 0.655992 7:+100578552 None:intergenic
CAAACATACACACCTGACCA+TGG 0.657184 7:+100582642 None:intergenic
TCAACCATAGATGAAGGTGG+TGG 0.660007 7:+100578440 None:intergenic
GCAACATCCGCAACTACTAG+GGG 0.665695 7:-100578516 MsaT040476.1:CDS
TGCCCTAGTTGAAGTAATGG+TGG 0.667028 7:+100578464 None:intergenic
AGATCAGCGATGGATGTCAG+TGG 0.669830 7:-100580323 MsaT040476.1:CDS
GATAGTAGGCACGAGGACAA+GGG 0.677877 7:+100580268 None:intergenic
TCAGCGATGGATGTCAGTGG+AGG 0.680116 7:-100580320 MsaT040476.1:CDS
CTCTCAACCATAGATGAAGG+TGG 0.688697 7:+100578437 None:intergenic
ATACTAATAACAACATACGA+AGG 0.689011 7:-100578791 MsaT040476.1:CDS
GTGCATGATTGAGGAAGCTG+AGG 0.696083 7:+100578284 None:intergenic
GCTTGAAAGTGAATTGAGGA+GGG 0.713652 7:-100582771 MsaT040476.1:CDS
GAAGATGATCAAGATCAACA+TGG 0.728705 7:-100583153 MsaT040476.1:CDS
GTGCAACGATAGTAGGCACG+AGG 0.731065 7:+100580261 None:intergenic
AATGATTGATGAAATCGCTG+CGG 0.740375 7:+100578367 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 100578265 100583214 100578265 ID=MsaG040476
Chr7 mRNA 100578265 100583214 100578265 ID=MsaT040476.1;Parent=MsaG040476
Chr7 exon 100578265 100578840 100578265 ID=MsaT040476.1.exon6;Parent=MsaT040476.1
Chr7 CDS 100578265 100578840 100578265 ID=cds.MsaT040476.1;Parent=MsaT040476.1
Chr7 exon 100580230 100580343 100580230 ID=MsaT040476.1.exon5;Parent=MsaT040476.1
Chr7 CDS 100580230 100580343 100580230 ID=cds.MsaT040476.1;Parent=MsaT040476.1
Chr7 exon 100580582 100580788 100580582 ID=MsaT040476.1.exon4;Parent=MsaT040476.1
Chr7 CDS 100580582 100580788 100580582 ID=cds.MsaT040476.1;Parent=MsaT040476.1
Chr7 exon 100582655 100582801 100582655 ID=MsaT040476.1.exon3;Parent=MsaT040476.1
Chr7 CDS 100582655 100582801 100582655 ID=cds.MsaT040476.1;Parent=MsaT040476.1
Chr7 exon 100582915 100582986 100582915 ID=MsaT040476.1.exon2;Parent=MsaT040476.1
Chr7 CDS 100582915 100582986 100582915 ID=cds.MsaT040476.1;Parent=MsaT040476.1
Chr7 exon 100583095 100583214 100583095 ID=MsaT040476.1.exon1;Parent=MsaT040476.1
Chr7 CDS 100583095 100583214 100583095 ID=cds.MsaT040476.1;Parent=MsaT040476.1
Gene Sequence

>MsaT040476.1

ATGGATTTCTTCTCATCATCACCTAATAAAAACAACAAAGAAGATGATCAAGATCAACATGGCAGCATCATCAAAGAAGGATCACCAACACTACTCCTCACTCAGCATGATCCTGTTAACACTGGACTGAATTTAACGTGTTCAAGTGCTGCAGTTTCAAAAGAAAAAAATCTTGAAAATTCTGAAACTGAGATAAGTATGCTTGAAAGTGAATTGAGGAGGGTGCAAGAAGAGAATCATAAATTGAGAATTATGTTAGAACAAATTACCAAAAGCTATAGCCAACTACAAGCTCAGTTGTTCATCACTTTGCAGAAACAAAAACCAGACCATGGTCAGAATGTGGAAGAAAATCATGGGATGGTATCAGAACAAATTTTTTTGAATAATAATGCATTGGTTTCGGATGGAAAGCAGGCTTGCCCTCCTCCTGATCATCCTGCAGAAGATTCCTCACACTCATCCAAGTTAGAAGAACCCACGCAAGACCTAATTCCTTTCAAAAAAGCCAGGGTTTCCATACGTGCAAGATCAGAAGCTCCCCTGATCAGCGATGGATGTCAGTGGAGGAAATATGGACAAAAAATGGCCAAGGGTAACCCTTGTCCTCGTGCCTACTATCGTTGCACTATGGCTGTAGGATGCCCAGTTCGCAAACAGGTTCAAAGATGTGCAGAAGACAAGACAATACTAATAACAACATACGAAGGCAACCACACTCACCCACTCCCACCAGCTGCAACTGCCATAGCAAACACAATATCAGCAGCTGCAGCAATGCTGCTATCAACTTCAACCTCATCAACACTTAGAAAGGAATCAGCCGGATATTTATCTAATTCATTTCCCTATGCAACAATGTCAAGTACCCTATCAGCTTCTCAACCCTTCCCAACAATCACCCTCGACTTGACACAAAACCATAACCTCTCCAGAGTTCCTCCTCCTCTACCATTTCAAATGCAACATCCGCAACTACTAGGGGTTAATCCATTGTTCTTCTCACAGAAGCTACCACCATTACTTCAACTAGGGCAACCACCACCTTCATCTATGGTTGAGAGTGTTAGTGCAGCCATTTCTTCGGATCCAAATTTTACTACAGCTTTGGCCGCAGCGATTTCATCAATCATTGGAGCACAAAGAGGTGGTGATGGTAATAATAATCTTGCAGGTGTAGTTCCTGCAGGTTCACCTCAGCTTCCTCAATCATGCACTACATTCTCTACTAATTAG
Protein sequence

>MsaT040476.1

MDFFSSSPNKNNKEDDQDQHGSIIKEGSPTLLLTQHDPVNTGLNLTCSSAAVSKEKNLENSETEISMLESELRRVQEENHKLRIMLEQITKSYSQLQAQLFITLQKQKPDHGQNVEENHGMVSEQIFLNNNALVSDGKQACPPPDHPAEDSSHSSKLEEPTQDLIPFKKARVSIRARSEAPLISDGCQWRKYGQKMAKGNPCPRAYYRCTMAVGCPVRKQVQRCAEDKTILITTYEGNHTHPLPPAATAIANTISAAAAMLLSTSTSSTLRKESAGYLSNSFPYATMSSTLSASQPFPTITLDLTQNHNLSRVPPPLPFQMQHPQLLGVNPLFFSQKLPPLLQLGQPPPSSMVESVSAAISSDPNFTTALAAAISSIIGAQRGGDGNNNLAGVVPAGSPQLPQSCTTFSTN*