AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041157


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041157 MtrunA17_Chr8g0336681 87.164 335 37 5 1 332 14 345 0.0 545
MsaG041157 MtrunA17_Chr2g0333621 50.407 246 113 4 81 325 77 314 2.87e-66 211
MsaG041157 MtrunA17_Chr2g0333641 47.368 247 118 4 76 321 71 306 7.09e-63 202
MsaG041157 MtrunA17_Chr7g0249811 43.478 276 121 4 54 327 91 333 7.93e-62 200
MsaG041157 MtrunA17_Chr4g0002801 46.127 284 124 9 55 323 62 331 2.28e-59 194
MsaG041157 MtrunA17_Chr4g0002781 45.082 244 126 3 81 323 114 350 3.99e-56 186
MsaG041157 MtrunA17_Chr5g0400661 40.079 252 135 4 85 322 85 334 3.25e-50 170
MsaG041157 MtrunA17_Chr2g0280161 45.652 184 88 2 137 318 173 346 1.68e-47 163
MsaG041157 MtrunA17_Chr4g0054561 44.737 190 90 4 134 321 148 324 1.57e-46 160
MsaG041157 MtrunA17_Chr4g0049331 42.060 233 104 5 94 318 94 303 5.20e-46 158
MsaG041157 MtrunA17_Chr2g0333611 45.500 200 85 5 130 322 33 215 9.59e-46 155
MsaG041157 MtrunA17_Chr4g0054551 45.652 184 85 4 134 315 148 318 1.23e-45 158
MsaG041157 MtrunA17_Chr5g0400621 44.670 197 98 3 123 318 139 325 2.94e-45 157
MsaG041157 MtrunA17_Chr4g0054581 35.277 343 182 9 10 322 42 374 7.72e-45 157
MsaG041157 MtrunA17_Chr6g0468491 36.741 313 161 9 14 323 32 310 1.59e-43 152
MsaG041157 MtrunA17_Chr2g0333541 46.842 190 84 3 134 318 87 264 2.50e-41 145
MsaG041157 MtrunA17_Chr5g0400671 34.146 287 158 7 44 322 60 323 9.18e-40 142
MsaG041157 MtrunA17_Chr2g0307131 39.912 228 120 5 96 322 140 351 7.75e-39 140
MsaG041157 MtrunA17_Chr4g0033291 35.606 264 142 7 72 322 76 324 1.42e-37 137
MsaG041157 MtrunA17_Chr2g0307141 41.304 184 97 3 140 322 182 355 2.33e-37 137
MsaG041157 MtrunA17_Chr4g0033391 36.134 238 131 5 96 332 109 326 6.00e-37 135
MsaG041157 MtrunA17_Chr7g0225901 42.775 173 86 4 133 303 80 241 1.45e-35 129
MsaG041157 MtrunA17_Chr4g0054571 39.453 256 134 9 77 323 87 330 1.78e-33 126
MsaG041157 MtrunA17_Chr4g0033281 33.865 251 139 5 77 322 93 321 5.53e-30 116
MsaG041157 MtrunA17_Chr6g0468461 34.632 231 119 4 75 302 110 311 1.00e-29 116
MsaG041157 MtrunA17_Chr5g0400641 39.877 163 89 2 144 306 1 154 4.38e-29 109
MsaG041157 MtrunA17_Chr4g0054621 30.488 246 140 6 77 318 115 333 5.19e-29 114
MsaG041157 MtrunA17_Chr8g0336671 70.886 79 19 3 107 184 1 76 3.54e-28 104
MsaG041157 MtrunA17_Chr7g0233441 38.710 155 76 2 164 318 57 192 7.32e-27 105
MsaG041157 MtrunA17_Chr4g0002851 36.774 155 71 2 164 318 65 192 1.32e-25 101
MsaG041157 MtrunA17_Chr4g0002831 40.120 167 79 5 153 318 54 200 9.84e-25 99.4
MsaG041157 MtrunA17_Chr8g0366751 28.655 171 120 1 118 286 439 609 1.19e-13 71.6
MsaG041157 MtrunA17_Chr1g0185621 25.828 151 91 3 141 281 475 614 1.85e-11 65.1
MsaG041157 MtrunA17_Chr7g0252041 27.389 157 111 2 143 296 288 444 2.36e-11 64.7
MsaG041157 MtrunA17_Chr8g0346161 23.602 161 115 2 126 286 276 428 3.27e-11 63.9
MsaG041157 MtrunA17_Chr5g0411341 26.316 171 124 1 118 286 470 640 3.43e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041157 AT4G37850.2 46.701 197 93 4 127 321 138 324 4.25e-50 170
MsaG041157 AT4G37850.1 46.701 197 93 4 127 321 138 324 4.25e-50 170
MsaG041157 AT2G22770.1 43.147 197 106 2 133 323 124 320 4.40e-43 151
MsaG041157 AT2G22750.2 43.154 241 124 6 82 317 65 297 5.75e-40 142
MsaG041157 AT2G22750.5 43.154 241 124 6 82 317 89 321 7.07e-40 143
MsaG041157 AT2G22750.3 43.154 241 124 6 82 317 93 325 8.49e-40 143
MsaG041157 AT2G22760.1 43.956 182 91 4 140 318 118 291 2.58e-39 140
MsaG041157 AT2G22750.1 43.154 241 123 7 82 317 65 296 1.38e-38 139
MsaG041157 AT2G22750.4 43.154 241 123 7 82 317 93 324 2.25e-38 139
MsaG041157 AT2G22760.2 42.424 165 85 3 140 302 118 274 1.06e-33 125
MsaG041157 AT1G63650.3 27.586 174 112 5 117 279 382 552 3.26e-12 67.8
MsaG041157 AT1G63650.1 27.586 174 112 5 117 279 382 552 3.26e-12 67.8
MsaG041157 AT1G63650.2 27.586 174 112 5 117 279 382 552 3.26e-12 67.8
MsaG041157 AT4G29930.2 25.714 175 112 2 142 316 55 211 7.03e-12 64.7
MsaG041157 AT1G32640.1 28.649 185 108 4 113 287 422 592 1.25e-11 65.9
MsaG041157 AT4G09820.1 29.452 146 70 4 142 286 364 477 1.66e-11 65.5
MsaG041157 AT2G46510.1 28.302 159 87 3 123 281 377 508 1.69e-11 65.5
MsaG041157 AT3G26744.1 27.564 156 112 1 142 296 308 463 2.02e-11 65.1
MsaG041157 AT3G26744.4 27.564 156 112 1 142 296 308 463 2.02e-11 65.1
MsaG041157 AT3G26744.2 27.564 156 112 1 142 296 308 463 2.02e-11 65.1
MsaG041157 AT3G26744.6 27.564 156 112 1 142 296 308 463 2.15e-11 65.1
MsaG041157 AT4G17880.1 25.123 203 141 3 84 286 361 552 3.06e-11 64.7
MsaG041157 AT2G16910.1 26.768 198 127 5 143 327 316 508 4.13e-11 64.3
MsaG041157 AT4G29930.1 25.134 187 127 3 142 316 55 240 4.32e-11 62.8
MsaG041157 AT4G29930.6 25.843 178 111 3 142 316 55 214 4.50e-11 62.4
MsaG041157 AT5G46760.1 25.714 175 116 4 117 286 390 555 5.04e-11 64.3
MsaG041157 AT5G41315.1 25.000 184 112 6 117 279 415 593 8.66e-11 63.5
MsaG041157 AT5G41315.3 25.000 184 112 6 117 279 420 598 9.17e-11 63.5
MsaG041157 AT5G41315.2 25.000 184 112 6 117 279 423 601 9.97e-11 63.2

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAACATGTGACACTATT+AGG 0.173209 8:+2528297 None:intergenic
AGAGTTTCTTCTTAGATTCT+TGG 0.212913 8:+2528203 None:intergenic
AGTAGCAGTGTCTTGCCTTT+TGG 0.235787 8:-2525655 MsaT041157.1:CDS
GGATATTGTGGTCAGATTAA+TGG 0.259915 8:-2525710 MsaT041157.1:CDS
TGTCACATGTTCTTCTATTA+TGG 0.264783 8:-2528289 MsaT041157.1:CDS
ACATTATGGCTGAGAGAAAA+AGG 0.316242 8:-2528164 MsaT041157.1:CDS
ATCAAAAGAATATTTCTTAG+AGG 0.332771 8:-2521160 None:intergenic
CCTTCTTAGAATAATATCTA+TGG 0.346102 8:+2525803 None:intergenic
CTTGTCTATTATGCAAGCTT+GGG 0.346956 8:+2525873 None:intergenic
CCTTGTCTATTATGCAAGCT+TGG 0.350284 8:+2525872 None:intergenic
AATATTGTTAAAATATTGTA+TGG 0.351091 8:+2528485 None:intergenic
TGGTGACAAATCTCCCTCTT+TGG 0.360825 8:-2528583 MsaT041157.1:CDS
AAGAACATGTGACACTATTA+GGG 0.364487 8:+2528298 None:intergenic
AGAATGTTCTCTTGAATTGT+GGG 0.399894 8:+2528441 None:intergenic
ACTCACAATATTGTTGTTCA+AGG 0.402220 8:+2528254 None:intergenic
AGAGATACAAGATCACATTA+TGG 0.415532 8:-2528178 MsaT041157.1:CDS
GAGAATGTTCTCTTGAATTG+TGG 0.419754 8:+2528440 None:intergenic
TTAACTAACCTTCTTCAATC+CGG 0.424112 8:+2528095 None:intergenic
AATGCTGAGGAAACATCTCT+TGG 0.477689 8:-2521195 MsaT041157.1:CDS
CATTTACATATAGACTGATA+AGG 0.479352 8:-2526007 MsaT041157.1:intron
ATGTGAAACAACTTCAAGAA+AGG 0.479598 8:-2525957 MsaT041157.1:CDS
AACCTTCTTCAATCCGGGTA+TGG 0.482099 8:+2528101 None:intergenic
AGCTCGAGAACCATAACAAA+AGG 0.486335 8:-2525927 MsaT041157.1:CDS
TAACTAACCTTCTTCAATCC+GGG 0.489242 8:+2528096 None:intergenic
TTTAGAGTGGATGTTCCAAA+AGG 0.493560 8:+2525640 None:intergenic
ATGGACAAGGAAATTCTCAT+TGG 0.497384 8:-2525754 MsaT041157.1:CDS
GATAGCTTCTTGAAGTATGT+AGG 0.500256 8:+2525984 None:intergenic
ATGACTCTTGCTTCAACCCT+AGG 0.502899 8:+2525775 None:intergenic
TTCTAAGAAGGAACTACCTA+GGG 0.514151 8:-2525791 MsaT041157.1:CDS
AATTATGTTCTTGAGAAATG+AGG 0.519126 8:+2528394 None:intergenic
GCATTGTCTGCCACCATACC+CGG 0.522189 8:-2528114 MsaT041157.1:CDS
TGTGAAACAACTTCAAGAAA+GGG 0.529417 8:-2525956 MsaT041157.1:CDS
CACCATACCCGGATTGAAGA+AGG 0.530151 8:-2528103 MsaT041157.1:intron
AATCCATTGTGAGAAACAAA+AGG 0.531350 8:-2525731 MsaT041157.1:CDS
ATTATGTTCTTGAGAAATGA+GGG 0.546811 8:+2528395 None:intergenic
CCATAGATATTATTCTAAGA+AGG 0.551784 8:-2525803 MsaT041157.1:CDS
TTCTTGAGAAATGAGGGAGT+TGG 0.554351 8:+2528401 None:intergenic
AAAAGTTACAATCATTGCCC+AGG 0.561481 8:-2525620 MsaT041157.1:intron
ATTCTAAGAAGGAACTACCT+AGG 0.562025 8:-2525792 MsaT041157.1:CDS
TGAGAAACAAAAGGATATTG+TGG 0.568495 8:-2525722 MsaT041157.1:CDS
AAACTTGTCTTCATCCAAAG+AGG 0.569979 8:+2528569 None:intergenic
GGAGACAAGTTCTTAGTGAG+AGG 0.572609 8:-2528143 MsaT041157.1:CDS
GAATGTTCTCTTGAATTGTG+GGG 0.574490 8:+2528442 None:intergenic
CTTCTTCAATCCGGGTATGG+TGG 0.579713 8:+2528104 None:intergenic
CCAAGCTTGCATAATAGACA+AGG 0.581224 8:-2525872 MsaT041157.1:CDS
AAAGACAAAATGTATTGAGA+AGG 0.581821 8:+2528345 None:intergenic
TAGGGTTGAAGCAAGAGTCA+TGG 0.596540 8:-2525773 MsaT041157.1:CDS
AACTTGTCTTCATCCAAAGA+GGG 0.612368 8:+2528570 None:intergenic
TCCATGTTATAGGAAATGGT+TGG 0.616814 8:-2528603 None:intergenic
CATGTGACACTATTAGGGGA+AGG 0.617663 8:+2528303 None:intergenic
AATGTTCTCTTGAATTGTGG+GGG 0.666781 8:+2528443 None:intergenic
TGAAGCAAGAGTCATGGACA+AGG 0.676606 8:-2525767 MsaT041157.1:CDS
AGAACATGTGACACTATTAG+GGG 0.723595 8:+2528299 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 2521163 2528610 2521163 ID=MsaG041157
Chr8 mRNA 2521163 2528610 2521163 ID=MsaT041157.1;Parent=MsaG041157
Chr8 exon 2521163 2521258 2521163 ID=MsaT041157.1.exon3;Parent=MsaT041157.1
Chr8 CDS 2521163 2521258 2521163 ID=cds.MsaT041157.1;Parent=MsaT041157.1
Chr8 exon 2525621 2526016 2525621 ID=MsaT041157.1.exon2;Parent=MsaT041157.1
Chr8 CDS 2525621 2526016 2525621 ID=cds.MsaT041157.1;Parent=MsaT041157.1
Chr8 exon 2528104 2528610 2528104 ID=MsaT041157.1.exon1;Parent=MsaT041157.1
Chr8 CDS 2528104 2528610 2528104 ID=cds.MsaT041157.1;Parent=MsaT041157.1
Gene Sequence

>MsaT041157.1

ATGGTTGGTGACAAATCTCCCTCTTTGGATGAAGACAAGTTTCAACAAGAGAATATGCAACAATCATGTTTCTCTTTTGAGGGTGATAATTCTCAATCTTCAACCATACAATATTTTAACAATATTTTAGATAATGTTTGTGTCACCCCCACAATTCAAGAGAACATTCTCAAGAATAGCAATAGTTCCAACTCCCTCATTTCTCAAGAACATAATTGTGCTACTAATTCACAAAAATTAGCACCTTCTCAATACATTTTGTCTTTTGAAAACTCAAGTGTGAAACCTTCCCCTAATAGTGTCACATGTTCTTCTATTATGGTCCAAAAAACAACCTTGAACAACAATATTGTGAGTGAATTACCAAAGACAATTAAGAAAAGAACCAAGAATCTAAGAAGAAACTCTTCAGAGATACAAGATCACATTATGGCTGAGAGAAAAAGGAGACAAGTTCTTAGTGAGAGGTTCATAGCATTGTCTGCCACCATACCCGGATTGAAGAAGACTGATAAGGCCTACATACTTCAAGAAGCTATCAATTATGTGAAACAACTTCAAGAAAGGGTAAATGAGCTCGAGAACCATAACAAAAGGAAAAGAGATTCAGTAATATTCATCAAGAAATCCCAAGCTTGCATAATAGACAAGGAAAAAAGTACTTCATCATGTGAAGAGAATTTTGATAATGATGATGACCATAGATATTATTCTAAGAAGGAACTACCTAGGGTTGAAGCAAGAGTCATGGACAAGGAAATTCTCATTGGAATCCATTGTGAGAAACAAAAGGATATTGTGGTCAGATTAATGGCTTTGCTTCAAAATCTTCATCTATCTCTTGCTAGTAGCAGTGTCTTGCCTTTTGGAACATCCACTCTAAAAGTTACAATCATTGCCCAGATGGATGATGAATTCTGCATGAGCGTCAATGATCTAGTAAAAATGCTGAGGAAACATCTCTTGGAGTCACGTGAGAATCAAAAGAATATTTCTTAG

Protein sequence

>MsaT041157.1

MVGDKSPSLDEDKFQQENMQQSCFSFEGDNSQSSTIQYFNNILDNVCVTPTIQENILKNSNSSNSLISQEHNCATNSQKLAPSQYILSFENSSVKPSPNSVTCSSIMVQKTTLNNNIVSELPKTIKKRTKNLRRNSSEIQDHIMAERKRRQVLSERFIALSATIPGLKKTDKAYILQEAINYVKQLQERVNELENHNKRKRDSVIFIKKSQACIIDKEKSTSSCEENFDNDDDHRYYSKKELPRVEARVMDKEILIGIHCEKQKDIVVRLMALLQNLHLSLASSSVLPFGTSTLKVTIIAQMDDEFCMSVNDLVKMLRKHLLESRENQKNIS*