AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040758

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040758 MtrunA17_Chr7g0274481 98.699 615 8 0 1 615 1 615 0.0 1239
MsaG040758 MtrunA17_Chr5g0397691 94.745 609 29 2 1 609 1 606 0.0 1082
MsaG040758 MtrunA17_Chr7g0238631 40.594 101 57 3 6 104 61 160 1.26e-14 77.0
MsaG040758 MtrunA17_Chr7g0238631 32.558 129 75 3 8 135 114 231 2.83e-11 66.2
MsaG040758 MtrunA17_Chr8g0362231 35.789 95 60 1 9 102 24 118 1.30e-13 73.2
MsaG040758 MtrunA17_Chr5g0416341 35.514 107 64 2 9 114 28 130 5.00e-13 71.6
MsaG040758 MtrunA17_Chr3g0139671 38.202 89 53 2 4 91 83 170 2.31e-12 70.5
MsaG040758 MtrunA17_Chr3g0139671 36.364 99 61 2 8 104 34 132 2.59e-12 70.1
MsaG040758 MtrunA17_Chr1g0157731 35.644 101 63 2 8 106 40 140 2.44e-12 70.5
MsaG040758 MtrunA17_Chr1g0157731 34.118 85 55 1 8 91 92 176 7.80e-11 65.5
MsaG040758 MtrunA17_Chr6g0483051 37.500 96 58 2 10 104 72 166 8.96e-12 68.2
MsaG040758 MtrunA17_Chr6g0460671 32.990 97 64 1 8 103 100 196 1.70e-11 66.2
MsaG040758 MtrunA17_Chr6g0480581 32.673 101 67 1 4 103 1 101 2.22e-11 64.3
MsaG040758 MtrunA17_Chr5g0397841 39.759 83 49 1 10 91 113 195 3.25e-11 65.9
MsaG040758 MtrunA17_Chr5g0417621 32.990 97 64 1 8 103 135 231 9.40e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040758 AT1G09770.1 84.262 610 93 2 1 609 1 608 0.0 1003
MsaG040758 AT5G11510.3 38.095 105 63 2 8 110 30 134 4.99e-14 76.3
MsaG040758 AT5G11510.1 38.095 105 63 2 8 110 30 134 4.99e-14 76.3
MsaG040758 AT5G11510.4 38.095 105 63 2 8 110 30 134 4.99e-14 76.3
MsaG040758 AT3G18100.1 36.458 96 60 1 8 102 547 642 5.96e-14 75.9
MsaG040758 AT5G02320.1 39.252 107 62 3 6 110 75 180 5.97e-14 75.5
MsaG040758 AT5G02320.1 36.471 85 53 1 8 91 128 212 7.74e-11 65.5
MsaG040758 AT5G02320.2 39.252 107 62 3 6 110 75 180 5.97e-14 75.5
MsaG040758 AT5G02320.2 36.471 85 53 1 8 91 128 212 7.74e-11 65.5
MsaG040758 AT5G02320.3 39.252 107 62 3 6 110 15 120 6.70e-14 75.1
MsaG040758 AT5G11510.2 38.095 105 63 2 8 110 30 134 1.03e-13 75.1
MsaG040758 AT3G18100.3 36.458 96 60 1 8 102 499 594 1.06e-13 75.1
MsaG040758 AT5G11510.5 38.095 105 63 2 8 110 30 134 1.16e-13 74.7
MsaG040758 AT3G18100.4 36.458 96 60 1 8 102 386 481 1.23e-13 74.7
MsaG040758 AT3G18100.2 36.458 96 60 1 8 102 334 429 1.90e-13 73.9
MsaG040758 AT4G33450.1 35.052 97 62 1 8 103 20 116 1.26e-12 68.6
MsaG040758 AT2G02820.2 31.933 119 66 2 9 112 32 150 2.19e-12 70.1
MsaG040758 AT2G02820.1 31.933 119 66 2 9 112 32 150 2.66e-12 69.7
MsaG040758 AT3G09370.2 38.318 107 63 3 6 110 83 188 2.88e-12 69.7
MsaG040758 AT3G09370.1 38.318 107 63 3 6 110 78 183 2.92e-12 69.7
MsaG040758 AT1G14350.2 34.375 96 62 1 9 103 27 122 3.10e-12 69.3
MsaG040758 AT1G14350.1 34.375 96 62 1 9 103 27 122 3.10e-12 69.3
MsaG040758 AT1G17950.1 32.353 102 68 1 3 103 1 102 3.67e-12 67.4
MsaG040758 AT3G09370.3 38.318 107 63 3 6 110 91 196 4.38e-12 69.3
MsaG040758 AT3G27920.1 33.645 107 69 2 6 110 15 121 5.12e-12 66.6
MsaG040758 AT5G40360.1 37.805 82 51 0 4 85 155 236 7.29e-12 67.8
MsaG040758 AT2G02820.3 31.933 119 66 2 9 112 32 150 7.46e-12 67.4
MsaG040758 AT5G14750.1 36.264 91 56 2 6 94 17 107 7.81e-12 65.5
MsaG040758 AT4G18770.1 40.000 85 50 1 8 91 218 302 1.66e-11 67.0
MsaG040758 AT4G32730.5 34.343 99 63 2 8 104 36 134 1.79e-11 67.8
MsaG040758 AT4G32730.4 34.343 99 63 2 8 104 36 134 1.79e-11 67.8
MsaG040758 AT4G32730.3 34.343 99 63 2 8 104 36 134 1.79e-11 67.8
MsaG040758 AT4G32730.2 34.343 99 63 2 8 104 36 134 1.79e-11 67.8
MsaG040758 AT4G32730.1 34.343 99 63 2 8 104 36 134 2.96e-11 67.0
MsaG040758 AT5G17800.1 34.021 97 63 1 8 103 94 190 3.13e-11 65.5
MsaG040758 AT4G00540.2 38.596 114 59 5 6 109 46 158 4.07e-11 65.9
MsaG040758 AT4G00540.1 38.596 114 59 5 6 109 44 156 5.71e-11 65.5
MsaG040758 AT1G69560.1 33.000 100 66 1 5 103 105 204 7.46e-11 64.3
MsaG040758 AT1G69560.2 33.000 100 66 1 5 103 121 220 7.94e-11 64.3
MsaG040758 AT5G67300.1 31.092 119 79 2 8 123 7 125 8.03e-11 63.9
MsaG040758 AT5G11050.1 38.636 88 53 1 5 91 103 190 8.39e-11 65.1

Find 189 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTCTTTCTAACTGTTTC+TGG 0.008572 7:-103598151 None:intergenic
TGCCGGCGTCATACCACTTC+TGG 0.183947 7:-103598566 None:intergenic
ATTTCATAACAGCAGCTTTC+AGG 0.225007 7:-103597346 None:intergenic
GGAGTAAGGCCTGCACTTCC+AGG 0.240853 7:-103598544 None:intergenic
TTGGGTGTCTGTGGATAATT+AGG 0.241982 7:-103598304 None:intergenic
CATGATGGAAGCTGAAAATT+TGG 0.248037 7:+103598387 MsaT040758.1:CDS
TGGCATCTCTGGAGCTTATT+AGG 0.252115 7:+103598935 MsaT040758.1:CDS
GGTCGCGCAGGCTTTGATTC+AGG 0.281831 7:-103597686 None:intergenic
GTTCTCTGAGGAGTTCTTAA+AGG 0.283026 7:-103598340 None:intergenic
AAAGCTGCTGTTATGAAATA+TGG 0.287568 7:+103597350 MsaT040758.1:CDS
GAATTGGTTGCATCACTATC+TGG 0.302904 7:-103598765 None:intergenic
TTCTGGGTCATTCAGCTTGT+TGG 0.304170 7:-103598134 None:intergenic
CGGGGTCACTCCTAAGAAAA+AGG 0.305366 7:+103598480 MsaT040758.1:CDS
AAAGGAGCTTCTAGCCTTAC+TGG 0.309592 7:+103599038 MsaT040758.1:CDS
AGTCCACTCAGTCTTCTTAA+TGG 0.314486 7:-103597462 None:intergenic
ATCTCTTTCTAACTGTTTCT+GGG 0.335857 7:-103598150 None:intergenic
TAGGCCTCCTCTGGCATCTC+TGG 0.337343 7:+103598924 MsaT040758.1:CDS
TCTTCAGCATCTTCTTGAAT+TGG 0.338010 7:-103598781 None:intergenic
ATCTCGTCGGCTTGCTCAAT+AGG 0.342238 7:-103599009 None:intergenic
GAAGGAGTCAGCAGTGGGTT+TGG 0.354854 7:-103598514 None:intergenic
CTCCGAAGGGAACTCCTCTC+AGG 0.358383 7:+103598614 MsaT040758.1:CDS
TCAAGTTCTTCAATAGTAGT+AGG 0.370329 7:-103598010 None:intergenic
GCTTGAGGAGATAGCAAATA+AGG 0.373696 7:+103599083 MsaT040758.1:CDS
CCTTGACACCGCTTGTGTTA+AGG 0.376504 7:+103597601 MsaT040758.1:CDS
AGAAGAAGAGGCACGGCAGC+AGG 0.379807 7:+103598858 MsaT040758.1:CDS
AAAGGAGTCTTGGCTTGGTT+GGG 0.383959 7:-103598322 None:intergenic
AGGATCAAGCAGGCAGCAAA+TGG 0.385258 7:+103599116 MsaT040758.1:CDS
TCTCGTCGGCTTGCTCAATA+GGG 0.387795 7:-103599008 None:intergenic
TCAGATTTCCGATCAAGAAT+TGG 0.388348 7:+103598195 MsaT040758.1:CDS
AGGCTGCTGGGATTGATATA+AGG 0.394781 7:+103597867 MsaT040758.1:CDS
GCAGGCTTTGATTCAGGATT+CGG 0.400720 7:-103597680 None:intergenic
AAAATGAGGATTATGATAAA+GGG 0.404294 7:+103597293 None:intergenic
TTATGATAAAGGGAGGTGTT+TGG 0.408987 7:+103597303 MsaT040758.1:CDS
TCTGCAACCCCTGGAAGTGC+AGG 0.411721 7:+103598535 MsaT040758.1:CDS
GCTACATATCAATGAAGATA+TGG 0.420115 7:+103598642 MsaT040758.1:CDS
TAAGGCAGCGGAGGAGGAAA+AGG 0.424744 7:+103597885 MsaT040758.1:CDS
TTCTTAAAGGAGTCTTGGCT+TGG 0.428361 7:-103598327 None:intergenic
GCCGGCGTCATACCACTTCT+GGG 0.428742 7:-103598565 None:intergenic
TTGGATGAAATTGCCAAGTT+GGG 0.429250 7:+103598214 MsaT040758.1:CDS
TGCATCCTTCAGATTTCTCC+GGG 0.432408 7:+103598461 MsaT040758.1:CDS
GCGACCTGATCCTGTTGATA+TGG 0.436108 7:+103597703 MsaT040758.1:CDS
AGCTTTCAGGATTTCATCCT+CGG 0.436489 7:-103597333 None:intergenic
GTTTGAGAAGAGGCCTCCTC+CGG 0.436599 7:+103597937 MsaT040758.1:CDS
TCCTCTTCAATCTTCTCCTC+AGG 0.438758 7:-103598805 None:intergenic
AAGAACTTGAGGGGAAAAGA+AGG 0.442866 7:+103598023 MsaT040758.1:CDS
TTGCATCCTTCAGATTTCTC+CGG 0.443045 7:+103598460 MsaT040758.1:CDS
TAAAGGAGTCTTGGCTTGGT+TGG 0.443688 7:-103598323 None:intergenic
ATTGGATGAAATTGCCAAGT+TGG 0.444138 7:+103598213 MsaT040758.1:CDS
CTCGCTGCCCACAAGGTCAC+TGG 0.444838 7:-103598242 None:intergenic
GCCTAGGAAGTTCCCTTTGA+AGG 0.445432 7:-103598906 None:intergenic
AGAACCATTGCTCCCATTGT+TGG 0.447103 7:+103597539 MsaT040758.1:CDS
TCATCCTCATCCATATCAAC+AGG 0.447260 7:-103597713 None:intergenic
AGCCAACCGAGCACGTGCTT+CGG 0.447500 7:-103597750 None:intergenic
AGAGATGCCAGAGGAGGCCT+AGG 0.450878 7:-103598922 None:intergenic
TCCTGATAATCTTCTATGAC+AGG 0.451422 7:-103599149 None:intergenic
AAGAGAGAACTGAAGGCTGC+TGG 0.453605 7:+103597854 MsaT040758.1:CDS
AGGAGTTCTTAAAGGAGTCT+TGG 0.456240 7:-103598332 None:intergenic
TGCAGATTTGCGAACGAGTA+GGG 0.456491 7:-103597399 None:intergenic
TCATACCACTTCTGGGAGTA+AGG 0.456938 7:-103598558 None:intergenic
ATAGCATCTCCTTTACTAGT+TGG 0.464658 7:-103598364 None:intergenic
TCTTCTATGACAGGGATAGT+AGG 0.466162 7:-103599140 None:intergenic
CAGAAATTCCGTTTGAGAAG+AGG 0.469889 7:+103597927 MsaT040758.1:CDS
GAGTCTCAGACACCACTGTT+AGG 0.471291 7:+103598421 MsaT040758.1:CDS
CTGAAAATTTGGCCAGGCTG+AGG 0.471566 7:+103598398 MsaT040758.1:CDS
TTGATATAAGGCAGCGGAGG+AGG 0.474103 7:+103597879 MsaT040758.1:CDS
TTCCGAAGCACGTGCTCGGT+TGG 0.478007 7:+103597748 MsaT040758.1:CDS
TGATGTTGCTGATGAGGATA+GGG 0.479449 7:+103597967 MsaT040758.1:CDS
AATTTCATCCAATTCTTGAT+CGG 0.479560 7:-103598203 None:intergenic
GGAACTTCCTAGGCCTCCTC+TGG 0.482790 7:+103598915 MsaT040758.1:CDS
TGTCTGAGACTCCCTCAGCC+TGG 0.484905 7:-103598410 None:intergenic
TCCTTCAAAGGGAACTTCCT+AGG 0.489332 7:+103598905 MsaT040758.1:CDS
TGACCCTAGGAAATTGCGTC+CGG 0.489894 7:+103597646 MsaT040758.1:CDS
TTGGCATGCAGAATAGCAGC+TGG 0.489948 7:-103598115 None:intergenic
GAGTAAGGCCTGCACTTCCA+GGG 0.490905 7:-103598543 None:intergenic
GCAAATAAGGAGAAGAAGAA+AGG 0.491928 7:+103599096 MsaT040758.1:CDS
CTCCTTTCTTATCATCTCGT+CGG 0.493278 7:-103599022 None:intergenic
TGCTTTCCGAAGCACGTGCT+CGG 0.495069 7:+103597744 MsaT040758.1:CDS
AATCTGAAGGATGCAACTCC+GGG 0.495306 7:-103598453 None:intergenic
ACGAGTAGCACTGCTGCCTT+CGG 0.495690 7:-103598272 None:intergenic
GTCTCCGTCAGCTCTTATCA+AGG 0.499862 7:-103598962 None:intergenic
AAATCTGAAGGATGCAACTC+CGG 0.500871 7:-103598454 None:intergenic
AACGATCAAAAGTCCTTCAA+AGG 0.501321 7:+103598893 MsaT040758.1:CDS
AGAGGATATGTCTGATAGAA+TGG 0.507303 7:+103598822 MsaT040758.1:CDS
TCCCTGAGAGGAGTTCCCTT+CGG 0.510495 7:-103598616 None:intergenic
TGATAGAATGGCTAGAGAAA+AGG 0.511992 7:+103598834 MsaT040758.1:CDS
CGAAGGAGTACGACCAACAA+TGG 0.518743 7:-103597552 None:intergenic
TGGGTGTCTGTGGATAATTA+GGG 0.519886 7:-103598303 None:intergenic
CAGAGAACACCAACTAGTAA+AGG 0.520398 7:+103598355 MsaT040758.1:CDS
AGAGAGAACTGAAGGCTGCT+GGG 0.523302 7:+103597855 MsaT040758.1:CDS
TCATCCATATCAACAGGATC+AGG 0.524361 7:-103597707 None:intergenic
TTGATGTTGCTGATGAGGAT+AGG 0.524642 7:+103597966 MsaT040758.1:CDS
TCGGCTTGCTCAATAGGGGT+TGG 0.524733 7:-103599003 None:intergenic
TGAAAATTTGGCCAGGCTGA+GGG 0.525084 7:+103598399 MsaT040758.1:CDS
AGCATTGTCGTGCTCCAGTA+AGG 0.526571 7:-103599052 None:intergenic
CTGACTCCTTCTGCAACCCC+TGG 0.529271 7:+103598526 MsaT040758.1:CDS
GCTTGCTCAATAGGGGTTGG+TGG 0.531592 7:-103599000 None:intergenic
CACTCAGTCTTCTTAATGGA+AGG 0.532258 7:-103597458 None:intergenic
GGAGGAGGCCTCTTCTCAAA+CGG 0.537221 7:-103597935 None:intergenic
TTGGCTTGGTTGGGTGTCTG+TGG 0.541672 7:-103598313 None:intergenic
GACCCTAGGAAATTGCGTCC+GGG 0.542095 7:+103597647 MsaT040758.1:CDS
CAAGTTCTTCAATAGTAGTA+GGG 0.542935 7:-103598009 None:intergenic
TACTACTATTGAAGAACTTG+AGG 0.549669 7:+103598012 MsaT040758.1:CDS
GGATCAAGCAGGCAGCAAAT+GGG 0.550305 7:+103599117 MsaT040758.1:CDS
ACTACTATTGAAGAACTTGA+GGG 0.551426 7:+103598013 MsaT040758.1:CDS
AGCCGACGAGATGATAAGAA+AGG 0.552168 7:+103599020 MsaT040758.1:CDS
GAAAAGAAGGATTGATGTTG+AGG 0.552508 7:+103598036 MsaT040758.1:CDS
CCTTAACACAAGCGGTGTCA+AGG 0.552579 7:-103597601 None:intergenic
TGATCGGAAATCTGAGGCGG+TGG 0.554468 7:-103598187 None:intergenic
GGTTATGCCAGTGACCTTGT+GGG 0.556103 7:+103598235 MsaT040758.1:CDS
TGAGAAAGCAAGACGTTGCA+AGG 0.556741 7:+103598065 MsaT040758.1:CDS
GGGTTATGCCAGTGACCTTG+TGG 0.560009 7:+103598234 MsaT040758.1:CDS
TTGCAGATTTGCGAACGAGT+AGG 0.561949 7:-103597400 None:intergenic
GCACTTCCAGGGGTTGCAGA+AGG 0.564971 7:-103598532 None:intergenic
AAGTGGTATGACGCCGGCAA+GGG 0.565169 7:+103598570 MsaT040758.1:CDS
ACGATCAAAAGTCCTTCAAA+GGG 0.567785 7:+103598894 MsaT040758.1:CDS
TTACTAGTTGGTGTTCTCTG+AGG 0.567840 7:-103598352 None:intergenic
CAAAACAGTGTAAAGCTCGT+TGG 0.569337 7:+103597420 MsaT040758.1:CDS
AGAAGAAGAAAGGATCAAGC+AGG 0.570232 7:+103599106 MsaT040758.1:CDS
GGTATGACGCCGGCAAGGGA+TGG 0.571829 7:+103598574 MsaT040758.1:CDS
TAAAGGAGATGCTATCATGA+TGG 0.573963 7:+103598372 MsaT040758.1:CDS
TGAGTTCCTCGCTGCCCACA+AGG 0.576016 7:-103598249 None:intergenic
ACCCTAGGAAATTGCGTCCG+GGG 0.576243 7:+103597648 MsaT040758.1:CDS
GAAGGAGTACGACCAACAAT+GGG 0.576764 7:-103597551 None:intergenic
TAGAGAAAAGGCAGAAGAAG+AGG 0.579560 7:+103598846 MsaT040758.1:CDS
ATGAACCTGGTGATGACCCT+AGG 0.580871 7:+103597633 MsaT040758.1:CDS
TCCGAAGGGAACTCCTCTCA+GGG 0.580985 7:+103598615 MsaT040758.1:CDS
ACATCAAAATAACCCGGAGG+AGG 0.581361 7:-103597950 None:intergenic
TTGCAGAAGGAGTCAGCAGT+GGG 0.581838 7:-103598519 None:intergenic
TTATTTGCTATCTCCTCAAG+CGG 0.583374 7:-103599081 None:intergenic
GTCACTGGCATAACCCAACT+TGG 0.585958 7:-103598227 None:intergenic
AGTAAGGCCTGCACTTCCAG+GGG 0.586407 7:-103598542 None:intergenic
TGATATGGATGAGGATGAGA+AGG 0.587713 7:+103597718 MsaT040758.1:CDS
AACTCCTTGATAAGAGCTGA+CGG 0.589304 7:+103598958 MsaT040758.1:CDS
CCTGATAATCTTCTATGACA+GGG 0.589597 7:-103599148 None:intergenic
GTAAAGCTCGTTGGTATGAG+TGG 0.590546 7:+103597429 MsaT040758.1:CDS
ATAGTGCTAAGCTCGAGCTA+CGG 0.590781 7:+103598674 MsaT040758.1:CDS
GACTGAGTGGACTCGAGAAG+AGG 0.592125 7:+103597472 MsaT040758.1:CDS
TTTGAGAAGAGGCCTCCTCC+GGG 0.592447 7:+103597938 MsaT040758.1:CDS
TGGCATGCAGAATAGCAGCT+GGG 0.592750 7:-103598114 None:intergenic
GAAGTGGTATGACGCCGGCA+AGG 0.595106 7:+103598569 MsaT040758.1:CDS
TGCTGATGAGGATAGGGAGG+TGG 0.595277 7:+103597973 MsaT040758.1:CDS
AGCTCCAGAGATGCCAGAGG+AGG 0.595601 7:-103598928 None:intergenic
AAAAGGCAGAAGAAGAGGCA+CGG 0.599691 7:+103598851 MsaT040758.1:CDS
GCAATGGTTCTCCACTGAGT+AGG 0.605858 7:-103597527 None:intergenic
TCAACAGGATCAGGTCGCGC+AGG 0.606256 7:-103597698 None:intergenic
TGGAAGCTGAAAATTTGGCC+AGG 0.607474 7:+103598392 MsaT040758.1:CDS
TCTCAGACACCACTGTTAGG+AGG 0.609284 7:+103598424 MsaT040758.1:CDS
GCTCGGTTGGCTAACACGAA+AGG 0.609461 7:+103597761 MsaT040758.1:CDS
AGCGGAGGAGGAAAAGGAGA+GGG 0.609580 7:+103597891 MsaT040758.1:CDS
AGAAACAGCTTGAGGAAGCG+AGG 0.613924 7:+103597810 MsaT040758.1:CDS
GTTGCAGAAGGAGTCAGCAG+TGG 0.614178 7:-103598520 None:intergenic
ATGAGGATTATGATAAAGGG+AGG 0.615783 7:+103597296 MsaT040758.1:CDS
GTGACCCCGGAGAAATCTGA+AGG 0.618504 7:-103598466 None:intergenic
CCCTAGGAAATTGCGTCCGG+GGG 0.618511 7:+103597649 MsaT040758.1:CDS
TGATCCTGTTGATATGGATG+AGG 0.618540 7:+103597709 MsaT040758.1:CDS
CAATGCTAAGTATCCGCTTG+AGG 0.619069 7:+103599068 MsaT040758.1:CDS
AATTTCCTAGGGTCATCACC+AGG 0.619740 7:-103597638 None:intergenic
CAGCGGAGGAGGAAAAGGAG+AGG 0.619802 7:+103597890 MsaT040758.1:CDS
CTGGGATTGATATAAGGCAG+CGG 0.621106 7:+103597873 MsaT040758.1:CDS
GCAGGCCTTACTCCCAGAAG+TGG 0.624004 7:+103598553 MsaT040758.1:CDS
ACGACCAACAATGGGAGCAA+TGG 0.628407 7:-103597543 None:intergenic
AGAAGATGCTGAAGAGCCTG+AGG 0.629986 7:+103598789 MsaT040758.1:CDS
GGATTCGGATCAATCTCCCC+CGG 0.632404 7:-103597665 None:intergenic
CTCGTCGGCTTGCTCAATAG+GGG 0.633774 7:-103599007 None:intergenic
AACAGCTTGAGGAAGCGAGG+AGG 0.635219 7:+103597813 MsaT040758.1:CDS
GTTATCATCCTTAACACAAG+CGG 0.635250 7:-103597609 None:intergenic
GGCATGCAGAATAGCAGCTG+GGG 0.637586 7:-103598113 None:intergenic
GCCTGAGGAGAAGATTGAAG+AGG 0.638365 7:+103598804 MsaT040758.1:CDS
GGCAGCGAGGAACTCACCGA+AGG 0.640181 7:+103598256 MsaT040758.1:CDS
TCCCAGAAGTGGTATGACGC+CGG 0.645263 7:+103598564 MsaT040758.1:CDS
TGTTGCTGATGAGGATAGGG+AGG 0.645550 7:+103597970 MsaT040758.1:CDS
CTACTATTGAAGAACTTGAG+GGG 0.649822 7:+103598014 MsaT040758.1:CDS
TCAATAGTAGTAGGGAACGC+AGG 0.663373 7:-103598001 None:intergenic
AGCTAGGGAGAAACAGCTTG+AGG 0.665565 7:+103597802 MsaT040758.1:CDS
AATTCTTGATCGGAAATCTG+AGG 0.665716 7:-103598193 None:intergenic
AAGGATGATAACTATGAACC+TGG 0.668836 7:+103597620 MsaT040758.1:CDS
TAGCGTTCAAGACACTGCGA+AGG 0.670461 7:-103597569 None:intergenic
TCAGCAACATCAAAATAACC+CGG 0.679101 7:-103597956 None:intergenic
CTTCCATTAAGAAGACTGAG+TGG 0.680364 7:+103597459 MsaT040758.1:CDS
CAGTGACCTTGTGGGCAGCG+AGG 0.681072 7:+103598243 MsaT040758.1:CDS
ACTGTTAGGAGGAGAAAACC+CGG 0.686147 7:+103598435 MsaT040758.1:CDS
ATATGTAGCTCATCCCTGAG+AGG 0.688581 7:-103598628 None:intergenic
CTAAGCTTATGCCTACTCAG+TGG 0.690760 7:+103597516 MsaT040758.1:CDS
TCTTGATCGGAAATCTGAGG+CGG 0.695426 7:-103598190 None:intergenic
GGATTGATATAAGGCAGCGG+AGG 0.699913 7:+103597876 MsaT040758.1:CDS
ATAAGCTCCAGAGATGCCAG+AGG 0.701530 7:-103598931 None:intergenic
CAAGGAATAAAATTGCAGAG+AGG 0.745082 7:+103598083 MsaT040758.1:CDS
GCATCCTTCAGATTTCTCCG+GGG 0.756401 7:+103598462 MsaT040758.1:CDS
GCAACATCAAAATAACCCGG+AGG 0.761555 7:-103597953 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 103597296 103599158 103597296 ID=MsaG040758
Chr7 mRNA 103597296 103599158 103597296 ID=MsaT040758.1;Parent=MsaG040758
Chr7 exon 103597296 103599158 103597296 ID=MsaT040758.1.exon1;Parent=MsaT040758.1
Chr7 CDS 103597296 103599158 103597296 ID=cds.MsaT040758.1;Parent=MsaT040758.1
Gene Sequence

>MsaT040758.1

ATGAGGATTATGATAAAGGGAGGTGTTTGGAAAAACACCGAGGATGAAATCCTGAAAGCTGCTGTTATGAAATATGGTAAAAATCAGTGGGCTCGTATCTCGTCCCTACTCGTTCGCAAATCTGCAAAACAGTGTAAAGCTCGTTGGTATGAGTGGTTAGATCCTTCCATTAAGAAGACTGAGTGGACTCGAGAAGAGGATGAGAAGCTACTTCATCTTGCTAAGCTTATGCCTACTCAGTGGAGAACCATTGCTCCCATTGTTGGTCGTACTCCTTCGCAGTGTCTTGAACGCTATGAGAAACTCCTTGACACCGCTTGTGTTAAGGATGATAACTATGAACCTGGTGATGACCCTAGGAAATTGCGTCCGGGGGAGATTGATCCGAATCCTGAATCAAAGCCTGCGCGACCTGATCCTGTTGATATGGATGAGGATGAGAAGGAGATGCTTTCCGAAGCACGTGCTCGGTTGGCTAACACGAAAGGTAAAAAAGCAAAAAGGAAAGCTAGGGAGAAACAGCTTGAGGAAGCGAGGAGGCTCGCTTCTTTACAGAAAAAGAGAGAACTGAAGGCTGCTGGGATTGATATAAGGCAGCGGAGGAGGAAAAGGAGAGGGATTGATTATAATGCAGAAATTCCGTTTGAGAAGAGGCCTCCTCCGGGTTATTTTGATGTTGCTGATGAGGATAGGGAGGTGGAGCAGCCTGCGTTCCCTACTACTATTGAAGAACTTGAGGGGAAAAGAAGGATTGATGTTGAGGCACAGTTGAGAAAGCAAGACGTTGCAAGGAATAAAATTGCAGAGAGGCAAGATGCCCCAGCTGCTATTCTGCATGCCAACAAGCTGAATGACCCAGAAACAGTTAGAAAGAGATCAAAACTGATGCTTCCACCGCCTCAGATTTCCGATCAAGAATTGGATGAAATTGCCAAGTTGGGTTATGCCAGTGACCTTGTGGGCAGCGAGGAACTCACCGAAGGCAGCAGTGCTACTCGTGCTCTTCTCCCTAATTATCCACAGACACCCAACCAAGCCAAGACTCCTTTAAGAACTCCTCAGAGAACACCAACTAGTAAAGGAGATGCTATCATGATGGAAGCTGAAAATTTGGCCAGGCTGAGGGAGTCTCAGACACCACTGTTAGGAGGAGAAAACCCGGAGTTGCATCCTTCAGATTTCTCCGGGGTCACTCCTAAGAAAAAGGAAATTCACACTCCAAACCCACTGCTGACTCCTTCTGCAACCCCTGGAAGTGCAGGCCTTACTCCCAGAAGTGGTATGACGCCGGCAAGGGATGGTTTTTCTTTTGGCATGACTCCGAAGGGAACTCCTCTCAGGGATGAGCTACATATCAATGAAGATATGGAGATGCGTGATAGTGCTAAGCTCGAGCTACGGAGACAAGCTGATATGAAAAAAAGTCTGCGCTCTGGTTTAAGCAGCCTTCCACAGCCTAAAAATGAGTACCAGATAGTGATGCAACCAATTCAAGAAGATGCTGAAGAGCCTGAGGAGAAGATTGAAGAGGATATGTCTGATAGAATGGCTAGAGAAAAGGCAGAAGAAGAGGCACGGCAGCAGGCATTACTTAGAAAACGATCAAAAGTCCTTCAAAGGGAACTTCCTAGGCCTCCTCTGGCATCTCTGGAGCTTATTAGGAACTCCTTGATAAGAGCTGACGGAGACAAAAGTTCATTTGTTCCACCAACCCCTATTGAGCAAGCCGACGAGATGATAAGAAAGGAGCTTCTAGCCTTACTGGAGCACGACAATGCTAAGTATCCGCTTGAGGAGATAGCAAATAAGGAGAAGAAGAAAGGATCAAGCAGGCAGCAAATGGGCCTACTATCCCTGTCATAG
Protein sequence

>MsaT040758.1

MRIMIKGGVWKNTEDEILKAAVMKYGKNQWARISSLLVRKSAKQCKARWYEWLDPSIKKTEWTREEDEKLLHLAKLMPTQWRTIAPIVGRTPSQCLERYEKLLDTACVKDDNYEPGDDPRKLRPGEIDPNPESKPARPDPVDMDEDEKEMLSEARARLANTKGKKAKRKAREKQLEEARRLASLQKKRELKAAGIDIRQRRRKRRGIDYNAEIPFEKRPPPGYFDVADEDREVEQPAFPTTIEELEGKRRIDVEAQLRKQDVARNKIAERQDAPAAILHANKLNDPETVRKRSKLMLPPPQISDQELDEIAKLGYASDLVGSEELTEGSSATRALLPNYPQTPNQAKTPLRTPQRTPTSKGDAIMMEAENLARLRESQTPLLGGENPELHPSDFSGVTPKKKEIHTPNPLLTPSATPGSAGLTPRSGMTPARDGFSFGMTPKGTPLRDELHINEDMEMRDSAKLELRRQADMKKSLRSGLSSLPQPKNEYQIVMQPIQEDAEEPEEKIEEDMSDRMAREKAEEEARQQALLRKRSKVLQRELPRPPLASLELIRNSLIRADGDKSSFVPPTPIEQADEMIRKELLALLEHDNAKYPLEEIANKEKKKGSSRQQMGLLSLS*