AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040757

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040757 MtrunA17_Chr7g0274471 93.651 441 15 2 1 428 1 441 0.0 837
MsaG040757 MtrunA17_Chr1g0190301 53.695 406 123 10 47 426 19 385 2.66e-126 370
MsaG040757 MtrunA17_Chr7g0261911 42.686 417 194 9 1 377 26 437 9.16e-89 278
MsaG040757 MtrunA17_Chr1g0175091 52.542 177 70 5 192 364 247 413 3.23e-50 175
MsaG040757 MtrunA17_Chr7g0258551 53.147 143 66 1 204 346 211 352 5.46e-48 168
MsaG040757 MtrunA17_Chr2g0291021 54.362 149 66 1 205 351 49 197 1.75e-47 166
MsaG040757 MtrunA17_Chr7g0254981 44.382 178 89 2 171 338 9 186 7.28e-46 160
MsaG040757 MtrunA17_Chr6g0461641 51.974 152 57 3 201 336 38 189 4.37e-41 150
MsaG040757 MtrunA17_Chr5g0417521 36.979 192 97 4 201 370 11 200 3.65e-35 133
MsaG040757 MtrunA17_Chr8g0356501 36.170 188 105 4 191 366 23 207 1.54e-34 131
MsaG040757 MtrunA17_Chr5g0409731 48.936 141 60 3 205 333 25 165 4.47e-34 129
MsaG040757 MtrunA17_Chr2g0321461 42.767 159 70 3 201 338 41 199 5.32e-34 130
MsaG040757 MtrunA17_Chr4g0042451 38.144 194 93 5 201 370 13 203 1.55e-33 128
MsaG040757 MtrunA17_Chr2g0310011 40.690 145 77 2 190 328 20 161 1.12e-31 123
MsaG040757 MtrunA17_Chr1g0175071 40.252 159 92 2 179 337 153 308 3.18e-30 119
MsaG040757 MtrunA17_Chr2g0290991 40.136 147 76 1 205 339 39 185 1.20e-28 114
MsaG040757 MtrunA17_Chr1g0197171 38.889 144 79 1 205 339 44 187 7.38e-28 112
MsaG040757 MtrunA17_Chr7g0242521 43.511 131 60 3 205 327 13 137 1.91e-27 109
MsaG040757 MtrunA17_Chr7g0258531 40.625 160 78 5 177 330 191 339 8.88e-24 101
MsaG040757 MtrunA17_Chr1g0175081 55.405 74 31 1 192 265 11 82 2.95e-23 93.6
MsaG040757 MtrunA17_Chr1g0162931 67.857 56 18 0 206 261 19 74 1.18e-19 88.6
MsaG040757 MtrunA17_Chr2g0288601 63.793 58 21 0 206 263 72 129 1.80e-19 84.0
MsaG040757 MtrunA17_Chr1g0210911 66.071 56 19 0 206 261 23 78 3.31e-19 88.2
MsaG040757 MtrunA17_Chr3g0095611 57.895 57 24 0 205 261 19 75 1.35e-18 84.0
MsaG040757 MtrunA17_Chr2g0320231 60.294 68 26 1 206 273 98 164 1.74e-18 86.7
MsaG040757 MtrunA17_Chr8g0379121 53.247 77 32 1 206 278 112 188 2.51e-18 86.7
MsaG040757 MtrunA17_Chr7g0243081 62.500 56 21 0 206 261 72 127 2.86e-18 85.9
MsaG040757 MtrunA17_Chr8g0366281 40.945 127 64 2 199 325 167 282 8.26e-18 84.3
MsaG040757 MtrunA17_Chr6g0479431 56.164 73 30 1 206 276 203 275 1.27e-17 84.0
MsaG040757 MtrunA17_Chr1g0154261 66.071 56 19 0 206 261 127 182 1.42e-17 82.8
MsaG040757 MtrunA17_Chr5g0410741 61.667 60 23 0 202 261 216 275 1.38e-16 81.3
MsaG040757 MtrunA17_Chr3g0109981 61.667 60 23 0 202 261 271 330 2.43e-16 80.9
MsaG040757 MtrunA17_Chr2g0283951 68.000 50 16 0 206 255 90 139 1.02e-15 78.6
MsaG040757 MtrunA17_Chr2g0304841 54.930 71 30 2 201 271 209 277 1.60e-15 79.0
MsaG040757 MtrunA17_Chr7g0275401 66.667 54 17 1 205 258 192 244 2.27e-15 76.3
MsaG040757 MtrunA17_Chr4g0067981 46.739 92 40 2 205 288 215 305 1.85e-14 75.5
MsaG040757 MtrunA17_Chr3g0137101 60.345 58 22 1 201 258 194 250 1.01e-13 73.2
MsaG040757 MtrunA17_Chr4g0009901 53.030 66 28 2 205 270 168 230 1.41e-13 70.9
MsaG040757 MtrunA17_Chr1g0198601 54.839 62 28 0 205 266 211 272 1.62e-13 71.6
MsaG040757 MtrunA17_Chr4g0045361 62.963 54 20 0 205 258 274 327 1.75e-13 72.0
MsaG040757 MtrunA17_Chr4g0009981 50.649 77 34 3 194 270 106 178 3.60e-13 69.3
MsaG040757 MtrunA17_Chr4g0062921 53.030 66 31 0 199 264 229 294 3.79e-13 70.9
MsaG040757 MtrunA17_Chr1g0149841 53.623 69 31 1 201 269 187 254 4.06e-13 70.9
MsaG040757 MtrunA17_Chr4g0031961 51.562 64 30 1 195 258 121 183 7.33e-13 68.9
MsaG040757 MtrunA17_Chr1g0161431 62.963 54 19 1 205 258 200 252 8.16e-13 70.5
MsaG040757 MtrunA17_Chr4g0074401 51.562 64 30 1 201 264 199 261 8.69e-13 70.1
MsaG040757 MtrunA17_Chr1g0149831 57.627 59 21 1 200 258 123 177 1.27e-12 67.0
MsaG040757 MtrunA17_Chr4g0074411 53.125 64 29 1 201 264 197 259 1.42e-12 69.7
MsaG040757 MtrunA17_Chr5g0422621 58.929 56 23 0 203 258 196 251 1.53e-12 68.6
MsaG040757 MtrunA17_Chr4g0074421 55.357 56 24 1 200 255 128 182 3.27e-12 65.5
MsaG040757 MtrunA17_Chr5g0402631 57.895 57 24 0 202 258 211 267 4.15e-12 67.0
MsaG040757 MtrunA17_Chr2g0294911 54.237 59 26 1 200 258 206 263 7.29e-12 67.4
MsaG040757 MtrunA17_Chr4g0054981 37.895 95 58 1 164 258 269 362 1.76e-11 65.9
MsaG040757 MtrunA17_Chr7g0255711 53.704 54 24 1 205 258 91 143 3.71e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040757 AT5G29000.2 45.263 380 160 9 3 349 14 378 4.67e-85 266
MsaG040757 AT5G29000.1 46.608 339 131 7 52 349 6 335 8.28e-82 256
MsaG040757 AT5G29000.3 46.608 339 131 7 52 349 6 335 8.28e-82 256
MsaG040757 AT3G04450.4 49.164 299 116 7 93 366 71 358 7.25e-80 252
MsaG040757 AT3G04450.2 49.164 299 116 7 93 366 71 358 7.25e-80 252
MsaG040757 AT3G04450.1 49.164 299 116 7 93 366 111 398 1.55e-79 253
MsaG040757 AT4G28610.1 51.190 252 99 8 106 334 105 355 1.88e-69 226
MsaG040757 AT2G20400.1 39.815 324 159 9 44 334 40 360 7.59e-52 179
MsaG040757 AT2G20400.2 40.000 325 158 10 44 334 40 361 1.11e-50 176
MsaG040757 AT2G01060.1 48.603 179 86 2 204 381 14 187 1.08e-48 167
MsaG040757 AT3G13040.3 51.397 179 74 2 197 362 233 411 1.59e-46 166
MsaG040757 AT3G13040.1 51.397 179 74 2 197 362 233 411 1.59e-46 166
MsaG040757 AT3G13040.2 51.397 179 74 2 197 362 233 411 1.59e-46 166
MsaG040757 AT5G06800.1 49.405 168 79 2 174 336 156 322 3.49e-46 164
MsaG040757 AT5G06800.2 48.256 172 80 2 174 336 156 327 2.36e-45 161
MsaG040757 AT3G04450.3 45.226 199 84 4 93 271 111 304 7.19e-45 158
MsaG040757 AT5G29000.4 38.538 301 140 8 3 273 14 299 2.06e-44 157
MsaG040757 AT3G24120.1 44.444 171 83 3 193 351 29 199 6.38e-40 145
MsaG040757 AT4G13640.1 51.408 142 55 3 205 333 37 177 1.57e-39 144
MsaG040757 AT3G24120.2 43.678 174 83 4 193 351 29 202 8.23e-38 139
MsaG040757 AT4G13640.2 50.345 145 55 4 205 333 37 180 1.88e-37 138
MsaG040757 AT1G69580.1 43.750 160 73 1 194 336 23 182 9.07e-37 137
MsaG040757 AT3G04030.3 39.773 176 91 2 193 353 33 208 2.93e-36 137
MsaG040757 AT1G69580.2 44.099 161 72 4 194 336 23 183 2.38e-35 134
MsaG040757 AT5G18240.1 45.570 158 62 4 201 338 41 194 8.84e-35 133
MsaG040757 AT5G18240.4 45.570 158 62 4 201 338 41 194 8.84e-35 133
MsaG040757 AT1G79430.2 43.791 153 77 3 190 336 19 168 1.92e-34 132
MsaG040757 AT5G45580.1 34.043 235 123 3 205 421 7 227 2.87e-34 129
MsaG040757 AT5G45580.2 34.043 235 123 3 205 421 23 243 3.46e-34 129
MsaG040757 AT3G04030.2 38.636 176 92 3 193 353 33 207 2.53e-33 129
MsaG040757 AT5G18240.5 44.937 158 61 5 201 338 41 192 8.19e-32 125
MsaG040757 AT3G04030.1 37.500 176 89 3 193 353 33 202 7.22e-31 122
MsaG040757 AT2G01060.2 44.755 143 73 2 240 381 1 138 5.20e-30 116
MsaG040757 AT5G18240.2 43.038 158 60 5 201 338 41 188 2.31e-29 118
MsaG040757 AT5G18240.3 43.038 158 60 5 201 338 41 188 2.31e-29 118
MsaG040757 AT3G12730.1 40.876 137 73 1 205 333 23 159 7.90e-29 113
MsaG040757 AT5G06800.3 49.020 102 47 1 174 270 156 257 8.96e-24 100
MsaG040757 AT4G04605.1 56.522 69 30 0 206 274 15 83 2.76e-19 86.7
MsaG040757 AT1G14600.1 67.857 56 18 0 206 261 24 79 4.23e-19 87.0
MsaG040757 AT2G42660.1 59.016 61 25 0 206 266 51 111 7.49e-19 86.3
MsaG040757 AT2G40260.1 54.321 81 34 2 189 269 67 144 6.68e-18 85.5
MsaG040757 AT5G42630.2 66.071 56 19 0 206 261 106 161 6.78e-18 82.8
MsaG040757 AT5G42630.1 66.071 56 19 0 206 261 106 161 1.42e-17 82.8
MsaG040757 AT2G02060.2 66.038 53 18 0 206 258 30 82 1.71e-17 82.4
MsaG040757 AT2G02060.1 66.038 53 18 0 206 258 30 82 1.71e-17 82.4
MsaG040757 AT2G38300.1 60.714 56 22 0 206 261 55 110 4.53e-17 82.4
MsaG040757 AT1G79430.1 41.509 106 53 3 237 336 1 103 5.04e-17 81.6
MsaG040757 AT4G17695.1 64.286 56 20 0 206 261 165 220 1.05e-16 81.3
MsaG040757 AT3G04030.5 35.606 132 70 2 237 353 1 132 1.15e-16 80.9
MsaG040757 AT1G32240.2 61.667 60 23 0 202 261 210 269 1.21e-16 81.6
MsaG040757 AT1G32240.1 61.667 60 23 0 202 261 210 269 1.64e-16 81.3
MsaG040757 AT5G16560.1 61.667 60 23 0 202 261 216 275 1.89e-16 81.3
MsaG040757 AT2G06020.1 55.738 61 27 0 201 261 82 142 4.12e-16 79.0
MsaG040757 AT4G04580.1 38.776 98 58 1 206 301 15 112 1.28e-15 74.7
MsaG040757 AT1G25550.1 56.061 66 29 0 202 267 206 271 6.04e-15 76.3
MsaG040757 AT2G03500.1 50.000 76 38 0 196 271 224 299 3.41e-14 74.7
MsaG040757 AT3G16857.3 68.519 54 16 1 205 258 167 219 8.25e-14 73.9
MsaG040757 AT3G16857.1 68.519 54 16 1 205 258 237 289 9.83e-14 73.6
MsaG040757 AT3G16857.2 68.519 54 16 1 205 258 237 289 9.94e-14 73.6
MsaG040757 AT4G37180.1 47.500 80 41 1 192 270 197 276 1.47e-13 72.0
MsaG040757 AT4G37180.2 47.500 80 41 1 192 270 204 283 1.69e-13 72.0
MsaG040757 AT3G62670.1 51.389 72 31 1 205 272 211 282 2.84e-13 71.6
MsaG040757 AT5G58080.1 56.667 60 25 1 199 258 188 246 3.79e-13 71.6
MsaG040757 AT1G68670.1 56.667 60 26 0 205 264 215 274 8.25e-13 69.7
MsaG040757 AT5G59570.2 52.459 61 28 1 198 258 134 193 1.02e-12 68.9
MsaG040757 AT5G59570.1 52.459 61 28 1 198 258 134 193 1.02e-12 68.9
MsaG040757 AT2G25180.1 59.322 59 23 1 200 258 190 247 1.28e-12 70.1
MsaG040757 AT5G44190.1 54.237 59 26 1 203 261 145 202 1.63e-12 68.9
MsaG040757 AT5G44190.2 54.237 59 26 1 203 261 145 202 1.77e-12 68.9
MsaG040757 AT2G20570.1 38.947 95 52 2 202 291 150 243 4.95e-12 67.8
MsaG040757 AT4G31920.1 53.226 62 28 1 202 263 180 240 5.53e-12 68.2
MsaG040757 AT1G49560.1 55.385 65 29 0 205 269 192 256 5.96e-12 67.0
MsaG040757 AT2G40970.1 42.667 75 42 1 184 258 84 157 6.07e-12 65.9
MsaG040757 AT3G46640.3 55.556 54 23 1 205 258 144 196 7.35e-12 66.6
MsaG040757 AT4G04555.1 49.180 61 30 1 206 266 15 74 7.85e-12 62.0
MsaG040757 AT3G46640.2 55.556 54 23 1 205 258 144 196 8.30e-12 66.6
MsaG040757 AT3G46640.1 55.556 54 23 1 205 258 144 196 8.30e-12 66.6
MsaG040757 AT1G67710.3 53.968 63 29 0 207 269 121 183 9.25e-12 67.0
MsaG040757 AT2G20570.2 40.000 90 48 2 207 291 171 259 9.30e-12 67.0
MsaG040757 AT1G25550.2 59.259 54 22 0 202 255 206 259 1.11e-11 65.5
MsaG040757 AT1G67710.2 53.968 63 29 0 207 269 130 192 1.15e-11 66.6
MsaG040757 AT1G67710.1 53.968 63 29 0 207 269 195 257 1.18e-11 67.0
MsaG040757 AT3G04030.4 32.576 132 68 3 237 353 1 126 1.20e-11 65.9
MsaG040757 AT5G05090.1 53.704 54 24 1 205 258 81 133 1.35e-11 65.1
MsaG040757 AT3G12730.2 32.381 105 63 1 237 333 1 105 1.82e-11 63.2
MsaG040757 AT3G10760.1 53.704 54 24 1 205 258 105 157 2.22e-11 65.1
MsaG040757 AT3G25790.1 36.090 133 72 3 141 264 127 255 2.47e-11 65.5
MsaG040757 AT2G01760.1 43.902 82 43 2 205 284 200 280 3.50e-11 65.1
MsaG040757 AT2G01760.3 43.902 82 43 2 205 284 135 215 3.55e-11 64.3
MsaG040757 AT2G01760.2 43.902 82 43 2 205 284 228 308 5.08e-11 64.7
MsaG040757 AT1G49190.1 46.377 69 34 1 205 270 215 283 6.46e-11 64.3
MsaG040757 AT4G37180.3 48.438 64 33 0 192 255 197 260 7.06e-11 63.2
MsaG040757 AT5G18240.6 36.441 118 53 5 237 338 1 112 8.31e-11 63.5

Find 96 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGTTCTTCCCAGTCTTA+TGG 0.207188 7:+103592314 MsaT040757.1:CDS
CTTTCTCCAGGTATGTCTTT+AGG 0.231387 7:-103594988 None:intergenic
CCAAGAAGCTGACTCCAATT+TGG 0.256047 7:-103592790 None:intergenic
ATTAGATTCAAGACACCTTT+TGG 0.275565 7:-103593460 None:intergenic
ATGATTCTGTTAATCCAAAT+TGG 0.276239 7:+103592776 MsaT040757.1:CDS
TCTGGACTCCAACGCATTCT+AGG 0.278668 7:-103593292 None:intergenic
TGGTGACTCAGGTTTGTATC+TGG 0.307622 7:-103593658 None:intergenic
CCAAATTGGAGTCAGCTTCT+TGG 0.308074 7:+103592790 MsaT040757.1:CDS
GATCCATTGCCAGATATTCT+TGG 0.312892 7:+103592636 MsaT040757.1:CDS
CCCTTCGCTTCTCAGGCATT+AGG 0.349271 7:+103592534 MsaT040757.1:CDS
TCAGGAAAACCAAGAATATC+TGG 0.353256 7:-103592645 None:intergenic
AGATTCTCCCTTCGCTTCTC+AGG 0.383362 7:+103592527 MsaT040757.1:CDS
ACAAAAGCTTCGTGAAGTTC+TGG 0.384632 7:-103593310 None:intergenic
TGCTGTGAAACGGGAGGAAA+AGG 0.393018 7:-103592489 None:intergenic
CTCCCGTTTCACAGCATGAT+AGG 0.396353 7:+103592496 MsaT040757.1:CDS
GAATTATGGTTACAGGAGTA+AGG 0.405297 7:+103594621 MsaT040757.1:intron
CAGAAGCAGCATACACCATC+TGG 0.414147 7:+103593208 MsaT040757.1:CDS
TCCATAAGACTGGGAAGAAC+AGG 0.416578 7:-103592315 None:intergenic
AATTGAGGAAATGAAATCTC+TGG 0.418669 7:+103594511 MsaT040757.1:CDS
ATAATTGCTTTCCATAAGAC+TGG 0.420183 7:-103592325 None:intergenic
TGCCTATCATGCTGTGAAAC+GGG 0.423721 7:-103592498 None:intergenic
GGAGGAAAAGGGACGCCGCC+AGG 0.438286 7:-103592477 None:intergenic
TTTGCTTTCTGCTTACATCA+TGG 0.449248 7:-103595014 None:intergenic
AAAGATGAACGGGATAAATC+AGG 0.451170 7:+103594949 MsaT040757.1:CDS
ATCTACCCTCTCATGTTCAT+TGG 0.454358 7:-103595058 None:intergenic
TTGCTTTCTGCTTACATCAT+GGG 0.456687 7:-103595013 None:intergenic
AGAATATCTGGCAATGGATC+TGG 0.459308 7:-103592633 None:intergenic
TGCACCTTCTGCTGCAGTGT+TGG 0.460592 7:+103594882 MsaT040757.1:CDS
GTTGATGCTGATGCTGAAGC+TGG 0.462318 7:-103593253 None:intergenic
CTGACGTGCCTCCAACAAAA+CGG 0.464112 7:+103595091 MsaT040757.1:CDS
GGTGAACGATGAAAAGAATC+CGG 0.468237 7:-103592357 None:intergenic
TGTTGTTGAACCAACACAAC+AGG 0.471739 7:+103592822 MsaT040757.1:CDS
TGACGTGCCTCCAACAAAAC+GGG 0.473833 7:+103595092 MsaT040757.1:CDS
ACATCATGGGAACTTTCTCC+AGG 0.474455 7:-103595000 None:intergenic
TGTATACCTTCTGGTGACTC+AGG 0.476063 7:-103593669 None:intergenic
TTGCCTATCATGCTGTGAAA+CGG 0.481947 7:-103592499 None:intergenic
TCATCTCAAGATGTTTCCCT+TGG 0.484323 7:-103594807 None:intergenic
TCAACCAATGATGAATGTCC+TGG 0.485207 7:+103592459 MsaT040757.1:CDS
CACGACGCCACCTTCTTCTC+CGG 0.487049 7:+103594906 MsaT040757.1:CDS
ATTAGGTGACAATGTGTCTT+CGG 0.494529 7:+103592551 MsaT040757.1:CDS
CATGCAGAGGCTACTCCAAA+AGG 0.496159 7:+103593445 MsaT040757.1:intron
TGATGTAAGCAGAAAGCAAA+TGG 0.496266 7:+103595017 MsaT040757.1:CDS
TTTGAGAAACAGAAACAGAT+AGG 0.496976 7:+103594832 MsaT040757.1:CDS
CAGCAGAAGGTGCATTTGAA+GGG 0.498153 7:-103594873 None:intergenic
AGGGAATCACTGAAGCATTA+CGG 0.500945 7:+103594641 MsaT040757.1:CDS
GCCGCCAGGACATTCATCAT+TGG 0.501664 7:-103592463 None:intergenic
GCAGCAGAAGGTGCATTTGA+AGG 0.504283 7:-103594874 None:intergenic
TTGAATCTAATGAATGTTGA+AGG 0.507075 7:+103593472 MsaT040757.1:CDS
AAATTGTCCACCGGAGAAGA+AGG 0.507969 7:-103594916 None:intergenic
GTGAACGATGAAAAGAATCC+GGG 0.513629 7:-103592356 None:intergenic
TCCATATATGAACCTGGAGG+TGG 0.532203 7:-103593519 None:intergenic
GAAGGTGCATTTGAAGGGGA+AGG 0.532963 7:-103594868 None:intergenic
AAACTAAGCCTAGAATGCGT+TGG 0.536261 7:+103593284 MsaT040757.1:CDS
ACCTAATGCCTGAGAAGCGA+AGG 0.539904 7:-103592535 None:intergenic
TACTCTCACTACCACCAAGC+TGG 0.543524 7:-103593345 None:intergenic
AGAAAATGATGATATATCGT+GGG 0.544394 7:+103592608 MsaT040757.1:CDS
GTGGAAGCAGTCAACCAGCT+TGG 0.551472 7:+103593331 MsaT040757.1:CDS
GATGTAGCTTGCAATGAGGC+AGG 0.552385 7:-103592402 None:intergenic
AATTATGGTTACAGGAGTAA+GGG 0.560396 7:+103594622 MsaT040757.1:intron
AAGAAAATGATGATATATCG+TGG 0.571936 7:+103592607 MsaT040757.1:CDS
TGAAGCATTACGGTTGCAGA+TGG 0.573866 7:+103594651 MsaT040757.1:CDS
CAGATTCAAATTGAAAACCA+AGG 0.574230 7:+103594790 MsaT040757.1:CDS
GAGAAACCAACCTGTTGTGT+TGG 0.577184 7:-103592832 None:intergenic
TTTGCTGTCAGACCCCTGAC+AGG 0.580082 7:-103592378 None:intergenic
AAACCAAGAATATCTGGCAA+TGG 0.585936 7:-103592639 None:intergenic
TAATTGTTATACAGATTCAA+AGG 0.586802 7:+103594761 MsaT040757.1:intron
GACGCCACCTTCTTCTCCGG+TGG 0.587703 7:+103594909 MsaT040757.1:CDS
TTTCATCGTTCACCTGTCAG+GGG 0.589580 7:+103592366 MsaT040757.1:CDS
GAAGCAGTCAACCAGCTTGG+TGG 0.594269 7:+103593334 MsaT040757.1:CDS
GCTGTGAAACGGGAGGAAAA+GGG 0.594959 7:-103592488 None:intergenic
AGATTCAAATTGAAAACCAA+GGG 0.608265 7:+103594791 MsaT040757.1:CDS
AGAACAGGTGAAGACGAAGA+AGG 0.608881 7:-103592300 None:intergenic
AGGTGAAGACGAAGAAGGCA+TGG 0.611132 7:-103592295 None:intergenic
TAATTGCTTTCCATAAGACT+GGG 0.614268 7:-103592324 None:intergenic
GTGTCCAACACTGCAGCAGA+AGG 0.618637 7:-103594886 None:intergenic
AAGTTACCAATGAACATGAG+AGG 0.619833 7:+103595052 MsaT040757.1:CDS
GCGACTTCATGAACAACTTG+AGG 0.620878 7:+103594684 MsaT040757.1:CDS
TCATCATTGGTTGAAAACAC+AGG 0.621388 7:-103592450 None:intergenic
AAGCAATTATATGAAACCCC+CGG 0.622265 7:+103592338 MsaT040757.1:CDS
TCGAACACTTCTGATCGGAT+AGG 0.622978 7:-103592427 None:intergenic
AAGGTGCATTTGAAGGGGAA+GGG 0.624076 7:-103594867 None:intergenic
GACATTCCTAAAGACATACC+TGG 0.630421 7:+103594982 MsaT040757.1:CDS
AGTTACCAATGAACATGAGA+GGG 0.634728 7:+103595053 MsaT040757.1:CDS
AGCAGAAGGTGCATTTGAAG+GGG 0.635905 7:-103594872 None:intergenic
ACAGGTCGAACACTTCTGAT+CGG 0.639592 7:-103592432 None:intergenic
AGATTGTTGACTTCTCCAGA+TGG 0.656610 7:-103593223 None:intergenic
GAAAATGATGATATATCGTG+GGG 0.660394 7:+103592609 MsaT040757.1:CDS
TACAAACCTGAGTCACCAGA+AGG 0.662240 7:+103593663 MsaT040757.1:CDS
CTATCATGCTGTGAAACGGG+AGG 0.666909 7:-103592495 None:intergenic
TTGTCCACCGGAGAAGAAGG+TGG 0.671117 7:-103594913 None:intergenic
TGAACGATGAAAAGAATCCG+GGG 0.672098 7:-103592355 None:intergenic
CCTAATGCCTGAGAAGCGAA+GGG 0.672541 7:-103592534 None:intergenic
ACCAATGATGAATGTCCTGG+CGG 0.684026 7:+103592462 MsaT040757.1:CDS
GGATGATGTAGCTTGCAATG+AGG 0.693225 7:-103592406 None:intergenic
GAGAAGTGAATGTTGCTGAG+TGG 0.714972 7:-103592578 None:intergenic
GAACGATGAAAAGAATCCGG+GGG 0.737762 7:-103592354 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 103592297 103595128 103592297 ID=MsaG040757
Chr7 mRNA 103592297 103595128 103592297 ID=MsaT040757.1;Parent=MsaG040757
Chr7 exon 103592297 103592692 103592297 ID=MsaT040757.1.exon1;Parent=MsaT040757.1
Chr7 CDS 103592297 103592692 103592297 ID=cds.MsaT040757.1;Parent=MsaT040757.1
Chr7 exon 103592724 103592843 103592724 ID=MsaT040757.1.exon2;Parent=MsaT040757.1
Chr7 CDS 103592724 103592843 103592724 ID=cds.MsaT040757.1;Parent=MsaT040757.1
Chr7 exon 103593193 103593364 103593193 ID=MsaT040757.1.exon3;Parent=MsaT040757.1
Chr7 CDS 103593193 103593364 103593193 ID=cds.MsaT040757.1;Parent=MsaT040757.1
Chr7 exon 103593452 103593528 103593452 ID=MsaT040757.1.exon4;Parent=MsaT040757.1
Chr7 CDS 103593452 103593528 103593452 ID=cds.MsaT040757.1;Parent=MsaT040757.1
Chr7 exon 103593645 103593684 103593645 ID=MsaT040757.1.exon5;Parent=MsaT040757.1
Chr7 CDS 103593645 103593684 103593645 ID=cds.MsaT040757.1;Parent=MsaT040757.1
Chr7 exon 103594486 103594543 103594486 ID=MsaT040757.1.exon6;Parent=MsaT040757.1
Chr7 CDS 103594486 103594543 103594486 ID=cds.MsaT040757.1;Parent=MsaT040757.1
Chr7 exon 103594636 103594705 103594636 ID=MsaT040757.1.exon7;Parent=MsaT040757.1
Chr7 CDS 103594636 103594705 103594636 ID=cds.MsaT040757.1;Parent=MsaT040757.1
Chr7 exon 103594775 103595128 103594775 ID=MsaT040757.1.exon8;Parent=MsaT040757.1
Chr7 CDS 103594775 103595128 103594775 ID=cds.MsaT040757.1;Parent=MsaT040757.1
Gene Sequence

>MsaT040757.1

ATGCCTTCTTCGTCTTCACCTGTTCTTCCCAGTCTTATGGAAAGCAATTATATGAAACCCCCGGATTCTTTTCATCGTTCACCTGTCAGGGGTCTGACAGCAAATCCTGCCTCATTGCAAGCTACATCATCCTATCCGATCAGAAGTGTTCGACCTGTGTTTTCAACCAATGATGAATGTCCTGGCGGCGTCCCTTTTCCTCCCGTTTCACAGCATGATAGGCAATATCAAGATTCTCCCTTCGCTTCTCAGGCATTAGGTGACAATGTGTCTTCGGAAATCCACTCAGCAACATTCACTTCTCACCTTCAAGAAAATGATGATATATCGTGGGGACCAGATCCATTGCCAGATATTCTTGGTTTTCCTGAAATTTTTTCTGTCCAGCATGATCAGACAATGTCAAAAACTGATTTTGGGGAGTGGGTTGAACAACTAATGACAAGTGATGATTCTGTTAATCCAAATTGGAGTCAGCTTCTTGGTGATGACAATGTTGTTGAACCAACACAACAGGCGATGCAGGTTTCTCAGAAGCAGCATACACCATCTGGAGAAGTCAACAATCTTTGTAATCCAGCTTCAGCATCAGCATCAACTGCTTCTCAAACTAAGCCTAGAATGCGTTGGAGTCCAGAACTTCACGAAGCTTTTGTGGAAGCAGTCAACCAGCTTGGTGGTAGTGAGAAGGCTACTCCAAAAGGTGTCTTGAATCTAATGAATGTTGAAGGCCTTACCATCTACCATGTAAAAAGCCACCTCCAGAAGTATAGAACTGCCAGATACAAACCTGAGTCACCAGAAGAAACTTCTGAGAAAAAGATGAGTTCAATTGAGGAAATGAAATCTCTGGACTTGAAAACGAGTAAGGGAATCACTGAAGCATTACGGTTGCAGATGGAACTTCAGAAGCGACTTCATGAACAACTTGAGATTCAAAGGAAGTTGCAGATTCAAATTGAAAACCAAGGGAAACATCTTGAGATGATGTTTGAGAAACAGAAACAGATAGGTGACAACAAGAGCCCTTCCCCTTCAAATGCACCTTCTGCTGCAGTGTTGGACACGACGCCACCTTCTTCTCCGGTGGACAATTTAAAAACTTCAAAAGATGAACGGGATAAATCAGGATGTAACGCTGACATTCCTAAAGACATACCTGGAGAAAGTTCCCATGATGTAAGCAGAAAGCAAATGGCAGATGAAGCTGAAGTTACCAATGAACATGAGAGGGTAGATGATCAGTTTTCTGACGTGCCTCCAACAAAACGGGCGAAGATTCAATGA
Protein sequence

>MsaT040757.1

MPSSSSPVLPSLMESNYMKPPDSFHRSPVRGLTANPASLQATSSYPIRSVRPVFSTNDECPGGVPFPPVSQHDRQYQDSPFASQALGDNVSSEIHSATFTSHLQENDDISWGPDPLPDILGFPEIFSVQHDQTMSKTDFGEWVEQLMTSDDSVNPNWSQLLGDDNVVEPTQQAMQVSQKQHTPSGEVNNLCNPASASASTASQTKPRMRWSPELHEAFVEAVNQLGGSEKATPKGVLNLMNVEGLTIYHVKSHLQKYRTARYKPESPEETSEKKMSSIEEMKSLDLKTSKGITEALRLQMELQKRLHEQLEIQRKLQIQIENQGKHLEMMFEKQKQIGDNKSPSPSNAPSAAVLDTTPPSSPVDNLKTSKDERDKSGCNADIPKDIPGESSHDVSRKQMADEAEVTNEHERVDDQFSDVPPTKRAKIQ*