AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040722

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040722 MtrunA17_Chr7g0273921 98.276 348 6 0 1 348 59 406 0.0 709
MsaG040722 MtrunA17_Chr7g0273941 87.940 398 22 5 663 1058 110 483 0.0 652
MsaG040722 MtrunA17_Chr7g0273931 98.479 263 4 0 399 661 1 263 0.0 532
MsaG040722 MtrunA17_Chr1g0190851 46.789 436 173 15 667 1063 98 513 2.02e-97 320
MsaG040722 MtrunA17_Chr2g0290511 31.250 256 137 9 816 1052 114 349 2.75e-29 121
MsaG040722 MtrunA17_Chr6g0463941 55.238 105 38 2 956 1060 464 559 1.83e-27 119
MsaG040722 MtrunA17_Chr6g0463941 41.837 98 47 2 956 1053 55 142 1.67e-14 78.2
MsaG040722 MtrunA17_Chr1g0201941 44.792 96 43 2 956 1051 57 142 3.09e-15 80.5
MsaG040722 MtrunA17_Chr1g0201911 42.424 99 47 2 956 1054 55 143 1.71e-14 77.8
MsaG040722 MtrunA17_Chr1g0198171 42.268 97 45 3 956 1051 114 200 3.35e-13 73.6
MsaG040722 MtrunA17_Chr7g0265831 39.000 100 50 3 956 1054 115 204 4.93e-13 72.8
MsaG040722 MtrunA17_Chr1g0179191 38.462 104 53 3 956 1058 131 224 1.57e-12 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040722 AT3G04820.1 59.195 696 226 7 1 662 37 708 0.0 821
MsaG040722 AT5G28300.1 47.143 280 93 7 813 1054 296 558 2.63e-63 227
MsaG040722 AT1G76890.2 37.818 275 131 10 791 1055 243 487 5.85e-39 154
MsaG040722 AT1G76890.2 36.111 144 76 4 956 1093 41 174 5.21e-15 80.1
MsaG040722 AT1G76880.1 54.000 100 37 2 956 1055 408 498 5.89e-26 114
MsaG040722 AT1G76880.1 38.760 129 60 4 947 1066 48 166 1.23e-15 82.0
MsaG040722 AT5G47660.1 30.364 247 144 7 807 1052 169 388 3.07e-25 110
MsaG040722 AT1G33240.3 56.701 97 33 3 956 1052 435 522 5.06e-25 112
MsaG040722 AT1G33240.3 42.708 96 45 2 956 1051 62 147 3.82e-16 83.6
MsaG040722 AT1G33240.2 56.701 97 33 3 956 1052 396 483 6.34e-25 111
MsaG040722 AT1G33240.1 56.701 97 33 3 956 1052 435 522 6.82e-25 112
MsaG040722 AT1G33240.1 42.708 96 45 2 956 1051 62 147 3.59e-16 84.0
MsaG040722 AT3G10000.1 40.000 100 50 3 956 1054 88 178 1.31e-13 75.1
MsaG040722 AT5G03680.1 38.776 98 49 3 956 1052 119 206 1.35e-12 72.4
MsaG040722 AT3G10000.2 36.364 99 45 3 956 1054 88 168 5.08e-11 67.0

Find 216 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTATTCCACGAACTTTA+AGG 0.099204 7:+103249367 MsaT040722.1:CDS
GTATTAAAGTGGGTGATTTC+TGG 0.173309 7:+103248220 MsaT040722.1:CDS
AGATTGTTACAAGGATTATT+AGG 0.181963 7:-103261053 None:intergenic
TACCAAACAACTTCTCATTA+AGG 0.205495 7:-103248200 None:intergenic
GAGACAGATTTAGATAAAAT+TGG 0.218605 7:+103256896 MsaT040722.1:CDS
CGCACTGTGTTGACAATTAT+AGG 0.247542 7:-103256929 None:intergenic
ACAGTAGAGGTTCTGAAAAT+TGG 0.256567 7:+103246408 MsaT040722.1:CDS
TATCTTTGTTCTCCTTATAA+AGG 0.275273 7:-103247246 None:intergenic
TGATCAACGGTGGTGGAGTT+TGG 0.283034 7:-103260243 None:intergenic
TCTAATTTCTCCATTATTGT+AGG 0.283920 7:-103261170 None:intergenic
CCTTGTTGTGCCTACAATAA+TGG 0.294174 7:+103261160 MsaT040722.1:CDS
TGTGGTAATATTACTTCCTT+TGG 0.298357 7:-103257019 None:intergenic
TGCTACATTTCCAACCTTCC+TGG 0.299243 7:+103245240 MsaT040722.1:CDS
TTCTCGATCACCAGTGTTAC+TGG 0.306160 7:+103256682 MsaT040722.1:CDS
TGTTGAAGGAAATAGTAATA+AGG 0.308788 7:+103261280 MsaT040722.1:CDS
TTATAGGTTTCACCTTTATA+AGG 0.313323 7:+103247234 MsaT040722.1:intron
CAAAGATACCCAGGAAGCTT+TGG 0.321227 7:+103247261 MsaT040722.1:CDS
TAGGGAAAACTTCAAGTTCC+TGG 0.321615 7:+103246259 MsaT040722.1:CDS
CCGTTTCAGCAACTAAGAAT+TGG 0.321676 7:-103249142 None:intergenic
GAACTGAGTTGATGAAAATA+AGG 0.325543 7:-103261464 None:intergenic
CGTTTCAGCAACTAAGAATT+GGG 0.329151 7:-103249141 None:intergenic
CTCGGTCACCATGAATTCTA+TGG 0.329174 7:+103245194 None:intergenic
TACAATATGACCATTTGAAT+TGG 0.335962 7:-103261555 None:intergenic
GGATTCTGATGCGTCTAGTT+TGG 0.338991 7:+103245690 MsaT040722.1:CDS
GTGTTCTTCCATAGAATTCA+TGG 0.340177 7:-103245202 None:intergenic
GGAACTGAACAAGTAGTATT+AGG 0.341715 7:+103250436 MsaT040722.1:intron
CATCATCAGAGCTCTCTAAT+TGG 0.343057 7:-103256994 None:intergenic
GATTGTTACAAGGATTATTA+GGG 0.345761 7:-103261052 None:intergenic
TCTGCTATCACATTAGAAAA+AGG 0.348185 7:-103261089 None:intergenic
ATGTTCACTGCTCAGCGTTT+TGG 0.349350 7:+103248817 MsaT040722.1:intron
AACTGACAGTCGAGATCATT+TGG 0.352346 7:+103247972 MsaT040722.1:intron
AAAGCCTGCAAGTAGTTTCC+AGG 0.358570 7:-103249342 None:intergenic
AGAAACCAATTGACTTTGAA+TGG 0.360331 7:+103256725 MsaT040722.1:CDS
AAAGATACCCAGGAAGCTTT+GGG 0.362115 7:+103247262 MsaT040722.1:CDS
ATAACTTATAACTTCAGTTT+CGG 0.362929 7:-103245632 None:intergenic
TGCAGTAAGCTAGCTGAGTT+TGG 0.364718 7:+103260447 MsaT040722.1:intron
CCGCAGTCATCATGACATTC+AGG 0.367694 7:-103250514 None:intergenic
TCCCTTAATGAGAAGTTGTT+TGG 0.368656 7:+103248198 MsaT040722.1:CDS
TCGAGATCATTTGGATTTGC+GGG 0.369117 7:+103247981 MsaT040722.1:CDS
CTCTGTCTTGGTCAGCTATT+CGG 0.371127 7:+103248344 MsaT040722.1:CDS
CGAATCATCTTCTTCGTGTT+GGG 0.376149 7:-103260655 None:intergenic
GAATCCTTTAAGTCTCTTGC+GGG 0.385870 7:+103245667 MsaT040722.1:CDS
AAAGATACCAATGATGTTGC+TGG 0.386798 7:+103249101 MsaT040722.1:CDS
ATACACTTTGACAATATCAT+TGG 0.387320 7:-103255197 None:intergenic
GTTGGATGTTTGGTTAATTG+AGG 0.388574 7:-103261530 None:intergenic
TGAATTGGAGGTTGGATGTT+TGG 0.388613 7:-103261540 None:intergenic
GATCAACGGTGGTGGAGTTT+GGG 0.391573 7:-103260242 None:intergenic
GTACATGAAGTAGATTTAGA+TGG 0.399108 7:+103245385 MsaT040722.1:CDS
GTTGAAGGAAATAGTAATAA+GGG 0.400371 7:+103261281 MsaT040722.1:CDS
CATGGCTTCATTAACTACTT+TGG 0.402779 7:+103248588 MsaT040722.1:CDS
ACCAAACAACTTCTCATTAA+GGG 0.404620 7:-103248199 None:intergenic
TACAATAATGGAGAAATTAG+AGG 0.413562 7:+103261172 MsaT040722.1:CDS
CAGTCTTCTGATTCTCCAAT+GGG 0.423197 7:+103261596 MsaT040722.1:CDS
AAATCCTGGAAACTACTTGC+AGG 0.424190 7:+103249338 MsaT040722.1:CDS
CTCTCCGATTACTCTTTCTC+CGG 0.424800 7:+103245756 MsaT040722.1:CDS
GTCGTCATCATCACAGTGTT+TGG 0.428860 7:-103260697 None:intergenic
GTCGAGATCATTTGGATTTG+CGG 0.432531 7:+103247980 MsaT040722.1:intron
TCGAATCATCTTCTTCGTGT+TGG 0.432686 7:-103260656 None:intergenic
CCAGTGTTACTGGAAGTTAC+AGG 0.435321 7:+103256692 MsaT040722.1:CDS
AGGTCAACTTGAGAGCAGTA+TGG 0.438734 7:-103257078 None:intergenic
TCCCATGTGATGAGTTGATC+TGG 0.439124 7:-103260337 None:intergenic
CACATCATCCTTAAAGTTCG+TGG 0.439826 7:-103249375 None:intergenic
CCAATTCTTAGTTGCTGAAA+CGG 0.442115 7:+103249142 MsaT040722.1:CDS
TCAACATTTCAAATCTATCA+AGG 0.442340 7:-103260764 None:intergenic
CTTGAAGATCACTAACAAAC+CGG 0.444929 7:-103260566 None:intergenic
GTTCAAGAATTCAATGATGT+TGG 0.446172 7:-103260976 None:intergenic
CATTGGAGCCGCATTACTCC+GGG 0.446329 7:+103248864 MsaT040722.1:CDS
AAGTTCGTCGTTAGTCCAGC+TGG 0.453547 7:-103260272 None:intergenic
TTGTCTAGTTATGTTAATGA+AGG 0.454954 7:+103248320 MsaT040722.1:intron
GCATTGGAGCCGCATTACTC+CGG 0.456062 7:+103248863 MsaT040722.1:CDS
GAATCCGGAGAAAGAGTAAT+CGG 0.456760 7:-103245760 None:intergenic
AAAATGCTGGCAAGTTCCTT+AGG 0.459083 7:+103246435 MsaT040722.1:CDS
CTCTGAAGGAATGTTTGAGT+TGG 0.461517 7:+103261322 MsaT040722.1:CDS
TCAGAACCTCTACTGTCAAA+AGG 0.461761 7:-103246401 None:intergenic
AGAAGCAACAATAATGTTGA+AGG 0.463341 7:+103261266 MsaT040722.1:CDS
TACTCTGTGTCACTTGAGCT+AGG 0.463574 7:-103261119 None:intergenic
TCTGAAGGAATGTTTGAGTT+GGG 0.464616 7:+103261323 MsaT040722.1:CDS
TTTATTAATCAAATTAGTGC+TGG 0.466579 7:+103245718 MsaT040722.1:CDS
ACAGATTTACAATCACATTA+CGG 0.467355 7:+103248369 MsaT040722.1:CDS
GGGGTGCTTGGACATATGCT+TGG 0.467796 7:+103247283 MsaT040722.1:CDS
AATATGACCATTTGAATTGG+AGG 0.468145 7:-103261552 None:intergenic
AGAAAAGGTCAATGGATTCA+AGG 0.476864 7:+103261435 MsaT040722.1:CDS
TAGTAATAAGGGACCTTTGT+GGG 0.477140 7:+103261292 MsaT040722.1:CDS
ATGTACCATTCAAAGTCAAT+TGG 0.478182 7:-103256730 None:intergenic
GATCTTCAAGATCTTTATCA+AGG 0.479140 7:+103260579 MsaT040722.1:CDS
ACCTCTACTGTCAAAAGGCT+TGG 0.484633 7:-103246396 None:intergenic
CGATGGGATTTATCAGAATC+CGG 0.485429 7:-103245775 None:intergenic
GAAGAGTGCTCAAAATTGTA+AGG 0.485968 7:+103260476 MsaT040722.1:CDS
AAAGCTGCTGCAGATTCCTT+AGG 0.490229 7:+103248561 MsaT040722.1:CDS
CATCCAACCTCCAATTCAAA+TGG 0.490841 7:+103261545 MsaT040722.1:CDS
AAAAGTTTAAAACAAAATCC+TGG 0.496973 7:+103249324 MsaT040722.1:CDS
GCGTGCACTAAATGAGCAAA+AGG 0.498418 7:+103256949 MsaT040722.1:CDS
TCAAGAAAAGCTTGCTAGAG+AGG 0.499159 7:+103260875 MsaT040722.1:CDS
TAATGGAGAAATTAGAGGAT+AGG 0.500977 7:+103261177 MsaT040722.1:CDS
AGTGCCAACACTTCATCTCT+TGG 0.501001 7:-103261209 None:intergenic
AGAAATTAGAGGATAGGAGA+AGG 0.501494 7:+103261183 MsaT040722.1:CDS
GGAATCTAAGCTTGCAGCAG+TGG 0.504509 7:+103245447 MsaT040722.1:CDS
GTTAATGAAGGACTCTGTCT+TGG 0.505352 7:+103248332 MsaT040722.1:CDS
AATAACGAAGATGAAGTCTC+TGG 0.506425 7:+103250553 MsaT040722.1:CDS
GGAACAACGCAGCAAGTACA+AGG 0.508464 7:+103250212 MsaT040722.1:CDS
ACTTTGACAATATCATTGGT+TGG 0.509849 7:-103255193 None:intergenic
TGACCATTTGAATTGGAGGT+TGG 0.512037 7:-103261548 None:intergenic
TCAGTCTTCTGATTCTCCAA+TGG 0.512323 7:+103261595 MsaT040722.1:CDS
TTTAAAACTCCACGGAAGCC+AGG 0.512523 7:-103245258 None:intergenic
GCGTGCTGTCACGACCCAAC+AGG 0.513803 7:+103248016 MsaT040722.1:CDS
TTGTTGTGAATAAGAAGAAA+AGG 0.514743 7:+103261420 MsaT040722.1:CDS
TCCACGGAAGCCAGGAAGGT+TGG 0.519048 7:-103245250 None:intergenic
GAAAATTGGCCAGAAAATGC+TGG 0.523262 7:+103246422 MsaT040722.1:CDS
GTTCTTGTTTCAGAGTGTCC+AGG 0.523822 7:+103245588 MsaT040722.1:intron
CTTAGTTGCTGAAACGGCCG+TGG 0.523904 7:+103249148 MsaT040722.1:CDS
CAAAAGAAATAGCTATGTAA+AGG 0.523910 7:+103250588 MsaT040722.1:CDS
GAATCGATGATCAACGGTGG+TGG 0.526899 7:-103260250 None:intergenic
TTCTTCATCTTATGCGACAA+TGG 0.529849 7:+103257111 MsaT040722.1:CDS
TCTAAATCTGTCTCTACCAA+TGG 0.530928 7:-103256887 None:intergenic
GAATAAGAAGAAAAGGTCAA+TGG 0.531680 7:+103261427 MsaT040722.1:CDS
TGAATCCTTTAAGTCTCTTG+CGG 0.531699 7:+103245666 MsaT040722.1:CDS
GAAGTTGTTTGGTATTAAAG+TGG 0.532395 7:+103248209 MsaT040722.1:CDS
CGTTGATCATCGATTCCAGC+TGG 0.533603 7:+103260257 MsaT040722.1:CDS
GCAGTAAGCTAGCTGAGTTT+GGG 0.534684 7:+103260448 MsaT040722.1:intron
CAAGATCTTTATCAAGGTGG+TGG 0.535018 7:+103260585 MsaT040722.1:CDS
AATGAAGCCATGCCGTCCTA+AGG 0.537439 7:-103248577 None:intergenic
GGTCAACTTGAGAGCAGTAT+GGG 0.537715 7:-103257077 None:intergenic
CCATTATTGTAGGCACAACA+AGG 0.538540 7:-103261160 None:intergenic
AAGTGTCCCAGCAACATCAT+TGG 0.539742 7:-103249108 None:intergenic
AGGAAGGTTGGAAATGTAGC+AGG 0.540849 7:-103245238 None:intergenic
ACTCTGTGTCACTTGAGCTA+GGG 0.543713 7:-103261118 None:intergenic
CATCGACTGTGTCAGTGACC+AGG 0.548446 7:-103246277 None:intergenic
ATCCGTGTGAGATTGAATTC+TGG 0.549735 7:+103246323 MsaT040722.1:CDS
AGATGAAGAAGGAAGAGTAA+AGG 0.549861 7:-103257098 None:intergenic
GATCAAACTTACCCTGGCAT+AGG 0.550095 7:-103255043 None:intergenic
TGATGACGACAAGGTGATGA+TGG 0.550605 7:+103260710 MsaT040722.1:CDS
TCTGTGCCCACTCATCGCAT+TGG 0.551760 7:+103248847 MsaT040722.1:CDS
ATAGTTATCAAAGCTATTTG+TGG 0.553588 7:+103250191 MsaT040722.1:CDS
AGTCTTGAGAGCTGGAGTCA+TGG 0.554121 7:-103257054 None:intergenic
TCGTATTTATACTACCCCAT+TGG 0.554200 7:-103261611 None:intergenic
GATGTCATCCTTCCTATGCC+AGG 0.554785 7:+103255031 MsaT040722.1:CDS
TGGACATATGCTTGGCGTCA+AGG 0.557654 7:+103247291 MsaT040722.1:CDS
ACCTTTGCTTTAAAACTCCA+CGG 0.557959 7:-103245266 None:intergenic
TCTATGGAAGAACACGATGT+TGG 0.562361 7:+103245210 MsaT040722.1:CDS
ACAAGTAGTATTAGGAGACT+TGG 0.563868 7:+103250444 MsaT040722.1:CDS
TCCAACCTTCCTGGCTTCCG+TGG 0.564068 7:+103245249 MsaT040722.1:CDS
GACTGATACTAACCGTCCGA+TGG 0.567131 7:-103245792 None:intergenic
TACTGATAGTAATAAGCCAT+TGG 0.567717 7:+103256871 MsaT040722.1:CDS
AGTATGGGAGTCTTGAGAGC+TGG 0.568108 7:-103257062 None:intergenic
AGCTTGCTAGAGAGGAAGCA+TGG 0.569276 7:+103260883 MsaT040722.1:CDS
AAGATACCAATGATGTTGCT+GGG 0.573340 7:+103249102 MsaT040722.1:CDS
GCAGATTCCTTAGGACGGCA+TGG 0.576928 7:+103248570 MsaT040722.1:CDS
ATTTGGATTTGCGGGTACAA+AGG 0.577304 7:+103247989 MsaT040722.1:CDS
TCCCAGATCAACTCATCACA+TGG 0.578469 7:+103260335 MsaT040722.1:CDS
GAGCTCTGATGATGCTCCAA+AGG 0.578873 7:+103257003 MsaT040722.1:CDS
AAGTTGTTTGGTATTAAAGT+GGG 0.579913 7:+103248210 MsaT040722.1:CDS
TAACACTTGTAGTAGACTTG+AGG 0.580589 7:-103261238 None:intergenic
TGCGGCTCCAATGCGATGAG+TGG 0.581485 7:-103248854 None:intergenic
GAATCAGAAGACTGAGCTTG+AGG 0.581830 7:-103261587 None:intergenic
TTGAACAAATATCTAGCCAC+AGG 0.583846 7:+103260993 MsaT040722.1:CDS
AGGCACAACAAGGGTAGAAG+TGG 0.586674 7:-103261150 None:intergenic
ACTGATACTAACCGTCCGAT+GGG 0.586844 7:-103245791 None:intergenic
ATCTGGGAAGAAATTGTGAA+TGG 0.587163 7:-103260320 None:intergenic
CCCATGTGATGAGTTGATCT+GGG 0.588059 7:-103260336 None:intergenic
TTTGATTTAAGATTGTTACA+AGG 0.590503 7:-103261062 None:intergenic
GCTTGCAGCAGTGGAATCGA+AGG 0.594762 7:+103245456 MsaT040722.1:CDS
TTGGAGCCGCATTACTCCGG+GGG 0.597347 7:+103248866 MsaT040722.1:CDS
AAGCTTAGATTCCTCAGCAA+GGG 0.597669 7:-103245437 None:intergenic
GCGGCTCCAATGCGATGAGT+GGG 0.599315 7:-103248853 None:intergenic
TTGAAGATCACTAACAAACC+GGG 0.601696 7:-103260565 None:intergenic
AAACTCCACGGAAGCCAGGA+AGG 0.602329 7:-103245254 None:intergenic
AAACTTACCCTGGCATAGGA+AGG 0.605512 7:-103255039 None:intergenic
CTCAGCAAGGGCTTCAAGTG+AGG 0.605993 7:-103245425 None:intergenic
CAAGCTTAGATTCCTCAGCA+AGG 0.606287 7:-103245438 None:intergenic
TCAGAAGACTGAGCTTGAGG+TGG 0.606482 7:-103261584 None:intergenic
CTCACTTGAAGCCCTTGCTG+AGG 0.608156 7:+103245426 MsaT040722.1:CDS
CTGGTCACTGACACAGTCGA+TGG 0.608501 7:+103246278 MsaT040722.1:CDS
ATCCTTTAAGTCTCTTGCGG+GGG 0.609691 7:+103245669 MsaT040722.1:CDS
CCTGTAACTTCCAGTAACAC+TGG 0.611942 7:-103256692 None:intergenic
ATAGTAATAAGGGACCTTTG+TGG 0.612114 7:+103261291 MsaT040722.1:CDS
GTGGCCAAGAGATGAAGTGT+TGG 0.612713 7:+103261205 MsaT040722.1:CDS
AGGTGTTGTTGCAGATTCTG+TGG 0.615804 7:+103248530 MsaT040722.1:CDS
ATGTCATCCTTCCTATGCCA+GGG 0.617570 7:+103255032 MsaT040722.1:CDS
ATTAATCAAATTAGTGCTGG+CGG 0.620417 7:+103245721 MsaT040722.1:CDS
CACCAGAATTCAATCTCACA+CGG 0.620819 7:-103246325 None:intergenic
CTTCAAGATCTTTATCAAGG+TGG 0.622674 7:+103260582 MsaT040722.1:CDS
TCACATGGGACCATGTCTCA+AGG 0.623811 7:+103260350 MsaT040722.1:CDS
GATTGAAAGGATGAACATGA+TGG 0.625916 7:+103260917 MsaT040722.1:CDS
AATTAGAGGATAGGAGAAGG+TGG 0.625991 7:+103261186 MsaT040722.1:CDS
TTCTGATAAATCCCATCGGA+CGG 0.626859 7:+103245780 MsaT040722.1:CDS
TTGTGGGAGAGAATCTCTGA+AGG 0.627055 7:+103261308 MsaT040722.1:CDS
CGTGTGAGATTGAATTCTGG+TGG 0.627135 7:+103246326 MsaT040722.1:CDS
TAAGGAGAACAAAGATACCC+AGG 0.627641 7:+103247252 MsaT040722.1:CDS
TAATTACGTACCTTGAGACA+TGG 0.636193 7:-103260360 None:intergenic
AATCCTTTAAGTCTCTTGCG+GGG 0.637072 7:+103245668 MsaT040722.1:CDS
ATAATGATAATAATTTCCCC+CGG 0.637802 7:+103260547 MsaT040722.1:CDS
CAGCTGGAATCGATGATCAA+CGG 0.638993 7:-103260256 None:intergenic
ATTGTCGCATAAGATGAAGA+AGG 0.639357 7:-103257109 None:intergenic
CTGCTGCAGATTCCTTAGGA+CGG 0.641421 7:+103248565 MsaT040722.1:CDS
AAGATACCCAGGAAGCTTTG+GGG 0.643057 7:+103247263 MsaT040722.1:CDS
TCATGGACAGATTCATCATG+TGG 0.643774 7:-103257037 None:intergenic
AGATACCCAGGAAGCTTTGG+GGG 0.654854 7:+103247264 MsaT040722.1:CDS
GTGTTACTGGAAGTTACAGG+AGG 0.657511 7:+103256695 MsaT040722.1:CDS
TCAGAGATTCTCTCCCACAA+AGG 0.658578 7:-103261305 None:intergenic
ATTGGAGCCGCATTACTCCG+GGG 0.660145 7:+103248865 MsaT040722.1:CDS
TGAAGATCACTAACAAACCG+GGG 0.660207 7:-103260564 None:intergenic
GTTTCAGCAACTAAGAATTG+GGG 0.674354 7:-103249140 None:intergenic
TGTTACTGGAAGTTACAGGA+GGG 0.674780 7:+103256696 MsaT040722.1:CDS
CGGATTCTGATAAATCCCAT+CGG 0.680035 7:+103245776 MsaT040722.1:CDS
CCCAGATCAACTCATCACAT+GGG 0.681423 7:+103260336 MsaT040722.1:CDS
CATTATTGTAGGCACAACAA+GGG 0.683728 7:-103261159 None:intergenic
TCTGGTGGATCCAACAACAA+CGG 0.703810 7:+103246341 MsaT040722.1:CDS
GATACAAGAGAAGTGCAAAG+AGG 0.707168 7:+103261345 MsaT040722.1:CDS
TGGAGCCGCATTACTCCGGG+GGG 0.716731 7:+103248867 MsaT040722.1:CDS
AGTCTTCTGATTCTCCAATG+GGG 0.718372 7:+103261597 MsaT040722.1:CDS
GAAGATCACTAACAAACCGG+GGG 0.720209 7:-103260563 None:intergenic
CTGGAATCGATGATCAACGG+TGG 0.722514 7:-103260253 None:intergenic
AGAAGAAGAAGAATATCCTG+TGG 0.722719 7:-103261009 None:intergenic
GAACAACGCAGCAAGTACAA+GGG 0.728444 7:+103250213 MsaT040722.1:CDS
ACACTGTGATGATGACGACA+AGG 0.746516 7:+103260701 MsaT040722.1:CDS
CCTGAATGTCATGATGACTG+CGG 0.779537 7:+103250514 MsaT040722.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 103245204 103261622 103245204 ID=MsaG040722
Chr7 mRNA 103245204 103261622 103245204 ID=MsaT040722.1;Parent=MsaG040722
Chr7 exon 103245204 103245286 103245204 ID=MsaT040722.1.exon1;Parent=MsaT040722.1
Chr7 CDS 103245204 103245286 103245204 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103245369 103245477 103245369 ID=MsaT040722.1.exon2;Parent=MsaT040722.1
Chr7 CDS 103245369 103245477 103245369 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103245601 103245801 103245601 ID=MsaT040722.1.exon3;Parent=MsaT040722.1
Chr7 CDS 103245601 103245801 103245601 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103246242 103246456 103246242 ID=MsaT040722.1.exon4;Parent=MsaT040722.1
Chr7 CDS 103246242 103246456 103246242 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103247240 103247312 103247240 ID=MsaT040722.1.exon5;Parent=MsaT040722.1
Chr7 CDS 103247240 103247312 103247240 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103247981 103248037 103247981 ID=MsaT040722.1.exon6;Parent=MsaT040722.1
Chr7 CDS 103247981 103248037 103247981 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103248162 103248241 103248162 ID=MsaT040722.1.exon7;Parent=MsaT040722.1
Chr7 CDS 103248162 103248241 103248162 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103248328 103248390 103248328 ID=MsaT040722.1.exon8;Parent=MsaT040722.1
Chr7 CDS 103248328 103248390 103248328 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103248530 103248617 103248530 ID=MsaT040722.1.exon9;Parent=MsaT040722.1
Chr7 CDS 103248530 103248617 103248530 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103248832 103248903 103248832 ID=MsaT040722.1.exon10;Parent=MsaT040722.1
Chr7 CDS 103248832 103248903 103248832 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103249086 103249169 103249086 ID=MsaT040722.1.exon11;Parent=MsaT040722.1
Chr7 CDS 103249086 103249169 103249086 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103249321 103249394 103249321 ID=MsaT040722.1.exon12;Parent=MsaT040722.1
Chr7 CDS 103249321 103249394 103249321 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103250183 103250247 103250183 ID=MsaT040722.1.exon13;Parent=MsaT040722.1
Chr7 CDS 103250183 103250247 103250183 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103250437 103250609 103250437 ID=MsaT040722.1.exon14;Parent=MsaT040722.1
Chr7 CDS 103250437 103250609 103250437 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103254986 103255053 103254986 ID=MsaT040722.1.exon15;Parent=MsaT040722.1
Chr7 CDS 103254986 103255053 103254986 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103255177 103255237 103255177 ID=MsaT040722.1.exon16;Parent=MsaT040722.1
Chr7 CDS 103255177 103255237 103255177 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103256518 103256555 103256518 ID=MsaT040722.1.exon17;Parent=MsaT040722.1
Chr7 CDS 103256518 103256555 103256518 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103256678 103256746 103256678 ID=MsaT040722.1.exon18;Parent=MsaT040722.1
Chr7 CDS 103256678 103256746 103256678 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103256856 103257165 103256856 ID=MsaT040722.1.exon19;Parent=MsaT040722.1
Chr7 CDS 103256856 103257165 103256856 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103260241 103260371 103260241 ID=MsaT040722.1.exon20;Parent=MsaT040722.1
Chr7 CDS 103260241 103260371 103260241 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Chr7 exon 103260452 103261622 103260452 ID=MsaT040722.1.exon21;Parent=MsaT040722.1
Chr7 CDS 103260452 103261622 103260452 ID=cds.MsaT040722.1;Parent=MsaT040722.1
Gene Sequence

>MsaT040722.1

ATGAATTCTATGGAAGAACACGATGTTGGAATCACCTGCTACATTTCCAACCTTCCTGGCTTCCGTGGAGTTTTAAAGCAAAGGTATTCTGATTTTATTGTACATGAAGTAGATTTAGATGGAAAAGTTGTTGAGTTGTCCTCACTTGAAGCCCTTGCTGAGGAATCTAAGCTTGCAGCAGTGGAATCGAAGAGTGTCCAGGAAAATAAAACAGATACTACATCCGAAACTGAAGTTATAAGTTATGTTTCTCAAATTGAATCCTTTAAGTCTCTTGCGGGGGATTCTGATGCGTCTAGTTTGGAAGAATTTATTAATCAAATTAGTGCTGGCGGTGAAGATGATATCTCTCCGATTACTCTTTCTCCGGATTCTGATAAATCCCATCGGACGGCAGTGCATGTTTTCTTTAGGGAAAACTTCAAGTTCCTGGTCACTGACACAGTCGATGGACCTGATGCTTCCACAAAATGCATCCGTGTGAGATTGAATTCTGGTGGATCCAACAACAACGGCAAAAACTCTAGGAAACGCAAAGAAAGAGATACCAAGCCTTTTGACAGTAGAGGTTCTGAAAATTGGCCAGAAAATGCTGGCAAGTTCCTTAGGTTTCACCTTTATAAGGAGAACAAAGATACCCAGGAAGCTTTGGGGGTGCTTGGACATATGCTTGGCGTCAAGTCGAGATCATTTGGATTTGCGGGTACAAAGGATAAGCGTGCTGTCACGACCCAACAGGTTACTGTTTATAAGAAGCAAGCAAGCAAACTAGCTTCCCTTAATGAGAAGTTGTTTGGTATTAAAGTGGGTGATTTCTGTTATGTTAATGAAGGACTCTGTCTTGGTCAGCTATTCGGAAACAGATTTACAATCACATTACGAGGTGTTGTTGCAGATTCTGTGGATACCATAAAAGCTGCTGCAGATTCCTTAGGACGGCATGGCTTCATTAACTACTTTGGTTTACAACGTTTTGGAAGTGGTTCTGTGCCCACTCATCGCATTGGAGCCGCATTACTCCGGGGGGAATGGAAATGTGCTGCACGCAAGTACTATAAAGATACCAATGATGTTGCTGGGACACTTAAGCAGTTGCCCCAATTCTTAGTTGCTGAAACGGCCGTGCTGAAAAGTTTAAAACAAAATCCTGGAAACTACTTGCAGGCTTTGAAATCTATTCCACGAACTTTAAGGATGATGTATGTGCATAGTTATCAAAGCTATTTGTGGAACAACGCAGCAAGTACAAGGGTTAAAAAATATGGAACTGAACAAGTAGTATTAGGAGACTTGGTATACTGTAAAGAAAATCCTTCCGAAAAAGTTACAGGGATTGTTGGACCTGAATGTCATGATGACTGCGGCGATAGTTATGATACTAATAACGAAGATGAAGTCTCTGGAGATATTCATGACAAAAGAAATAGCTATGTAAAGGCTGTGACCACTGAAGATCTAAATTCAAAATGTTATGCAATTGATGATGTCATCCTTCCTATGCCAGGTTCACGGTCAATTTATCCAACCAATGATATTGTCAAAGTGTATAATGATTTGACAAGTAAAGATGGAATCAGTTTAACTGATAGCGTGCATAATGCAAAGGAATTCTCGATCACCAGTGTTACTGGAAGTTACAGGAGGGTTTTTCAGAAACCAATTGACTTTGAATGGGAATTACTTACATATACTGATAGTAATAAGCCATTGGTAGAGACAGATTTAGATAAAATTGGAAAGTCTAAACCTATAATTGTCAACACAGTGCGTGCACTAAATGAGCAAAAGGAGAAAGTCTTTGATTGCATTAGCCAATTAGAGAGCTCTGATGATGCTCCAAAGGAAGTAATATTACCACATGATGAATCTGTCCATGACTCCAGCTCTCAAGACTCCCATACTGCTCTCAAGTTGACCTTTACTCTTCCTTCTTCATCTTATGCGACAATGGCGATAAGAGAGCTTCTGAAGACTTCAACATCTACCCAAACTCCACCACCGTTGATCATCGATTCCAGCTGGACTAACGACGAACTTCTTCTACTCTTCAAGATCACATCTACCATTCACAATTTCTTCCCAGATCAACTCATCACATGGGACCATGTCTCAAGTAAGCTAGCTGAGTTTGGGATCAAGAAGAGTGCTCAAAATTGTAAGGAGAAGTTTGAACATGAGAACACATCATTTATGAAGATTCATAACAATCATAATGATAATAATTTCCCCCGGTTTGTTAGTGATCTTCAAGATCTTTATCAAGGTGGTGGTGTTAGTGTTGATAATAAACAACCAAACCCTGACGAAAACAAAAATACCCAACACGAAGAAGATGATTCGAGAGATGATAGAGTAGAGACCAAACACTGTGATGATGACGACAAGGTGATGATGGAGAAATCAAAGAACAAAAAGAGGAAGAGGCGCCTTGATAGATTTGAAATGTTGAAGAGTTTTTGTGAGACAGTGGTTAACAAAATAATTGCACAACAAGAAGAAATACACAATAAGCTACTTGAAGACATGCTCAAAAGAGATCAAGAAAAGCTTGCTAGAGAGGAAGCATGGAAAAAACAAGAGATTGAAAGGATGAACATGATGGTACAACAACAACAAGCTATTGCAAGTGATAGACAAGCCAACATCATTGAATTCTTGAACAAATATCTAGCCACAGGATATTCTTCTTCTTCTTCACAATTGCACTCACAAAACCCTAATAATCCTTGTAACAATCTTAAATCAAAAACACCTTTTTCTAATGTGATAGCAGATCAAAACCCTAGCTCAAGTGACACAGAGTACTCAAATTCCACTTCTACCCTTGTTGTGCCTACAATAATGGAGAAATTAGAGGATAGGAGAAGGTGGCCAAGAGATGAAGTGTTGGCACTCATAAACCTCAAGTCTACTACAAGTGTTACAAACAGAAGCAACAATAATGTTGAAGGAAATAGTAATAAGGGACCTTTGTGGGAGAGAATCTCTGAAGGAATGTTTGAGTTGGGATACAAGAGAAGTGCAAAGAGGTGTAAAGAAAAATGGGAAAACATAAATAAGTATTTTAAAAAGACAAAGGATATTGTTGTGAATAAGAAGAAAAGGTCAATGGATTCAAGGACTTGTCCTTATTTTCATCAACTCAGTTCTTTGTATAATCAGCAGCAGCAGCAGCAGGGAAAAATAATTGATCCTCAATTAACCAAACATCCAACCTCCAATTCAAATGGTCATATTGTAGATGATCCACCTCAAGCTCAGTCTTCTGATTCTCCAATGGGGTAG
Protein sequence

>MsaT040722.1

MNSMEEHDVGITCYISNLPGFRGVLKQRYSDFIVHEVDLDGKVVELSSLEALAEESKLAAVESKSVQENKTDTTSETEVISYVSQIESFKSLAGDSDASSLEEFINQISAGGEDDISPITLSPDSDKSHRTAVHVFFRENFKFLVTDTVDGPDASTKCIRVRLNSGGSNNNGKNSRKRKERDTKPFDSRGSENWPENAGKFLRFHLYKENKDTQEALGVLGHMLGVKSRSFGFAGTKDKRAVTTQQVTVYKKQASKLASLNEKLFGIKVGDFCYVNEGLCLGQLFGNRFTITLRGVVADSVDTIKAAADSLGRHGFINYFGLQRFGSGSVPTHRIGAALLRGEWKCAARKYYKDTNDVAGTLKQLPQFLVAETAVLKSLKQNPGNYLQALKSIPRTLRMMYVHSYQSYLWNNAASTRVKKYGTEQVVLGDLVYCKENPSEKVTGIVGPECHDDCGDSYDTNNEDEVSGDIHDKRNSYVKAVTTEDLNSKCYAIDDVILPMPGSRSIYPTNDIVKVYNDLTSKDGISLTDSVHNAKEFSITSVTGSYRRVFQKPIDFEWELLTYTDSNKPLVETDLDKIGKSKPIIVNTVRALNEQKEKVFDCISQLESSDDAPKEVILPHDESVHDSSSQDSHTALKLTFTLPSSSYATMAIRELLKTSTSTQTPPPLIIDSSWTNDELLLLFKITSTIHNFFPDQLITWDHVSSKLAEFGIKKSAQNCKEKFEHENTSFMKIHNNHNDNNFPRFVSDLQDLYQGGGVSVDNKQPNPDENKNTQHEEDDSRDDRVETKHCDDDDKVMMEKSKNKKRKRRLDRFEMLKSFCETVVNKIIAQQEEIHNKLLEDMLKRDQEKLAREEAWKKQEIERMNMMVQQQQAIASDRQANIIEFLNKYLATGYSSSSSQLHSQNPNNPCNNLKSKTPFSNVIADQNPSSSDTEYSNSTSTLVVPTIMEKLEDRRRWPRDEVLALINLKSTTSVTNRSNNNVEGNSNKGPLWERISEGMFELGYKRSAKRCKEKWENINKYFKKTKDIVVNKKKRSMDSRTCPYFHQLSSLYNQQQQQQGKIIDPQLTKHPTSNSNGHIVDDPPQAQSSDSPMG*