AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040510 MtrunA17_Chr7g0271251 90.896 692 49 5 1 679 1 691 0.0 1255
MsaG040510 MtrunA17_Chr1g0193321 47.548 734 293 16 2 667 1 710 0.0 579
MsaG040510 MtrunA17_Chr1g0182791 44.118 204 80 5 370 543 260 459 6.63e-38 148
MsaG040510 MtrunA17_Chr7g0262691 42.056 214 89 7 369 550 189 399 3.99e-34 136
MsaG040510 MtrunA17_Chr3g0095391 36.755 302 135 10 242 535 148 401 1.14e-33 134
MsaG040510 MtrunA17_Chr7g0270871 51.825 137 54 5 376 506 152 282 4.36e-29 119
MsaG040510 MtrunA17_Chr1g0153241 57.303 89 35 1 430 518 86 171 9.40e-25 104
MsaG040510 MtrunA17_Chr5g0402031 37.255 204 99 6 438 640 114 289 1.38e-23 102
MsaG040510 MtrunA17_Chr7g0231461 45.000 100 54 1 393 491 260 359 6.14e-20 93.6
MsaG040510 MtrunA17_Chr4g0074161 48.684 76 36 1 431 503 208 283 6.86e-18 86.7
MsaG040510 MtrunA17_Chr1g0190731 47.761 67 35 0 431 497 195 261 4.61e-16 80.5
MsaG040510 MtrunA17_Chr5g0393721 32.710 107 68 1 427 533 222 324 9.54e-14 73.2
MsaG040510 MtrunA17_Chr1g0152131 50.000 64 31 1 431 493 262 325 1.25e-13 73.6
MsaG040510 MtrunA17_Chr1g0207311 40.789 76 40 1 432 502 194 269 1.37e-13 72.0
MsaG040510 MtrunA17_Chr4g0035101 38.462 104 61 2 391 493 107 208 2.03e-13 72.0
MsaG040510 MtrunA17_Chr1g0207301 44.828 58 32 0 445 502 304 361 3.50e-13 71.6
MsaG040510 MtrunA17_Chr3g0144641 43.421 76 40 2 419 493 183 256 5.72e-13 71.2
MsaG040510 MtrunA17_Chr3g0134741 45.902 61 33 0 442 502 242 302 6.34e-13 70.5
MsaG040510 MtrunA17_Chr8g0363921 45.312 64 34 1 431 493 153 216 6.38e-13 69.7
MsaG040510 MtrunA17_Chr5g0415291 40.230 87 47 2 408 493 95 177 7.44e-13 68.9
MsaG040510 MtrunA17_Chr7g0234761 46.875 64 33 1 431 493 319 382 2.15e-12 70.1
MsaG040510 MtrunA17_Chr1g0152651 45.902 61 33 0 442 502 287 347 2.41e-12 68.9
MsaG040510 MtrunA17_Chr8g0339091 46.875 64 33 1 431 493 293 356 2.74e-12 69.7
MsaG040510 MtrunA17_Chr5g0421111 46.875 64 33 1 431 493 367 430 2.98e-12 69.7
MsaG040510 MtrunA17_Chr8g0365941 46.875 64 33 1 431 493 307 370 3.02e-12 69.3
MsaG040510 MtrunA17_Chr3g0131771 45.312 64 34 1 431 493 149 212 9.07e-12 67.0
MsaG040510 MtrunA17_Chr8g0385911 37.662 77 47 1 418 493 134 210 4.28e-11 65.1
MsaG040510 MtrunA17_Chr5g0435021 45.000 60 33 0 432 491 221 280 6.65e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040510 AT1G09530.3 31.911 586 277 19 2 578 1 473 1.35e-48 179
MsaG040510 AT1G09530.2 31.911 586 277 19 2 578 1 473 1.35e-48 179
MsaG040510 AT1G09530.5 31.911 586 277 19 2 578 1 473 1.35e-48 179
MsaG040510 AT1G09530.1 31.911 586 277 19 2 578 1 473 1.35e-48 179
MsaG040510 AT1G09530.4 31.911 586 277 19 2 578 1 473 1.35e-48 179
MsaG040510 AT1G09530.6 31.911 586 277 19 2 578 1 473 1.35e-48 179
MsaG040510 AT2G46970.2 37.262 263 133 10 367 619 130 370 3.21e-31 126
MsaG040510 AT2G46970.3 37.262 263 133 10 367 619 139 379 5.88e-31 125
MsaG040510 AT2G46970.1 37.262 263 133 10 367 619 161 401 6.65e-31 126
MsaG040510 AT2G20180.7 41.398 186 80 6 370 535 155 331 2.84e-29 121
MsaG040510 AT2G20180.1 41.398 186 80 6 370 535 155 331 2.84e-29 121
MsaG040510 AT2G20180.5 41.398 186 80 6 370 535 155 331 2.84e-29 121
MsaG040510 AT2G20180.4 39.901 203 91 7 355 535 209 402 1.19e-28 120
MsaG040510 AT2G20180.6 39.901 203 91 7 355 535 209 402 1.19e-28 120
MsaG040510 AT2G20180.2 39.901 203 91 7 355 535 209 402 1.19e-28 120
MsaG040510 AT2G20180.3 39.901 203 91 7 355 535 209 402 1.19e-28 120
MsaG040510 AT2G20180.8 81.667 60 11 0 431 490 280 339 1.95e-26 111
MsaG040510 AT4G28800.1 44.218 147 66 6 422 562 242 378 2.68e-25 110
MsaG040510 AT4G36930.1 67.105 76 25 0 435 510 197 272 5.80e-24 104
MsaG040510 AT4G28815.2 48.485 99 47 2 419 516 58 153 6.62e-23 98.6
MsaG040510 AT5G67110.1 65.333 75 24 2 432 505 90 163 6.96e-23 97.8
MsaG040510 AT4G28800.2 42.857 147 62 6 422 562 254 384 9.03e-23 102
MsaG040510 AT4G28790.1 45.000 120 54 3 422 531 264 381 9.78e-23 101
MsaG040510 AT4G28815.1 48.485 99 47 2 419 516 134 229 3.34e-22 98.2
MsaG040510 AT3G59060.1 44.720 161 65 5 413 566 237 380 4.24e-22 100
MsaG040510 AT3G59060.3 44.720 161 65 5 413 566 237 380 4.90e-22 100
MsaG040510 AT3G59060.2 44.720 161 65 5 413 566 237 380 4.90e-22 100
MsaG040510 AT3G59060.4 44.720 161 65 5 413 566 237 380 4.90e-22 100
MsaG040510 AT4G00050.2 49.485 97 39 2 400 492 78 168 3.53e-21 95.1
MsaG040510 AT4G00050.1 49.485 97 39 2 400 492 180 270 1.19e-20 95.1
MsaG040510 AT4G00050.3 49.485 97 39 2 400 492 180 270 1.45e-20 94.7
MsaG040510 AT2G43010.2 81.667 60 11 0 432 491 254 313 7.17e-20 93.2
MsaG040510 AT2G43010.1 81.667 60 11 0 432 491 254 313 7.43e-20 93.2
MsaG040510 AT2G43010.4 81.667 60 11 0 432 491 254 313 8.31e-20 92.8
MsaG040510 AT2G43010.3 81.667 60 11 0 432 491 261 320 8.76e-20 93.2
MsaG040510 AT2G43010.5 81.667 60 11 0 432 491 296 355 1.29e-19 92.8
MsaG040510 AT4G28790.2 58.824 68 28 0 422 489 264 331 1.05e-18 88.6
MsaG040510 AT2G24260.2 53.731 67 31 0 431 497 37 103 1.05e-18 86.7
MsaG040510 AT3G62090.1 42.017 119 58 2 432 548 168 277 2.55e-18 87.4
MsaG040510 AT2G24260.1 55.556 63 28 0 431 493 140 202 3.93e-18 87.0
MsaG040510 AT3G62090.2 42.017 119 58 2 432 548 185 294 4.35e-18 87.0
MsaG040510 AT5G58010.1 52.239 67 32 0 431 497 101 167 6.98e-18 85.5
MsaG040510 AT4G30980.1 52.239 67 32 0 431 497 132 198 7.07e-18 85.5
MsaG040510 AT1G02340.1 36.364 165 71 7 390 541 107 250 1.14e-17 84.7
MsaG040510 AT1G02340.2 36.364 165 71 7 390 541 100 243 1.21e-17 84.3
MsaG040510 AT4G30980.2 52.239 67 32 0 431 497 180 246 1.96e-17 85.1
MsaG040510 AT4G28811.1 56.250 80 34 1 414 492 335 414 7.55e-14 75.1
MsaG040510 AT1G10120.2 50.000 66 32 1 429 493 206 271 1.28e-13 73.6
MsaG040510 AT1G10120.1 50.000 66 32 1 429 493 206 271 1.28e-13 73.6
MsaG040510 AT1G27740.1 32.031 128 80 3 386 510 128 251 1.56e-13 71.6
MsaG040510 AT1G68920.3 29.469 207 129 6 290 493 175 367 1.91e-13 73.6
MsaG040510 AT3G23690.1 33.577 137 82 4 361 493 125 256 2.03e-13 72.8
MsaG040510 AT5G61270.2 47.170 106 50 4 401 505 47 147 3.27e-13 71.2
MsaG040510 AT5G61270.3 47.170 106 50 4 401 505 47 147 3.27e-13 71.2
MsaG040510 AT2G14760.1 39.474 76 46 0 427 502 235 310 5.66e-13 71.2
MsaG040510 AT4G33880.1 46.032 63 34 0 442 504 279 341 6.05e-13 71.2
MsaG040510 AT5G61270.1 51.899 79 37 1 427 505 158 235 7.21e-13 71.2
MsaG040510 AT2G14760.3 44.615 65 36 0 438 502 259 323 7.60e-13 70.9
MsaG040510 AT5G50915.3 45.312 64 34 1 431 493 138 201 7.67e-13 69.7
MsaG040510 AT5G50915.4 45.312 64 34 1 431 493 138 201 7.67e-13 69.7
MsaG040510 AT5G43175.1 37.647 85 48 2 431 510 132 216 9.28e-13 68.6
MsaG040510 AT5G50915.1 45.312 64 34 1 431 493 138 201 1.31e-12 69.3
MsaG040510 AT5G50915.2 45.312 64 34 1 431 493 138 201 1.31e-12 69.3
MsaG040510 AT1G59640.1 45.312 64 34 1 431 493 138 201 1.47e-12 68.9
MsaG040510 AT3G07340.2 46.875 64 33 1 431 493 260 323 1.86e-12 70.5
MsaG040510 AT1G68920.1 28.502 207 132 5 290 493 175 368 1.92e-12 70.5
MsaG040510 AT1G68920.4 28.502 207 132 5 290 493 175 368 1.92e-12 70.5
MsaG040510 AT1G68920.2 28.502 207 132 5 290 493 175 368 1.92e-12 70.5
MsaG040510 AT1G26260.3 45.455 66 35 1 429 493 172 237 2.30e-12 69.3
MsaG040510 AT3G07340.1 46.875 64 33 1 431 493 260 323 2.38e-12 70.1
MsaG040510 AT1G26260.5 45.455 66 35 1 429 493 201 266 2.38e-12 69.7
MsaG040510 AT1G26260.4 45.455 66 35 1 429 493 223 288 2.70e-12 69.7
MsaG040510 AT1G26260.1 45.455 66 35 1 429 493 223 288 2.70e-12 69.7
MsaG040510 AT1G26260.6 45.455 66 35 1 429 493 223 288 2.70e-12 69.7
MsaG040510 AT1G26260.2 45.455 66 35 1 429 493 223 288 2.70e-12 69.7
MsaG040510 AT5G48560.2 46.875 64 33 1 431 493 303 366 2.71e-12 70.1
MsaG040510 AT5G48560.1 46.875 64 33 1 431 493 303 366 3.12e-12 70.1
MsaG040510 AT1G18400.2 45.312 64 34 1 431 493 147 210 3.53e-12 67.4
MsaG040510 AT1G18400.1 45.312 64 34 1 431 493 147 210 4.60e-12 67.4
MsaG040510 AT4G34530.1 43.750 64 35 1 431 493 174 237 4.94e-12 68.2
MsaG040510 AT1G25330.1 41.975 81 42 2 414 493 93 169 5.36e-12 66.6
MsaG040510 AT1G59640.2 45.312 64 34 1 431 493 138 201 6.25e-12 68.2
MsaG040510 AT5G62610.2 45.312 64 34 1 431 493 155 218 6.66e-12 67.0
MsaG040510 AT5G62610.1 45.312 64 34 1 431 493 155 218 6.66e-12 67.0
MsaG040510 AT1G68240.1 30.128 156 87 5 404 558 47 181 1.78e-11 63.9
MsaG040510 AT4G36540.1 42.188 64 36 1 431 493 143 206 3.14e-11 65.5
MsaG040510 AT4G36540.2 42.188 64 36 1 431 493 143 206 3.23e-11 65.5
MsaG040510 AT1G68240.2 31.655 139 78 4 404 541 47 169 4.46e-11 62.8
MsaG040510 AT1G73830.2 40.625 64 37 1 431 493 149 212 8.16e-11 63.5
MsaG040510 AT2G18300.2 40.625 64 37 1 431 493 187 250 8.70e-11 64.3
MsaG040510 AT2G18300.1 40.625 64 37 1 431 493 187 250 8.74e-11 64.3
MsaG040510 AT1G73830.1 40.625 64 37 1 431 493 149 212 8.90e-11 63.5

Find 133 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTGAAACAAATCGTTTC+TGG 0.196902 7:+101002959 None:intergenic
CAAACTTCCAATCAAGTTCT+TGG 0.266868 7:-101002012 MsaT040510.1:CDS
AGCCCAAACAATTGAGGATT+TGG 0.271198 7:+100994202 None:intergenic
TTCTTTGCTCCCACACGTTC+TGG 0.281144 7:+101002240 None:intergenic
GGCTTCACCCTCTAAGTTAA+AGG 0.292607 7:-100994044 MsaT040510.1:CDS
TCAATTAGATTTAGAGAATC+AGG 0.292631 7:-101002426 MsaT040510.1:CDS
GTTGCCATGCAAATGGGGTT+GGG 0.298299 7:-100994301 MsaT040510.1:CDS
CAGGGTTGATTTGAAACCTT+TGG 0.301441 7:-101002106 MsaT040510.1:CDS
AGACCAAATCCTCAATTGTT+TGG 0.305048 7:-100994205 MsaT040510.1:CDS
CACTATTTACATCGTAGTTC+TGG 0.309710 7:-101002681 MsaT040510.1:CDS
AAGGGTCATGATAAATATTC+AGG 0.318516 7:-101002855 MsaT040510.1:CDS
CGATAGTGCCCATGCTTTGC+TGG 0.321114 7:-100993478 MsaT040510.1:CDS
AGATTTAGAGAATCAGGTTT+AGG 0.342984 7:-101002420 MsaT040510.1:CDS
GACCAAATCCTCAATTGTTT+GGG 0.348880 7:-100994204 MsaT040510.1:CDS
GGTTTCTTACATGAACTATC+TGG 0.353172 7:-101002660 MsaT040510.1:CDS
GTTTGGGCTTCAAGCTGGTC+AGG 0.356213 7:-100994188 MsaT040510.1:CDS
CATTGGATCCTTTAACTTAG+AGG 0.361856 7:+100994036 None:intergenic
ATTGGAAGTTTGATCAACTT+TGG 0.364253 7:+101002023 None:intergenic
TTGCCATGCAAATGGGGTTG+GGG 0.365073 7:-100994300 MsaT040510.1:CDS
TCCCCTGTTTGCTCTGACAA+TGG 0.365155 7:-101001928 MsaT040510.1:CDS
GAGTCCATCTCATACTCTTA+AGG 0.368152 7:-101002874 MsaT040510.1:CDS
CAACGGCATATTTAACTGAT+TGG 0.373942 7:+101002476 None:intergenic
GATCATGGACGAGGCGTTAT+GGG 0.378376 7:-101001685 MsaT040510.1:CDS
ACGATTTGTTTCAGTTGGTT+TGG 0.382384 7:-101002953 MsaT040510.1:CDS
TCTGGACTTGCTGCTTTACA+TGG 0.382675 7:-100994235 MsaT040510.1:CDS
TGATCATGGACGAGGCGTTA+TGG 0.385981 7:-101001686 MsaT040510.1:CDS
TGTTGCCATGCAAATGGGGT+TGG 0.393996 7:-100994302 MsaT040510.1:CDS
GGCAACACTCATGGGTGAAA+AGG 0.394110 7:+100994318 None:intergenic
TTCATTACGGTTGAGGAAGA+TGG 0.397344 7:+101002336 None:intergenic
ATTCCTGCTGGTAACGTCAT+AGG 0.398850 7:+100994370 None:intergenic
TCATCTTGACTTGAAGAAAC+AGG 0.406226 7:+101002753 None:intergenic
TCATCAAAAGTTGATTTAAG+TGG 0.412874 7:-101002180 MsaT040510.1:CDS
ACTCTCACCAAGAACTTGAT+TGG 0.416264 7:+101002005 None:intergenic
ATCAGTCAGTGGAGCCATCC+AGG 0.417657 7:-101002125 MsaT040510.1:CDS
AGGTCAACCTTGTTGCAATT+CGG 0.419974 7:+101001427 None:intergenic
AAAATGGGTGAAATTCATTA+CGG 0.424400 7:+101002323 None:intergenic
ACAGCTGATGAGTTCATAAC+AGG 0.425607 7:+100994115 None:intergenic
GCGCCATTGTCAGAGCAAAC+AGG 0.425798 7:+101001925 None:intergenic
AACTGAAACAAATCGTTTCT+GGG 0.426922 7:+101002960 None:intergenic
CTTCCTGTTGCACATACATC+TGG 0.427695 7:-100994253 MsaT040510.1:CDS
AGTCCAGATGTATGTGCAAC+AGG 0.429077 7:+100994250 None:intergenic
CAATTGTTTGGGCTTCAAGC+TGG 0.432706 7:-100994193 MsaT040510.1:CDS
ATTGACCATGCACTAAAATC+TGG 0.439314 7:+101002922 None:intergenic
CATTTGCATGGCAACACTCA+TGG 0.440440 7:+100994309 None:intergenic
ACTTACACAGATGATTGATC+TGG 0.443296 7:+101003091 None:intergenic
AAGTTTGAGGATTTAGATAA+CGG 0.451329 7:-101002798 MsaT040510.1:CDS
AAGCAAAGAAAATTTATTTG+TGG 0.456578 7:+101002614 None:intergenic
CATGAGTGTTGCCATGCAAA+TGG 0.458192 7:-100994308 MsaT040510.1:CDS
AACAAAGATGCAGCTGTGAT+AGG 0.462201 7:-101002219 MsaT040510.1:CDS
ACTAATGGAAACCGTGGAAT+CGG 0.464726 7:-100994065 MsaT040510.1:CDS
AGTCCATCTCATACTCTTAA+GGG 0.467462 7:-101002873 MsaT040510.1:CDS
AAATCTAATTGATTCAAATG+AGG 0.470228 7:+101002437 None:intergenic
AATTGCAAGTGAAGAACCCT+TGG 0.474008 7:+101002533 None:intergenic
ATGAGTGTTGCCATGCAAAT+GGG 0.476275 7:-100994307 MsaT040510.1:CDS
CAGAAACGATTTGTTTCAGT+TGG 0.477357 7:-101002958 MsaT040510.1:CDS
TGCCATGCAAATGGGGTTGG+GGG 0.478491 7:-100994299 MsaT040510.1:CDS
GCAACACTCATGGGTGAAAA+GGG 0.478775 7:+100994319 None:intergenic
CTTGGTGAGAGTCGCAGAAA+AGG 0.480139 7:-101001994 MsaT040510.1:CDS
GATACATCTTCTTTGCAAGC+AGG 0.485143 7:+101002045 None:intergenic
GGTGAAATTCATTACGGTTG+AGG 0.487024 7:+101002329 None:intergenic
TGAGTTCATAACAGGCTCTC+CGG 0.490845 7:+100994123 None:intergenic
AATCTAATTGATTCAAATGA+GGG 0.495878 7:+101002438 None:intergenic
TTTGGGCTTCAAGCTGGTCA+GGG 0.497255 7:-100994187 MsaT040510.1:CDS
AGGTGAGTTCCTTGAAATTG+AGG 0.498352 7:+100994274 None:intergenic
TACAAGGATAGGAAAGTTTG+AGG 0.498732 7:-101002811 MsaT040510.1:CDS
GCTTGAAGCCCAAACAATTG+AGG 0.502101 7:+100994196 None:intergenic
CACTGACTGATGACAATGTA+TGG 0.502818 7:+101002137 None:intergenic
AACAAATCAAATGTCTATCC+AGG 0.503101 7:-100993966 MsaT040510.1:intron
AAAGCTAATCTTCAGAACAT+TGG 0.503501 7:-101002279 MsaT040510.1:CDS
TGCATGTGCTGCATTCCTGC+TGG 0.506406 7:+100994358 None:intergenic
GAGTTCATAACAGGCTCTCC+GGG 0.506487 7:+100994124 None:intergenic
TAGTGGAACAGAAGGACTAA+TGG 0.508403 7:-100994080 MsaT040510.1:CDS
TTTCTTCACAATGTGCATGC+TGG 0.509695 7:+101002370 None:intergenic
ATTGGATCCTTTAACTTAGA+GGG 0.511226 7:+100994037 None:intergenic
AATGAAACAAATACAAGGAT+AGG 0.513186 7:-101002822 MsaT040510.1:CDS
TTAGGTGAGAATAATACAAA+AGG 0.513763 7:-101002402 MsaT040510.1:CDS
GGTGTTAAAAGAACTGATCA+TGG 0.513840 7:-101001700 MsaT040510.1:CDS
CGCCATTGTCAGAGCAAACA+GGG 0.514057 7:+101001926 None:intergenic
TAAACAAGGTATCCTTTCCA+AGG 0.516786 7:-101002550 MsaT040510.1:CDS
AACATTGAAGCACATGGCAT+TGG 0.518946 7:+100994154 None:intergenic
GGCGGATAAAGCTTCAATGC+TGG 0.519274 7:-100994580 MsaT040510.1:CDS
TTCAGATAGATTGTGCACTT+CGG 0.519860 7:+101001642 None:intergenic
AGGAATGCAGCACATGCATG+CGG 0.519863 7:-100994353 MsaT040510.1:CDS
TCATTACGGTTGAGGAAGAT+GGG 0.521159 7:+101002337 None:intergenic
CTTACACAGATGATTGATCT+GGG 0.521769 7:+101003092 None:intergenic
TCAAGAGATTTCGGCTCCAA+AGG 0.523726 7:+101002090 None:intergenic
TTTACGCAGATTATGACAAT+GGG 0.530246 7:-100994412 MsaT040510.1:intron
ATTTGATGACGATGATGCCT+TGG 0.533845 7:-101002728 MsaT040510.1:CDS
TAAAGCTTCAATGCTGGATG+AGG 0.536704 7:-100994574 MsaT040510.1:CDS
GTTTGAATGAAATTATTACA+AGG 0.536877 7:-101002776 MsaT040510.1:CDS
GCTGTAGAATTAAATGCTAG+TGG 0.540713 7:-100994097 MsaT040510.1:CDS
TGGTAGTGTGAAGCAACAAC+CGG 0.544994 7:-100993528 MsaT040510.1:intron
ATTATGACAATGGGAAGTCA+TGG 0.554557 7:-100994403 MsaT040510.1:CDS
TCATGCAGAGACGAAGAGAC+AGG 0.554875 7:-101001486 MsaT040510.1:intron
TGTGAGTTCACATTTGACAT+TGG 0.556678 7:+100994019 None:intergenic
AGAGAAAGAATACTTGCAGT+TGG 0.557431 7:+100994523 None:intergenic
AGTGCACAATCTATCTGAAA+GGG 0.562116 7:-101001638 MsaT040510.1:intron
ATTTGCATGGCAACACTCAT+GGG 0.564314 7:+100994310 None:intergenic
TTAAATGCTAGTGGAACAGA+AGG 0.566465 7:-100994088 MsaT040510.1:CDS
AAGTGCACAATCTATCTGAA+AGG 0.566770 7:-101001639 MsaT040510.1:CDS
TTCATCAAGACCAGAACGTG+TGG 0.573082 7:-101002250 MsaT040510.1:CDS
ACTTGCTGCTTTACATGGGA+TGG 0.575642 7:-100994230 MsaT040510.1:CDS
CTGGACTTGCTGCTTTACAT+GGG 0.587526 7:-100994234 MsaT040510.1:CDS
ATGCCTATGACGTTACCAGC+AGG 0.590867 7:-100994373 MsaT040510.1:CDS
GGTTTCAAATCAACCCTGGA+TGG 0.593516 7:+101002111 None:intergenic
AGAGGGTGAAGCCGATTCCA+CGG 0.595574 7:+100994054 None:intergenic
TTAGCTTTCACAATAGCCGC+AGG 0.598322 7:+101002294 None:intergenic
GCCTTGGTTGAATTGTGCAA+TGG 0.600384 7:-101002712 MsaT040510.1:CDS
GGCAGCAAAGTCATTTGTAG+TGG 0.603034 7:+101002635 None:intergenic
CAAAGGTTTCAAATCAACCC+TGG 0.605010 7:+101002107 None:intergenic
AAACAAGGTATCCTTTCCAA+GGG 0.605300 7:-101002549 MsaT040510.1:CDS
TGGACTTGAACAATCAAAAG+TGG 0.608631 7:-100993505 MsaT040510.1:CDS
TCAGTCAGTGGAGCCATCCA+GGG 0.609277 7:-101002124 MsaT040510.1:CDS
CAGATGTATGTGCAACAGGA+AGG 0.625034 7:+100994254 None:intergenic
AAAAGAACTGATCATGGACG+AGG 0.626096 7:-101001694 MsaT040510.1:CDS
TGACCCTTAAGAGTATGAGA+TGG 0.635956 7:+101002870 None:intergenic
CAAGTGAAGAACCCTTGGAA+AGG 0.639273 7:+101002538 None:intergenic
TGAGTGTTGCCATGCAAATG+GGG 0.642524 7:-100994306 MsaT040510.1:CDS
ATGCTAATGAAACAAATACA+AGG 0.649445 7:-101002827 MsaT040510.1:CDS
TCATCAAGACCAGAACGTGT+GGG 0.654328 7:-101002249 MsaT040510.1:CDS
TCCATTGCACAATTCAACCA+AGG 0.657257 7:+101002711 None:intergenic
ATGTGGCATAACTCATGCAG+AGG 0.662299 7:+101003160 None:intergenic
AGAAGGACTAATGGAAACCG+TGG 0.662312 7:-100994071 MsaT040510.1:CDS
GAAGAGACAGGATCAATGAG+AGG 0.663392 7:-101001474 MsaT040510.1:CDS
TAAGAAAACATTGAAGCACA+TGG 0.666915 7:+100994148 None:intergenic
GGTTGACATTGATGCAACAA+CGG 0.669249 7:+101002459 None:intergenic
ACTCATACCGAATTGCAACA+AGG 0.682291 7:-101001434 MsaT040510.1:intron
TTTAGTGACTCTCATAAACA+AGG 0.682360 7:-101002564 MsaT040510.1:CDS
TAGTGTGAAGCAACAACCGG+TGG 0.694277 7:-100993525 MsaT040510.1:CDS
CATACATTGTCATCAGTCAG+TGG 0.712438 7:-101002136 MsaT040510.1:CDS
TTACACAGATGATTGATCTG+GGG 0.713496 7:+101003093 None:intergenic
GCCATTGTCAGAGCAAACAG+GGG 0.723809 7:+101001927 None:intergenic
TGGACTATGAGGATTCTGAG+TGG 0.766905 7:-101001855 MsaT040510.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 100993431 101003188 100993431 ID=MsaG040510
Chr7 mRNA 100993431 101003188 100993431 ID=MsaT040510.1;Parent=MsaG040510
Chr7 exon 100993431 100993544 100993431 ID=MsaT040510.1.exon7;Parent=MsaT040510.1
Chr7 CDS 100993431 100993544 100993431 ID=cds.MsaT040510.1;Parent=MsaT040510.1
Chr7 exon 100993967 100994425 100993967 ID=MsaT040510.1.exon6;Parent=MsaT040510.1
Chr7 CDS 100993967 100994425 100993967 ID=cds.MsaT040510.1;Parent=MsaT040510.1
Chr7 exon 100994536 100994601 100994536 ID=MsaT040510.1.exon5;Parent=MsaT040510.1
Chr7 CDS 100994536 100994601 100994536 ID=cds.MsaT040510.1;Parent=MsaT040510.1
Chr7 exon 101001435 101001500 101001435 ID=MsaT040510.1.exon4;Parent=MsaT040510.1
Chr7 CDS 101001435 101001500 101001435 ID=cds.MsaT040510.1;Parent=MsaT040510.1
Chr7 exon 101001639 101001743 101001639 ID=MsaT040510.1.exon3;Parent=MsaT040510.1
Chr7 CDS 101001639 101001743 101001639 ID=cds.MsaT040510.1;Parent=MsaT040510.1
Chr7 exon 101001846 101002983 101001846 ID=MsaT040510.1.exon2;Parent=MsaT040510.1
Chr7 CDS 101001846 101002983 101001846 ID=cds.MsaT040510.1;Parent=MsaT040510.1
Chr7 exon 101003097 101003188 101003097 ID=MsaT040510.1.exon1;Parent=MsaT040510.1
Chr7 CDS 101003097 101003188 101003097 ID=cds.MsaT040510.1;Parent=MsaT040510.1
Gene Sequence

>MsaT040510.1

ATGATGCCTCTGCATGAGTTATGCCACATGTCTAAAGAAAAAGTTGATCATAAAGAGATGAACAATTCTTGTACCCCAGATCAATCATCTGTACCCAGAAACGATTTGTTTCAGTTGGTTTGGCAAAATGACCAGATTTTAGTGCATGGTCAATCTAGTAATTCCAATAGAGCTAAAACGAGTCCATCTCATACTCTTAAGGGTCATGATAAATATTCAGGACAACATGCTAATGAAACAAATACAAGGATAGGAAAGTTTGAGGATTTAGATAACGGTTTGAATGAAATTATTACAAGGCCTGTTTCTTCAAGTCAAGATGAAGATTTGATGACGATGATGCCTTGGTTGAATTGTGCAATGGATGATGAACACTATTTACATCGTAGTTCTGGTTTCTTACATGAACTATCTGGTGCCACTACAAATGACTTTGCTGCCACAAATAAATTTTCTTTGCTTGATAGAAAAAGTAATTGTATTCAAGTATTTAGTGACTCTCATAAACAAGGTATCCTTTCCAAGGGTTCTTCACTTGCAATTGAAGATTTTGATACCTCGGGACTAAAAACTAGCACCAATCAGTTAAATATGCCGTTGTTGCATCAATGTCAACCCTCATTTGAATCAATTAGATTTAGAGAATCAGGTTTAGGTGAGAATAATACAAAAGGTAATGCAAACCAGCATGCACATTGTGAAGAAATCTCTCATATCCCATCTTCCTCAACCGTAATGAATTTCACCCATTTTGCAAAACCTGCGGCTATTGTGAAAGCTAATCTTCAGAACATTGGTTTCTCTTCATCAAGACCAGAACGTGTGGGAGCAAAGAACAAAGATGCAGCTGTGATAGGAAGTAATCCTTGCGAATCATCAAAAGTTGATTTAAGTGGAGAATGTCCAAAAAGTTCAGCCATACATTGTCATCAGTCAGTGGAGCCATCCAGGGTTGATTTGAAACCTTTGGAGCCGAAATCTCTTGAGAAAAACACTAGTGTTTCTACTCCTGCTTGCAAAGAAGATGTATCCAAAGTTGATCAAACTTCCAATCAAGTTCTTGGTGAGAGTCGCAGAAAAGGTCAAGAATCTTTTAAAAAATCTACGGAGCTAACAGTAGTCTCTTCCCCTGTTTGCTCTGACAATGGCGCGTATAGAAGTTCAGATGATGCAAATCAAAATTTGAAGCGAAAAAATCTGGACTATGAGGATTCTGAGTGGCACAGTGAAGATTTTGAAGATGAATCAATTGGTGTTAAAAGAACTGATCATGGACGAGGCGTTATGGGCTCAAAGAAAAATCGTTCAGCCGAAGTGCACAATCTATCTGAAAGGAGACGAAGAGACAGGATCAATGAGAGGATGCGTGCATTGCAAGAACTCATACCGAATTGCAACAAGGCGGATAAAGCTTCAATGCTGGATGAGGCAATTGAGTATCTTAAATCACTTCAACTCCAACTGCAAATTATGACAATGGGAAGTCATGGTTTATACATGCCTATGACGTTACCAGCAGGAATGCAGCACATGCATGCGGCTCACATGTTTCCCTTTTCACCCATGAGTGTTGCCATGCAAATGGGGTTGGGGGTGCCTCAATTTCAAGGAACTCACCTTCCTGTTGCACATACATCTGGACTTGCTGCTTTACATGGGATGGCAAGACCAAATCCTCAATTGTTTGGGCTTCAAGCTGGTCAGGGACTGCACATGCCAATGCCATGTGCTTCAATGTTTTCTTATCCCGGAGAGCCTGTTATGAACTCATCAGCTGTAGAATTAAATGCTAGTGGAACAGAAGGACTAATGGAAACCGTGGAATCGGCTTCACCCTCTAAGTTAAAGGATCCAATGTCAAATGTGAACTCACAAGTTATGCTTAAAAACAAGGGATGTAACTCAACAAATCAAATGTCTATCCAGTGTGAAGCAACAACCGGTGGACTTGAACAATCAAAAGTGGTACTCGATAGTGCCCATGCTTTGCTGGAAAATGACAAAAGAGACAACGTTGAGATTGATAAATCTCATGAGTAA
Protein sequence

>MsaT040510.1

MMPLHELCHMSKEKVDHKEMNNSCTPDQSSVPRNDLFQLVWQNDQILVHGQSSNSNRAKTSPSHTLKGHDKYSGQHANETNTRIGKFEDLDNGLNEIITRPVSSSQDEDLMTMMPWLNCAMDDEHYLHRSSGFLHELSGATTNDFAATNKFSLLDRKSNCIQVFSDSHKQGILSKGSSLAIEDFDTSGLKTSTNQLNMPLLHQCQPSFESIRFRESGLGENNTKGNANQHAHCEEISHIPSSSTVMNFTHFAKPAAIVKANLQNIGFSSSRPERVGAKNKDAAVIGSNPCESSKVDLSGECPKSSAIHCHQSVEPSRVDLKPLEPKSLEKNTSVSTPACKEDVSKVDQTSNQVLGESRRKGQESFKKSTELTVVSSPVCSDNGAYRSSDDANQNLKRKNLDYEDSEWHSEDFEDESIGVKRTDHGRGVMGSKKNRSAEVHNLSERRRRDRINERMRALQELIPNCNKADKASMLDEAIEYLKSLQLQLQIMTMGSHGLYMPMTLPAGMQHMHAAHMFPFSPMSVAMQMGLGVPQFQGTHLPVAHTSGLAALHGMARPNPQLFGLQAGQGLHMPMPCASMFSYPGEPVMNSSAVELNASGTEGLMETVESASPSKLKDPMSNVNSQVMLKNKGCNSTNQMSIQCEATTGGLEQSKVVLDSAHALLENDKRDNVEIDKSHE*