AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041104

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041104 MtrunA17_Chr8g0335821 96.703 364 12 0 279 642 1 364 0.0 713
MsaG041104 MtrunA17_Chr2g0280971 64.066 423 150 2 221 642 20 441 0.0 542
MsaG041104 MtrunA17_Chr2g0280991 61.871 417 159 0 226 642 5 421 0.0 533
MsaG041104 MtrunA17_Chr2g0323381 60.049 408 161 1 235 642 7 412 7.71e-167 483
MsaG041104 MtrunA17_Chr4g0022871 56.867 415 173 2 230 642 2 412 1.99e-161 469
MsaG041104 MtrunA17_Chr4g0022861 55.890 399 170 2 246 642 2 396 3.93e-150 439
MsaG041104 MtrunA17_Chr4g0022801 55.416 397 145 3 275 642 10 403 2.33e-134 399
MsaG041104 MtrunA17_Chr8g0335811 72.816 206 30 1 4 183 7 212 1.78e-90 280
MsaG041104 MtrunA17_Chr1g0184961 41.504 359 173 5 194 523 2 352 4.15e-77 249
MsaG041104 MtrunA17_Chr2g0280961 90.000 130 12 1 470 598 102 231 3.63e-73 235
MsaG041104 MtrunA17_Chr2g0280961 80.220 91 18 0 279 369 1 91 1.92e-41 150
MsaG041104 MtrunA17_Chr4g0020331 29.803 406 254 9 244 640 32 415 8.95e-36 139
MsaG041104 MtrunA17_Chr3g0129361 66.304 92 29 1 108 197 127 218 3.34e-35 132
MsaG041104 MtrunA17_Chr8g0383351 83.582 67 10 1 108 173 142 208 1.29e-32 125
MsaG041104 MtrunA17_Chr3g0103741 28.784 403 258 7 246 640 42 423 8.61e-32 128
MsaG041104 MtrunA17_Chr4g0063911 27.316 421 254 14 235 638 11 396 1.49e-26 112
MsaG041104 MtrunA17_Chr5g0418781 64.000 75 27 0 99 173 98 172 5.59e-26 108
MsaG041104 MtrunA17_Chr5g0432161 57.143 84 34 1 92 173 386 469 3.64e-25 110
MsaG041104 MtrunA17_Chr5g0432161 71.795 39 11 0 125 163 244 282 3.60e-12 69.3
MsaG041104 MtrunA17_Chr7g0248721 43.609 133 58 4 55 173 59 188 1.28e-24 102
MsaG041104 MtrunA17_Chr7g0224801 65.574 61 21 0 113 173 81 141 5.31e-24 99.4
MsaG041104 MtrunA17_Chr2g0294231 69.231 65 20 0 109 173 187 251 6.14e-24 104
MsaG041104 MtrunA17_Chr1g0194481 60.000 75 30 0 99 173 132 206 2.61e-23 101
MsaG041104 MtrunA17_Chr3g0106491 63.235 68 25 0 106 173 346 413 1.29e-22 102
MsaG041104 MtrunA17_Chr3g0106491 58.491 53 18 1 115 163 184 236 1.28e-12 70.5
MsaG041104 MtrunA17_Chr4g0051881 68.254 63 20 0 111 173 164 226 2.07e-22 99.0
MsaG041104 MtrunA17_Chr4g0068541 64.615 65 23 0 109 173 192 256 6.27e-22 99.0
MsaG041104 MtrunA17_Chr3g0079691 61.765 68 26 0 106 173 476 543 6.30e-22 100
MsaG041104 MtrunA17_Chr3g0079691 71.429 42 12 0 125 166 280 321 1.25e-13 74.3
MsaG041104 MtrunA17_Chr8g0386241 35.507 138 79 3 70 198 40 176 7.65e-22 93.6
MsaG041104 MtrunA17_Chr7g0218081 59.420 69 28 0 105 173 390 458 9.98e-22 99.4
MsaG041104 MtrunA17_Chr7g0218081 71.429 42 12 0 125 166 228 269 1.18e-13 73.9
MsaG041104 MtrunA17_Chr3g0129321 36.242 149 85 2 25 173 28 166 1.51e-21 93.2
MsaG041104 MtrunA17_Chr1g0150801 39.568 139 72 4 40 173 19 150 5.46e-21 90.5
MsaG041104 MtrunA17_Chr5g0445311 57.143 70 30 0 104 173 343 412 6.29e-21 97.1
MsaG041104 MtrunA17_Chr5g0445311 53.226 62 29 0 102 163 177 238 6.78e-12 68.6
MsaG041104 MtrunA17_Chr7g0270191 57.333 75 32 0 99 173 119 193 8.71e-21 93.2
MsaG041104 MtrunA17_Chr3g0101131 58.571 70 29 0 104 173 394 463 9.82e-21 96.7
MsaG041104 MtrunA17_Chr3g0101131 71.795 39 11 0 125 163 243 281 1.62e-11 67.4
MsaG041104 MtrunA17_Chr5g0443131 63.492 63 23 0 111 173 143 205 2.46e-20 92.4
MsaG041104 MtrunA17_Chr5g0418191 58.824 68 28 0 106 173 301 368 4.50e-20 93.6
MsaG041104 MtrunA17_Chr5g0418191 76.923 39 9 0 125 163 151 189 1.59e-13 73.2
MsaG041104 MtrunA17_Chr7g0249311 57.353 68 29 0 106 173 373 440 6.38e-20 94.4
MsaG041104 MtrunA17_Chr7g0249311 74.359 39 10 0 125 163 179 217 1.63e-13 73.9
MsaG041104 MtrunA17_Chr7g0249301 57.971 69 29 0 105 173 272 340 9.74e-20 91.7
MsaG041104 MtrunA17_Chr7g0249301 65.854 41 14 0 123 163 60 100 1.26e-11 67.0
MsaG041104 MtrunA17_Chr8g0383251 45.556 90 43 2 84 173 73 156 1.66e-19 86.7
MsaG041104 MtrunA17_Chr1g0184971 61.905 63 24 0 526 588 2 64 1.71e-19 83.6
MsaG041104 MtrunA17_Chr4g0004911 55.882 68 30 0 106 173 317 384 3.86e-19 90.9
MsaG041104 MtrunA17_Chr4g0004911 57.447 47 20 0 125 171 184 230 9.36e-11 64.7
MsaG041104 MtrunA17_Chr4g0042831 55.882 68 30 0 106 173 259 326 4.17e-19 89.7
MsaG041104 MtrunA17_Chr4g0042831 69.231 39 12 0 125 163 145 183 9.84e-11 64.3
MsaG041104 MtrunA17_Chr7g0243891 52.778 72 34 0 102 173 351 422 7.77e-19 90.5
MsaG041104 MtrunA17_Chr7g0243891 69.231 39 12 0 125 163 216 254 1.20e-11 67.8
MsaG041104 MtrunA17_Chr1g0188941 50.685 73 36 0 102 174 28 100 1.08e-18 82.0
MsaG041104 MtrunA17_Chr7g0263531 53.030 66 31 0 108 173 344 409 3.02e-15 78.6
MsaG041104 MtrunA17_Chr7g0263531 73.684 38 10 0 126 163 190 227 9.15e-12 67.8
MsaG041104 MtrunA17_Chr7g0238921 64.583 48 17 0 125 172 124 171 8.67e-15 75.9
MsaG041104 MtrunA17_Chr3g0139021 34.884 129 75 5 50 172 237 362 9.78e-14 73.6
MsaG041104 MtrunA17_Chr1g0189391 40.206 97 41 3 92 172 50 145 9.89e-14 72.8
MsaG041104 MtrunA17_Chr4g0074001 46.269 67 36 0 106 172 340 406 1.33e-13 73.9
MsaG041104 MtrunA17_Chr5g0447701 43.038 79 45 0 110 188 237 315 3.43e-13 71.6
MsaG041104 MtrunA17_Chr3g0126391 35.455 110 55 5 66 167 77 178 2.14e-12 68.9
MsaG041104 MtrunA17_Chr7g0270831 36.792 106 54 3 79 173 162 265 2.71e-12 69.3
MsaG041104 MtrunA17_Chr6g0485431 34.058 138 66 5 60 172 65 202 3.44e-12 68.6
MsaG041104 MtrunA17_Chr1g0147881 42.105 76 40 2 98 172 238 310 5.34e-12 67.8
MsaG041104 MtrunA17_Chr4g0075201 39.506 81 46 2 94 173 238 316 5.68e-12 68.6
MsaG041104 MtrunA17_Chr2g0288871 28.571 126 79 3 59 173 229 354 9.24e-12 68.2
MsaG041104 MtrunA17_Chr4g0060271 43.939 66 36 1 109 173 239 304 1.05e-11 68.2
MsaG041104 MtrunA17_Chr2g0322391 43.284 67 37 1 108 173 332 398 1.35e-11 67.8
MsaG041104 MtrunA17_Chr7g0228941 45.000 60 31 2 114 172 42 100 1.56e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041104 AT4G05120.1 59.951 412 162 2 231 642 6 414 3.32e-175 504
MsaG041104 AT4G05120.2 59.951 412 162 2 231 642 6 414 3.32e-175 504
MsaG041104 AT4G05140.1 57.518 419 172 2 226 642 1 415 8.57e-167 483
MsaG041104 AT4G05110.3 57.524 412 172 2 231 642 6 414 4.46e-166 481
MsaG041104 AT4G05110.1 57.524 412 172 2 231 642 6 414 4.46e-166 481
MsaG041104 AT4G05110.2 57.282 412 173 2 231 642 6 414 8.23e-165 479
MsaG041104 AT4G05130.1 56.280 414 174 2 231 642 6 414 1.90e-162 472
MsaG041104 AT1G61630.1 50.120 415 206 1 228 642 1 414 4.31e-143 422
MsaG041104 AT3G09990.1 48.768 406 203 2 239 642 13 415 6.84e-130 389
MsaG041104 AT2G44745.1 55.838 197 60 8 13 184 17 211 1.91e-50 174
MsaG041104 AT1G70330.1 28.851 409 257 9 244 640 64 450 2.15e-36 142
MsaG041104 AT4G39410.1 78.082 73 16 0 101 173 199 271 2.80e-34 133
MsaG041104 AT1G02630.1 25.891 421 268 9 230 640 3 389 6.74e-32 128
MsaG041104 AT2G46130.1 65.152 66 23 0 106 171 11 76 6.65e-28 108
MsaG041104 AT1G69310.4 64.000 75 27 0 99 173 121 195 3.04e-27 112
MsaG041104 AT1G69310.2 64.000 75 27 0 99 173 121 195 3.04e-27 112
MsaG041104 AT1G69310.1 64.000 75 27 0 99 173 121 195 3.04e-27 112
MsaG041104 AT5G41570.1 56.410 78 34 0 96 173 69 146 3.96e-26 105
MsaG041104 AT1G64000.1 60.811 74 29 0 100 173 89 162 5.37e-26 106
MsaG041104 AT5G13080.1 51.020 98 48 0 93 190 35 132 2.32e-24 99.8
MsaG041104 AT4G18170.1 61.333 75 29 0 99 173 146 220 3.24e-23 101
MsaG041104 AT2G46130.2 59.091 66 23 1 106 171 11 72 1.36e-22 93.2
MsaG041104 AT4G26440.1 48.649 111 42 2 77 173 311 420 1.39e-22 102
MsaG041104 AT4G26440.1 71.429 42 12 0 125 166 178 219 6.47e-14 75.5
MsaG041104 AT3G01970.1 53.165 79 37 0 112 190 52 130 1.85e-22 94.4
MsaG041104 AT5G49520.1 67.692 65 21 0 109 173 205 269 1.99e-22 100
MsaG041104 AT1G69310.3 58.667 75 27 1 99 173 121 191 3.14e-22 97.8
MsaG041104 AT3G01080.2 58.333 72 30 0 102 173 321 392 4.53e-22 100
MsaG041104 AT3G01080.2 69.048 42 13 0 125 166 205 246 6.43e-13 72.0
MsaG041104 AT5G56270.1 63.235 68 25 0 106 173 468 535 6.88e-22 100
MsaG041104 AT5G56270.1 69.048 42 13 0 125 166 273 314 8.00e-13 72.0
MsaG041104 AT3G01080.1 58.333 72 30 0 102 173 283 354 8.91e-22 99.0
MsaG041104 AT3G01080.1 69.048 42 13 0 125 166 167 208 1.06e-12 70.9
MsaG041104 AT5G26170.1 62.903 62 23 0 112 173 100 161 1.44e-21 92.8
MsaG041104 AT5G07100.1 48.352 91 47 0 83 173 192 282 1.96e-21 95.9
MsaG041104 AT5G07100.1 68.421 38 12 0 125 162 117 154 9.56e-11 63.9
MsaG041104 AT1G29860.1 49.495 99 48 2 109 205 120 218 2.22e-21 95.1
MsaG041104 AT2G47260.1 57.333 75 32 0 99 173 148 222 3.09e-21 95.9
MsaG041104 AT5G07100.2 48.352 91 47 0 83 173 99 189 3.57e-21 92.8
MsaG041104 AT5G07100.4 48.352 91 47 0 83 173 99 189 3.57e-21 92.8
MsaG041104 AT5G07100.5 48.352 91 47 0 83 173 99 189 3.57e-21 92.8
MsaG041104 AT5G07100.3 48.352 91 47 0 83 173 99 189 3.57e-21 92.8
MsaG041104 AT2G38470.1 61.765 68 26 0 106 173 343 410 4.01e-21 97.8
MsaG041104 AT2G38470.1 69.231 39 12 0 125 163 184 222 5.36e-11 65.9
MsaG041104 AT4G26640.7 37.589 141 66 4 106 230 322 456 4.42e-21 97.8
MsaG041104 AT4G26640.7 70.732 41 12 0 123 163 169 209 8.94e-12 68.6
MsaG041104 AT4G26640.5 37.589 141 66 4 106 230 186 320 4.71e-21 96.3
MsaG041104 AT4G26640.5 70.732 41 12 0 123 163 33 73 1.41e-11 67.0
MsaG041104 AT4G26640.6 37.589 141 66 4 106 230 186 320 4.71e-21 96.3
MsaG041104 AT4G26640.6 70.732 41 12 0 123 163 33 73 1.41e-11 67.0
MsaG041104 AT4G26640.4 37.589 141 66 4 106 230 186 320 4.71e-21 96.3
MsaG041104 AT4G26640.4 70.732 41 12 0 123 163 33 73 1.41e-11 67.0
MsaG041104 AT4G26640.3 37.589 141 66 4 106 230 186 320 4.71e-21 96.3
MsaG041104 AT4G26640.3 70.732 41 12 0 123 163 33 73 1.41e-11 67.0
MsaG041104 AT4G26640.1 37.589 141 66 4 106 230 290 424 5.51e-21 97.1
MsaG041104 AT4G26640.1 70.732 41 12 0 123 163 137 177 1.08e-11 68.2
MsaG041104 AT4G26640.2 37.589 141 66 4 106 230 362 496 6.51e-21 97.4
MsaG041104 AT4G26640.2 70.732 41 12 0 123 163 209 249 9.72e-12 68.6
MsaG041104 AT5G46350.1 63.492 63 23 0 111 173 169 231 2.73e-20 92.8
MsaG041104 AT1G13960.1 53.247 77 36 0 97 173 381 457 3.02e-20 95.1
MsaG041104 AT1G13960.1 71.795 39 11 0 125 163 229 267 1.28e-11 67.8
MsaG041104 AT1G13960.2 52.564 78 37 0 97 174 228 305 9.11e-20 92.0
MsaG041104 AT1G13960.2 71.795 39 11 0 125 163 76 114 3.18e-11 65.9
MsaG041104 AT5G64810.1 43.137 102 55 2 75 173 57 158 1.26e-19 87.8
MsaG041104 AT2G03340.1 55.556 72 32 0 102 173 392 463 1.26e-19 93.2
MsaG041104 AT2G03340.1 74.359 39 10 0 125 163 250 288 3.64e-12 69.7
MsaG041104 AT2G30250.1 53.086 81 38 0 93 173 296 376 1.93e-19 91.3
MsaG041104 AT3G62340.1 56.757 74 30 1 100 173 95 166 1.26e-17 84.0
MsaG041104 AT1G55600.1 50.000 68 34 0 106 173 288 355 2.51e-16 82.8
MsaG041104 AT2G04880.1 41.237 97 50 1 84 173 259 355 1.43e-15 80.1
MsaG041104 AT2G04880.1 65.854 41 14 0 123 163 109 149 2.36e-11 67.0
MsaG041104 AT2G04880.2 41.237 97 50 1 84 173 235 331 1.88e-15 79.7
MsaG041104 AT2G04880.2 65.854 41 14 0 123 163 109 149 2.32e-11 67.0
MsaG041104 AT1G02630.2 26.630 184 131 3 230 409 3 186 1.91e-15 78.6
MsaG041104 AT2G37260.2 58.182 55 23 0 120 174 262 316 6.05e-15 77.0
MsaG041104 AT2G37260.2 70.732 41 12 0 126 166 84 124 3.37e-11 65.9
MsaG041104 AT2G37260.3 58.182 55 23 0 120 174 264 318 6.17e-15 77.0
MsaG041104 AT2G37260.3 70.732 41 12 0 126 166 86 126 3.41e-11 65.9
MsaG041104 AT2G37260.1 59.259 54 22 0 120 173 344 397 7.62e-15 77.8
MsaG041104 AT2G37260.1 70.732 41 12 0 126 166 166 206 3.09e-11 66.2
MsaG041104 AT3G01080.3 69.048 42 13 0 125 166 205 246 1.15e-13 73.2
MsaG041104 AT5G15130.2 39.506 81 48 1 94 173 154 234 1.25e-13 74.3
MsaG041104 AT5G15130.1 39.506 81 48 1 94 173 196 276 1.68e-13 73.9
MsaG041104 AT2G21900.1 43.056 72 36 2 97 163 76 147 3.55e-13 69.3
MsaG041104 AT5G43290.1 54.717 53 23 1 112 163 100 152 1.40e-12 68.9
MsaG041104 AT4G30935.1 49.180 61 31 0 112 172 318 378 1.83e-12 70.5
MsaG041104 AT2G23320.1 40.426 94 47 3 80 172 203 288 6.16e-12 67.8
MsaG041104 AT4G04450.2 39.535 86 45 2 95 173 162 247 1.05e-11 67.8
MsaG041104 AT4G12020.4 33.333 120 60 2 40 145 548 661 1.69e-11 68.2
MsaG041104 AT4G12020.10 33.333 120 60 2 40 145 548 661 1.75e-11 68.2
MsaG041104 AT4G12020.2 33.333 120 60 2 40 145 548 661 1.78e-11 68.2
MsaG041104 AT4G12020.5 33.333 120 60 2 40 145 481 594 1.78e-11 68.2
MsaG041104 AT4G12020.1 33.333 120 60 2 40 145 548 661 1.89e-11 68.2
MsaG041104 AT4G12020.9 33.333 120 60 2 40 145 548 661 1.89e-11 68.2
MsaG041104 AT4G12020.3 33.333 120 60 2 40 145 548 661 1.90e-11 68.2
MsaG041104 AT4G12020.7 33.333 120 60 2 40 145 548 661 1.92e-11 68.2
MsaG041104 AT4G12020.6 33.333 120 60 2 40 145 548 661 2.07e-11 68.2
MsaG041104 AT4G12020.8 33.333 120 60 2 40 145 548 661 2.07e-11 68.2
MsaG041104 AT4G24240.1 40.260 77 42 2 97 172 256 329 2.75e-11 66.2
MsaG041104 AT2G30590.1 43.210 81 42 2 93 172 284 361 3.04e-11 66.2
MsaG041104 AT4G12020.11 33.333 120 60 2 40 145 548 661 3.05e-11 67.4
MsaG041104 AT4G31550.2 42.105 76 40 2 98 172 221 293 3.64e-11 65.5
MsaG041104 AT4G04450.1 39.535 86 45 2 95 173 256 341 4.70e-11 66.2
MsaG041104 AT5G28650.1 35.789 95 55 3 79 172 221 310 5.27e-11 65.1
MsaG041104 AT1G62300.1 43.284 67 37 1 108 173 295 361 9.33e-11 65.1

Find 132 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAATGAAACTTGGATAAT+TGG 0.134491 8:-1796107 None:intergenic
GAGCTGCTCTTAGTTATTTA+TGG 0.141291 8:+1841481 MsaT041104.1:CDS
CTTAGTTATTTATGGCAAAT+TGG 0.226682 8:+1841489 MsaT041104.1:CDS
CGGTTAAAGGCAACCTTAAA+AGG 0.236786 8:+1828393 MsaT041104.1:CDS
AAGTTAATATGTTTCATTCT+AGG 0.241448 8:+1825084 MsaT041104.1:CDS
ACAAGTTTGCGGAACTTATA+TGG 0.243472 8:+1826952 MsaT041104.1:CDS
TATGTGTCCTGAGTTCATTC+AGG 0.274841 8:+1827335 MsaT041104.1:CDS
CAACACATATACCACTTAAA+AGG 0.278810 8:-1841458 None:intergenic
ACTTATAACAACATTGATAT+TGG 0.281501 8:+1826906 MsaT041104.1:CDS
AAGTTAATATGTTTCATTCT+TGG 0.290059 8:+1818974 MsaT041104.1:CDS
ATTGAACAAAACCCATTTCA+TGG 0.296272 8:-1796160 None:intergenic
ATTGGTGGTATAACTGCTTC+TGG 0.300007 8:+1827864 MsaT041104.1:CDS
TGTAAAATACTACCGGTTAA+AGG 0.304342 8:+1828380 MsaT041104.1:CDS
ACTAAACAACTTGTGGACTA+TGG 0.310523 8:+1819015 MsaT041104.1:CDS
ACTAAACAACTTGTGGACTA+TGG 0.310523 8:+1825125 MsaT041104.1:CDS
TTCTCATCACTCACAGCTTT+GGG 0.313305 8:-1796822 None:intergenic
CTCTCAAGCTATCCATGAAA+TGG 0.321044 8:+1796148 MsaT041104.1:CDS
ATTCTCATCACTCACAGCTT+TGG 0.324655 8:-1796823 None:intergenic
CTTGTTCAAGGTGGCGTCTC+TGG 0.324734 8:+1827300 MsaT041104.1:CDS
GATAACAGTGTACAACGTTA+TGG 0.337628 8:+1841053 MsaT041104.1:CDS
ATTGAGAAAGTTGAGAAGGT+TGG 0.338269 8:-1796229 None:intergenic
GATGTTATGCAGGCCTTTAT+TGG 0.346370 8:+1827846 MsaT041104.1:intron
GGAATGAAACTTGGATAATT+GGG 0.346794 8:-1796106 None:intergenic
TTGAGAAAGTTGAGAAGGTT+GGG 0.349918 8:-1796228 None:intergenic
CCTTTCTAGATTCCAACTTC+AGG 0.353465 8:-1841110 None:intergenic
AGCAGTTATACCACCAATAA+AGG 0.364549 8:-1827859 None:intergenic
ACACTAACCTTGTAACCTTT+AGG 0.368406 8:-1841282 None:intergenic
GATCCTACTTGTATCTTACT+TGG 0.381344 8:+1841215 MsaT041104.1:CDS
GGAATCTAGAAAGGGATTAC+TGG 0.392373 8:+1841119 MsaT041104.1:CDS
CATCTACATCACTTCTTGTC+TGG 0.400937 8:-1797205 None:intergenic
GGTTACAAATGGAGGAAGTA+TGG 0.401960 8:+1797239 MsaT041104.1:CDS
ATATGTTTCATTCTTGGAGC+TGG 0.408091 8:+1818980 MsaT041104.1:CDS
GGATTCTTGTATGAAGATAC+TGG 0.409827 8:+1829218 MsaT041104.1:CDS
CTTTCTCAATCTCTAAATTC+CGG 0.416525 8:+1796242 MsaT041104.1:CDS
CCTCATCAAATTCCTGAAGT+TGG 0.417371 8:+1841098 MsaT041104.1:CDS
ATATGTTTCATTCTAGGAGC+TGG 0.439703 8:+1825090 MsaT041104.1:CDS
CCTGAAGTTGGAATCTAGAA+AGG 0.455008 8:+1841110 MsaT041104.1:CDS
CAAAATATGGTGACCAAGGT+TGG 0.456708 8:+1841190 MsaT041104.1:CDS
ATCTAGCAACATCAGGGAAA+GGG 0.457104 8:+1827217 MsaT041104.1:CDS
ATTATAATTACCTTGGATGA+AGG 0.467552 8:-1797283 None:intergenic
TAGTTGGATCTAGCAACATC+AGG 0.472204 8:+1827210 MsaT041104.1:intron
TCTCAAGCTATCCATGAAAT+GGG 0.477777 8:+1796149 MsaT041104.1:CDS
AGAGATTGAGAAAGTTGAGA+AGG 0.481462 8:-1796233 None:intergenic
ATTGCACATGCTCTTGTTCA+AGG 0.483326 8:+1827288 MsaT041104.1:CDS
GGCGTTGGTGGTGCTACGGC+CGG 0.484990 8:-1796261 None:intergenic
AGAATCCAGGCATTATAGAC+AGG 0.485034 8:-1829202 None:intergenic
CTAAACAACTTGTGGACTAT+GGG 0.492806 8:+1819016 MsaT041104.1:CDS
CTAAACAACTTGTGGACTAT+GGG 0.492806 8:+1825126 MsaT041104.1:CDS
ATAACCCTGTCTATAATGCC+TGG 0.495529 8:+1829197 MsaT041104.1:CDS
ATGGGAACGGACTTCGCAAA+GGG 0.496845 8:+1827937 MsaT041104.1:CDS
TGTGACGGCTGCACCGGTAG+TGG 0.499548 8:-1796282 None:intergenic
CAACTTCAGGAATTTGATGA+GGG 0.501106 8:-1841097 None:intergenic
TTTGCCGTGAAGTAAAATGC+TGG 0.501579 8:-1841171 None:intergenic
AATGTTGTTATAAGTGCAAA+TGG 0.507009 8:-1826898 None:intergenic
GGTAAACTTGGAATGAAACT+TGG 0.516805 8:-1796115 None:intergenic
AGGTATTTGAGAGGTAAACT+TGG 0.517455 8:-1796127 None:intergenic
CAAGTTGTTTAGTGCAATGA+GGG 0.519117 8:-1819005 None:intergenic
CAAGTTGTTTAGTGCAATGA+GGG 0.519117 8:-1825115 None:intergenic
AGCACCACCAACGCCACTAC+CGG 0.520442 8:+1796269 MsaT041104.1:CDS
GCTGCACACATAACAATTGT+CGG 0.522647 8:+1800962 MsaT041104.1:CDS
CATTTCATGGATAGCTTGAG+AGG 0.523950 8:-1796147 None:intergenic
CTGAAGTTGGAATCTAGAAA+GGG 0.524121 8:+1841111 MsaT041104.1:CDS
ATCCTACTTGTATCTTACTT+GGG 0.530008 8:+1841216 MsaT041104.1:CDS
TACACTGTTATCAAAACAAG+TGG 0.532700 8:-1841042 None:intergenic
TGCTTGATGATGGTTACAAA+TGG 0.537448 8:+1797228 MsaT041104.1:CDS
TGAAACATATTAACTTCGCT+CGG 0.538192 8:-1818967 None:intergenic
TGAAACATATTAACTTCGCT+CGG 0.538192 8:-1825077 None:intergenic
AGAAGAGCAAGGCGAAAGTG+AGG 0.541509 8:+1797153 MsaT041104.1:CDS
TCTAGCAACATCAGGGAAAG+GGG 0.542365 8:+1827218 MsaT041104.1:CDS
TTGCCGTGAAGTAAAATGCT+GGG 0.542732 8:-1841170 None:intergenic
TAGTGGCGTTGGTGGTGCTA+CGG 0.543122 8:-1796265 None:intergenic
TACTTTGCAGTGTTAAGCTT+AGG 0.544291 8:+1828291 MsaT041104.1:intron
CTGCACACATAACAATTGTC+GGG 0.546740 8:+1800963 MsaT041104.1:CDS
TGCACTGGAAATACTAGTGA+TGG 0.547346 8:+1796846 MsaT041104.1:CDS
TGTCCATTGTAAAATACTAC+CGG 0.550599 8:+1828373 MsaT041104.1:CDS
ATATATAGGTGGAGGAGTGG+AGG 0.551301 8:+1797125 MsaT041104.1:intron
GGCTGCACCGGTAGTGGCGT+TGG 0.551478 8:-1796276 None:intergenic
GTTATGCAGGCCTTTATTGG+TGG 0.553912 8:+1827849 MsaT041104.1:intron
TCATTGCACTAAACAACTTG+TGG 0.554760 8:+1819008 MsaT041104.1:CDS
TCATTGCACTAAACAACTTG+TGG 0.554760 8:+1825118 MsaT041104.1:CDS
GTATCTTCATACAAGAATCC+AGG 0.557432 8:-1829215 None:intergenic
ATGATATATAGGTGGAGGAG+TGG 0.558137 8:+1797122 MsaT041104.1:intron
TGCACCGGTAGTGGCGTTGG+TGG 0.563104 8:-1796273 None:intergenic
GCTTCTGGAGTTGTAGCTTG+TGG 0.563160 8:+1827879 MsaT041104.1:CDS
CCAACTTCAGGAATTTGATG+AGG 0.563722 8:-1841098 None:intergenic
TAGAGTTGTGACGGCTGCAC+CGG 0.565883 8:-1796288 None:intergenic
GATACTGGACAACACAAGCT+AGG 0.569182 8:+1829233 MsaT041104.1:CDS
GAAGGTTGGGATTGTGTAGA+GGG 0.569637 8:-1796215 None:intergenic
TCTTACTTGGGACTAACCAA+TGG 0.570300 8:+1841228 MsaT041104.1:CDS
CTTGTCTGGAAACAAAACCT+TGG 0.574196 8:-1797191 None:intergenic
GATGTAGATGTGCTTGATGA+TGG 0.574505 8:+1797218 MsaT041104.1:CDS
GATCTAGCAACATCAGGGAA+AGG 0.574518 8:+1827216 MsaT041104.1:CDS
GTGAGTGATGAGAATTGCAC+TGG 0.574611 8:+1796831 MsaT041104.1:CDS
CAAGGATCAATACAAGTTTG+CGG 0.575541 8:+1826941 MsaT041104.1:CDS
TTGTGAATGCTCGCATAGGA+TGG 0.576763 8:-1826864 None:intergenic
AGAAGGTTGGGATTGTGTAG+AGG 0.578720 8:-1796216 None:intergenic
TTGTTATAAGTGCAAATGGC+TGG 0.580480 8:-1826894 None:intergenic
GACTATTTCTAATGAAAGTG+TGG 0.585322 8:+1824946 MsaT041104.1:CDS
CAAGTAGGATCATCCAACCT+TGG 0.585770 8:-1841203 None:intergenic
GGAGGAAACTTAGAGAGCCA+AGG 0.588730 8:+1797174 MsaT041104.1:CDS
ACAAACAGTGAGATAACCAT+TGG 0.589435 8:-1841244 None:intergenic
TGGAGGAGCAGAGAAGAGCA+AGG 0.596711 8:+1797142 MsaT041104.1:CDS
CAGATTGTGAATGCTCGCAT+AGG 0.596823 8:-1826868 None:intergenic
ACAAGTTGTTTAGTGCAATG+AGG 0.597851 8:-1819006 None:intergenic
ACAAGTTGTTTAGTGCAATG+AGG 0.597851 8:-1825116 None:intergenic
AGTGGTACCTAAAGGTTACA+AGG 0.602697 8:+1841275 MsaT041104.1:CDS
CTAGTGATGGCAACAACACA+TGG 0.604178 8:+1796859 MsaT041104.1:CDS
TATTGGCTCACTACGAGTCA+AGG 0.609403 8:+1826923 MsaT041104.1:CDS
TCAAGAATAGCCTTCATCCA+AGG 0.610641 8:+1797273 MsaT041104.1:CDS
AACTTCAGGAATTTGATGAG+GGG 0.615037 8:-1841096 None:intergenic
GTATACACAGTGGTACCTAA+AGG 0.615782 8:+1841267 MsaT041104.1:CDS
TATGGGAACGGACTTCGCAA+AGG 0.619136 8:+1827936 MsaT041104.1:CDS
AGAGCAAGGCGAAAGTGAGG+AGG 0.623548 8:+1797156 MsaT041104.1:CDS
AGATTCTTACAGGAAACACT+TGG 0.624044 8:-1825161 None:intergenic
CTAGCAACATCAGGGAAAGG+GGG 0.626556 8:+1827219 MsaT041104.1:CDS
AGACCAACCTGAATGAACTC+AGG 0.627844 8:-1827342 None:intergenic
GACTATGTCTAATGAAAGTG+TGG 0.627977 8:+1818354 MsaT041104.1:CDS
AGTTGGATCTAGCAACATCA+GGG 0.634333 8:+1827211 MsaT041104.1:intron
AGATTCTTACATGAAACACT+TGG 0.635480 8:-1819051 None:intergenic
GTCCCAAGTAAGATACAAGT+AGG 0.635585 8:-1841218 None:intergenic
TAGCTTGAGAGGTATTTGAG+AGG 0.639081 8:-1796136 None:intergenic
ACAAGAATGCTTAAGCCACA+AGG 0.644550 8:+1829121 MsaT041104.1:CDS
ACTCTCAGAAGATTGTCGTA+TGG 0.646484 8:+1801005 MsaT041104.1:CDS
TCTGAAGAAATAGTTCCTTG+TGG 0.646789 8:-1829136 None:intergenic
GACAACACAAGCTAGGAACA+TGG 0.660524 8:+1829240 MsaT041104.1:CDS
TTGATGATGGTTACAAATGG+AGG 0.665075 8:+1797231 MsaT041104.1:CDS
ACGGCAAAATATGGTGACCA+AGG 0.672389 8:+1841186 MsaT041104.1:CDS
GAATCCAGGCATTATAGACA+GGG 0.672836 8:-1829201 None:intergenic
GCACATGCTCTTGTTCAAGG+TGG 0.676830 8:+1827291 MsaT041104.1:CDS
TCAAAATGTTGAGACAGACC+AGG 0.700472 8:+1828455 MsaT041104.1:CDS
TGGGAACGGACTTCGCAAAG+GGG 0.718165 8:+1827938 MsaT041104.1:CDS
CACTGTTTGTGTATACACAG+TGG 0.732442 8:+1841257 MsaT041104.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 1796101 1841518 1796101 ID=MsaG041104
Chr8 mRNA 1796101 1841518 1796101 ID=MsaT041104.1;Parent=MsaG041104
Chr8 exon 1796101 1796298 1796101 ID=MsaT041104.1.exon1;Parent=MsaT041104.1
Chr8 CDS 1796101 1796298 1796101 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1796807 1796880 1796807 ID=MsaT041104.1.exon2;Parent=MsaT041104.1
Chr8 CDS 1796807 1796880 1796807 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1797133 1797294 1797133 ID=MsaT041104.1.exon3;Parent=MsaT041104.1
Chr8 CDS 1797133 1797294 1797133 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1800951 1801026 1800951 ID=MsaT041104.1.exon4;Parent=MsaT041104.1
Chr8 CDS 1800951 1801026 1800951 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1818343 1818393 1818343 ID=MsaT041104.1.exon5;Parent=MsaT041104.1
Chr8 CDS 1818343 1818393 1818343 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1818959 1819063 1818959 ID=MsaT041104.1.exon6;Parent=MsaT041104.1
Chr8 CDS 1818959 1819063 1818959 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1824935 1824985 1824935 ID=MsaT041104.1.exon7;Parent=MsaT041104.1
Chr8 CDS 1824935 1824985 1824935 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1825069 1825173 1825069 ID=MsaT041104.1.exon8;Parent=MsaT041104.1
Chr8 CDS 1825069 1825173 1825069 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1826859 1827014 1826859 ID=MsaT041104.1.exon9;Parent=MsaT041104.1
Chr8 CDS 1826859 1827014 1826859 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1827213 1827356 1827213 ID=MsaT041104.1.exon10;Parent=MsaT041104.1
Chr8 CDS 1827213 1827356 1827213 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1827858 1827963 1827858 ID=MsaT041104.1.exon11;Parent=MsaT041104.1
Chr8 CDS 1827858 1827963 1827858 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1828301 1828479 1828301 ID=MsaT041104.1.exon12;Parent=MsaT041104.1
Chr8 CDS 1828301 1828479 1828301 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1829101 1829261 1829101 ID=MsaT041104.1.exon13;Parent=MsaT041104.1
Chr8 CDS 1829101 1829261 1829101 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1841038 1841296 1841038 ID=MsaT041104.1.exon14;Parent=MsaT041104.1
Chr8 CDS 1841038 1841296 1841038 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Chr8 exon 1841417 1841518 1841417 ID=MsaT041104.1.exon15;Parent=MsaT041104.1
Chr8 CDS 1841417 1841518 1841417 ID=cds.MsaT041104.1;Parent=MsaT041104.1
Gene Sequence

>MsaT041104.1

ATGTTCCCAATTATCCAAGTTTCATTCCAAGTTTACCTCTCAAATACCTCTCAAGCTATCCATGAAATGGGTTTTGTTCAATTTGAAGAAAATCAAGTTCTTAGCTTCTTGTCACCCTCTACACAATCCCAACCTTCTCAACTTTCTCAATCTCTAAATTCCGGCCGTAGCACCACCAACGCCACTACCGGTGCAGCCGTGAGAACTCTAGATCCCAAAGCTGTGAGTGATGAGAATTGCACTGGAAATACTAGTGATGGCAACAACACATGGTGGAGGAGTGGAGGAGCAGAGAAGAGCAAGGCGAAAGTGAGGAGGAAACTTAGAGAGCCAAGGTTTTGTTTCCAGACAAGAAGTGATGTAGATGTGCTTGATGATGGTTACAAATGGAGGAAGTATGGTCAGAAAGTTGTCAAGAATAGCCTTCATCCAAGAAGTTATTATCGCTGCACACATAACAATTGTCGGGTAAAAAAAAGAGTTGAACGACTCTCAGAAGATTGTCGTATGGTCATTAGTATGACTATGTCTAATGAAAGTGTGGCACCAGAAAAAATGGAGGGGAAATTCCGAGCGAAGTTAATATGTTTCATTCTTGGAGCTGGCTCCCTCATTGCACTAAACAACTTGTGGACTATGGGAGATTACTACTACCAAGTGTTTCATGTCATTAGCATGACTATTTCTAATGAAAGTGTGGCACCAGAAAAAATGGAGGGGAAATTCCGAGCGAAGTTAATATGTTTCATTCTAGGAGCTGGCTCCCTCATTGCACTAAACAACTTGTGGACTATGGGAGATTACTACTACCAAGTGTTTCCTAAATACCATCCTATGCGAGCATTCACAATCTGTTACCAGCCATTTGCACTTATAACAACATTGATATTGGCTCACTACGAGTCAAGGATCAATACAAGTTTGCGGAACTTATATGGATATGCACTTTTTTTTGTTCTCTCTTTCTTGGTCATTGTTTTGGATCTAGCAACATCAGGGAAAGGGGGAATTGGAACTTTCATCGGATTATGCGCTTTTTTTGCTTGTTTTGGAATTGCACATGCTCTTGTTCAAGGTGGCGTCTCTGGAGAACTATCTTCTATGTGTCCTGAGTTCATTCAGGCCTTTATTGGTGGTATAACTGCTTCTGGAGTTGTAGCTTGTGGTTTGAGACTTTTAACAAAATCTTATTTTGAGAAATATGGGAACGGACTTCGCAAAGGGGCTTTGTTAAGCTTAGGCATCTCTACACTCTTTGAGCTATTGAGCATTATCATGTATGCAATTTACTTTCCAAAGTTGTCCATTGTAAAATACTACCGGTTAAAGGCAACCTTAAAAGGAGCAAAAACAATTACAGATGGTCTTATTGCAACCGACATTCAAAATGTTGAGACAGACCAGGAAGTTGGAGTTGTTGCTAATCAACAAGAATGCTTAAGCCACAAGGAACTATTTCTTCAGAACATTGATTATGTTTTTGACGTAGTTATGATATATGTAATAACCCTGTCTATAATGCCTGGATTCTTGTATGAAGATACTGGACAACACAAGCTAGGAACATGGTATCCACTTGTTTTGATAACAGTGTACAACGTTATGGATCTAATAGCATCATATATTCCCCTCATCAAATTCCTGAAGTTGGAATCTAGAAAGGGATTACTGGTTGCTACTCTTTCTAGATTCTTGTTGATCCCAGCATTTTACTTCACGGCAAAATATGGTGACCAAGGTTGGATGATCCTACTTGTATCTTACTTGGGACTAACCAATGGTTATCTCACTGTTTGTGTATACACAGTGGTACCTAAAGGTTACAAGGGTCCGGAAAAAAATGCATTGGGCAATTTGCTTGCTTTGAGCCTTTTAAGTGGTATATGTGTTGGAGCTGCTCTTAGTTATTTATGGCAAATTGGAAAGTAA
Protein sequence

>MsaT041104.1

MFPIIQVSFQVYLSNTSQAIHEMGFVQFEENQVLSFLSPSTQSQPSQLSQSLNSGRSTTNATTGAAVRTLDPKAVSDENCTGNTSDGNNTWWRSGGAEKSKAKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHNNCRVKKRVERLSEDCRMVISMTMSNESVAPEKMEGKFRAKLICFILGAGSLIALNNLWTMGDYYYQVFHVISMTISNESVAPEKMEGKFRAKLICFILGAGSLIALNNLWTMGDYYYQVFPKYHPMRAFTICYQPFALITTLILAHYESRINTSLRNLYGYALFFVLSFLVIVLDLATSGKGGIGTFIGLCAFFACFGIAHALVQGGVSGELSSMCPEFIQAFIGGITASGVVACGLRLLTKSYFEKYGNGLRKGALLSLGISTLFELLSIIMYAIYFPKLSIVKYYRLKATLKGAKTITDGLIATDIQNVETDQEVGVVANQQECLSHKELFLQNIDYVFDVVMIYVITLSIMPGFLYEDTGQHKLGTWYPLVLITVYNVMDLIASYIPLIKFLKLESRKGLLVATLSRFLLIPAFYFTAKYGDQGWMILLVSYLGLTNGYLTVCVYTVVPKGYKGPEKNALGNLLALSLLSGICVGAALSYLWQIGK*