AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041093

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041093 MtrunA17_Chr8g0335661 95.772 1088 44 2 1 1087 1 1087 0.0 2109
MsaG041093 MtrunA17_Chr4g0045841 29.091 165 95 5 905 1065 200 346 7.69e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041093 AT4G16970.1 54.183 526 214 8 555 1074 367 871 0.0 554

Find 248 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGTTCAGATCTCAGTTTC+AGG 0.190414 8:+1676186 MsaT041093.1:CDS
CGGAATATGCTTGAGCGTTT+TGG 0.199917 8:+1673321 MsaT041093.1:CDS
CTCTTCACTGCCCCGCAATT+TGG 0.205385 8:-1676385 None:intergenic
AGGCTCTAACATCCAATAAA+AGG 0.206842 8:+1674667 MsaT041093.1:CDS
AGTGAAACGGAGTTGAATTT+TGG 0.214768 8:-1670072 None:intergenic
ATGGATTGCTTTGACTCATT+TGG 0.234727 8:+1671534 MsaT041093.1:CDS
CATTCATCTACGGTTGCTTT+GGG 0.247411 8:+1675059 MsaT041093.1:CDS
ATTAAATCAAACAATGATTT+CGG 0.248376 8:-1677286 None:intergenic
TTAAATCAAACAATGATTTC+GGG 0.250887 8:-1677285 None:intergenic
AACATTATCAAATTTGTTAC+AGG 0.266909 8:-1672132 None:intergenic
TTTGAGTCTCGGATTGTAAA+TGG 0.273704 8:+1671207 MsaT041093.1:CDS
ACATTCATCTACGGTTGCTT+TGG 0.289832 8:+1675058 MsaT041093.1:CDS
TGGATTGCTTTGACTCATTT+GGG 0.302207 8:+1671535 MsaT041093.1:CDS
CTTTCTTCCTTTGAAAGGTA+TGG 0.302394 8:-1674895 None:intergenic
AACATTATCAGATTTGTTAC+AGG 0.302478 8:-1672357 None:intergenic
AGAAGATCCGACAGATGTTT+TGG 0.307148 8:+1671758 MsaT041093.1:CDS
AGAAGAGCCAACAAAAGTTT+TGG 0.311700 8:+1671917 MsaT041093.1:CDS
AGCAAATTGACTTGCGTTTA+CGG 0.311768 8:-1670096 None:intergenic
CGAGAGAAAAGGAAGTTTCC+AGG 0.319522 8:-1673985 None:intergenic
TATTCCTTGGGTTTACTGTT+AGG 0.322982 8:-1677318 None:intergenic
TAAATTCGCAACAGCCTTAT+CGG 0.327666 8:-1674781 None:intergenic
GATATTTCTTTAGGTGGTTA+TGG 0.330159 8:+1672743 MsaT041093.1:intron
GGGCATGCATGTATTAGATT+TGG 0.331939 8:+1671950 MsaT041093.1:CDS
TTCCTCCAAGATTCTTGTCT+TGG 0.332691 8:-1674641 None:intergenic
GAGCTACTTTATTGTTAGAC+TGG 0.342320 8:-1672229 None:intergenic
CTTTGAAAGGTATGGGTTCT+CGG 0.344019 8:-1674887 None:intergenic
TAATTAACCAATCCTTCCTT+TGG 0.344266 8:-1671678 None:intergenic
GTCCTTCTTCTCAGAGGAAA+AGG 0.345324 8:+1674976 MsaT041093.1:CDS
TGCGGGGCAGTGAAGAGCTT+TGG 0.350904 8:+1676391 MsaT041093.1:CDS
GCTTCCCTGTATTTGAAATT+CGG 0.353794 8:-1671291 None:intergenic
GAAGATTTCAGAAGCTGCTT+TGG 0.357370 8:-1672266 None:intergenic
AAAAGAAATTGCCAAATTGC+GGG 0.357929 8:+1676374 MsaT041093.1:CDS
GCGAGTTGCGGTGGTGGTTC+CGG 0.362514 8:-1669809 None:intergenic
GAAGGTTTCAGAAGCTGCTT+TGG 0.362927 8:-1672041 None:intergenic
TAGAAATCCGTTACTGTTAA+CGG 0.364241 8:-1669996 None:intergenic
AATGATGATGTTGTTGGTAT+TGG 0.365035 8:+1671498 MsaT041093.1:CDS
TCACCAATTGCAAGCCGTTT+CGG 0.366080 8:-1670241 None:intergenic
GATTCAATTGGAGAATGTTC+AGG 0.367519 8:+1671258 MsaT041093.1:CDS
CATTACACAAAGATTGATCT+TGG 0.372033 8:-1671479 None:intergenic
TATTGGATCAGAGGACAAAA+TGG 0.381016 8:+1671515 MsaT041093.1:CDS
CCTATACGTATTGATTCAAT+TGG 0.381191 8:+1671246 MsaT041093.1:CDS
CAGAACCGAATTTCAAATAC+AGG 0.382659 8:+1671286 MsaT041093.1:CDS
TTAACGGAGAGAGGAGAATC+AGG 0.386785 8:-1669980 None:intergenic
GCAAATTGACTTGCGTTTAC+GGG 0.391662 8:-1670095 None:intergenic
AGGGCCCTAAGGTTGATATT+TGG 0.397053 8:+1676206 MsaT041093.1:CDS
CTCTACTATACATGCTGATT+GGG 0.397374 8:+1676242 MsaT041093.1:CDS
GAAATCTTCTCGCATGTTGA+AGG 0.398448 8:+1672280 MsaT041093.1:CDS
CTTTGTGTGCAACTTGCACC+AGG 0.400113 8:-1677239 None:intergenic
TCAACACTAATCCTATTCCT+TGG 0.401290 8:-1677331 None:intergenic
TAACAGTAAACCCAAGGAAT+AGG 0.401457 8:+1677320 MsaT041093.1:CDS
ATAGAGGAAGAAGAAGGTTC+AGG 0.401686 8:+1672650 MsaT041093.1:CDS
CAACACTAATCCTATTCCTT+GGG 0.403336 8:-1677330 None:intergenic
TAAAGAGGAAAGAGGTTTGA+AGG 0.404692 8:+1671821 MsaT041093.1:CDS
GCGGGGCAGTGAAGAGCTTT+GGG 0.404708 8:+1676392 MsaT041093.1:CDS
CCAATTGAATCAATACGTAT+AGG 0.408213 8:-1671246 None:intergenic
AGATAACTTCATGACTTCTA+AGG 0.409633 8:+1672463 MsaT041093.1:CDS
AACTGTATTATAAAGTTTGA+AGG 0.410114 8:+1673432 MsaT041093.1:CDS
AGTGACTCGGTTAGAATTTG+AGG 0.413649 8:-1672107 None:intergenic
TTTCTTCCTTTGAAAGGTAT+GGG 0.416112 8:-1674894 None:intergenic
ACTAATGAAGAAACTCTCAT+TGG 0.417441 8:-1671373 None:intergenic
AAAAGGGTTACTGCTCCTTC+AGG 0.418092 8:+1674993 MsaT041093.1:CDS
GCAACGTGACGGACGAAATC+CGG 0.419646 8:-1669950 None:intergenic
TTCCTACGATCTGCCAGATC+TGG 0.424122 8:-1674696 None:intergenic
CAGTGAAGAGCTTTGGGAGG+TGG 0.427916 8:+1676398 MsaT041093.1:CDS
GCTCCTTCAGGCAAGGTAGA+TGG 0.430458 8:+1675005 MsaT041093.1:CDS
ACTCTACTATACATGCTGAT+TGG 0.431671 8:+1676241 MsaT041093.1:CDS
GATCCGAAACGGCTTGCAAT+TGG 0.437310 8:+1670238 MsaT041093.1:CDS
CCGCTTCTTCAACGCTTCGC+CGG 0.437967 8:+1669931 MsaT041093.1:CDS
CCCTACTGTGGATGGTGAAA+AGG 0.438053 8:+1671584 MsaT041093.1:CDS
AGACGGGAGAGGAATATTAC+TGG 0.438464 8:-1674601 None:intergenic
GAAACCTTCTCGCACATTGA+AGG 0.438686 8:+1672055 MsaT041093.1:CDS
AGTAATATTCCTCTCCCGTC+TGG 0.446156 8:+1674603 MsaT041093.1:CDS
TTCTCTCGAAAGCTAAAGAA+AGG 0.446434 8:+1674000 MsaT041093.1:CDS
GAGGAGAGAGAGGAGGTGCC+AGG 0.448642 8:-1669861 None:intergenic
CGTCGACGGGATGACCGATC+TGG 0.449155 8:-1669886 None:intergenic
ATTATAAAAGATTCAAAAGA+TGG 0.450944 8:-1672629 None:intergenic
TTGTGTAATGATGATGTTGT+TGG 0.453868 8:+1671492 MsaT041093.1:CDS
GCCGACCAAATATCAACCTT+AGG 0.456009 8:-1676211 None:intergenic
TATTCCGCTCATTATTTACA+TGG 0.456851 8:-1673305 None:intergenic
AAGGTTGATATTTGGTCGGC+TGG 0.458152 8:+1676214 MsaT041093.1:CDS
GTGGTCCAAGACAAGAATCT+TGG 0.458901 8:+1674636 MsaT041093.1:CDS
TTTGAAGGTTCTTTCAAAAG+TGG 0.458914 8:+1673447 MsaT041093.1:CDS
GTGCAATGAAATTTGAGTCT+CGG 0.462949 8:+1671196 MsaT041093.1:CDS
GGATCTTCTCCACATGCTAC+AGG 0.465432 8:-1671744 None:intergenic
GATTGATCTTGGACACTAGT+TGG 0.465730 8:-1671468 None:intergenic
AACAAAGGGTTTCCGTGCCC+CGG 0.466552 8:+1675227 MsaT041093.1:CDS
TAAAAGGGAATTGCCAGATC+TGG 0.468469 8:+1674683 MsaT041093.1:CDS
GGCTCTTCTCCACATACTAC+AGG 0.475497 8:-1671903 None:intergenic
CTTAGTGCTAGTAACATCTC+TGG 0.475547 8:-1674844 None:intergenic
TCCAATTCCAAAACATCTGT+CGG 0.478188 8:-1671765 None:intergenic
AACATTATTGAAGGTGATGA+AGG 0.478543 8:+1672185 MsaT041093.1:CDS
AATGATCAGAACATTATTGA+AGG 0.479243 8:+1672176 MsaT041093.1:CDS
AAATTCGCAACAGCCTTATC+GGG 0.479709 8:-1674780 None:intergenic
TTGTTCAGATCTCAGTTTCA+GGG 0.479868 8:+1676187 MsaT041093.1:CDS
GGCTCTAACATCCAATAAAA+GGG 0.480027 8:+1674668 MsaT041093.1:CDS
GGTCCATGTGTTGGAACAAA+GGG 0.481545 8:+1675213 MsaT041093.1:CDS
GCAACCGTAGATGAATGTAT+AGG 0.482776 8:-1675053 None:intergenic
CACTCCTCGAAGACCAATCC+TGG 0.482873 8:+1669843 MsaT041093.1:CDS
AGATTCTTGTCTTGGACCAC+AGG 0.483607 8:-1674633 None:intergenic
AGGATGCTTCAACGAGGACT+TGG 0.483684 8:+1677409 MsaT041093.1:CDS
AGCTCCTTTCTTCCTTTGAA+AGG 0.484570 8:-1674900 None:intergenic
CCGACCAAATATCAACCTTA+GGG 0.486366 8:-1676210 None:intergenic
CCACTGGAAAGGATGTTTCA+CGG 0.486521 8:-1676434 None:intergenic
ATTGGGAAAACTTCTTTCCC+TGG 0.486809 8:+1676259 MsaT041093.1:CDS
ATCTCAGTTTCAGGGCCCTA+AGG 0.486826 8:+1676195 MsaT041093.1:CDS
GAAAGATACTCCGCGTCGAC+GGG 0.487994 8:-1669899 None:intergenic
GGATCGGCGAGTTGCGGTGG+TGG 0.491104 8:-1669815 None:intergenic
TCTTTAGCTTTCGAGAGAAA+AGG 0.491702 8:-1673996 None:intergenic
GATGGAAGATGTTGCTCAAA+GGG 0.492219 8:+1671716 MsaT041093.1:CDS
GAGAGAGGAGGTGCCAGGAT+TGG 0.493624 8:-1669856 None:intergenic
AAGAGGTCCAAAGGAAGGAT+TGG 0.493708 8:+1671671 MsaT041093.1:CDS
CAAGAATCTTGGAGGAATCA+AGG 0.494455 8:+1674647 MsaT041093.1:CDS
TTTATAATAGAGGAAGAAGA+AGG 0.495240 8:+1672644 MsaT041093.1:CDS
TTACTGGATGCATCAAGCCT+TGG 0.496091 8:-1674585 None:intergenic
GAGGAAAGAGGTTTGAAGGA+AGG 0.496387 8:+1671825 MsaT041093.1:CDS
AGCACAAGGAGCAGATGGCT+CGG 0.498407 8:+1674809 MsaT041093.1:CDS
CTCTCTCTCCTCCTCCAGAT+CGG 0.499013 8:+1669872 MsaT041093.1:CDS
ATATGTGAGCTTCAGTGGTA+TGG 0.499209 8:+1673812 MsaT041093.1:CDS
AAACCTTCTCGCACATTGAA+GGG 0.499606 8:+1672056 MsaT041093.1:CDS
ATGGTCAAAGGAAAGATATC+TGG 0.502192 8:+1671336 MsaT041093.1:CDS
AAGCTGCTTTCGCCATGGCT+AGG 0.503048 8:+1670032 MsaT041093.1:CDS
GCACAAGGAGCAGATGGCTC+GGG 0.506807 8:+1674810 MsaT041093.1:CDS
TAAAAGAAATTGCCAAATTG+CGG 0.506980 8:+1676373 MsaT041093.1:CDS
CGTTGAAGCTGCTTTCGCCA+TGG 0.511259 8:+1670027 MsaT041093.1:CDS
TTGCGTTTACGGGAGTGAAA+CGG 0.511839 8:-1670085 None:intergenic
CATACCTTTCAAAGGAAGAA+AGG 0.513266 8:+1674896 MsaT041093.1:CDS
TCCTTCTTCTCAGAGGAAAA+GGG 0.519310 8:+1674977 MsaT041093.1:CDS
TTAGTGTTGAAGATGTTCTC+AGG 0.521851 8:+1677344 MsaT041093.1:CDS
AGCTGCTTTGGATTTGAAGA+GGG 0.525993 8:-1672254 None:intergenic
TATGTGAGCTTCAGTGGTAT+GGG 0.526504 8:+1673813 MsaT041093.1:CDS
TGAAATTCGTAAGCATATGA+AGG 0.528442 8:+1674725 MsaT041093.1:CDS
TATTGAAGGTGATGAAGGTA+AGG 0.529301 8:+1672190 MsaT041093.1:CDS
GTTCACAGAGATGTTAAACC+TGG 0.530796 8:+1673967 MsaT041093.1:CDS
AGCTACTTTATTGTTAGACT+GGG 0.530901 8:-1672228 None:intergenic
TGGTCTTCGAGGAGTGGAGT+TGG 0.531805 8:-1669836 None:intergenic
GATGGAAGATGTTGCTCTGA+GGG 0.533130 8:+1671875 MsaT041093.1:CDS
CATGGCGAAAGCAGCTTCAA+CGG 0.534287 8:-1670026 None:intergenic
AAACCCTTTGTTCCAACACA+TGG 0.534397 8:-1675216 None:intergenic
CATCAGTTGTTCTATGAGCA+CGG 0.536001 8:-1672775 None:intergenic
AGAAGAGAAAAGAGGTCCAA+AGG 0.536493 8:+1671662 MsaT041093.1:CDS
CGTGCTCATAGAACAACTGA+TGG 0.537080 8:+1672776 MsaT041093.1:CDS
TGGCAAGGTTGAAGTCGATA+AGG 0.538478 8:-1674026 None:intergenic
GAGAAAAGAGGTCCAAAGGA+AGG 0.538859 8:+1671666 MsaT041093.1:CDS
ATAAATAAAGAAGAGAAAAG+AGG 0.539398 8:+1671654 MsaT041093.1:CDS
TTGATATATGTGAGCTTCAG+TGG 0.539584 8:+1673807 MsaT041093.1:CDS
TGTTTCACGGTCATGCAGCT+TGG 0.540699 8:-1676421 None:intergenic
AACATAACCCCTACTGTGGA+TGG 0.541969 8:+1671576 MsaT041093.1:CDS
CCCTAAGGTTGATATTTGGT+CGG 0.545440 8:+1676210 MsaT041093.1:CDS
ATGAAGGCAAAAGCGGATGC+TGG 0.545882 8:+1674741 MsaT041093.1:CDS
CGATCTGGAGGAGGAGAGAG+AGG 0.549243 8:-1669871 None:intergenic
CCGGCGAAGCGTTGAAGAAG+CGG 0.552425 8:-1669931 None:intergenic
TTCGAGGAGTGGAGTTGGAT+CGG 0.552545 8:-1669831 None:intergenic
AAGCTGCTTTGGATTTGAAG+AGG 0.554463 8:-1672030 None:intergenic
GTAGATGAATGTATAGGCAT+TGG 0.557803 8:-1675047 None:intergenic
GGAAAGATACTCCGCGTCGA+CGG 0.560979 8:-1669900 None:intergenic
GGGTCCATGTGTTGGAACAA+AGG 0.561472 8:+1675212 MsaT041093.1:CDS
CGTTGAAGAAGCGGCAACGA+AGG 0.561497 8:-1669922 None:intergenic
AGATGGAAGATGTTGCTCAA+AGG 0.563134 8:+1671715 MsaT041093.1:CDS
AAGAATTTACCTCCGGGGCA+CGG 0.563272 8:-1675239 None:intergenic
AGGGTCAATCCTGTAGTATG+TGG 0.564548 8:+1671894 MsaT041093.1:CDS
AAGCTGCTTTGGATTTGAAG+AGG 0.565073 8:-1672255 None:intergenic
GATTCTTGTCTTGGACCACA+GGG 0.566206 8:-1674632 None:intergenic
CCTCTCTCCGTTAACAGTAA+CGG 0.568053 8:+1669989 MsaT041093.1:CDS
GGGGAAGAACCAGACGGGAG+AGG 0.568805 8:-1674612 None:intergenic
CGTAGAGCACAAGGAGCAGA+TGG 0.571518 8:+1674804 MsaT041093.1:CDS
TATGGGTTCTCGGAGCCTAT+CGG 0.573323 8:-1674877 None:intergenic
CTAAGACTGCTTCTGCCGAT+AGG 0.573679 8:+1674862 MsaT041093.1:CDS
TTATGAGAATGATAGAGAGA+TGG 0.574463 8:+1671698 MsaT041093.1:CDS
CCGTTACTGTTAACGGAGAG+AGG 0.575451 8:-1669989 None:intergenic
AAAAGAGAGGGTCCATGTGT+TGG 0.577815 8:+1675204 MsaT041093.1:CDS
GTCCAAGACAAGAATCTTGG+AGG 0.578441 8:+1674639 MsaT041093.1:CDS
CGGGATGACCGATCTGGAGG+AGG 0.579997 8:-1669880 None:intergenic
TCTTCTCGCATGTTGAAGGA+TGG 0.580399 8:+1672284 MsaT041093.1:CDS
TTGGGGTTATTAAGAAGCAA+AGG 0.581215 8:+1675077 MsaT041093.1:CDS
TTTGTTACAGGCTAGTGACT+CGG 0.581585 8:-1672120 None:intergenic
CTGCCATCTACCTTGCCTGA+AGG 0.582954 8:-1675008 None:intergenic
AAAGGAATTTACCACTGGAA+AGG 0.583882 8:-1676445 None:intergenic
TATCGACTTCAACCTTGCCA+TGG 0.583985 8:+1674029 MsaT041093.1:CDS
AACCTTCTCGCACATTGAAG+GGG 0.587830 8:+1672057 MsaT041093.1:CDS
CAGGATTGGTCTTCGAGGAG+TGG 0.587880 8:-1669842 None:intergenic
GTTGGATCGGCGAGTTGCGG+TGG 0.588249 8:-1669818 None:intergenic
CGAGGGTCTAGATAGATTCG+TGG 0.588972 8:+1671416 MsaT041093.1:CDS
TGGTGGAGCAAGATTAGCAA+AGG 0.589902 8:-1671448 None:intergenic
TGTCATATGATTGCAAGTAT+CGG 0.590214 8:-1677210 None:intergenic
TGATCTTGGACACTAGTTGG+TGG 0.590604 8:-1671465 None:intergenic
GCGAAAGCAGCTTCAACGGA+AGG 0.591262 8:-1670022 None:intergenic
CGAGAACCCATACCTTTCAA+AGG 0.593233 8:+1674888 MsaT041093.1:CDS
GCTAGTAACATCTCTGGCAG+AGG 0.593503 8:-1674838 None:intergenic
GGTGCCAGGATTGGTCTTCG+AGG 0.593716 8:-1669847 None:intergenic
CTTCAGGCAAGGTAGATGGC+AGG 0.594037 8:+1675009 MsaT041093.1:CDS
CAGGTGGAAGAGATCCGAAA+CGG 0.595991 8:+1670227 MsaT041093.1:intron
GGTGGAGCAAGATTAGCAAA+GGG 0.597178 8:-1671447 None:intergenic
AATGCCTATACATTCATCTA+CGG 0.599137 8:+1675049 MsaT041093.1:CDS
AGTCTCGGATTGTAAATGGT+GGG 0.602244 8:+1671211 MsaT041093.1:CDS
TCAATTTGCTCAATCACTCA+AGG 0.603232 8:+1670109 MsaT041093.1:CDS
GTTGAAGAAGCGGCAACGAA+GGG 0.603458 8:-1669921 None:intergenic
GGCATAACAAGCATGGTCAA+AGG 0.603701 8:+1671324 MsaT041093.1:CDS
CCACAGGGGAAGAACCAGAC+GGG 0.606761 8:-1674617 None:intergenic
ACCTTCTCGCACATTGAAGG+GGG 0.609586 8:+1672058 MsaT041093.1:CDS
GAGTCTCGGATTGTAAATGG+TGG 0.614229 8:+1671210 MsaT041093.1:CDS
TGCCAGATCTGGCAGATCGT+AGG 0.614643 8:+1674694 MsaT041093.1:CDS
GCGAATTTACGTAGAGCACA+AGG 0.616884 8:+1674795 MsaT041093.1:CDS
CCCGTCTGGTTCTTCCCCTG+TGG 0.618731 8:+1674617 MsaT041093.1:CDS
GGGCAGTGAAGAGCTTTGGG+AGG 0.619718 8:+1676395 MsaT041093.1:CDS
ATTCATCTACGGTTGCTTTG+GGG 0.621492 8:+1675060 MsaT041093.1:CDS
AGTTGCGGTGGTGGTTCCGG+CGG 0.621987 8:-1669806 None:intergenic
TAAGCATATGAAGGCAAAAG+CGG 0.635350 8:+1674734 MsaT041093.1:CDS
AGATGGAAGATGTTGCTCTG+AGG 0.636022 8:+1671874 MsaT041093.1:CDS
GGTTACTGCTCCTTCAGGCA+AGG 0.636110 8:+1674998 MsaT041093.1:CDS
TACAGGGAAGCTGATAATGA+CGG 0.636287 8:+1671303 MsaT041093.1:CDS
CGACGGGATGACCGATCTGG+AGG 0.637015 8:-1669883 None:intergenic
GGAGTTGGATCGGCGAGTTG+CGG 0.637588 8:-1669821 None:intergenic
TGAAAAGTCCTTCTTCTCAG+AGG 0.638414 8:+1674970 MsaT041093.1:CDS
TTTACTGTTCTGGTTCGCCA+GGG 0.640055 8:-1676276 None:intergenic
GACGGAAAACATAAAAGAGA+GGG 0.640908 8:+1675192 MsaT041093.1:CDS
GAGGGTCTAGATAGATTCGT+GGG 0.642595 8:+1671417 MsaT041093.1:CDS
ACTTGCGTGCTTGCACAAAG+AGG 0.643572 8:+1673853 MsaT041093.1:CDS
AGGGTCGATCCTGTAGCATG+TGG 0.645672 8:+1671735 MsaT041093.1:CDS
AAGTGACTCGAGGAAGCTCG+AGG 0.646512 8:+1671398 MsaT041093.1:CDS
AGAACCGAATTTCAAATACA+GGG 0.647420 8:+1671287 MsaT041093.1:CDS
TGTTGTTGGTATTGGATCAG+AGG 0.649031 8:+1671506 MsaT041093.1:CDS
AAAGAAATTGCCAAATTGCG+GGG 0.650726 8:+1676375 MsaT041093.1:CDS
AAAACCATGTAAATAATGAG+CGG 0.651500 8:+1673301 MsaT041093.1:CDS
TAATGACGGCATAACAAGCA+TGG 0.654148 8:+1671317 MsaT041093.1:CDS
GAAGCAACCTAACCATGGCA+AGG 0.654219 8:-1674041 None:intergenic
TGAAGGTAAGGATCAGAACA+TGG 0.659752 8:+1672202 MsaT041093.1:CDS
ACTCAGGTAAATCAAAACCA+AGG 0.660436 8:+1674568 MsaT041093.1:intron
AGTGCCTAACAGTAAACCCA+AGG 0.662467 8:+1677314 MsaT041093.1:CDS
AGTGACTCGAGGAAGCTCGA+GGG 0.663036 8:+1671399 MsaT041093.1:CDS
ACAAAACATAACCCCTACTG+TGG 0.663994 8:+1671572 MsaT041093.1:CDS
GGACGGAAAACATAAAAGAG+AGG 0.664528 8:+1675191 MsaT041093.1:CDS
CAGGAAGTGAAGCAACGTGA+CGG 0.666927 8:-1669961 None:intergenic
TTGAAGCAGAAGTACAACAT+CGG 0.674249 8:+1674141 MsaT041093.1:CDS
AATCATATGACATAGAAACC+TGG 0.675049 8:+1677221 MsaT041093.1:CDS
TCTGGAGGAGGAGAGAGAGG+AGG 0.676330 8:-1669868 None:intergenic
TCATTAGTGAAAGTGACTCG+AGG 0.676728 8:+1671388 MsaT041093.1:CDS
ATGAGATCATGAGAAAGACA+AGG 0.685426 8:+1677389 MsaT041093.1:CDS
AAAGGGTTTCCGTGCCCCGG+AGG 0.686493 8:+1675230 MsaT041093.1:CDS
ACCACAGGGGAAGAACCAGA+CGG 0.689092 8:-1674618 None:intergenic
TCTCATGATCTCATTACACG+AGG 0.690799 8:-1677380 None:intergenic
CCGTGAAACATCCTTTCCAG+TGG 0.691680 8:+1676434 MsaT041093.1:CDS
GATCGGTCATCCCGTCGACG+CGG 0.695572 8:+1669889 MsaT041093.1:CDS
AGCGCAAGAATTTACCTCCG+GGG 0.710001 8:-1675244 None:intergenic
CCTTCTCGCACATTGAAGGG+GGG 0.716670 8:+1672059 MsaT041093.1:CDS
TGTTGAGCATGAGAGACCTG+AGG 0.737768 8:+1673497 MsaT041093.1:CDS
ATTCTTGTCTTGGACCACAG+GGG 0.743915 8:-1674631 None:intergenic
AAGACAAGGATGCTTCAACG+AGG 0.751606 8:+1677403 MsaT041093.1:CDS
ATTGCATAGAATTCTGCACG+AGG 0.772903 8:-1674929 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 1669800 1677465 1669800 ID=MsaG041093
Chr8 mRNA 1669800 1677465 1669800 ID=MsaT041093.1;Parent=MsaG041093
Chr8 exon 1669800 1670130 1669800 ID=MsaT041093.1.exon1;Parent=MsaT041093.1
Chr8 CDS 1669800 1670130 1669800 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1670230 1670288 1670230 ID=MsaT041093.1.exon2;Parent=MsaT041093.1
Chr8 CDS 1670230 1670288 1670230 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1671150 1672484 1671150 ID=MsaT041093.1.exon3;Parent=MsaT041093.1
Chr8 CDS 1671150 1672484 1671150 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1672611 1672671 1672611 ID=MsaT041093.1.exon4;Parent=MsaT041093.1
Chr8 CDS 1672611 1672671 1672611 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1672756 1672818 1672756 ID=MsaT041093.1.exon5;Parent=MsaT041093.1
Chr8 CDS 1672756 1672818 1672756 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1673280 1673343 1673280 ID=MsaT041093.1.exon6;Parent=MsaT041093.1
Chr8 CDS 1673280 1673343 1673280 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1673425 1673518 1673425 ID=MsaT041093.1.exon7;Parent=MsaT041093.1
Chr8 CDS 1673425 1673518 1673425 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1673791 1673874 1673791 ID=MsaT041093.1.exon8;Parent=MsaT041093.1
Chr8 CDS 1673791 1673874 1673791 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1673961 1674050 1673961 ID=MsaT041093.1.exon9;Parent=MsaT041093.1
Chr8 CDS 1673961 1674050 1673961 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1674138 1674165 1674138 ID=MsaT041093.1.exon10;Parent=MsaT041093.1
Chr8 CDS 1674138 1674165 1674138 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1674574 1675098 1674574 ID=MsaT041093.1.exon11;Parent=MsaT041093.1
Chr8 CDS 1674574 1675098 1674574 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1675190 1675251 1675190 ID=MsaT041093.1.exon12;Parent=MsaT041093.1
Chr8 CDS 1675190 1675251 1675190 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1676184 1676293 1676184 ID=MsaT041093.1.exon13;Parent=MsaT041093.1
Chr8 CDS 1676184 1676293 1676184 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1676368 1676455 1676368 ID=MsaT041093.1.exon14;Parent=MsaT041093.1
Chr8 CDS 1676368 1676455 1676368 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Chr8 exon 1677196 1677465 1677196 ID=MsaT041093.1.exon15;Parent=MsaT041093.1
Chr8 CDS 1677196 1677465 1677196 ID=cds.MsaT041093.1;Parent=MsaT041093.1
Gene Sequence

>MsaT041093.1

ATGTCTCCGCCGGAACCACCACCGCAACTCGCCGATCCAACTCCACTCCTCGAAGACCAATCCTGGCACCTCCTCTCTCTCCTCCTCCAGATCGGTCATCCCGTCGACGCGGAGTATCTTTCCCTTCGTTGCCGCTTCTTCAACGCTTCGCCGGATTTCGTCCGTCACGTTGCTTCACTTCCTGATTCTCCTCTCTCCGTTAACAGTAACGGATTTCTAACTCCTTCCGTTGAAGCTGCTTTCGCCATGGCTAGGTTTTTCTCATTTCAGTTCCAAAATTCAACTCCGTTTCACTCCCGTAAACGCAAGTCAATTTGCTCAATCACTCAAGGTGGAAGAGATCCGAAACGGCTTGCAATTGGTGATAAAGTTCCAAAAATTTTCGTTAAGAGTTTTGCTGATACTACTGCAGAGGCTGTTATGAGAAGAAGTTTCTGTGCAATGAAATTTGAGTCTCGGATTGTAAATGGTGGGAATTATATGATTCCTATACGTATTGATTCAATTGGAGAATGTTCAGGTTGTTCAGAACCGAATTTCAAATACAGGGAAGCTGATAATGACGGCATAACAAGCATGGTCAAAGGAAAGATATCTGGCTCTCTTATCAAAGCCAATGAGAGTTTCTTCATTAGTGAAAGTGACTCGAGGAAGCTCGAGGGTCTAGATAGATTCGTGGGACATAATCCCTTTGCTAATCTTGCTCCACCAACTAGTGTCCAAGATCAATCTTTGTGTAATGATGATGTTGTTGGTATTGGATCAGAGGACAAAATGGATTGCTTTGACTCATTTGGGAATGAATATCCTGAACAAAACATAACCCCTACTGTGGATGGTGAAAAGGTTTGTCGAAATAATACTTGCAGAGATTCACTACTAGAATCTAATGAAATAAATAAAGAAGAGAAAAGAGGTCCAAAGGAAGGATTGGTTAATTATGAGAATGATAGAGAGATGGAAGATGTTGCTCAAAGGGTCGATCCTGTAGCATGTGGAGAAGATCCGACAGATGTTTTGGAATTGGAAAAGGGTATAATAAGAGAATCTATTGAAAAATATAAAGAGGAAAGAGGTTTGAAGGAAGGAATGATTAACTATGACAAAAATGAAGAGATGGAAGATGTTGCTCTGAGGGTCAATCCTGTAGTATGTGGAGAAGAGCCAACAAAAGTTTTGGAATTGAAAAAGGGCATGCATGTATTAGATTTGGATACAAACAAGACAGTGAGAAAAATGGTGACCCGGTCTACAAATAAAATAGCTCAGTCCTCTTCAAATCCAAAGCAGCTTCTGAAACCTTCTCGCACATTGAAGGGGGGACAGAAAAATGATCTACACTCTAAGCCTCAAATTCTAACCGAGTCACTAGCCTGTAACAAATTTGATAATGTTCCAAAAAAAATTGATCAAAGTAATGATCAGAACATTATTGAAGGTGATGAAGGTAAGGATCAGAACATGGTGACCCAGTCTAACAATAAAGTAGCTCAGCCCTCTTCAAATCCAAAGCAGCTTCTGAAATCTTCTCGCATGTTGAAGGATGGACAGAAACATGATCTACACTCAAAGACTCAAAATCTAACTGAGTCACTAGCCTGTAACAAATCTGATAATGTTCCAAAAAAAATTGATCAAGGTCATGATCAGAGTATTATTTCAAAAAACAAAATAAAACAAAATCGTAAAGAAAACGCGACAGAAGATAACTTCATGACTTCTAAGGTAGAGAAAAAAACATTTCCATCTTTTGAATCTTTTATAATAGAGGAAGAAGAAGGTTCAGGTGGTTATGGCATTGTTTACCGTGCTCATAGAACAACTGATGGAAAAAGACTTGCTATAAAATGTCCTCATAGCAATGCTCATAAAAACCATGTAAATAATGAGCGGAATATGCTTGAGCGTTTTGGGGGTAAAAACTGTATTATAAAGTTTGAAGGTTCTTTCAAAAGTGGCAACAGTGACTGCTTTGTTTTAGAACATGTTGAGCATGAGAGACCTGAGGTTTTGAAAAAAGAAATTGATATATGTGAGCTTCAGTGGTATGGGTTTTGCATGTTCAAGGCACTTGCGTGCTTGCACAAAGAGGGAGTTGTTCACAGAGATGTTAAACCTGGAAACTTCCTTTTCTCTCGAAAGCTAAAGAAAGGCTACCTTATCGACTTCAACCTTGCCATGGATTTGAAGCAGAAGTACAACATCGGAAGTAAATCAAAACCAAGGCTTGATGCATCCAGTAATATTCCTCTCCCGTCTGGTTCTTCCCCTGTGGTCCAAGACAAGAATCTTGGAGGAATCAAGGCTCTAACATCCAATAAAAGGGAATTGCCAGATCTGGCAGATCGTAGGAAATATTATGAAATTCGTAAGCATATGAAGGCAAAAGCGGATGCTGGTCATCTGAAAAATTGTCCCGATAAGGCTGTTGCGAATTTACGTAGAGCACAAGGAGCAGATGGCTCGGGTATAACCTCTGCCAGAGATGTTACTAGCACTAAGACTGCTTCTGCCGATAGGCTCCGAGAACCCATACCTTTCAAAGGAAGAAAGGAGCTTATCAGCCTCGTGCAGAATTCTATGCAATGTGCAAACAATAGTTCAATGAAAAGTCCTTCTTCTCAGAGGAAAAGGGTTACTGCTCCTTCAGGCAAGGTAGATGGCAGGACGCTTTATCTTACGCCAATGCCTATACATTCATCTACGGTTGCTTTGGGGTTATTAAGAAGCAAAGGGGACGGAAAACATAAAAGAGAGGGTCCATGTGTTGGAACAAAGGGTTTCCGTGCCCCGGAGGTTTTGTTCAGATCTCAGTTTCAGGGCCCTAAGGTTGATATTTGGTCGGCTGGAGTCACTCTACTATACATGCTGATTGGGAAAACTTCTTTCCCTGGCGAACCAGAACAGAGTTTAAAAGAAATTGCCAAATTGCGGGGCAGTGAAGAGCTTTGGGAGGTGGCCAAGCTGCATGACCGTGAAACATCCTTTCCAGTGGAGTTATTTGATGACCGATACTTGCAATCATATGACATAGAAACCTGGTGCAAGTTGCACACAAAGAGACCAGAGTTTGTTGATAAAATCCCGAAATCATTGTTTGATTTAATAGAGAAGTGCCTAACAGTAAACCCAAGGAATAGGATTAGTGTTGAAGATGTTCTCAGGCATGAGTTTTTTGCCTCGTGTAATGAGATCATGAGAAAGACAAGGATGCTTCAACGAGGACTTGGCTTAGAAACTGCAGCAGATTCAAGAGCAGTCTGA
Protein sequence

>MsaT041093.1

MSPPEPPPQLADPTPLLEDQSWHLLSLLLQIGHPVDAEYLSLRCRFFNASPDFVRHVASLPDSPLSVNSNGFLTPSVEAAFAMARFFSFQFQNSTPFHSRKRKSICSITQGGRDPKRLAIGDKVPKIFVKSFADTTAEAVMRRSFCAMKFESRIVNGGNYMIPIRIDSIGECSGCSEPNFKYREADNDGITSMVKGKISGSLIKANESFFISESDSRKLEGLDRFVGHNPFANLAPPTSVQDQSLCNDDVVGIGSEDKMDCFDSFGNEYPEQNITPTVDGEKVCRNNTCRDSLLESNEINKEEKRGPKEGLVNYENDREMEDVAQRVDPVACGEDPTDVLELEKGIIRESIEKYKEERGLKEGMINYDKNEEMEDVALRVNPVVCGEEPTKVLELKKGMHVLDLDTNKTVRKMVTRSTNKIAQSSSNPKQLLKPSRTLKGGQKNDLHSKPQILTESLACNKFDNVPKKIDQSNDQNIIEGDEGKDQNMVTQSNNKVAQPSSNPKQLLKSSRMLKDGQKHDLHSKTQNLTESLACNKSDNVPKKIDQGHDQSIISKNKIKQNRKENATEDNFMTSKVEKKTFPSFESFIIEEEEGSGGYGIVYRAHRTTDGKRLAIKCPHSNAHKNHVNNERNMLERFGGKNCIIKFEGSFKSGNSDCFVLEHVEHERPEVLKKEIDICELQWYGFCMFKALACLHKEGVVHRDVKPGNFLFSRKLKKGYLIDFNLAMDLKQKYNIGSKSKPRLDASSNIPLPSGSSPVVQDKNLGGIKALTSNKRELPDLADRRKYYEIRKHMKAKADAGHLKNCPDKAVANLRRAQGADGSGITSARDVTSTKTASADRLREPIPFKGRKELISLVQNSMQCANNSSMKSPSSQRKRVTAPSGKVDGRTLYLTPMPIHSSTVALGLLRSKGDGKHKREGPCVGTKGFRAPEVLFRSQFQGPKVDIWSAGVTLLYMLIGKTSFPGEPEQSLKEIAKLRGSEELWEVAKLHDRETSFPVELFDDRYLQSYDIETWCKLHTKRPEFVDKIPKSLFDLIEKCLTVNPRNRISVEDVLRHEFFASCNEIMRKTRMLQRGLGLETAADSRAV*