AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040703


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040703 MtrunA17_Chr7g0273661 97.436 156 4 0 4 159 1 156 2.14e-112 317
MsaG040703 MtrunA17_Chr7g0223361 58.523 176 72 1 4 179 1 175 2.65e-71 213
MsaG040703 MtrunA17_Chr7g0223351 60.920 174 66 2 4 177 1 172 3.78e-67 203
MsaG040703 MtrunA17_Chr1g0204971 46.784 171 86 2 7 174 19 187 5.30e-48 155
MsaG040703 MtrunA17_Chr1g0204951 45.614 171 88 2 7 174 19 187 5.67e-47 152
MsaG040703 MtrunA17_Chr5g0394621 42.442 172 96 1 6 174 16 187 1.12e-46 151
MsaG040703 MtrunA17_Chr5g0394631 42.604 169 94 1 3 168 13 181 2.21e-43 149
MsaG040703 MtrunA17_Chr5g0394631 43.429 175 90 5 7 174 226 398 2.54e-39 138
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040703 AT3G22560.1 55.422 166 72 1 11 174 7 172 1.06e-63 194
MsaG040703 AT2G32030.1 47.305 167 84 2 11 176 24 187 4.79e-57 178
MsaG040703 AT2G32020.1 47.701 174 87 2 4 176 12 182 1.21e-56 177

Find 44 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTGTTGCTTCTAACTATT+GGG 0.152318 7:+102991105 MsaT040703.1:CDS
TTCTGATTTGTTAAGGTTGC+AGG 0.201561 7:+102991186 MsaT040703.1:CDS
ATGCTGTTGCTTCTAACTAT+TGG 0.211993 7:+102991104 MsaT040703.1:CDS
CAAGGCTTCTCAAAGAGTTT+TGG 0.245031 7:+102991228 MsaT040703.1:CDS
AGACATCTTTGATGAAAGTT+AGG 0.305845 7:-102990973 None:intergenic
CAAAGGTACCATCATGGATT+TGG 0.335385 7:+102991300 MsaT040703.1:CDS
AATAGATCATAAAGAGTAAC+AGG 0.344936 7:-102991377 None:intergenic
CGTTGACGATTTCATGTTAT+GGG 0.361603 7:+102990889 MsaT040703.1:CDS
TTCTAAGAACACCCTCTCTA+AGG 0.383141 7:-102991264 None:intergenic
ACGTTGACGATTTCATGTTA+TGG 0.384226 7:+102990888 MsaT040703.1:CDS
TCAACGTCTGTTAGCTTGAA+TGG 0.418956 7:-102990872 None:intergenic
GTTTCTGTGTATCCTTGGTC+AGG 0.429314 7:+102991052 MsaT040703.1:CDS
GACGATTTCATGTTATGGGC+AGG 0.454598 7:+102990893 MsaT040703.1:CDS
GACATCTTTGATGAAAGTTA+GGG 0.457791 7:-102990972 None:intergenic
CAGGAGACAAAATAATCAAC+AGG 0.459432 7:-102991358 None:intergenic
TGGCAAAGGTGGATCTATCT+AGG 0.472926 7:+102990837 MsaT040703.1:CDS
TAGATCCACCTTTGCCATGT+TGG 0.487434 7:-102990831 None:intergenic
CGATTTCATGTTATGGGCAG+GGG 0.502678 7:+102990895 MsaT040703.1:CDS
GCTTCTAACTATTGGGGTCA+AGG 0.506483 7:+102991112 MsaT040703.1:CDS
ATCGAATGACGCCAAGGGTG+AGG 0.527528 7:-102991001 None:intergenic
TGTCTCGACGACCGTTCCAT+CGG 0.528351 7:+102991025 MsaT040703.1:CDS
GCTGTTGCTTCTAACTATTG+GGG 0.550437 7:+102991106 MsaT040703.1:CDS
AGAAAGTATACTTATATCAA+AGG 0.562044 7:+102991283 MsaT040703.1:CDS
CTTCTAACTATTGGGGTCAA+GGG 0.563961 7:+102991113 MsaT040703.1:CDS
ATCCTTGGTCAGGTGATGAT+AGG 0.566062 7:+102991062 MsaT040703.1:CDS
GATGGAAAACTTGCAATTCG+AGG 0.572925 7:+102990942 MsaT040703.1:CDS
TATGACCAACATGGCAAAGG+TGG 0.575392 7:+102990826 None:intergenic
ACGATTTCATGTTATGGGCA+GGG 0.580454 7:+102990894 MsaT040703.1:CDS
GATAGGTGCAAAGCTGACAT+AGG 0.586688 7:+102991079 MsaT040703.1:CDS
ATGGAAAACTTGCAATTCGA+GGG 0.591968 7:+102990943 MsaT040703.1:CDS
ATGTCTGCATACCTCACCCT+TGG 0.593939 7:+102990990 MsaT040703.1:CDS
TTATATCAAAGGTACCATCA+TGG 0.596125 7:+102991294 MsaT040703.1:CDS
AGACAGATCGAATGACGCCA+AGG 0.596639 7:-102991007 None:intergenic
CAATGATCATATGACCAACA+TGG 0.603556 7:+102990817 None:intergenic
TCATATGACCAACATGGCAA+AGG 0.606489 7:+102990823 None:intergenic
AAAGGCTGGGTTCCTTAGAG+AGG 0.612674 7:+102991252 MsaT040703.1:CDS
ATACACAGAAACAAAACCGA+TGG 0.615405 7:-102991041 None:intergenic
AAAGACAGCCAAATCCATGA+TGG 0.623533 7:-102991308 None:intergenic
GACAGATCGAATGACGCCAA+GGG 0.628805 7:-102991006 None:intergenic
CAGAAACAAAACCGATGGAA+CGG 0.641063 7:-102991036 None:intergenic
AAGGCTGGGTTCCTTAGAGA+GGG 0.646930 7:+102991253 MsaT040703.1:CDS
GAGACAAAATAATCAACAGG+AGG 0.670315 7:-102991355 None:intergenic
CACCTATCATCACCTGACCA+AGG 0.677858 7:-102991064 None:intergenic
ATCTATCTAGGATATCTCTG+AGG 0.735761 7:+102990849 MsaT040703.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 102990827 102991393 102990827 ID=MsaG040703
Chr7 mRNA 102990827 102991393 102990827 ID=MsaT040703.1;Parent=MsaG040703
Chr7 exon 102990827 102991393 102990827 ID=MsaT040703.1.exon1;Parent=MsaT040703.1
Chr7 CDS 102990827 102991393 102990827 ID=cds.MsaT040703.1;Parent=MsaT040703.1
Gene Sequence

>MsaT040703.1

ATGACCAACATGGCAAAGGTGGATCTATCTAGGATATCTCTGAGGCCATTCAAGCTAACAGACGTTGACGATTTCATGTTATGGGCAGGGGACGATGAAGTAACCAAAAACATCCGATGGAAAACTTGCAATTCGAGGGAAGAAGCCCTAACTTTCATCAAAGATGTCTGCATACCTCACCCTTGGCGTCATTCGATCTGTCTCGACGACCGTTCCATCGGTTTTGTTTCTGTGTATCCTTGGTCAGGTGATGATAGGTGCAAAGCTGACATAGGTTATGCTGTTGCTTCTAACTATTGGGGTCAAGGGATAGCCACAAAAGCAATCAAAATTGCTCTGTCTCAAGTGTTTAATGATTTTTCTGATTTGTTAAGGTTGCAGGCTTTTACTTACGTGGATAACAAGGCTTCTCAAAGAGTTTTGGAAAAGGCTGGGTTCCTTAGAGAGGGTGTTCTTAGAAAGTATACTTATATCAAAGGTACCATCATGGATTTGGCTGTCTTTAGTTTTTTGTCAACAGATGAAATTCCTCCTGTTGATTATTTTGTCTCCTGTTACTCTTTATGA

Protein sequence

>MsaT040703.1

MTNMAKVDLSRISLRPFKLTDVDDFMLWAGDDEVTKNIRWKTCNSREEALTFIKDVCIPHPWRHSICLDDRSIGFVSVYPWSGDDRCKADIGYAVASNYWGQGIATKAIKIALSQVFNDFSDLLRLQAFTYVDNKASQRVLEKAGFLREGVLRKYTYIKGTIMDLAVFSFLSTDEIPPVDYFVSCYSL*