AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG040894

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040894 MtrunA17_Chr7g0275611 95.859 966 38 1 1 966 1 964 0.0 1927
MsaG040894 MtrunA17_Chr8g0347071 71.223 834 210 5 162 966 117 949 0.0 1249
MsaG040894 MtrunA17_Chr3g0126701 54.073 982 393 14 39 966 86 1063 0.0 1033
MsaG040894 MtrunA17_Chr1g0147121 54.211 950 387 10 57 966 93 1034 0.0 1007
MsaG040894 MtrunA17_Chr7g0221611 31.884 483 258 15 526 966 592 1045 2.84e-53 202
MsaG040894 MtrunA17_Chr1g0149611 29.457 387 218 15 597 966 160 508 7.78e-36 143
MsaG040894 MtrunA17_Chr1g0149581 47.183 142 68 4 830 966 1527 1666 3.45e-30 129
MsaG040894 MtrunA17_Chr2g0297191 41.958 143 78 3 825 966 1033 1171 4.06e-29 125
MsaG040894 MtrunA17_Chr1g0201621 38.816 152 87 4 816 965 76 223 2.82e-20 95.5
MsaG040894 MtrunA17_Chr1g0194631 38.028 142 72 4 825 953 611 749 9.57e-20 95.1
MsaG040894 MtrunA17_Chr1g0201311 38.158 152 88 4 816 965 76 223 1.29e-19 93.2
MsaG040894 MtrunA17_Chr5g0401921 34.559 136 82 3 825 960 715 843 1.82e-18 90.9
MsaG040894 MtrunA17_Chr6g0477001 38.776 147 75 4 818 963 116 248 2.50e-18 87.8
MsaG040894 MtrunA17_Chr2g0305371 35.766 137 83 3 830 965 339 471 9.33e-18 87.8
MsaG040894 MtrunA17_Chr3g0115211 32.222 180 105 6 538 703 293 469 1.74e-17 88.2
MsaG040894 MtrunA17_Chr3g0125251 34.810 158 86 5 816 965 1316 1464 1.47e-15 82.0
MsaG040894 MtrunA17_Chr8g0359281 28.755 233 138 9 485 703 82 300 3.20e-15 78.2
MsaG040894 MtrunA17_Chr7g0235081 32.117 137 89 3 813 947 183 317 5.35e-15 77.8
MsaG040894 MtrunA17_Chr7g0270001 33.065 124 79 3 825 947 149 269 6.05e-15 76.6
MsaG040894 MtrunA17_Chr5g0399801 31.667 180 80 7 823 965 915 1088 2.81e-12 71.2
MsaG040894 MtrunA17_Chr4g0003501 30.065 153 71 3 823 945 783 929 2.58e-11 67.8
MsaG040894 MtrunA17_Chr4g0019381 38.571 70 40 1 341 407 268 337 3.32e-11 67.4
MsaG040894 MtrunA17_Chr7g0232981 31.902 163 80 6 830 961 349 511 3.62e-11 67.0
MsaG040894 MtrunA17_Chr4g0003511 30.323 155 70 4 823 945 878 1026 4.95e-11 67.0
MsaG040894 MtrunA17_Chr7g0232811 30.508 177 78 7 830 961 358 534 9.80e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG040894 AT3G61740.2 56.757 925 364 12 60 966 76 982 0.0 1068
MsaG040894 AT3G61740.1 54.479 960 367 11 60 966 76 1018 0.0 1054
MsaG040894 AT5G53430.1 51.499 967 393 10 57 966 96 1043 0.0 984
MsaG040894 AT4G27910.1 52.266 949 394 12 57 966 99 1027 0.0 964
MsaG040894 AT1G05830.2 26.643 837 476 32 179 966 312 1059 2.86e-64 236
MsaG040894 AT1G05830.4 26.643 837 476 32 179 966 312 1059 2.86e-64 236
MsaG040894 AT1G05830.1 26.643 837 476 32 179 966 312 1059 2.86e-64 236
MsaG040894 AT1G05830.3 26.643 837 476 32 179 966 312 1059 2.86e-64 236
MsaG040894 AT2G31650.1 31.325 498 287 15 496 966 569 1038 5.64e-64 235
MsaG040894 AT5G42400.7 38.323 167 90 4 801 966 1234 1388 2.54e-28 124
MsaG040894 AT5G42400.2 38.323 167 90 4 801 966 1234 1388 2.54e-28 124
MsaG040894 AT5G42400.8 38.323 167 90 4 801 966 1234 1388 2.54e-28 124
MsaG040894 AT5G42400.5 38.323 167 90 4 801 966 1234 1388 2.54e-28 124
MsaG040894 AT5G42400.4 38.323 167 90 4 801 966 1234 1388 2.54e-28 124
MsaG040894 AT5G42400.1 38.323 167 90 4 801 966 1234 1388 2.54e-28 124
MsaG040894 AT5G42400.3 38.323 167 90 4 801 966 1234 1388 2.54e-28 124
MsaG040894 AT5G42400.6 38.323 167 90 4 801 966 1234 1388 2.54e-28 124
MsaG040894 AT1G76710.3 40.000 150 84 4 818 965 83 228 2.93e-19 92.8
MsaG040894 AT1G76710.2 40.000 150 84 4 818 965 83 228 3.01e-19 92.8
MsaG040894 AT1G76710.1 40.000 150 84 4 818 965 83 228 3.01e-19 92.8
MsaG040894 AT1G76710.4 40.000 150 84 4 818 965 83 228 3.35e-19 92.8
MsaG040894 AT3G14740.2 34.568 162 93 7 538 690 153 310 4.93e-18 87.4
MsaG040894 AT3G14740.1 34.568 162 93 7 538 690 153 310 4.99e-18 87.0
MsaG040894 AT2G44150.1 39.231 130 74 3 835 963 127 252 6.70e-18 87.0
MsaG040894 AT2G23380.2 38.686 137 73 5 825 959 526 653 1.02e-17 89.0
MsaG040894 AT4G30860.1 35.484 155 85 4 812 965 324 464 1.06e-17 87.8
MsaG040894 AT2G23380.1 38.686 137 73 5 825 959 753 880 1.33e-17 89.0
MsaG040894 AT3G59960.3 38.931 131 73 4 835 963 122 247 2.26e-17 84.3
MsaG040894 AT3G59960.2 38.931 131 73 4 835 963 122 247 2.26e-17 84.3
MsaG040894 AT3G59960.4 38.931 131 73 4 835 963 122 247 2.26e-17 84.3
MsaG040894 AT1G02580.1 37.984 129 72 4 823 948 543 666 4.17e-17 86.7
MsaG040894 AT3G59960.1 39.695 131 72 4 835 963 122 247 4.28e-17 84.3
MsaG040894 AT5G28340.1 59.677 62 24 1 317 378 16 76 4.32e-17 85.5
MsaG040894 AT4G02020.1 36.585 123 74 3 825 946 708 827 5.00e-17 87.0
MsaG040894 AT1G77800.7 32.335 167 96 6 550 703 293 455 7.55e-17 86.7
MsaG040894 AT1G77800.4 32.335 167 96 6 550 703 293 455 9.11e-17 86.3
MsaG040894 AT1G77800.5 32.335 167 96 6 550 703 293 455 9.44e-17 86.3
MsaG040894 AT1G77800.3 32.335 167 96 6 550 703 293 455 9.59e-17 86.3
MsaG040894 AT1G77800.6 32.335 167 96 6 550 703 293 455 1.02e-16 86.3
MsaG040894 AT2G23740.3 34.940 166 90 4 818 966 1218 1382 3.34e-16 84.3
MsaG040894 AT2G23740.6 34.940 166 90 4 818 966 1218 1382 3.34e-16 84.3
MsaG040894 AT2G23740.2 34.940 166 90 4 818 966 1218 1382 3.34e-16 84.3
MsaG040894 AT2G23740.1 35.329 167 88 5 818 966 1211 1375 3.45e-16 84.3
MsaG040894 AT2G35160.5 37.398 123 65 3 830 946 617 733 1.48e-14 78.6
MsaG040894 AT2G35160.2 37.398 123 65 3 830 946 653 769 1.58e-14 78.6
MsaG040894 AT2G35160.3 37.398 123 65 3 830 946 653 769 1.58e-14 78.6
MsaG040894 AT2G35160.1 37.398 123 65 3 830 946 653 769 1.58e-14 78.6
MsaG040894 AT2G35160.4 37.398 123 65 3 830 946 653 769 1.58e-14 78.6
MsaG040894 AT1G77300.2 32.639 144 80 5 830 965 1032 1166 8.32e-14 76.6
MsaG040894 AT1G77300.1 31.013 158 92 5 816 965 1018 1166 9.07e-14 76.6
MsaG040894 AT1G77800.1 34.400 125 69 4 550 663 293 415 8.16e-13 73.6
MsaG040894 AT1G77800.2 34.400 125 69 4 550 663 293 415 8.23e-13 73.6
MsaG040894 AT3G61723.1 70.270 37 11 0 369 405 3 39 6.44e-12 62.4
MsaG040894 AT5G13960.2 42.697 89 43 2 883 963 399 487 2.41e-11 67.8
MsaG040894 AT4G02020.4 34.831 89 57 1 825 913 708 795 2.74e-11 68.2
MsaG040894 AT5G13960.1 42.697 89 43 2 885 965 535 623 3.29e-11 67.8

Find 225 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGCAACCACTAATAAAA+TGG 0.142705 7:-104956184 MsaT040894.1:CDS
TGGAATTAGATTTGTAAATA+AGG 0.165304 7:-104958851 MsaT040894.1:CDS
AATGAAAATAAGTACCTTAT+TGG 0.189840 7:+104953695 None:intergenic
TTCATTTGGGTGATATAGTT+TGG 0.229686 7:-104958630 MsaT040894.1:CDS
ACCATTACTGAATCTGGATT+TGG 0.254575 7:+104953897 None:intergenic
CTTATGAAAGTCACTTTCTT+TGG 0.255575 7:+104958183 None:intergenic
GTTCATAAATTTCCTACAAT+TGG 0.271969 7:+104950618 None:intergenic
CCTATGGTTTACCTTCAAAA+AGG 0.279530 7:+104954708 None:intergenic
ATGAACCAGTTTATGCAAAA+TGG 0.284906 7:-104955708 MsaT040894.1:CDS
GTTAAAGCGGAGAGTGAAAA+TGG 0.287170 7:-104958703 MsaT040894.1:CDS
TCCGACTTTCCCTTGCAATC+TGG 0.289613 7:+104956945 None:intergenic
ATTCCCCTTCTTGGAATTCA+TGG 0.292080 7:-104958329 MsaT040894.1:CDS
GACTCGGGAGCTTGCAAAAT+AGG 0.294700 7:+104958559 None:intergenic
GTAAAACGCGTTATTGAGTT+TGG 0.296638 7:+104959092 None:intergenic
TTCATAAATTTCCTACAATT+GGG 0.299969 7:+104950619 None:intergenic
GGGGATGGTATTCCCCTTCT+TGG 0.300688 7:-104958338 MsaT040894.1:CDS
AATCTCAAGGGACGCTTTAG+AGG 0.311492 7:-104953828 MsaT040894.1:CDS
CTGTTTCTGACTTAACTTCA+TGG 0.319053 7:+104955909 None:intergenic
GGAATTAGATTTGTAAATAA+GGG 0.323627 7:-104958850 MsaT040894.1:CDS
CAAAACCATGAAGAAGATTA+AGG 0.323746 7:-104957489 MsaT040894.1:CDS
TGAATCCTTAATCTTCTTCA+TGG 0.325520 7:+104957484 None:intergenic
TGCACAACCATTACTGAATC+TGG 0.325878 7:+104953891 None:intergenic
CACCGATAATTCACTTGCTT+GGG 0.328806 7:-104953500 MsaT040894.1:CDS
AATATTCTTGTTCAGATTCC+AGG 0.333356 7:-104958227 MsaT040894.1:intron
GGACTATGCGTGGGCGAAGC+AGG 0.334035 7:-104958359 MsaT040894.1:CDS
TTTCAGGCAACACAGGTCTT+TGG 0.334623 7:+104956760 None:intergenic
TAAAGGGAGTCGTATGTTAT+TGG 0.335990 7:-104958725 MsaT040894.1:CDS
TGTTGCCTCTGTCCAGTAAA+AGG 0.341057 7:-104955363 MsaT040894.1:intron
GGAACGCCTTCACCATTTAC+AGG 0.344018 7:-104953301 MsaT040894.1:intron
GTTCCATTGCCTCATGGTTT+CGG 0.344780 7:+104954764 None:intergenic
TTCTTCACCAGCAGAAATAT+TGG 0.348515 7:+104950843 None:intergenic
CAATACCTTGAAACGTTTGC+TGG 0.351475 7:+104957102 None:intergenic
CCGACTTTCCCTTGCAATCT+GGG 0.351562 7:+104956946 None:intergenic
ACCATTCTGATGCTGGTGAT+TGG 0.353884 7:-104957278 MsaT040894.1:CDS
TACATGCTTACCTTGCAAAA+AGG 0.353988 7:+104955879 None:intergenic
CTTCATGGAAATTCCAGCTT+GGG 0.354837 7:+104955924 None:intergenic
AATGGTTGTGCATACCCCTC+TGG 0.357601 7:-104953880 MsaT040894.1:CDS
CAAATCGGTGGAAGCTGAAT+TGG 0.357759 7:+104959044 None:intergenic
TGCAACTTCCTGTGCTGAAC+AGG 0.359608 7:-104958086 MsaT040894.1:CDS
AAGACTTACAAATGAAGCTT+TGG 0.359930 7:+104956696 None:intergenic
TTTCTTTGGTCTTGTACAAT+CGG 0.367622 7:+104958197 None:intergenic
CATGAAGTTAAGTCAGAAAC+AGG 0.370698 7:-104955908 MsaT040894.1:CDS
GAAATTAACTGACCTGTAAA+TGG 0.372528 7:+104953289 None:intergenic
CTGTGATGGTTGTAATGTTT+GGG 0.373170 7:-104957149 MsaT040894.1:CDS
GCTGTGATGGTTGTAATGTT+TGG 0.373984 7:-104957150 MsaT040894.1:CDS
GCACTTAAAGAATGAAGGTT+TGG 0.378085 7:+104953474 None:intergenic
TCATTTGGGTGATATAGTTT+GGG 0.379404 7:-104958629 MsaT040894.1:CDS
GTTGGCAGATAATGGCATTC+TGG 0.384003 7:-104958143 MsaT040894.1:CDS
ATGAAAATAAGTACCTTATT+GGG 0.385620 7:+104953696 None:intergenic
CTTTGCAAGGATTATGTGCT+TGG 0.395078 7:-104950902 MsaT040894.1:CDS
AATTTCCAGCAAACGTTTCA+AGG 0.395311 7:-104957107 MsaT040894.1:intron
ACAAGATTATTATATGTAAC+AGG 0.395489 7:-104955636 MsaT040894.1:intron
TTTGCAGATGGGTAGAAGAT+TGG 0.403813 7:-104955666 MsaT040894.1:CDS
CCATTACTGAATCTGGATTT+GGG 0.409163 7:+104953898 None:intergenic
CCATTCTGATGCTGGTGATT+GGG 0.411055 7:-104957277 MsaT040894.1:intron
ATGTAACATGTGCTTGGTTC+CGG 0.413099 7:-104954801 MsaT040894.1:CDS
TGGTTTCGGAAAACAACTTC+AGG 0.413514 7:+104954778 None:intergenic
AATGCTTCCATCCAAGTTCA+AGG 0.415327 7:-104958965 MsaT040894.1:CDS
GAGGCAATGGAACCTGCCTC+TGG 0.415893 7:-104954754 MsaT040894.1:CDS
TAAAACGCGTTATTGAGTTT+GGG 0.416320 7:+104959093 None:intergenic
TTCAGCTACAATATTCAAAT+CGG 0.416513 7:+104959029 None:intergenic
AACAATCTTTGCCTTCTAAC+CGG 0.416708 7:+104952831 None:intergenic
TTCAACCTCTGAACATTATG+AGG 0.416920 7:-104953763 MsaT040894.1:CDS
GAGGCAATGTTGGCAGATAA+TGG 0.417160 7:-104958151 MsaT040894.1:CDS
TTGTAGGAAATTTATGAACT+AGG 0.418090 7:-104950614 None:intergenic
ACCAGCAGAAATATTGGTCT+TGG 0.419654 7:+104950849 None:intergenic
TTACAAAAGGCCTCCCAATA+AGG 0.422726 7:-104953709 MsaT040894.1:intron
AAAGCAGGCATTGGCTGAAT+GGG 0.423928 7:-104956264 MsaT040894.1:CDS
ATAATCCTTGCAAAGCAGTT+TGG 0.424513 7:+104950910 None:intergenic
AGGAACTAGGAGCAATCTTA+AGG 0.428043 7:+104954728 None:intergenic
GCACCGATAATTCACTTGCT+TGG 0.430380 7:-104953501 MsaT040894.1:CDS
GGTAATTGATGCAACTGATA+AGG 0.433248 7:-104951123 MsaT040894.1:CDS
TTGCAGATGGGTAGAAGATT+GGG 0.436806 7:-104955665 MsaT040894.1:CDS
ATGGTTGTGCATACCCCTCT+GGG 0.436853 7:-104953879 MsaT040894.1:CDS
GGCTTCTTCTGAATCTGTGA+TGG 0.439726 7:+104959114 None:intergenic
AAAAGCAGGCATTGGCTGAA+TGG 0.444691 7:-104956265 MsaT040894.1:CDS
TCTTTGCACCATAGCACTCT+TGG 0.448316 7:+104955448 None:intergenic
CTTGGAAAATCAGCTATTCA+AGG 0.449530 7:-104953064 MsaT040894.1:CDS
AAACCCAGGAAGTAAAATCT+TGG 0.450551 7:+104955421 None:intergenic
GATTATGTGCTTGGGAGATC+AGG 0.453525 7:-104950893 MsaT040894.1:CDS
AATGCAACCACTAATAAAAT+GGG 0.456087 7:-104956183 MsaT040894.1:intron
GAAGCAAAGTACCGGTTAGA+AGG 0.458443 7:-104952842 MsaT040894.1:CDS
GTTCAGAGGTTGAAGGTTCA+AGG 0.458605 7:+104953772 None:intergenic
GTAATTGATGCAACTGATAA+GGG 0.460608 7:-104951122 MsaT040894.1:CDS
CAGGATACAATATCTTGTTA+TGG 0.461186 7:-104957533 MsaT040894.1:CDS
TTCTTTGGTCTTGTACAATC+GGG 0.464370 7:+104958198 None:intergenic
TTCAAGGACTCTGTACTTCT+CGG 0.464616 7:-104958949 MsaT040894.1:CDS
CGACACTTGGCGGCAGATAG+TGG 0.465133 7:+104953735 None:intergenic
CAACACTCTCTTTCTACATC+AGG 0.465853 7:+104955381 None:intergenic
CCTTCTTGGAATTCATGGAT+AGG 0.467367 7:-104958324 MsaT040894.1:intron
AGTATCGTGGTGAACAGTTA+AGG 0.469535 7:-104952886 MsaT040894.1:CDS
AAACATAGTGTGAAAGTAGA+AGG 0.474293 7:-104956209 MsaT040894.1:CDS
AAGCTCCCGAGTCTGTTCTT+AGG 0.475308 7:-104958549 MsaT040894.1:CDS
TTGCTTTGTGATAGGATGCC+GGG 0.477967 7:-104953341 MsaT040894.1:intron
GCCAAGACCAATATTTCTGC+TGG 0.481706 7:-104950850 MsaT040894.1:CDS
AAATTAAAATCACCTGGCCT+TGG 0.481839 7:+104958053 None:intergenic
CAAAGAAAGTGACTTTCATA+AGG 0.485645 7:-104958182 MsaT040894.1:CDS
TAATATATTGTGCAGTTCAC+AGG 0.486204 7:-104954010 MsaT040894.1:intron
TTATTGCACTTAAAGAATGA+AGG 0.488618 7:+104953469 None:intergenic
AACATAACACAAATTGCATC+GGG 0.489497 7:+104958517 None:intergenic
AGAGATGTGCGGTTTGCAGA+TGG 0.489992 7:-104955678 MsaT040894.1:CDS
CCAAGTGTCGTGTTTACAAA+AGG 0.490232 7:-104953722 MsaT040894.1:CDS
CTCGTAGAGATTTGCAAGAA+GGG 0.490722 7:-104953027 MsaT040894.1:CDS
TTTGCAAGGATTATGTGCTT+GGG 0.492081 7:-104950901 MsaT040894.1:CDS
TGTACAATCGGGTATGCCCC+TGG 0.493229 7:+104958209 None:intergenic
TTTGCTTTGTGATAGGATGC+CGG 0.495752 7:-104953342 MsaT040894.1:intron
GACTATGCGTGGGCGAAGCA+GGG 0.495864 7:-104958358 MsaT040894.1:CDS
GGCAATGGAGGAGGCAATGT+TGG 0.497734 7:-104958161 MsaT040894.1:CDS
GCTCGTAGAGATTTGCAAGA+AGG 0.505407 7:-104953028 MsaT040894.1:CDS
AGGCAGGTTCCATTGCCTCA+TGG 0.506155 7:+104954758 None:intergenic
CCTATCCATGAATTCCAAGA+AGG 0.508824 7:+104958324 None:intergenic
ACTTCATGGAAATTCCAGCT+TGG 0.515022 7:+104955923 None:intergenic
AGCTACAATATTCAAATCGG+TGG 0.516229 7:+104959032 None:intergenic
GAAGGTGAGAATAGTGACAT+TGG 0.517945 7:-104958871 MsaT040894.1:CDS
GCTGAATGGGAACGGCATAC+AGG 0.518383 7:-104956251 MsaT040894.1:CDS
TCATAAATTTCCTACAATTG+GGG 0.519144 7:+104950620 None:intergenic
GTTCAAGATTAGTGAGGAAG+TGG 0.523406 7:-104951144 MsaT040894.1:CDS
GAAAATTGTAGAGTCTGCCT+TGG 0.524458 7:-104953082 MsaT040894.1:CDS
AAACATAACACAAATTGCAT+CGG 0.525202 7:+104958516 None:intergenic
AACTCAATTGAACAACAATA+CGG 0.527937 7:-104953448 MsaT040894.1:intron
TAGATTTGTAAATAAGGGAA+AGG 0.528339 7:-104958845 MsaT040894.1:CDS
ATCTTAAGGATTCCAGAGGC+AGG 0.529969 7:+104954742 None:intergenic
AAGTACAGAGTCCTTGAACT+TGG 0.530305 7:+104958954 None:intergenic
GGAATATCACTGCCAAGGCC+AGG 0.530950 7:-104958065 MsaT040894.1:intron
GTTGCGTTCTACAGTGAAGA+AGG 0.532356 7:-104954054 MsaT040894.1:CDS
ACAGAGTCCTTGAACTTGGA+TGG 0.532483 7:+104958958 None:intergenic
ATTTGGCACCATTCTGATGC+TGG 0.532842 7:-104957285 MsaT040894.1:CDS
AACTGATGTTGAGTTGCTGT+GGG 0.534511 7:-104954827 MsaT040894.1:CDS
ATATTCTTGTTCAGATTCCA+GGG 0.535307 7:-104958226 MsaT040894.1:intron
ACTCTTGGTGAACAGCAACT+TGG 0.536090 7:+104955463 None:intergenic
GTGATATTCCTGTTCAGCAC+AGG 0.536188 7:+104958078 None:intergenic
ACACTTGCAACAACTTGTAC+AGG 0.537991 7:+104954335 None:intergenic
ACAATATCTTGTTATGGCTG+CGG 0.538001 7:-104957527 MsaT040894.1:CDS
ACGGAACACAATCCCCAAGC+TGG 0.538151 7:-104955937 MsaT040894.1:CDS
TGATTTGTATGTTTGTGATG+TGG 0.538202 7:+104956911 None:intergenic
TTTGTAAACACGACACTTGG+CGG 0.538471 7:+104953725 None:intergenic
TTACTATTGCCCAGATTGCA+AGG 0.539807 7:-104956955 MsaT040894.1:CDS
CCCAATCACCAGCATCAGAA+TGG 0.540703 7:+104957277 None:intergenic
TTGTGCAAGTGTAGCACCTG+TGG 0.541587 7:-104956293 MsaT040894.1:CDS
CCTCTGGATGATCGCGACAA+TGG 0.541959 7:+104958997 None:intergenic
ATAGGATCAATGACGACAGC+AGG 0.545693 7:+104958577 None:intergenic
GGCGTAGTGTTGCTGATCTG+AGG 0.547095 7:-104952865 MsaT040894.1:CDS
CTTTCATAAGGCAATGGAGG+AGG 0.548985 7:-104958170 MsaT040894.1:CDS
AACTTCACTTCAAAATCTCA+AGG 0.550311 7:-104953841 MsaT040894.1:CDS
CTATCCATGAATTCCAAGAA+GGG 0.555475 7:+104958325 None:intergenic
AAATCAGCTATTCAAGGGTG+GGG 0.556616 7:-104953058 MsaT040894.1:CDS
CATAAATTTCCTACAATTGG+GGG 0.557442 7:+104950621 None:intergenic
GCTTGGGAGATCAGGAGAGC+AGG 0.561803 7:-104950885 MsaT040894.1:CDS
ATGACGACAGCAGGCCATGC+CGG 0.561841 7:+104958586 None:intergenic
CACAGCAACTCAACATCAGT+TGG 0.562296 7:+104954829 None:intergenic
GAGATGTGCGGTTTGCAGAT+GGG 0.562589 7:-104955677 MsaT040894.1:CDS
TTTCCGAAACCATGAGGCAA+TGG 0.563563 7:-104954767 MsaT040894.1:CDS
AAAATCAGCTATTCAAGGGT+GGG 0.563568 7:-104953059 MsaT040894.1:CDS
TTCATGGAAATTCCAGCTTG+GGG 0.563704 7:+104955925 None:intergenic
GGGTTCATGTAACATGTGCT+TGG 0.565060 7:-104954807 MsaT040894.1:CDS
GCGTAGTGTTGCTGATCTGA+GGG 0.565380 7:-104952864 MsaT040894.1:CDS
ACCGATAATTCACTTGCTTG+GGG 0.568704 7:-104953499 MsaT040894.1:CDS
ATCTGAGGGAAGCAAAGTAC+CGG 0.572325 7:-104952850 MsaT040894.1:CDS
GCATGCCAAACTGCTTTGCA+AGG 0.572847 7:-104950915 MsaT040894.1:CDS
CTGACTTAGGTATGCTGTGA+TGG 0.573283 7:-104957163 MsaT040894.1:intron
AACTGACCTGTAAATGGTGA+AGG 0.574216 7:+104953295 None:intergenic
AGGCATTGGCTGAATGGGAA+CGG 0.575274 7:-104956259 MsaT040894.1:CDS
ACTATGCGTGGGCGAAGCAG+GGG 0.575564 7:-104958357 MsaT040894.1:CDS
CCCAAATCCAGATTCAGTAA+TGG 0.577133 7:-104953898 MsaT040894.1:CDS
TGTGCAAGTGTAGCACCTGT+GGG 0.580364 7:-104956292 MsaT040894.1:CDS
AAGTGACTTTCATAAGGCAA+TGG 0.583677 7:-104958176 MsaT040894.1:CDS
CCCAGATTGCAAGGGAAAGT+CGG 0.583861 7:-104956946 MsaT040894.1:CDS
TGTGATGTGTGCATCAAGGA+TGG 0.584210 7:-104954298 MsaT040894.1:CDS
TTCGGAAAACAACTTCAGGC+CGG 0.585949 7:+104954782 None:intergenic
TCATAATGTTCAGAGGTTGA+AGG 0.587056 7:+104953765 None:intergenic
CAACTGATGTTGAGTTGCTG+TGG 0.587107 7:-104954828 MsaT040894.1:CDS
TTATAGAGCTGAGCAAATTG+TGG 0.590020 7:-104958113 MsaT040894.1:CDS
TTGCGTTCTACAGTGAAGAA+GGG 0.590318 7:-104954053 MsaT040894.1:CDS
TCGTATGTTATTGGTTAAAG+CGG 0.592806 7:-104958716 MsaT040894.1:CDS
GTGGTGTGCAATGGCTTGAA+AGG 0.593816 7:-104956729 MsaT040894.1:CDS
ATTTGCTCAGCTCTATAATG+TGG 0.594776 7:+104958118 None:intergenic
CGGTGGAAGCTGAATTGGAG+TGG 0.597678 7:+104959049 None:intergenic
TGGAGTGGATCTCGCCATCA+CGG 0.597706 7:+104959064 None:intergenic
TACTATTGCCCAGATTGCAA+GGG 0.599167 7:-104956954 MsaT040894.1:CDS
GATATAGTTTGGGCAAAATG+TGG 0.600657 7:-104958619 MsaT040894.1:CDS
TGCGTGGGCGAAGCAGGGGA+TGG 0.600857 7:-104958353 MsaT040894.1:CDS
TTCATGTGATGTGTGCATCA+AGG 0.601157 7:-104954302 MsaT040894.1:CDS
ATCAAGGATGGGATATAGCA+TGG 0.605078 7:-104954286 MsaT040894.1:CDS
CCGATAATTCACTTGCTTGG+GGG 0.606891 7:-104953498 MsaT040894.1:CDS
CAAATCAAAAGATCTAAGCT+CGG 0.607829 7:-104958898 MsaT040894.1:CDS
TTATTGTAAATCTTGTGCAA+AGG 0.607958 7:-104957444 MsaT040894.1:intron
TTCACACACTCTACAAACCC+AGG 0.608046 7:+104955407 None:intergenic
GAAAATCAGCTATTCAAGGG+TGG 0.611381 7:-104953060 MsaT040894.1:CDS
CTGGATGATCGCGACAATGG+AGG 0.613844 7:+104959000 None:intergenic
ACAGCGCCTAAGAACAGACT+CGG 0.613960 7:+104958543 None:intergenic
TATCCATGAATTCCAAGAAG+GGG 0.615664 7:+104958326 None:intergenic
AGATTTGCAAGAAGGGGACA+TGG 0.615755 7:-104953020 MsaT040894.1:intron
TTGGAAAATCAGCTATTCAA+GGG 0.617569 7:-104953063 MsaT040894.1:CDS
TGACTTTCATAAGGCAATGG+AGG 0.617576 7:-104958173 MsaT040894.1:CDS
ACTTCACTTCAAAATCTCAA+GGG 0.617691 7:-104953840 MsaT040894.1:CDS
TCGTAGAGATTTGCAAGAAG+GGG 0.617837 7:-104953026 MsaT040894.1:CDS
GAACAGGAATATCACTGCCA+AGG 0.624061 7:-104958070 MsaT040894.1:CDS
AAAATAAGTACCTTATTGGG+AGG 0.624661 7:+104953699 None:intergenic
TGCGTTCTACAGTGAAGAAG+GGG 0.630161 7:-104954052 MsaT040894.1:CDS
CTCAACCTCATAATGTTCAG+AGG 0.631848 7:+104953758 None:intergenic
TAGAGCTGAGCAAATTGTGG+AGG 0.632383 7:-104958110 MsaT040894.1:CDS
GGTTGTGCATACCCCTCTGG+GGG 0.635590 7:-104953877 MsaT040894.1:CDS
AACAACTTGTACAGGAACCA+TGG 0.635744 7:+104954343 None:intergenic
GCACCTGGCAGGACTATGCG+TGG 0.636994 7:-104958369 MsaT040894.1:intron
AATCTAGCGGTGGTGTGCAA+TGG 0.638103 7:-104956738 MsaT040894.1:CDS
TGGTTGTGCATACCCCTCTG+GGG 0.639129 7:-104953878 MsaT040894.1:CDS
GCTGTTCACCAAGAGTGCTA+TGG 0.641660 7:-104955456 MsaT040894.1:CDS
CAGCGCCTAAGAACAGACTC+GGG 0.643241 7:+104958544 None:intergenic
AGATGAGCGAGATGAACTCA+AGG 0.647248 7:-104950662 MsaT040894.1:CDS
TGCTTTGTGATAGGATGCCG+GGG 0.652047 7:-104953340 MsaT040894.1:intron
GCAGCTGTTCAAGATTAGTG+AGG 0.658448 7:-104951150 MsaT040894.1:intron
AGAGCTGAGCAAATTGTGGA+GGG 0.658542 7:-104958109 MsaT040894.1:CDS
CTGCAGGTTGTTGAGTATCG+TGG 0.658893 7:-104952899 MsaT040894.1:intron
TATTCTTGTTCAGATTCCAG+GGG 0.660445 7:-104958225 MsaT040894.1:intron
CACCTGGCAGGACTATGCGT+GGG 0.661987 7:-104958368 MsaT040894.1:intron
CAAGATTTACAATAATGCTG+AGG 0.678605 7:+104957452 None:intergenic
GTGATGTGTGCATCAAGGAT+GGG 0.680804 7:-104954297 MsaT040894.1:CDS
GTGATCTGTAAACAAAGCCA+TGG 0.684670 7:-104954360 MsaT040894.1:CDS
GTTGCCTGAAAATCTAGCGG+TGG 0.688256 7:-104956748 MsaT040894.1:CDS
AGCAATCTTAAGGATTCCAG+AGG 0.695160 7:+104954738 None:intergenic
CTTAAATCGAAACAATACTG+TGG 0.695578 7:-104957321 MsaT040894.1:CDS
CCATTGTCGCGATCATCCAG+AGG 0.697602 7:-104958997 MsaT040894.1:CDS
TGTGTTGCCTGAAAATCTAG+CGG 0.699729 7:-104956751 MsaT040894.1:CDS
CTTAAATGAAGAAAACACCC+CGG 0.721817 7:+104953323 None:intergenic
ATGGACAACAGAGAGATGTG+CGG 0.745685 7:-104955689 MsaT040894.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 104950615 104959190 104950615 ID=MsaG040894
Chr7 mRNA 104950615 104959190 104950615 ID=MsaT040894.1;Parent=MsaG040894
Chr7 exon 104950615 104950705 104950615 ID=MsaT040894.1.exon23;Parent=MsaT040894.1
Chr7 CDS 104950615 104950705 104950615 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104950838 104950938 104950838 ID=MsaT040894.1.exon22;Parent=MsaT040894.1
Chr7 CDS 104950838 104950938 104950838 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104951094 104951168 104951094 ID=MsaT040894.1.exon21;Parent=MsaT040894.1
Chr7 CDS 104951094 104951168 104951094 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104952829 104952915 104952829 ID=MsaT040894.1.exon20;Parent=MsaT040894.1
Chr7 CDS 104952829 104952915 104952829 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104953021 104953110 104953021 ID=MsaT040894.1.exon19;Parent=MsaT040894.1
Chr7 CDS 104953021 104953110 104953021 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104953302 104953349 104953302 ID=MsaT040894.1.exon18;Parent=MsaT040894.1
Chr7 CDS 104953302 104953349 104953302 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104953449 104953532 104953449 ID=MsaT040894.1.exon17;Parent=MsaT040894.1
Chr7 CDS 104953449 104953532 104953449 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104953710 104953923 104953710 ID=MsaT040894.1.exon16;Parent=MsaT040894.1
Chr7 CDS 104953710 104953923 104953710 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104954011 104954075 104954011 ID=MsaT040894.1.exon15;Parent=MsaT040894.1
Chr7 CDS 104954011 104954075 104954011 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104954284 104954388 104954284 ID=MsaT040894.1.exon14;Parent=MsaT040894.1
Chr7 CDS 104954284 104954388 104954284 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104954720 104954865 104954720 ID=MsaT040894.1.exon13;Parent=MsaT040894.1
Chr7 CDS 104954720 104954865 104954720 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104955364 104955488 104955364 ID=MsaT040894.1.exon12;Parent=MsaT040894.1
Chr7 CDS 104955364 104955488 104955364 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104955637 104955736 104955637 ID=MsaT040894.1.exon11;Parent=MsaT040894.1
Chr7 CDS 104955637 104955736 104955637 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104955890 104955962 104955890 ID=MsaT040894.1.exon10;Parent=MsaT040894.1
Chr7 CDS 104955890 104955962 104955890 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104956184 104956319 104956184 ID=MsaT040894.1.exon9;Parent=MsaT040894.1
Chr7 CDS 104956184 104956319 104956184 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104956705 104956797 104956705 ID=MsaT040894.1.exon8;Parent=MsaT040894.1
Chr7 CDS 104956705 104956797 104956705 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104956903 104956994 104956903 ID=MsaT040894.1.exon7;Parent=MsaT040894.1
Chr7 CDS 104956903 104956994 104956903 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104957108 104957176 104957108 ID=MsaT040894.1.exon6;Parent=MsaT040894.1
Chr7 CDS 104957108 104957176 104957108 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104957278 104957346 104957278 ID=MsaT040894.1.exon5;Parent=MsaT040894.1
Chr7 CDS 104957278 104957346 104957278 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104957445 104957552 104957445 ID=MsaT040894.1.exon4;Parent=MsaT040894.1
Chr7 CDS 104957445 104957552 104957445 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104958066 104958234 104958066 ID=MsaT040894.1.exon3;Parent=MsaT040894.1
Chr7 CDS 104958066 104958234 104958066 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104958325 104958380 104958325 ID=MsaT040894.1.exon2;Parent=MsaT040894.1
Chr7 CDS 104958325 104958380 104958325 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Chr7 exon 104958486 104959190 104958486 ID=MsaT040894.1.exon1;Parent=MsaT040894.1
Chr7 CDS 104958486 104959190 104958486 ID=cds.MsaT040894.1;Parent=MsaT040894.1
Gene Sequence

>MsaT040894.1

ATGAAATCTGGAATTCAGAATCTGAAGCGATGTAGTAAGCTAGAAGAAGAATCTCCATCACAGATTCAGAAGAAGCCCAAACTCAATAACGCGTTTTACTCTGTCCGTGATGGCGAGATCCACTCCAATTCAGCTTCCACCGATTTGAATATTGTAGCTGAAAATGCACCTCCATTGTCGCGATCATCCAGAGGCCGCGTTAAAATGCTTCCATCCAAGTTCAAGGACTCTGTACTTCTCGGTACTCAGAAGAAGAAGAAGAAGAGTCAACAAATCAAAAGATCTAAGCTCGGTTTTGAAGGTGAGAATAGTGACATTGGAATTAGATTTGTAAATAAGGGAAAGGAAAACAAATGCGATTTGAAAAAAGCGATTGATAATGGTTTTGATGAATTTGATTATGAGAAGCATGCTAAGTCGTTTAAGAGCATTAAAAAGACGGTTAAAGGGAGTCGTATGTTATTGGTTAAAGCGGAGAGTGAAAATGGTTTTGATGAAAAGGGTAAAAAGGGGAAAGAGATTTTCAAACTAGTGGATTTTCATTTGGGTGATATAGTTTGGGCAAAATGTGGCAAGACTTTTCCGGCATGGCCTGCTGTCGTCATTGATCCTATTTTGCAAGCTCCCGAGTCTGTTCTTAGGCGCTGTGTTCCCGATGCAATTTGTGTTATGTTTTTCGGTTATTCAAAAAATGGAAAGCAAAGGGACTATGCGTGGGCGAAGCAGGGGATGGTATTCCCCTTCTTGGAATTCATGGATAGATTCCAGGGGCATACCCGATTGTACAAGACCAAAGAAAGTGACTTTCATAAGGCAATGGAGGAGGCAATGTTGGCAGATAATGGCATTCTGGATCCACATTATAGAGCTGAGCAAATTGTGGAGGGTGCAACTTCCTGTGCTGAACAGGAATATCACTGCCAAGGCCAGGATACAATATCTTGTTATGGCTGCGGTTTGACGTTGCCATGCAAAACCATGAAGAAGATTAAGGATTCAAGCTGTGCTCCTCAGCATTATTGTAAATCTTGTGCAAAGTTACTTAAATCGAAACAATACTGTGGCATTTGCAAAAAGATTTGGCACCATTCTGATGCTGGTGATTGGGTATGCTGTGATGGTTGTAATGTTTGGGTGCATGCTGAGTGTGACAAAATTTCCAGCAAACGTTTCAAGGATCTGGAAAATATAGATTACTATTGCCCAGATTGCAAGGGAAAGTCGGATCGTAAATTATCCACATCACAAACATACAAATCAAAAATCAAATCAGTAGAAAACAACCAAAGACCTGTGTTGCCTGAAAATCTAGCGGTGGTGTGCAATGGCTTGAAAGGAATATACTTTCCAAAGCTTCATTTAGTTTTGTGCAAGTGTAGCACCTGTGGGTCAAAAAAGCAGGCATTGGCTGAATGGGAACGGCATACAGGTTGTACAGCCAAAAAATGGAAACATAGTGTGAAAGTAGAAGGCACAATGCAACCACTAATAAAATGGATCACGGAACACAATCCCCAAGCTGGAATTTCCATGAAGTTAAGTCAGAAACAGGTGTTTTCCTTTTTGCAAGAAAAATATGAACCAGTTTATGCAAAATGGACAACAGAGAGATGTGCGGTTTGCAGATGGGTAGAAGATTGGGAAGATAACAAGATTATTATATGTAACAGATGCCAAGTTGCTGTTCACCAAGAGTGCTATGGTGCAAAGAATCTCCAAGATTTTACTTCCTGGGTTTGTAGAGTGTGTGAAACTCCTGATGTAGAAAGAGAGTGTTGCCTCTGTCCAGTAAAAGGTGGTGCACTGAAGCCAACTGATGTTGAGTTGCTGTGGGTTCATGTAACATGTGCTTGGTTCCGGCCTGAAGTTGTTTTCCGAAACCATGAGGCAATGGAACCTGCCTCTGGAATCCTTAAGATTGCTCCTAGTTCCTTTTTGAAGACTTGTGTGATCTGTAAACAAAGCCATGGTTCCTGTACAAGTTGTTGCAAGTGTGCTACATATTTTCATGTGATGTGTGCATCAAGGATGGGATATAGCATGGCATTGCGTTCTACAGTGAAGAAGGGGACTCAAATAACGCAGACGCTAATATATTGTGCAGTTCACAGGATCCCAAATCCAGATTCAGTAATGGTTGTGCATACCCCTCTGGGGGTATTCTCTCCAAGAACTTCACTTCAAAATCTCAAGGGACGCTTTAGAGGATCAAAGCTTATTTCATCAAATAACTTAGAACTCCTTGAACCTTCAACCTCTGAACATTATGAGGTTGAGCCACTATCTGCCGCCAAGTGTCGTGTTTACAAAAGGCCTCCCAATAAGAAGGATGATGCACCGATAATTCACTTGCTTGGGGGACCAAACCTTCATTCTTTAAGTGCAATAACTCAATTGAACAACAATACGGATGCCGGGGTGTTTTCTTCATTTAAGGAACGCCTTCACCATTTACAGAAAACGGAAAATTGTAGAGTCTGCCTTGGAAAATCAGCTATTCAAGGGTGGGGTCTCTTTGCTCGTAGAGATTTGCAAGAAGGGGACATGGTTGTTGAGTATCGTGGTGAACAGTTAAGGCGTAGTGTTGCTGATCTGAGGGAAGCAAAGTACCGGTTAGAAGGCAAAGATTGTTATCTGTTCAAGATTAGTGAGGAAGTGGTAATTGATGCAACTGATAAGGGCAATATTGCACGTCTAATAAATCATTCTTGCATGCCAAACTGCTTTGCAAGGATTATGTGCTTGGGAGATCAGGAGAGCAGGATTGTTCTTATTGCCAAGACCAATATTTCTGCTGGTGAAGAACTAACGTATGATTACTTGTTCGATACAGATGAGCGAGATGAACTCAAGGTTCCCTGCCACTGCAAAGCCCCCAATTGTAGGAAATTTATGAACTAG
Protein sequence

>MsaT040894.1

MKSGIQNLKRCSKLEEESPSQIQKKPKLNNAFYSVRDGEIHSNSASTDLNIVAENAPPLSRSSRGRVKMLPSKFKDSVLLGTQKKKKKSQQIKRSKLGFEGENSDIGIRFVNKGKENKCDLKKAIDNGFDEFDYEKHAKSFKSIKKTVKGSRMLLVKAESENGFDEKGKKGKEIFKLVDFHLGDIVWAKCGKTFPAWPAVVIDPILQAPESVLRRCVPDAICVMFFGYSKNGKQRDYAWAKQGMVFPFLEFMDRFQGHTRLYKTKESDFHKAMEEAMLADNGILDPHYRAEQIVEGATSCAEQEYHCQGQDTISCYGCGLTLPCKTMKKIKDSSCAPQHYCKSCAKLLKSKQYCGICKKIWHHSDAGDWVCCDGCNVWVHAECDKISSKRFKDLENIDYYCPDCKGKSDRKLSTSQTYKSKIKSVENNQRPVLPENLAVVCNGLKGIYFPKLHLVLCKCSTCGSKKQALAEWERHTGCTAKKWKHSVKVEGTMQPLIKWITEHNPQAGISMKLSQKQVFSFLQEKYEPVYAKWTTERCAVCRWVEDWEDNKIIICNRCQVAVHQECYGAKNLQDFTSWVCRVCETPDVERECCLCPVKGGALKPTDVELLWVHVTCAWFRPEVVFRNHEAMEPASGILKIAPSSFLKTCVICKQSHGSCTSCCKCATYFHVMCASRMGYSMALRSTVKKGTQITQTLIYCAVHRIPNPDSVMVVHTPLGVFSPRTSLQNLKGRFRGSKLISSNNLELLEPSTSEHYEVEPLSAAKCRVYKRPPNKKDDAPIIHLLGGPNLHSLSAITQLNNNTDAGVFSSFKERLHHLQKTENCRVCLGKSAIQGWGLFARRDLQEGDMVVEYRGEQLRRSVADLREAKYRLEGKDCYLFKISEEVVIDATDKGNIARLINHSCMPNCFARIMCLGDQESRIVLIAKTNISAGEELTYDYLFDTDERDELKVPCHCKAPNCRKFMN*