AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040886

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040886 MtrunA17_Chr7g0275531 91.642 335 19 2 1 333 1 328 0.0 619
MsaG040886 MtrunA17_Chr8g0347251 44.837 368 141 15 1 329 1 345 2.43e-78 243
MsaG040886 MtrunA17_Chr8g0387981 36.585 369 189 12 1 332 1 361 1.51e-55 184
MsaG040886 MtrunA17_Chr3g0126391 53.125 192 67 9 12 185 7 193 1.17e-53 179
MsaG040886 MtrunA17_Chr7g0244981 33.500 200 108 5 9 198 11 195 3.74e-26 105
MsaG040886 MtrunA17_Chr7g0244971 32.275 189 97 5 9 184 12 182 3.73e-25 102
MsaG040886 MtrunA17_Chr8g0334541 36.364 176 85 5 15 184 8 162 1.60e-24 100
MsaG040886 MtrunA17_Chr2g0315371 30.622 209 109 6 11 195 15 211 6.66e-21 91.3
MsaG040886 MtrunA17_Chr8g0350621 30.556 180 96 7 15 184 10 170 1.82e-20 89.4
MsaG040886 MtrunA17_Chr5g0428351 31.892 185 101 6 7 189 87 248 1.47e-19 87.8
MsaG040886 MtrunA17_Chr3g0128671 30.570 193 103 5 18 196 23 198 1.75e-19 87.4
MsaG040886 MtrunA17_Chr2g0315381 45.349 86 37 2 100 184 127 203 6.32e-19 85.9
MsaG040886 MtrunA17_Chr2g0315361 45.349 86 38 2 99 184 131 207 1.00e-17 82.4
MsaG040886 MtrunA17_Chr1g0147881 43.529 85 44 2 98 182 237 317 2.75e-16 78.6
MsaG040886 MtrunA17_Chr7g0270051 40.870 115 63 3 78 189 3 115 4.16e-16 77.0
MsaG040886 MtrunA17_Chr7g0224801 55.224 67 25 2 124 190 92 153 1.16e-15 73.9
MsaG040886 MtrunA17_Chr7g0270191 40.594 101 56 2 88 187 107 204 1.45e-15 75.9
MsaG040886 MtrunA17_Chr8g0348311 38.679 106 59 3 82 187 114 213 3.19e-15 75.5
MsaG040886 MtrunA17_Chr4g0071521 32.624 141 72 3 42 182 183 300 3.43e-15 75.1
MsaG040886 MtrunA17_Chr8g0387501 47.297 74 33 3 121 194 125 192 4.25e-15 74.3
MsaG040886 MtrunA17_Chr8g0379461 36.885 122 65 3 99 215 45 159 7.03e-15 73.2
MsaG040886 MtrunA17_Chr7g0248691 51.515 66 26 2 126 191 155 214 2.93e-14 72.4
MsaG040886 MtrunA17_Chr5g0401821 54.386 57 24 1 126 182 200 254 3.38e-14 71.6
MsaG040886 MtrunA17_Chr2g0293481 41.748 103 52 4 126 222 268 368 4.40e-14 72.8
MsaG040886 MtrunA17_Chr2g0319961 43.750 96 48 3 99 190 50 143 5.86e-14 70.9
MsaG040886 MtrunA17_Chr5g0418781 52.381 63 26 2 124 186 123 181 7.27e-14 70.9
MsaG040886 MtrunA17_Chr2g0284321 46.479 71 32 2 126 196 36 100 8.55e-14 69.7
MsaG040886 MtrunA17_Chr1g0184691 52.632 57 25 1 126 182 294 348 2.04e-13 70.5
MsaG040886 MtrunA17_Chr8g0386241 46.269 67 31 2 124 190 102 163 2.51e-13 67.4
MsaG040886 MtrunA17_Chr4g0053281 50.877 57 26 1 126 182 267 321 5.25e-13 68.9
MsaG040886 MtrunA17_Chr4g0042831 44.118 68 35 1 125 192 145 209 5.50e-13 69.3
MsaG040886 MtrunA17_Chr4g0042831 41.772 79 43 1 112 190 265 340 1.82e-11 64.7
MsaG040886 MtrunA17_Chr1g0194481 50.000 64 27 2 124 187 157 215 5.50e-13 68.9
MsaG040886 MtrunA17_Chr4g0051881 47.143 70 32 2 124 193 177 241 5.93e-13 68.9
MsaG040886 MtrunA17_Chr5g0443131 49.153 59 27 1 124 182 156 211 6.01e-13 68.6
MsaG040886 MtrunA17_Chr3g0079691 45.714 70 32 3 125 194 280 343 6.08e-13 69.7
MsaG040886 MtrunA17_Chr3g0079691 33.663 101 59 2 124 219 494 591 5.33e-11 63.9
MsaG040886 MtrunA17_Chr7g0228941 52.632 57 25 1 126 182 53 107 6.38e-13 65.1
MsaG040886 MtrunA17_Chr3g0129361 44.444 72 37 1 112 183 131 199 9.40e-13 66.6
MsaG040886 MtrunA17_Chr2g0294231 48.438 64 30 1 124 187 202 262 1.05e-12 68.2
MsaG040886 MtrunA17_Chr8g0383351 50.000 60 27 1 124 183 159 215 1.10e-12 66.6
MsaG040886 MtrunA17_Chr7g0218081 46.875 64 30 2 125 188 228 287 1.25e-12 68.6
MsaG040886 MtrunA17_Chr7g0218081 44.776 67 34 1 124 190 409 472 3.17e-12 67.4
MsaG040886 MtrunA17_Chr5g0418191 46.269 67 33 1 124 190 319 382 1.32e-12 68.2
MsaG040886 MtrunA17_Chr5g0418191 35.652 115 65 4 125 239 151 256 4.51e-11 63.5
MsaG040886 MtrunA17_Chr7g0263531 31.667 120 78 2 126 245 190 305 1.49e-12 68.2
MsaG040886 MtrunA17_Chr7g0238921 49.275 69 28 2 114 182 117 178 1.87e-12 67.0
MsaG040886 MtrunA17_Chr5g0445311 44.776 67 34 1 124 190 363 426 2.37e-12 67.8
MsaG040886 MtrunA17_Chr5g0445311 45.833 72 33 3 123 194 198 263 3.98e-12 67.0
MsaG040886 MtrunA17_Chr5g0431811 43.284 67 38 0 125 191 672 738 2.57e-12 67.8
MsaG040886 MtrunA17_Chr1g0202461 44.928 69 32 2 126 194 262 324 2.82e-12 67.0
MsaG040886 MtrunA17_Chr3g0139021 52.632 57 25 1 126 182 315 369 3.13e-12 67.0
MsaG040886 MtrunA17_Chr3g0101131 47.143 70 31 3 125 194 243 306 3.16e-12 67.4
MsaG040886 MtrunA17_Chr3g0101131 44.776 67 34 1 124 190 414 477 5.25e-12 66.6
MsaG040886 MtrunA17_Chr8g0335811 45.070 71 36 1 112 182 141 208 4.03e-12 65.1
MsaG040886 MtrunA17_Chr4g0068541 45.312 64 32 1 124 187 207 267 8.69e-12 65.9
MsaG040886 MtrunA17_Chr7g0249311 44.615 65 32 2 125 189 179 239 9.17e-12 66.2
MsaG040886 MtrunA17_Chr7g0248721 43.902 82 40 3 124 205 140 215 1.12e-11 63.5
MsaG040886 MtrunA17_Chr2g0322391 48.485 66 32 1 124 189 348 411 2.97e-11 64.3
MsaG040886 MtrunA17_Chr5g0432161 44.776 67 34 1 124 190 420 483 3.11e-11 64.3
MsaG040886 MtrunA17_Chr1g0150801 38.824 85 45 3 107 189 82 161 6.85e-11 60.1
MsaG040886 MtrunA17_Chr3g0106491 43.284 67 35 1 124 190 364 427 9.09e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040886 AT4G11070.1 38.728 346 147 13 3 324 2 306 2.52e-51 172
MsaG040886 AT4G11070.2 37.423 326 147 11 3 324 2 274 9.61e-47 160
MsaG040886 AT5G24110.1 37.066 259 120 10 12 265 17 237 8.00e-43 150
MsaG040886 AT4G23810.1 42.478 226 85 7 11 195 13 234 8.79e-43 150
MsaG040886 AT2G46400.1 37.542 301 153 10 12 309 8 276 9.74e-41 144
MsaG040886 AT3G56400.1 32.995 197 98 5 3 184 2 179 9.72e-27 107
MsaG040886 AT2G40750.1 27.941 272 160 7 5 244 8 275 1.27e-23 100
MsaG040886 AT2G40740.1 42.202 109 61 1 99 205 145 253 2.70e-22 95.5
MsaG040886 AT2G40740.3 42.202 109 61 1 99 205 154 262 3.38e-22 95.1
MsaG040886 AT5G45260.1 47.423 97 41 4 99 195 1188 1274 8.65e-19 88.2
MsaG040886 AT1G66550.1 29.282 181 90 6 16 184 13 167 5.63e-16 77.0
MsaG040886 AT1G66600.1 40.909 88 45 1 104 184 75 162 2.64e-15 74.7
MsaG040886 AT5G52830.1 54.839 62 26 1 126 187 166 225 3.16e-15 75.9
MsaG040886 AT1G29280.1 53.226 62 25 2 126 187 75 132 6.00e-15 73.9
MsaG040886 AT1G66550.2 38.636 88 47 1 104 184 75 162 1.35e-14 72.8
MsaG040886 AT4G31550.2 44.048 84 43 2 99 182 221 300 1.88e-14 73.6
MsaG040886 AT5G49520.1 50.000 64 29 1 124 187 220 280 2.21e-14 73.9
MsaG040886 AT4G31550.1 54.386 57 24 1 126 182 247 301 4.87e-14 72.4
MsaG040886 AT4G01250.1 51.613 62 26 2 126 187 129 186 8.80e-14 71.2
MsaG040886 AT1G30650.1 41.905 105 48 6 95 194 184 280 9.42e-14 72.0
MsaG040886 AT2G30590.1 50.794 63 27 2 126 188 314 372 9.82e-14 72.0
MsaG040886 AT2G34830.1 35.938 128 58 6 78 187 152 273 1.42e-13 71.6
MsaG040886 AT2G34830.2 35.938 128 58 6 78 187 161 282 1.50e-13 71.6
MsaG040886 AT1G66560.1 46.667 60 32 0 125 184 103 162 1.51e-13 70.1
MsaG040886 AT1G69310.4 40.476 84 47 1 99 182 121 201 3.97e-13 69.3
MsaG040886 AT1G69310.2 40.476 84 47 1 99 182 121 201 3.97e-13 69.3
MsaG040886 AT1G69310.1 40.476 84 47 1 99 182 121 201 3.97e-13 69.3
MsaG040886 AT2G24570.1 54.386 57 24 1 126 182 244 298 4.06e-13 69.7
MsaG040886 AT2G37260.3 35.345 116 60 3 72 182 218 323 4.90e-13 69.7
MsaG040886 AT2G37260.2 35.345 116 60 3 72 182 216 321 5.03e-13 69.3
MsaG040886 AT2G37260.1 35.345 116 60 3 72 182 298 403 5.88e-13 69.7
MsaG040886 AT5G41570.1 43.038 79 40 2 112 190 85 158 7.97e-13 66.6
MsaG040886 AT5G13080.1 51.562 64 26 2 124 187 66 124 8.48e-13 65.5
MsaG040886 AT3G58710.2 43.011 93 45 4 99 187 40 128 9.41e-13 67.8
MsaG040886 AT4G24240.1 41.667 84 45 2 99 182 257 336 1.12e-12 68.6
MsaG040886 AT1G29860.1 40.476 84 45 2 110 193 121 199 1.15e-12 67.8
MsaG040886 AT1G64000.1 40.659 91 43 3 112 196 101 186 1.23e-12 66.2
MsaG040886 AT2G23320.1 50.877 57 26 1 126 182 241 295 1.25e-12 68.2
MsaG040886 AT3G58710.1 50.000 62 27 2 126 187 72 129 1.40e-12 67.4
MsaG040886 AT4G18170.1 35.238 105 63 2 83 187 130 229 1.47e-12 67.8
MsaG040886 AT2G47260.1 37.778 90 53 1 98 187 147 233 1.50e-12 68.2
MsaG040886 AT2G46130.1 48.438 64 28 2 124 187 29 87 1.65e-12 63.5
MsaG040886 AT5G28650.1 52.632 57 25 1 126 182 263 317 1.78e-12 67.8
MsaG040886 AT4G23550.1 46.774 62 31 1 121 182 136 195 1.87e-12 67.4
MsaG040886 AT1G80590.1 39.241 79 45 1 112 187 69 147 2.80e-12 65.9
MsaG040886 AT2G44745.1 45.070 71 36 1 112 182 132 199 4.41e-12 65.1
MsaG040886 AT5G01900.1 43.750 64 36 0 126 189 111 174 5.44e-12 65.5
MsaG040886 AT3G01970.1 48.529 68 30 2 124 191 64 126 6.15e-12 63.2
MsaG040886 AT4G26640.7 45.714 70 32 3 125 194 171 234 6.19e-12 67.0
MsaG040886 AT4G26640.7 44.776 67 34 1 124 190 340 403 8.82e-12 66.2
MsaG040886 AT4G26640.1 45.070 71 33 3 124 194 138 202 6.90e-12 66.6
MsaG040886 AT4G26640.1 44.776 67 34 1 124 190 308 371 1.06e-11 66.2
MsaG040886 AT5G46350.1 46.875 64 29 2 124 187 182 240 6.94e-12 65.9
MsaG040886 AT4G26640.2 45.714 70 32 3 125 194 211 274 7.31e-12 66.6
MsaG040886 AT4G26640.2 44.776 67 34 1 124 190 380 443 1.10e-11 66.2
MsaG040886 AT4G26640.5 45.070 71 33 3 124 194 34 98 7.41e-12 66.2
MsaG040886 AT4G26640.5 44.776 67 34 1 124 190 204 267 1.22e-11 65.5
MsaG040886 AT4G26640.6 45.070 71 33 3 124 194 34 98 7.41e-12 66.2
MsaG040886 AT4G26640.6 44.776 67 34 1 124 190 204 267 1.22e-11 65.5
MsaG040886 AT4G26640.4 45.070 71 33 3 124 194 34 98 7.41e-12 66.2
MsaG040886 AT4G26640.4 44.776 67 34 1 124 190 204 267 1.22e-11 65.5
MsaG040886 AT4G26640.3 45.070 71 33 3 124 194 34 98 7.41e-12 66.2
MsaG040886 AT4G26640.3 44.776 67 34 1 124 190 204 267 1.22e-11 65.5
MsaG040886 AT3G01080.3 48.438 64 29 2 125 188 205 264 7.78e-12 65.9
MsaG040886 AT3G62340.1 48.438 64 30 1 124 187 117 177 7.99e-12 65.5
MsaG040886 AT3G04670.1 50.877 57 26 1 126 182 263 317 8.71e-12 65.9
MsaG040886 AT4G26440.1 50.794 63 27 2 125 187 178 236 9.61e-12 66.2
MsaG040886 AT4G26440.1 46.269 67 33 1 124 190 371 434 2.09e-11 65.5
MsaG040886 AT1G13960.1 46.875 64 30 2 125 188 229 288 1.01e-11 66.2
MsaG040886 AT1G13960.1 47.458 59 28 1 124 182 408 463 1.32e-11 65.9
MsaG040886 AT2G25000.3 44.776 67 35 1 123 189 123 187 1.15e-11 64.3
MsaG040886 AT3G01080.2 46.970 66 31 2 125 190 205 266 1.17e-11 65.9
MsaG040886 AT2G25000.2 44.776 67 35 1 123 189 101 165 1.22e-11 63.9
MsaG040886 AT2G25000.4 44.776 67 35 1 123 189 101 165 1.22e-11 63.9
MsaG040886 AT2G03340.1 45.455 66 32 2 125 190 250 311 1.29e-11 65.9
MsaG040886 AT2G03340.1 43.284 67 35 1 124 190 414 477 2.62e-11 65.1
MsaG040886 AT2G25000.1 44.776 67 35 1 123 189 144 208 1.45e-11 64.3
MsaG040886 AT3G01080.1 46.970 66 31 2 125 190 167 228 1.49e-11 65.5
MsaG040886 AT2G04880.2 44.304 79 36 4 119 197 105 175 1.79e-11 65.5
MsaG040886 AT2G04880.2 40.299 67 37 1 124 190 282 345 6.41e-11 63.5
MsaG040886 AT1G13960.2 47.458 59 28 1 124 182 255 310 1.83e-11 65.1
MsaG040886 AT1G13960.2 46.875 64 30 2 125 188 76 135 1.86e-11 64.7
MsaG040886 AT2G04880.1 44.304 79 36 4 119 197 105 175 1.84e-11 65.5
MsaG040886 AT2G04880.1 40.299 67 37 1 124 190 306 369 8.35e-11 63.2
MsaG040886 AT4G39410.1 41.176 85 46 2 98 182 197 277 2.94e-11 63.9
MsaG040886 AT4G01720.1 37.963 108 60 4 81 187 196 297 3.33e-11 64.7
MsaG040886 AT5G45050.4 42.500 80 40 3 108 187 1026 1099 4.69e-11 64.7
MsaG040886 AT5G45050.2 42.500 80 40 3 108 187 1137 1210 4.81e-11 64.7
MsaG040886 AT5G45050.1 42.500 80 40 3 108 187 1165 1238 4.83e-11 64.7
MsaG040886 AT5G07100.1 47.458 59 28 1 124 182 233 288 5.41e-11 63.2
MsaG040886 AT5G07100.1 42.857 70 35 2 125 194 117 181 8.91e-11 62.4
MsaG040886 AT5G07100.2 47.458 59 28 1 124 182 140 195 6.71e-11 61.6
MsaG040886 AT5G07100.2 42.857 70 35 2 125 194 24 88 9.03e-11 61.2
MsaG040886 AT5G07100.4 47.458 59 28 1 124 182 140 195 6.71e-11 61.6
MsaG040886 AT5G07100.4 42.857 70 35 2 125 194 24 88 9.03e-11 61.2
MsaG040886 AT5G07100.5 47.458 59 28 1 124 182 140 195 6.71e-11 61.6
MsaG040886 AT5G07100.5 42.857 70 35 2 125 194 24 88 9.03e-11 61.2
MsaG040886 AT5G07100.3 47.458 59 28 1 124 182 140 195 6.71e-11 61.6
MsaG040886 AT5G07100.3 42.857 70 35 2 125 194 24 88 9.03e-11 61.2
MsaG040886 AT2G21900.1 40.299 67 37 1 123 189 107 170 7.46e-11 61.2
MsaG040886 AT2G40740.2 45.902 61 33 0 145 205 149 209 7.69e-11 62.0
MsaG040886 AT5G43290.1 40.541 111 50 5 120 224 109 209 9.52e-11 62.0

Find 69 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTATGATGACCTCACTTT+TGG 0.188414 7:+104845916 None:intergenic
TCAACATAGTAAGTGCTTTC+TGG 0.207919 7:+104846014 None:intergenic
TCCCTCAAAGTTAAAGATAA+AGG 0.227041 7:-104844734 MsaT040886.1:CDS
CTCAAGCTAAGCCTTTAAAA+AGG 0.231958 7:-104844762 MsaT040886.1:CDS
ATTCTGAAGTTGGTCCATTT+TGG 0.232813 7:+104844476 None:intergenic
CAAGAATTCATTGGTTTCAT+TGG 0.239108 7:+104846060 None:intergenic
TTCACTTGTTCCTTCCATTT+AGG 0.269402 7:+104845562 None:intergenic
CAATTCACCAATCCTTGATT+TGG 0.271018 7:-104844413 MsaT040886.1:CDS
CATTGGATGATATAATTATA+TGG 0.275590 7:+104846077 None:intergenic
AGGATTATCTGAAGGGTCTT+TGG 0.285031 7:-104845524 MsaT040886.1:CDS
CCGAGGGAAAATACCCTCTT+TGG 0.291656 7:+104844600 None:intergenic
CTTTCCTTGGATTAATTCAT+TGG 0.302740 7:+104846123 None:intergenic
CAAATCAAGGATTGGTGAAT+TGG 0.310097 7:+104844414 None:intergenic
CCCTCAAAGTTAAAGATAAA+GGG 0.333691 7:-104844733 MsaT040886.1:CDS
CCCTTTATCTTTAACTTTGA+GGG 0.337711 7:+104844733 None:intergenic
GCAGAAAGAGCATGCCTAAA+TGG 0.338464 7:-104845576 MsaT040886.1:intron
TATGGTCAAAAGGATATTCT+TGG 0.360560 7:-104845478 MsaT040886.1:CDS
GAATATAGCTGGAGAAAATA+TGG 0.367378 7:-104845496 MsaT040886.1:CDS
GCCCTTTATCTTTAACTTTG+AGG 0.367772 7:+104844732 None:intergenic
TACAATGGATTATGCCAAAA+TGG 0.369767 7:-104844490 MsaT040886.1:CDS
TTTGAACCTGCTTTGTTGCT+AGG 0.370195 7:+104844848 None:intergenic
TAAGTTACCTCAATTATTGT+TGG 0.376442 7:+104844811 None:intergenic
TTTGTCAATCAAGAATTCAT+TGG 0.407531 7:+104846051 None:intergenic
GCTTTGCTAACTCCTTTCCT+TGG 0.408135 7:+104846110 None:intergenic
GGACTCTCGTTTGTAAAAGA+AGG 0.431784 7:+104845938 None:intergenic
TGGTTTGTCAGAATATCAAT+TGG 0.434536 7:-104844515 MsaT040886.1:CDS
GTGATATCAGATTCTGAAGT+TGG 0.434696 7:+104844466 None:intergenic
TACAGATGCACACATAGAAA+TGG 0.436912 7:-104844880 MsaT040886.1:CDS
TTTAACATCAAGTTCTGCCT+TGG 0.466903 7:+104844642 None:intergenic
GTAGCAAGATATCCAAATCA+AGG 0.467252 7:+104844401 None:intergenic
GGTGAATTGGTGACTGAAGT+TGG 0.470985 7:+104844427 None:intergenic
CTGGAGAAAATATGGTCAAA+AGG 0.471109 7:-104845488 MsaT040886.1:CDS
GGAGAGACTAAGACCATTGA+TGG 0.480847 7:-104845980 MsaT040886.1:CDS
GACCATTGATGGTAAAATGA+AGG 0.490394 7:-104845969 MsaT040886.1:CDS
GGTTTGTCAGAATATCAATT+GGG 0.490887 7:-104844514 MsaT040886.1:CDS
TCTGATTCAATTGATGGAGA+AGG 0.500124 7:+104844568 None:intergenic
ACAATGCTCTCTTCAAGAAA+AGG 0.501466 7:-104845880 MsaT040886.1:intron
CTTTGGATGATGAATATAGC+TGG 0.515960 7:-104845507 MsaT040886.1:CDS
ATCAGAAAATGAGATAATGA+TGG 0.516212 7:-104844551 MsaT040886.1:CDS
AAGATATCCAAATCAAGGAT+TGG 0.527680 7:+104844406 None:intergenic
GAGCATTGTGGTTAAACTCC+TGG 0.533299 7:+104845894 None:intergenic
GAATATCAATTGGGCTACAA+TGG 0.533841 7:-104844505 MsaT040886.1:CDS
TGAAGATCCAACAATAATTG+AGG 0.540253 7:-104844818 MsaT040886.1:CDS
TGGATATCTTGCTACAAAGA+GGG 0.541575 7:-104844393 MsaT040886.1:CDS
AGAGACTAGTTTCACTTCCA+AGG 0.541625 7:-104844659 MsaT040886.1:CDS
GGATATCTTGCTACAAAGAG+GGG 0.557765 7:-104844392 MsaT040886.1:CDS
TTGGATATCTTGCTACAAAG+AGG 0.559016 7:-104844394 MsaT040886.1:CDS
TAGCAACAAAGCAGGTTCAA+AGG 0.565187 7:-104844846 MsaT040886.1:CDS
AAATATTCAGGAGTATTGAG+AGG 0.566440 7:+104844352 None:intergenic
CATAGTAAGTGCTTTCTGGT+AGG 0.575281 7:+104846018 None:intergenic
CAATGAATTAATCCAAGGAA+AGG 0.585373 7:-104846122 MsaT040886.1:CDS
CTTACTATGTTGAATGTGAG+AGG 0.591736 7:-104846004 MsaT040886.1:CDS
AATATTCAGGAGTATTGAGA+GGG 0.594267 7:+104844353 None:intergenic
AAAGAGCATGCCTAAATGGA+AGG 0.601574 7:-104845572 MsaT040886.1:CDS
GTGAAGATTTGCACAAACAC+AGG 0.602509 7:-104845544 MsaT040886.1:CDS
ATAACCAATGAATTAATCCA+AGG 0.604601 7:-104846127 MsaT040886.1:CDS
GAGAAGGAAAACAAAACCGA+GGG 0.610739 7:+104844584 None:intergenic
AGGGTGCCTAGCAACAAAGC+AGG 0.623925 7:-104844854 MsaT040886.1:CDS
ACAAACACAGGATTATCTGA+AGG 0.625241 7:-104845532 MsaT040886.1:CDS
AAGTGAGGTCATCATAGACC+AGG 0.627405 7:-104845912 MsaT040886.1:CDS
CAAACACAGGATTATCTGAA+GGG 0.645284 7:-104845531 MsaT040886.1:CDS
AAACGAGAGTCCCAAAAGTG+AGG 0.654222 7:-104845927 MsaT040886.1:CDS
ATAATTGAGGTAACTTACAG+AGG 0.655185 7:-104844805 MsaT040886.1:CDS
GACTTAGAAGAAACCAAAGA+GGG 0.656480 7:-104844613 MsaT040886.1:CDS
GGAGAAGGAAAACAAAACCG+AGG 0.666774 7:+104844583 None:intergenic
ACTATGTTGAATGTGAGAGG+AGG 0.670945 7:-104846001 MsaT040886.1:CDS
TGACTTAGAAGAAACCAAAG+AGG 0.672282 7:-104844614 MsaT040886.1:CDS
TGCACACATAGAAATGGACA+AGG 0.697085 7:-104844874 MsaT040886.1:CDS
GCACACATAGAAATGGACAA+GGG 0.758330 7:-104844873 MsaT040886.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 104844342 104846182 104844342 ID=MsaG040886
Chr7 mRNA 104844342 104846182 104844342 ID=MsaT040886.1;Parent=MsaG040886
Chr7 exon 104844342 104844909 104844342 ID=MsaT040886.1.exon3;Parent=MsaT040886.1
Chr7 CDS 104844342 104844909 104844342 ID=cds.MsaT040886.1;Parent=MsaT040886.1
Chr7 exon 104845463 104845594 104845463 ID=MsaT040886.1.exon2;Parent=MsaT040886.1
Chr7 CDS 104845463 104845594 104845463 ID=cds.MsaT040886.1;Parent=MsaT040886.1
Chr7 exon 104845881 104846182 104845881 ID=MsaT040886.1.exon1;Parent=MsaT040886.1
Chr7 CDS 104845881 104846182 104845881 ID=cds.MsaT040886.1;Parent=MsaT040886.1
Gene Sequence

>MsaT040886.1

ATGGAAGAGACTACAAAGAGAAAACGTGTTAGTATAACCAATGAATTAATCCAAGGAAAGGAGTTAGCAAAGCAGCTAAGTAACCATATAATTATATCATCCAATGAAACCAATGAATTCTTGATTGACAAAATAATTTCAACCTACCAGAAAGCACTTACTATGTTGAATGTGAGAGGAGGAGAGACTAAGACCATTGATGGTAAAATGAAGGATTCTCATCCTTCTTTTACAAACGAGAGTCCCAAAAGTGAGGTCATCATAGACCAGGAGTTTAACCACAATGCTCTCTTCAAGAAAAGAAAGAGCATGCCTAAATGGAAGGAACAAGTGAAGATTTGCACAAACACAGGATTATCTGAAGGGTCTTTGGATGATGAATATAGCTGGAGAAAATATGGTCAAAAGGATATTCTTGGAGCTAAGTTTTCAAGAGGATATTACAGATGCACACATAGAAATGGACAAGGGTGCCTAGCAACAAAGCAGGTTCAAAGGTCAGATGAAGATCCAACAATAATTGAGGTAACTTACAGAGGAAGACACACATGCACTCATCCTCAAGCTAAGCCTTTAAAAAGGGAATTTCCCTCAAAGTTAAAGATAAAGGGCTTAGAGAAAAATAAACTTCACAATGATAAAAAGAATCAACTACAAACTCAAGAGACTAGTTTCACTTCCAAGGCAGAACTTGATGTTAAAAGAAATGACTTAGAAGAAACCAAAGAGGGTATTTTCCCTCGGTTTTGTTTTCCTTCTCCATCAATTGAATCAGAAAATGAGATAATGATGGAATCAAACTTTTTTGGTTTGTCAGAATATCAATTGGGCTACAATGGATTATGCCAAAATGGACCAACTTCAGAATCTGATATCACTGATACAGTTTCAGCTCCAACTTCAGTCACCAATTCACCAATCCTTGATTTGGATATCTTGCTACAAAGAGGGGATTTTGACACTGATTTCCCTCTCAATACTCCTGAATATTTCTCTTCATAA
Protein sequence

>MsaT040886.1

MEETTKRKRVSITNELIQGKELAKQLSNHIIISSNETNEFLIDKIISTYQKALTMLNVRGGETKTIDGKMKDSHPSFTNESPKSEVIIDQEFNHNALFKKRKSMPKWKEQVKICTNTGLSEGSLDDEYSWRKYGQKDILGAKFSRGYYRCTHRNGQGCLATKQVQRSDEDPTIIEVTYRGRHTCTHPQAKPLKREFPSKLKIKGLEKNKLHNDKKNQLQTQETSFTSKAELDVKRNDLEETKEGIFPRFCFPSPSIESENEIMMESNFFGLSEYQLGYNGLCQNGPTSESDITDTVSAPTSVTNSPILDLDILLQRGDFDTDFPLNTPEYFSS*