AlfalfaGEDB | MsaG040998

M. sativa ssp. caerulea / MsaG040998

KEGG: -
GO: GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141
PFAM: PF03106.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG040998	XP_003626678.1	80.000	300	32	6	5	299	3	279	9.26e-155	443

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG040998	sp\|Q9LY00\|WRK70_ARATH	38.674	181	83	7	6	168	10	180	2.54e-28	113

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG040998	tr\|G7LAR2\|G7LAR2_MEDTR	80.000	300	32	6	5	299	3	279	4.42e-155	443

TFs/TRs:

Gene ID	Type	Classification
MsaG040998	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG040998	MsaG041512	0.882865	1.552767e-70	3.208476e-67

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG040998	MtrunA17_Chr8g0334541	80.000	300	32	6	5	299	3	279	8.51e-159	443
MsaG040998	MtrunA17_Chr8g0350621	48.473	262	104	9	1	250	1	243	1.89e-67	211
MsaG040998	MtrunA17_Chr7g0244971	43.836	292	138	8	4	284	12	288	3.71e-60	193
MsaG040998	MtrunA17_Chr7g0244981	40.476	294	135	9	4	284	11	277	4.87e-58	187
MsaG040998	MtrunA17_Chr2g0315371	39.111	225	97	8	9	201	18	234	4.80e-39	139
MsaG040998	MtrunA17_Chr8g0350631	49.419	172	59	6	128	297	4	149	1.50e-37	130
MsaG040998	MtrunA17_Chr2g0315381	38.247	251	104	10	2	210	10	251	4.57e-34	126
MsaG040998	MtrunA17_Chr2g0315361	39.095	243	102	9	6	209	14	249	7.06e-33	122
MsaG040998	MtrunA17_Chr3g0128671	45.238	168	75	7	13	167	23	186	1.57e-29	114
MsaG040998	MtrunA17_Chr5g0428351	40.000	165	82	4	8	168	92	243	1.48e-28	111
MsaG040998	MtrunA17_Chr8g0350641	45.588	136	62	4	6	132	7	139	2.54e-25	98.6
MsaG040998	MtrunA17_Chr7g0275531	35.795	176	94	6	4	167	9	177	3.51e-22	94.7
MsaG040998	MtrunA17_Chr3g0126391	29.412	204	109	5	1	174	1	199	7.78e-19	85.5
MsaG040998	MtrunA17_Chr8g0387981	30.729	192	102	5	8	174	13	198	9.03e-18	82.4
MsaG040998	MtrunA17_Chr8g0347251	45.349	86	36	2	93	167	110	195	1.40e-15	76.3
MsaG040998	MtrunA17_Chr2g0315401	52.542	59	27	1	136	194	19	76	4.19e-11	60.1
MsaG040998	MtrunA17_Chr7g0248721	50.000	64	24	3	112	170	141	201	8.92e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG040998	AT3G56400.1	38.674	181	83	7	6	168	10	180	2.58e-29	113
MsaG040998	AT2G46400.1	33.854	192	111	5	9	196	6	185	6.97e-23	96.3
MsaG040998	AT1G66550.1	31.068	206	108	7	6	199	8	191	4.13e-22	93.2
MsaG040998	AT1G66550.2	30.622	209	100	6	6	199	8	186	3.84e-21	90.5
MsaG040998	AT5G22570.1	31.188	202	115	6	5	193	8	198	6.04e-21	90.9
MsaG040998	AT1G66600.1	31.795	195	108	5	6	195	8	182	1.05e-20	89.4
MsaG040998	AT1G66560.1	34.010	197	99	8	7	195	9	182	5.66e-20	87.4
MsaG040998	AT4G11070.1	29.612	206	96	5	3	169	7	202	6.71e-18	82.8
MsaG040998	AT2G40750.1	29.487	234	125	7	6	203	14	243	6.78e-18	83.2
MsaG040998	AT5G01900.1	34.682	173	96	6	4	167	5	169	6.45e-17	79.3
MsaG040998	AT5G24110.1	30.337	178	103	5	4	173	14	178	2.50e-15	75.5
MsaG040998	AT4G11070.2	30.270	185	90	4	3	169	7	170	2.83e-15	74.7
MsaG040998	AT2G40740.1	35.032	157	51	6	89	226	137	261	3.67e-15	74.7
MsaG040998	AT2G40740.3	35.032	157	51	6	89	226	146	270	4.22e-15	74.7
MsaG040998	AT1G80590.1	30.723	166	82	5	6	167	8	144	4.84e-15	73.6
MsaG040998	AT4G23810.1	44.318	88	38	2	93	169	132	219	2.77e-14	72.4
MsaG040998	AT2G46130.2	47.541	61	27	2	112	170	30	87	2.38e-11	60.1

Find 59 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATATACACACCTTGAGTATT+TGG	0.203884	8:-413986	None:intergenic
TGATGTCGACGATGATAAAA+AGG	0.241439	8:-414371	None:intergenic
GAAATAGAGTTATCTAAAAT+TGG	0.244498	8:-414484	None:intergenic
ACAAGATGTTGTGCAAATAA+TGG	0.269160	8:-413416	None:intergenic
TTCAATTCTCTGCACTTGTT+TGG	0.311204	8:-414188	None:intergenic
CAAGATGTTGTGCAAATAAT+GGG	0.321057	8:-413415	None:intergenic
CAAGGAGAACTCACCTTCCT+TGG	0.368416	8:+414438	MsaT040998.1:CDS
TTTGATGATGTTTGTTCAAA+AGG	0.368943	8:-413475	None:intergenic
ACAAGTGTGGTGGCCGTAAT+AGG	0.383562	8:-414236	None:intergenic
ACCTTGAGTATTTGGAATTG+AGG	0.393271	8:-413978	None:intergenic
GAGTTATCTAAAATTGGTTC+AGG	0.405651	8:-414478	None:intergenic
TATAATTGAAAGCCATGAAA+AGG	0.418677	8:-414634	None:intergenic
CAATCTTGTTCGGTTGGAGT+TGG	0.418906	8:-414346	None:intergenic
CAACAGATGATGGGCATGAA+TGG	0.421200	8:+413932	MsaT040998.1:CDS
TTGTATAAGACCACCTATTA+CGG	0.432341	8:+414223	MsaT040998.1:CDS
GTTGTTAAAGCTAAGCAATA+TGG	0.446307	8:-414317	None:intergenic
GGGCATGAATGGAGAAAGTA+TGG	0.453906	8:+413943	MsaT040998.1:CDS
TCAGAAAGACCAACAGATGA+TGG	0.460980	8:+413922	MsaT040998.1:CDS
AATCTTGTTCGGTTGGAGTT+GGG	0.465300	8:-414345	None:intergenic
CACTTGAATCTGATGATCAC+AGG	0.481025	8:+414545	MsaT040998.1:CDS
TTTGAACAAACATCATCAAA+GGG	0.495098	8:+413478	MsaT040998.1:CDS
TTTAACTATTAAATCTGAAG+AGG	0.496351	8:+413514	MsaT040998.1:CDS
CATTCATGCCCATCATCTGT+TGG	0.504398	8:-413931	None:intergenic
TGATAGAGATGAGGCAATGA+AGG	0.505201	8:+413313	MsaT040998.1:CDS
AAGGGACAATCTTGTTCGGT+TGG	0.505479	8:-414352	None:intergenic
AGTATTATCATGAGGAGAAG+TGG	0.509303	8:-414287	None:intergenic
GATATGATGATAATATTGTA+TGG	0.516217	8:+414568	MsaT040998.1:CDS
ATAATTGAAAGCCATGAAAA+GGG	0.537651	8:-414633	None:intergenic
CTAGAGGATCTCACAAAAGA+AGG	0.538260	8:+413576	MsaT040998.1:CDS
GATGTTTGTTCAAAAGGAAG+AGG	0.548943	8:-413469	None:intergenic
GTGAGATCCTCTAGCACCAC+GGG	0.549156	8:-413567	None:intergenic
TAGATAACTCTATTTCTACA+AGG	0.554534	8:+414491	MsaT040998.1:CDS
TAATAGGTGGTCTTATACAA+GGG	0.559513	8:-414220	None:intergenic
TCCTCAATTCCAAATACTCA+AGG	0.566315	8:+413977	MsaT040998.1:CDS
GTATTATCATGAGGAGAAGT+GGG	0.567061	8:-414286	None:intergenic
TTGTTCGGTTGGAGTTGGGA+AGG	0.567594	8:-414341	None:intergenic
CAGAAAGACCAACAGATGAT+GGG	0.573763	8:+413923	MsaT040998.1:CDS
ATGTTTGTTCAAAAGGAAGA+GGG	0.576811	8:-413468	None:intergenic
GTAATAGGTGGTCTTATACA+AGG	0.578341	8:-414221	None:intergenic
AGTTCCACCCGTGGTGCTAG+AGG	0.580528	8:+413560	MsaT040998.1:CDS
AGAGAGCAGGTATTCATCCA+AGG	0.581141	8:-414455	None:intergenic
GAAAGCCATGAAAAGGGTGA+AGG	0.584427	8:-414627	None:intergenic
GCAATATTCCACTGAATGTG+AGG	0.603090	8:-414416	None:intergenic
AGCAGGTATTCATCCAAGGA+AGG	0.605278	8:-414451	None:intergenic
ACATTCAGTGGAATATTGCA+AGG	0.616091	8:+414420	MsaT040998.1:CDS
GTTAAAGCTAAGCAATATGG+AGG	0.618068	8:-414314	None:intergenic
GAGGAAGTAGTATTATCATG+AGG	0.627255	8:-414295	None:intergenic
AAATTGGTTCAGGAGAGAGC+AGG	0.628355	8:-414468	None:intergenic
GCAATGAAGGAGCTTGTGAG+AGG	0.633274	8:+413326	MsaT040998.1:CDS
CAATGAAGGAGCTTGTGAGA+GGG	0.635141	8:+413327	MsaT040998.1:CDS
CTAATTTGTTACAAGTGTGG+TGG	0.636211	8:-414246	None:intergenic
TGTGAGATCCTCTAGCACCA+CGG	0.642464	8:-413568	None:intergenic
AGATCCTCTAGCACCACGGG+TGG	0.645633	8:-413564	None:intergenic
AGTGTGGTGGCCGTAATAGG+TGG	0.645916	8:-414233	None:intergenic
AAATATGGATGATAGAGATG+AGG	0.650081	8:+413304	None:intergenic
ACTTGAATCTGATGATCACA+GGG	0.651483	8:+414546	MsaT040998.1:CDS
TGTCTAATTTGTTACAAGTG+TGG	0.656286	8:-414249	None:intergenic
TAGGTGGTCTTATACAAGGG+AGG	0.682870	8:-414217	None:intergenic
TACAAGACTAGTTCCACCCG+TGG	0.693459	8:+413551	MsaT040998.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	413308	414657	413308	ID=MsaG040998
Chr8	mRNA	413308	414657	413308	ID=MsaT040998.1;Parent=MsaG040998
Chr8	exon	413308	413597	413308	ID=MsaT040998.1.exon1;Parent=MsaT040998.1
Chr8	CDS	413308	413597	413308	ID=cds.MsaT040998.1;Parent=MsaT040998.1
Chr8	exon	413894	413998	413894	ID=MsaT040998.1.exon2;Parent=MsaT040998.1
Chr8	CDS	413894	413998	413894	ID=cds.MsaT040998.1;Parent=MsaT040998.1
Chr8	exon	414153	414657	414153	ID=MsaT040998.1.exon3;Parent=MsaT040998.1
Chr8	CDS	414153	414657	414153	ID=cds.MsaT040998.1;Parent=MsaT040998.1

Gene Sequence

>MsaT040998.1

ATGGATGATAGAGATGAGGCAATGAAGGAGCTTGTGAGAGGGCGTGAGTTAGCTAAGCAGCTTGAACAACATCTTAGTAACAACATTAATGATAAATTATTAGCAAGCCCATTATTTGCACAACATCTTGTAAGCAACGTTGTCAACTCATTTAGTAACACCCTCTTCCTTTTGAACAAACATCATCAAAGGGAACAAAACTTTTCTTTAACTATTAAATCTGAAGAGGAATCTCAAGATAGTTACAAGACTAGTTCCACCCGTGGTGCTAGAGGATCTCACAAAAGAAGAAAAACTACAGAAACGTGTGAGGAAGTGTCAGAAAGACCAACAGATGATGGGCATGAATGGAGAAAGTATGGTCAAAAAACAATCCTCAATTCCAAATACTCAAGGTGTACTCACAAGTTTAATCAAAGATGCAAAGCAACCAAACAAGTGCAGAGAATTGAAGAGAAGCCTCCCTTGTATAAGACCACCTATTACGGCCACCACACTTGTAACAAATTAGACAGTATCATATTTGAACCCACTTCTCCTCATGATAATACTACTTCCTCCATATTGCTTAGCTTTAACAACACCTTCCCAACTCCAACCGAACAAGATTGTCCCTTTTTATCATCGTCGACATCATCGTCATCTTCGTCTTTTAATTCCTCACATTCAGTGGAATATTGCAAGGAGAACTCACCTTCCTTGGATGAATACCTGCTCTCTCCTGAACCAATTTTAGATAACTCTATTTCTACAAGGCATGTTCGTAGTACTCTATCATCATCAACTACACTTGAATCTGATGATCACAGGGATATGATGATAATATTGTATGGTCTCTTGTATGATTCTGTTGAACTTGATACTGATTTCCTTCACCCTTTTCATGGCTTTCAATTATAG

Protein sequence

>MsaT040998.1

MDDRDEAMKELVRGRELAKQLEQHLSNNINDKLLASPLFAQHLVSNVVNSFSNTLFLLNKHHQREQNFSLTIKSEEESQDSYKTSSTRGARGSHKRRKTTETCEEVSERPTDDGHEWRKYGQKTILNSKYSRCTHKFNQRCKATKQVQRIEEKPPLYKTTYYGHHTCNKLDSIIFEPTSPHDNTTSSILLSFNNTFPTPTEQDCPFLSSSTSSSSSSFNSSHSVEYCKENSPSLDEYLLSPEPILDNSISTRHVRSTLSSSTTLESDDHRDMMIILYGLLYDSVELDTDFLHPFHGFQL*