AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041317

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041317 MtrunA17_Chr8g0339041 95.405 370 17 0 1 370 1 370 0.0 730
MsaG041317 MtrunA17_Chr2g0296121 23.024 291 211 8 63 348 172 454 3.70e-13 70.5
MsaG041317 MtrunA17_Chr2g0296301 20.130 308 233 9 63 363 80 381 5.47e-13 69.7
MsaG041317 MtrunA17_Chr2g0296711 21.622 296 222 7 63 353 99 389 1.45e-12 68.6
MsaG041317 MtrunA17_Chr4g0043941 21.642 268 198 5 63 318 17 284 3.65e-12 66.6
MsaG041317 MtrunA17_Chr2g0296111 21.583 278 206 6 62 333 84 355 9.06e-12 65.9
MsaG041317 MtrunA17_Chr4g0067671 21.502 293 216 7 47 333 72 356 1.11e-11 65.5
MsaG041317 MtrunA17_Chr2g0295731 20.486 288 224 5 62 348 92 375 1.98e-11 64.7
MsaG041317 MtrunA17_Chr2g0296151 22.973 296 207 8 62 348 85 368 2.53e-11 64.7
MsaG041317 MtrunA17_Chr2g0286191 21.154 312 233 9 45 351 57 360 2.63e-11 64.3
MsaG041317 MtrunA17_Chr2g0295741 22.260 292 212 8 63 348 92 374 6.13e-11 63.5
MsaG041317 MtrunA17_Chr2g0295761 20.875 297 219 9 58 347 99 386 6.71e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041317 AT1G79220.1 48.348 333 161 4 44 370 72 399 7.61e-106 317
MsaG041317 AT5G64950.1 25.090 279 183 7 87 348 107 376 1.75e-11 65.5
MsaG041317 AT1G21150.3 21.404 285 216 5 19 297 18 300 3.03e-11 64.7
MsaG041317 AT1G21150.1 22.266 256 191 5 48 297 83 336 5.21e-11 63.9
MsaG041317 AT1G21150.2 22.047 254 194 4 48 297 147 400 7.37e-11 63.9
MsaG041317 AT1G61990.2 21.212 330 205 9 62 349 94 410 8.48e-11 63.5
MsaG041317 AT1G61990.1 21.212 330 205 9 62 349 94 410 8.48e-11 63.5

Find 63 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTTCGATTTCTTTCAAT+TGG 0.227300 8:-4818036 MsaT041317.1:CDS
ACTTCGCTTGCTTAGTGATT+TGG 0.257285 8:-4817767 MsaT041317.1:CDS
ATTGACAATGTCAAAATATT+AGG 0.269638 8:+4817286 None:intergenic
CTTCGCTTGCTTAGTGATTT+GGG 0.280618 8:-4817766 MsaT041317.1:CDS
AGCAATGAAGGTACAGAATA+TGG 0.288554 8:-4817137 MsaT041317.1:CDS
GGAAATTGCTGATGAGTTAA+TGG 0.297355 8:-4817026 MsaT041317.1:CDS
TTGCTTAGTGATTTGGGTTT+AGG 0.299185 8:-4817760 MsaT041317.1:CDS
AAATATGTGGTGACATTAAT+AGG 0.301744 8:-4817403 MsaT041317.1:CDS
TAGCAGTTTCATCAAGGAAT+TGG 0.311012 8:-4816970 MsaT041317.1:CDS
CAAGCCTCTCGTCGAAATTA+TGG 0.312035 8:+4817677 None:intergenic
ATATTTGAAAACAACAGATA+TGG 0.357754 8:+4817190 None:intergenic
TTTGTACAATTTCGAATCCT+TGG 0.360192 8:+4817423 None:intergenic
TTTGACATTGTCAATTGATA+AGG 0.373162 8:-4817278 MsaT041317.1:CDS
TGCAAAATGTTGAAGACAAA+TGG 0.380077 8:-4817903 MsaT041317.1:CDS
TTGCTGTGGTGAATGTAAAA+TGG 0.394132 8:+4817998 None:intergenic
TGCTTCGATTTCTTTCAATT+GGG 0.402159 8:-4818035 MsaT041317.1:CDS
TTCATTGCTAGAAGCACTCT+TGG 0.407140 8:+4817151 None:intergenic
GCGCAATGCGGATCCTTCTC+AGG 0.407457 8:-4817800 MsaT041317.1:CDS
TATGTCATTGTTTGAGTCAA+AGG 0.408420 8:-4817647 MsaT041317.1:CDS
TTGTAAAGATTATCAATTGT+AGG 0.408436 8:-4817726 MsaT041317.1:CDS
CATCAGCAATTTCCTTATCA+TGG 0.411377 8:+4817035 None:intergenic
ATGGAATATATAAGTAGGAT+TGG 0.421574 8:-4817451 MsaT041317.1:CDS
AGCTGGGACTTTCGTCGAAT+AGG 0.435629 8:+4817942 None:intergenic
TGTGGAAACAATTCGCGAAA+AGG 0.448608 8:-4817371 MsaT041317.1:CDS
CCTCAATGCTCTAAGTATTG+AGG 0.451275 8:+4817096 None:intergenic
CGTATTGTTGCACAGTATGA+AGG 0.453789 8:-4817559 MsaT041317.1:CDS
TTCCTTTGATGATGAGAAGA+TGG 0.456305 8:-4817470 MsaT041317.1:CDS
CTCAATGCTCTAAGTATTGA+GGG 0.456627 8:+4817097 None:intergenic
GTTGCACAGTATGAAGGGTT+AGG 0.459567 8:-4817553 MsaT041317.1:CDS
AAGGGTTAGGTATACCGAAA+AGG 0.464523 8:-4817540 MsaT041317.1:CDS
GTCAAAGGAAATGCTTCATA+AGG 0.473131 8:-4817632 MsaT041317.1:CDS
TGGAATATATAAGTAGGATT+GGG 0.487565 8:-4817450 MsaT041317.1:CDS
GATCCGCATTGCGCAAAGAC+GGG 0.492040 8:+4817809 None:intergenic
AGGTATACCGAAAAGGGACT+TGG 0.507671 8:-4817533 MsaT041317.1:CDS
GTTTGTCAGATGCCATGATA+AGG 0.508257 8:-4817047 MsaT041317.1:CDS
TATTTGAAAACAACAGATAT+GGG 0.512551 8:+4817191 None:intergenic
CATATAAAAGTAGTTCAGAA+AGG 0.515005 8:+4816932 None:intergenic
GGATCCGCATTGCGCAAAGA+CGG 0.525836 8:+4817808 None:intergenic
AAGAGTGCTTCTAGCAATGA+AGG 0.527541 8:-4817149 MsaT041317.1:CDS
AGGGTTAGGTATACCGAAAA+GGG 0.527814 8:-4817539 MsaT041317.1:CDS
CAATGAAGGGTTTCTTGCAA+TGG 0.534493 8:+4817609 None:intergenic
AGAAGATGGAATATATAAGT+AGG 0.535407 8:-4817456 MsaT041317.1:CDS
AGAGATTAGCAGTTTCATCA+AGG 0.536690 8:-4816976 MsaT041317.1:CDS
ATATAAAAGTAGTTCAGAAA+GGG 0.539481 8:+4816933 None:intergenic
CTTCATACTGTGCAACAATA+CGG 0.541616 8:+4817560 None:intergenic
TTCGAAATTGTACAAATATG+TGG 0.547821 8:-4817416 MsaT041317.1:CDS
AAGTAGGATTGGGCTTTCCA+AGG 0.560292 8:-4817440 MsaT041317.1:CDS
TGTCAATTGATAAGGTTCAA+AGG 0.565097 8:-4817270 MsaT041317.1:CDS
TTCCATCTTCTCATCATCAA+AGG 0.572098 8:+4817468 None:intergenic
TCAAGGAATTGGACTACTAG+AGG 0.573509 8:-4816959 MsaT041317.1:CDS
CATTGAGGATGTCCGAGGAG+AGG 0.582695 8:-4817081 MsaT041317.1:CDS
GACTTTCGTCGAATAGGTAG+CGG 0.585753 8:+4817948 None:intergenic
ATTAATAGGCATCTCTCGTG+TGG 0.591058 8:-4817389 MsaT041317.1:CDS
GAAAGTCCCAGCTCAAAATG+AGG 0.604863 8:-4817932 MsaT041317.1:CDS
CCTCAATACTTAGAGCATTG+AGG 0.607754 8:-4817096 MsaT041317.1:CDS
GTATTGTTGCACAGTATGAA+GGG 0.608863 8:-4817558 MsaT041317.1:CDS
TTCTCGTCAAATCGTCATCA+CGG 0.617343 8:+4817845 None:intergenic
TTAACCATAATTTCGACGAG+AGG 0.625796 8:-4817681 MsaT041317.1:CDS
GAAGTTCTCGAAATAACAGT+CGG 0.638101 8:+4817490 None:intergenic
AGTTTCGACTGAACCTGAGA+AGG 0.646506 8:+4817787 None:intergenic
AAATGTTGAAGACAAATGGA+AGG 0.655802 8:-4817899 MsaT041317.1:CDS
TAGAGCATTGAGGATGTCCG+AGG 0.681763 8:-4817086 MsaT041317.1:CDS
TTGCCCGTCTTTGCGCAATG+CGG 0.689638 8:-4817812 MsaT041317.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 4816946 4818058 4816946 ID=MsaG041317
Chr8 mRNA 4816946 4818058 4816946 ID=MsaT041317.1;Parent=MsaG041317
Chr8 exon 4816946 4818058 4816946 ID=MsaT041317.1.exon1;Parent=MsaT041317.1
Chr8 CDS 4816946 4818058 4816946 ID=cds.MsaT041317.1;Parent=MsaT041317.1
Gene Sequence

>MsaT041317.1

ATGCTTCGATTTCTTTCAATTGGGTCAAAAAATTTTCACCATTTTACATTCACCACAGCAATTATAAAAGTTTTCCCAGTTTTTACGGCCGCTACCTATTCGACGAAAGTCCCAGCTCAAAATGAGGTTGAATTGCAAAATGTTGAAGACAAATGGAAGGATGCTACTGAAGTTTTGAGTAAATGGGGTTGCCGTGATGACGATTTGACGAGAATATTCACTCGTTGCCCGTCTTTGCGCAATGCGGATCCTTCTCAGGTTCAGTCGAAACTTCGCTTGCTTAGTGATTTGGGTTTAGGCTCAGCTGAGCTTGTAAAGATTATCAATTGTAGGCCTCGATTTTTCAGGACTCGACTTAACCATAATTTCGACGAGAGGCTTGACTCTCTTATGTCATTGTTTGAGTCAAAGGAAATGCTTCATAAGGCCATTGCAAGAAACCCTTCATTGCTGTGTGAAAATAATTATGACATTGACCGTATTGTTGCACAGTATGAAGGGTTAGGTATACCGAAAAGGGACTTGGTTCAAATGATGATTTTGAGACCGACTGTTATTTCGAGAACTTCCTTTGATGATGAGAAGATGGAATATATAAGTAGGATTGGGCTTTCCAAGGATTCGAAATTGTACAAATATGTGGTGACATTAATAGGCATCTCTCGTGTGGAAACAATTCGCGAAAAGGTGTTGAATTTTACAAAGTATGGTTTTTCGGATGATGAAATATTCGGTCTTTTTGGGAAGTCTCCTAATATTTTGACATTGTCAATTGATAAGGTTCAAAGGAACATGACTTTCATTTTAGGCACAATGAAGCTTGAAGCTAACATAATCTTTACGTACCCATATCTGTTGTTTTCAAATATGGAGACTGTTTTGAAGCCAAGAGTGCTTCTAGCAATGAAGGTACAGAATATGGATTCTAATATGAAAACACCCTCAATACTTAGAGCATTGAGGATGTCCGAGGAGAGGTTTTTCAATACGTTTGTCAGATGCCATGATAAGGAAATTGCTGATGAGTTAATGGAATTTTATAAAAGGACAAAACAGGTCAAGAGATTAGCAGTTTCATCAAGGAATTGGACTACTAGAGGATTCCCTTTCTGA
Protein sequence

>MsaT041317.1

MLRFLSIGSKNFHHFTFTTAIIKVFPVFTAATYSTKVPAQNEVELQNVEDKWKDATEVLSKWGCRDDDLTRIFTRCPSLRNADPSQVQSKLRLLSDLGLGSAELVKIINCRPRFFRTRLNHNFDERLDSLMSLFESKEMLHKAIARNPSLLCENNYDIDRIVAQYEGLGIPKRDLVQMMILRPTVISRTSFDDEKMEYISRIGLSKDSKLYKYVVTLIGISRVETIREKVLNFTKYGFSDDEIFGLFGKSPNILTLSIDKVQRNMTFILGTMKLEANIIFTYPYLLFSNMETVLKPRVLLAMKVQNMDSNMKTPSILRALRMSEERFFNTFVRCHDKEIADELMEFYKRTKQVKRLAVSSRNWTTRGFPF*